n m sirna duplexes Search Results


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  • 99
    ATCC hek 293 cells
    FasL 3′-UTR decreases the mRNA and protein levels of a GFP reporter in both Jurkat and <t>HEK</t> 293 cells. A and B , Jurkat ( A ) and HEK 293 ( B ) cells were transfected with pEGFP-C2 control and pEGFP-C2-FasL 3′-UTR. Flow cytometric analysis of
    Hek 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher lipofectamine 2000
    FasL 3′-UTR decreases the mRNA and protein levels of a GFP reporter in both Jurkat and <t>HEK</t> 293 cells. A and B , Jurkat ( A ) and HEK 293 ( B ) cells were transfected with pEGFP-C2 control and pEGFP-C2-FasL 3′-UTR. Flow cytometric analysis of
    Lipofectamine 2000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 466214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher nonspecific sirna
    SIRT6 regulates <t>Nrf2–HK2</t> association. A , Western blot depicting elevated nuclear Nrf2 expression in IL-1β–treated glioma cells under glucose starvation. B , <t>siRNA-mediated</t> knockdown of SIRT6 prevents nuclear Nrf2 accumulation in cells treated with IL-1β under glucose starvation as demonstrated by Western blot analysis. Blots were reprobed for c23 to establish equivalent loading. C , SIRT6 positively regulates nuclear HK2–Nrf2 interaction in the presence of IL-1β in glucose-starved glioma cells. Coimmunoprecipitation shows decreased HK2–Nrf2 association in IL-1β–treated glucose-deprived cells upon siRNA-mediated knockdown of SIRT6. A confounding effect of unequal precipitation on the interaction studies was ruled out by almost equal IgG levels in each condition. Blots are representative images of three independent experiments showing similar results. NSsiRNA , nonspecific siRNA; IP , immunoprecipitation; IB , immunoblotting.
    Nonspecific Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher c jun specific shrna clones
    c-Jun regulates Akt activity via PDK1. A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    C Jun Specific Shrna Clones, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Santa Cruz Biotechnology lkb1 sirna
    CaMKKβ is a key determinant of statin-dependent AMPK and <t>LKB1</t> phosphorylation. In the experiments shown in A and B , cultured endothelial cells were transfected with control or CaMKKβ <t>siRNA;</t> 24 h later simvastatin (10 μ m ) was
    Lkb1 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Amaxa reagent v lentiviral shrna clones
    CaMKKβ is a key determinant of statin-dependent AMPK and <t>LKB1</t> phosphorylation. In the experiments shown in A and B , cultured endothelial cells were transfected with control or CaMKKβ <t>siRNA;</t> 24 h later simvastatin (10 μ m ) was
    Reagent V Lentiviral Shrna Clones, supplied by Amaxa, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Qiagen c jun specific sirna oligonucleotide
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    C Jun Specific Sirna Oligonucleotide, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher ets 1 specific sirna oligonucleotides
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Ets 1 Specific Sirna Oligonucleotides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher lipofectamine rnaimax reagent
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Lipofectamine Rnaimax Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24662 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Amaxa nucleofection
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Nucleofection, supplied by Amaxa, used in various techniques. Bioz Stars score: 94/100, based on 6283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher lipofectamine plus reagent
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Lipofectamine Plus Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Amaxa lipofectamine 2000
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Lipofectamine 2000, supplied by Amaxa, used in various techniques. Bioz Stars score: 93/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Horizon Discovery dharmafect reagent 1
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Dharmafect Reagent 1, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 90/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Horizon Discovery sicontrol scrambled
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Sicontrol Scrambled, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Horizon Discovery pdk1 specific smartpool reagents
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Pdk1 Specific Smartpool Reagents, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher rnaimax oligofectamine
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Rnaimax Oligofectamine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher opti mem
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Opti Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 56618 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega glomax 96 microplate luminometer
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Glomax 96 Microplate Luminometer, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 5542 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega luciferase activity
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Luciferase Activity, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 25594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore poly d lysine
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Poly D Lysine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega dual luciferase assay kit
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Dual Luciferase Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 18463 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore laminin
    c-Jun regulates Akt activity via <t>PDK1.</t> A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or <t>siRNA</t> specific for PDK1 were analyzed by Western
    Laminin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transfection cell medium
    SIRT6 affects cell viability through regulation of redox homeostasis. A , Western blot demonstrating SIRT6 levels in IL-1β–treated glioma cells in the presence or absence of glucose. Blots are representative images of three independent experiments showing similar results. Blots were reprobed for β-actin to establish equivalent loading. Densitometry data depicting -fold change in SIRT6 expression over control under the indicated treatment conditions normalized to corresponding loading controls are shown. siRNA-mediated knockdown of SIRT6 increases ( B ) and SIRT6 overexpression decreases ( C ) viability of glioma cells deprived of glucose and treated with IL-1β as determined by MTS assay. Insets in B and C show knockdown efficiency of SIRT6 siRNA and increased SIRT6 expression upon <t>transfection</t> with SIRT6 overexpression construct. D and E , DHE fluorescence intensity showing that ROS generation in glucose-deprived IL-1β–treated cells is SIRT6-dependent. F , SIRT6 regulates SOD2 expression under a combination of inflammatory and metabolic stresses. Densitometry data depict -fold change in SOD2 expression over control under the indicated treatment conditions normalized to corresponding loading controls. Each data point in the scatter plots represents average absorbance values depicting glioma cell viability ( n = 4) ( B and C ) and fluorescence values depicting ROS levels under the indicated treatment conditions from independent experiments ( n = 3) ( D and E ). − G denotes glucose-free DMEM. NSsiRNA , nonspecific siRNA; SIRT6OE , SIRT6 overexpression. Two-tailed paired Student's t test ( B and C ) and one-way ANOVA (Bonferroni's multiple comparison test) ( A , D , E , and F ) were used for statistical analysis. Error bars represent S.E. *, p
    Transfection Cell Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    FasL 3′-UTR decreases the mRNA and protein levels of a GFP reporter in both Jurkat and HEK 293 cells. A and B , Jurkat ( A ) and HEK 293 ( B ) cells were transfected with pEGFP-C2 control and pEGFP-C2-FasL 3′-UTR. Flow cytometric analysis of

    Journal: The Journal of Biological Chemistry

    Article Title: FasL Expression in Activated T Lymphocytes Involves HuR-mediated Stabilization *

    doi: 10.1074/jbc.M110.137919

    Figure Lengend Snippet: FasL 3′-UTR decreases the mRNA and protein levels of a GFP reporter in both Jurkat and HEK 293 cells. A and B , Jurkat ( A ) and HEK 293 ( B ) cells were transfected with pEGFP-C2 control and pEGFP-C2-FasL 3′-UTR. Flow cytometric analysis of

    Article Snippet: HEK 293 cells were transfected at 20% confluence then retransfected with siRNA 24 h later.

    Techniques: Transfection, Flow Cytometry

    Expression of the GFP-FasL 3′-UTR mRNA and protein in conditions of PKC activation is mediated by HuR. A , HEK 293 cells were transfected with either GFP ( filled bars ) or GFP-FasL 3′-UTR ( open bars ). These cells were treated for 4 h with

    Journal: The Journal of Biological Chemistry

    Article Title: FasL Expression in Activated T Lymphocytes Involves HuR-mediated Stabilization *

    doi: 10.1074/jbc.M110.137919

    Figure Lengend Snippet: Expression of the GFP-FasL 3′-UTR mRNA and protein in conditions of PKC activation is mediated by HuR. A , HEK 293 cells were transfected with either GFP ( filled bars ) or GFP-FasL 3′-UTR ( open bars ). These cells were treated for 4 h with

    Article Snippet: HEK 293 cells were transfected at 20% confluence then retransfected with siRNA 24 h later.

    Techniques: Expressing, Activation Assay, Transfection

    SIRT6 regulates Nrf2–HK2 association. A , Western blot depicting elevated nuclear Nrf2 expression in IL-1β–treated glioma cells under glucose starvation. B , siRNA-mediated knockdown of SIRT6 prevents nuclear Nrf2 accumulation in cells treated with IL-1β under glucose starvation as demonstrated by Western blot analysis. Blots were reprobed for c23 to establish equivalent loading. C , SIRT6 positively regulates nuclear HK2–Nrf2 interaction in the presence of IL-1β in glucose-starved glioma cells. Coimmunoprecipitation shows decreased HK2–Nrf2 association in IL-1β–treated glucose-deprived cells upon siRNA-mediated knockdown of SIRT6. A confounding effect of unequal precipitation on the interaction studies was ruled out by almost equal IgG levels in each condition. Blots are representative images of three independent experiments showing similar results. NSsiRNA , nonspecific siRNA; IP , immunoprecipitation; IB , immunoblotting.

    Journal: The Journal of Biological Chemistry

    Article Title: Hexokinase 2 and nuclear factor erythroid 2–related factor 2 transcriptionally coactivate xanthine oxidoreductase expression in stressed glioma cells

    doi: 10.1074/jbc.M117.816785

    Figure Lengend Snippet: SIRT6 regulates Nrf2–HK2 association. A , Western blot depicting elevated nuclear Nrf2 expression in IL-1β–treated glioma cells under glucose starvation. B , siRNA-mediated knockdown of SIRT6 prevents nuclear Nrf2 accumulation in cells treated with IL-1β under glucose starvation as demonstrated by Western blot analysis. Blots were reprobed for c23 to establish equivalent loading. C , SIRT6 positively regulates nuclear HK2–Nrf2 interaction in the presence of IL-1β in glucose-starved glioma cells. Coimmunoprecipitation shows decreased HK2–Nrf2 association in IL-1β–treated glucose-deprived cells upon siRNA-mediated knockdown of SIRT6. A confounding effect of unequal precipitation on the interaction studies was ruled out by almost equal IgG levels in each condition. Blots are representative images of three independent experiments showing similar results. NSsiRNA , nonspecific siRNA; IP , immunoprecipitation; IB , immunoblotting.

    Article Snippet: Transfection with 70 n m duplex HK2, 50 n m SIRT6, and Nrf2 or nonspecific siRNA (Thermo Fischer Scientific) was carried out using Lipofectamine RNAiMAX reagent (Life Technologies-Invitrogen) as described previously ( ).

    Techniques: Western Blot, Expressing, Immunoprecipitation

    SIRT6 affects cell viability through regulation of redox homeostasis. A , Western blot demonstrating SIRT6 levels in IL-1β–treated glioma cells in the presence or absence of glucose. Blots are representative images of three independent experiments showing similar results. Blots were reprobed for β-actin to establish equivalent loading. Densitometry data depicting -fold change in SIRT6 expression over control under the indicated treatment conditions normalized to corresponding loading controls are shown. siRNA-mediated knockdown of SIRT6 increases ( B ) and SIRT6 overexpression decreases ( C ) viability of glioma cells deprived of glucose and treated with IL-1β as determined by MTS assay. Insets in B and C show knockdown efficiency of SIRT6 siRNA and increased SIRT6 expression upon transfection with SIRT6 overexpression construct. D and E , DHE fluorescence intensity showing that ROS generation in glucose-deprived IL-1β–treated cells is SIRT6-dependent. F , SIRT6 regulates SOD2 expression under a combination of inflammatory and metabolic stresses. Densitometry data depict -fold change in SOD2 expression over control under the indicated treatment conditions normalized to corresponding loading controls. Each data point in the scatter plots represents average absorbance values depicting glioma cell viability ( n = 4) ( B and C ) and fluorescence values depicting ROS levels under the indicated treatment conditions from independent experiments ( n = 3) ( D and E ). − G denotes glucose-free DMEM. NSsiRNA , nonspecific siRNA; SIRT6OE , SIRT6 overexpression. Two-tailed paired Student's t test ( B and C ) and one-way ANOVA (Bonferroni's multiple comparison test) ( A , D , E , and F ) were used for statistical analysis. Error bars represent S.E. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Hexokinase 2 and nuclear factor erythroid 2–related factor 2 transcriptionally coactivate xanthine oxidoreductase expression in stressed glioma cells

    doi: 10.1074/jbc.M117.816785

    Figure Lengend Snippet: SIRT6 affects cell viability through regulation of redox homeostasis. A , Western blot demonstrating SIRT6 levels in IL-1β–treated glioma cells in the presence or absence of glucose. Blots are representative images of three independent experiments showing similar results. Blots were reprobed for β-actin to establish equivalent loading. Densitometry data depicting -fold change in SIRT6 expression over control under the indicated treatment conditions normalized to corresponding loading controls are shown. siRNA-mediated knockdown of SIRT6 increases ( B ) and SIRT6 overexpression decreases ( C ) viability of glioma cells deprived of glucose and treated with IL-1β as determined by MTS assay. Insets in B and C show knockdown efficiency of SIRT6 siRNA and increased SIRT6 expression upon transfection with SIRT6 overexpression construct. D and E , DHE fluorescence intensity showing that ROS generation in glucose-deprived IL-1β–treated cells is SIRT6-dependent. F , SIRT6 regulates SOD2 expression under a combination of inflammatory and metabolic stresses. Densitometry data depict -fold change in SOD2 expression over control under the indicated treatment conditions normalized to corresponding loading controls. Each data point in the scatter plots represents average absorbance values depicting glioma cell viability ( n = 4) ( B and C ) and fluorescence values depicting ROS levels under the indicated treatment conditions from independent experiments ( n = 3) ( D and E ). − G denotes glucose-free DMEM. NSsiRNA , nonspecific siRNA; SIRT6OE , SIRT6 overexpression. Two-tailed paired Student's t test ( B and C ) and one-way ANOVA (Bonferroni's multiple comparison test) ( A , D , E , and F ) were used for statistical analysis. Error bars represent S.E. *, p

    Article Snippet: Transfection with 70 n m duplex HK2, 50 n m SIRT6, and Nrf2 or nonspecific siRNA (Thermo Fischer Scientific) was carried out using Lipofectamine RNAiMAX reagent (Life Technologies-Invitrogen) as described previously ( ).

    Techniques: Western Blot, Expressing, Over Expression, MTS Assay, Transfection, Construct, Fluorescence, Two Tailed Test

    c-Jun regulates Akt activity via PDK1. A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or siRNA specific for PDK1 were analyzed by Western

    Journal: The Journal of Biological Chemistry

    Article Title: c-Jun Regulates Phosphoinositide-dependent Kinase 1 Transcription

    doi: 10.1074/jbc.M109.075630

    Figure Lengend Snippet: c-Jun regulates Akt activity via PDK1. A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or siRNA specific for PDK1 were analyzed by Western

    Article Snippet: siCONTROL (scrambled) and PDK1-specific SMARTpool reagents were used (Dharmacon). c-Jun-specific siRNA oligonucleotide was obtained from Qiagen, and Sp1-specific and Ets-1-specific siRNA oligonucleotides from Ambion. c-Jun-specific shRNA clones were obtained from Open Biosystems.

    Techniques: Activity Assay, Activation Assay, Transfection, Western Blot

    CaMKKβ is a key determinant of statin-dependent AMPK and LKB1 phosphorylation. In the experiments shown in A and B , cultured endothelial cells were transfected with control or CaMKKβ siRNA; 24 h later simvastatin (10 μ m ) was

    Journal: The Journal of Biological Chemistry

    Article Title: Regulation of Rac1 by Simvastatin in Endothelial Cells

    doi: 10.1074/jbc.M808664200

    Figure Lengend Snippet: CaMKKβ is a key determinant of statin-dependent AMPK and LKB1 phosphorylation. In the experiments shown in A and B , cultured endothelial cells were transfected with control or CaMKKβ siRNA; 24 h later simvastatin (10 μ m ) was

    Article Snippet: LKB1 siRNA (Sc-35816) was from Santa Cruz Biotechnology, Inc., and the final siRNA concentration for human umbilical vein endothelial cells (HUVECs) are 100 n m .

    Techniques: Cell Culture, Transfection

    siRNA-mediated LKB1 knockdown attenuates simvastatin-induced AMPK phosphorylation. This figure shows results of an immunoblot experiment performed in HUVECs. Cells were transfected with control or LKB1 siRNA, and 24 h later simvastatin (10 μ

    Journal: The Journal of Biological Chemistry

    Article Title: Regulation of Rac1 by Simvastatin in Endothelial Cells

    doi: 10.1074/jbc.M808664200

    Figure Lengend Snippet: siRNA-mediated LKB1 knockdown attenuates simvastatin-induced AMPK phosphorylation. This figure shows results of an immunoblot experiment performed in HUVECs. Cells were transfected with control or LKB1 siRNA, and 24 h later simvastatin (10 μ

    Article Snippet: LKB1 siRNA (Sc-35816) was from Santa Cruz Biotechnology, Inc., and the final siRNA concentration for human umbilical vein endothelial cells (HUVECs) are 100 n m .

    Techniques: Transfection

    Differential effects of siRNA-mediated Rac1 knockdown on simvastatin-promoted phosphorylation of LKB1 and AMPK. BAECs were transfected with a siRNA construct targeting Rac1 or with control siRNA and treated 24 h later with simvastatin (10 μ

    Journal: The Journal of Biological Chemistry

    Article Title: Regulation of Rac1 by Simvastatin in Endothelial Cells

    doi: 10.1074/jbc.M808664200

    Figure Lengend Snippet: Differential effects of siRNA-mediated Rac1 knockdown on simvastatin-promoted phosphorylation of LKB1 and AMPK. BAECs were transfected with a siRNA construct targeting Rac1 or with control siRNA and treated 24 h later with simvastatin (10 μ

    Article Snippet: LKB1 siRNA (Sc-35816) was from Santa Cruz Biotechnology, Inc., and the final siRNA concentration for human umbilical vein endothelial cells (HUVECs) are 100 n m .

    Techniques: Transfection, Construct

    c-Jun regulates Akt activity via PDK1. A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or siRNA specific for PDK1 were analyzed by Western

    Journal: The Journal of Biological Chemistry

    Article Title: c-Jun Regulates Phosphoinositide-dependent Kinase 1 Transcription

    doi: 10.1074/jbc.M109.075630

    Figure Lengend Snippet: c-Jun regulates Akt activity via PDK1. A , PDK1 is required for Akt phosphorylation and activation. Protein extracts (40 μg) from Lu1205 cells transfected with scrambled ( sc ) oligonucleotides or siRNA specific for PDK1 were analyzed by Western

    Article Snippet: siCONTROL (scrambled) and PDK1-specific SMARTpool reagents were used (Dharmacon). c-Jun-specific siRNA oligonucleotide was obtained from Qiagen, and Sp1-specific and Ets-1-specific siRNA oligonucleotides from Ambion. c-Jun-specific shRNA clones were obtained from Open Biosystems.

    Techniques: Activity Assay, Activation Assay, Transfection, Western Blot

    SIRT6 affects cell viability through regulation of redox homeostasis. A , Western blot demonstrating SIRT6 levels in IL-1β–treated glioma cells in the presence or absence of glucose. Blots are representative images of three independent experiments showing similar results. Blots were reprobed for β-actin to establish equivalent loading. Densitometry data depicting -fold change in SIRT6 expression over control under the indicated treatment conditions normalized to corresponding loading controls are shown. siRNA-mediated knockdown of SIRT6 increases ( B ) and SIRT6 overexpression decreases ( C ) viability of glioma cells deprived of glucose and treated with IL-1β as determined by MTS assay. Insets in B and C show knockdown efficiency of SIRT6 siRNA and increased SIRT6 expression upon transfection with SIRT6 overexpression construct. D and E , DHE fluorescence intensity showing that ROS generation in glucose-deprived IL-1β–treated cells is SIRT6-dependent. F , SIRT6 regulates SOD2 expression under a combination of inflammatory and metabolic stresses. Densitometry data depict -fold change in SOD2 expression over control under the indicated treatment conditions normalized to corresponding loading controls. Each data point in the scatter plots represents average absorbance values depicting glioma cell viability ( n = 4) ( B and C ) and fluorescence values depicting ROS levels under the indicated treatment conditions from independent experiments ( n = 3) ( D and E ). − G denotes glucose-free DMEM. NSsiRNA , nonspecific siRNA; SIRT6OE , SIRT6 overexpression. Two-tailed paired Student's t test ( B and C ) and one-way ANOVA (Bonferroni's multiple comparison test) ( A , D , E , and F ) were used for statistical analysis. Error bars represent S.E. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Hexokinase 2 and nuclear factor erythroid 2–related factor 2 transcriptionally coactivate xanthine oxidoreductase expression in stressed glioma cells

    doi: 10.1074/jbc.M117.816785

    Figure Lengend Snippet: SIRT6 affects cell viability through regulation of redox homeostasis. A , Western blot demonstrating SIRT6 levels in IL-1β–treated glioma cells in the presence or absence of glucose. Blots are representative images of three independent experiments showing similar results. Blots were reprobed for β-actin to establish equivalent loading. Densitometry data depicting -fold change in SIRT6 expression over control under the indicated treatment conditions normalized to corresponding loading controls are shown. siRNA-mediated knockdown of SIRT6 increases ( B ) and SIRT6 overexpression decreases ( C ) viability of glioma cells deprived of glucose and treated with IL-1β as determined by MTS assay. Insets in B and C show knockdown efficiency of SIRT6 siRNA and increased SIRT6 expression upon transfection with SIRT6 overexpression construct. D and E , DHE fluorescence intensity showing that ROS generation in glucose-deprived IL-1β–treated cells is SIRT6-dependent. F , SIRT6 regulates SOD2 expression under a combination of inflammatory and metabolic stresses. Densitometry data depict -fold change in SOD2 expression over control under the indicated treatment conditions normalized to corresponding loading controls. Each data point in the scatter plots represents average absorbance values depicting glioma cell viability ( n = 4) ( B and C ) and fluorescence values depicting ROS levels under the indicated treatment conditions from independent experiments ( n = 3) ( D and E ). − G denotes glucose-free DMEM. NSsiRNA , nonspecific siRNA; SIRT6OE , SIRT6 overexpression. Two-tailed paired Student's t test ( B and C ) and one-way ANOVA (Bonferroni's multiple comparison test) ( A , D , E , and F ) were used for statistical analysis. Error bars represent S.E. *, p

    Article Snippet: 5 × 103 cells were seeded in 96-well plates, and 2 h prior to transfection cell medium was replaced with Opti-MEM (Gibco, Life Technologies).

    Techniques: Western Blot, Expressing, Over Expression, MTS Assay, Transfection, Construct, Fluorescence, Two Tailed Test