n gonorrhoeae Search Results


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  • 99
    ATCC n gonorrhoeae atcc 49226
    Gepotidacin (GSK2140944) reference agar dilution MIC results from an 8-laboratory investigation to determine QC ranges for N. <t>gonorrhoeae</t> ATCC 49226. All results are within the proposed MIC range (0.25 to 1 μg/ml).
    N Gonorrhoeae Atcc 49226, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Gen-Probe n gonorrhoeae
    C. trachomatis and N. <t>gonorrhoeae</t> infections missed by different screening practices. CT, C. trachomatis ; GC, N. gonorrhoeae .
    N Gonorrhoeae, supplied by Gen-Probe, used in various techniques. Bioz Stars score: 92/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Hologic Gen-Probe n gonorrhoeae
    Isolation and identification of N. <t>gonorrhoeae</t>
    N Gonorrhoeae, supplied by Hologic Gen-Probe, used in various techniques. Bioz Stars score: 92/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abbott Laboratories n gonorrhoeae
    Cost-saving ability of pooling of processed urine specimens before performance of the urine LCR test for the detection of N. <t>gonorrhoeae</t> infections. The graph shows the cost in U.S. dollars (USD) per amplification unit dose when the pooling algorithm was used, depending on the number of specimens per pool and taking into account various prevalences of infection in the population screened. A baseline total cost of $6.32 per unit dose was used.
    N Gonorrhoeae, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC n gonorrhoeae
    Normalized absorption spectra of Neisseria <t>gonorrhoeae</t> (ATCC 700825) lysates, porphyrins, and flavins. FAD, flavin adenine dinucleotide; FMN, flavin mononucleotide.
    N Gonorrhoeae, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson n gonorrhoeae
    Immunoproteomics of gonococal OMV. a : SDS-PAGE of OMV preparations from N. <t>gonorrhoeae</t> strains FA1090, MS11, and FA19, stained with Coomassie blue. b : Western blot analysis of mouse sera tested on gonococcal OMV preparations separated by SDS-PAGE. Lane 1, control serum from a mouse immunized with FA1090 OMV plus blank ms, tested against FA1090 OMV; lanes 2–4, serum #1 from a mouse immunized with FA1090 OMV plus IL-12/ms, tested against OMV from FA1090 (lane 2), MS11 (lane 3), or FA19 (lane 4); lane 5, serum #2 from a mouse immunized with FA1090 OMV plus IL-12/ms, tested against OMV from FA1090; lane 6, antibody H5 (anti-porin PIB3) tested against FA1090 OMV. c–e : proteome maps of gonococcal OMV derived from FA1090 ( c ), MS11 ( d ), and FA19 ( e ) revealed by 2D electrophoresis and Flamingo fluorescent staining (left panels) and their corresponding immunoblots (right panels) obtained by probing with mouse serum #2. Immunoreactive spots subjected to MS/MS analysis are labeled as spots 1 and 2 (arrows). Molecular mass marker (kDa) indicated on the left.
    N Gonorrhoeae, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Hologic Inc n gonorrhoeae
    Transcription-mediated amplification detection rates of Chlamydia trachomatis , Neisseria <t>gonorrhoeae</t> , Trichomonas vaginalis , and Mycoplasma genitalium in 1,465 urine, 1,443 pharyngeal, and 823 rectal specimens. †, P
    N Gonorrhoeae, supplied by Hologic Inc, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson n gonorrhoeae amplified dna assay
    Transcription-mediated amplification detection rates of Chlamydia trachomatis , Neisseria <t>gonorrhoeae</t> , Trichomonas vaginalis , and Mycoplasma genitalium in 1,465 urine, 1,443 pharyngeal, and 823 rectal specimens. †, P
    N Gonorrhoeae Amplified Dna Assay, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Freund-Vector n gonorrhoeae
    Immunogenicity of MsrA/B. The titers of the post-immune sera from each mouse immunized with either MsrA/B- Alum or MsrA/B-Freund's were determined by ELISA against (A) purified recombinant MsrA/B for IgG1, G2a, G2b, G3, IgM, or (B) whole cell N. <t>gonorrhoeae</t> 1291 wild type (WT), msr::kan mutant (Δmsr), and complemented (Δmsr_C) strains for IgG. The titer for each of 10 mice are shown with circles, and the geometric mean titer (GMT) and 95% Confidence interval are indicated bars. The titers of pre-immune sera against whole cell N. gonorrhoeae 1291 strains were ≤ 200.
    N Gonorrhoeae, supplied by Freund-Vector, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Copan Diagnostics n gonorrhoeae
    Immunogenicity of MsrA/B. The titers of the post-immune sera from each mouse immunized with either MsrA/B- Alum or MsrA/B-Freund's were determined by ELISA against (A) purified recombinant MsrA/B for IgG1, G2a, G2b, G3, IgM, or (B) whole cell N. <t>gonorrhoeae</t> 1291 wild type (WT), msr::kan mutant (Δmsr), and complemented (Δmsr_C) strains for IgG. The titer for each of 10 mice are shown with circles, and the geometric mean titer (GMT) and 95% Confidence interval are indicated bars. The titers of pre-immune sera against whole cell N. gonorrhoeae 1291 strains were ≤ 200.
    N Gonorrhoeae, supplied by Copan Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Microsoft n gonorrhoeae strain fa1090
    Three-way Mauve alignment of N. <t>gonorrhoeae</t> strains NCCP11945 (top), TCDC-NG08107 (middle), and <t>FA1090</t> (bottom). The numbered blocks represent regions of homology between strains as determined by progressive Mauve alignment on default settings. White vertical lines within blocks represent small localized areas of the genome sequences that have not aligned. The largest of these is the Gonococcal Genetic Island (GGI), which is present in strain NCCP11945 but not the other two strains. Blocks below the central line represent sequences that are inverted in comparison to the strain NCCP11945 arrangement. Homologous blocks are numbered 1 to 14. Blocks 5 and 12 are absent from TCDC-NG08107. Positions of prophage sequences, some of which are fragmented, are indicted: NGOФ1 (Ф1); NGOФ2 (Ф2); NGOФ3 (Ф3); NGOФ4 (Ф4); NGOФ5 (Ф5); NGOФ6 (Ф6); NGOФ7 (Ф7); NGOФ8 (Ф8); and NGOФ9 (Ф9). Labeled are the positions of block 2 flanking IS1106 elements, the block 4 flanking SSREE elements, and the block 11–14 flanking CREE in strain NCCP11945. Note that the chromosomal positions for strain TCDC-NG08107 do not equate to those in GenBank; position 1 was adjusted to be the first base of dnaA to facilitate comparisons.
    N Gonorrhoeae Strain Fa1090, supplied by Microsoft, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Eurofins n gonorrhoeae qpcr assay
    Three-way Mauve alignment of N. <t>gonorrhoeae</t> strains NCCP11945 (top), TCDC-NG08107 (middle), and <t>FA1090</t> (bottom). The numbered blocks represent regions of homology between strains as determined by progressive Mauve alignment on default settings. White vertical lines within blocks represent small localized areas of the genome sequences that have not aligned. The largest of these is the Gonococcal Genetic Island (GGI), which is present in strain NCCP11945 but not the other two strains. Blocks below the central line represent sequences that are inverted in comparison to the strain NCCP11945 arrangement. Homologous blocks are numbered 1 to 14. Blocks 5 and 12 are absent from TCDC-NG08107. Positions of prophage sequences, some of which are fragmented, are indicted: NGOФ1 (Ф1); NGOФ2 (Ф2); NGOФ3 (Ф3); NGOФ4 (Ф4); NGOФ5 (Ф5); NGOФ6 (Ф6); NGOФ7 (Ф7); NGOФ8 (Ф8); and NGOФ9 (Ф9). Labeled are the positions of block 2 flanking IS1106 elements, the block 4 flanking SSREE elements, and the block 11–14 flanking CREE in strain NCCP11945. Note that the chromosomal positions for strain TCDC-NG08107 do not equate to those in GenBank; position 1 was adjusted to be the first base of dnaA to facilitate comparisons.
    N Gonorrhoeae Qpcr Assay, supplied by Eurofins, used in various techniques. Bioz Stars score: 88/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Clinical and Laboratory Standards Institute n gonorrhoeae
    Summary of the number and percentage of TRNG isolates obtained in Korea by year. TRNG, high-level tetracycline-resistant Neisseria <t>gonorrhoeae</t> .
    N Gonorrhoeae, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher n gonorrhoeae
    Summary of the number and percentage of TRNG isolates obtained in Korea by year. TRNG, high-level tetracycline-resistant Neisseria <t>gonorrhoeae</t> .
    N Gonorrhoeae, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Pacific Biosciences n gonorrhoeae strains
    Summary of the number and percentage of TRNG isolates obtained in Korea by year. TRNG, high-level tetracycline-resistant Neisseria <t>gonorrhoeae</t> .
    N Gonorrhoeae Strains, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Trinity Biotech microtrak n gonorrhoeae culture confirmation test
    Summary of the number and percentage of TRNG isolates obtained in Korea by year. TRNG, high-level tetracycline-resistant Neisseria <t>gonorrhoeae</t> .
    Microtrak N Gonorrhoeae Culture Confirmation Test, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC n gonorrhoeae atcc 19424
    Summary of the number and percentage of TRNG isolates obtained in Korea by year. TRNG, high-level tetracycline-resistant Neisseria <t>gonorrhoeae</t> .
    N Gonorrhoeae Atcc 19424, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Glaxo Smith n gonorrhoeae isolates
    Frequency distribution of gepotidacin MICs against baseline urogenital N. <t>gonorrhoeae</t> isolates ( N = 69).
    N Gonorrhoeae Isolates, supplied by Glaxo Smith, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Gepotidacin (GSK2140944) reference agar dilution MIC results from an 8-laboratory investigation to determine QC ranges for N. gonorrhoeae ATCC 49226. All results are within the proposed MIC range (0.25 to 1 μg/ml).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Multicenter Investigation of Gepotidacin (GSK2140944) Agar Dilution Quality Control Determinations for Neisseria gonorrhoeae ATCC 49226

    doi: 10.1128/AAC.00527-16

    Figure Lengend Snippet: Gepotidacin (GSK2140944) reference agar dilution MIC results from an 8-laboratory investigation to determine QC ranges for N. gonorrhoeae ATCC 49226. All results are within the proposed MIC range (0.25 to 1 μg/ml).

    Article Snippet: The geometric mean MIC results only varied from 0.40 to 0.50 μg/ml among the 8 participant sites and ranged from 0.41 to 0.51 μg/ml for the three tested medium lots. shows the gepotidacin MIC distribution for N. gonorrhoeae ATCC 49226 (240 values).

    Techniques:

    Ng-MscS and Ec-MscS are exchangeable and important for survival during hypo-osmotic shock. The survival of N. gonorrhoeae ATCC 49226 and ZJXSH86 wild-type and mutant strains and E. coli MJF465 strains expressing Ng-MscS or Ec-MscS was determined upon exposure for 20 min to hypo-osmotic medium ( N. gonorrhoeae ) or deionized water ( E. coli ) and compared with survival upon exposure to isosmotic medium. Graphs represent the means and standard deviations of data from at least three independent experiments. (A) Survival of N. gonorrhoeae ATCC 49226 (wild type [WT]), ATCC 49226-Δ mscS :: kanR , ATCC 49226-Ng- mscS -C, ATCC 49226-Ng- mscS -C(i), and ATCC 49226-Ec- mscS -C(i) upon osmotic down-shock. (B) Survival of N. gonorrhoeae ZJXSH86 (wild type), ZJXSH86-Δ mscS :: kanR , ZJXSH86-Ng- mscS -C(i), and ZJXSH86-Ec- mscS -C(i) upon osmotic down-shock. (C) Survival of E. coli MJF465 expressing Ng-MscS or Ec-MscS and the empty vector control upon osmotic down-shock. Significant differences were identified by analysis of variance (GraphPad Prism). *, P

    Journal: Infection and Immunity

    Article Title: Identification and Characterization of the Neisseria gonorrhoeae MscS-Like Mechanosensitive Channel

    doi: 10.1128/IAI.00090-18

    Figure Lengend Snippet: Ng-MscS and Ec-MscS are exchangeable and important for survival during hypo-osmotic shock. The survival of N. gonorrhoeae ATCC 49226 and ZJXSH86 wild-type and mutant strains and E. coli MJF465 strains expressing Ng-MscS or Ec-MscS was determined upon exposure for 20 min to hypo-osmotic medium ( N. gonorrhoeae ) or deionized water ( E. coli ) and compared with survival upon exposure to isosmotic medium. Graphs represent the means and standard deviations of data from at least three independent experiments. (A) Survival of N. gonorrhoeae ATCC 49226 (wild type [WT]), ATCC 49226-Δ mscS :: kanR , ATCC 49226-Ng- mscS -C, ATCC 49226-Ng- mscS -C(i), and ATCC 49226-Ec- mscS -C(i) upon osmotic down-shock. (B) Survival of N. gonorrhoeae ZJXSH86 (wild type), ZJXSH86-Δ mscS :: kanR , ZJXSH86-Ng- mscS -C(i), and ZJXSH86-Ec- mscS -C(i) upon osmotic down-shock. (C) Survival of E. coli MJF465 expressing Ng-MscS or Ec-MscS and the empty vector control upon osmotic down-shock. Significant differences were identified by analysis of variance (GraphPad Prism). *, P

    Article Snippet: For all mouse vaginal tract competition assays, derivatives of N. gonorrhoeae strain ATCC 49226 were used, which naturally contain an rpsL allele that confers streptomycin resistance (identical to rpsL of strain FA1090) ( ).

    Techniques: Mutagenesis, Expressing, Plasmid Preparation

    C. trachomatis and N. gonorrhoeae infections missed by different screening practices. CT, C. trachomatis ; GC, N. gonorrhoeae .

    Journal: Journal of Clinical Microbiology

    Article Title: Extragenital Screening Is Essential for Comprehensive Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in the Pediatric Population

    doi: 10.1128/JCM.00335-19

    Figure Lengend Snippet: C. trachomatis and N. gonorrhoeae infections missed by different screening practices. CT, C. trachomatis ; GC, N. gonorrhoeae .

    Article Snippet: We did an in-house validation of the oropharyngeal swab for C. trachomatis and N. gonorrhoeae by testing 64 previously characterized positive and negative samples.

    Techniques:

    N. gonorrhoeae prevalence by activity and test performed.

    Journal: Journal of Clinical Microbiology

    Article Title: Nucleic Acid Amplification Tests for Diagnosis of Neisseria gonorrhoeae Oropharyngeal Infections ▿

    doi: 10.1128/JCM.01581-08

    Figure Lengend Snippet: N. gonorrhoeae prevalence by activity and test performed.

    Article Snippet: The tests performed included N. gonorrhoeae culture, the Gen-Probe Aptima Combo 2 transcription-mediated amplification assay (TMA; version 5.16; Gen-Probe Inc., San Diego, CA), the Roche Cobas Amplicor PCR (version 2.0; Roche Diagnostics Systems Inc., Pleasanton, CA), and the BD ProbeTec ET amplified DNA strand displacement assay (SDA; version 3.11B; Becton Dickinson and Co., Sparks, MD).

    Techniques: Activity Assay

    Isolation and identification of N. gonorrhoeae

    Journal: MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports / Centers for Disease Control

    Article Title: Recommendations for the Laboratory-Based Detection of Chlamydia trachomatis and Neisseria gonorrhoeae -- 2014

    doi:

    Figure Lengend Snippet: Isolation and identification of N. gonorrhoeae

    Article Snippet: Both the PACE and Hybrid Capture assays can detect C. trachomatis or N. gonorrhoeae in a single specimen.

    Techniques: Isolation

    Cost-saving ability of pooling of processed urine specimens before performance of the urine LCR test for the detection of N. gonorrhoeae infections. The graph shows the cost in U.S. dollars (USD) per amplification unit dose when the pooling algorithm was used, depending on the number of specimens per pool and taking into account various prevalences of infection in the population screened. A baseline total cost of $6.32 per unit dose was used.

    Journal: Journal of Clinical Microbiology

    Article Title: Pooling of Urine Samples for Screening for Neisseria gonorrhoeae by Ligase Chain Reaction: Accuracy and Application

    doi:

    Figure Lengend Snippet: Cost-saving ability of pooling of processed urine specimens before performance of the urine LCR test for the detection of N. gonorrhoeae infections. The graph shows the cost in U.S. dollars (USD) per amplification unit dose when the pooling algorithm was used, depending on the number of specimens per pool and taking into account various prevalences of infection in the population screened. A baseline total cost of $6.32 per unit dose was used.

    Article Snippet: The prevalence of N. gonorrhoeae by culture was 5.9% (19 of 322) in females and 31.8% (7 of 22) in males.

    Techniques: Amplification, Infection

    Normalized absorption spectra of Neisseria gonorrhoeae (ATCC 700825) lysates, porphyrins, and flavins. FAD, flavin adenine dinucleotide; FMN, flavin mononucleotide.

    Journal: Lasers in surgery and medicine

    Article Title: Antimicrobial Blue Light Inactivation of Neisseria gonorrhoeae: Roles of Wavelength, Endogenous Photosensitizer, Oxygen, and Reactive Oxygen Species

    doi: 10.1002/lsm.23104

    Figure Lengend Snippet: Normalized absorption spectra of Neisseria gonorrhoeae (ATCC 700825) lysates, porphyrins, and flavins. FAD, flavin adenine dinucleotide; FMN, flavin mononucleotide.

    Article Snippet: The susceptibilities of N. gonorrhoeae (ATCC 700825) in planktonic suspensions to aBL at 405- and 470-nm wavelengths were compared.

    Techniques:

    Confocal images of the co-cultures of Neisseria gonorrhoeae and VK2/E6E7 cells. aBL: treated with 405-nm aBL. +Saponin: incubated with saponin. −Saponin: not incubated with saponin. Extracellular viable bacteria exhibit purple + green, extracellular nonviable bacteria exhibit purple + red. Intracellular viable bacteria exhibit green, and intracellular nonviable bacteria exhibit red in the permeabilized VK2/E6E7 cells or nonviable VK2/E6E7 cells. Solid white arrows: viable intracellular N. gonorrhoeae . Hollow white arrows: nonviable intracellular N. gonorrhoeae . Bar: 5 μm. aBL, antimicrobial blue light; NT, no treatment; PI, propidium iodide.

    Journal: Lasers in surgery and medicine

    Article Title: Antimicrobial Blue Light Inactivation of Neisseria gonorrhoeae: Roles of Wavelength, Endogenous Photosensitizer, Oxygen, and Reactive Oxygen Species

    doi: 10.1002/lsm.23104

    Figure Lengend Snippet: Confocal images of the co-cultures of Neisseria gonorrhoeae and VK2/E6E7 cells. aBL: treated with 405-nm aBL. +Saponin: incubated with saponin. −Saponin: not incubated with saponin. Extracellular viable bacteria exhibit purple + green, extracellular nonviable bacteria exhibit purple + red. Intracellular viable bacteria exhibit green, and intracellular nonviable bacteria exhibit red in the permeabilized VK2/E6E7 cells or nonviable VK2/E6E7 cells. Solid white arrows: viable intracellular N. gonorrhoeae . Hollow white arrows: nonviable intracellular N. gonorrhoeae . Bar: 5 μm. aBL, antimicrobial blue light; NT, no treatment; PI, propidium iodide.

    Article Snippet: The susceptibilities of N. gonorrhoeae (ATCC 700825) in planktonic suspensions to aBL at 405- and 470-nm wavelengths were compared.

    Techniques: Incubation

    Effects of hypoxic condition on the inactivation efficiency of 405-nm aBL on Neisseria gonorrhoeae (ATCC 700825). Bars denote standard error of the means. aBL, antimicrobial blue light; CFU, colony forming units.

    Journal: Lasers in surgery and medicine

    Article Title: Antimicrobial Blue Light Inactivation of Neisseria gonorrhoeae: Roles of Wavelength, Endogenous Photosensitizer, Oxygen, and Reactive Oxygen Species

    doi: 10.1002/lsm.23104

    Figure Lengend Snippet: Effects of hypoxic condition on the inactivation efficiency of 405-nm aBL on Neisseria gonorrhoeae (ATCC 700825). Bars denote standard error of the means. aBL, antimicrobial blue light; CFU, colony forming units.

    Article Snippet: The susceptibilities of N. gonorrhoeae (ATCC 700825) in planktonic suspensions to aBL at 405- and 470-nm wavelengths were compared.

    Techniques:

    Inactivation of Neisseria gonorrhoeae (ATCC 700825) by 405-nm aBL (60 mW/cm 2 , for 9, 18, 27, 36, 45, and 54 J/cm 2 ) and 470-nm aBL (60 mW/cm 2 , for 18, 54, 90, 126, and 234 J/cm 2 ). Bars denote standard error of the means. aBL, antimicrobial blue light; CFU, colony forming units.

    Journal: Lasers in surgery and medicine

    Article Title: Antimicrobial Blue Light Inactivation of Neisseria gonorrhoeae: Roles of Wavelength, Endogenous Photosensitizer, Oxygen, and Reactive Oxygen Species

    doi: 10.1002/lsm.23104

    Figure Lengend Snippet: Inactivation of Neisseria gonorrhoeae (ATCC 700825) by 405-nm aBL (60 mW/cm 2 , for 9, 18, 27, 36, 45, and 54 J/cm 2 ) and 470-nm aBL (60 mW/cm 2 , for 18, 54, 90, 126, and 234 J/cm 2 ). Bars denote standard error of the means. aBL, antimicrobial blue light; CFU, colony forming units.

    Article Snippet: The susceptibilities of N. gonorrhoeae (ATCC 700825) in planktonic suspensions to aBL at 405- and 470-nm wavelengths were compared.

    Techniques:

    Confocal images of vaginal epithelial cells (VK2/E6E7) infected with Neisseria gonorrhoeae (ATCC 700825). aBL: treated with 405-nm aBL. Viable VK2/E6E7 cells and N. gonorrhoeae were stained green by SYTO9, and nonviable VK2/E6E7 cells and N. gonorrhoeae were stained red by PI. A few, spontaneously dead VK2/E6E7 cells and N. gonorrhoeae cells were observed in the culture without aBL irradiation. Bar: 5 μm. aBL, antimicrobial blue light; NT, no treatment; PI, propidium iodide.

    Journal: Lasers in surgery and medicine

    Article Title: Antimicrobial Blue Light Inactivation of Neisseria gonorrhoeae: Roles of Wavelength, Endogenous Photosensitizer, Oxygen, and Reactive Oxygen Species

    doi: 10.1002/lsm.23104

    Figure Lengend Snippet: Confocal images of vaginal epithelial cells (VK2/E6E7) infected with Neisseria gonorrhoeae (ATCC 700825). aBL: treated with 405-nm aBL. Viable VK2/E6E7 cells and N. gonorrhoeae were stained green by SYTO9, and nonviable VK2/E6E7 cells and N. gonorrhoeae were stained red by PI. A few, spontaneously dead VK2/E6E7 cells and N. gonorrhoeae cells were observed in the culture without aBL irradiation. Bar: 5 μm. aBL, antimicrobial blue light; NT, no treatment; PI, propidium iodide.

    Article Snippet: The susceptibilities of N. gonorrhoeae (ATCC 700825) in planktonic suspensions to aBL at 405- and 470-nm wavelengths were compared.

    Techniques: Infection, Staining, Irradiation

    The percentage of nonviable Neisseria gonorrhoeae cells in the co-cultures of N. gonorrhoeae and VK2/E6E7 cells. aBL: treated with 405-nm aBL with exposure of 108 J/cm 2 at 60 mW/cm 2 . P

    Journal: Lasers in surgery and medicine

    Article Title: Antimicrobial Blue Light Inactivation of Neisseria gonorrhoeae: Roles of Wavelength, Endogenous Photosensitizer, Oxygen, and Reactive Oxygen Species

    doi: 10.1002/lsm.23104

    Figure Lengend Snippet: The percentage of nonviable Neisseria gonorrhoeae cells in the co-cultures of N. gonorrhoeae and VK2/E6E7 cells. aBL: treated with 405-nm aBL with exposure of 108 J/cm 2 at 60 mW/cm 2 . P

    Article Snippet: The susceptibilities of N. gonorrhoeae (ATCC 700825) in planktonic suspensions to aBL at 405- and 470-nm wavelengths were compared.

    Techniques:

    Biofilm produced by N. gonorrhoeae on cover glass after 24 h visualized by Field Emission Scanning Electron Microscopy. (A) Biofilm formed by the wt FA1090 strain; (B) Biofilm formed by the N. gonorrhoeae ngoAXmod::km mutant and (C) Biofilm formed by the N. gonorrhoeae igatrpb::ngoAXmod mutant. Experiments were triplicated and representative photographs are shown.

    Journal: Frontiers in Microbiology

    Article Title: Type III Methyltransferase M.NgoAX from Neisseria gonorrhoeae FA1090 Regulates Biofilm Formation and Interactions with Human Cells

    doi: 10.3389/fmicb.2015.01426

    Figure Lengend Snippet: Biofilm produced by N. gonorrhoeae on cover glass after 24 h visualized by Field Emission Scanning Electron Microscopy. (A) Biofilm formed by the wt FA1090 strain; (B) Biofilm formed by the N. gonorrhoeae ngoAXmod::km mutant and (C) Biofilm formed by the N. gonorrhoeae igatrpb::ngoAXmod mutant. Experiments were triplicated and representative photographs are shown.

    Article Snippet: To construct the N. gonorrhoeae complementation mutant, the ngoAXmod gene was amplified from chromosomal DNA of N. gonorrhoeae FA1090 (Gene Bank: AE004969, ATCC 700825) by PCR with Smamod and Nhemod primers (see Supplementary Table for primer sequences), using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies), according to the manufacturer’s protocol.

    Techniques: Produced, Electron Microscopy, Mutagenesis

    Efficacy of REDX05931 in a mouse model of N. gonorrhoeae ATCC 700825 infection 24 h (A) and 7 days (B) after treatment. Statistical analyses were performed using the Student t test: *, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Efficacy of a Novel Tricyclic Topoisomerase Inhibitor in a Murine Model of Neisseria gonorrhoeae Infection

    doi: 10.1128/AAC.00913-16

    Figure Lengend Snippet: Efficacy of REDX05931 in a mouse model of N. gonorrhoeae ATCC 700825 infection 24 h (A) and 7 days (B) after treatment. Statistical analyses were performed using the Student t test: *, P

    Article Snippet: The MIC of REDX05931 was determined to be 0.03 μg/ml against N. gonorrhoeae ATCC 700825.

    Techniques: Infection

    Identification of the ModA13 recognition methylation target sequence. (A) Apo I restriction digest of plasmid pCmGFP isolated from FA1090 modA13 ON and FA1090 modA13::kan cells. The modA13 ON lane shows the presence of a 722-bp fragment that results from lack of restriction at a single Apo I restriction site. In the modA13::kan lane, this fragment is cut into fragments of 527 and 195 bp. (B) The Apo I recognition sequence showing percentage inhibition of restriction by methylation of each adenosine as indicated by REBASE [27] , and a schematic diagram of the 722 bp pair fragment showing the Apo I recognition site, overlapping with a putative ModA13 recognition site. The central panels show Southern blots of chromosomal DNA extracted from modA13 ON and modA13::kan FA1090 cells. (C) DNA digested with Apo I and probed with a PCR product containing an ApoI /AGAAA overlap showed inhibition of digestion in the modA13 ON lane compared to the modA13::kan lane. (D) DNA digested with Alu I and probed with a PCR product containing an Alu I/AGAAA overlap showing no difference in restriction between the modA13 methylated and unmethylated chromosomes. (E) DNA digested with Rsa I and Hind III, and probed with a PCR product containing a Hind III/AGAAA overlap, showed restriction is inhibited in the modA13 ON lane as compared to the modA13::kan lane. Below each blot is the recognition site for each of the restriction enzymes used, and their known sensitivities to adenosine methylation as supplied by REBASE in the case of Apo I and Hind III [27] . Schematics of the probes used in each blot include the coordinates of the FA1090 genome to which the primers bind (see Table S7 ) and the overlap of the restriction enzyme recognition sequence with that of ModA13. (F) Chromosomal DNA extracted from N. gonorrhoeae strains FA1090 modA13 ON, modA13 OFF, modA13::kan , and 96D551 modA12 ON and modA12::kan cells, digested with Apo I and probed as in (C).

    Journal: PLoS Pathogens

    Article Title: Phasevarions Mediate Random Switching of Gene Expression in Pathogenic Neisseria

    doi: 10.1371/journal.ppat.1000400

    Figure Lengend Snippet: Identification of the ModA13 recognition methylation target sequence. (A) Apo I restriction digest of plasmid pCmGFP isolated from FA1090 modA13 ON and FA1090 modA13::kan cells. The modA13 ON lane shows the presence of a 722-bp fragment that results from lack of restriction at a single Apo I restriction site. In the modA13::kan lane, this fragment is cut into fragments of 527 and 195 bp. (B) The Apo I recognition sequence showing percentage inhibition of restriction by methylation of each adenosine as indicated by REBASE [27] , and a schematic diagram of the 722 bp pair fragment showing the Apo I recognition site, overlapping with a putative ModA13 recognition site. The central panels show Southern blots of chromosomal DNA extracted from modA13 ON and modA13::kan FA1090 cells. (C) DNA digested with Apo I and probed with a PCR product containing an ApoI /AGAAA overlap showed inhibition of digestion in the modA13 ON lane compared to the modA13::kan lane. (D) DNA digested with Alu I and probed with a PCR product containing an Alu I/AGAAA overlap showing no difference in restriction between the modA13 methylated and unmethylated chromosomes. (E) DNA digested with Rsa I and Hind III, and probed with a PCR product containing a Hind III/AGAAA overlap, showed restriction is inhibited in the modA13 ON lane as compared to the modA13::kan lane. Below each blot is the recognition site for each of the restriction enzymes used, and their known sensitivities to adenosine methylation as supplied by REBASE in the case of Apo I and Hind III [27] . Schematics of the probes used in each blot include the coordinates of the FA1090 genome to which the primers bind (see Table S7 ) and the overlap of the restriction enzyme recognition sequence with that of ModA13. (F) Chromosomal DNA extracted from N. gonorrhoeae strains FA1090 modA13 ON, modA13 OFF, modA13::kan , and 96D551 modA12 ON and modA12::kan cells, digested with Apo I and probed as in (C).

    Article Snippet: Each microarray consists of 6,389 70mer oligonucleotides representing open reading frames (ORFs) from N. gonorrhoeae strains FA1090 and ATCC 700825 (reference strain), as well as N. meningitidis strains Z2491 (serogroup A) and MC58 (serogroup B).

    Techniques: Methylation, Sequencing, Plasmid Preparation, Isolation, Inhibition, Polymerase Chain Reaction

    Immunoproteomics of gonococal OMV. a : SDS-PAGE of OMV preparations from N. gonorrhoeae strains FA1090, MS11, and FA19, stained with Coomassie blue. b : Western blot analysis of mouse sera tested on gonococcal OMV preparations separated by SDS-PAGE. Lane 1, control serum from a mouse immunized with FA1090 OMV plus blank ms, tested against FA1090 OMV; lanes 2–4, serum #1 from a mouse immunized with FA1090 OMV plus IL-12/ms, tested against OMV from FA1090 (lane 2), MS11 (lane 3), or FA19 (lane 4); lane 5, serum #2 from a mouse immunized with FA1090 OMV plus IL-12/ms, tested against OMV from FA1090; lane 6, antibody H5 (anti-porin PIB3) tested against FA1090 OMV. c–e : proteome maps of gonococcal OMV derived from FA1090 ( c ), MS11 ( d ), and FA19 ( e ) revealed by 2D electrophoresis and Flamingo fluorescent staining (left panels) and their corresponding immunoblots (right panels) obtained by probing with mouse serum #2. Immunoreactive spots subjected to MS/MS analysis are labeled as spots 1 and 2 (arrows). Molecular mass marker (kDa) indicated on the left.

    Journal: Mucosal immunology

    Article Title: Experimental Vaccine Induces Th1-driven Immune Responses and Resistance to Neisseria gonorrhoeae Infection in a Murine Model

    doi: 10.1038/mi.2017.11

    Figure Lengend Snippet: Immunoproteomics of gonococal OMV. a : SDS-PAGE of OMV preparations from N. gonorrhoeae strains FA1090, MS11, and FA19, stained with Coomassie blue. b : Western blot analysis of mouse sera tested on gonococcal OMV preparations separated by SDS-PAGE. Lane 1, control serum from a mouse immunized with FA1090 OMV plus blank ms, tested against FA1090 OMV; lanes 2–4, serum #1 from a mouse immunized with FA1090 OMV plus IL-12/ms, tested against OMV from FA1090 (lane 2), MS11 (lane 3), or FA19 (lane 4); lane 5, serum #2 from a mouse immunized with FA1090 OMV plus IL-12/ms, tested against OMV from FA1090; lane 6, antibody H5 (anti-porin PIB3) tested against FA1090 OMV. c–e : proteome maps of gonococcal OMV derived from FA1090 ( c ), MS11 ( d ), and FA19 ( e ) revealed by 2D electrophoresis and Flamingo fluorescent staining (left panels) and their corresponding immunoblots (right panels) obtained by probing with mouse serum #2. Immunoreactive spots subjected to MS/MS analysis are labeled as spots 1 and 2 (arrows). Molecular mass marker (kDa) indicated on the left.

    Article Snippet: N. gonorrhoeae was cultured on GC agar supplemented with hemoglobin and Isovitalex (BD Diagnostic Systems, Franklin Lakes, NJ) and the resultant growth was checked for colony morphology consistent with Opa protein and pilus expression.

    Techniques: SDS Page, Staining, Western Blot, Mass Spectrometry, Derivative Assay, Two-Dimensional Gel Electrophoresis, Labeling, Marker

    I.vag immunization with gonococcal OMV plus IL-12/ms induced resistance to genital infection with N. gonorrhoeae , and generated an immune response. a : Mice were immunized 3 times at 7-day intervals with OMV (40µg protein) from strain FA1090 plus control (blank) ms or IL-12/ms (1µg IL-12); control mice were sham-immunized with either blank ms, or with IL-12/ms alone. Two weeks after the last immunization, all mice were challenged by i.vag. inoculation with N. gonorrhoeae strain FA1090 (5 × 10 6 CFU), and infection was monitored by vaginal swabbing and plating. Left panel: recovery (CFU) of N. gonorrhoeae (mean ±SEM, N=8 mice), * P

    Journal: Mucosal immunology

    Article Title: Experimental Vaccine Induces Th1-driven Immune Responses and Resistance to Neisseria gonorrhoeae Infection in a Murine Model

    doi: 10.1038/mi.2017.11

    Figure Lengend Snippet: I.vag immunization with gonococcal OMV plus IL-12/ms induced resistance to genital infection with N. gonorrhoeae , and generated an immune response. a : Mice were immunized 3 times at 7-day intervals with OMV (40µg protein) from strain FA1090 plus control (blank) ms or IL-12/ms (1µg IL-12); control mice were sham-immunized with either blank ms, or with IL-12/ms alone. Two weeks after the last immunization, all mice were challenged by i.vag. inoculation with N. gonorrhoeae strain FA1090 (5 × 10 6 CFU), and infection was monitored by vaginal swabbing and plating. Left panel: recovery (CFU) of N. gonorrhoeae (mean ±SEM, N=8 mice), * P

    Article Snippet: N. gonorrhoeae was cultured on GC agar supplemented with hemoglobin and Isovitalex (BD Diagnostic Systems, Franklin Lakes, NJ) and the resultant growth was checked for colony morphology consistent with Opa protein and pilus expression.

    Techniques: Mass Spectrometry, Infection, Generated, Mouse Assay

    Resistance to challenge induced by immunization with gonococcal OMV plus IL-12/ms depended on IFNγ and B cells. a : Course of infection (FA1090) in IFNγ-ko vs wild-type mice immunized with FA1090 OMV; left panel, recovery (CFU) of N. gonorrhoeae (mean ±SEM, N=8 mice), • P

    Journal: Mucosal immunology

    Article Title: Experimental Vaccine Induces Th1-driven Immune Responses and Resistance to Neisseria gonorrhoeae Infection in a Murine Model

    doi: 10.1038/mi.2017.11

    Figure Lengend Snippet: Resistance to challenge induced by immunization with gonococcal OMV plus IL-12/ms depended on IFNγ and B cells. a : Course of infection (FA1090) in IFNγ-ko vs wild-type mice immunized with FA1090 OMV; left panel, recovery (CFU) of N. gonorrhoeae (mean ±SEM, N=8 mice), • P

    Article Snippet: N. gonorrhoeae was cultured on GC agar supplemented with hemoglobin and Isovitalex (BD Diagnostic Systems, Franklin Lakes, NJ) and the resultant growth was checked for colony morphology consistent with Opa protein and pilus expression.

    Techniques: Mass Spectrometry, Infection, Mouse Assay

    Resistance to gonococcal (FA1090) challenge persisted for at least 6 months after immunization with two doses of gonococcal (FA1090) OMV plus IL-12/ms. Left panel: recovery (CFU) of N. gonorrhoeae (mean ±SEM, N=8 mice), * P

    Journal: Mucosal immunology

    Article Title: Experimental Vaccine Induces Th1-driven Immune Responses and Resistance to Neisseria gonorrhoeae Infection in a Murine Model

    doi: 10.1038/mi.2017.11

    Figure Lengend Snippet: Resistance to gonococcal (FA1090) challenge persisted for at least 6 months after immunization with two doses of gonococcal (FA1090) OMV plus IL-12/ms. Left panel: recovery (CFU) of N. gonorrhoeae (mean ±SEM, N=8 mice), * P

    Article Snippet: N. gonorrhoeae was cultured on GC agar supplemented with hemoglobin and Isovitalex (BD Diagnostic Systems, Franklin Lakes, NJ) and the resultant growth was checked for colony morphology consistent with Opa protein and pilus expression.

    Techniques: Mass Spectrometry, Mouse Assay

    Resistance to heterologous gonococcal challenge. a : One month after immunization with FA1090 OMV plus IL-12/ms or blank ms, mice were challenged with N. gonorrhoeae strain FA1090 (homologous challenge) or strain MS11 (heterologous challenge). Left panel: recovery (CFU) of N. gonorrhoeae (mean ±SEM, N=8 mice), * P

    Journal: Mucosal immunology

    Article Title: Experimental Vaccine Induces Th1-driven Immune Responses and Resistance to Neisseria gonorrhoeae Infection in a Murine Model

    doi: 10.1038/mi.2017.11

    Figure Lengend Snippet: Resistance to heterologous gonococcal challenge. a : One month after immunization with FA1090 OMV plus IL-12/ms or blank ms, mice were challenged with N. gonorrhoeae strain FA1090 (homologous challenge) or strain MS11 (heterologous challenge). Left panel: recovery (CFU) of N. gonorrhoeae (mean ±SEM, N=8 mice), * P

    Article Snippet: N. gonorrhoeae was cultured on GC agar supplemented with hemoglobin and Isovitalex (BD Diagnostic Systems, Franklin Lakes, NJ) and the resultant growth was checked for colony morphology consistent with Opa protein and pilus expression.

    Techniques: Mass Spectrometry, Mouse Assay

    Transcription-mediated amplification detection rates of Chlamydia trachomatis , Neisseria gonorrhoeae , Trichomonas vaginalis , and Mycoplasma genitalium in 1,465 urine, 1,443 pharyngeal, and 823 rectal specimens. †, P

    Journal: Journal of Clinical Microbiology

    Article Title: Expansion of Comprehensive Screening of Male Sexually Transmitted Infection Clinic Attendees with Mycoplasma genitalium and Trichomonas vaginalis Molecular Assessment: a Retrospective Analysis

    doi: 10.1128/JCM.01625-16

    Figure Lengend Snippet: Transcription-mediated amplification detection rates of Chlamydia trachomatis , Neisseria gonorrhoeae , Trichomonas vaginalis , and Mycoplasma genitalium in 1,465 urine, 1,443 pharyngeal, and 823 rectal specimens. †, P

    Article Snippet: C. trachomatis - and N. gonorrhoeae -specific analyses from all collections were performed with the Aptima Combo 2 assay (Hologic), including laboratory-validated assessments of pharyngeal and rectal specimens.

    Techniques: Amplification

    Immunogenicity of MsrA/B. The titers of the post-immune sera from each mouse immunized with either MsrA/B- Alum or MsrA/B-Freund's were determined by ELISA against (A) purified recombinant MsrA/B for IgG1, G2a, G2b, G3, IgM, or (B) whole cell N. gonorrhoeae 1291 wild type (WT), msr::kan mutant (Δmsr), and complemented (Δmsr_C) strains for IgG. The titer for each of 10 mice are shown with circles, and the geometric mean titer (GMT) and 95% Confidence interval are indicated bars. The titers of pre-immune sera against whole cell N. gonorrhoeae 1291 strains were ≤ 200.

    Journal: Frontiers in Immunology

    Article Title: The Neisseria gonorrhoeae Methionine Sulfoxide Reductase (MsrA/B) Is a Surface Exposed, Immunogenic, Vaccine Candidate

    doi: 10.3389/fimmu.2019.00137

    Figure Lengend Snippet: Immunogenicity of MsrA/B. The titers of the post-immune sera from each mouse immunized with either MsrA/B- Alum or MsrA/B-Freund's were determined by ELISA against (A) purified recombinant MsrA/B for IgG1, G2a, G2b, G3, IgM, or (B) whole cell N. gonorrhoeae 1291 wild type (WT), msr::kan mutant (Δmsr), and complemented (Δmsr_C) strains for IgG. The titer for each of 10 mice are shown with circles, and the geometric mean titer (GMT) and 95% Confidence interval are indicated bars. The titers of pre-immune sera against whole cell N. gonorrhoeae 1291 strains were ≤ 200.

    Article Snippet: However, in N. gonorrhoeae the MsrA, MsrB and thioredoxin enzymatic functions are present in a single protein, MsrA/B, that is located in the outer membrane ( ).

    Techniques: Enzyme-linked Immunosorbent Assay, Purification, Recombinant, Mutagenesis, Mouse Assay

    Surface localization of MsrA/B. (A) Whole cell ELISA of the wild type (WT) and msr::kan mutant (Δ msr ) strains of N. gonorrhoeae 1291 and N. meningitidis MC58¢3, with anti-MsrA/B antibodies. The negative control containing secondary antibody only (control), is also shown. The graph shows the average absorbance at 450 nm from three independent replicates, ± one standard deviation. (B) Western blot analysis of trypsin treated (20 μg, 10 μg) and untreated (0 μg) whole cell N. gonorrhoeae 1291 and N. meningitidis MC58¢3, probed with antibodies to MsrA/B, the meningococcal surface protein PorA, and the intracellular protein GNA2091. No significant differences were seen in CFUs/ml at t0 vs. 60 min from samples taken at time 0 and 60 min (two-tailed unpaired Student's t -test p > 1.5), indicating that no cell lysis occurred during the assay (see Figure S3 ).

    Journal: Frontiers in Immunology

    Article Title: The Neisseria gonorrhoeae Methionine Sulfoxide Reductase (MsrA/B) Is a Surface Exposed, Immunogenic, Vaccine Candidate

    doi: 10.3389/fimmu.2019.00137

    Figure Lengend Snippet: Surface localization of MsrA/B. (A) Whole cell ELISA of the wild type (WT) and msr::kan mutant (Δ msr ) strains of N. gonorrhoeae 1291 and N. meningitidis MC58¢3, with anti-MsrA/B antibodies. The negative control containing secondary antibody only (control), is also shown. The graph shows the average absorbance at 450 nm from three independent replicates, ± one standard deviation. (B) Western blot analysis of trypsin treated (20 μg, 10 μg) and untreated (0 μg) whole cell N. gonorrhoeae 1291 and N. meningitidis MC58¢3, probed with antibodies to MsrA/B, the meningococcal surface protein PorA, and the intracellular protein GNA2091. No significant differences were seen in CFUs/ml at t0 vs. 60 min from samples taken at time 0 and 60 min (two-tailed unpaired Student's t -test p > 1.5), indicating that no cell lysis occurred during the assay (see Figure S3 ).

    Article Snippet: However, in N. gonorrhoeae the MsrA, MsrB and thioredoxin enzymatic functions are present in a single protein, MsrA/B, that is located in the outer membrane ( ).

    Techniques: Enzyme-linked Immunosorbent Assay, Mutagenesis, Negative Control, Standard Deviation, Western Blot, Two Tailed Test, Lysis

    Expression of MsrA/B in a panel of gonococcal strains. Western blot analysis of MsrA/B expression in a panel of N. gonorrhoeae strains, including the 1291 wild type and msr::kan mutant (1291Δ msr ), and 20 clinical isolates from mucosal and disseminated gonococcal infections.

    Journal: Frontiers in Immunology

    Article Title: The Neisseria gonorrhoeae Methionine Sulfoxide Reductase (MsrA/B) Is a Surface Exposed, Immunogenic, Vaccine Candidate

    doi: 10.3389/fimmu.2019.00137

    Figure Lengend Snippet: Expression of MsrA/B in a panel of gonococcal strains. Western blot analysis of MsrA/B expression in a panel of N. gonorrhoeae strains, including the 1291 wild type and msr::kan mutant (1291Δ msr ), and 20 clinical isolates from mucosal and disseminated gonococcal infections.

    Article Snippet: However, in N. gonorrhoeae the MsrA, MsrB and thioredoxin enzymatic functions are present in a single protein, MsrA/B, that is located in the outer membrane ( ).

    Techniques: Expressing, Western Blot, Mutagenesis

    Functional activity of MsrA/B antisera against N. gonorrhoeae . (A) Serum bactericidal activity. The survival of the N. gonorrhoeae in the presence of two-fold dilutions of heat inactivated pre-immune (light gray) or α-MsrA/B (dark gray) sera, plus 10% normal human serum as a complement source is shown. (B) Opsonophagocytic activity. The survival of the N. gonorrhoeae in the presence of two-fold dilutions of heat inactivated pre-immune (light gray) or α-MsrA/B (dark gray) sera, plus primary human PMNs and 10% normal human serum as a complement source is shown. For (A,B) , data represent the mean survival (±1 standard deviation) for triplicate samples, as a percentage of bacteria in the absence of antibody (the no antibody control (white) set at 100%, represents 2.0 × 10 3 CFU for SBA and 3.5 × 10 3 CFU for OPA). (C) Blocking of MsrA/B binding to Met(O). Surface plasmon resonance (SPR) of MsrA/B interaction with Met(O) was performed in the presence of pre-immune (light gray) or α-MsrA/B (dark gray) sera. Data represents the mean MsrA/B-Met(O) binding (±1 standard deviation) for triplicate samples, as a percentage of MsrA/B-Met(O) binding in the absence of antibody (the no antibody control (white) set at 100%, represents a K D of 15.4 ± 3.7 nM). For (A–C) , statistically significant differences relative to the no serum controls, using a two-tailed Student's t -test are indicated: * P

    Journal: Frontiers in Immunology

    Article Title: The Neisseria gonorrhoeae Methionine Sulfoxide Reductase (MsrA/B) Is a Surface Exposed, Immunogenic, Vaccine Candidate

    doi: 10.3389/fimmu.2019.00137

    Figure Lengend Snippet: Functional activity of MsrA/B antisera against N. gonorrhoeae . (A) Serum bactericidal activity. The survival of the N. gonorrhoeae in the presence of two-fold dilutions of heat inactivated pre-immune (light gray) or α-MsrA/B (dark gray) sera, plus 10% normal human serum as a complement source is shown. (B) Opsonophagocytic activity. The survival of the N. gonorrhoeae in the presence of two-fold dilutions of heat inactivated pre-immune (light gray) or α-MsrA/B (dark gray) sera, plus primary human PMNs and 10% normal human serum as a complement source is shown. For (A,B) , data represent the mean survival (±1 standard deviation) for triplicate samples, as a percentage of bacteria in the absence of antibody (the no antibody control (white) set at 100%, represents 2.0 × 10 3 CFU for SBA and 3.5 × 10 3 CFU for OPA). (C) Blocking of MsrA/B binding to Met(O). Surface plasmon resonance (SPR) of MsrA/B interaction with Met(O) was performed in the presence of pre-immune (light gray) or α-MsrA/B (dark gray) sera. Data represents the mean MsrA/B-Met(O) binding (±1 standard deviation) for triplicate samples, as a percentage of MsrA/B-Met(O) binding in the absence of antibody (the no antibody control (white) set at 100%, represents a K D of 15.4 ± 3.7 nM). For (A–C) , statistically significant differences relative to the no serum controls, using a two-tailed Student's t -test are indicated: * P

    Article Snippet: However, in N. gonorrhoeae the MsrA, MsrB and thioredoxin enzymatic functions are present in a single protein, MsrA/B, that is located in the outer membrane ( ).

    Techniques: Functional Assay, Activity Assay, Standard Deviation, Blocking Assay, Binding Assay, SPR Assay, Two Tailed Test

    Three-way Mauve alignment of N. gonorrhoeae strains NCCP11945 (top), TCDC-NG08107 (middle), and FA1090 (bottom). The numbered blocks represent regions of homology between strains as determined by progressive Mauve alignment on default settings. White vertical lines within blocks represent small localized areas of the genome sequences that have not aligned. The largest of these is the Gonococcal Genetic Island (GGI), which is present in strain NCCP11945 but not the other two strains. Blocks below the central line represent sequences that are inverted in comparison to the strain NCCP11945 arrangement. Homologous blocks are numbered 1 to 14. Blocks 5 and 12 are absent from TCDC-NG08107. Positions of prophage sequences, some of which are fragmented, are indicted: NGOФ1 (Ф1); NGOФ2 (Ф2); NGOФ3 (Ф3); NGOФ4 (Ф4); NGOФ5 (Ф5); NGOФ6 (Ф6); NGOФ7 (Ф7); NGOФ8 (Ф8); and NGOФ9 (Ф9). Labeled are the positions of block 2 flanking IS1106 elements, the block 4 flanking SSREE elements, and the block 11–14 flanking CREE in strain NCCP11945. Note that the chromosomal positions for strain TCDC-NG08107 do not equate to those in GenBank; position 1 was adjusted to be the first base of dnaA to facilitate comparisons.

    Journal: PLoS ONE

    Article Title: Sequence Features Contributing to Chromosomal Rearrangements in Neisseria gonorrhoeae

    doi: 10.1371/journal.pone.0046023

    Figure Lengend Snippet: Three-way Mauve alignment of N. gonorrhoeae strains NCCP11945 (top), TCDC-NG08107 (middle), and FA1090 (bottom). The numbered blocks represent regions of homology between strains as determined by progressive Mauve alignment on default settings. White vertical lines within blocks represent small localized areas of the genome sequences that have not aligned. The largest of these is the Gonococcal Genetic Island (GGI), which is present in strain NCCP11945 but not the other two strains. Blocks below the central line represent sequences that are inverted in comparison to the strain NCCP11945 arrangement. Homologous blocks are numbered 1 to 14. Blocks 5 and 12 are absent from TCDC-NG08107. Positions of prophage sequences, some of which are fragmented, are indicted: NGOФ1 (Ф1); NGOФ2 (Ф2); NGOФ3 (Ф3); NGOФ4 (Ф4); NGOФ5 (Ф5); NGOФ6 (Ф6); NGOФ7 (Ф7); NGOФ8 (Ф8); and NGOФ9 (Ф9). Labeled are the positions of block 2 flanking IS1106 elements, the block 4 flanking SSREE elements, and the block 11–14 flanking CREE in strain NCCP11945. Note that the chromosomal positions for strain TCDC-NG08107 do not equate to those in GenBank; position 1 was adjusted to be the first base of dnaA to facilitate comparisons.

    Article Snippet: The sequence of NGOФ3 is found in two segments in N. gonorrhoeae strain FA1090 , being divided by the insertion of NGOФ9 ( ).

    Techniques: Labeling, Blocking Assay

    Dot plot alignments of the genome sequences of N. gonorrhoeae strains NCCP11945, TCDC-NG08107, and FA1090. Panel A: alignment of the complete genome sequence of strain NCCP11945 (horizontal) against TCDC-NG08107 (vertical); Panel B: alignment of the complete genome sequence of strain NCCP11945 (horizontal) against FA1090 (vertical); Panel C: alignment of the complete genome sequence of strain TCDC-NG08107 (horizontal) against FA1090 (vertical). The Gonococcal Genetic Island (GGI), present in strain NCCP11945, is indicated in panels A and B. For panels B and C, block numbers from Figure 1 are indicated. Reverse slopes indicate inversions of sequence between the two genomes being compared.

    Journal: PLoS ONE

    Article Title: Sequence Features Contributing to Chromosomal Rearrangements in Neisseria gonorrhoeae

    doi: 10.1371/journal.pone.0046023

    Figure Lengend Snippet: Dot plot alignments of the genome sequences of N. gonorrhoeae strains NCCP11945, TCDC-NG08107, and FA1090. Panel A: alignment of the complete genome sequence of strain NCCP11945 (horizontal) against TCDC-NG08107 (vertical); Panel B: alignment of the complete genome sequence of strain NCCP11945 (horizontal) against FA1090 (vertical); Panel C: alignment of the complete genome sequence of strain TCDC-NG08107 (horizontal) against FA1090 (vertical). The Gonococcal Genetic Island (GGI), present in strain NCCP11945, is indicated in panels A and B. For panels B and C, block numbers from Figure 1 are indicated. Reverse slopes indicate inversions of sequence between the two genomes being compared.

    Article Snippet: The sequence of NGOФ3 is found in two segments in N. gonorrhoeae strain FA1090 , being divided by the insertion of NGOФ9 ( ).

    Techniques: Sequencing, Blocking Assay

    Model for the rearrangement of block 2. Block 2 (green) is present between block 1 (yellow) and block 3 (light blue) in N. gonorrhoeae strains NCCP11945 (shown) and TCDC-NG08107. In our model, this region of DNA has been deleted from this location in strain FA1090, making blocks 1 and 3 adjacent ( Figure 1 ). This is shown at the single base level through alignment of sequence to the left and right of the block 2 sequence in strain NCCP11945 (top) with the contiguous sequence of blocks 1 and 3 in strain FA1090 (bottom). The block 2 region of DNA is located elsewhere in the strain FA1090 genome in an inverted orientation ( Figure 1 ). Excision appears to have been via flanking IS1106 elements ( Table S3 ), followed by ISNgo2 element mediated integration using the native ISNgo2 element in block 2 (green arrow; Table S1 ).

    Journal: PLoS ONE

    Article Title: Sequence Features Contributing to Chromosomal Rearrangements in Neisseria gonorrhoeae

    doi: 10.1371/journal.pone.0046023

    Figure Lengend Snippet: Model for the rearrangement of block 2. Block 2 (green) is present between block 1 (yellow) and block 3 (light blue) in N. gonorrhoeae strains NCCP11945 (shown) and TCDC-NG08107. In our model, this region of DNA has been deleted from this location in strain FA1090, making blocks 1 and 3 adjacent ( Figure 1 ). This is shown at the single base level through alignment of sequence to the left and right of the block 2 sequence in strain NCCP11945 (top) with the contiguous sequence of blocks 1 and 3 in strain FA1090 (bottom). The block 2 region of DNA is located elsewhere in the strain FA1090 genome in an inverted orientation ( Figure 1 ). Excision appears to have been via flanking IS1106 elements ( Table S3 ), followed by ISNgo2 element mediated integration using the native ISNgo2 element in block 2 (green arrow; Table S1 ).

    Article Snippet: The sequence of NGOФ3 is found in two segments in N. gonorrhoeae strain FA1090 , being divided by the insertion of NGOФ9 ( ).

    Techniques: Blocking Assay, Sequencing

    Model for the chromosomal rearrangements observed in N. gonorrhoeae strains NCCP11945 and FA1090. Alignments of the genome sequence data shows that the organisation of the genomes of strains NCCP11945 and FA1090 are different ( Figure 1 ). Analysis of this data reveals that the most likely original order of the genome is that shown in the centre of this figure from left to right as blocks 1 (yellow), 2 (green), 3 (light blue), 4 (purple), 7 (lime), 9 (blue), 8 (teal), 6 (orange), 5 (pink), 10 (violet), 11 (red), 12 (too small to be visible), 13 (too small to be visible), and 14 (bright green), circularising back to block 1 (aqua). In strain FA1090 (top), IS1106-mediated homologous recombination and ISNgo2 mediated reintegration have caused a translocation and inversion of block 2 (green arrow), SSREE-mediated homologous recombination has inverted block 4 (purple arrow), and CREE-mediated homologous recombination has inverted blocks 11–14 together (red arrow). In strain NCCP11945 (bottom), ISNgo2 mediated excision and reintegration have caused the translocation of block 7 (lime arrow), block 9 (blue arrow), and block 8 (teal arrow), and the displacement of block 5 (pink arrow).

    Journal: PLoS ONE

    Article Title: Sequence Features Contributing to Chromosomal Rearrangements in Neisseria gonorrhoeae

    doi: 10.1371/journal.pone.0046023

    Figure Lengend Snippet: Model for the chromosomal rearrangements observed in N. gonorrhoeae strains NCCP11945 and FA1090. Alignments of the genome sequence data shows that the organisation of the genomes of strains NCCP11945 and FA1090 are different ( Figure 1 ). Analysis of this data reveals that the most likely original order of the genome is that shown in the centre of this figure from left to right as blocks 1 (yellow), 2 (green), 3 (light blue), 4 (purple), 7 (lime), 9 (blue), 8 (teal), 6 (orange), 5 (pink), 10 (violet), 11 (red), 12 (too small to be visible), 13 (too small to be visible), and 14 (bright green), circularising back to block 1 (aqua). In strain FA1090 (top), IS1106-mediated homologous recombination and ISNgo2 mediated reintegration have caused a translocation and inversion of block 2 (green arrow), SSREE-mediated homologous recombination has inverted block 4 (purple arrow), and CREE-mediated homologous recombination has inverted blocks 11–14 together (red arrow). In strain NCCP11945 (bottom), ISNgo2 mediated excision and reintegration have caused the translocation of block 7 (lime arrow), block 9 (blue arrow), and block 8 (teal arrow), and the displacement of block 5 (pink arrow).

    Article Snippet: The sequence of NGOФ3 is found in two segments in N. gonorrhoeae strain FA1090 , being divided by the insertion of NGOФ9 ( ).

    Techniques: Sequencing, Blocking Assay, Homologous Recombination, Translocation Assay

    Summary of the number and percentage of TRNG isolates obtained in Korea by year. TRNG, high-level tetracycline-resistant Neisseria gonorrhoeae .

    Journal: Yonsei Medical Journal

    Article Title: Increasing Incidence of High-Level Tetracycline-Resistant Neisseria gonorrhoeae due to Clonal Spread and Foreign Import

    doi: 10.3349/ymj.2016.57.2.350

    Figure Lengend Snippet: Summary of the number and percentage of TRNG isolates obtained in Korea by year. TRNG, high-level tetracycline-resistant Neisseria gonorrhoeae .

    Article Snippet: The antimicrobial susceptibilities of 601 isolates of N. gonorrhoeae from 2004 to 2011 were tested by standard Clinical and Laboratory Standards Institute methods.

    Techniques:

    Frequency distribution of gepotidacin MICs against baseline urogenital N. gonorrhoeae isolates ( N = 69).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Microbiological Analysis from a Phase 2 Randomized Study in Adults Evaluating Single Oral Doses of Gepotidacin in the Treatment of Uncomplicated Urogenital Gonorrhea Caused by Neisseria gonorrhoeae

    doi: 10.1128/AAC.01221-18

    Figure Lengend Snippet: Frequency distribution of gepotidacin MICs against baseline urogenital N. gonorrhoeae isolates ( N = 69).

    Article Snippet: A select set of N. gonorrhoeae isolates recovered from participants, including microbiological successes and failures, from the phase 2 clinical trial having the GyrA (S91F and D95A/G) and ParC (D86N) mutations were tested.

    Techniques: