n ethyl n phenylamino 1 Search Results


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    Tocris zd 7288
    Zd 7288, supplied by Tocris, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zd 7288/product/Tocris
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zd 7288 - by Bioz Stars, 2022-12
    88/100 stars
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    90
    Millipore zd 7288
    Normal LTP in the presence of HCN and Kv7 inhibitors. A . Top, stimulation protocol in the presence of <t>ZD-7288.</t> Bottom, time-course (left) and group data (right) of the EPSP slope in the presence of ZD-7288. B . Top, stimulation protocol in the presence of XE-991. Bottom, time-course (left) and group data (right) of the EPSP slope in the presence of XE-991. C . Top, stimulation protocol in the presence of ZD-7288 + XE-991. Bottom, time-course (left) and group data (right) of the EPSP slope in the presence of ZD-7288 + XE-991. Wilcoxon and Mann-Whitney tests were used: ns, p > 0.05; **, p
    Zd 7288, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zd 7288/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zd 7288 - by Bioz Stars, 2022-12
    90/100 stars
      Buy from Supplier

    94
    Cayman Chemical zd 7288
    Hyperpolarization-activated depolarizing current ( I h ) recorded in whole cell voltage-clamp configuration is increased in BRAFV600E-expressing cortical neurons. A : representative traces of currents in response to hyperpolarizing voltage-step protocol. B : I h activation curve from the voltage-step protocol shown in A (tail currents, dashed circle) with maximal activation around −120 mV, half-activation voltage ( V 1/2 ) of −82.79 mV, and slope factor k of 11.58 −1 mV, which are averaged and fit with Boltzmann curve ( n = 18). C : representative traces from a single cell in whole cell voltage-clamp configuration. Application of 50 µM <t>ZD-7288,</t> a known I h inhibitor, in perfusion system for 5 min blocked I h . D : I h peak current measured as shown in A . The significant increase was only found in GLAST-prog. BRAFV600E neurons ( n = 17) compared with their untransfected neighbors ( n = 6; t = 2.117, P = 0.046). E : I h peak current density was increased in GLAST-prog. BRAFV600E neurons ( n = 16) compared with their untransfected neighbors ( n = 6; t 20 = 3.918, P = 0.000852) and NESTIN-prog. eGFP/mRFP ( n = 4; t 14.08 = 5.546, P = 0.000071); it was also significantly increased in NESTIN-prog. BRAFV600E neurons ( n = 4) compared with GLAST-prog. untransfected neighbors ( n = 6; t 8 = 3.275, P = 0.011276) and NESTIN-prog. ; enhanced green fluorescent protein/monomeric red fluorescent protein (eGFP/mRFP; n = 4; t 4.16 = 3.066, P = 0.035546). * P
    Zd 7288, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zd 7288/product/Cayman Chemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zd 7288 - by Bioz Stars, 2022-12
    94/100 stars
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    98
    Tocris hcn channel blocker zd 7288
    Hyperpolarization-activated depolarizing current ( I h ) recorded in whole cell voltage-clamp configuration is increased in BRAFV600E-expressing cortical neurons. A : representative traces of currents in response to hyperpolarizing voltage-step protocol. B : I h activation curve from the voltage-step protocol shown in A (tail currents, dashed circle) with maximal activation around −120 mV, half-activation voltage ( V 1/2 ) of −82.79 mV, and slope factor k of 11.58 −1 mV, which are averaged and fit with Boltzmann curve ( n = 18). C : representative traces from a single cell in whole cell voltage-clamp configuration. Application of 50 µM <t>ZD-7288,</t> a known I h inhibitor, in perfusion system for 5 min blocked I h . D : I h peak current measured as shown in A . The significant increase was only found in GLAST-prog. BRAFV600E neurons ( n = 17) compared with their untransfected neighbors ( n = 6; t = 2.117, P = 0.046). E : I h peak current density was increased in GLAST-prog. BRAFV600E neurons ( n = 16) compared with their untransfected neighbors ( n = 6; t 20 = 3.918, P = 0.000852) and NESTIN-prog. eGFP/mRFP ( n = 4; t 14.08 = 5.546, P = 0.000071); it was also significantly increased in NESTIN-prog. BRAFV600E neurons ( n = 4) compared with GLAST-prog. untransfected neighbors ( n = 6; t 8 = 3.275, P = 0.011276) and NESTIN-prog. ; enhanced green fluorescent protein/monomeric red fluorescent protein (eGFP/mRFP; n = 4; t 4.16 = 3.066, P = 0.035546). * P
    Hcn Channel Blocker Zd 7288, supplied by Tocris, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcn channel blocker zd 7288/product/Tocris
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn channel blocker zd 7288 - by Bioz Stars, 2022-12
    98/100 stars
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    Image Search Results


    Normal LTP in the presence of HCN and Kv7 inhibitors. A . Top, stimulation protocol in the presence of ZD-7288. Bottom, time-course (left) and group data (right) of the EPSP slope in the presence of ZD-7288. B . Top, stimulation protocol in the presence of XE-991. Bottom, time-course (left) and group data (right) of the EPSP slope in the presence of XE-991. C . Top, stimulation protocol in the presence of ZD-7288 + XE-991. Bottom, time-course (left) and group data (right) of the EPSP slope in the presence of ZD-7288 + XE-991. Wilcoxon and Mann-Whitney tests were used: ns, p > 0.05; **, p

    Journal: bioRxiv

    Article Title: Synaptic and intrinsic potentiation in O-LM interneurons is induced by theta patterns of stimulation

    doi: 10.1101/2022.01.19.476905

    Figure Lengend Snippet: Normal LTP in the presence of HCN and Kv7 inhibitors. A . Top, stimulation protocol in the presence of ZD-7288. Bottom, time-course (left) and group data (right) of the EPSP slope in the presence of ZD-7288. B . Top, stimulation protocol in the presence of XE-991. Bottom, time-course (left) and group data (right) of the EPSP slope in the presence of XE-991. C . Top, stimulation protocol in the presence of ZD-7288 + XE-991. Bottom, time-course (left) and group data (right) of the EPSP slope in the presence of ZD-7288 + XE-991. Wilcoxon and Mann-Whitney tests were used: ns, p > 0.05; **, p

    Article Snippet: PiTx was purchased from Sigma-Aldrich; ZD-7288 [4-(N-ethyl-N-phenylamino)-1,2-dimethyl-6-(methylamino) pyrimidinium chloride], XE-991 dihydrochloride (10,10-bis (4-Pyridinylmethyl)-9(10H )-anthracenone dihydrochloride), NASPM trihydrochloride (N -[3-[[4-[(3-Aminopropyl)amino]butyl]amino]propyl]-1-naphthaleneacetamide trihydrochlo-ride), LY367385 (S )-(+)-α-Amino-4-carboxy-2-methylbenzeneacetic acid), U73122, chelerythrine chloride (1,2-Dimethoxy-12-methyl[1,3]benzodioxolo[5,6-c]phenanthridinium chloride) were obtained from Tocris and PIP2 (phosphatidylinositol-4,5-bisphosphate) from Sigma.

    Techniques: MANN-WHITNEY

    Expression of LTP-IE: down-regulation of HCN and Kv7 channels. A . Voltage-clamp measurements of HCN and Kv7 channel-mediated currents before and after 5 Hz. Top, experimental protocol. Middle, reduction of the HCN channel-mediated current evoked by a hyperpolarizing current step from −77 mV to −142 mV. Left, representative traces. Right, group data. Bottom, reduction of the Kv7 channel-mediated M-type current evoked by a hyperpolarization from −32 mV to −62 mV. Left, representative traces. Right, group data. B . Top, experimental protocol in the presence of the HCN channel inhibitor, ZD-7288 (1 μM). Bottom, time-course (left) and group data (right) of normalized AP number before and after 5 Hz stimulation in the presence of ZD-7288. The grey data correspond to the normalized AP number after 5 Hz stimulation obtained in control. Note the significant difference. C . Input/output curves (left) and rheobase (right) before and after 5 Hz stimulation in the presence of ZD-7288. D . Top, experimental protocol in the presence of the Kv7 channel inhibitor, XE-991 (10 μM). Bottom, time-course (left) and group data (right) of normalized AP number before and after 5 Hz stimulation in the presence of XE-991. The grey data correspond to the normalized AP number after 5 Hz stimulation obtained in control. E . Input/output curve (right) and rheobase (left) before and after 5 Hz stimulation in the presence of XE-991. Time-course (left) and group data (right) of normalized AP number before and after 5 Hz stimulation in the presence of XE-991. F . Top, experimental protocol in the presence of ZD-7288 and XE-991. Bottom, time-course (left) and group data (right) of normalized AP number before and after 5 Hz stimulation in the presence of ZD-7288 and XE-991. The grey data correspond to the normalized AP number after 5 Hz stimulation obtained in control. G . Input/output curves (right) and rheobase (left) before and after 5 Hz stimulation in the presence ZD-7288 and XE-991. Wilcoxon and Mann-Whitney tests were used: ns, p > 0.05; *, p

    Journal: bioRxiv

    Article Title: Synaptic and intrinsic potentiation in O-LM interneurons is induced by theta patterns of stimulation

    doi: 10.1101/2022.01.19.476905

    Figure Lengend Snippet: Expression of LTP-IE: down-regulation of HCN and Kv7 channels. A . Voltage-clamp measurements of HCN and Kv7 channel-mediated currents before and after 5 Hz. Top, experimental protocol. Middle, reduction of the HCN channel-mediated current evoked by a hyperpolarizing current step from −77 mV to −142 mV. Left, representative traces. Right, group data. Bottom, reduction of the Kv7 channel-mediated M-type current evoked by a hyperpolarization from −32 mV to −62 mV. Left, representative traces. Right, group data. B . Top, experimental protocol in the presence of the HCN channel inhibitor, ZD-7288 (1 μM). Bottom, time-course (left) and group data (right) of normalized AP number before and after 5 Hz stimulation in the presence of ZD-7288. The grey data correspond to the normalized AP number after 5 Hz stimulation obtained in control. Note the significant difference. C . Input/output curves (left) and rheobase (right) before and after 5 Hz stimulation in the presence of ZD-7288. D . Top, experimental protocol in the presence of the Kv7 channel inhibitor, XE-991 (10 μM). Bottom, time-course (left) and group data (right) of normalized AP number before and after 5 Hz stimulation in the presence of XE-991. The grey data correspond to the normalized AP number after 5 Hz stimulation obtained in control. E . Input/output curve (right) and rheobase (left) before and after 5 Hz stimulation in the presence of XE-991. Time-course (left) and group data (right) of normalized AP number before and after 5 Hz stimulation in the presence of XE-991. F . Top, experimental protocol in the presence of ZD-7288 and XE-991. Bottom, time-course (left) and group data (right) of normalized AP number before and after 5 Hz stimulation in the presence of ZD-7288 and XE-991. The grey data correspond to the normalized AP number after 5 Hz stimulation obtained in control. G . Input/output curves (right) and rheobase (left) before and after 5 Hz stimulation in the presence ZD-7288 and XE-991. Wilcoxon and Mann-Whitney tests were used: ns, p > 0.05; *, p

    Article Snippet: PiTx was purchased from Sigma-Aldrich; ZD-7288 [4-(N-ethyl-N-phenylamino)-1,2-dimethyl-6-(methylamino) pyrimidinium chloride], XE-991 dihydrochloride (10,10-bis (4-Pyridinylmethyl)-9(10H )-anthracenone dihydrochloride), NASPM trihydrochloride (N -[3-[[4-[(3-Aminopropyl)amino]butyl]amino]propyl]-1-naphthaleneacetamide trihydrochlo-ride), LY367385 (S )-(+)-α-Amino-4-carboxy-2-methylbenzeneacetic acid), U73122, chelerythrine chloride (1,2-Dimethoxy-12-methyl[1,3]benzodioxolo[5,6-c]phenanthridinium chloride) were obtained from Tocris and PIP2 (phosphatidylinositol-4,5-bisphosphate) from Sigma.

    Techniques: Expressing, MANN-WHITNEY

    Hyperpolarization-activated depolarizing current ( I h ) recorded in whole cell voltage-clamp configuration is increased in BRAFV600E-expressing cortical neurons. A : representative traces of currents in response to hyperpolarizing voltage-step protocol. B : I h activation curve from the voltage-step protocol shown in A (tail currents, dashed circle) with maximal activation around −120 mV, half-activation voltage ( V 1/2 ) of −82.79 mV, and slope factor k of 11.58 −1 mV, which are averaged and fit with Boltzmann curve ( n = 18). C : representative traces from a single cell in whole cell voltage-clamp configuration. Application of 50 µM ZD-7288, a known I h inhibitor, in perfusion system for 5 min blocked I h . D : I h peak current measured as shown in A . The significant increase was only found in GLAST-prog. BRAFV600E neurons ( n = 17) compared with their untransfected neighbors ( n = 6; t = 2.117, P = 0.046). E : I h peak current density was increased in GLAST-prog. BRAFV600E neurons ( n = 16) compared with their untransfected neighbors ( n = 6; t 20 = 3.918, P = 0.000852) and NESTIN-prog. eGFP/mRFP ( n = 4; t 14.08 = 5.546, P = 0.000071); it was also significantly increased in NESTIN-prog. BRAFV600E neurons ( n = 4) compared with GLAST-prog. untransfected neighbors ( n = 6; t 8 = 3.275, P = 0.011276) and NESTIN-prog. ; enhanced green fluorescent protein/monomeric red fluorescent protein (eGFP/mRFP; n = 4; t 4.16 = 3.066, P = 0.035546). * P

    Journal: Journal of Neurophysiology

    Article Title: BRAFV600E expression in neural progenitors results in a hyperexcitable phenotype in neocortical pyramidal neurons

    doi: 10.1152/jn.00523.2019

    Figure Lengend Snippet: Hyperpolarization-activated depolarizing current ( I h ) recorded in whole cell voltage-clamp configuration is increased in BRAFV600E-expressing cortical neurons. A : representative traces of currents in response to hyperpolarizing voltage-step protocol. B : I h activation curve from the voltage-step protocol shown in A (tail currents, dashed circle) with maximal activation around −120 mV, half-activation voltage ( V 1/2 ) of −82.79 mV, and slope factor k of 11.58 −1 mV, which are averaged and fit with Boltzmann curve ( n = 18). C : representative traces from a single cell in whole cell voltage-clamp configuration. Application of 50 µM ZD-7288, a known I h inhibitor, in perfusion system for 5 min blocked I h . D : I h peak current measured as shown in A . The significant increase was only found in GLAST-prog. BRAFV600E neurons ( n = 17) compared with their untransfected neighbors ( n = 6; t = 2.117, P = 0.046). E : I h peak current density was increased in GLAST-prog. BRAFV600E neurons ( n = 16) compared with their untransfected neighbors ( n = 6; t 20 = 3.918, P = 0.000852) and NESTIN-prog. eGFP/mRFP ( n = 4; t 14.08 = 5.546, P = 0.000071); it was also significantly increased in NESTIN-prog. BRAFV600E neurons ( n = 4) compared with GLAST-prog. untransfected neighbors ( n = 6; t 8 = 3.275, P = 0.011276) and NESTIN-prog. ; enhanced green fluorescent protein/monomeric red fluorescent protein (eGFP/mRFP; n = 4; t 4.16 = 3.066, P = 0.035546). * P

    Article Snippet: SR-95531 dissolved in ddw to 25 mM stock (gabazine, 10 µM; Abcam, Cambridge, MA; catalog no. ab120042, lot no. GR69200) was used to block GABAA receptor (GABAA R); TTX-citrate dissolved in ddw to 10 mM (1 µM; Abcam, Cambridge, MA; catalog no. ab120055, lot no. ) was used to block sodium currents; 4-aminopyridine (4-AP) dissolved in ddw to 200 mM (30 µM–3 mM; MilliporeSigma; Burlington, MA; catalog no. 275875) was used to block fast-activating, fast-inactivating potassium currents; tetraethylammonium chloride (TEA; 25–40 mM; Abcam, Cambridge, MA; catalog no. ab120275, lot no. GR69136) was used to block sustained potassium currents; ZD-7288 dissolved in ddw (50 µM; Cayman Chemicals, Ann Arbor, MI; catalog no. 1522820000040, batch no. 0476856-6) and ivabradine hydrochloride dissolved in ddw to 100 mM (10 µM; TOCRIS; catalog no. 6542) were used to block hyperpolarization-activated depolarizing currents ( I h).

    Techniques: Expressing, Activation Assay