Journal: Naunyn-Schmiedeberg's Archives of Pharmacology
Article Title: The effect of adenosine deaminase inhibition on the A1 adenosinergic and M2 muscarinergic control of contractility in eu- and hyperthyroid guinea pig atria
Figure Lengend Snippet: Conspectus of protocols and groups. The pivot of all protocols was the construction of two E / c curves with an in vitro treatment inserted into them. The inner and outer annuli of the pie chart represent the E / c curves (showing the agonist used), and the medium annulus symbolizes the in vitro treatment (indicating the applied chemicals in italic ). Sectors of the pie chart denote the particular protocols that progressed from the inside out. The names of the experimental groups, which underwent the protocols, are listed outside of the sectors. Two groups belong to one protocol (one sector): one group including solvent-treated atria and another one involving T 4 -treated atria. Ado adenosine, CPA N 6 -cyclopentyladenosine, MC methacholine, S solvent treatment, T T 4 (thyroxine) treatment, Co control, Krebs Krebs solution, CPX 8-cyclopentyl-1,3-dipropylxanthine, DMSO dimethyl-sulfoxide, NBTI nitrobenzylthioinosine, DCF 2′-deoxycoformycin
Article Snippet: Materials The following chemicals were used: l -thyroxine sodium salt pentahydrate (T4 ), adenosine (non-selective adenosine receptor full agonist), acetyl-β-methylcholine chloride (methacholine: MC; non-selective muscarinic receptor full agonist with high affinity for the M2 muscarinic receptor (M2 receptor)), N6 -cyclopentyladenosine (CPA; selective A1 adenosine receptor full agonist), 8-cyclopentyl-1,3-dipropylxanthine (CPX; selective, competitive A1 adenosine receptor antagonist), and S -(2-hydroxy-5-nitrobenzyl)-6-thioinosine (NBTI; selective inhibitor of nucleoside transporter type ENT1) from Sigma (St. Louis, MO, USA) and pentostatin (2′-deoxycoformycin: DCF; selective inhibitor of adenosine deaminase) in Nipent™ , which was the generous gift of Wyeth Pharmaceuticals (Collegeville, PA, USA).
Techniques: In Vitro