mycotoxin concentrations Search Results


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  • 93
    ATCC a parasiticus atcc 15517
    A Parasiticus Atcc 15517, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore afm1
    Additive study by adding different concentrations of <t>AFM1</t> (20, 50 and 100 µg kg −1 ) in ( a ) whole milk, ( b ) skimmed milk and ( c ) juice milk. The obtained results by the developed aptasensor and commercial ELISA kits are compared (in white) ( n = 3).
    Afm1, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Shimadzu Corporation mycotoxin concentration
    Additive study by adding different concentrations of <t>AFM1</t> (20, 50 and 100 µg kg −1 ) in ( a ) whole milk, ( b ) skimmed milk and ( c ) juice milk. The obtained results by the developed aptasensor and commercial ELISA kits are compared (in white) ( n = 3).
    Mycotoxin Concentration, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Labconco vacuum 172 concentrator
    Additive study by adding different concentrations of <t>AFM1</t> (20, 50 and 100 µg kg −1 ) in ( a ) whole milk, ( b ) skimmed milk and ( c ) juice milk. The obtained results by the developed aptasensor and commercial ELISA kits are compared (in white) ( n = 3).
    Vacuum 172 Concentrator, supplied by Labconco, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore ht 2 toxin
    Selective <t>HT-2</t> toxin detection by STING sensor. After the addition of HT-2 toxin molecules at a final concentration of 1 ng/ml, stepwise blockades were recorded by the probe nanopipette (black line), functionalized with anti HT-2 toxin, whereas essentially no change was observed with the control nanopipette (red line). Applied voltage = - 400 mV vs Ag/AgCl. Solution: PBS (0.01M)/DMF (80/20 v/v). Control nanopipette functionalized with anti-HPV16E6.
    Ht 2 Toxin, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore niv
    Selective <t>HT-2</t> toxin detection by STING sensor. After the addition of HT-2 toxin molecules at a final concentration of 1 ng/ml, stepwise blockades were recorded by the probe nanopipette (black line), functionalized with anti HT-2 toxin, whereas essentially no change was observed with the control nanopipette (red line). Applied voltage = - 400 mV vs Ag/AgCl. Solution: PBS (0.01M)/DMF (80/20 v/v). Control nanopipette functionalized with anti-HPV16E6.
    Niv, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Agilent technologies hplc
    Selective <t>HT-2</t> toxin detection by STING sensor. After the addition of HT-2 toxin molecules at a final concentration of 1 ng/ml, stepwise blockades were recorded by the probe nanopipette (black line), functionalized with anti HT-2 toxin, whereas essentially no change was observed with the control nanopipette (red line). Applied voltage = - 400 mV vs Ag/AgCl. Solution: PBS (0.01M)/DMF (80/20 v/v). Control nanopipette functionalized with anti-HPV16E6.
    Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 11373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore fumonisin b1
    Selective <t>HT-2</t> toxin detection by STING sensor. After the addition of HT-2 toxin molecules at a final concentration of 1 ng/ml, stepwise blockades were recorded by the probe nanopipette (black line), functionalized with anti HT-2 toxin, whereas essentially no change was observed with the control nanopipette (red line). Applied voltage = - 400 mV vs Ag/AgCl. Solution: PBS (0.01M)/DMF (80/20 v/v). Control nanopipette functionalized with anti-HPV16E6.
    Fumonisin B1, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    SouthernBiotech igg1
    CSR is compromised in BRIT1-deficient splenic B cells. ( A ) Immunoblot analysis of BRIT1 and AID in whole-cell lysates of splenic B-cells from Mb1-Cre control (Ctrl) and BRIT1 KO mice (0 h, naive; 48 h and 96 h, LPS + IL-4 stimulation). ( B ) Representative flow cytometry of CSR to <t>IgG1,</t> IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( C ) Quantification of CSR to IgG1, IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( n = 7; ** P
    Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 4385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore patulin
    CSR is compromised in BRIT1-deficient splenic B cells. ( A ) Immunoblot analysis of BRIT1 and AID in whole-cell lysates of splenic B-cells from Mb1-Cre control (Ctrl) and BRIT1 KO mice (0 h, naive; 48 h and 96 h, LPS + IL-4 stimulation). ( B ) Representative flow cytometry of CSR to <t>IgG1,</t> IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( C ) Quantification of CSR to IgG1, IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( n = 7; ** P
    Patulin, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore afb1 solution
    CSR is compromised in BRIT1-deficient splenic B cells. ( A ) Immunoblot analysis of BRIT1 and AID in whole-cell lysates of splenic B-cells from Mb1-Cre control (Ctrl) and BRIT1 KO mice (0 h, naive; 48 h and 96 h, LPS + IL-4 stimulation). ( B ) Representative flow cytometry of CSR to <t>IgG1,</t> IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( C ) Quantification of CSR to IgG1, IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( n = 7; ** P
    Afb1 Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore bovine serum albumin bsa
    Schematic illustrations of the three different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) (top) and magnetic nanoparticles and screen-printed electrodes-based electrochemical biosensor (MNPs-SPEs sensor) (bottom). ( A ) Anti <t>OTA-HRP</t> MNPs-ELISA, ( B ) <t>OTA-BSA-HRP</t> MNPs-ELISA, and ( C ) OTA-HRP MNPs-ELISA.
    Bovine Serum Albumin Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 32017 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Siemens Healthineers t1 weighted mr images
    Schematic illustrations of the three different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) (top) and magnetic nanoparticles and screen-printed electrodes-based electrochemical biosensor (MNPs-SPEs sensor) (bottom). ( A ) Anti <t>OTA-HRP</t> MNPs-ELISA, ( B ) <t>OTA-BSA-HRP</t> MNPs-ELISA, and ( C ) OTA-HRP MNPs-ELISA.
    T1 Weighted Mr Images, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 90/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Additive study by adding different concentrations of AFM1 (20, 50 and 100 µg kg −1 ) in ( a ) whole milk, ( b ) skimmed milk and ( c ) juice milk. The obtained results by the developed aptasensor and commercial ELISA kits are compared (in white) ( n = 3).

    Journal: Nanomaterials

    Article Title: Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

    doi: 10.3390/nano9010104

    Figure Lengend Snippet: Additive study by adding different concentrations of AFM1 (20, 50 and 100 µg kg −1 ) in ( a ) whole milk, ( b ) skimmed milk and ( c ) juice milk. The obtained results by the developed aptasensor and commercial ELISA kits are compared (in white) ( n = 3).

    Article Snippet: MgCl2 , KCl, tris (hydroxymethyl) aminomethane (Tris), N -hydroxysulfosuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), streptavidin and chemical standards of AFM1, AFM2, AFB1 and Ochratoxin A were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    Selectivity evaluation of the developed aptasensor for the detection of AFM1 (1 ng mL −1 ) against other control mycotoxins of AFB1, AFM2 and Ochratoxin A at the same concentration.

    Journal: Nanomaterials

    Article Title: Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

    doi: 10.3390/nano9010104

    Figure Lengend Snippet: Selectivity evaluation of the developed aptasensor for the detection of AFM1 (1 ng mL −1 ) against other control mycotoxins of AFB1, AFM2 and Ochratoxin A at the same concentration.

    Article Snippet: MgCl2 , KCl, tris (hydroxymethyl) aminomethane (Tris), N -hydroxysulfosuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), streptavidin and chemical standards of AFM1, AFM2, AFB1 and Ochratoxin A were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Concentration Assay

    Schematic representation of sensitive fluorescent detection of AFM1 based on Exo III-assistant signal amplification recycles.

    Journal: Nanomaterials

    Article Title: Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

    doi: 10.3390/nano9010104

    Figure Lengend Snippet: Schematic representation of sensitive fluorescent detection of AFM1 based on Exo III-assistant signal amplification recycles.

    Article Snippet: MgCl2 , KCl, tris (hydroxymethyl) aminomethane (Tris), N -hydroxysulfosuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), streptavidin and chemical standards of AFM1, AFM2, AFB1 and Ochratoxin A were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Amplification

    The optimization of the experiment: ( a ) Different amount of first-step resultant solution was submitted to the Exo III-assisted recycling amplification in the presence of AFM1. ( n = 3); ( b ) The AFM1 activated first-step displacement time; ( c ) The fluorescence spectra changes according to the concentration of AFM1 (from top to bottom: 20 ng mL −1 to 0 ng mL −1 ); ( d ) The calibration curve was achieved under optimized conditions.

    Journal: Nanomaterials

    Article Title: Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

    doi: 10.3390/nano9010104

    Figure Lengend Snippet: The optimization of the experiment: ( a ) Different amount of first-step resultant solution was submitted to the Exo III-assisted recycling amplification in the presence of AFM1. ( n = 3); ( b ) The AFM1 activated first-step displacement time; ( c ) The fluorescence spectra changes according to the concentration of AFM1 (from top to bottom: 20 ng mL −1 to 0 ng mL −1 ); ( d ) The calibration curve was achieved under optimized conditions.

    Article Snippet: MgCl2 , KCl, tris (hydroxymethyl) aminomethane (Tris), N -hydroxysulfosuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), streptavidin and chemical standards of AFM1, AFM2, AFB1 and Ochratoxin A were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Amplification, Fluorescence, Concentration Assay

    ( a ) Sensorgram recorded on aMZI sensors by flowing AFM1 (black) and Ochratoxin (red) through the microfluidic chamber. At t = 0, MES buffer is flowing through the sensors. The toxin was then injected at t = 2.5 min and at t = 25 min. The toxin flow is stopped and the MES buffer was injected again; ( b ) Sensorgram recorded on one aMZI sensor by flowing a 50 nM (black) and 10 nM (red) AFM1 solutions in the microfluidic chamber. The phase shift is proportional to the Aflatoxin M1 concentration. The actual phase shift is different from the one in (a) since a different sensor was used.

    Journal: Biosensors

    Article Title: Asymmetric Mach–Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection

    doi: 10.3390/bios6010001

    Figure Lengend Snippet: ( a ) Sensorgram recorded on aMZI sensors by flowing AFM1 (black) and Ochratoxin (red) through the microfluidic chamber. At t = 0, MES buffer is flowing through the sensors. The toxin was then injected at t = 2.5 min and at t = 25 min. The toxin flow is stopped and the MES buffer was injected again; ( b ) Sensorgram recorded on one aMZI sensor by flowing a 50 nM (black) and 10 nM (red) AFM1 solutions in the microfluidic chamber. The phase shift is proportional to the Aflatoxin M1 concentration. The actual phase shift is different from the one in (a) since a different sensor was used.

    Article Snippet: A rabbit polyclonal anti-AFM1 antibody and an horseradish peroxidase (HRP)-conjugated Aflatoxin M1 (AFM1-HRP) contained in the I’screen Afla M1 milk Elisa kit were purchased from Tecna s.r.l. (Padua, Italy), while Aflatoxin M1 and Ochratoxin were purchased from Sigma-Aldrich s.r.l. (Milan, Italy).

    Techniques: Injection, Flow Cytometry, Concentration Assay

    Optimization of the anti-idiotypic antibody-based biochip assay focusing on capture and detection antibody concentration. Anti-toxin mAbs as well as anti-idiotypic mAbs were spotted as capture antibodies with varying concentrations as indicated for (A) STX, (D) T-2/HT-2, and (G) AFM1. (B,E,H) Influence of the detection antibody concentration in absence of toxin on the signal response for each assay setup is exemplarily shown for electrode positions spotted with the highest capture concentration and Neg.Co. for the (B) STX, (E) T-2/HT-2, (H) AFM1 assay. Chosen concentrations of detection mAb are indicated bold. (C,F,I) Influence of the capture antibody concentration on the signal response for each assay layout applying a fixed concentration of detection mAb in the presence and absence of different concentrations of (C) STX, (F) T-2, and (I) AFM1. Toxin concentrations and applied detection mAb concentration are stated in the figures. Chosen capture mAb concentrations of the most sensitive assay layout are highlighted. Error bars represent standard deviation from two biochips with two target electrode positions ( n = 4).

    Journal: Frontiers in Chemistry

    Article Title: Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins

    doi: 10.3389/fchem.2019.00031

    Figure Lengend Snippet: Optimization of the anti-idiotypic antibody-based biochip assay focusing on capture and detection antibody concentration. Anti-toxin mAbs as well as anti-idiotypic mAbs were spotted as capture antibodies with varying concentrations as indicated for (A) STX, (D) T-2/HT-2, and (G) AFM1. (B,E,H) Influence of the detection antibody concentration in absence of toxin on the signal response for each assay setup is exemplarily shown for electrode positions spotted with the highest capture concentration and Neg.Co. for the (B) STX, (E) T-2/HT-2, (H) AFM1 assay. Chosen concentrations of detection mAb are indicated bold. (C,F,I) Influence of the capture antibody concentration on the signal response for each assay layout applying a fixed concentration of detection mAb in the presence and absence of different concentrations of (C) STX, (F) T-2, and (I) AFM1. Toxin concentrations and applied detection mAb concentration are stated in the figures. Chosen capture mAb concentrations of the most sensitive assay layout are highlighted. Error bars represent standard deviation from two biochips with two target electrode positions ( n = 4).

    Article Snippet: The aflatoxin standards AFM1, AFB1, AFG1, AFB2, and AFG2 were purchased from Sigma-Aldrich GmbH (Taufkirchen, Germany).

    Techniques: Concentration Assay, Sensitive Assay, Standard Deviation

    Validation of the biochip assay for detection of (A) STX, (B) HT-2, and (C) AFM1 in urine. Signals were obtained from blank urine (black squares) and urine spiked with target concentration (red circles) of (A) 40 ng/mL STX, (B) 20 ng/mL HT-2, and (C) 8 ng/mL AFM1. Signal in urine was set in relation to the B0 signal in assay buffer. Solid line corresponds to the threshold value T. Dashed line represents the cut-off factor Fm.

    Journal: Frontiers in Chemistry

    Article Title: Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins

    doi: 10.3389/fchem.2019.00031

    Figure Lengend Snippet: Validation of the biochip assay for detection of (A) STX, (B) HT-2, and (C) AFM1 in urine. Signals were obtained from blank urine (black squares) and urine spiked with target concentration (red circles) of (A) 40 ng/mL STX, (B) 20 ng/mL HT-2, and (C) 8 ng/mL AFM1. Signal in urine was set in relation to the B0 signal in assay buffer. Solid line corresponds to the threshold value T. Dashed line represents the cut-off factor Fm.

    Article Snippet: The aflatoxin standards AFM1, AFB1, AFG1, AFB2, and AFG2 were purchased from Sigma-Aldrich GmbH (Taufkirchen, Germany).

    Techniques: Concentration Assay

    Dose-response curves for the anti-idiotypic antibody-based biochip assays. Measurements with different dilutions of (A) STX, (B) T-2 (black circles) and HT-2 (green squares), and (C) AFM1 were performed in assay buffer. Data presented here were obtained using the optimal antibody pairs described in Table 2 . Error bars correspond to the standard deviation obtained from three biochips with two target electrode positions ( n = 6).

    Journal: Frontiers in Chemistry

    Article Title: Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins

    doi: 10.3389/fchem.2019.00031

    Figure Lengend Snippet: Dose-response curves for the anti-idiotypic antibody-based biochip assays. Measurements with different dilutions of (A) STX, (B) T-2 (black circles) and HT-2 (green squares), and (C) AFM1 were performed in assay buffer. Data presented here were obtained using the optimal antibody pairs described in Table 2 . Error bars correspond to the standard deviation obtained from three biochips with two target electrode positions ( n = 6).

    Article Snippet: The aflatoxin standards AFM1, AFB1, AFG1, AFB2, and AFG2 were purchased from Sigma-Aldrich GmbH (Taufkirchen, Germany).

    Techniques: Standard Deviation

    Selective HT-2 toxin detection by STING sensor. After the addition of HT-2 toxin molecules at a final concentration of 1 ng/ml, stepwise blockades were recorded by the probe nanopipette (black line), functionalized with anti HT-2 toxin, whereas essentially no change was observed with the control nanopipette (red line). Applied voltage = - 400 mV vs Ag/AgCl. Solution: PBS (0.01M)/DMF (80/20 v/v). Control nanopipette functionalized with anti-HPV16E6.

    Journal: Biosensors & bioelectronics

    Article Title: UltraSensitive Mycotoxin Detection by STING Sensors

    doi: 10.1016/j.bios.2010.08.016

    Figure Lengend Snippet: Selective HT-2 toxin detection by STING sensor. After the addition of HT-2 toxin molecules at a final concentration of 1 ng/ml, stepwise blockades were recorded by the probe nanopipette (black line), functionalized with anti HT-2 toxin, whereas essentially no change was observed with the control nanopipette (red line). Applied voltage = - 400 mV vs Ag/AgCl. Solution: PBS (0.01M)/DMF (80/20 v/v). Control nanopipette functionalized with anti-HPV16E6.

    Article Snippet: HT-2 toxin was acquired from Sigma–Aldrich (T4138).

    Techniques: Concentration Assay

    CSR is compromised in BRIT1-deficient splenic B cells. ( A ) Immunoblot analysis of BRIT1 and AID in whole-cell lysates of splenic B-cells from Mb1-Cre control (Ctrl) and BRIT1 KO mice (0 h, naive; 48 h and 96 h, LPS + IL-4 stimulation). ( B ) Representative flow cytometry of CSR to IgG1, IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( C ) Quantification of CSR to IgG1, IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( n = 7; ** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: BRCT-domain protein BRIT1 influences class switch recombination

    doi: 10.1073/pnas.1708211114

    Figure Lengend Snippet: CSR is compromised in BRIT1-deficient splenic B cells. ( A ) Immunoblot analysis of BRIT1 and AID in whole-cell lysates of splenic B-cells from Mb1-Cre control (Ctrl) and BRIT1 KO mice (0 h, naive; 48 h and 96 h, LPS + IL-4 stimulation). ( B ) Representative flow cytometry of CSR to IgG1, IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( C ) Quantification of CSR to IgG1, IgG3, and IgA in splenic B cells from Mb1-Cre Ctrl and BRIT1 KO mice. ( n = 7; ** P

    Article Snippet: The following isotype standards were used to calculate absolute concentration values: IgM (no. 14-4752-81; eBioscience), IgG1 (no. 0102-01; Southern Biotechnology Associates), IgG3 (no. 553486; BD Pharmingen), and IgA (no. 553478; BD Pharmingen).

    Techniques: Mouse Assay, Flow Cytometry, Cytometry

    Depletion of MDC1 exacerbates CSR defect in BRIT1 KO B cells. ( A ) Strategy to deplete MDC1 in ex vivo stimulated splenic B cells. B cells were harvested from control and BRIT1 KO mice, activated with LPS + IL-4, and retrovirally infected with shRNA and a GFP marker (sh3 and sh4). WB, Western blot. ( B ) Representative flow cytometry of CSR to IgG1 from Mb1-Cre control and BRIT1 KO mice with MDC1 knockdown constructs sh3 and sh4. ( C ) Quantification of CSR to IgG1 ( n = 3; ** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: BRCT-domain protein BRIT1 influences class switch recombination

    doi: 10.1073/pnas.1708211114

    Figure Lengend Snippet: Depletion of MDC1 exacerbates CSR defect in BRIT1 KO B cells. ( A ) Strategy to deplete MDC1 in ex vivo stimulated splenic B cells. B cells were harvested from control and BRIT1 KO mice, activated with LPS + IL-4, and retrovirally infected with shRNA and a GFP marker (sh3 and sh4). WB, Western blot. ( B ) Representative flow cytometry of CSR to IgG1 from Mb1-Cre control and BRIT1 KO mice with MDC1 knockdown constructs sh3 and sh4. ( C ) Quantification of CSR to IgG1 ( n = 3; ** P

    Article Snippet: The following isotype standards were used to calculate absolute concentration values: IgM (no. 14-4752-81; eBioscience), IgG1 (no. 0102-01; Southern Biotechnology Associates), IgG3 (no. 553486; BD Pharmingen), and IgA (no. 553478; BD Pharmingen).

    Techniques: Ex Vivo, Mouse Assay, Infection, shRNA, Marker, Western Blot, Flow Cytometry, Cytometry, Construct

    BRIT1 and MDC1 could both contribute to the ATM–γ-H2AX axis of DSB resolution during CSR. ( A ) Representative flow cytometry of CSR to IgG1 in splenic B cells derived from Mb1-Cre control, BRIT1 KO, and AID KO mice treated with ATMi or with DMSO vehicle control. ( B ) Quantification of CSR to IgG1 ( n = 3; ** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: BRCT-domain protein BRIT1 influences class switch recombination

    doi: 10.1073/pnas.1708211114

    Figure Lengend Snippet: BRIT1 and MDC1 could both contribute to the ATM–γ-H2AX axis of DSB resolution during CSR. ( A ) Representative flow cytometry of CSR to IgG1 in splenic B cells derived from Mb1-Cre control, BRIT1 KO, and AID KO mice treated with ATMi or with DMSO vehicle control. ( B ) Quantification of CSR to IgG1 ( n = 3; ** P

    Article Snippet: The following isotype standards were used to calculate absolute concentration values: IgM (no. 14-4752-81; eBioscience), IgG1 (no. 0102-01; Southern Biotechnology Associates), IgG3 (no. 553486; BD Pharmingen), and IgA (no. 553478; BD Pharmingen).

    Techniques: Flow Cytometry, Cytometry, Derivative Assay, Mouse Assay

    BRIT1 is dispensable for immune response in vivo. ( A ) Schematic of NP-CGG immunization protocol. GC, germinal center. ( B ) IgM, IgG1, IgG3, and IgA concentrations in serum, determined by ELISA on day 0. Ctrl, control. ( C ) Flow cytometric analysis of GC B cells (B220 + DAPI − GL7 + Fas + ) in control, BRIT1 KO, and AID KO mice following NP-CGG immunization on day 14 and quantification of GC B cells in immunized mice on day 14 ( n = 3). ( D ) Quantification of IgG1 + GC B cells in immunized mice on day 14 ( n = 3). ( E ) Quantification of NP + GC B cells in immunized mice on day 14 ( n = 3). ( F ) Quantification of IgG1 + NP + GC B cells in immunized mice on day 14 ( n = 3). ( G ) IgM and IgG1 concentrations in serum from Ctrl, BRIT1 KO, and AID KO mice following NP-CGG immunization on day 28, determined by ELISA. ( H ) Detection of low-affinity and high-affinity NP-specific IgM and IgG1 serum antibodies in Ctrl ( n = 4), BRIT1 KO ( n = 4), and AID KO ( n = 4) mice following NP-CGG immunization on day 28 by ELISA. Conc., concentration.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: BRCT-domain protein BRIT1 influences class switch recombination

    doi: 10.1073/pnas.1708211114

    Figure Lengend Snippet: BRIT1 is dispensable for immune response in vivo. ( A ) Schematic of NP-CGG immunization protocol. GC, germinal center. ( B ) IgM, IgG1, IgG3, and IgA concentrations in serum, determined by ELISA on day 0. Ctrl, control. ( C ) Flow cytometric analysis of GC B cells (B220 + DAPI − GL7 + Fas + ) in control, BRIT1 KO, and AID KO mice following NP-CGG immunization on day 14 and quantification of GC B cells in immunized mice on day 14 ( n = 3). ( D ) Quantification of IgG1 + GC B cells in immunized mice on day 14 ( n = 3). ( E ) Quantification of NP + GC B cells in immunized mice on day 14 ( n = 3). ( F ) Quantification of IgG1 + NP + GC B cells in immunized mice on day 14 ( n = 3). ( G ) IgM and IgG1 concentrations in serum from Ctrl, BRIT1 KO, and AID KO mice following NP-CGG immunization on day 28, determined by ELISA. ( H ) Detection of low-affinity and high-affinity NP-specific IgM and IgG1 serum antibodies in Ctrl ( n = 4), BRIT1 KO ( n = 4), and AID KO ( n = 4) mice following NP-CGG immunization on day 28 by ELISA. Conc., concentration.

    Article Snippet: The following isotype standards were used to calculate absolute concentration values: IgM (no. 14-4752-81; eBioscience), IgG1 (no. 0102-01; Southern Biotechnology Associates), IgG3 (no. 553486; BD Pharmingen), and IgA (no. 553478; BD Pharmingen).

    Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Mouse Assay, Concentration Assay

    BRCT domains of BRIT1 influence CSR. ( A ) Schematic of BRIT1 protein with N-terminal and C-terminal BRCT domains and various deletions. ΔC, C-terminal BRCT domain deletion; ΔN, N-terminal BRCT domain deletion. ( B ) Schematic of retroviral reconstitution of BRIT1 in ex vivo activated splenic B cells. Splenic B cells were harvested from control and BRIT1 KO mice, activated with LPS + IL-4, and retrovirally reconstituted with BRIT1 variants from pMIG vector, which also expresses GFP as a marker. WB, Western blot. ( C ) Representative flow cytometry of CSR to IgG1 in activated splenic BRIT1 KO B cells retrovirally transduced with vector alone (pMIG), BRIT1-FL, BRIT1-ΔN, or BRIT1-ΔC. The vectors express GFP (numbers in parentheses indicate IgG1 + cell in the GFP + gate). Uninfected activated splenic B cells from Mb1-Cre control and BRIT1 KO mice served as negative controls for GFP gating ( n = 3). ( D ) Quantification of the percentage of CSR to IgG1 in splenic B cells from Mb1-Cre control or BRIT1 KO mice retrovirally reconstituted with BRIT1 variants or left uninfected ( n = 3; ** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: BRCT-domain protein BRIT1 influences class switch recombination

    doi: 10.1073/pnas.1708211114

    Figure Lengend Snippet: BRCT domains of BRIT1 influence CSR. ( A ) Schematic of BRIT1 protein with N-terminal and C-terminal BRCT domains and various deletions. ΔC, C-terminal BRCT domain deletion; ΔN, N-terminal BRCT domain deletion. ( B ) Schematic of retroviral reconstitution of BRIT1 in ex vivo activated splenic B cells. Splenic B cells were harvested from control and BRIT1 KO mice, activated with LPS + IL-4, and retrovirally reconstituted with BRIT1 variants from pMIG vector, which also expresses GFP as a marker. WB, Western blot. ( C ) Representative flow cytometry of CSR to IgG1 in activated splenic BRIT1 KO B cells retrovirally transduced with vector alone (pMIG), BRIT1-FL, BRIT1-ΔN, or BRIT1-ΔC. The vectors express GFP (numbers in parentheses indicate IgG1 + cell in the GFP + gate). Uninfected activated splenic B cells from Mb1-Cre control and BRIT1 KO mice served as negative controls for GFP gating ( n = 3). ( D ) Quantification of the percentage of CSR to IgG1 in splenic B cells from Mb1-Cre control or BRIT1 KO mice retrovirally reconstituted with BRIT1 variants or left uninfected ( n = 3; ** P

    Article Snippet: The following isotype standards were used to calculate absolute concentration values: IgM (no. 14-4752-81; eBioscience), IgG1 (no. 0102-01; Southern Biotechnology Associates), IgG3 (no. 553486; BD Pharmingen), and IgA (no. 553478; BD Pharmingen).

    Techniques: Ex Vivo, Mouse Assay, Plasmid Preparation, Marker, Western Blot, Flow Cytometry, Cytometry, Transduction

    ErbB3 is expressed on macrophages and neuregulin (NRG1) is expressed by tumor cells. a BAC macrophages, MDA-MB 231 breast cancer cells (231), and human umbilical vein endothelial cells (HUVEC) were analyzed using fluorescence-activated cell sorting (top) to determine surface ErbB3 expression. Cells were labeled with an ErbB3 blocking antibody reactive to both mouse and human ErbB3, with the corresponding IgG isotype and secondary antibody only as the experimental controls. Quantitation (bottom) is shown as the mean and SEM for the ErbB3 blocking antibody minus the mean signal of the isotype control from three independent experiments: *** p

    Journal: Breast Cancer Research : BCR

    Article Title: A novel neuregulin – jagged1 paracrine loop in breast cancer transendothelial migration

    doi: 10.1186/s13058-018-0960-8

    Figure Lengend Snippet: ErbB3 is expressed on macrophages and neuregulin (NRG1) is expressed by tumor cells. a BAC macrophages, MDA-MB 231 breast cancer cells (231), and human umbilical vein endothelial cells (HUVEC) were analyzed using fluorescence-activated cell sorting (top) to determine surface ErbB3 expression. Cells were labeled with an ErbB3 blocking antibody reactive to both mouse and human ErbB3, with the corresponding IgG isotype and secondary antibody only as the experimental controls. Quantitation (bottom) is shown as the mean and SEM for the ErbB3 blocking antibody minus the mean signal of the isotype control from three independent experiments: *** p

    Article Snippet: Then, either the ErbB3 blocking antibody (cat# MS-303-PABX, Thermo Scientific, Fremont, CA, USA) or mouse IgG1 isotype control (cat# 0102-01 Southern Biotech, Birmingham, AL, USA) were added at a concentration of 10 μg/mL for 30 min, with mixing of the tubes by flicking every 10 min to ensure proper labeling.

    Techniques: BAC Assay, Multiple Displacement Amplification, Fluorescence, FACS, Expressing, Labeling, Blocking Assay, Quantitation Assay

    Blocking ErbB3 inhibits macrophage-induced transendothelial migration . a MDA-MB 231 cells were placed in transwells coated with Matrigel and endothelial cells, either alone (231 Alone) or in the presence of macrophages (231 + BAC), and left untreated (No Ab) or with either an IgG isotype control (IgG) or ErbB3 blocking antibody (ErbB3). Transendothelial migration was measured after 24 h. Results are shown as the mean and SEM of the number of tumor cells crossing the endothelial layer per field. N = 4; ** p

    Journal: Breast Cancer Research : BCR

    Article Title: A novel neuregulin – jagged1 paracrine loop in breast cancer transendothelial migration

    doi: 10.1186/s13058-018-0960-8

    Figure Lengend Snippet: Blocking ErbB3 inhibits macrophage-induced transendothelial migration . a MDA-MB 231 cells were placed in transwells coated with Matrigel and endothelial cells, either alone (231 Alone) or in the presence of macrophages (231 + BAC), and left untreated (No Ab) or with either an IgG isotype control (IgG) or ErbB3 blocking antibody (ErbB3). Transendothelial migration was measured after 24 h. Results are shown as the mean and SEM of the number of tumor cells crossing the endothelial layer per field. N = 4; ** p

    Article Snippet: Then, either the ErbB3 blocking antibody (cat# MS-303-PABX, Thermo Scientific, Fremont, CA, USA) or mouse IgG1 isotype control (cat# 0102-01 Southern Biotech, Birmingham, AL, USA) were added at a concentration of 10 μg/mL for 30 min, with mixing of the tubes by flicking every 10 min to ensure proper labeling.

    Techniques: Blocking Assay, Migration, Multiple Displacement Amplification, BAC Assay

    Schematic illustrations of the three different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) (top) and magnetic nanoparticles and screen-printed electrodes-based electrochemical biosensor (MNPs-SPEs sensor) (bottom). ( A ) Anti OTA-HRP MNPs-ELISA, ( B ) OTA-BSA-HRP MNPs-ELISA, and ( C ) OTA-HRP MNPs-ELISA.

    Journal: Toxins

    Article Title: Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

    doi: 10.3390/toxins10080317

    Figure Lengend Snippet: Schematic illustrations of the three different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) (top) and magnetic nanoparticles and screen-printed electrodes-based electrochemical biosensor (MNPs-SPEs sensor) (bottom). ( A ) Anti OTA-HRP MNPs-ELISA, ( B ) OTA-BSA-HRP MNPs-ELISA, and ( C ) OTA-HRP MNPs-ELISA.

    Article Snippet: Materials Analytical standards of mycotoxins, bovine serum albumin (BSA), and peroxidase from horseradish (HRP) were obtained from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    Calibration curves of different types of MNPs-ELISA. The log concentration of ochratoxin A is plotted along the x -axis, while the inhibition rate is on the y -axis. The error bar indicates the standard deviation. ( A ) Anti OTA-HRP MNPs-ELISA, ( B ) OTA-BSA-HRP MNPs-ELISA, and ( C ) OTA-HRP MNPs-ELISA.

    Journal: Toxins

    Article Title: Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

    doi: 10.3390/toxins10080317

    Figure Lengend Snippet: Calibration curves of different types of MNPs-ELISA. The log concentration of ochratoxin A is plotted along the x -axis, while the inhibition rate is on the y -axis. The error bar indicates the standard deviation. ( A ) Anti OTA-HRP MNPs-ELISA, ( B ) OTA-BSA-HRP MNPs-ELISA, and ( C ) OTA-HRP MNPs-ELISA.

    Article Snippet: Materials Analytical standards of mycotoxins, bovine serum albumin (BSA), and peroxidase from horseradish (HRP) were obtained from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Inhibition, Standard Deviation