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  • 99
    Millipore e coli murb
    E Coli Murb, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli murb/product/Millipore
    Average 99 stars, based on 4 article reviews
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    e coli murb - by Bioz Stars, 2020-09
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    92
    3M Co murb based buffer
    Murb Based Buffer, supplied by 3M Co, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 12 article reviews
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    murb based buffer - by Bioz Stars, 2020-09
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    90
    Brookhaven Instruments staphylococcus aureus murb
    Staphylococcus Aureus Murb, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/staphylococcus aureus murb/product/Brookhaven Instruments
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    staphylococcus aureus murb - by Bioz Stars, 2020-09
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    99
    Millipore murb proteins
    <t>Immunodetection</t> of MURA and <t>MURB</t> Proteins. (A) The alternatively spliced transcripts and calculated molecular masses of the respective protein products (in kD) are shown above the MuDR diagram. The polypeptide domains used for generation of antibodies are indicated below the diagram. (B) Specificity of purified antibodies against the GST tag alone (5 μg) and yeast protein extracts (10 μg) expressing His-tagged full-length MURA and MURB proteins that were used for affinity purification of antibodies. M, molecular mass markers (in kD). (C) Detection of MURA in mature embryos and pollen of various maize lines by immunoblot analysis. The sample array contains non-Mutator lines (hybrid A188/B73 and inbred W23), the a1-mum2 reporter line with no copies of MuDR , an epigenetically silenced bz2-mu1 high-copy MuDR . As a result of pollen sterility, the pollen extract was prepared from a young tassel of the Robertson's line (asterisk). (D) Immunodetection of MURB using different antibodies. The root samples were separated on higher percentage gels to demonstrate that the predominant MURB band consists of two distinct polypeptides. Other annotations are as in (C) . (E) Immunoanalysis to demonstrate the presence of MURB and MURA polypeptides in other maize lines, including those reported previously to have no MURB protein, and in other plant species. (F) Accumulation of MURA and MURB proteins in developmentally staged tissues of non-Mutator inbred line W23 and the original Robertson's purple Mutator line.
    Murb Proteins, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/murb proteins/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    murb proteins - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Bristol Myers murb inhibitors
    <t>Immunodetection</t> of MURA and <t>MURB</t> Proteins. (A) The alternatively spliced transcripts and calculated molecular masses of the respective protein products (in kD) are shown above the MuDR diagram. The polypeptide domains used for generation of antibodies are indicated below the diagram. (B) Specificity of purified antibodies against the GST tag alone (5 μg) and yeast protein extracts (10 μg) expressing His-tagged full-length MURA and MURB proteins that were used for affinity purification of antibodies. M, molecular mass markers (in kD). (C) Detection of MURA in mature embryos and pollen of various maize lines by immunoblot analysis. The sample array contains non-Mutator lines (hybrid A188/B73 and inbred W23), the a1-mum2 reporter line with no copies of MuDR , an epigenetically silenced bz2-mu1 high-copy MuDR . As a result of pollen sterility, the pollen extract was prepared from a young tassel of the Robertson's line (asterisk). (D) Immunodetection of MURB using different antibodies. The root samples were separated on higher percentage gels to demonstrate that the predominant MURB band consists of two distinct polypeptides. Other annotations are as in (C) . (E) Immunoanalysis to demonstrate the presence of MURB and MURA polypeptides in other maize lines, including those reported previously to have no MURB protein, and in other plant species. (F) Accumulation of MURA and MURB proteins in developmentally staged tissues of non-Mutator inbred line W23 and the original Robertson's purple Mutator line.
    Murb Inhibitors, supplied by Bristol Myers, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/murb inhibitors/product/Bristol Myers
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    murb inhibitors - by Bioz Stars, 2020-09
    85/100 stars
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    Image Search Results


    Immunodetection of MURA and MURB Proteins. (A) The alternatively spliced transcripts and calculated molecular masses of the respective protein products (in kD) are shown above the MuDR diagram. The polypeptide domains used for generation of antibodies are indicated below the diagram. (B) Specificity of purified antibodies against the GST tag alone (5 μg) and yeast protein extracts (10 μg) expressing His-tagged full-length MURA and MURB proteins that were used for affinity purification of antibodies. M, molecular mass markers (in kD). (C) Detection of MURA in mature embryos and pollen of various maize lines by immunoblot analysis. The sample array contains non-Mutator lines (hybrid A188/B73 and inbred W23), the a1-mum2 reporter line with no copies of MuDR , an epigenetically silenced bz2-mu1 high-copy MuDR . As a result of pollen sterility, the pollen extract was prepared from a young tassel of the Robertson's line (asterisk). (D) Immunodetection of MURB using different antibodies. The root samples were separated on higher percentage gels to demonstrate that the predominant MURB band consists of two distinct polypeptides. Other annotations are as in (C) . (E) Immunoanalysis to demonstrate the presence of MURB and MURA polypeptides in other maize lines, including those reported previously to have no MURB protein, and in other plant species. (F) Accumulation of MURA and MURB proteins in developmentally staged tissues of non-Mutator inbred line W23 and the original Robertson's purple Mutator line.

    Journal: The Plant Cell

    Article Title: Expression and Post-Transcriptional Regulation of Maize Transposable Element MuDR and Its Derivatives

    doi:

    Figure Lengend Snippet: Immunodetection of MURA and MURB Proteins. (A) The alternatively spliced transcripts and calculated molecular masses of the respective protein products (in kD) are shown above the MuDR diagram. The polypeptide domains used for generation of antibodies are indicated below the diagram. (B) Specificity of purified antibodies against the GST tag alone (5 μg) and yeast protein extracts (10 μg) expressing His-tagged full-length MURA and MURB proteins that were used for affinity purification of antibodies. M, molecular mass markers (in kD). (C) Detection of MURA in mature embryos and pollen of various maize lines by immunoblot analysis. The sample array contains non-Mutator lines (hybrid A188/B73 and inbred W23), the a1-mum2 reporter line with no copies of MuDR , an epigenetically silenced bz2-mu1 high-copy MuDR . As a result of pollen sterility, the pollen extract was prepared from a young tassel of the Robertson's line (asterisk). (D) Immunodetection of MURB using different antibodies. The root samples were separated on higher percentage gels to demonstrate that the predominant MURB band consists of two distinct polypeptides. Other annotations are as in (C) . (E) Immunoanalysis to demonstrate the presence of MURB and MURA polypeptides in other maize lines, including those reported previously to have no MURB protein, and in other plant species. (F) Accumulation of MURA and MURB proteins in developmentally staged tissues of non-Mutator inbred line W23 and the original Robertson's purple Mutator line.

    Article Snippet: Immunodetection of MURA and MURB proteins was performed using appropriate dilutions of primary antibodies, alkaline phosphatase–conjugated secondary antibodies (Sigma, St. Louis, MO), and a chemiluminescent substrate CSPD disodium 3-(4-methoxyspiro[1,2-dioxetane-3,2′-(5′-chloro)tricyclo(3.3.1.13,7 )decan]-4-yl)phenyl phosphate (Tropix, Bedford, MA).

    Techniques: Immunodetection, Purification, Expressing, Affinity Purification, Sterility