Journal: Applications in Plant Sciences
Article Title: An efficient technique for primer development and application that integrates fluorescent labeling and multiplex PCR 1
Figure Lengend Snippet: (see p. 4).The effect of multiplex PCR thermocycler programs during the dual labeling process on the amplitude and signal strength of fragment peaks. Shown are electropherograms from microsatellite loci KA16, ch0203b, and ch01h01 in the ‘Redspire’ cultivar of Pyrus calleryana when the thermocycler cycling program was that (A) suggested by Schuelke (2000) as two series of cycles or recommended by the QIAGEN Multiplexing Kit for (B) 35 cycles, (C) 40 cycles, (D) 45 cycles, and (E) 50 cycles. For comparison, the same samples are presented (F) following traditional multiplexed PCR with prelabeled primers and (G) when analyzed individually with a single prelabeled primer pair; in both of these cases, the normal QIAGEN-recommended protocol was used with 35 cycles.
Article Snippet: To expand the applicability of the earlier single-primer tagging method so investigators could fluorescently label multiple primers concurrently in the same PCR reaction and analyze multiple loci at once, we used the QIAGEN PCR Multiplexing Kit (catalog no. 206143; QIAGEN, Valencia, California, USA).
Techniques: Multiplex Assay, Polymerase Chain Reaction, Labeling, Multiplexing