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  • 99
    ATCC 9100 mtdna copy number
    9100 Mtdna Copy Number, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mitochondrial dna copy number
    Depletion of mtDNA in AZT- and ddC-treated Tdp1 −/− cells. The relative mitochondria <t>DNA</t> copy numbers of wild-type and Tdp1 − / − <t>DT40</t> cells were determined using quantitative real-time PCR in triplicates or quintuplets post-treatment with the indicated drug concentrations for 24 h. Mitochondrial DNA copy numbers of untreated wild-type and Tdp1 − / − DT40 cells were set as 1. Relative mtDNA copy numbers of treated cells were normalized to the untreated cell in their respective cell type. At least three independent experiments were averaged, and the error bars represent SEM. A two-tailed t -test was carried out ( n ≥ 3), with the P -value for each group shown above the bars.
    Mitochondrial Dna Copy Number, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    STATA Corporation mtdna copy number
    Joint effect of the 7 identified <t>SNPs</t> on <t>mtDNA</t> copy number in each city and combined analysis
    Mtdna Copy Number, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Detroit R&D mtdna copy number
    Capsule increases mitochondrial network volume. (A) Mitochondrial <t>DNA</t> copy number at 6 h following SchuS4 infection (MOI, 50). (B) Mitochondrial DNA copy number at 6 h following capsule treatment (10 μg/ml). (C and D) Western blot of cytochrome c (Cyt-C) (C) and the corresponding densitometry of cytochrome c levels (D) in BMM cell lysates at 6 h following SchuS4 infection (MOI, 50). (E and F) Western blot of cytochrome c (E) and the corresponding densitometry of cytochrome c levels (F) in BMM cell lysates over time following capsule treatment (10 μg/ml). (G) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 6 h after SchuS4 infection (MOI, 50). (H) The corresponding quantification of the mitochondrial network volume from panel G. Mito/Cyto, MitoTracker Red volume/cytosolic volume ratio. (I) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 24 h after treatment with capsule (10 μg/ml). (J) The corresponding quantification of the mitochondrial network volume from panel I. Western blot images and densitometry results are representative of those from 3 separate experiments. Confocal images are representative of those from 2 separate experiments in which 10 to 12 cells were used per group for 3D reconstruction and quantification of the mitochondrial network volume. For quantification of the mitochondrial volume and <t>mtDNA</t> copy number, data are presented as the mean ± SEM (three technical replicates) from one representative experiment which was repeated three times. P values were determined using Student's t test. *, P
    Mtdna Copy Number, supplied by Detroit R&D, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Detroit R&D mouse mitochondrial dna copy number assay kit
    Capsule increases mitochondrial network volume. (A) Mitochondrial <t>DNA</t> copy number at 6 h following SchuS4 infection (MOI, 50). (B) Mitochondrial DNA copy number at 6 h following capsule treatment (10 μg/ml). (C and D) Western blot of cytochrome c (Cyt-C) (C) and the corresponding densitometry of cytochrome c levels (D) in BMM cell lysates at 6 h following SchuS4 infection (MOI, 50). (E and F) Western blot of cytochrome c (E) and the corresponding densitometry of cytochrome c levels (F) in BMM cell lysates over time following capsule treatment (10 μg/ml). (G) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 6 h after SchuS4 infection (MOI, 50). (H) The corresponding quantification of the mitochondrial network volume from panel G. Mito/Cyto, MitoTracker Red volume/cytosolic volume ratio. (I) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 24 h after treatment with capsule (10 μg/ml). (J) The corresponding quantification of the mitochondrial network volume from panel I. Western blot images and densitometry results are representative of those from 3 separate experiments. Confocal images are representative of those from 2 separate experiments in which 10 to 12 cells were used per group for 3D reconstruction and quantification of the mitochondrial network volume. For quantification of the mitochondrial volume and <t>mtDNA</t> copy number, data are presented as the mean ± SEM (three technical replicates) from one representative experiment which was repeated three times. P values were determined using Student's t test. *, P
    Mouse Mitochondrial Dna Copy Number Assay Kit, supplied by Detroit R&D, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson mtdna copy number
    Capsule increases mitochondrial network volume. (A) Mitochondrial <t>DNA</t> copy number at 6 h following SchuS4 infection (MOI, 50). (B) Mitochondrial DNA copy number at 6 h following capsule treatment (10 μg/ml). (C and D) Western blot of cytochrome c (Cyt-C) (C) and the corresponding densitometry of cytochrome c levels (D) in BMM cell lysates at 6 h following SchuS4 infection (MOI, 50). (E and F) Western blot of cytochrome c (E) and the corresponding densitometry of cytochrome c levels (F) in BMM cell lysates over time following capsule treatment (10 μg/ml). (G) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 6 h after SchuS4 infection (MOI, 50). (H) The corresponding quantification of the mitochondrial network volume from panel G. Mito/Cyto, MitoTracker Red volume/cytosolic volume ratio. (I) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 24 h after treatment with capsule (10 μg/ml). (J) The corresponding quantification of the mitochondrial network volume from panel I. Western blot images and densitometry results are representative of those from 3 separate experiments. Confocal images are representative of those from 2 separate experiments in which 10 to 12 cells were used per group for 3D reconstruction and quantification of the mitochondrial network volume. For quantification of the mitochondrial volume and <t>mtDNA</t> copy number, data are presented as the mean ± SEM (three technical replicates) from one representative experiment which was repeated three times. P values were determined using Student's t test. *, P
    Mtdna Copy Number, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Detroit R&D rat mitochondrial dna copy number assay kit
    Capsule increases mitochondrial network volume. (A) Mitochondrial <t>DNA</t> copy number at 6 h following SchuS4 infection (MOI, 50). (B) Mitochondrial DNA copy number at 6 h following capsule treatment (10 μg/ml). (C and D) Western blot of cytochrome c (Cyt-C) (C) and the corresponding densitometry of cytochrome c levels (D) in BMM cell lysates at 6 h following SchuS4 infection (MOI, 50). (E and F) Western blot of cytochrome c (E) and the corresponding densitometry of cytochrome c levels (F) in BMM cell lysates over time following capsule treatment (10 μg/ml). (G) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 6 h after SchuS4 infection (MOI, 50). (H) The corresponding quantification of the mitochondrial network volume from panel G. Mito/Cyto, MitoTracker Red volume/cytosolic volume ratio. (I) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 24 h after treatment with capsule (10 μg/ml). (J) The corresponding quantification of the mitochondrial network volume from panel I. Western blot images and densitometry results are representative of those from 3 separate experiments. Confocal images are representative of those from 2 separate experiments in which 10 to 12 cells were used per group for 3D reconstruction and quantification of the mitochondrial network volume. For quantification of the mitochondrial volume and <t>mtDNA</t> copy number, data are presented as the mean ± SEM (three technical replicates) from one representative experiment which was repeated three times. P values were determined using Student's t test. *, P
    Rat Mitochondrial Dna Copy Number Assay Kit, supplied by Detroit R&D, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    CEM Corporation mtdna copy number
    The effect of TR and <t>AtPAP1</t> TR root extracts of L. sibiricus on mitochondrial DNA copy number in an CCRF-CEM, K562 and A549 cell line measured by real-time quantitative PCR. The data represent means ± SD. *: p
    Mtdna Copy Number, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher custom mtdna taqman primer probe copy number assay
    The effect of TR and <t>AtPAP1</t> TR root extracts of L. sibiricus on mitochondrial DNA copy number in an CCRF-CEM, K562 and A549 cell line measured by real-time quantitative PCR. The data represent means ± SD. *: p
    Custom Mtdna Taqman Primer Probe Copy Number Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen mitochondrial dna copy number dneasy blood tissue kit
    The effect of TR and <t>AtPAP1</t> TR root extracts of L. sibiricus on mitochondrial DNA copy number in an CCRF-CEM, K562 and A549 cell line measured by real-time quantitative PCR. The data represent means ± SD. *: p
    Mitochondrial Dna Copy Number Dneasy Blood Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Detroit R&D mtdna copy number kit mcn3
    The effect of TR and <t>AtPAP1</t> TR root extracts of L. sibiricus on mitochondrial DNA copy number in an CCRF-CEM, K562 and A549 cell line measured by real-time quantitative PCR. The data represent means ± SD. *: p
    Mtdna Copy Number Kit Mcn3, supplied by Detroit R&D, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biogenesis Inc mtdna copy number
    Effect of seven days of <t>PioG</t> + dNs combination treatment in control (CON) cybrids and m.3243A > G (MUT) cybrids. ( A ) <t>MtDNA</t> copy number was increased following PioG + dNs treatment in both CON and MUT cybrids, but was not affected by treatment with either PioG or dNs alone. * p
    Mtdna Copy Number, supplied by Biogenesis Inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mitochondrial dna mtdna copy number genomic dna
    Effect of seven days of <t>PioG</t> + dNs combination treatment in control (CON) cybrids and m.3243A > G (MUT) cybrids. ( A ) <t>MtDNA</t> copy number was increased following PioG + dNs treatment in both CON and MUT cybrids, but was not affected by treatment with either PioG or dNs alone. * p
    Mitochondrial Dna Mtdna Copy Number Genomic Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Detroit R&D human mitochondrial dna copy number assay kit
    Effect of seven days of <t>PioG</t> + dNs combination treatment in control (CON) cybrids and m.3243A > G (MUT) cybrids. ( A ) <t>MtDNA</t> copy number was increased following PioG + dNs treatment in both CON and MUT cybrids, but was not affected by treatment with either PioG or dNs alone. * p
    Human Mitochondrial Dna Copy Number Assay Kit, supplied by Detroit R&D, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ScienCell human mtdna copy number quantification rt qpcr assay kit
    Effect of seven days of <t>PioG</t> + dNs combination treatment in control (CON) cybrids and m.3243A > G (MUT) cybrids. ( A ) <t>MtDNA</t> copy number was increased following PioG + dNs treatment in both CON and MUT cybrids, but was not affected by treatment with either PioG or dNs alone. * p
    Human Mtdna Copy Number Quantification Rt Qpcr Assay Kit, supplied by ScienCell, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Depletion of mtDNA in AZT- and ddC-treated Tdp1 −/− cells. The relative mitochondria DNA copy numbers of wild-type and Tdp1 − / − DT40 cells were determined using quantitative real-time PCR in triplicates or quintuplets post-treatment with the indicated drug concentrations for 24 h. Mitochondrial DNA copy numbers of untreated wild-type and Tdp1 − / − DT40 cells were set as 1. Relative mtDNA copy numbers of treated cells were normalized to the untreated cell in their respective cell type. At least three independent experiments were averaged, and the error bars represent SEM. A two-tailed t -test was carried out ( n ≥ 3), with the P -value for each group shown above the bars.

    Journal: Nucleic Acids Research

    Article Title: TDP1 repairs nuclear and mitochondrial DNA damage induced by chain-terminating anticancer and antiviral nucleoside analogs

    doi: 10.1093/nar/gkt483

    Figure Lengend Snippet: Depletion of mtDNA in AZT- and ddC-treated Tdp1 −/− cells. The relative mitochondria DNA copy numbers of wild-type and Tdp1 − / − DT40 cells were determined using quantitative real-time PCR in triplicates or quintuplets post-treatment with the indicated drug concentrations for 24 h. Mitochondrial DNA copy numbers of untreated wild-type and Tdp1 − / − DT40 cells were set as 1. Relative mtDNA copy numbers of treated cells were normalized to the untreated cell in their respective cell type. At least three independent experiments were averaged, and the error bars represent SEM. A two-tailed t -test was carried out ( n ≥ 3), with the P -value for each group shown above the bars.

    Article Snippet: Measurement of mitochondrial DNA copy number by quantitative real-time polymerase chain reaction DT40 Tdp1 − / − and wild-type cells were treated with indicated AZT or ddC (Sigma-Aldrich, St. Louis, MO, USA) concentrations, supplemented with 50 μM of uridine and incubated at 37°C for 24 h. The genomic DNA was extracted with Qiagen Dneasy Blood and Tissue Kit (Qiagen, Valencia, CA, USA) and used as templates in quantitative real-time polymerase chain reaction (PCR) with Power SYBR Green PCR Master Mix (Life Technologies, Carlsbad, CA, USA) in triplicates or quintuplets on an ABI 7900 thermocycler (Life Technologies, Carlsbad, CA, USA).

    Techniques: Real-time Polymerase Chain Reaction, Two Tailed Test

    Tdp1 −/− DT40 cells treated with CTNAs show enhanced perturbations in cell cycle profiles and greater DNA damage. ( A ) Wild-type and Tdp1 − / − DT40 cells were treated with 5 μM ACV or 150 nM Ara-C for 24 h before cell cycle analysis. ( B ) Western blots of wild-type and Tdp1 − / − DT40 cells treated with 2.5 μM ACV or 200 nM Ara-C probed for γH2AX are shown. β′-actin served as loading control, and the length of continuous drug treatment is indicated above each lane.

    Journal: Nucleic Acids Research

    Article Title: TDP1 repairs nuclear and mitochondrial DNA damage induced by chain-terminating anticancer and antiviral nucleoside analogs

    doi: 10.1093/nar/gkt483

    Figure Lengend Snippet: Tdp1 −/− DT40 cells treated with CTNAs show enhanced perturbations in cell cycle profiles and greater DNA damage. ( A ) Wild-type and Tdp1 − / − DT40 cells were treated with 5 μM ACV or 150 nM Ara-C for 24 h before cell cycle analysis. ( B ) Western blots of wild-type and Tdp1 − / − DT40 cells treated with 2.5 μM ACV or 200 nM Ara-C probed for γH2AX are shown. β′-actin served as loading control, and the length of continuous drug treatment is indicated above each lane.

    Article Snippet: Measurement of mitochondrial DNA copy number by quantitative real-time polymerase chain reaction DT40 Tdp1 − / − and wild-type cells were treated with indicated AZT or ddC (Sigma-Aldrich, St. Louis, MO, USA) concentrations, supplemented with 50 μM of uridine and incubated at 37°C for 24 h. The genomic DNA was extracted with Qiagen Dneasy Blood and Tissue Kit (Qiagen, Valencia, CA, USA) and used as templates in quantitative real-time polymerase chain reaction (PCR) with Power SYBR Green PCR Master Mix (Life Technologies, Carlsbad, CA, USA) in triplicates or quintuplets on an ABI 7900 thermocycler (Life Technologies, Carlsbad, CA, USA).

    Techniques: Acetylene Reduction Assay, Cell Cycle Assay, Western Blot

    Joint effect of the 7 identified SNPs on mtDNA copy number in each city and combined analysis

    Journal: BMC Genomics

    Article Title: Genetic variants in nuclear DNA along with environmental factors modify mitochondrial DNA copy number: a population-based exome-wide association study

    doi: 10.1186/s12864-018-5142-7

    Figure Lengend Snippet: Joint effect of the 7 identified SNPs on mtDNA copy number in each city and combined analysis

    Article Snippet: Functional annotations for our identified 7 significant SNPs. (DOCX 21 kb) Figure S2. (a) Association between rs9507174 genotypes and mtDNA copy number; (b): Association between rs9507174 genotypes and the expression of MIPEP gene according GTEx database; (c): Association between rs9507174 genotypes and lung cancer risk using our previous data.

    Techniques:

    Capsule increases mitochondrial network volume. (A) Mitochondrial DNA copy number at 6 h following SchuS4 infection (MOI, 50). (B) Mitochondrial DNA copy number at 6 h following capsule treatment (10 μg/ml). (C and D) Western blot of cytochrome c (Cyt-C) (C) and the corresponding densitometry of cytochrome c levels (D) in BMM cell lysates at 6 h following SchuS4 infection (MOI, 50). (E and F) Western blot of cytochrome c (E) and the corresponding densitometry of cytochrome c levels (F) in BMM cell lysates over time following capsule treatment (10 μg/ml). (G) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 6 h after SchuS4 infection (MOI, 50). (H) The corresponding quantification of the mitochondrial network volume from panel G. Mito/Cyto, MitoTracker Red volume/cytosolic volume ratio. (I) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 24 h after treatment with capsule (10 μg/ml). (J) The corresponding quantification of the mitochondrial network volume from panel I. Western blot images and densitometry results are representative of those from 3 separate experiments. Confocal images are representative of those from 2 separate experiments in which 10 to 12 cells were used per group for 3D reconstruction and quantification of the mitochondrial network volume. For quantification of the mitochondrial volume and mtDNA copy number, data are presented as the mean ± SEM (three technical replicates) from one representative experiment which was repeated three times. P values were determined using Student's t test. *, P

    Journal: Infection and Immunity

    Article Title: Temporal Manipulation of Mitochondrial Function by Virulent Francisella tularensis To Limit Inflammation and Control Cell Death

    doi: 10.1128/IAI.00044-18

    Figure Lengend Snippet: Capsule increases mitochondrial network volume. (A) Mitochondrial DNA copy number at 6 h following SchuS4 infection (MOI, 50). (B) Mitochondrial DNA copy number at 6 h following capsule treatment (10 μg/ml). (C and D) Western blot of cytochrome c (Cyt-C) (C) and the corresponding densitometry of cytochrome c levels (D) in BMM cell lysates at 6 h following SchuS4 infection (MOI, 50). (E and F) Western blot of cytochrome c (E) and the corresponding densitometry of cytochrome c levels (F) in BMM cell lysates over time following capsule treatment (10 μg/ml). (G) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 6 h after SchuS4 infection (MOI, 50). (H) The corresponding quantification of the mitochondrial network volume from panel G. Mito/Cyto, MitoTracker Red volume/cytosolic volume ratio. (I) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 24 h after treatment with capsule (10 μg/ml). (J) The corresponding quantification of the mitochondrial network volume from panel I. Western blot images and densitometry results are representative of those from 3 separate experiments. Confocal images are representative of those from 2 separate experiments in which 10 to 12 cells were used per group for 3D reconstruction and quantification of the mitochondrial network volume. For quantification of the mitochondrial volume and mtDNA copy number, data are presented as the mean ± SEM (three technical replicates) from one representative experiment which was repeated three times. P values were determined using Student's t test. *, P

    Article Snippet: The mtDNA copy number was quantified using a mouse mitochondrial DNA copy number assay kit (Detroit R & D, Detroit, MI) per the manufacturer's recommendations.

    Techniques: Infection, Western Blot, Staining

    Capsule increases mitochondrial network volume. (A) Mitochondrial DNA copy number at 6 h following SchuS4 infection (MOI, 50). (B) Mitochondrial DNA copy number at 6 h following capsule treatment (10 μg/ml). (C and D) Western blot of cytochrome c (Cyt-C) (C) and the corresponding densitometry of cytochrome c levels (D) in BMM cell lysates at 6 h following SchuS4 infection (MOI, 50). (E and F) Western blot of cytochrome c (E) and the corresponding densitometry of cytochrome c levels (F) in BMM cell lysates over time following capsule treatment (10 μg/ml). (G) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 6 h after SchuS4 infection (MOI, 50). (H) The corresponding quantification of the mitochondrial network volume from panel G. Mito/Cyto, MitoTracker Red volume/cytosolic volume ratio. (I) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 24 h after treatment with capsule (10 μg/ml). (J) The corresponding quantification of the mitochondrial network volume from panel I. Western blot images and densitometry results are representative of those from 3 separate experiments. Confocal images are representative of those from 2 separate experiments in which 10 to 12 cells were used per group for 3D reconstruction and quantification of the mitochondrial network volume. For quantification of the mitochondrial volume and mtDNA copy number, data are presented as the mean ± SEM (three technical replicates) from one representative experiment which was repeated three times. P values were determined using Student's t test. *, P

    Journal: Infection and Immunity

    Article Title: Temporal Manipulation of Mitochondrial Function by Virulent Francisella tularensis To Limit Inflammation and Control Cell Death

    doi: 10.1128/IAI.00044-18

    Figure Lengend Snippet: Capsule increases mitochondrial network volume. (A) Mitochondrial DNA copy number at 6 h following SchuS4 infection (MOI, 50). (B) Mitochondrial DNA copy number at 6 h following capsule treatment (10 μg/ml). (C and D) Western blot of cytochrome c (Cyt-C) (C) and the corresponding densitometry of cytochrome c levels (D) in BMM cell lysates at 6 h following SchuS4 infection (MOI, 50). (E and F) Western blot of cytochrome c (E) and the corresponding densitometry of cytochrome c levels (F) in BMM cell lysates over time following capsule treatment (10 μg/ml). (G) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 6 h after SchuS4 infection (MOI, 50). (H) The corresponding quantification of the mitochondrial network volume from panel G. Mito/Cyto, MitoTracker Red volume/cytosolic volume ratio. (I) MitoTracker Red staining and Imaris 3D reconstruction of the mitochondrial network in BMM at 24 h after treatment with capsule (10 μg/ml). (J) The corresponding quantification of the mitochondrial network volume from panel I. Western blot images and densitometry results are representative of those from 3 separate experiments. Confocal images are representative of those from 2 separate experiments in which 10 to 12 cells were used per group for 3D reconstruction and quantification of the mitochondrial network volume. For quantification of the mitochondrial volume and mtDNA copy number, data are presented as the mean ± SEM (three technical replicates) from one representative experiment which was repeated three times. P values were determined using Student's t test. *, P

    Article Snippet: The mtDNA copy number was quantified using a mouse mitochondrial DNA copy number assay kit (Detroit R & D, Detroit, MI) per the manufacturer's recommendations.

    Techniques: Infection, Western Blot, Staining

    Correlation between the OCR and the mitochondrial DNA (mtDNA) copy number and mitochondrial membrane potential. ( A ) Scatterplot depicting a weak correlation between the OCR and the mtDNA copy number. ( B ) Fluorescent microscopic images of JC-10 staining representing embryos with high-OCR (upper) and low-OCR (lower). Embryo with high-OCR exhibited strong J-aggregate (red) and weak monomeric form (green) signals. The original magnification was 200 ×.

    Journal: International Journal of Molecular Sciences

    Article Title: A Preclinical Evaluation towards the Clinical Application of Oxygen Consumption Measurement by CERMs by a Mouse Chimera Model

    doi: 10.3390/ijms20225650

    Figure Lengend Snippet: Correlation between the OCR and the mitochondrial DNA (mtDNA) copy number and mitochondrial membrane potential. ( A ) Scatterplot depicting a weak correlation between the OCR and the mtDNA copy number. ( B ) Fluorescent microscopic images of JC-10 staining representing embryos with high-OCR (upper) and low-OCR (lower). Embryo with high-OCR exhibited strong J-aggregate (red) and weak monomeric form (green) signals. The original magnification was 200 ×.

    Article Snippet: MtDNA Copy Number Assay The mtDNA copy number assay was performed with a Mouse Mitochondrial DNA Copy Number Kit (Detroit R & D Inc., Detroit, MI, USA), according to the manufacturer’s instruction.

    Techniques: Staining

    The effect of TR and AtPAP1 TR root extracts of L. sibiricus on mitochondrial DNA copy number in an CCRF-CEM, K562 and A549 cell line measured by real-time quantitative PCR. The data represent means ± SD. *: p

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: An In Vitro Estimation of the Cytotoxicity and Genotoxicity of Root Extract from Leonurus sibiricus L. Overexpressing AtPAP1 against Different Cancer Cell Lines

    doi: 10.3390/molecules23082049

    Figure Lengend Snippet: The effect of TR and AtPAP1 TR root extracts of L. sibiricus on mitochondrial DNA copy number in an CCRF-CEM, K562 and A549 cell line measured by real-time quantitative PCR. The data represent means ± SD. *: p

    Article Snippet: Mitochondrial Copy Number Furthermore, no difference was found between treated and untreated cells, or between treated TR and AtPAP1 TR extracts, with regard to changes in mtDNA copy number for all tested cell lines (K-562, CRF-CEM and A549).

    Techniques: Real-time Polymerase Chain Reaction

    The effect of TR and AtPAP1 TR root extracts of L. sibiricus on mitochondrial DNA (mtDNA) lesion frequency per 10 kb DNA in ( A ) ND1 and ( B ) ND5 genes, estimated by SLR-qRT-PCR amplification of total DNA from CCRF-CEM, K562 and A549. The data represent means ± SD. *: p

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: An In Vitro Estimation of the Cytotoxicity and Genotoxicity of Root Extract from Leonurus sibiricus L. Overexpressing AtPAP1 against Different Cancer Cell Lines

    doi: 10.3390/molecules23082049

    Figure Lengend Snippet: The effect of TR and AtPAP1 TR root extracts of L. sibiricus on mitochondrial DNA (mtDNA) lesion frequency per 10 kb DNA in ( A ) ND1 and ( B ) ND5 genes, estimated by SLR-qRT-PCR amplification of total DNA from CCRF-CEM, K562 and A549. The data represent means ± SD. *: p

    Article Snippet: Mitochondrial Copy Number Furthermore, no difference was found between treated and untreated cells, or between treated TR and AtPAP1 TR extracts, with regard to changes in mtDNA copy number for all tested cell lines (K-562, CRF-CEM and A549).

    Techniques: Quantitative RT-PCR, Amplification

    Effect of seven days of PioG + dNs combination treatment in control (CON) cybrids and m.3243A > G (MUT) cybrids. ( A ) MtDNA copy number was increased following PioG + dNs treatment in both CON and MUT cybrids, but was not affected by treatment with either PioG or dNs alone. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Pioglitazone and Deoxyribonucleoside Combination Treatment Increases Mitochondrial Respiratory Capacity in m.3243A > G MELAS Cybrid Cells

    doi: 10.3390/ijms21062139

    Figure Lengend Snippet: Effect of seven days of PioG + dNs combination treatment in control (CON) cybrids and m.3243A > G (MUT) cybrids. ( A ) MtDNA copy number was increased following PioG + dNs treatment in both CON and MUT cybrids, but was not affected by treatment with either PioG or dNs alone. * p

    Article Snippet: As individual PioG or dNs treatment did not affect mtDNA copy number (ipso facto mitochondrial biogenesis), we focused on the PioG + dNs combination treatment for subsequent experiments.

    Techniques:

    Effect of seven days of PioG + dNs combination treatment on gene expression in control (CON) cybrids and m.3243A > G (MUT) cybrids. ( A ) Expression of mtDNA-encoded transcripts was higher in untreated MUT cybrids compared to untreated CON cybrids. PioG + dNs treatment increased expression in CON cybrids, but reduced expression in MUT cybrids. ( B ) Expression of nuclear-encoded genes associated with mitochondrial biogenesis was increased in CON cybrids following PioG + dNs treatment, but was decreased in MUT cybrids. UT = untreated; values shown are mean ± s.d. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Pioglitazone and Deoxyribonucleoside Combination Treatment Increases Mitochondrial Respiratory Capacity in m.3243A > G MELAS Cybrid Cells

    doi: 10.3390/ijms21062139

    Figure Lengend Snippet: Effect of seven days of PioG + dNs combination treatment on gene expression in control (CON) cybrids and m.3243A > G (MUT) cybrids. ( A ) Expression of mtDNA-encoded transcripts was higher in untreated MUT cybrids compared to untreated CON cybrids. PioG + dNs treatment increased expression in CON cybrids, but reduced expression in MUT cybrids. ( B ) Expression of nuclear-encoded genes associated with mitochondrial biogenesis was increased in CON cybrids following PioG + dNs treatment, but was decreased in MUT cybrids. UT = untreated; values shown are mean ± s.d. * p

    Article Snippet: As individual PioG or dNs treatment did not affect mtDNA copy number (ipso facto mitochondrial biogenesis), we focused on the PioG + dNs combination treatment for subsequent experiments.

    Techniques: Expressing