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  • 80
    Millipore msp i
    Msp I, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 7 article reviews
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    94
    TaKaRa msp i
    Normalized DNA distributions of T-RFs in fractions from the 13 C-DCM labeled test (AE/AE-13D) and the unlabeled test (AE/AE-13N). a 337-bp T-RF digested with <t>Hha</t> I. b 398-bp T-RF digested with <t>Msp</t> I
    Msp I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher msp i
    Rarefaction curves indicating diversity of denitrifying bacteria as revealed by RFLP analysis of cloned nitrite reductase gene fragments ( nirK and nirS ) from upland and marsh soil samples. nirK gene fragments were digested with the restriction enzymes <t>Msp</t> I and Hae <t>III,</t> and nirS gene fragments were digested with Msp I and Hha I. Data points represent average values from 26 replicate rarefaction curves, and thin lines are 95% confidence limits of the average values. The thick lines represent fit to an exponential model: y = a × (1 − e − bx ), where x = number of clones screened, y = cumulative number of RFLP patterns, and a and b are constants.
    Msp I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 443 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim msp i
    Results of PRA for mycobacterial reference strains. Primer pair RPO5′ and RPO3′ was used. Amplified <t>DNA</t> was digested with <t>Msp</t> I (A) or Hae III (B) restriction enzyme and run on a 4% Metaphore agarose gel. Lanes: M, DNA size marker (50-bp ladder; positions are indicated on left [in base pairs]); 1, M. gordonae type IV; 2, M. szulgai ; 3, M. kansasii type I; 4, M. gallinarum ; 5, M. avium ; 6, M. scrofulaceum ; 7, M. asiaticum ; 8, M. chelonae ; 9, M. moriokaese ; 10, M. phlei ; 11, M. pulveris ; 12, M. fortuitum type I; 13, M. austroafricanum ; 14, M. smegmatis ; 15, M. marinum .
    Msp I, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    msp i  (Roche)
    92
    Roche msp i
    (A) Methylation-sensitive fingerprints of M1/3A/3B, M3A/3B, and M1 ES cell DNA after enzyme digestion with either Rsa I, Rsa I with <t>Hpa</t> II, or Rsa I with <t>Msp</t> I for 16 h. Bands which appear to be hypomethylated only in lane 2 are indicated by solid arrows (class I). Bands which appear to be hypomethylated in both lanes 2 and 3 are indicated by open arrows (class II). Solid squares, functional Dnmt genes; open squares, inactivated genes. GCP1, -2, and -4 are GC-poor primers. (B) Southern blot analysis of genomic DNA from M1/3A/3B, M3A/3B, and M1 ES cells using isolated AP-PCR fragment CI-f as a probe. (C) Ms-SNuPE analysis for fragment CII-d from M1/3A/3B, M3A/3B, and M1 ES cell DNA. Quantitative methylation analysis of three CpG sites in CII-d was performed. The percent methylation given under each gel represents the average obtained for the three sites by using the following equation with PhosphorImager quantitation results: [C/(C + T)] × 100, where C is methylated and T is unmethylated. CpG sites are labeled; hatched boxes, repetitive sequences; arrows, PCR primers.
    Msp I, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msp i/product/Roche
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    92
    Toyobo msp i
    (A) Methylation-sensitive fingerprints of M1/3A/3B, M3A/3B, and M1 ES cell DNA after enzyme digestion with either Rsa I, Rsa I with <t>Hpa</t> II, or Rsa I with <t>Msp</t> I for 16 h. Bands which appear to be hypomethylated only in lane 2 are indicated by solid arrows (class I). Bands which appear to be hypomethylated in both lanes 2 and 3 are indicated by open arrows (class II). Solid squares, functional Dnmt genes; open squares, inactivated genes. GCP1, -2, and -4 are GC-poor primers. (B) Southern blot analysis of genomic DNA from M1/3A/3B, M3A/3B, and M1 ES cells using isolated AP-PCR fragment CI-f as a probe. (C) Ms-SNuPE analysis for fragment CII-d from M1/3A/3B, M3A/3B, and M1 ES cell DNA. Quantitative methylation analysis of three CpG sites in CII-d was performed. The percent methylation given under each gel represents the average obtained for the three sites by using the following equation with PhosphorImager quantitation results: [C/(C + T)] × 100, where C is methylated and T is unmethylated. CpG sites are labeled; hatched boxes, repetitive sequences; arrows, PCR primers.
    Msp I, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Kaneka Corp msp i
    (A) Methylation-sensitive fingerprints of M1/3A/3B, M3A/3B, and M1 ES cell DNA after enzyme digestion with either Rsa I, Rsa I with <t>Hpa</t> II, or Rsa I with <t>Msp</t> I for 16 h. Bands which appear to be hypomethylated only in lane 2 are indicated by solid arrows (class I). Bands which appear to be hypomethylated in both lanes 2 and 3 are indicated by open arrows (class II). Solid squares, functional Dnmt genes; open squares, inactivated genes. GCP1, -2, and -4 are GC-poor primers. (B) Southern blot analysis of genomic DNA from M1/3A/3B, M3A/3B, and M1 ES cells using isolated AP-PCR fragment CI-f as a probe. (C) Ms-SNuPE analysis for fragment CII-d from M1/3A/3B, M3A/3B, and M1 ES cell DNA. Quantitative methylation analysis of three CpG sites in CII-d was performed. The percent methylation given under each gel represents the average obtained for the three sites by using the following equation with PhosphorImager quantitation results: [C/(C + T)] × 100, where C is methylated and T is unmethylated. CpG sites are labeled; hatched boxes, repetitive sequences; arrows, PCR primers.
    Msp I, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msp i/product/Kaneka Corp
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    92
    GE Healthcare msp i
    (A) Methylation-sensitive fingerprints of M1/3A/3B, M3A/3B, and M1 ES cell DNA after enzyme digestion with either Rsa I, Rsa I with <t>Hpa</t> II, or Rsa I with <t>Msp</t> I for 16 h. Bands which appear to be hypomethylated only in lane 2 are indicated by solid arrows (class I). Bands which appear to be hypomethylated in both lanes 2 and 3 are indicated by open arrows (class II). Solid squares, functional Dnmt genes; open squares, inactivated genes. GCP1, -2, and -4 are GC-poor primers. (B) Southern blot analysis of genomic DNA from M1/3A/3B, M3A/3B, and M1 ES cells using isolated AP-PCR fragment CI-f as a probe. (C) Ms-SNuPE analysis for fragment CII-d from M1/3A/3B, M3A/3B, and M1 ES cell DNA. Quantitative methylation analysis of three CpG sites in CII-d was performed. The percent methylation given under each gel represents the average obtained for the three sites by using the following equation with PhosphorImager quantitation results: [C/(C + T)] × 100, where C is methylated and T is unmethylated. CpG sites are labeled; hatched boxes, repetitive sequences; arrows, PCR primers.
    Msp I, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msp i/product/GE Healthcare
    Average 92 stars, based on 38 article reviews
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    92
    BIORON GmbH msp i
    Polymerase chain reaction products of the p16(CDKN2A) gene (C540G) following <t>Msp</t> I enzyme digestion and on a 4% agarose gel. The lane marker shows the 100-bp DNA ladder; lanes 1, 3 and 5 are the C/C genotype (104 and 77 bp); lanes 2 and 6 are the C/G genotype
    Msp I, supplied by BIORON GmbH, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msp i/product/BIORON GmbH
    Average 92 stars, based on 12 article reviews
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    90
    Thermo Fisher msp i digestion
    Polymerase chain reaction products of the p16(CDKN2A) gene (C540G) following <t>Msp</t> I enzyme digestion and on a 4% agarose gel. The lane marker shows the 100-bp DNA ladder; lanes 1, 3 and 5 are the C/C genotype (104 and 77 bp); lanes 2 and 6 are the C/G genotype
    Msp I Digestion, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    TaKaRa msp i enzyme
    Gel with different <t>Msp</t> I restriction patterns of analysed <t>amoA</t> clones from five sites in March 2007. Lanes A-M represent different restriction patterns of amoA gene; Lane m1 is 50bp ladder DNA (Transgen, China); Lane m2 is Marker 1 (Transgen, China).
    Msp I Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher fastdigest msp i
    Gel with different <t>Msp</t> I restriction patterns of analysed <t>amoA</t> clones from five sites in March 2007. Lanes A-M represent different restriction patterns of amoA gene; Lane m1 is 50bp ladder DNA (Transgen, China); Lane m2 is Marker 1 (Transgen, China).
    Fastdigest Msp I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Merck KGaA msp i enzyme
    Gel with different <t>Msp</t> I restriction patterns of analysed <t>amoA</t> clones from five sites in March 2007. Lanes A-M represent different restriction patterns of amoA gene; Lane m1 is 50bp ladder DNA (Transgen, China); Lane m2 is Marker 1 (Transgen, China).
    Msp I Enzyme, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msp i enzyme/product/Merck KGaA
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    85
    Roche enzyme msp i
    Gel with different <t>Msp</t> I restriction patterns of analysed <t>amoA</t> clones from five sites in March 2007. Lanes A-M represent different restriction patterns of amoA gene; Lane m1 is 50bp ladder DNA (Transgen, China); Lane m2 is Marker 1 (Transgen, China).
    Enzyme Msp I, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher msp i enzyme
    Agarose gel electrophoresis of ITS-rDNA RFLP profiles by <t>Msp</t> I for representative isolates of C. albicans, C. glabrata and C. kefyr Lanes 1, 2, 4, 6, 8, 9, 10, 12 and 13; clinical isolates of C. albicans , Lanes 5,7 and 11; C. glabrata and lane 3; C. kefyr , M; 50 bp DNA marker
    Msp I Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher msp i restriction enzyme
    Agarose gel electrophoresis of ITS-rDNA RFLP profiles by <t>Msp</t> I for representative isolates of C. albicans, C. glabrata and C. kefyr Lanes 1, 2, 4, 6, 8, 9, 10, 12 and 13; clinical isolates of C. albicans , Lanes 5,7 and 11; C. glabrata and lane 3; C. kefyr , M; 50 bp DNA marker
    Msp I Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher restriction enzyme msp i
    Agarose gel electrophoresis of ITS-PCR products of clinical yeast strains after digestion with <t>Msp</t> I . Lane 1: C. guilliermondii , lane 2: C. glabrata , lane 3,4,6,8,9: C. albicans , lane 5: C. tropicalis , lane 7: C. parapsilosis , and lane M: 100 bp Ladder
    Restriction Enzyme Msp I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    FUJIFILM msp i digested pbr322
    Agarose gel electrophoresis of ITS-PCR products of clinical yeast strains after digestion with <t>Msp</t> I . Lane 1: C. guilliermondii , lane 2: C. glabrata , lane 3,4,6,8,9: C. albicans , lane 5: C. tropicalis , lane 7: C. parapsilosis , and lane M: 100 bp Ladder
    Msp I Digested Pbr322, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Normalized DNA distributions of T-RFs in fractions from the 13 C-DCM labeled test (AE/AE-13D) and the unlabeled test (AE/AE-13N). a 337-bp T-RF digested with Hha I. b 398-bp T-RF digested with Msp I

    Journal: Water, Air, and Soil Pollution

    Article Title: Bacterial Degraders of Coexisting Dichloromethane, Benzene, and Toluene, Identified by Stable-Isotope Probing

    doi: 10.1007/s11270-017-3604-1

    Figure Lengend Snippet: Normalized DNA distributions of T-RFs in fractions from the 13 C-DCM labeled test (AE/AE-13D) and the unlabeled test (AE/AE-13N). a 337-bp T-RF digested with Hha I. b 398-bp T-RF digested with Msp I

    Article Snippet: The PCR products were purified with MinElute® PCR Purification Kit (QIAGEN), then independently digested with restriction enzymes: Hha I (Takara Bio) and Msp I (Takara Bio).

    Techniques: Labeling

    Normalized DNA distributions of T-RFs in fractions from the 13 C-benzene labeled test (AE/AE-13B) and unlabeled test (AE/AE-13N). a 352-bp T-RF digested with Hha I. b 154-bp T-RF digested with Msp I

    Journal: Water, Air, and Soil Pollution

    Article Title: Bacterial Degraders of Coexisting Dichloromethane, Benzene, and Toluene, Identified by Stable-Isotope Probing

    doi: 10.1007/s11270-017-3604-1

    Figure Lengend Snippet: Normalized DNA distributions of T-RFs in fractions from the 13 C-benzene labeled test (AE/AE-13B) and unlabeled test (AE/AE-13N). a 352-bp T-RF digested with Hha I. b 154-bp T-RF digested with Msp I

    Article Snippet: The PCR products were purified with MinElute® PCR Purification Kit (QIAGEN), then independently digested with restriction enzymes: Hha I (Takara Bio) and Msp I (Takara Bio).

    Techniques: Labeling

    Gel electrophoresis patterns of PD-1.1 and PD-1.2. A: The amplified fragments of PD-1.1 were digested with MspI: the polymerase chain reaction (PCR) product size was 265 base pairs (bp) which was digested to 180 and 85 bp, the genotype was identified

    Journal: World Journal of Hepatology

    Article Title: Polymorphisms in programmed death-1 gene are not associated with chronic HBV infection in Chinese patients

    doi: 10.4254/wjh.v3.i3.72

    Figure Lengend Snippet: Gel electrophoresis patterns of PD-1.1 and PD-1.2. A: The amplified fragments of PD-1.1 were digested with MspI: the polymerase chain reaction (PCR) product size was 265 base pairs (bp) which was digested to 180 and 85 bp, the genotype was identified

    Article Snippet: The polymerase chain reaction (PCR) product 265 bp was digested with MspI (TaKaRa Biotechnology, Co., Ltd. Dalian, China) according to the manufacturer’s instructions [total reaction volume 20 μL, including PCR product 10 μL, 10× T buffer 2 μL, 0.1% BSA 2 μL, restriction enzyme (10 U/μL) 1μL, H2 O 5 μL] and separated by electrophoresis on 3% agarose gels.

    Techniques: Nucleic Acid Electrophoresis, Amplification, Polymerase Chain Reaction

    Representative fragments in methylation sensitive amplification polymorphism (MSAP) profiles (A,B) and the relationship between U- values and ploidy level (C–E). (A) Hpa II and Msp I sensitivities to 5-CCGG methylation status (“+”: enzyme cuts; “-”: enzyme does not cut): three types of fragment generated. Type I: non-methylated, appearing in both the H and M lanes; Type II: fully methylated, present in the M but not the H lanes; Type III: hemi-methylated, present in the H but not the M lanes; (B) Examples of MSAP electrophoresis patterns in ABI3730xl; The relationship between U- values and ploidy level (C-E) . U- values associated with (C) total methylation, (D) full methylation, (E) hemi-methylation. A higher U -value implies a larger difference between different samples, but only U -values > 1.96 were statistically significant.

    Journal: Frontiers in Plant Science

    Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy

    doi: 10.3389/fpls.2015.00668

    Figure Lengend Snippet: Representative fragments in methylation sensitive amplification polymorphism (MSAP) profiles (A,B) and the relationship between U- values and ploidy level (C–E). (A) Hpa II and Msp I sensitivities to 5-CCGG methylation status (“+”: enzyme cuts; “-”: enzyme does not cut): three types of fragment generated. Type I: non-methylated, appearing in both the H and M lanes; Type II: fully methylated, present in the M but not the H lanes; Type III: hemi-methylated, present in the H but not the M lanes; (B) Examples of MSAP electrophoresis patterns in ABI3730xl; The relationship between U- values and ploidy level (C-E) . U- values associated with (C) total methylation, (D) full methylation, (E) hemi-methylation. A higher U -value implies a larger difference between different samples, but only U -values > 1.96 were statistically significant.

    Article Snippet: A 5 μL aliquot of the product was pre-amplified in the presence of 0.2 μM Eco RI and 0.2 μM Hpa II-Msp I non-selective primers (sequences given in Supplementary Table ) in a 25 μL reaction containing 2.5 μL 10x PCR buffer, 1.5 mM MgCl2 , 0.2 mM dNTP, and 2 U Taq polymerase (Takara, Japan).

    Techniques: Methylation, Amplification, Generated, Electrophoresis

    Rarefaction curves indicating diversity of denitrifying bacteria as revealed by RFLP analysis of cloned nitrite reductase gene fragments ( nirK and nirS ) from upland and marsh soil samples. nirK gene fragments were digested with the restriction enzymes Msp I and Hae III, and nirS gene fragments were digested with Msp I and Hha I. Data points represent average values from 26 replicate rarefaction curves, and thin lines are 95% confidence limits of the average values. The thick lines represent fit to an exponential model: y = a × (1 − e − bx ), where x = number of clones screened, y = cumulative number of RFLP patterns, and a and b are constants.

    Journal: Applied and Environmental Microbiology

    Article Title: Diversity of Nitrite Reductase (nirK and nirS) Gene Fragments in Forested Upland and Wetland Soils

    doi: 10.1128/AEM.68.4.1893-1900.2002

    Figure Lengend Snippet: Rarefaction curves indicating diversity of denitrifying bacteria as revealed by RFLP analysis of cloned nitrite reductase gene fragments ( nirK and nirS ) from upland and marsh soil samples. nirK gene fragments were digested with the restriction enzymes Msp I and Hae III, and nirS gene fragments were digested with Msp I and Hha I. Data points represent average values from 26 replicate rarefaction curves, and thin lines are 95% confidence limits of the average values. The thick lines represent fit to an exponential model: y = a × (1 − e − bx ), where x = number of clones screened, y = cumulative number of RFLP patterns, and a and b are constants.

    Article Snippet: The products from nirK clones were digested in two separate reactions with the restriction enzymes Hae III (New England BioLabs, Beverly, Mass.) and Msp I (Gibco BRL, Rockville, Md.), while nirS clones were digested with Hha I (New England BioLabs) and Msp I.

    Techniques: Clone Assay

    Results of PRA for mycobacterial reference strains. Primer pair RPO5′ and RPO3′ was used. Amplified DNA was digested with Msp I (A) or Hae III (B) restriction enzyme and run on a 4% Metaphore agarose gel. Lanes: M, DNA size marker (50-bp ladder; positions are indicated on left [in base pairs]); 1, M. gordonae type IV; 2, M. szulgai ; 3, M. kansasii type I; 4, M. gallinarum ; 5, M. avium ; 6, M. scrofulaceum ; 7, M. asiaticum ; 8, M. chelonae ; 9, M. moriokaese ; 10, M. phlei ; 11, M. pulveris ; 12, M. fortuitum type I; 13, M. austroafricanum ; 14, M. smegmatis ; 15, M. marinum .

    Journal: Journal of Clinical Microbiology

    Article Title: Species Identification of Mycobacteria by PCR-Restriction Fragment Length Polymorphism of the rpoB Gene

    doi:

    Figure Lengend Snippet: Results of PRA for mycobacterial reference strains. Primer pair RPO5′ and RPO3′ was used. Amplified DNA was digested with Msp I (A) or Hae III (B) restriction enzyme and run on a 4% Metaphore agarose gel. Lanes: M, DNA size marker (50-bp ladder; positions are indicated on left [in base pairs]); 1, M. gordonae type IV; 2, M. szulgai ; 3, M. kansasii type I; 4, M. gallinarum ; 5, M. avium ; 6, M. scrofulaceum ; 7, M. asiaticum ; 8, M. chelonae ; 9, M. moriokaese ; 10, M. phlei ; 11, M. pulveris ; 12, M. fortuitum type I; 13, M. austroafricanum ; 14, M. smegmatis ; 15, M. marinum .

    Article Snippet: Most of the time, 10 to 16 μl of PCR product (approximately 1 to 1.5 μg of DNA) was digested in a 20-μl reaction volume containing 5 U of Msp I (Boehringer Mannheim Biochemicals, Mannheim, Germany) and 2 μl of the 10× reaction buffer supplied by manufacturer.

    Techniques: Amplification, Agarose Gel Electrophoresis, Marker

    (A) Methylation-sensitive fingerprints of M1/3A/3B, M3A/3B, and M1 ES cell DNA after enzyme digestion with either Rsa I, Rsa I with Hpa II, or Rsa I with Msp I for 16 h. Bands which appear to be hypomethylated only in lane 2 are indicated by solid arrows (class I). Bands which appear to be hypomethylated in both lanes 2 and 3 are indicated by open arrows (class II). Solid squares, functional Dnmt genes; open squares, inactivated genes. GCP1, -2, and -4 are GC-poor primers. (B) Southern blot analysis of genomic DNA from M1/3A/3B, M3A/3B, and M1 ES cells using isolated AP-PCR fragment CI-f as a probe. (C) Ms-SNuPE analysis for fragment CII-d from M1/3A/3B, M3A/3B, and M1 ES cell DNA. Quantitative methylation analysis of three CpG sites in CII-d was performed. The percent methylation given under each gel represents the average obtained for the three sites by using the following equation with PhosphorImager quantitation results: [C/(C + T)] × 100, where C is methylated and T is unmethylated. CpG sites are labeled; hatched boxes, repetitive sequences; arrows, PCR primers.

    Journal: Molecular and Cellular Biology

    Article Title: Cooperativity between DNA Methyltransferases in the Maintenance Methylation of Repetitive Elements

    doi: 10.1128/MCB.22.2.480-491.2002

    Figure Lengend Snippet: (A) Methylation-sensitive fingerprints of M1/3A/3B, M3A/3B, and M1 ES cell DNA after enzyme digestion with either Rsa I, Rsa I with Hpa II, or Rsa I with Msp I for 16 h. Bands which appear to be hypomethylated only in lane 2 are indicated by solid arrows (class I). Bands which appear to be hypomethylated in both lanes 2 and 3 are indicated by open arrows (class II). Solid squares, functional Dnmt genes; open squares, inactivated genes. GCP1, -2, and -4 are GC-poor primers. (B) Southern blot analysis of genomic DNA from M1/3A/3B, M3A/3B, and M1 ES cells using isolated AP-PCR fragment CI-f as a probe. (C) Ms-SNuPE analysis for fragment CII-d from M1/3A/3B, M3A/3B, and M1 ES cell DNA. Quantitative methylation analysis of three CpG sites in CII-d was performed. The percent methylation given under each gel represents the average obtained for the three sites by using the following equation with PhosphorImager quantitation results: [C/(C + T)] × 100, where C is methylated and T is unmethylated. CpG sites are labeled; hatched boxes, repetitive sequences; arrows, PCR primers.

    Article Snippet: Ten micrograms of DNA from cell lines was separately digested with either 50 U of Rsa I, 50 U each of Rsa I and Hpa II, or 50 U each of Rsa I and Msp I (Roche) at 37°C for 16 h. Digested genomic DNA was electrophoresed on a 0.7% agarose gel and Southern transferred to Zeta-Probe (Bio-Rad) membranes overnight.

    Techniques: Methylation, Functional Assay, Southern Blot, Isolation, Polymerase Chain Reaction, Mass Spectrometry, Quantitation Assay, Labeling

    Polymerase chain reaction products of the p16(CDKN2A) gene (C540G) following Msp I enzyme digestion and on a 4% agarose gel. The lane marker shows the 100-bp DNA ladder; lanes 1, 3 and 5 are the C/C genotype (104 and 77 bp); lanes 2 and 6 are the C/G genotype

    Journal: Biomedical Reports

    Article Title: Association between p16(CDKN2A) C540G polymorphism and tumor behavior in prolactinoma: A single-center study

    doi: 10.3892/br.2014.281

    Figure Lengend Snippet: Polymerase chain reaction products of the p16(CDKN2A) gene (C540G) following Msp I enzyme digestion and on a 4% agarose gel. The lane marker shows the 100-bp DNA ladder; lanes 1, 3 and 5 are the C/C genotype (104 and 77 bp); lanes 2 and 6 are the C/G genotype

    Article Snippet: The PCR products were digested with Msp I (Bioron GmbH, Ludwigshafen am Rhein, Germany) at 37°C overnight and analyzed on a 4% agarose gel.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker

    Gel with different Msp I restriction patterns of analysed amoA clones from five sites in March 2007. Lanes A-M represent different restriction patterns of amoA gene; Lane m1 is 50bp ladder DNA (Transgen, China); Lane m2 is Marker 1 (Transgen, China).

    Journal: Brazilian Journal of Microbiology

    Article Title: Abundance and diversity of ammonia-oxidizing bacteria in relation to ammonium in a chinese shallow eutrophic urban lake

    doi: 10.1590/S1517-838220100001000031

    Figure Lengend Snippet: Gel with different Msp I restriction patterns of analysed amoA clones from five sites in March 2007. Lanes A-M represent different restriction patterns of amoA gene; Lane m1 is 50bp ladder DNA (Transgen, China); Lane m2 is Marker 1 (Transgen, China).

    Article Snippet: The cloned inserts were reamplified with amoA primers and then digested with the Msp I enzyme (TaKaRa).

    Techniques: Clone Assay, Marker

    Agarose gel electrophoresis of ITS-rDNA RFLP profiles by Msp I for representative isolates of C. albicans, C. glabrata and C. kefyr Lanes 1, 2, 4, 6, 8, 9, 10, 12 and 13; clinical isolates of C. albicans , Lanes 5,7 and 11; C. glabrata and lane 3; C. kefyr , M; 50 bp DNA marker

    Journal: Iranian Journal of Microbiology

    Article Title: Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species

    doi:

    Figure Lengend Snippet: Agarose gel electrophoresis of ITS-rDNA RFLP profiles by Msp I for representative isolates of C. albicans, C. glabrata and C. kefyr Lanes 1, 2, 4, 6, 8, 9, 10, 12 and 13; clinical isolates of C. albicans , Lanes 5,7 and 11; C. glabrata and lane 3; C. kefyr , M; 50 bp DNA marker

    Article Snippet: The obtained products were then subjected to restriction analysis with Msp I enzyme (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Agarose Gel Electrophoresis, Marker

    Agarose gel electrophoresis of ITS-PCR products of clinical yeast strains after digestion with Msp I . Lane 1: C. guilliermondii , lane 2: C. glabrata , lane 3,4,6,8,9: C. albicans , lane 5: C. tropicalis , lane 7: C. parapsilosis , and lane M: 100 bp Ladder

    Journal: Advanced Biomedical Research

    Article Title: Use of restriction fragment length polymorphism to identify Candida species, related to onychomycosis

    doi: 10.4103/2277-9175.156659

    Figure Lengend Snippet: Agarose gel electrophoresis of ITS-PCR products of clinical yeast strains after digestion with Msp I . Lane 1: C. guilliermondii , lane 2: C. glabrata , lane 3,4,6,8,9: C. albicans , lane 5: C. tropicalis , lane 7: C. parapsilosis , and lane M: 100 bp Ladder

    Article Snippet: Digestion with the restriction enzyme MspI and NlaIII PCR products were digested in a final reaction volume of 15 μl containing 3 μl water, 1.5 μl buffer, 1U of restriction enzyme Msp I (Fermentas, Vilnius, Lithuania)[ ] and 10 μl PCR product at 37°C for 2 hours.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction