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  • 94
    New England Biolabs hiscribe t7 arca mrna
    Hiscribe T7 Arca Mrna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher anti reverse cap analog arca
    Anti Reverse Cap Analog Arca, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    New England Biolabs hiscribe t7 arca mrna kit
    Hiscribe T7 Arca Mrna Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiscribe t7 arca mrna kit/product/New England Biolabs
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    90
    Millipore anti msp 3
    Isolated merozoites maintain surface coat integrity. A) E64-treated schizonts were filtered to release free merozoites and haemozoin crystals, and the filtrate was passed over magnetic columns. Merozoite purification was confirmed by Giemsa-stained smears of i) filtrate, ii) retained haemozoin and iii) purified merozoites. B) Merozites were stained with EtBr and enumerated by flow cytometry. C) Washed Merozoites retained surface proteins <t>MSP-3,</t> MSP-6 and AMA-1 by western blot (S: purified Schizonts; M: filtered merozoites; SM: EtBr stained merozoites). D) Merozoite surface proteins are maintained during merozoite isolation and wash steps as shown by surface localisation of MSP-3 and MSP1 19 by immunofluorescence microscopy. Antigens were stained with Alexa-594 and the nucleus with DAPI (panels in order Alexa549; DAPI; brightfield; Alexa549/DAPI; merge).
    Anti Msp 3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen epitect msp kit
    Isolated merozoites maintain surface coat integrity. A) E64-treated schizonts were filtered to release free merozoites and haemozoin crystals, and the filtrate was passed over magnetic columns. Merozoite purification was confirmed by Giemsa-stained smears of i) filtrate, ii) retained haemozoin and iii) purified merozoites. B) Merozites were stained with EtBr and enumerated by flow cytometry. C) Washed Merozoites retained surface proteins <t>MSP-3,</t> MSP-6 and AMA-1 by western blot (S: purified Schizonts; M: filtered merozoites; SM: EtBr stained merozoites). D) Merozoite surface proteins are maintained during merozoite isolation and wash steps as shown by surface localisation of MSP-3 and MSP1 19 by immunofluorescence microscopy. Antigens were stained with Alexa-594 and the nucleus with DAPI (panels in order Alexa549; DAPI; brightfield; Alexa549/DAPI; merge).
    Epitect Msp Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa msp analysis
    Normalized DNA distributions of T-RFs in fractions from the 13 C-DCM labeled test (AE/AE-13D) and the unlabeled test (AE/AE-13N). a 337-bp T-RF digested with <t>Hha</t> I. b 398-bp T-RF digested with <t>Msp</t> I
    Msp Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology msp a
    Elastase deficient Δ lasB mutant is attenuated in the degradation of SP-A during lung infection. (A-C) The amounts of intact <t>mSP-A</t> were not visibly changed at 6-hr (A) or 12- hr (B) post-infection. By 18 hr post-infection (C), intact mSP-A was reduced in the BAL fluid from PAO1- or PDO240lasB-infected SP-A +/+ mice (n = 6), suggesting that mSP-A was degraded in mouse lungs. In contrast, more abundant mSP-A was clearly visible in the BAL fluids from Δ lasB (n = 8). C = Purified human SP-A. M1 – M8 = BAL of mice infected with P. aeruginosa . Western blot analyses were performed using a <t>polyclonal</t> antibody against SP-A. (D) Densitometry analysis of mSP-A degradation by PAO1, Δ lasB and PDO240lasB in mouse lungs. The amounts of remaining mSP-A in Δ lasB were set to the value of 100%. * p
    Msp A, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher arca capped mrna
    Elastase deficient Δ lasB mutant is attenuated in the degradation of SP-A during lung infection. (A-C) The amounts of intact <t>mSP-A</t> were not visibly changed at 6-hr (A) or 12- hr (B) post-infection. By 18 hr post-infection (C), intact mSP-A was reduced in the BAL fluid from PAO1- or PDO240lasB-infected SP-A +/+ mice (n = 6), suggesting that mSP-A was degraded in mouse lungs. In contrast, more abundant mSP-A was clearly visible in the BAL fluids from Δ lasB (n = 8). C = Purified human SP-A. M1 – M8 = BAL of mice infected with P. aeruginosa . Western blot analyses were performed using a <t>polyclonal</t> antibody against SP-A. (D) Densitometry analysis of mSP-A degradation by PAO1, Δ lasB and PDO240lasB in mouse lungs. The amounts of remaining mSP-A in Δ lasB were set to the value of 100%. * p
    Arca Capped Mrna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa episcope msp kit
    Elastase deficient Δ lasB mutant is attenuated in the degradation of SP-A during lung infection. (A-C) The amounts of intact <t>mSP-A</t> were not visibly changed at 6-hr (A) or 12- hr (B) post-infection. By 18 hr post-infection (C), intact mSP-A was reduced in the BAL fluid from PAO1- or PDO240lasB-infected SP-A +/+ mice (n = 6), suggesting that mSP-A was degraded in mouse lungs. In contrast, more abundant mSP-A was clearly visible in the BAL fluids from Δ lasB (n = 8). C = Purified human SP-A. M1 – M8 = BAL of mice infected with P. aeruginosa . Western blot analyses were performed using a <t>polyclonal</t> antibody against SP-A. (D) Densitometry analysis of mSP-A degradation by PAO1, Δ lasB and PDO240lasB in mouse lungs. The amounts of remaining mSP-A in Δ lasB were set to the value of 100%. * p
    Episcope Msp Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bluebird Bio arca bioharma
    Elastase deficient Δ lasB mutant is attenuated in the degradation of SP-A during lung infection. (A-C) The amounts of intact <t>mSP-A</t> were not visibly changed at 6-hr (A) or 12- hr (B) post-infection. By 18 hr post-infection (C), intact mSP-A was reduced in the BAL fluid from PAO1- or PDO240lasB-infected SP-A +/+ mice (n = 6), suggesting that mSP-A was degraded in mouse lungs. In contrast, more abundant mSP-A was clearly visible in the BAL fluids from Δ lasB (n = 8). C = Purified human SP-A. M1 – M8 = BAL of mice infected with P. aeruginosa . Western blot analyses were performed using a <t>polyclonal</t> antibody against SP-A. (D) Densitometry analysis of mSP-A degradation by PAO1, Δ lasB and PDO240lasB in mouse lungs. The amounts of remaining mSP-A in Δ lasB were set to the value of 100%. * p
    Arca Bioharma, supplied by Bluebird Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Isolated merozoites maintain surface coat integrity. A) E64-treated schizonts were filtered to release free merozoites and haemozoin crystals, and the filtrate was passed over magnetic columns. Merozoite purification was confirmed by Giemsa-stained smears of i) filtrate, ii) retained haemozoin and iii) purified merozoites. B) Merozites were stained with EtBr and enumerated by flow cytometry. C) Washed Merozoites retained surface proteins MSP-3, MSP-6 and AMA-1 by western blot (S: purified Schizonts; M: filtered merozoites; SM: EtBr stained merozoites). D) Merozoite surface proteins are maintained during merozoite isolation and wash steps as shown by surface localisation of MSP-3 and MSP1 19 by immunofluorescence microscopy. Antigens were stained with Alexa-594 and the nucleus with DAPI (panels in order Alexa549; DAPI; brightfield; Alexa549/DAPI; merge).

    Journal: PLoS ONE

    Article Title: Efficient Measurement of Opsonising Antibodies to Plasmodium falciparum Merozoites

    doi: 10.1371/journal.pone.0051692

    Figure Lengend Snippet: Isolated merozoites maintain surface coat integrity. A) E64-treated schizonts were filtered to release free merozoites and haemozoin crystals, and the filtrate was passed over magnetic columns. Merozoite purification was confirmed by Giemsa-stained smears of i) filtrate, ii) retained haemozoin and iii) purified merozoites. B) Merozites were stained with EtBr and enumerated by flow cytometry. C) Washed Merozoites retained surface proteins MSP-3, MSP-6 and AMA-1 by western blot (S: purified Schizonts; M: filtered merozoites; SM: EtBr stained merozoites). D) Merozoite surface proteins are maintained during merozoite isolation and wash steps as shown by surface localisation of MSP-3 and MSP1 19 by immunofluorescence microscopy. Antigens were stained with Alexa-594 and the nucleus with DAPI (panels in order Alexa549; DAPI; brightfield; Alexa549/DAPI; merge).

    Article Snippet: Reactvity of anti-MSP-3, MSP-6 and AMA-1 was compared by immunoblotting and detection by anti-rabbit or mouse IgG horseradish peroxidase (HRP) conjugate (Millipore), and visualised via enhanced chemiluminescence (ECL, Amersham Biosciences).

    Techniques: Isolation, Purification, Staining, Flow Cytometry, Cytometry, Western Blot, Immunofluorescence, Microscopy

    R1 and R3 peptides are specific for AMA1. (A) Two clones, R1 and R3, were identified from the pooled round-4 phages. R1 and R3 phages were incubated with wells coated with AMA1, MSP3, MSP2, RESA, and BSA and were found to be specific for AMA1. (B) Volumes

    Journal:

    Article Title: Binding Hot Spot for Invasion Inhibitory Molecules on Plasmodium falciparum Apical Membrane Antigen 1 †

    doi: 10.1128/IAI.73.10.6981-6989.2005

    Figure Lengend Snippet: R1 and R3 peptides are specific for AMA1. (A) Two clones, R1 and R3, were identified from the pooled round-4 phages. R1 and R3 phages were incubated with wells coated with AMA1, MSP3, MSP2, RESA, and BSA and were found to be specific for AMA1. (B) Volumes

    Article Snippet: Recombinant AMA1 and MSP3 (0.5 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis on 10% gels under nonreducing conditions and transferred to a polyvinylidene difluoride transfer membrane (PVDF-Plus; Millipore).

    Techniques: Clone Assay, Incubation

    Normalized DNA distributions of T-RFs in fractions from the 13 C-DCM labeled test (AE/AE-13D) and the unlabeled test (AE/AE-13N). a 337-bp T-RF digested with Hha I. b 398-bp T-RF digested with Msp I

    Journal: Water, Air, and Soil Pollution

    Article Title: Bacterial Degraders of Coexisting Dichloromethane, Benzene, and Toluene, Identified by Stable-Isotope Probing

    doi: 10.1007/s11270-017-3604-1

    Figure Lengend Snippet: Normalized DNA distributions of T-RFs in fractions from the 13 C-DCM labeled test (AE/AE-13D) and the unlabeled test (AE/AE-13N). a 337-bp T-RF digested with Hha I. b 398-bp T-RF digested with Msp I

    Article Snippet: The PCR products were purified with MinElute® PCR Purification Kit (QIAGEN), then independently digested with restriction enzymes: Hha I (Takara Bio) and Msp I (Takara Bio).

    Techniques: Labeling

    Normalized DNA distributions of T-RFs in fractions from the 13 C-benzene labeled test (AE/AE-13B) and unlabeled test (AE/AE-13N). a 352-bp T-RF digested with Hha I. b 154-bp T-RF digested with Msp I

    Journal: Water, Air, and Soil Pollution

    Article Title: Bacterial Degraders of Coexisting Dichloromethane, Benzene, and Toluene, Identified by Stable-Isotope Probing

    doi: 10.1007/s11270-017-3604-1

    Figure Lengend Snippet: Normalized DNA distributions of T-RFs in fractions from the 13 C-benzene labeled test (AE/AE-13B) and unlabeled test (AE/AE-13N). a 352-bp T-RF digested with Hha I. b 154-bp T-RF digested with Msp I

    Article Snippet: The PCR products were purified with MinElute® PCR Purification Kit (QIAGEN), then independently digested with restriction enzymes: Hha I (Takara Bio) and Msp I (Takara Bio).

    Techniques: Labeling

    Representative fragments in methylation sensitive amplification polymorphism (MSAP) profiles (A,B) and the relationship between U- values and ploidy level (C–E). (A) Hpa II and Msp I sensitivities to 5-CCGG methylation status (“+”: enzyme cuts; “-”: enzyme does not cut): three types of fragment generated. Type I: non-methylated, appearing in both the H and M lanes; Type II: fully methylated, present in the M but not the H lanes; Type III: hemi-methylated, present in the H but not the M lanes; (B) Examples of MSAP electrophoresis patterns in ABI3730xl; The relationship between U- values and ploidy level (C-E) . U- values associated with (C) total methylation, (D) full methylation, (E) hemi-methylation. A higher U -value implies a larger difference between different samples, but only U -values > 1.96 were statistically significant.

    Journal: Frontiers in Plant Science

    Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy

    doi: 10.3389/fpls.2015.00668

    Figure Lengend Snippet: Representative fragments in methylation sensitive amplification polymorphism (MSAP) profiles (A,B) and the relationship between U- values and ploidy level (C–E). (A) Hpa II and Msp I sensitivities to 5-CCGG methylation status (“+”: enzyme cuts; “-”: enzyme does not cut): three types of fragment generated. Type I: non-methylated, appearing in both the H and M lanes; Type II: fully methylated, present in the M but not the H lanes; Type III: hemi-methylated, present in the H but not the M lanes; (B) Examples of MSAP electrophoresis patterns in ABI3730xl; The relationship between U- values and ploidy level (C-E) . U- values associated with (C) total methylation, (D) full methylation, (E) hemi-methylation. A higher U -value implies a larger difference between different samples, but only U -values > 1.96 were statistically significant.

    Article Snippet: A 5 μL aliquot of the product was pre-amplified in the presence of 0.2 μM Eco RI and 0.2 μM Hpa II-Msp I non-selective primers (sequences given in Supplementary Table ) in a 25 μL reaction containing 2.5 μL 10x PCR buffer, 1.5 mM MgCl2 , 0.2 mM dNTP, and 2 U Taq polymerase (Takara, Japan).

    Techniques: Methylation, Amplification, Generated, Electrophoresis

    Elastase deficient Δ lasB mutant is attenuated in the degradation of SP-A during lung infection. (A-C) The amounts of intact mSP-A were not visibly changed at 6-hr (A) or 12- hr (B) post-infection. By 18 hr post-infection (C), intact mSP-A was reduced in the BAL fluid from PAO1- or PDO240lasB-infected SP-A +/+ mice (n = 6), suggesting that mSP-A was degraded in mouse lungs. In contrast, more abundant mSP-A was clearly visible in the BAL fluids from Δ lasB (n = 8). C = Purified human SP-A. M1 – M8 = BAL of mice infected with P. aeruginosa . Western blot analyses were performed using a polyclonal antibody against SP-A. (D) Densitometry analysis of mSP-A degradation by PAO1, Δ lasB and PDO240lasB in mouse lungs. The amounts of remaining mSP-A in Δ lasB were set to the value of 100%. * p

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa Elastase Provides an Escape from Phagocytosis by Degrading the Pulmonary Surfactant Protein-A

    doi: 10.1371/journal.pone.0027091

    Figure Lengend Snippet: Elastase deficient Δ lasB mutant is attenuated in the degradation of SP-A during lung infection. (A-C) The amounts of intact mSP-A were not visibly changed at 6-hr (A) or 12- hr (B) post-infection. By 18 hr post-infection (C), intact mSP-A was reduced in the BAL fluid from PAO1- or PDO240lasB-infected SP-A +/+ mice (n = 6), suggesting that mSP-A was degraded in mouse lungs. In contrast, more abundant mSP-A was clearly visible in the BAL fluids from Δ lasB (n = 8). C = Purified human SP-A. M1 – M8 = BAL of mice infected with P. aeruginosa . Western blot analyses were performed using a polyclonal antibody against SP-A. (D) Densitometry analysis of mSP-A degradation by PAO1, Δ lasB and PDO240lasB in mouse lungs. The amounts of remaining mSP-A in Δ lasB were set to the value of 100%. * p

    Article Snippet: The membranes were then incubated for 60 min at room temperature in blocking solution (PBS containing 3% BSA), followed by a 4-hr incubation with polyclonal antibody against hSP-A and mSP-A (Santa Cruz Biotecnology Inc, Santa Cruz, CA), a polyclonal antibody against chicken and mouse lysozymes , or with a polyclonal antibody against LasB , .

    Techniques: Mutagenesis, Infection, Mouse Assay, Purification, Western Blot