Journal: BMC Microbiology
Article Title: Genotypic comparison of Pantoea agglomerans plant and clinical strains
Figure Lengend Snippet: The fAFLP pattern generated with EcoRI-G and MseI-G primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
Article Snippet: Between 200-400 ng genomic DNA from each of the strains was used for each reaction in a mix containing 5 units EcoRI (Roche, Basel, Switzerland), 1 unit of MseI (Roche) and 1 unit of T4 DNA Ligase (Epicentre, Madison, U.S.A.), 5 mM 1,4-Dithio-DL-threitol (DTT) (Sigma-Aldrich, Buchs, Switzerland), 200 μM ATP (Fermentas, St. Leon-Rot, Germany), 50 μg/ml Bovine Serum Albumin (BSA), 0.25 μM of each EcoRI adaptor (EcoRI-F 5'-CTCGTAGACTGCGTACC-3', EcoRI-R 5'-AATTGGTACGCAGTCTAC-3') and 2.5 μM of each MseI adaptor (MseI-F 5'-GACGATGAGTCCTGAG-3', MseI-R 5'-TACTCAGGACTCAT-3') in a total volume of 11.1 μl of 1× One-Phor-All Buffer PLUS (GE Healthcare, Otelfingen, Switzerland).