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  • 95
    New England Biolabs mse i
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher tru1i msei restriction enzyme
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Tru1i Msei Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher msei
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Msei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    msei  (TaKaRa)
    89
    TaKaRa msei
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Msei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher msei adapters
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Msei Adapters, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Kaneka Corp msei adapter
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Msei Adapter, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore msei primer
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Msei Primer, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Genomed GmbH msei c primer
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Msei C Primer, supplied by Genomed GmbH, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Roche msei restriction enzyme
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Msei Restriction Enzyme, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Genomed GmbH msei ca primer
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Msei Ca Primer, supplied by Genomed GmbH, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher msei adaptor pairs
    Digitized AFLP patterns of Bacillus taxa generated using primer sets <t>EcoRI</t> plus <t>C/MseI</t> plus CA (A) and EcoRI plus C/MseI plus CC (B). Across the top of each image is the fragment size scale (in bases). The Bacillus species and strain designations for
    Msei Adaptor Pairs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Eurofins unlabeled msei n
    Digitized AFLP patterns of Bacillus taxa generated using primer sets <t>EcoRI</t> plus <t>C/MseI</t> plus CA (A) and EcoRI plus C/MseI plus CC (B). Across the top of each image is the fragment size scale (in bases). The Bacillus species and strain designations for
    Unlabeled Msei N, supplied by Eurofins, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher tru 1i
    Telomere length analysis in Selaginella moellendorffii . (A) Comparative terminal restriction fragment (TRF) analysis of S. moellendorffii (lane 1) and A. thaliana (lane 2) telomeres. Molecular weight markers are shown on the left. (B) Bal 31 digestion of S. moellendorffii telomeric DNA. Lane 1, <t>Tru</t> 1I digestion of genomic DNA without prior Bal treatment (0 min). Lanes 2–6, Tru 1I digestion of genomic DNA with Bal 31 tr31eatment for 15, 30, 45, 60, and 90 min, respectively. Asterisks indicate cross-hybridizing interstitial telomeric DNA bands, which are not sensitive to Bal 31 digestion for up to 90 min.
    Tru 1i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher msei cat
    Telomere length analysis in Selaginella moellendorffii . (A) Comparative terminal restriction fragment (TRF) analysis of S. moellendorffii (lane 1) and A. thaliana (lane 2) telomeres. Molecular weight markers are shown on the left. (B) Bal 31 digestion of S. moellendorffii telomeric DNA. Lane 1, <t>Tru</t> 1I digestion of genomic DNA without prior Bal treatment (0 min). Lanes 2–6, Tru 1I digestion of genomic DNA with Bal 31 tr31eatment for 15, 30, 45, 60, and 90 min, respectively. Asterisks indicate cross-hybridizing interstitial telomeric DNA bands, which are not sensitive to Bal 31 digestion for up to 90 min.
    Msei Cat, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    msei  (Roche)
    88
    Roche msei
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Msei, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher msei restriction enzymes
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Msei Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher restriction endonuclease msei
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Restriction Endonuclease Msei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    New England Biolabs msei buffer
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Msei Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs msei enzyme
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Msei Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    New England Biolabs endonuclease msei
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Endonuclease Msei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 83/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher msei primer
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Msei Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    New England Biolabs msei ttaa
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Msei Ttaa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher msei ttaa
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Msei Ttaa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Silicon Biosystems ttaa msei restriction site motifs
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Ttaa Msei Restriction Site Motifs, supplied by Silicon Biosystems, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Integrated DNA Technologies msei 3nt primers
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
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    New England Biolabs msei t↓taa
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
    Msei T↓Taa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrated DNA Technologies msei 5 gatgagtcctgagtaa
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
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    New England Biolabs 4 cutter msei
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
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    Thermo Fisher restriction enzyme msei
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
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    New England Biolabs psti msei
    The fAFLP pattern generated with <t>EcoRI-G</t> and <t>MseI-G</t> primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.
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    Image Search Results


    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the DNA substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by Mse I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.

    Journal: PLoS ONE

    Article Title: A Photolyase-Like Protein from Agrobacterium tumefaciens with an Iron-Sulfur Cluster

    doi: 10.1371/journal.pone.0026775

    Figure Lengend Snippet: Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the DNA substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by Mse I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.

    Article Snippet: Subsequently, proteins were inactivated by heating to 95°C for 10 min and the DNA probe was digested with Mse I (NEB).

    Techniques: In Vivo, Mutagenesis, Irradiation, In Vitro, Incubation

    Digitized AFLP patterns of Bacillus taxa generated using primer sets EcoRI plus C/MseI plus CA (A) and EcoRI plus C/MseI plus CC (B). Across the top of each image is the fragment size scale (in bases). The Bacillus species and strain designations for

    Journal: Applied and Environmental Microbiology

    Article Title: Detection of Molecular Diversity in Bacillus atrophaeus by Amplified Fragment Length Polymorphism Analysis

    doi: 10.1128/AEM.70.5.2786-2790.2004

    Figure Lengend Snippet: Digitized AFLP patterns of Bacillus taxa generated using primer sets EcoRI plus C/MseI plus CA (A) and EcoRI plus C/MseI plus CC (B). Across the top of each image is the fragment size scale (in bases). The Bacillus species and strain designations for

    Article Snippet: EcoRI and MseI adaptor pairs (Applied Biosystems) were ligated to restriction fragments according to the manufacturer's protocol.

    Techniques: Generated

    Telomere length analysis in Selaginella moellendorffii . (A) Comparative terminal restriction fragment (TRF) analysis of S. moellendorffii (lane 1) and A. thaliana (lane 2) telomeres. Molecular weight markers are shown on the left. (B) Bal 31 digestion of S. moellendorffii telomeric DNA. Lane 1, Tru 1I digestion of genomic DNA without prior Bal treatment (0 min). Lanes 2–6, Tru 1I digestion of genomic DNA with Bal 31 tr31eatment for 15, 30, 45, 60, and 90 min, respectively. Asterisks indicate cross-hybridizing interstitial telomeric DNA bands, which are not sensitive to Bal 31 digestion for up to 90 min.

    Journal: Frontiers in Plant Science

    Article Title: Selaginella moellendorffii telomeres: conserved and unique features in an ancient land plant lineage

    doi: 10.3389/fpls.2012.00161

    Figure Lengend Snippet: Telomere length analysis in Selaginella moellendorffii . (A) Comparative terminal restriction fragment (TRF) analysis of S. moellendorffii (lane 1) and A. thaliana (lane 2) telomeres. Molecular weight markers are shown on the left. (B) Bal 31 digestion of S. moellendorffii telomeric DNA. Lane 1, Tru 1I digestion of genomic DNA without prior Bal treatment (0 min). Lanes 2–6, Tru 1I digestion of genomic DNA with Bal 31 tr31eatment for 15, 30, 45, 60, and 90 min, respectively. Asterisks indicate cross-hybridizing interstitial telomeric DNA bands, which are not sensitive to Bal 31 digestion for up to 90 min.

    Article Snippet: To detect telomeric DNA repeats, genomic DNA was digested with Tru 1I (Fermentas; recognition sequence TTAA) and subjected to Southern blotting with 32 P-labeled (TTTAGGG)4 as a probe ( ).

    Techniques: Molecular Weight

    The fAFLP pattern generated with EcoRI-G and MseI-G primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.

    Journal: BMC Microbiology

    Article Title: Genotypic comparison of Pantoea agglomerans plant and clinical strains

    doi: 10.1186/1471-2180-9-204

    Figure Lengend Snippet: The fAFLP pattern generated with EcoRI-G and MseI-G primers from different biocontrol, environmental and clinical P. agglomerans isolates . ( A ) C9-1, ( B ) CIP 82.100, ( C ) P10c, ( D ) Eh325, ( E ) EM21cb, ( F ) EM22cb, ( G ) CIP A181. Biocontrol strain P. agglomerans C9-1 showed a completely divergent fAFLP pattern with respect to the other isolates of the species. The 490-bp band which was prevalent in biocontrol and environmental isolates, but was absent from clinical isolates and from strain C9-1 is indicated by the arrow.

    Article Snippet: Between 200-400 ng genomic DNA from each of the strains was used for each reaction in a mix containing 5 units EcoRI (Roche, Basel, Switzerland), 1 unit of MseI (Roche) and 1 unit of T4 DNA Ligase (Epicentre, Madison, U.S.A.), 5 mM 1,4-Dithio-DL-threitol (DTT) (Sigma-Aldrich, Buchs, Switzerland), 200 μM ATP (Fermentas, St. Leon-Rot, Germany), 50 μg/ml Bovine Serum Albumin (BSA), 0.25 μM of each EcoRI adaptor (EcoRI-F 5'-CTCGTAGACTGCGTACC-3', EcoRI-R 5'-AATTGGTACGCAGTCTAC-3') and 2.5 μM of each MseI adaptor (MseI-F 5'-GACGATGAGTCCTGAG-3', MseI-R 5'-TACTCAGGACTCAT-3') in a total volume of 11.1 μl of 1× One-Phor-All Buffer PLUS (GE Healthcare, Otelfingen, Switzerland).

    Techniques: Generated