mse i New England Biolabs Search Results


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  • 99
    New England Biolabs mse i mse
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse I Mse, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mse i  (Roche)
    92
    Roche mse i
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse I, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    MWG-Biotech mse i
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse I, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher mse i adaptors
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse I Adaptors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Integrated DNA Technologies mse
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher mse i
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs eco ri mse
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Eco Ri Mse, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher mse i oligonucleotide adapters
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse I Oligonucleotide Adapters, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TaKaRa mse i
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    New England Biolabs mse i restriction enzyme
    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the <t>DNA</t> substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by <t>Mse</t> I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.
    Mse I Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    New England Biolabs restriction nuclease mse i
    Nano-ESI mass spectra of unmethylated <t>DNA.</t> ( a ) Nano-ESI mass spectrum of the mixture of for40 and rev40. ( b ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol II: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with MilliQ water, and desalt with a P-6 spin column equilibrated with MilliQ water. The desalted sample was mixed with 2 M TEA in acetonitrile at a ratio of 1:1. ( c ) Nano-ESI mass spectrum of the <t>Mse</t> I digest of 147 bp DNA prepared by protocol II. ( d ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol III: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with 50 mM ammonium acetate, and desalt with a P-6 spin column equilibrated with 20 mM ammonium acetate. The desalted sample was mixed with methanol at a ratio of 1:1. Insets in ( a ), ( b ), and ( d ) indicate expanded mass spectra, showing the most intense peaks. Red and blue reversed triangles correspond to for40 and rev40, respectively. Black closed circles in (d) indicate peaks of double-stranded 40 bp DNA
    Restriction Nuclease Mse I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    New England Biolabs mse i restriction enzymes
    Nano-ESI mass spectra of unmethylated <t>DNA.</t> ( a ) Nano-ESI mass spectrum of the mixture of for40 and rev40. ( b ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol II: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with MilliQ water, and desalt with a P-6 spin column equilibrated with MilliQ water. The desalted sample was mixed with 2 M TEA in acetonitrile at a ratio of 1:1. ( c ) Nano-ESI mass spectrum of the <t>Mse</t> I digest of 147 bp DNA prepared by protocol II. ( d ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol III: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with 50 mM ammonium acetate, and desalt with a P-6 spin column equilibrated with 20 mM ammonium acetate. The desalted sample was mixed with methanol at a ratio of 1:1. Insets in ( a ), ( b ), and ( d ) indicate expanded mass spectra, showing the most intense peaks. Red and blue reversed triangles correspond to for40 and rev40, respectively. Black closed circles in (d) indicate peaks of double-stranded 40 bp DNA
    Mse I Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    New England Biolabs eco r i mse i
    Nano-ESI mass spectra of unmethylated <t>DNA.</t> ( a ) Nano-ESI mass spectrum of the mixture of for40 and rev40. ( b ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol II: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with MilliQ water, and desalt with a P-6 spin column equilibrated with MilliQ water. The desalted sample was mixed with 2 M TEA in acetonitrile at a ratio of 1:1. ( c ) Nano-ESI mass spectrum of the <t>Mse</t> I digest of 147 bp DNA prepared by protocol II. ( d ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol III: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with 50 mM ammonium acetate, and desalt with a P-6 spin column equilibrated with 20 mM ammonium acetate. The desalted sample was mixed with methanol at a ratio of 1:1. Insets in ( a ), ( b ), and ( d ) indicate expanded mass spectra, showing the most intense peaks. Red and blue reversed triangles correspond to for40 and rev40, respectively. Black closed circles in (d) indicate peaks of double-stranded 40 bp DNA
    Eco R I Mse I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    New England Biolabs restriction endonucleases mse i
    Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with <t>Mse</t> I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)
    Restriction Endonucleases Mse I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    New England Biolabs mse i bgl ii
    Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with <t>Mse</t> I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)
    Mse I Bgl Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the DNA substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by Mse I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.

    Journal: PLoS ONE

    Article Title: A Photolyase-Like Protein from Agrobacterium tumefaciens with an Iron-Sulfur Cluster

    doi: 10.1371/journal.pone.0026775

    Figure Lengend Snippet: Photorepair of UV-lesions. (A) In vivo photoreactivation test. A. tumefaciens cells of the wild type (WT), the phrA − and the phrB − mutant were spread on LB agar plates, irradiated with UV-B light for 5 min and immediately transferred to darkness (−) or subjected to photoreactivating white light (+). Mean values of CFU ± SE; n = 8 to 14. (B and C) In vitro repair of pyrimidine dimers by PhrA and PhrB. Thymine dimers were created by UV-C illumination of the DNA substrate (first row, +/− UV-C), mixed with enzyme (second row) and incubated for the indicated times in photoreactivating blue light (405 nm, 150 µ mol m −2 s −1 ) or in darkness (*) (third row). Photorepaired DNA can be digested by Mse I (fourth row, +/− Mse I). Shown are representative gels from three independent repair experiments using PhrA or PhrB.

    Article Snippet: Subsequently, proteins were inactivated by heating to 95°C for 10 min and the DNA probe was digested with Mse I (NEB).

    Techniques: In Vivo, Mutagenesis, Irradiation, In Vitro, Incubation

    RFLP patterns of T . pallidum subsp. pertenue . (A) The tpr E , G , and J genes were amplified in a nested PCR to produce a mixed amplicon approximately 1830–1848 bp in length. Following PCR, amplicons were digested with the enzyme Mse I and analyzed on an Agilent Bioanalyzer. Two RFLP patterns ( q and r ) were observed among the clinical specimens and laboratory strains analyzed, including those for strains only partially typed (data not shown). The sizes of each fragment were confirmed through sequencing and are indicated in red. The Nichols TPA strain was used as a control for RFLP. The violet and green bands visible in each lane depict the Bioanalyzer kit’s internal control upper and lower bands, respectively. (B) Each of the tpr E , G , and J genes was amplified directly from the genome and sequenced. Regions of the products between the IP6 and IP7 primer annealing sequences were analyzed for Mse I restriction sites for both patterns. Approximate positions of each site (indicated as “M”) are shown. Sizes between each restriction site are listed in red.

    Journal: PLoS ONE

    Article Title: Molecular strain typing of the yaws pathogen, Treponema pallidum subspecies pertenue

    doi: 10.1371/journal.pone.0203632

    Figure Lengend Snippet: RFLP patterns of T . pallidum subsp. pertenue . (A) The tpr E , G , and J genes were amplified in a nested PCR to produce a mixed amplicon approximately 1830–1848 bp in length. Following PCR, amplicons were digested with the enzyme Mse I and analyzed on an Agilent Bioanalyzer. Two RFLP patterns ( q and r ) were observed among the clinical specimens and laboratory strains analyzed, including those for strains only partially typed (data not shown). The sizes of each fragment were confirmed through sequencing and are indicated in red. The Nichols TPA strain was used as a control for RFLP. The violet and green bands visible in each lane depict the Bioanalyzer kit’s internal control upper and lower bands, respectively. (B) Each of the tpr E , G , and J genes was amplified directly from the genome and sequenced. Regions of the products between the IP6 and IP7 primer annealing sequences were analyzed for Mse I restriction sites for both patterns. Approximate positions of each site (indicated as “M”) are shown. Sizes between each restriction site are listed in red.

    Article Snippet: Reaction conditions were as follows: 94°C for 5 min, 35 cycles of 94°C for 30 sec, 60°C for 1 min, and 68°C for 2 min 30 sec, followed by a final extension at 68°C for 15 min. Two μL of the outer PCR product was used for the nested reaction using the following conditions using primers IP6 and IP7: 94°C for 5 min, 40 cycles of 94°C for 30 sec, 59°C for 1 min, and 68°C for 2 min, followed by a final extension at 68°C for 15 min. RFLP analysis was performed on the nested tpr PCR product by digesting 6 μL of the DNA in a 10 μL volume with 1x reaction buffer, 1x bovine serum albumin (BSA), and 2 units of the Mse I enzyme (New England Biolabs, Ipswich, MA, USA).

    Techniques: Amplification, Nested PCR, Polymerase Chain Reaction, Sequencing

    Nano-ESI mass spectra of unmethylated DNA. ( a ) Nano-ESI mass spectrum of the mixture of for40 and rev40. ( b ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol II: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with MilliQ water, and desalt with a P-6 spin column equilibrated with MilliQ water. The desalted sample was mixed with 2 M TEA in acetonitrile at a ratio of 1:1. ( c ) Nano-ESI mass spectrum of the Mse I digest of 147 bp DNA prepared by protocol II. ( d ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol III: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with 50 mM ammonium acetate, and desalt with a P-6 spin column equilibrated with 20 mM ammonium acetate. The desalted sample was mixed with methanol at a ratio of 1:1. Insets in ( a ), ( b ), and ( d ) indicate expanded mass spectra, showing the most intense peaks. Red and blue reversed triangles correspond to for40 and rev40, respectively. Black closed circles in (d) indicate peaks of double-stranded 40 bp DNA

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Rapid and Definitive Analysis of In Vitro DNA Methylation by Nano-electrospray Ionization Mass Spectrometry

    doi: 10.1007/s13361-019-02304-5

    Figure Lengend Snippet: Nano-ESI mass spectra of unmethylated DNA. ( a ) Nano-ESI mass spectrum of the mixture of for40 and rev40. ( b ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol II: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with MilliQ water, and desalt with a P-6 spin column equilibrated with MilliQ water. The desalted sample was mixed with 2 M TEA in acetonitrile at a ratio of 1:1. ( c ) Nano-ESI mass spectrum of the Mse I digest of 147 bp DNA prepared by protocol II. ( d ) Nano-ESI mass spectrum of 40 bp DNA prepared by protocol III: ethanol precipitation in the presence of 7 M ammonium acetate, solubilization with 50 mM ammonium acetate, and desalt with a P-6 spin column equilibrated with 20 mM ammonium acetate. The desalted sample was mixed with methanol at a ratio of 1:1. Insets in ( a ), ( b ), and ( d ) indicate expanded mass spectra, showing the most intense peaks. Red and blue reversed triangles correspond to for40 and rev40, respectively. Black closed circles in (d) indicate peaks of double-stranded 40 bp DNA

    Article Snippet: For 40 and 147 bp DNA, digestion with the restriction nuclease Mse I (New England Biolabs, Ipswich, MA), which recognizes the TTAA sequence was performed.

    Techniques: Ethanol Precipitation

    Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with Mse I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)

    Journal: Emerging Infectious Diseases

    Article Title: Novel Cryptosporidium Genotypes in Sporadic Cryptosporidiosis Cases: First Report of Human Infections with a Cervine Genotype

    doi: 10.3201/eid0803.010194

    Figure Lengend Snippet: Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with Mse I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)

    Article Snippet: RFLP Analyses of PCR Products PCR products were purified by using QIAquick spin columns (Qiagen, Mississauga, ON) according to the manufacturer’s instructions before digestion with the restriction endonucleases Mse I (New England BioLabs, Mississauga, ON) for the ITS1 locus and Rsa I (New England BioLabs) for the COWP gene.

    Techniques: Polymerase Chain Reaction, Molecular Weight