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  • 99
    New England Biolabs mse i mse
    Mse I Mse, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mse i mse/product/New England Biolabs
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    mse i mse - by Bioz Stars, 2020-08
    99/100 stars
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    93
    Thermo Fisher gene exp eno3 hs01093275 m1
    STK11 mutation-dependent anticancer efficacy of <t>ENO3</t> gene knockdown Average variation in cell proliferation (A) and cell viability (B) of STK11 wild-type cell lines (H322M, HCC827, and H1975) and mutant cell lines (A549, H23, and H1993) by the ENO3 gene knockdown using siRNAs. Comparative changes in cell proliferation (C) and cell viability (D) in STK11 -mutant parental cells and STK11 recovered cells by the ENO3 gene knockdown. Cell proliferation was measured based on the direct cell count in the well. Cell viability was measured using CellTiter Blue assay (see the Materials and Methods section for detail). All y-axes represent percentage of cell proliferation or cell viability divided by an average of negative control. Student’s one-tailed t -test was used to test statistical significance. Error bars indicate SD.
    Gene Exp Eno3 Hs01093275 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp eno3 hs01093275 m1/product/Thermo Fisher
    Average 93 stars, based on 3 article reviews
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    92
    Millipore eno3
    Validation of protein expression changes with different technologies. (a) Heatmap showing the log fold change values (in S5 Data ) of proteins and mRNA DE in Th17 and iTreg cells in comparison with Th0 cells and Th17 compared with iTreg cells. (b) Flow cytometry analysis demonstrating the expression of surface molecules CD69 and CD101 in murine Th0, iTreg, and Th17 cells. One replicate is shown. (c) Immunoblot analysis of DE proteins in iTreg and Th17 cells compared to Th0 cells. Representative blots from 2–3 independent experiments are shown. DE, differentially expressed; <t>ENO3,</t> enolase 3; FOXO1, forkhead box O1; iTreg, induced regulatory T; NFATC2, nuclear factor of activated T cells 2; PSMB5, proteasome subunit beta 5; SMYD3, SET and MYND domain containing 3; Th0, T cell receptor–activated helper T; Th17, T helper 17; Thp, naïve CD4+ T; VIM, vimentin.
    Eno3, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eno3/product/Millipore
    Average 92 stars, based on 8 article reviews
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    eno3 - by Bioz Stars, 2020-08
    92/100 stars
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    93
    Thermo Fisher eno3
    STK11 mutation-dependent anticancer efficacy of <t>ENO3</t> gene knockdown Average variation in cell proliferation (A) and cell viability (B) of STK11 wild-type cell lines (H322M, HCC827, and H1975) and mutant cell lines (A549, H23, and H1993) by the ENO3 gene knockdown using siRNAs. Comparative changes in cell proliferation (C) and cell viability (D) in STK11 -mutant parental cells and STK11 recovered cells by the ENO3 gene knockdown. Cell proliferation was measured based on the direct cell count in the well. Cell viability was measured using CellTiter Blue assay (see the Materials and Methods section for detail). All y-axes represent percentage of cell proliferation or cell viability divided by an average of negative control. Student’s one-tailed t -test was used to test statistical significance. Error bars indicate SD.
    Eno3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology eno3
    Increased expression levels of ENO1 in hypertensive APAH patients and experimental PH animal lungs. a PASMC isolated from patients with IPAH or APAH and control donors were lysed and subjected to western blotting to measure the level of enolases. b Normalized quantification of proteins demonstrates the expression level of ENO1, ENO2, and <t>ENO3</t> in PASMC isolated from patients with IPAH or APAH and control donors ( n = 6 per group, * P = 0.0223). c mRNA levels of ENO1, ENO2, and ENO3 in PASMC from patients with PAH and the control donors. d Immunohistochemistry of ENO1 in lung tissue sections of patients with PAH and the control donors. Brown color indicates the staining of ENO1. Black arrows indicate the elevated of ENO1 staining in the PASMC layer of the vessels (scale bars, 100 μm). e Whole lung tissues were isolated from hypoxia-induced PH mice and western blotting was used to measure the level of enolases. f Normalized quantification of proteins demonstrates the expression level of enolases in the whole lung tissue of hypoxia-induced PH mice ( n = 4 per group, * P = 0.0263). g mRNA levels of enolase in the whole lung tissue of hypoxia-induced PH mice ( n = 3–6, P
    Eno3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eno3/product/Santa Cruz Biotechnology
    Average 92 stars, based on 4 article reviews
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    eno3 - by Bioz Stars, 2020-08
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    87
    Thermo Fisher gene exp eno3 mm00468267 m1
    Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , <t>Eno3</t> , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p
    Gene Exp Eno3 Mm00468267 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eno3  (Abcam)
    91
    Abcam eno3
    Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , <t>Eno3</t> , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p
    Eno3, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eno3 - by Bioz Stars, 2020-08
    91/100 stars
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    89
    OriGene eno3
    Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , <t>Eno3</t> , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p
    Eno3, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Proteintech proteintech eno3
    Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , <t>Eno3</t> , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p
    Proteintech Eno3, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore anti eno3 ab1 antibody
    Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , <t>Eno3</t> , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p
    Anti Eno3 Ab1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson eno3
    Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , <t>Eno3</t> , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p
    Eno3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega mse i
    Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , <t>Eno3</t> , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p
    Mse I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mse i  (Roche)
    92
    Roche mse i
    Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , <t>Eno3</t> , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p
    Mse I, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher mse i
    Amplified fragment length polymorphic complementary DNA <t>(AFLP-cDNA)</t> display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and <t>Mse</t> I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.
    Mse I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Sanyo mse microcentaur
    Amplified fragment length polymorphic complementary DNA <t>(AFLP-cDNA)</t> display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and <t>Mse</t> I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.
    Mse Microcentaur, supplied by Sanyo, used in various techniques. Bioz Stars score: 85/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Difco mse agar
    Amplified fragment length polymorphic complementary DNA <t>(AFLP-cDNA)</t> display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and <t>Mse</t> I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.
    Mse Agar, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    MSE Ltd mse homogenizer
    Amplified fragment length polymorphic complementary DNA <t>(AFLP-cDNA)</t> display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and <t>Mse</t> I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.
    Mse Homogenizer, supplied by MSE Ltd, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Molecular Devices LLC spectramax mse
    Amplified fragment length polymorphic complementary DNA <t>(AFLP-cDNA)</t> display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and <t>Mse</t> I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.
    Spectramax Mse, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 86/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Sanyo mse sonicator
    Amplified fragment length polymorphic complementary DNA <t>(AFLP-cDNA)</t> display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and <t>Mse</t> I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.
    Mse Sonicator, supplied by Sanyo, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim mse i
    Amplified fragment length polymorphic complementary DNA <t>(AFLP-cDNA)</t> display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and <t>Mse</t> I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.
    Mse I, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Amplified fragment length polymorphic complementary DNA <t>(AFLP-cDNA)</t> display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and <t>Mse</t> I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.
    Mse I, supplied by KeyGene, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MWG-Biotech mse i
    Amplified fragment length polymorphic complementary DNA <t>(AFLP-cDNA)</t> display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and <t>Mse</t> I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.
    Mse I, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    STK11 mutation-dependent anticancer efficacy of ENO3 gene knockdown Average variation in cell proliferation (A) and cell viability (B) of STK11 wild-type cell lines (H322M, HCC827, and H1975) and mutant cell lines (A549, H23, and H1993) by the ENO3 gene knockdown using siRNAs. Comparative changes in cell proliferation (C) and cell viability (D) in STK11 -mutant parental cells and STK11 recovered cells by the ENO3 gene knockdown. Cell proliferation was measured based on the direct cell count in the well. Cell viability was measured using CellTiter Blue assay (see the Materials and Methods section for detail). All y-axes represent percentage of cell proliferation or cell viability divided by an average of negative control. Student’s one-tailed t -test was used to test statistical significance. Error bars indicate SD.

    Journal: Molecules and Cells

    Article Title: Overexpression and Selective Anticancer Efficacy of ENO3 in STK11 Mutant Lung Cancers

    doi: 10.14348/molcells.2019.0099

    Figure Lengend Snippet: STK11 mutation-dependent anticancer efficacy of ENO3 gene knockdown Average variation in cell proliferation (A) and cell viability (B) of STK11 wild-type cell lines (H322M, HCC827, and H1975) and mutant cell lines (A549, H23, and H1993) by the ENO3 gene knockdown using siRNAs. Comparative changes in cell proliferation (C) and cell viability (D) in STK11 -mutant parental cells and STK11 recovered cells by the ENO3 gene knockdown. Cell proliferation was measured based on the direct cell count in the well. Cell viability was measured using CellTiter Blue assay (see the Materials and Methods section for detail). All y-axes represent percentage of cell proliferation or cell viability divided by an average of negative control. Student’s one-tailed t -test was used to test statistical significance. Error bars indicate SD.

    Article Snippet: The gene expression of ENO1 , ENO2 , and ENO3 was quantified via real-time PCR using specific primer sets targeting ENO1 (Hs00157360_m1, TaqMan), ENO2 (Hs00361415_m1, TagMan), and ENO3 (Hs01093275_m1, TaqMan), respectively, and the reference gene GAPDH (Hs02786624_g1, TagMan) ( ; ).

    Techniques: Mutagenesis, Cell Counting, CtB Assay, Negative Control, One-tailed Test

    Expression of ENO gene family in DNA microarray data and qPCR experiment using LUAD cell lines (A) Comparison of ENO family gene expression (ENO1: 201231_s_at, ENO2: 201313_at, and ENO3: 204483_at) between nine STK11 -mutant and 38 STK11 wild-type cell lines in the CCLE dataset. Wilcoxon rank-sum test for ENO3 and Student’s t -test for others were used to test statistical significance. (B) Experimental validation (qPCR) of ENO family gene expression in STK11 wide-type cell lines (H322M, HCC827, and H1975), STK11 -mutant parental cell lines (A549, H23, and H1993) and STK11 -restored cell lines. The expression level of GAPDH was observed to be varied within SD

    Journal: Molecules and Cells

    Article Title: Overexpression and Selective Anticancer Efficacy of ENO3 in STK11 Mutant Lung Cancers

    doi: 10.14348/molcells.2019.0099

    Figure Lengend Snippet: Expression of ENO gene family in DNA microarray data and qPCR experiment using LUAD cell lines (A) Comparison of ENO family gene expression (ENO1: 201231_s_at, ENO2: 201313_at, and ENO3: 204483_at) between nine STK11 -mutant and 38 STK11 wild-type cell lines in the CCLE dataset. Wilcoxon rank-sum test for ENO3 and Student’s t -test for others were used to test statistical significance. (B) Experimental validation (qPCR) of ENO family gene expression in STK11 wide-type cell lines (H322M, HCC827, and H1975), STK11 -mutant parental cell lines (A549, H23, and H1993) and STK11 -restored cell lines. The expression level of GAPDH was observed to be varied within SD

    Article Snippet: The gene expression of ENO1 , ENO2 , and ENO3 was quantified via real-time PCR using specific primer sets targeting ENO1 (Hs00157360_m1, TaqMan), ENO2 (Hs00361415_m1, TagMan), and ENO3 (Hs01093275_m1, TaqMan), respectively, and the reference gene GAPDH (Hs02786624_g1, TagMan) ( ; ).

    Techniques: Expressing, Microarray, Real-time Polymerase Chain Reaction, Mutagenesis

    Validation of protein expression changes with different technologies. (a) Heatmap showing the log fold change values (in S5 Data ) of proteins and mRNA DE in Th17 and iTreg cells in comparison with Th0 cells and Th17 compared with iTreg cells. (b) Flow cytometry analysis demonstrating the expression of surface molecules CD69 and CD101 in murine Th0, iTreg, and Th17 cells. One replicate is shown. (c) Immunoblot analysis of DE proteins in iTreg and Th17 cells compared to Th0 cells. Representative blots from 2–3 independent experiments are shown. DE, differentially expressed; ENO3, enolase 3; FOXO1, forkhead box O1; iTreg, induced regulatory T; NFATC2, nuclear factor of activated T cells 2; PSMB5, proteasome subunit beta 5; SMYD3, SET and MYND domain containing 3; Th0, T cell receptor–activated helper T; Th17, T helper 17; Thp, naïve CD4+ T; VIM, vimentin.

    Journal: PLoS Biology

    Article Title: Quantitative proteomic characterization and comparison of T helper 17 and induced regulatory T cells

    doi: 10.1371/journal.pbio.2004194

    Figure Lengend Snippet: Validation of protein expression changes with different technologies. (a) Heatmap showing the log fold change values (in S5 Data ) of proteins and mRNA DE in Th17 and iTreg cells in comparison with Th0 cells and Th17 compared with iTreg cells. (b) Flow cytometry analysis demonstrating the expression of surface molecules CD69 and CD101 in murine Th0, iTreg, and Th17 cells. One replicate is shown. (c) Immunoblot analysis of DE proteins in iTreg and Th17 cells compared to Th0 cells. Representative blots from 2–3 independent experiments are shown. DE, differentially expressed; ENO3, enolase 3; FOXO1, forkhead box O1; iTreg, induced regulatory T; NFATC2, nuclear factor of activated T cells 2; PSMB5, proteasome subunit beta 5; SMYD3, SET and MYND domain containing 3; Th0, T cell receptor–activated helper T; Th17, T helper 17; Thp, naïve CD4+ T; VIM, vimentin.

    Article Snippet: The antibodies used in this study are NFATC2, PSMB5, VIM (all from Cell Signaling), ENO3 (Sigma), FOXO1 (Immunoway), SMYD3 (Abcam), FOXP3 (eBioscience), and β-actin (CalBioChem).

    Techniques: Expressing, Flow Cytometry, Cytometry

    Correlation of protein and RNA expression changes during Th17 and iTreg cell differentiation. Venn diagram showing the comparison of DE proteins with corresponding transcripts and DE transcripts with encoded detected proteins in comparison of Th17 and Th0 cells (a) or iTreg versus Thp cells (b). Scatterplot of proteins that were observed in proteomic and transcriptomic comparison of Th17 versus Th0 cell (a) or iTreg versus Thp cells (b). The lists of detected proteins and transcripts in Th17 versus Th0 cells and in iTreg versus Thp cells are in S4 Data . Ahr, aryl hydrocarbon receptor; Cnot2, CCR4-NOT transcription complex subunit 2; Coa6, cytochrome c oxidase assembly factor 6; DE, differentially expressed; Eno3, enolase 3; Foxo1, forkhead box O1; Foxp3, forkhead box P3; Gimap5, GTPase IMAP family member 5; Il17f, interleukin 17F; Isg15, interferon-stimulated gene 15; iTreg, induced regulatory T; Psmb5, proteasome subunit beta 5; Rorc, retinoic acid receptor–related orphan receptor C; Th0, T cell receptor–activated helper T; Th17, T helper 17; Thp, naïve CD4+ T.

    Journal: PLoS Biology

    Article Title: Quantitative proteomic characterization and comparison of T helper 17 and induced regulatory T cells

    doi: 10.1371/journal.pbio.2004194

    Figure Lengend Snippet: Correlation of protein and RNA expression changes during Th17 and iTreg cell differentiation. Venn diagram showing the comparison of DE proteins with corresponding transcripts and DE transcripts with encoded detected proteins in comparison of Th17 and Th0 cells (a) or iTreg versus Thp cells (b). Scatterplot of proteins that were observed in proteomic and transcriptomic comparison of Th17 versus Th0 cell (a) or iTreg versus Thp cells (b). The lists of detected proteins and transcripts in Th17 versus Th0 cells and in iTreg versus Thp cells are in S4 Data . Ahr, aryl hydrocarbon receptor; Cnot2, CCR4-NOT transcription complex subunit 2; Coa6, cytochrome c oxidase assembly factor 6; DE, differentially expressed; Eno3, enolase 3; Foxo1, forkhead box O1; Foxp3, forkhead box P3; Gimap5, GTPase IMAP family member 5; Il17f, interleukin 17F; Isg15, interferon-stimulated gene 15; iTreg, induced regulatory T; Psmb5, proteasome subunit beta 5; Rorc, retinoic acid receptor–related orphan receptor C; Th0, T cell receptor–activated helper T; Th17, T helper 17; Thp, naïve CD4+ T.

    Article Snippet: The antibodies used in this study are NFATC2, PSMB5, VIM (all from Cell Signaling), ENO3 (Sigma), FOXO1 (Immunoway), SMYD3 (Abcam), FOXP3 (eBioscience), and β-actin (CalBioChem).

    Techniques: RNA Expression, Cell Differentiation

    STK11 mutation-dependent anticancer efficacy of ENO3 gene knockdown Average variation in cell proliferation (A) and cell viability (B) of STK11 wild-type cell lines (H322M, HCC827, and H1975) and mutant cell lines (A549, H23, and H1993) by the ENO3 gene knockdown using siRNAs. Comparative changes in cell proliferation (C) and cell viability (D) in STK11 -mutant parental cells and STK11 recovered cells by the ENO3 gene knockdown. Cell proliferation was measured based on the direct cell count in the well. Cell viability was measured using CellTiter Blue assay (see the Materials and Methods section for detail). All y-axes represent percentage of cell proliferation or cell viability divided by an average of negative control. Student’s one-tailed t -test was used to test statistical significance. Error bars indicate SD.

    Journal: Molecules and Cells

    Article Title: Overexpression and Selective Anticancer Efficacy of ENO3 in STK11 Mutant Lung Cancers

    doi: 10.14348/molcells.2019.0099

    Figure Lengend Snippet: STK11 mutation-dependent anticancer efficacy of ENO3 gene knockdown Average variation in cell proliferation (A) and cell viability (B) of STK11 wild-type cell lines (H322M, HCC827, and H1975) and mutant cell lines (A549, H23, and H1993) by the ENO3 gene knockdown using siRNAs. Comparative changes in cell proliferation (C) and cell viability (D) in STK11 -mutant parental cells and STK11 recovered cells by the ENO3 gene knockdown. Cell proliferation was measured based on the direct cell count in the well. Cell viability was measured using CellTiter Blue assay (see the Materials and Methods section for detail). All y-axes represent percentage of cell proliferation or cell viability divided by an average of negative control. Student’s one-tailed t -test was used to test statistical significance. Error bars indicate SD.

    Article Snippet: For siRNA transfection, we experimented with 10 nmol/L target siRNAs of NC (negative control), PLK1 (positive control), and ENO3 (Thermo Fisher Scientific, On-Target Plus SmartPool; GE Dharmacon, USA) ( ).

    Techniques: Mutagenesis, Cell Counting, CtB Assay, Negative Control, One-tailed Test

    Expression of ENO gene family in DNA microarray data and qPCR experiment using LUAD cell lines (A) Comparison of ENO family gene expression (ENO1: 201231_s_at, ENO2: 201313_at, and ENO3: 204483_at) between nine STK11 -mutant and 38 STK11 wild-type cell lines in the CCLE dataset. Wilcoxon rank-sum test for ENO3 and Student’s t -test for others were used to test statistical significance. (B) Experimental validation (qPCR) of ENO family gene expression in STK11 wide-type cell lines (H322M, HCC827, and H1975), STK11 -mutant parental cell lines (A549, H23, and H1993) and STK11 -restored cell lines. The expression level of GAPDH was observed to be varied within SD

    Journal: Molecules and Cells

    Article Title: Overexpression and Selective Anticancer Efficacy of ENO3 in STK11 Mutant Lung Cancers

    doi: 10.14348/molcells.2019.0099

    Figure Lengend Snippet: Expression of ENO gene family in DNA microarray data and qPCR experiment using LUAD cell lines (A) Comparison of ENO family gene expression (ENO1: 201231_s_at, ENO2: 201313_at, and ENO3: 204483_at) between nine STK11 -mutant and 38 STK11 wild-type cell lines in the CCLE dataset. Wilcoxon rank-sum test for ENO3 and Student’s t -test for others were used to test statistical significance. (B) Experimental validation (qPCR) of ENO family gene expression in STK11 wide-type cell lines (H322M, HCC827, and H1975), STK11 -mutant parental cell lines (A549, H23, and H1993) and STK11 -restored cell lines. The expression level of GAPDH was observed to be varied within SD

    Article Snippet: For siRNA transfection, we experimented with 10 nmol/L target siRNAs of NC (negative control), PLK1 (positive control), and ENO3 (Thermo Fisher Scientific, On-Target Plus SmartPool; GE Dharmacon, USA) ( ).

    Techniques: Expressing, Microarray, Real-time Polymerase Chain Reaction, Mutagenesis

    Increased expression levels of ENO1 in hypertensive APAH patients and experimental PH animal lungs. a PASMC isolated from patients with IPAH or APAH and control donors were lysed and subjected to western blotting to measure the level of enolases. b Normalized quantification of proteins demonstrates the expression level of ENO1, ENO2, and ENO3 in PASMC isolated from patients with IPAH or APAH and control donors ( n = 6 per group, * P = 0.0223). c mRNA levels of ENO1, ENO2, and ENO3 in PASMC from patients with PAH and the control donors. d Immunohistochemistry of ENO1 in lung tissue sections of patients with PAH and the control donors. Brown color indicates the staining of ENO1. Black arrows indicate the elevated of ENO1 staining in the PASMC layer of the vessels (scale bars, 100 μm). e Whole lung tissues were isolated from hypoxia-induced PH mice and western blotting was used to measure the level of enolases. f Normalized quantification of proteins demonstrates the expression level of enolases in the whole lung tissue of hypoxia-induced PH mice ( n = 4 per group, * P = 0.0263). g mRNA levels of enolase in the whole lung tissue of hypoxia-induced PH mice ( n = 3–6, P

    Journal: Nature Communications

    Article Title: Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway

    doi: 10.1038/s41467-018-06376-x

    Figure Lengend Snippet: Increased expression levels of ENO1 in hypertensive APAH patients and experimental PH animal lungs. a PASMC isolated from patients with IPAH or APAH and control donors were lysed and subjected to western blotting to measure the level of enolases. b Normalized quantification of proteins demonstrates the expression level of ENO1, ENO2, and ENO3 in PASMC isolated from patients with IPAH or APAH and control donors ( n = 6 per group, * P = 0.0223). c mRNA levels of ENO1, ENO2, and ENO3 in PASMC from patients with PAH and the control donors. d Immunohistochemistry of ENO1 in lung tissue sections of patients with PAH and the control donors. Brown color indicates the staining of ENO1. Black arrows indicate the elevated of ENO1 staining in the PASMC layer of the vessels (scale bars, 100 μm). e Whole lung tissues were isolated from hypoxia-induced PH mice and western blotting was used to measure the level of enolases. f Normalized quantification of proteins demonstrates the expression level of enolases in the whole lung tissue of hypoxia-induced PH mice ( n = 4 per group, * P = 0.0263). g mRNA levels of enolase in the whole lung tissue of hypoxia-induced PH mice ( n = 3–6, P

    Article Snippet: The following primary antibodies were used in this study: α-Tubulin (Cat#T5168, 1:2000, Sigma-Aldrich, St. Louis, MO), β-actin (Cat#A2228, 1:2000, Sigma-Aldrich, St. Louis, MO), α-SMA (Cat#A5228, 1:1000, Sigma-Aldrich, St. Louis, MO), Calponin (Cat#C2687, 1:1000, Sigma-Aldrich, St. Louis, MO), Myocardin (Cat#MAB4028, 1:1000, R & D Systems, Minneapolis, MN), SM22α (Cat#ab10135, 1:1000, Abcam, Cambridge, MA), PCNA (Cat#10205–2-AP, 1:1000, Proteintech Group, Chicago, IL), ENO1 (Cat#3810, 1:1000, Cell Signaling Technology, Danvers, MA), PARP (Cat#9542, 1:1000, Cell Signaling Technology, Danvers, MA), Cleaved-PARP (Cat#5625, 1:1000, Cell Signaling Technology, Danvers, MA), Caspase 3 (Cat#9665, 1:1000, Cell Signaling Technology, Danvers, MA), Cleaved-Caspase 3 (Cat#9664, 1:1000, Cell Signaling Technology, Danvers, MA), Caspase 9 (Cat#9508, 1:1000, Cell Signaling Technology, Danvers, MA), Cleaved-Caspase 9 (Cat#7237, 1:1000, Cell Signaling Technology, Danvers, MA), p-PRAS40 (Cat#13175, 1:1000, Cell Signaling Technology, Danvers, MA), PRAS40 (Cat#2691, 1:1000, Cell Signaling Technology, Danvers, MA), p-Akt (T308) (Cat#13038, 1:1000, Cell Signaling Technology, Danvers, MA), p-Akt (S473) (Cat#4060, 1:1000, Cell Signaling Technology, Danvers, MA), Akt (Cat#4691, 1:1000, Cell Signaling Technology, Danvers, MA), p-GSK3β (Cat#5558, 1:1000, Cell Signaling Technology, Danvers, MA), GSK3β (Cat#12456, 1:1000, Cell Signaling Technology, Danvers, MA), p-ACC (Cat#11818, 1:1000, Cell Signaling Technology, Danvers, MA), ACC (Cat#3676, 1:1000, Cell Signaling Technology, Danvers, MA), p-AMPKα (T172) (Cat#2535, 1:1000, Cell Signaling Technology, Danvers, MA), AMPKα (Cat#5831, 1:1000, Cell Signaling Technology, Danvers, MA), AMPKα1 (Cat#07-350, 1:1000, Upstate Biotechnology, Fairport, NY), AMPKα2 (Cat#07-363, 1:1000, Upstate Biotechnology, Fairport, NY), MHC (Cat#sc-376157, 1:1000, Santa Cruz Biotechnology, Santa Cruz, CA), ENO2 (Cat#sc-71047, 1:1000, Santa Cruz Biotechnology, Santa Cruz, CA), ENO3 (Cat#sc-100811, 1:1000, Santa Cruz Biotechnology, Santa Cruz, CA), and HIF1α (Cat#610958, 1:1000, BD Biosciences, San Jose, CA).

    Techniques: Expressing, Isolation, Western Blot, Immunohistochemistry, Staining, Mouse Assay

    Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , Eno3 , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p

    Journal: Stem Cell Research & Therapy

    Article Title: Pax7 as molecular switch regulating early and advanced stages of myogenic mouse ESC differentiation in teratomas

    doi: 10.1186/s13287-020-01742-3

    Figure Lengend Snippet: Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , Eno3 , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p

    Article Snippet: RNA isolation and qPCR mRNA was isolated from teratomas, using mirVana Isolation Kit (Thermo Fisher Scientific). mRNA analysis reverse transcription was performed using 1 μg total RNA and RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instruction. qPCR was performed using specific TaqMan® probes: Mm02019550_s1 (Nanog ), Mm00435493_m1 (Pax3 ), Mm00435125_m1 (Myf5 ), Mm00440387_m1 (Myod1 ), Mm00446194_m1 (MyoG ), Mm00483191_m1 (Cdh15 ), Mm01318252_m1 (T encoding Brachyury), Mm00477791_m1 (Nfix ), Mm00468267_m1 (Eno3 ), Mm01321487_m1 (Mck ), Mm00434400_m1 (Itga7 ), Mm01318991_m1 (Mef2a ), Mm00452375_m1 (Nfatc4 ), Mm01332463_m1 (Myh3 ), Mm01332564_m1 (Myh2 ), Mm01319006_g1 (Myh7 ), Mm01332541_m1 (Myh4 ), Mm01226102_m1 (Lama1 ), Mm00493080_m1 (Lamb2 ), Mm01193660_m1 (Lama4 ), Mm01222010_g1 (Lama5 ), Mm01248771_m1 (RbFox3 ), Mm00446859_m1 (Otx2 ), Mm01205647_g1 (Actb ), and the TaqMan Gene Expression Master Mix (Thermo Fisher Scientific).

    Techniques: Expressing, Marker, Western Blot, Two Tailed Test

    Amplified fragment length polymorphic complementary DNA (AFLP-cDNA) display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and Mse I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.

    Journal: Methods in enzymology

    Article Title: Methods for Genome-Wide Analysis of Gene Expression Changes in Polyploids

    doi: 10.1016/S0076-6879(05)95030-1

    Figure Lengend Snippet: Amplified fragment length polymorphic complementary DNA (AFLP-cDNA) display analysis of gene expression. (A) A simple diagram of the AFLP-cDNA display procedures including messenger RNA (mRNA) isolation, cDNA synthesis, restriction digestion, ligation, preselective and selective amplification, and gel electrophoresis. The detailed protocols are described in the text. Only a few hypothetical fragments are shown to illustrate how Eco R I (GAATTC)- and Mse I (TTAA)–digested fragments are ligated and amplified during the analysis. (B) AFLP-cDNA display analysis using leaves of Arabidopsis thaliana diploid (At2), autotetraploid (At4), Arabidopsis arenosa (Aa), and generations 2–5 (S2-5) of new allotetraploids, and a natural Arabidopsis suecica ) are not shown because of space limitations.

    Article Snippet: To digest cDNA, add 5 μ l of 5× reaction buffer, about 18 μ l of cDNA (~250 ng), 2 μ l of Eco R I/ Mse I (1.25 U/ μ l each) as supplied by the manufacturer (Invitrogen) to a final volume of 25 μ l. Mix gently, centrifuge briefly, and incubate the reaction at 37° for at least 2 h. The reaction is inactivated by heating at 70° for 15 min.

    Techniques: Amplification, Expressing, Isolation, Ligation, Nucleic Acid Electrophoresis