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    New England Biolabs nebnext poly a mrna magnetic isolation module
    Electropherograms from five embryos of soybean seeds harvested in 1994 (stored 23 years, orange dashed lines) or 2015 (stored 2 years, green solid lines). (A) Total RNA from each sample. The differences in peak heights in 1994H relative to 2015H samples indicate RNA degradation. (B) DNAse-treated, <t>poly-A</t> RNA. The most abundant mRNAs are smaller in 1994H seeds than 2015H seeds.
    Nebnext Poly A Mrna Magnetic Isolation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext poly a mrna magnetic isolation module/product/New England Biolabs
    Average 99 stars, based on 4235 article reviews
    Price from $9.99 to $1999.99
    nebnext poly a mrna magnetic isolation module - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs mrna magnetic isolation module kit
    Electropherograms from five embryos of soybean seeds harvested in 1994 (stored 23 years, orange dashed lines) or 2015 (stored 2 years, green solid lines). (A) Total RNA from each sample. The differences in peak heights in 1994H relative to 2015H samples indicate RNA degradation. (B) DNAse-treated, <t>poly-A</t> RNA. The most abundant mRNAs are smaller in 1994H seeds than 2015H seeds.
    Mrna Magnetic Isolation Module Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrna magnetic isolation module kit/product/New England Biolabs
    Average 99 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    mrna magnetic isolation module kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Electropherograms from five embryos of soybean seeds harvested in 1994 (stored 23 years, orange dashed lines) or 2015 (stored 2 years, green solid lines). (A) Total RNA from each sample. The differences in peak heights in 1994H relative to 2015H samples indicate RNA degradation. (B) DNAse-treated, poly-A RNA. The most abundant mRNAs are smaller in 1994H seeds than 2015H seeds.

    Journal: Journal of Experimental Botany

    Article Title: Exploring the fate of mRNA in aging seeds: protection, destruction, or slow decay?

    doi: 10.1093/jxb/ery215

    Figure Lengend Snippet: Electropherograms from five embryos of soybean seeds harvested in 1994 (stored 23 years, orange dashed lines) or 2015 (stored 2 years, green solid lines). (A) Total RNA from each sample. The differences in peak heights in 1994H relative to 2015H samples indicate RNA degradation. (B) DNAse-treated, poly-A RNA. The most abundant mRNAs are smaller in 1994H seeds than 2015H seeds.

    Article Snippet: The NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA) was used to enrich for mRNA according to the manufacturer’s protocol.

    Techniques:

    This schematic illustrates the sample (auburn rectangle) and library (blue rectangle) preparation workflow to generate the libraries that were loaded on the Illumina sequencer. ( a ) For B. malayi and A. fumigatus , a poly(A)-selected sample was created from an aliquot of total RNA that was used to create a poly(A)-selected library. ( b ) The B. malayi or A. fumigatus AgSS baits were subsequently used to capture the targeted RNA from poly(A)-selected libraries. ( c ) For AgSS-enriched w Bm libraries, an RNA library was constructed from an aliquot of total RNA that underwent targeted enrichment with the Wolbachia AgSS baits. Unlike the eukaryotic enrichments, the bacterial AgSS capture is performed on total RNA. For a limited number of libraries described in the text, an RNA library was constructed from an aliquot of total RNA (i.e. without poly(A)-enrichment) that underwent targeted enrichment with the Brugia AgSS baits. ( d ) For poly(A)/rRNA-depleted libraries enriched for w Bm, an aliquot of total RNA from either mosquito thoraces or adult nematodes was enriched for bacterial mRNA by removing Gram-negative and human rRNAs with two RiboZero removal kits and polyadenylated RNAs with DynaBeads.

    Journal: Scientific Reports

    Article Title: Targeted enrichment outperforms other enrichment techniques and enables more multi-species RNA-Seq analyses

    doi: 10.1038/s41598-018-31420-7

    Figure Lengend Snippet: This schematic illustrates the sample (auburn rectangle) and library (blue rectangle) preparation workflow to generate the libraries that were loaded on the Illumina sequencer. ( a ) For B. malayi and A. fumigatus , a poly(A)-selected sample was created from an aliquot of total RNA that was used to create a poly(A)-selected library. ( b ) The B. malayi or A. fumigatus AgSS baits were subsequently used to capture the targeted RNA from poly(A)-selected libraries. ( c ) For AgSS-enriched w Bm libraries, an RNA library was constructed from an aliquot of total RNA that underwent targeted enrichment with the Wolbachia AgSS baits. Unlike the eukaryotic enrichments, the bacterial AgSS capture is performed on total RNA. For a limited number of libraries described in the text, an RNA library was constructed from an aliquot of total RNA (i.e. without poly(A)-enrichment) that underwent targeted enrichment with the Brugia AgSS baits. ( d ) For poly(A)/rRNA-depleted libraries enriched for w Bm, an aliquot of total RNA from either mosquito thoraces or adult nematodes was enriched for bacterial mRNA by removing Gram-negative and human rRNAs with two RiboZero removal kits and polyadenylated RNAs with DynaBeads.

    Article Snippet: When targeting eukaryotic mRNA, polyadenylated RNA was isolated using the NEBNext Poly(A) mRNA magnetic isolation module.

    Techniques: Construct

    An OSN lineage-specific driver. a Top row: developmental expression of the nonimmortalized GMR82D08-GAL4 (hereafter, at1 driver) using a myr:GFP reporter (green) in the antennal disc SOPs (region marked by α-Dac (blue)) during late larval/early pupal stages. Bottom row: the at1 driver is expressed in the daughter cells of these SOPs in the developing pupal antenna but progressively loses its expression from 20 h APF as OSNs differentiate (visualized with the neuronal marker α-Elav (magenta)). Scale bar = 20 µm in this and other panels. b Immortalization of the at1 driver reveals labeling of clusters of cells in the adult antenna by an rCD2:GFP reporter (green). RNA fluorescence in situ hybridization demonstrates that a single cell within each cluster (arrowheads in the inset images) expresses Or67d mRNA (magenta). c Representative example of a single sensillum in the adult antenna labeled by the immortalized at1 driver, viewed at three focal planes. There is a single Or67d mRNA-positive OSN (cell 1, arrowhead), flanked by four non-neuronal support cells (cells 2–5). d

    Journal: Nature Communications

    Article Title: Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system

    doi: 10.1038/s41467-019-08345-4

    Figure Lengend Snippet: An OSN lineage-specific driver. a Top row: developmental expression of the nonimmortalized GMR82D08-GAL4 (hereafter, at1 driver) using a myr:GFP reporter (green) in the antennal disc SOPs (region marked by α-Dac (blue)) during late larval/early pupal stages. Bottom row: the at1 driver is expressed in the daughter cells of these SOPs in the developing pupal antenna but progressively loses its expression from 20 h APF as OSNs differentiate (visualized with the neuronal marker α-Elav (magenta)). Scale bar = 20 µm in this and other panels. b Immortalization of the at1 driver reveals labeling of clusters of cells in the adult antenna by an rCD2:GFP reporter (green). RNA fluorescence in situ hybridization demonstrates that a single cell within each cluster (arrowheads in the inset images) expresses Or67d mRNA (magenta). c Representative example of a single sensillum in the adult antenna labeled by the immortalized at1 driver, viewed at three focal planes. There is a single Or67d mRNA-positive OSN (cell 1, arrowhead), flanked by four non-neuronal support cells (cells 2–5). d

    Article Snippet: From 100 ng total RNA, mRNA was isolated with the NEBNext Poly(A) mRNA Magnetic Isolation Module.

    Techniques: Expressing, Marker, Labeling, Fluorescence, In Situ Hybridization

    Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. a The relative mRNA level of DLK in infected cells was analyzed by quantitative RT-PCR, normalized to three housekeeping genes and calculated with the ΔΔ C T method. The value of DLK mRNA expression in control cells (pLKO.1) was arbitrarily set to 1. Data are the mean ± SEM (error bars) from three independent experiments carried out in triplicate. ****, p

    Journal: Neural Development

    Article Title: Dual leucine zipper kinase regulates expression of axon guidance genes in mouse neuronal cells

    doi: 10.1186/s13064-016-0068-8

    Figure Lengend Snippet: Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. a The relative mRNA level of DLK in infected cells was analyzed by quantitative RT-PCR, normalized to three housekeeping genes and calculated with the ΔΔ C T method. The value of DLK mRNA expression in control cells (pLKO.1) was arbitrarily set to 1. Data are the mean ± SEM (error bars) from three independent experiments carried out in triplicate. ****, p

    Article Snippet: For all samples, RNA integrity numbers were sufficiently high ( > 9.5) to perform mRNA sequencing. mRNA was purified from 5 μg of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490, New England Biolabs, Whitby, Ontario) following manufacturer’s instructions. cDNA libraries were then prepared with 25 ng mRNA of each sample using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (#E7420, New England Biolabs) and barcoded by PCR for subsequent multiplexed sequencing.

    Techniques: Infection, Plasmid Preparation, Expressing, Selection, RNA Extraction, Quantitative RT-PCR

    Validation of RNA-seq data by qRT-PCR and Western blot analyses. a The relative mRNA level of DLK and axon guidance genes in infected cells was analyzed by qRT-PCR, normalized to three housekeeping genes and calculated with the ΔΔ C T method. The value of mRNA expression for each gene in control cells (pLKO.1) was arbitrarily set to 1. Data are the mean ± SEM (error bars) from three independent experiments carried out in triplicate. *, p

    Journal: Neural Development

    Article Title: Dual leucine zipper kinase regulates expression of axon guidance genes in mouse neuronal cells

    doi: 10.1186/s13064-016-0068-8

    Figure Lengend Snippet: Validation of RNA-seq data by qRT-PCR and Western blot analyses. a The relative mRNA level of DLK and axon guidance genes in infected cells was analyzed by qRT-PCR, normalized to three housekeeping genes and calculated with the ΔΔ C T method. The value of mRNA expression for each gene in control cells (pLKO.1) was arbitrarily set to 1. Data are the mean ± SEM (error bars) from three independent experiments carried out in triplicate. *, p

    Article Snippet: For all samples, RNA integrity numbers were sufficiently high ( > 9.5) to perform mRNA sequencing. mRNA was purified from 5 μg of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490, New England Biolabs, Whitby, Ontario) following manufacturer’s instructions. cDNA libraries were then prepared with 25 ng mRNA of each sample using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (#E7420, New England Biolabs) and barcoded by PCR for subsequent multiplexed sequencing.

    Techniques: RNA Sequencing Assay, Quantitative RT-PCR, Western Blot, Infection, Expressing

    The abundance of snoRNAs relative to the host mRNA in which they are encoded depends on the type of snoRNA and the function of the host genes. ( A , B ) Scatter plots illustrating the relationship between the abundance of box C/D ( A ) and H/ACA ( B ) snoRNAs and the protein-coding RNA produced from their host genes, as determined by FRT. The function of the different host genes is indicated in the legend at the bottom . RP indicates ribosomal protein. The dashed boxes indicate area with the most visible difference between C/D and H/ACA snoRNA.

    Journal: RNA

    Article Title: Simultaneous sequencing of coding and noncoding RNA reveals a human transcriptome dominated by a small number of highly expressed noncoding genes

    doi: 10.1261/rna.064493.117

    Figure Lengend Snippet: The abundance of snoRNAs relative to the host mRNA in which they are encoded depends on the type of snoRNA and the function of the host genes. ( A , B ) Scatter plots illustrating the relationship between the abundance of box C/D ( A ) and H/ACA ( B ) snoRNAs and the protein-coding RNA produced from their host genes, as determined by FRT. The function of the different host genes is indicated in the legend at the bottom . RP indicates ribosomal protein. The dashed boxes indicate area with the most visible difference between C/D and H/ACA snoRNA.

    Article Snippet: The RNA used for FAV and FRV sequencing was isolated and purified from 5 µg DNA-free total RNA extracted using either a NEBNext Poly(A) mRNA Isolation Module (New England Biolabs) in the case of FAV sequencing or Ribo-Zero Gold (Illumina) in the case of FRV, according to the manufacturers’ protocol.

    Techniques: Produced