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  • 93
    ATCC poly a tail
    Poly A Tail, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher poly a tail length assay
    Poly A Tail Length Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore mrna
    FIV budding is not restricted by tetherin in domestic cat cells. Lentiviral vectors were used to express tetherin stably, followed by single-cell cloning and confirmation of expression by immunoblotting for the HA tag (A) and <t>qRT-PCR</t> for BST-2 <t>mRNA</t> transcripts
    Mrna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Illumina Inc messenger rna mrna
    <t>mRNA</t> levels in bas1 and ino4 mutants. (A and B) Comparisons of <t>RNA-seq</t> read counts (in fragments per kilobase per million mapped reads, FPKM, log2-transformed) between wild type and TF mutants for all annotated genes. (C and D) Proportion of genes that
    Messenger Rna Mrna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc sv40 poly a tail
    <t>mRNA</t> levels in bas1 and ino4 mutants. (A and B) Comparisons of <t>RNA-seq</t> read counts (in fragments per kilobase per million mapped reads, FPKM, log2-transformed) between wild type and TF mutants for all annotated genes. (C and D) Proportion of genes that
    Sv40 Poly A Tail, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore messenger rna mrna
    <t>mRNA</t> levels in bas1 and ino4 mutants. (A and B) Comparisons of <t>RNA-seq</t> read counts (in fragments per kilobase per million mapped reads, FPKM, log2-transformed) between wild type and TF mutants for all annotated genes. (C and D) Proportion of genes that
    Messenger Rna Mrna, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TriLink zfn messenger rna mrna
    Hematopoietic recovery following infusion of <t>ZFN</t> <t>mRNA-electroporated</t> HSPCs.
    Zfn Messenger Rna Mrna, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher messenger rna mrna
    Hematopoietic recovery following infusion of <t>ZFN</t> <t>mRNA-electroporated</t> HSPCs.
    Messenger Rna Mrna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore poly a tail addition
    Hematopoietic recovery following infusion of <t>ZFN</t> <t>mRNA-electroporated</t> HSPCs.
    Poly A Tail Addition, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    FIV budding is not restricted by tetherin in domestic cat cells. Lentiviral vectors were used to express tetherin stably, followed by single-cell cloning and confirmation of expression by immunoblotting for the HA tag (A) and qRT-PCR for BST-2 mRNA transcripts

    Journal: Journal of Virology

    Article Title: Feline Immunodeficiency Virus Envelope Glycoproteins Antagonize Tetherin through a Distinctive Mechanism That Requires Virion Incorporation

    doi: 10.1128/JVI.03814-13

    Figure Lengend Snippet: FIV budding is not restricted by tetherin in domestic cat cells. Lentiviral vectors were used to express tetherin stably, followed by single-cell cloning and confirmation of expression by immunoblotting for the HA tag (A) and qRT-PCR for BST-2 mRNA transcripts

    Article Snippet: A table that details all oligonucleotides used in this work is available on request. cDNAs corresponding to fcTetherinLF , accession no. , and fcTetherinSF , accession no. DDBJ , were amplified by reverse transcriptase PCR (RT-PCR) from mRNA of feline PBMCs pretreated with 1,000 U/ml rat IFN-α (Sigma).

    Techniques: Stable Transfection, Clone Assay, Expressing, Quantitative RT-PCR

    vGPCR activates YAP/TAZ. (a) vGPCR expression increases YAP and TAZ protein levels. MCF10A, HEK293A, and SVEC cells stably expressing either the vector control or HA-vGPCR were serum-starved for 12 hours before immunoblotting analysis. (b) vGPCR stabilizes YAP/TAZ. HEK293A cells stably expressing vGPCR were treated with cycloheximide for the indicated time (hours: hr). (c) vGPCR induces YAP/TAZ nuclear localization. HEK293A cells overexpressing either control (CTL) or vGPCR were serum-starved for 12 hours. YAP/TAZ subcellular localization were determined by immunofluorescence staining for YAP/TAZ (red), TAZ (green), along with DAPI for DNA (blue). (d) vGPCR activates a YAP/TAZ reporter. Control (CTL), vGPCR, or positive control LPAR plasmids were co-transfected with a 5 X UAS-luciferase reporter, Renilla and Gal4-TEAD4 into HEK293A cell. 24 hours after transfection, cells were serum starved for 12 hours and luciferase activity was measured and quantified by normalization to the co-transfected Renilla. (e) vGPCR induces expression of YAP/TAZ target genes. mRNA levels of indicated YAP/TAZ target genes was measured in stably expressing control (CTL) and vGPCR cells following 12 hour starvation.

    Journal: Oncogene

    Article Title: Kaposi sarcoma-associated herpesvirus promotes tumorigenesis by modulating the Hippo pathway

    doi: 10.1038/onc.2014.281

    Figure Lengend Snippet: vGPCR activates YAP/TAZ. (a) vGPCR expression increases YAP and TAZ protein levels. MCF10A, HEK293A, and SVEC cells stably expressing either the vector control or HA-vGPCR were serum-starved for 12 hours before immunoblotting analysis. (b) vGPCR stabilizes YAP/TAZ. HEK293A cells stably expressing vGPCR were treated with cycloheximide for the indicated time (hours: hr). (c) vGPCR induces YAP/TAZ nuclear localization. HEK293A cells overexpressing either control (CTL) or vGPCR were serum-starved for 12 hours. YAP/TAZ subcellular localization were determined by immunofluorescence staining for YAP/TAZ (red), TAZ (green), along with DAPI for DNA (blue). (d) vGPCR activates a YAP/TAZ reporter. Control (CTL), vGPCR, or positive control LPAR plasmids were co-transfected with a 5 X UAS-luciferase reporter, Renilla and Gal4-TEAD4 into HEK293A cell. 24 hours after transfection, cells were serum starved for 12 hours and luciferase activity was measured and quantified by normalization to the co-transfected Renilla. (e) vGPCR induces expression of YAP/TAZ target genes. mRNA levels of indicated YAP/TAZ target genes was measured in stably expressing control (CTL) and vGPCR cells following 12 hour starvation.

    Article Snippet: The YAP and TAZ shRNAs targeting human and mouse mRNA were purchased from Sigma and used to make retrovirus to create stable cell lines.

    Techniques: Expressing, Stable Transfection, Plasmid Preparation, CTL Assay, Immunofluorescence, Staining, Positive Control, Transfection, Luciferase, Activity Assay

    BCAS2 does not regulate the transcription initiation of Delta but is involved in Delta pre-mRNA splicing. (A) Control of Dl-lacZ (red) in the en > GFP wing disc stained with anti-β-gal antibody. (B) en > GFP , dBCAS2 dsRNA . In the dBCAS2 -depleted posterior compartment, marked by GFP, the expression of Dl-lacZ (red) gives a signal of similar strength as the normal anterior compartment. The expression of GFP and β-galactosidase were merged and displayed in the right panel. Images were taken by confocal microscopy, scale bar, 50 μm. (C). RNA expression of β-galactosidase. RNAs were extracted from wing discs of third instar larvae and subjected to RT-PCR to confirm the RNA expression of β-galactosidase driven by Dl promoter in Act > dBCAS2 dsRNA (lane 2) compared with the control (lane 1). The internal control, rp49 . (D) Schematic diagram of primer design for detecting the intron-containing precursor mRNA (upper) and mRNA of Delta (lower). Primers, exons and introns are denoted with arrowheads, boxes and lines, respectively. (E) Coexpression of dBCAS2 and dBCAS2 dsRNA in larvae can rescue the phenotypes of mRNA decrease and pre-mRNA accumulation caused by dBCAS2 dsRNA . The pre-mRNA and mRNA of Delta were analyzed by quantitative RT-PCR and described in the Materials and Methods. Each genotype was under the control of Act5c-GAL4 driver. White bar: Act5c > +; black bar: Act5c > dBCAS2 dsRNA ; gray bar: Act5c > dBCAS2 dsRNA , dBCAS2 . Data are shown as means and SD relative to the controls from three independent experiments. The P-values was measured by the Student’s t-test. * p

    Journal: PLoS ONE

    Article Title: BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development

    doi: 10.1371/journal.pone.0130706

    Figure Lengend Snippet: BCAS2 does not regulate the transcription initiation of Delta but is involved in Delta pre-mRNA splicing. (A) Control of Dl-lacZ (red) in the en > GFP wing disc stained with anti-β-gal antibody. (B) en > GFP , dBCAS2 dsRNA . In the dBCAS2 -depleted posterior compartment, marked by GFP, the expression of Dl-lacZ (red) gives a signal of similar strength as the normal anterior compartment. The expression of GFP and β-galactosidase were merged and displayed in the right panel. Images were taken by confocal microscopy, scale bar, 50 μm. (C). RNA expression of β-galactosidase. RNAs were extracted from wing discs of third instar larvae and subjected to RT-PCR to confirm the RNA expression of β-galactosidase driven by Dl promoter in Act > dBCAS2 dsRNA (lane 2) compared with the control (lane 1). The internal control, rp49 . (D) Schematic diagram of primer design for detecting the intron-containing precursor mRNA (upper) and mRNA of Delta (lower). Primers, exons and introns are denoted with arrowheads, boxes and lines, respectively. (E) Coexpression of dBCAS2 and dBCAS2 dsRNA in larvae can rescue the phenotypes of mRNA decrease and pre-mRNA accumulation caused by dBCAS2 dsRNA . The pre-mRNA and mRNA of Delta were analyzed by quantitative RT-PCR and described in the Materials and Methods. Each genotype was under the control of Act5c-GAL4 driver. White bar: Act5c > +; black bar: Act5c > dBCAS2 dsRNA ; gray bar: Act5c > dBCAS2 dsRNA , dBCAS2 . Data are shown as means and SD relative to the controls from three independent experiments. The P-values was measured by the Student’s t-test. * p

    Article Snippet: In vivo splicing assays For the detection of mRNA and pre-mRNA in Drosophila , total RNA from imaginal wing discs in 20 third instar larvae was isolated by using TRI reagent (Sigma-Aldrich).

    Techniques: Staining, Expressing, Confocal Microscopy, RNA Expression, Reverse Transcription Polymerase Chain Reaction, Activated Clotting Time Assay, Quantitative RT-PCR

    mRNA levels in bas1 and ino4 mutants. (A and B) Comparisons of RNA-seq read counts (in fragments per kilobase per million mapped reads, FPKM, log2-transformed) between wild type and TF mutants for all annotated genes. (C and D) Proportion of genes that

    Journal: Genetics

    Article Title: High-Resolution Global Analysis of the Influences of Bas1 and Ino4 Transcription Factors on Meiotic DNA Break Distributions in Saccharomyces cerevisiae

    doi: 10.1534/genetics.115.178293

    Figure Lengend Snippet: mRNA levels in bas1 and ino4 mutants. (A and B) Comparisons of RNA-seq read counts (in fragments per kilobase per million mapped reads, FPKM, log2-transformed) between wild type and TF mutants for all annotated genes. (C and D) Proportion of genes that

    Article Snippet: Messenger RNA (mRNA) was enriched by poly-A selection and sequenced using Illumina HiSeq in the Genomics Resources Core Facility of Weill Cornell Medical College.

    Techniques: RNA Sequencing Assay, Transformation Assay

    Diagram illustrating the experimental design and workflow for RNA-seq data analysis. Briefly, tissues from 2 to 3-month-old C57BL/6 conventional (CV) and germ-free (GF) mice were harvested as described previously [ 29 ]. Total RNAs were extracted from each organ ( n = 3 per enterotype per organ). The cDNA libraries were prepared using the poly-A tail selection method and were sequenced using an Illumina HiSeq2000 sequencer (2 × 50 bp paired-end). Fastq files were quality-checked with FastQC and mapped to the mouse reference genome (mm10) using HISAT. The sequence alignment mapping (SAM) files were converted to binary alignment mapping (BAM) files and sorted using SAMtools. The sorted BAM files were subjected to Cufflinks to determine the transcript abundance. Specifically, the transcript abundance of lncRNAs and PCGs was estimated using the mouse NONCODE 2016 lncRNA and UCSC mm10 PCG reference gene transfer format (GTF) files, respectively. The differentially expressed lncRNAs and PCGs were determined by Cuffdiff between CV and GF mice for each organ (FDR-BH

    Journal: BMC Genomics

    Article Title: Coordinate regulation of long non-coding RNAs and protein-coding genes in germ-free mice

    doi: 10.1186/s12864-018-5235-3

    Figure Lengend Snippet: Diagram illustrating the experimental design and workflow for RNA-seq data analysis. Briefly, tissues from 2 to 3-month-old C57BL/6 conventional (CV) and germ-free (GF) mice were harvested as described previously [ 29 ]. Total RNAs were extracted from each organ ( n = 3 per enterotype per organ). The cDNA libraries were prepared using the poly-A tail selection method and were sequenced using an Illumina HiSeq2000 sequencer (2 × 50 bp paired-end). Fastq files were quality-checked with FastQC and mapped to the mouse reference genome (mm10) using HISAT. The sequence alignment mapping (SAM) files were converted to binary alignment mapping (BAM) files and sorted using SAMtools. The sorted BAM files were subjected to Cufflinks to determine the transcript abundance. Specifically, the transcript abundance of lncRNAs and PCGs was estimated using the mouse NONCODE 2016 lncRNA and UCSC mm10 PCG reference gene transfer format (GTF) files, respectively. The differentially expressed lncRNAs and PCGs were determined by Cuffdiff between CV and GF mice for each organ (FDR-BH

    Article Snippet: The complementary DNA (cDNA) libraries were constructed from total RNA samples using a TruSeq RNA Sample Prep Kit with poly-A tail selection (Illumina, San Diego, CA).

    Techniques: RNA Sequencing Assay, Mouse Assay, Selection, Sequencing

    Hematopoietic recovery following infusion of ZFN mRNA-electroporated HSPCs.

    Journal: Blood

    Article Title: Long-term multilineage engraftment of autologous genome-edited hematopoietic stem cells in nonhuman primates

    doi: 10.1182/blood-2015-09-672337

    Figure Lengend Snippet: Hematopoietic recovery following infusion of ZFN mRNA-electroporated HSPCs.

    Article Snippet: ZFN messenger RNA (mRNA) (TriLink BioTechnologies) was added to cells resuspended to 1 × 107 cells/mL in Cytoporation Media T (Harvard Apparatus, Holliston, MA) at a final concentration of 125 μg/mL for each ZFN mRNA.

    Techniques: