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    R&D Systems mrgmb his
    Blocking capacities of RGMb and PD-L2 mAbs and a model for RGMb and PD-L2 interactions. (a–c) The blocking capacity of RGMb mAbs and PD-L2 mAbs was determined by cell conjugation assay. After cells were stained with dyes, (a) <t>300-mRGMb</t> cells were incubated with the indicated concentrations of RGMb mAbs before incubation with 300-mPD-L2 cells; (b) 300-mPD-L2 cells were incubated with the indicated concentrations of PD-L2 mAbs before incubation with 300-mRGMb cells; or (c) before incubation with 300-mPD-1 cells. (d) <t>mRGMb-HIS</t> was preincubated with mAbs or Ig fusion proteins and then added to BMP-4–coated plates. Binding of mRGMb-HIS was detected by anti–penta-HIS-HRP in an ELISA. Similar results were seen with BMP-2 (not depicted). (e) mPD-L2-hIgG or control-hIgG were preincubated alone or with monomeric mRGMb-HIS, and then added to BMP-4–coated plates. Binding of Ig fusion proteins was detected with anti–hIgG-HRP in an ELISA. Similar results were also seen for BMP-2 (not depicted). (f) mNeogenin- or mPD-L2–transfected 300 cells were stained with indicated Ig fusion proteins or control-Ig and analyzed by flow cytometry. (g) Binding of 300-mRGMb cells to 300-mPD-L2 or 300-mNeogenin cells was analyzed by cell conjugation assay. Cells stained with a red dye were incubated with cells stained with a green dye and binding was measured by flow cytometry. (h) Molecular model depicting PDL2–BMP–BMPR–RGMb–neogenin and PD-L2–PD-1 pathways. All data are representative of two or more independent experiments.
    Mrgmb His, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrgmb his/product/R&D Systems
    Average 88 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    mrgmb his - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    87
    R&D Systems recombinant mrgmb his
    Blocking capacities of RGMb and PD-L2 mAbs and a model for RGMb and PD-L2 interactions. (a–c) The blocking capacity of RGMb mAbs and PD-L2 mAbs was determined by cell conjugation assay. After cells were stained with dyes, (a) <t>300-mRGMb</t> cells were incubated with the indicated concentrations of RGMb mAbs before incubation with 300-mPD-L2 cells; (b) 300-mPD-L2 cells were incubated with the indicated concentrations of PD-L2 mAbs before incubation with 300-mRGMb cells; or (c) before incubation with 300-mPD-1 cells. (d) <t>mRGMb-HIS</t> was preincubated with mAbs or Ig fusion proteins and then added to BMP-4–coated plates. Binding of mRGMb-HIS was detected by anti–penta-HIS-HRP in an ELISA. Similar results were seen with BMP-2 (not depicted). (e) mPD-L2-hIgG or control-hIgG were preincubated alone or with monomeric mRGMb-HIS, and then added to BMP-4–coated plates. Binding of Ig fusion proteins was detected with anti–hIgG-HRP in an ELISA. Similar results were also seen for BMP-2 (not depicted). (f) mNeogenin- or mPD-L2–transfected 300 cells were stained with indicated Ig fusion proteins or control-Ig and analyzed by flow cytometry. (g) Binding of 300-mRGMb cells to 300-mPD-L2 or 300-mNeogenin cells was analyzed by cell conjugation assay. Cells stained with a red dye were incubated with cells stained with a green dye and binding was measured by flow cytometry. (h) Molecular model depicting PDL2–BMP–BMPR–RGMb–neogenin and PD-L2–PD-1 pathways. All data are representative of two or more independent experiments.
    Recombinant Mrgmb His, supplied by R&D Systems, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mrgmb his/product/R&D Systems
    Average 87 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    recombinant mrgmb his - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    Image Search Results


    Blocking capacities of RGMb and PD-L2 mAbs and a model for RGMb and PD-L2 interactions. (a–c) The blocking capacity of RGMb mAbs and PD-L2 mAbs was determined by cell conjugation assay. After cells were stained with dyes, (a) 300-mRGMb cells were incubated with the indicated concentrations of RGMb mAbs before incubation with 300-mPD-L2 cells; (b) 300-mPD-L2 cells were incubated with the indicated concentrations of PD-L2 mAbs before incubation with 300-mRGMb cells; or (c) before incubation with 300-mPD-1 cells. (d) mRGMb-HIS was preincubated with mAbs or Ig fusion proteins and then added to BMP-4–coated plates. Binding of mRGMb-HIS was detected by anti–penta-HIS-HRP in an ELISA. Similar results were seen with BMP-2 (not depicted). (e) mPD-L2-hIgG or control-hIgG were preincubated alone or with monomeric mRGMb-HIS, and then added to BMP-4–coated plates. Binding of Ig fusion proteins was detected with anti–hIgG-HRP in an ELISA. Similar results were also seen for BMP-2 (not depicted). (f) mNeogenin- or mPD-L2–transfected 300 cells were stained with indicated Ig fusion proteins or control-Ig and analyzed by flow cytometry. (g) Binding of 300-mRGMb cells to 300-mPD-L2 or 300-mNeogenin cells was analyzed by cell conjugation assay. Cells stained with a red dye were incubated with cells stained with a green dye and binding was measured by flow cytometry. (h) Molecular model depicting PDL2–BMP–BMPR–RGMb–neogenin and PD-L2–PD-1 pathways. All data are representative of two or more independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: Blocking capacities of RGMb and PD-L2 mAbs and a model for RGMb and PD-L2 interactions. (a–c) The blocking capacity of RGMb mAbs and PD-L2 mAbs was determined by cell conjugation assay. After cells were stained with dyes, (a) 300-mRGMb cells were incubated with the indicated concentrations of RGMb mAbs before incubation with 300-mPD-L2 cells; (b) 300-mPD-L2 cells were incubated with the indicated concentrations of PD-L2 mAbs before incubation with 300-mRGMb cells; or (c) before incubation with 300-mPD-1 cells. (d) mRGMb-HIS was preincubated with mAbs or Ig fusion proteins and then added to BMP-4–coated plates. Binding of mRGMb-HIS was detected by anti–penta-HIS-HRP in an ELISA. Similar results were seen with BMP-2 (not depicted). (e) mPD-L2-hIgG or control-hIgG were preincubated alone or with monomeric mRGMb-HIS, and then added to BMP-4–coated plates. Binding of Ig fusion proteins was detected with anti–hIgG-HRP in an ELISA. Similar results were also seen for BMP-2 (not depicted). (f) mNeogenin- or mPD-L2–transfected 300 cells were stained with indicated Ig fusion proteins or control-Ig and analyzed by flow cytometry. (g) Binding of 300-mRGMb cells to 300-mPD-L2 or 300-mNeogenin cells was analyzed by cell conjugation assay. Cells stained with a red dye were incubated with cells stained with a green dye and binding was measured by flow cytometry. (h) Molecular model depicting PDL2–BMP–BMPR–RGMb–neogenin and PD-L2–PD-1 pathways. All data are representative of two or more independent experiments.

    Article Snippet: Alternatively, 10 µg/ml mRGMb-HIS (R & D Systems) or buffer alone was added first to the plates and incubated for 1 h at 37°C.

    Techniques: Blocking Assay, Conjugation Assay, Staining, Incubation, Binding Assay, Enzyme-linked Immunosorbent Assay, Transfection, Flow Cytometry, Cytometry

    RGMb binds to PD-L2, but not PD-L1 or other related molecules. (a and b) mRGMb- or mPD-L2–transfected 300 cells, untransfected 300 cells, and PD-1–transfected Jurkat T cells were stained with the indicated Ig fusion proteins (red) or control-Ig (blue) and analyzed by flow cytometry. MFI, mean fluorescence intensity. (c and d) Binding of mPD-L2-Ig or control-Ig to plates coated with mRGMb-HIS, hRGMb-HIS, mRGMa-HIS or mRGMc-HIS, was analyzed by ELISA. (e) Biacore analysis of the interaction of RGMb with PD-L2 and of PD-1 with PD-L2. (f–k) Cell–cell binding of the indicated transfected cells was analyzed by cell conjugation assay. In f, h, and j, the top panels show the FSC-SSC plots and the bottom panels show the corresponding dot plots. g, i, and k show only the dot plots. All data are representative of 2–11 independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: RGMb binds to PD-L2, but not PD-L1 or other related molecules. (a and b) mRGMb- or mPD-L2–transfected 300 cells, untransfected 300 cells, and PD-1–transfected Jurkat T cells were stained with the indicated Ig fusion proteins (red) or control-Ig (blue) and analyzed by flow cytometry. MFI, mean fluorescence intensity. (c and d) Binding of mPD-L2-Ig or control-Ig to plates coated with mRGMb-HIS, hRGMb-HIS, mRGMa-HIS or mRGMc-HIS, was analyzed by ELISA. (e) Biacore analysis of the interaction of RGMb with PD-L2 and of PD-1 with PD-L2. (f–k) Cell–cell binding of the indicated transfected cells was analyzed by cell conjugation assay. In f, h, and j, the top panels show the FSC-SSC plots and the bottom panels show the corresponding dot plots. g, i, and k show only the dot plots. All data are representative of 2–11 independent experiments.

    Article Snippet: Alternatively, 10 µg/ml mRGMb-HIS (R & D Systems) or buffer alone was added first to the plates and incubated for 1 h at 37°C.

    Techniques: Transfection, Staining, Flow Cytometry, Cytometry, Fluorescence, Binding Assay, Enzyme-linked Immunosorbent Assay, Conjugation Assay

    Blocking capacities of RGMb and PD-L2 mAbs and a model for RGMb and PD-L2 interactions. (a–c) The blocking capacity of RGMb mAbs and PD-L2 mAbs was determined by cell conjugation assay. After cells were stained with dyes, (a) 300-mRGMb cells were incubated with the indicated concentrations of RGMb mAbs before incubation with 300-mPD-L2 cells; (b) 300-mPD-L2 cells were incubated with the indicated concentrations of PD-L2 mAbs before incubation with 300-mRGMb cells; or (c) before incubation with 300-mPD-1 cells. (d) mRGMb-HIS was preincubated with mAbs or Ig fusion proteins and then added to BMP-4–coated plates. Binding of mRGMb-HIS was detected by anti–penta-HIS-HRP in an ELISA. Similar results were seen with BMP-2 (not depicted). (e) mPD-L2-hIgG or control-hIgG were preincubated alone or with monomeric mRGMb-HIS, and then added to BMP-4–coated plates. Binding of Ig fusion proteins was detected with anti–hIgG-HRP in an ELISA. Similar results were also seen for BMP-2 (not depicted). (f) mNeogenin- or mPD-L2–transfected 300 cells were stained with indicated Ig fusion proteins or control-Ig and analyzed by flow cytometry. (g) Binding of 300-mRGMb cells to 300-mPD-L2 or 300-mNeogenin cells was analyzed by cell conjugation assay. Cells stained with a red dye were incubated with cells stained with a green dye and binding was measured by flow cytometry. (h) Molecular model depicting PDL2–BMP–BMPR–RGMb–neogenin and PD-L2–PD-1 pathways. All data are representative of two or more independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: Blocking capacities of RGMb and PD-L2 mAbs and a model for RGMb and PD-L2 interactions. (a–c) The blocking capacity of RGMb mAbs and PD-L2 mAbs was determined by cell conjugation assay. After cells were stained with dyes, (a) 300-mRGMb cells were incubated with the indicated concentrations of RGMb mAbs before incubation with 300-mPD-L2 cells; (b) 300-mPD-L2 cells were incubated with the indicated concentrations of PD-L2 mAbs before incubation with 300-mRGMb cells; or (c) before incubation with 300-mPD-1 cells. (d) mRGMb-HIS was preincubated with mAbs or Ig fusion proteins and then added to BMP-4–coated plates. Binding of mRGMb-HIS was detected by anti–penta-HIS-HRP in an ELISA. Similar results were seen with BMP-2 (not depicted). (e) mPD-L2-hIgG or control-hIgG were preincubated alone or with monomeric mRGMb-HIS, and then added to BMP-4–coated plates. Binding of Ig fusion proteins was detected with anti–hIgG-HRP in an ELISA. Similar results were also seen for BMP-2 (not depicted). (f) mNeogenin- or mPD-L2–transfected 300 cells were stained with indicated Ig fusion proteins or control-Ig and analyzed by flow cytometry. (g) Binding of 300-mRGMb cells to 300-mPD-L2 or 300-mNeogenin cells was analyzed by cell conjugation assay. Cells stained with a red dye were incubated with cells stained with a green dye and binding was measured by flow cytometry. (h) Molecular model depicting PDL2–BMP–BMPR–RGMb–neogenin and PD-L2–PD-1 pathways. All data are representative of two or more independent experiments.

    Article Snippet: Rats were immunized three times via intramuscular and intravenous injection of mRGMb plasmid cDNA , and boosted three times with recombinant mRGMb-HIS (R & D Systems) via i.p. and s.c. injection.

    Techniques: Blocking Assay, Conjugation Assay, Staining, Incubation, Binding Assay, Enzyme-linked Immunosorbent Assay, Transfection, Flow Cytometry, Cytometry

    RGMb binds to PD-L2, but not PD-L1 or other related molecules. (a and b) mRGMb- or mPD-L2–transfected 300 cells, untransfected 300 cells, and PD-1–transfected Jurkat T cells were stained with the indicated Ig fusion proteins (red) or control-Ig (blue) and analyzed by flow cytometry. MFI, mean fluorescence intensity. (c and d) Binding of mPD-L2-Ig or control-Ig to plates coated with mRGMb-HIS, hRGMb-HIS, mRGMa-HIS or mRGMc-HIS, was analyzed by ELISA. (e) Biacore analysis of the interaction of RGMb with PD-L2 and of PD-1 with PD-L2. (f–k) Cell–cell binding of the indicated transfected cells was analyzed by cell conjugation assay. In f, h, and j, the top panels show the FSC-SSC plots and the bottom panels show the corresponding dot plots. g, i, and k show only the dot plots. All data are representative of 2–11 independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: RGMb binds to PD-L2, but not PD-L1 or other related molecules. (a and b) mRGMb- or mPD-L2–transfected 300 cells, untransfected 300 cells, and PD-1–transfected Jurkat T cells were stained with the indicated Ig fusion proteins (red) or control-Ig (blue) and analyzed by flow cytometry. MFI, mean fluorescence intensity. (c and d) Binding of mPD-L2-Ig or control-Ig to plates coated with mRGMb-HIS, hRGMb-HIS, mRGMa-HIS or mRGMc-HIS, was analyzed by ELISA. (e) Biacore analysis of the interaction of RGMb with PD-L2 and of PD-1 with PD-L2. (f–k) Cell–cell binding of the indicated transfected cells was analyzed by cell conjugation assay. In f, h, and j, the top panels show the FSC-SSC plots and the bottom panels show the corresponding dot plots. g, i, and k show only the dot plots. All data are representative of 2–11 independent experiments.

    Article Snippet: Rats were immunized three times via intramuscular and intravenous injection of mRGMb plasmid cDNA , and boosted three times with recombinant mRGMb-HIS (R & D Systems) via i.p. and s.c. injection.

    Techniques: Transfection, Staining, Flow Cytometry, Cytometry, Fluorescence, Binding Assay, Enzyme-linked Immunosorbent Assay, Conjugation Assay