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  • mpc 11  (ATCC)
    92
    ATCC mpc 11
    Analysis of virus spread in myeloma tumors in vivo . (A) <t>MPC-11</t> tumor-bearing mice were injected with a single intravenous dose (10 8 TCID 50 s) of the indicated viruses. Tumors were harvested and sectioned at 24, 48, and 72 h posttreatment and immunohistochemistry (IHC) carried out to detect VSV antigen (red) and cell nuclei (Hoechst/blue). Magnification, ×40. (B) Relative expression of the N gene in the indicated mouse tumor by real-time PCR analysis. The data are the N gene level normalized to the GAPDH RNA level relative to that in tumor tissue and are represented by the mean ± SD ( n = 3).
    Mpc 11, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore mpc 11 cells
    Phosphorylation of PSF during mitosis and apoptosis. HeLa cells were blocked at G1/S by a thymidine block followed by a nocodazole block causing cells to stop at metaphase. (A) Protein extracts were made from untreated and treated cells, and blots were probed with the B92 antibody. (B) Extracts were made from HeLa cells treated with nocodazole for the indicated time periods. (C) The cells were then released from the mitotic block, and extracts were made after a few hours. (D) Mouse <t>MPC-11</t> cells underwent the same treatments. (E) Mitotically blocked HeLa protein extracts were treated with λ protein phosphatase at 30°C for 1 h. (F) HL-60 apoptotic cells were separated from nonapoptotic cells with the use of a Percoll gradient. The shift in the apoptotic cells was compared with mitotic cells. All the shifts observed were reproducible.
    Mpc 11 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Mesoblast Ltd mpcs
    Phosphorylation of PSF during mitosis and apoptosis. HeLa cells were blocked at G1/S by a thymidine block followed by a nocodazole block causing cells to stop at metaphase. (A) Protein extracts were made from untreated and treated cells, and blots were probed with the B92 antibody. (B) Extracts were made from HeLa cells treated with nocodazole for the indicated time periods. (C) The cells were then released from the mitotic block, and extracts were made after a few hours. (D) Mouse <t>MPC-11</t> cells underwent the same treatments. (E) Mitotically blocked HeLa protein extracts were treated with λ protein phosphatase at 30°C for 1 h. (F) HL-60 apoptotic cells were separated from nonapoptotic cells with the use of a Percoll gradient. The shift in the apoptotic cells was compared with mitotic cells. All the shifts observed were reproducible.
    Mpcs, supplied by Mesoblast Ltd, used in various techniques. Bioz Stars score: 91/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore protein repellent mpc mpc
    Phosphorylation of PSF during mitosis and apoptosis. HeLa cells were blocked at G1/S by a thymidine block followed by a nocodazole block causing cells to stop at metaphase. (A) Protein extracts were made from untreated and treated cells, and blots were probed with the B92 antibody. (B) Extracts were made from HeLa cells treated with nocodazole for the indicated time periods. (C) The cells were then released from the mitotic block, and extracts were made after a few hours. (D) Mouse <t>MPC-11</t> cells underwent the same treatments. (E) Mitotically blocked HeLa protein extracts were treated with λ protein phosphatase at 30°C for 1 h. (F) HL-60 apoptotic cells were separated from nonapoptotic cells with the use of a Percoll gradient. The shift in the apoptotic cells was compared with mitotic cells. All the shifts observed were reproducible.
    Protein Repellent Mpc Mpc, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher dynal mpc
    A comparison between the performance of our chip and a <t>Dynal</t> <t>MPC.</t>
    Dynal Mpc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore mpc powder
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Mpc Powder, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher mpc
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Mpc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Sutter Instrument mpc 385
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Mpc 385, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Ancell corporation mpc 11
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Mpc 11, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Sutter Instrument mpc 200 manipulators
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Mpc 200 Manipulators, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Sutter Instrument mpc 325 micromanipulators
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Mpc 325 Micromanipulators, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Nof corporation mpc polymer
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Mpc Polymer, supplied by Nof corporation, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    BioLegend mpc 11
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Mpc 11, supplied by BioLegend, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mpc s
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Mpc S, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher dynal mpc l
    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% <t>MPC-LCFV.</t> Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P
    Dynal Mpc L, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Analysis of virus spread in myeloma tumors in vivo . (A) MPC-11 tumor-bearing mice were injected with a single intravenous dose (10 8 TCID 50 s) of the indicated viruses. Tumors were harvested and sectioned at 24, 48, and 72 h posttreatment and immunohistochemistry (IHC) carried out to detect VSV antigen (red) and cell nuclei (Hoechst/blue). Magnification, ×40. (B) Relative expression of the N gene in the indicated mouse tumor by real-time PCR analysis. The data are the N gene level normalized to the GAPDH RNA level relative to that in tumor tissue and are represented by the mean ± SD ( n = 3).

    Journal: Journal of Virology

    Article Title: Neuroattenuation of Vesicular Stomatitis Virus through Picornaviral Internal Ribosome Entry Sites

    doi: 10.1128/JVI.02984-12

    Figure Lengend Snippet: Analysis of virus spread in myeloma tumors in vivo . (A) MPC-11 tumor-bearing mice were injected with a single intravenous dose (10 8 TCID 50 s) of the indicated viruses. Tumors were harvested and sectioned at 24, 48, and 72 h posttreatment and immunohistochemistry (IHC) carried out to detect VSV antigen (red) and cell nuclei (Hoechst/blue). Magnification, ×40. (B) Relative expression of the N gene in the indicated mouse tumor by real-time PCR analysis. The data are the N gene level normalized to the GAPDH RNA level relative to that in tumor tissue and are represented by the mean ± SD ( n = 3).

    Article Snippet: BHK, Vero, MPC-11, A375, HeLa, 293T, PC3, U87, Hep3B, and MDA MB-321 cells were obtained from American Type Culture Collection (ATCC), Manassas, VA, and were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) in 5% CO2 .

    Techniques: In Vivo, Mouse Assay, Injection, Immunohistochemistry, Expressing, Real-time Polymerase Chain Reaction

    Oncolytic efficacy of IRES-controlled viruses. (A to D) Mice (BALB/c, 4 weeks old) bearing subcutaneous MPC-11 tumors were treated with a single intravenous dose (1 × 10 8 ) of Opti-MEM (A), VSV Δ51 (B), VSV FMDV (C), or (D) VSV HRV . Tumor size was measured by serial caliper measurements. (E) Kaplan-Meier survival curves for the mice from panels A through D. *, P = 0.0009 versus Opti-MEM; **, P = 0.0052 versus Opti-MEM; Δ, P = 0.2263 versus VSV FMDV , P = 0.0466 versus VSV HRV , and P = 0.1883 VSV FMDV versus VSV HRV . (F) Mouse body weight analysis. The average body weight per group throughout the experiment is plotted as the mean ± SD. ‡, no mouse was left after this point.

    Journal: Journal of Virology

    Article Title: Neuroattenuation of Vesicular Stomatitis Virus through Picornaviral Internal Ribosome Entry Sites

    doi: 10.1128/JVI.02984-12

    Figure Lengend Snippet: Oncolytic efficacy of IRES-controlled viruses. (A to D) Mice (BALB/c, 4 weeks old) bearing subcutaneous MPC-11 tumors were treated with a single intravenous dose (1 × 10 8 ) of Opti-MEM (A), VSV Δ51 (B), VSV FMDV (C), or (D) VSV HRV . Tumor size was measured by serial caliper measurements. (E) Kaplan-Meier survival curves for the mice from panels A through D. *, P = 0.0009 versus Opti-MEM; **, P = 0.0052 versus Opti-MEM; Δ, P = 0.2263 versus VSV FMDV , P = 0.0466 versus VSV HRV , and P = 0.1883 VSV FMDV versus VSV HRV . (F) Mouse body weight analysis. The average body weight per group throughout the experiment is plotted as the mean ± SD. ‡, no mouse was left after this point.

    Article Snippet: BHK, Vero, MPC-11, A375, HeLa, 293T, PC3, U87, Hep3B, and MDA MB-321 cells were obtained from American Type Culture Collection (ATCC), Manassas, VA, and were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) in 5% CO2 .

    Techniques: Mouse Assay

    Susceptibility of tumor cells to VSV infection in vitro and in vivo . (a) Propagation of VSV-GFP was quantitated by TCID 50 titrations and fold increase in virus yield (virus output/input) is calculated (n = 3 replicates, mean ± SD). (b) Cell viability in VSV infected cultures was evaluated by the MTS assay at 48 h after infection at the respective MOIs. (c) Representative image of immunohistochemical staining for VSV protein (Alexa-555/red staining) in MPC-11, LM-1 and EMT-6 xenografts at 24 h post intravenous VSV delivery. (d) Red fluorescent 200 nm polystyrene microspheres in the tumors at 2 h after intravenous delivery. (e) Quantitative RT-PCR for VSV nucleocapsid mRNA in the tumors at 2 h or 24 h post virus delivery. Mean ± SD (n = 3 mice per time point). * P ≤ 0.05. Unpaired student t test was used. (f) Abundant and uniform distribution of CD68 cells (Alexa488/green staining) in the tumors. Scale bar represents 100 μm.

    Journal: Scientific Reports

    Article Title: Induction of antiviral genes by the tumor microenvironment confers resistance to virotherapy

    doi: 10.1038/srep02375

    Figure Lengend Snippet: Susceptibility of tumor cells to VSV infection in vitro and in vivo . (a) Propagation of VSV-GFP was quantitated by TCID 50 titrations and fold increase in virus yield (virus output/input) is calculated (n = 3 replicates, mean ± SD). (b) Cell viability in VSV infected cultures was evaluated by the MTS assay at 48 h after infection at the respective MOIs. (c) Representative image of immunohistochemical staining for VSV protein (Alexa-555/red staining) in MPC-11, LM-1 and EMT-6 xenografts at 24 h post intravenous VSV delivery. (d) Red fluorescent 200 nm polystyrene microspheres in the tumors at 2 h after intravenous delivery. (e) Quantitative RT-PCR for VSV nucleocapsid mRNA in the tumors at 2 h or 24 h post virus delivery. Mean ± SD (n = 3 mice per time point). * P ≤ 0.05. Unpaired student t test was used. (f) Abundant and uniform distribution of CD68 cells (Alexa488/green staining) in the tumors. Scale bar represents 100 μm.

    Article Snippet: Cells and viruses EMT-6 murine mammary carcinoma, LLC1 murine Lewis lung carcinoma, MPC-11 murine myeloma, RAW264.7, and J774A.1 murine monocyte/macrophage cells were obtained from the American Type Culture Collection (Manassas, VA).

    Techniques: Infection, In Vitro, In Vivo, MTS Assay, Immunohistochemistry, Staining, Quantitative RT-PCR, Mouse Assay

    Monitoring intratumoral spread of intravenously administered VSV-IFN-NIS Female, 6-10 week old C57Bl6/KaLwRij mice bearing subcutaneous syngeneic 5TGM1 myeloma tumors or Balb/c mice bearing subcutaneous MPC-11 myeloma tumors were treated with a single intravenous (IV) dose of 100ul PBS, or 1×10 8 TCID 50 VSV-mIFNβ-NIS. SPECT-CT imaging was performed at 24h intervals, each image being obtained one hour after intraperitoneal administration of 99m TcO 4 (500μCi). Serial day 1, 2, 3 and 4 SPECT/CT images from one representative animal (right panel) bearing (A) 5TGM1 myeloma or (B) MPC-11 myeloma show rapid radioiodine uptake following virus administration. Radioisotope uptake is seen in the thyroid gland (Th), and stomach (St), with slight excreted radioisotope visible in the bladder (Bl). Tumors from control PBS-treated animals (on left) show only background 99m TcO 4 uptake. Semi-quantitative monitoring of intratumoral virus spread in subcutaneous (C) 5TGM1 and (D) MPC-11 tumor models. SPECT/CT images from n=5 VSV-mIFNβ-NIS-treated and n=2 control (PBS-treated) animals were analyzed to quantify 99m TcO 4 radioisotope uptake by tumors days 1 through 5 following virus therapy. Mean group values are plotted for each timepoint (errors bars indicate SEM)

    Journal: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K

    Article Title: Curative one-shot systemic virotherapy in murine myeloma

    doi: 10.1038/leu.2012.70

    Figure Lengend Snippet: Monitoring intratumoral spread of intravenously administered VSV-IFN-NIS Female, 6-10 week old C57Bl6/KaLwRij mice bearing subcutaneous syngeneic 5TGM1 myeloma tumors or Balb/c mice bearing subcutaneous MPC-11 myeloma tumors were treated with a single intravenous (IV) dose of 100ul PBS, or 1×10 8 TCID 50 VSV-mIFNβ-NIS. SPECT-CT imaging was performed at 24h intervals, each image being obtained one hour after intraperitoneal administration of 99m TcO 4 (500μCi). Serial day 1, 2, 3 and 4 SPECT/CT images from one representative animal (right panel) bearing (A) 5TGM1 myeloma or (B) MPC-11 myeloma show rapid radioiodine uptake following virus administration. Radioisotope uptake is seen in the thyroid gland (Th), and stomach (St), with slight excreted radioisotope visible in the bladder (Bl). Tumors from control PBS-treated animals (on left) show only background 99m TcO 4 uptake. Semi-quantitative monitoring of intratumoral virus spread in subcutaneous (C) 5TGM1 and (D) MPC-11 tumor models. SPECT/CT images from n=5 VSV-mIFNβ-NIS-treated and n=2 control (PBS-treated) animals were analyzed to quantify 99m TcO 4 radioisotope uptake by tumors days 1 through 5 following virus therapy. Mean group values are plotted for each timepoint (errors bars indicate SEM)

    Article Snippet: BHK-21 and MPC-11 cells, obtained from American Type cell culture (ATCC), were grown in Dulbecco Modified eagles medium (DMEM).

    Techniques: Mouse Assay, Single Photon Emission Computed Tomography, Imaging

    Generation and characterization of VSV expressing IFNβ and NIS (A) Schematic of VSV-IFNβ-NIS. Two viruses were constructed, one encoding mouse IFNβ, the other human IFNβ. (B) One-step virus growth curves on BHK cells infected with VSV-GFP, VSV-mIFNβ-NIS or VSV-hIFNβ-NIS at MOI 1.0; (C) secretion of murine or human IFNβ by VSV-IFNβ-NIS-infected BHK cells, measured by ELISA. n.d. is not detectable. (D) Radioiodine uptakes by BHK cells infected with VSV-GFP, VSV-mIFNβ-NIS or VSV-hIFNβ-NIS at MOI 1.0. Uptakes were determined with (black symbols) or without (grey symbols) potassium perchlorate (KClO 4 ), a specific inhibitor of NIS-mediated radioiodine uptake (E) Killing of myeloma cell lines by VSV. Viability of mouse IFNβ-treated (100U/ml for 12 hours) or untreated 5TGM1 and MPC-11 murine myeloma cells and B-16 murine melanoma cells was assessed by MTT assay at 48h after infection with VSV-GFP (MOI 1.0) and plotted as % viability compared to untreated cells. Significant differences were measured by t-test and P values are shown. (F) Timecourse of 5TGM1 and MPC-11 cell killing was monitored following infection with VSV-mIFNβ-NIS or VSV-hIFNβ-NIS (MOI 1.0) by measuring cell viability at 12h intervals by MTT assay. MPC-11 was killed more rapidly than 5TGM1. Error bars indicate Standard error of the mean (SEM)

    Journal: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K

    Article Title: Curative one-shot systemic virotherapy in murine myeloma

    doi: 10.1038/leu.2012.70

    Figure Lengend Snippet: Generation and characterization of VSV expressing IFNβ and NIS (A) Schematic of VSV-IFNβ-NIS. Two viruses were constructed, one encoding mouse IFNβ, the other human IFNβ. (B) One-step virus growth curves on BHK cells infected with VSV-GFP, VSV-mIFNβ-NIS or VSV-hIFNβ-NIS at MOI 1.0; (C) secretion of murine or human IFNβ by VSV-IFNβ-NIS-infected BHK cells, measured by ELISA. n.d. is not detectable. (D) Radioiodine uptakes by BHK cells infected with VSV-GFP, VSV-mIFNβ-NIS or VSV-hIFNβ-NIS at MOI 1.0. Uptakes were determined with (black symbols) or without (grey symbols) potassium perchlorate (KClO 4 ), a specific inhibitor of NIS-mediated radioiodine uptake (E) Killing of myeloma cell lines by VSV. Viability of mouse IFNβ-treated (100U/ml for 12 hours) or untreated 5TGM1 and MPC-11 murine myeloma cells and B-16 murine melanoma cells was assessed by MTT assay at 48h after infection with VSV-GFP (MOI 1.0) and plotted as % viability compared to untreated cells. Significant differences were measured by t-test and P values are shown. (F) Timecourse of 5TGM1 and MPC-11 cell killing was monitored following infection with VSV-mIFNβ-NIS or VSV-hIFNβ-NIS (MOI 1.0) by measuring cell viability at 12h intervals by MTT assay. MPC-11 was killed more rapidly than 5TGM1. Error bars indicate Standard error of the mean (SEM)

    Article Snippet: BHK-21 and MPC-11 cells, obtained from American Type cell culture (ATCC), were grown in Dulbecco Modified eagles medium (DMEM).

    Techniques: Expressing, Construct, Infection, Enzyme-linked Immunosorbent Assay, MTT Assay

    High-resolution microSPECT/computed tomography (CT) imaging is able to distinguish individual centers of radiotracer uptake. ( a ) Single planes from microSPECT/CT imaging of three different tumor-bearing mice 24 hours after intratumoral vesicular stomatitis virus (VSV)-mIFNβ-NIS infection at doses 5 × 10 6 through 1 × 10 8 TCID 50 . The microSPECT/CT imaging is able to discern individual foci of radiotracer uptake. The distribution and density of the individual foci is dependent on virus dose although variability across mice given the same treatment is seen. ( b ) Serial planes along a single axis through the tumor of an MPC-11 tumor-bearing immunocompetent BALB/c mouse 24 hours after 5 × 10 6 TCID 50 VSV-mIFNβ-NIS shows increasing and decreasing diameter and intensity of a single radiotracer uptake center indicating approximately spherical geometry. Diagram shows the collection and orientation of serial planes.

    Journal: Molecular Therapy Oncolytics

    Article Title: Reporter gene imaging identifies intratumoral infection voids as a critical barrier to systemic oncolytic virus efficacy

    doi: 10.1038/mto.2014.5

    Figure Lengend Snippet: High-resolution microSPECT/computed tomography (CT) imaging is able to distinguish individual centers of radiotracer uptake. ( a ) Single planes from microSPECT/CT imaging of three different tumor-bearing mice 24 hours after intratumoral vesicular stomatitis virus (VSV)-mIFNβ-NIS infection at doses 5 × 10 6 through 1 × 10 8 TCID 50 . The microSPECT/CT imaging is able to discern individual foci of radiotracer uptake. The distribution and density of the individual foci is dependent on virus dose although variability across mice given the same treatment is seen. ( b ) Serial planes along a single axis through the tumor of an MPC-11 tumor-bearing immunocompetent BALB/c mouse 24 hours after 5 × 10 6 TCID 50 VSV-mIFNβ-NIS shows increasing and decreasing diameter and intensity of a single radiotracer uptake center indicating approximately spherical geometry. Diagram shows the collection and orientation of serial planes.

    Article Snippet: MPC-11 murine myeloma cells and BHK-21 cells were obtained from and American Type Culture Collection (Manassas, VA) and cultured in Dulbecco’s modified Eagles medium (DMEM; Mediatech, Herndon, VA) supplemented with 10% FBS.

    Techniques: Computed Tomography, Imaging, Mouse Assay, Infection

    Inhibitory effect of PAb on tumour growth in a mouse model injected subcutaneously with MPC-11. a After mice bearing subcutaneous MPC-11 tumour nodules were injected (i.v.) with PAb, a significant decrease in tumour size was noted that was not seen among the NS-treated and control IgG-treated mice. b Comparison of average tumour humid weights between all groups. *Represents significant difference between the PAb treatment group and both control groups ( P

    Journal: Apoptosis

    Article Title: Polyclonal rabbit anti-murine plasmacytoma cell globulins induce myeloma cells apoptosis and inhibit tumour growth in mice

    doi: 10.1007/s10495-010-0568-7

    Figure Lengend Snippet: Inhibitory effect of PAb on tumour growth in a mouse model injected subcutaneously with MPC-11. a After mice bearing subcutaneous MPC-11 tumour nodules were injected (i.v.) with PAb, a significant decrease in tumour size was noted that was not seen among the NS-treated and control IgG-treated mice. b Comparison of average tumour humid weights between all groups. *Represents significant difference between the PAb treatment group and both control groups ( P

    Article Snippet: In this study, the murine plasmacytoma cell lines MPC-11, SP2/0, and NS-1, along with the murine colon carcinoma cell line CT26 and murine melanoma cell line B16 were obtained from the American type culture collection (ATCC).

    Techniques: Injection, Mouse Assay

    Binding of PAb to murine MPC-11 plasmacytoma cells. a ELISA results of PAb binding to MPC-11 cells. Control rabbit IgG and PAb was incubated with MPC-11 at dilutions of 0.25–2.5 µg/ml. After the addition of alkaline phosphatase conjugated secondary antibody, absorbance was measured at 450 nm. Represented here is the mean of 4–6 wells ± S.D. for every dilution. b Indirect immunofluorescence assay of PAb on myeloma and non-myeloma cell lines by flow cytometry. Grey line represents 1/2000 PAb dilutions reacted with MPC-11 ( left panel ), SP2/0 ( upper part, left panel ) and NS-1 ( upper part, right panel ); murine melanoma cell line B16 ( lower part, left panel ) and murine colon carcinoma cell line CT26 ( lower part, right panel ). Black line represents the control IgG diluted 1/2000 used as negative control. c Indirect immunofluorescence assay of PAb on MPC-11 by fluorescence microscopy with FITC-goat anti-rabbit IgG ( left ; green fluorescence ) and with hoechst33258 ( middle ; blue fluorescence ) . Upper line represents cells treated with PAb, and lower line represents cells treated with control IgG. Merged images ( right ) show localization of PAb on MPC-11 cells (×400) (Color figure online)

    Journal: Apoptosis

    Article Title: Polyclonal rabbit anti-murine plasmacytoma cell globulins induce myeloma cells apoptosis and inhibit tumour growth in mice

    doi: 10.1007/s10495-010-0568-7

    Figure Lengend Snippet: Binding of PAb to murine MPC-11 plasmacytoma cells. a ELISA results of PAb binding to MPC-11 cells. Control rabbit IgG and PAb was incubated with MPC-11 at dilutions of 0.25–2.5 µg/ml. After the addition of alkaline phosphatase conjugated secondary antibody, absorbance was measured at 450 nm. Represented here is the mean of 4–6 wells ± S.D. for every dilution. b Indirect immunofluorescence assay of PAb on myeloma and non-myeloma cell lines by flow cytometry. Grey line represents 1/2000 PAb dilutions reacted with MPC-11 ( left panel ), SP2/0 ( upper part, left panel ) and NS-1 ( upper part, right panel ); murine melanoma cell line B16 ( lower part, left panel ) and murine colon carcinoma cell line CT26 ( lower part, right panel ). Black line represents the control IgG diluted 1/2000 used as negative control. c Indirect immunofluorescence assay of PAb on MPC-11 by fluorescence microscopy with FITC-goat anti-rabbit IgG ( left ; green fluorescence ) and with hoechst33258 ( middle ; blue fluorescence ) . Upper line represents cells treated with PAb, and lower line represents cells treated with control IgG. Merged images ( right ) show localization of PAb on MPC-11 cells (×400) (Color figure online)

    Article Snippet: In this study, the murine plasmacytoma cell lines MPC-11, SP2/0, and NS-1, along with the murine colon carcinoma cell line CT26 and murine melanoma cell line B16 were obtained from the American type culture collection (ATCC).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation, Immunofluorescence, Flow Cytometry, Cytometry, Negative Control, Fluorescence, Microscopy

    The PAb polyclonal antibody was cytotoxic to the myeloma cell MPC-11 line. a MPC-11 cells were cultured with or without PAb in medium containing inactivated FCS at 37°C for 24, 48, or 72 h. Cell viability was measured by a colorimetric assay utilizing MTT. b MPC-11 cells were cultured with or without PAb in medium containing FCS at 37°C for 24, 48, or 72 h and cell viability was measured MTT. c MTT analysis of normal mice spleen cells and purified B and T cells from splenocytes treated with PAb

    Journal: Apoptosis

    Article Title: Polyclonal rabbit anti-murine plasmacytoma cell globulins induce myeloma cells apoptosis and inhibit tumour growth in mice

    doi: 10.1007/s10495-010-0568-7

    Figure Lengend Snippet: The PAb polyclonal antibody was cytotoxic to the myeloma cell MPC-11 line. a MPC-11 cells were cultured with or without PAb in medium containing inactivated FCS at 37°C for 24, 48, or 72 h. Cell viability was measured by a colorimetric assay utilizing MTT. b MPC-11 cells were cultured with or without PAb in medium containing FCS at 37°C for 24, 48, or 72 h and cell viability was measured MTT. c MTT analysis of normal mice spleen cells and purified B and T cells from splenocytes treated with PAb

    Article Snippet: In this study, the murine plasmacytoma cell lines MPC-11, SP2/0, and NS-1, along with the murine colon carcinoma cell line CT26 and murine melanoma cell line B16 were obtained from the American type culture collection (ATCC).

    Techniques: Cell Culture, Colorimetric Assay, MTT Assay, Mouse Assay, Purification

    PAb-induced apoptosis in myeloma cell lines. a–c The MPC-11 was cultured in the presence of NS, control IgG, or PAb (200 µg/ml) in microtiter plates for 48 h before photography. d MPC-11 cells were incubated with PAb (200 µg/ml) in culture medium at 37°C. At 48 h, 10 6 cells were removed and DNA was isolated. M marker, NS normal saline group, C control IgG, S PAb group. e Flow cytometric analysis revealed the proportion of sub-G1 cells (apoptotic cells) to be 7.0% in the NS group ( left panel ), 8.3% in the control IgG group ( middle panel ), and 48.1% in PAb-treated cells ( right panel ). f PAb-induced activity of caspase-3, -8, and -9 in MPC-11 cells. MPC-11 cells were treated with 200 µg/ml PAb ( right lane ), the same concentration of control IgG ( middle lane ), or NS ( left lane ) for 48 h and then lysed as described in “ Materials and methods ”. Protein extracts were immunoblotted to monitor the activation of caspase-3, -8, and -9. β-actin was used as the loading control. g Myeloma cells were pretreated with the caspase inhibitor zVAD-fmk at 100 µM for 1 h before treatment with PAb at 200 µg/ml for 48 h. Cell viability was assessed by MTT assay. *Represents the significant difference in cell viability in presence of zVAD-fmk versus PAb treatment alone. Bar graph indicates the mean ± SD of 3 independent experiments ( P

    Journal: Apoptosis

    Article Title: Polyclonal rabbit anti-murine plasmacytoma cell globulins induce myeloma cells apoptosis and inhibit tumour growth in mice

    doi: 10.1007/s10495-010-0568-7

    Figure Lengend Snippet: PAb-induced apoptosis in myeloma cell lines. a–c The MPC-11 was cultured in the presence of NS, control IgG, or PAb (200 µg/ml) in microtiter plates for 48 h before photography. d MPC-11 cells were incubated with PAb (200 µg/ml) in culture medium at 37°C. At 48 h, 10 6 cells were removed and DNA was isolated. M marker, NS normal saline group, C control IgG, S PAb group. e Flow cytometric analysis revealed the proportion of sub-G1 cells (apoptotic cells) to be 7.0% in the NS group ( left panel ), 8.3% in the control IgG group ( middle panel ), and 48.1% in PAb-treated cells ( right panel ). f PAb-induced activity of caspase-3, -8, and -9 in MPC-11 cells. MPC-11 cells were treated with 200 µg/ml PAb ( right lane ), the same concentration of control IgG ( middle lane ), or NS ( left lane ) for 48 h and then lysed as described in “ Materials and methods ”. Protein extracts were immunoblotted to monitor the activation of caspase-3, -8, and -9. β-actin was used as the loading control. g Myeloma cells were pretreated with the caspase inhibitor zVAD-fmk at 100 µM for 1 h before treatment with PAb at 200 µg/ml for 48 h. Cell viability was assessed by MTT assay. *Represents the significant difference in cell viability in presence of zVAD-fmk versus PAb treatment alone. Bar graph indicates the mean ± SD of 3 independent experiments ( P

    Article Snippet: In this study, the murine plasmacytoma cell lines MPC-11, SP2/0, and NS-1, along with the murine colon carcinoma cell line CT26 and murine melanoma cell line B16 were obtained from the American type culture collection (ATCC).

    Techniques: Cell Culture, Incubation, Isolation, Marker, Flow Cytometry, Activity Assay, Concentration Assay, Activation Assay, MTT Assay

    Proliferation inhibitory effect of deguelin on MPC-11 cells. Concentration- and time-dependent inhibition of the proliferation of MPC-11 cells by deguelin revealed by MTT assay. Cells were seeded in 96-well-plates and treated with various concentrations

    Journal: Oncology Letters

    Article Title: Deguelin, a natural rotenoid, inhibits mouse myeloma cell growth in vitro via induction of apoptosis

    doi: 10.3892/ol.2012.790

    Figure Lengend Snippet: Proliferation inhibitory effect of deguelin on MPC-11 cells. Concentration- and time-dependent inhibition of the proliferation of MPC-11 cells by deguelin revealed by MTT assay. Cells were seeded in 96-well-plates and treated with various concentrations

    Article Snippet: The murine myeloma MPC-11 cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Concentration Assay, Inhibition, MTT Assay

    Morphological changes induced by deguelin. (A and B) Fluorescence and (C and D) bright-field microscope images of PI-stained nuclei of MPC-11 cells following incubation with deguelin for 48 h. (A and C) 0 ng/ml deguelin; (B and D) 100 ng/ml deguelin.

    Journal: Oncology Letters

    Article Title: Deguelin, a natural rotenoid, inhibits mouse myeloma cell growth in vitro via induction of apoptosis

    doi: 10.3892/ol.2012.790

    Figure Lengend Snippet: Morphological changes induced by deguelin. (A and B) Fluorescence and (C and D) bright-field microscope images of PI-stained nuclei of MPC-11 cells following incubation with deguelin for 48 h. (A and C) 0 ng/ml deguelin; (B and D) 100 ng/ml deguelin.

    Article Snippet: The murine myeloma MPC-11 cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Fluorescence, Microscopy, Staining, Incubation

    Patterns of DNA following treatment with deguelin. Following exposure to deguelin for 48 h, agarose gel electrophoresis patterns of DNA isolated from MPC-11 cells treated with (A) 0, (B) 50 and (C) 100 ng/ml deguelin. Ladder-like patterns of DNA fragments

    Journal: Oncology Letters

    Article Title: Deguelin, a natural rotenoid, inhibits mouse myeloma cell growth in vitro via induction of apoptosis

    doi: 10.3892/ol.2012.790

    Figure Lengend Snippet: Patterns of DNA following treatment with deguelin. Following exposure to deguelin for 48 h, agarose gel electrophoresis patterns of DNA isolated from MPC-11 cells treated with (A) 0, (B) 50 and (C) 100 ng/ml deguelin. Ladder-like patterns of DNA fragments

    Article Snippet: The murine myeloma MPC-11 cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Agarose Gel Electrophoresis, Isolation

    Effects of deguelin on Bcl-2 and Bax expression and activation of caspase-3. MPC-11 cells were treated with deguelin at various concentrations (0–100 ng/ml) for 48 h and the expression levels of Bcl-2/Bax and cleaved caspase-3 were analyzed by

    Journal: Oncology Letters

    Article Title: Deguelin, a natural rotenoid, inhibits mouse myeloma cell growth in vitro via induction of apoptosis

    doi: 10.3892/ol.2012.790

    Figure Lengend Snippet: Effects of deguelin on Bcl-2 and Bax expression and activation of caspase-3. MPC-11 cells were treated with deguelin at various concentrations (0–100 ng/ml) for 48 h and the expression levels of Bcl-2/Bax and cleaved caspase-3 were analyzed by

    Article Snippet: The murine myeloma MPC-11 cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing, Activation Assay

    Deguelin inhibits phosphorylation of Akt. MPC-11 cells were incubated with 0–100 ng/ml deguelin for 48 h. Cells were lysed and analyzed by immunoblotting using antibodies specific for Akt. The phosphorylation of Akt was markedly inhibited by deguelin,

    Journal: Oncology Letters

    Article Title: Deguelin, a natural rotenoid, inhibits mouse myeloma cell growth in vitro via induction of apoptosis

    doi: 10.3892/ol.2012.790

    Figure Lengend Snippet: Deguelin inhibits phosphorylation of Akt. MPC-11 cells were incubated with 0–100 ng/ml deguelin for 48 h. Cells were lysed and analyzed by immunoblotting using antibodies specific for Akt. The phosphorylation of Akt was markedly inhibited by deguelin,

    Article Snippet: The murine myeloma MPC-11 cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Incubation

    Phosphorylation of PSF during mitosis and apoptosis. HeLa cells were blocked at G1/S by a thymidine block followed by a nocodazole block causing cells to stop at metaphase. (A) Protein extracts were made from untreated and treated cells, and blots were probed with the B92 antibody. (B) Extracts were made from HeLa cells treated with nocodazole for the indicated time periods. (C) The cells were then released from the mitotic block, and extracts were made after a few hours. (D) Mouse MPC-11 cells underwent the same treatments. (E) Mitotically blocked HeLa protein extracts were treated with λ protein phosphatase at 30°C for 1 h. (F) HL-60 apoptotic cells were separated from nonapoptotic cells with the use of a Percoll gradient. The shift in the apoptotic cells was compared with mitotic cells. All the shifts observed were reproducible.

    Journal: Molecular Biology of the Cell

    Article Title: Nuclear Relocalization of the Pre-mRNA Splicing Factor PSF during Apoptosis Involves Hyperphosphorylation, Masking of Antigenic Epitopes, and Changes in Protein Interactions

    doi:

    Figure Lengend Snippet: Phosphorylation of PSF during mitosis and apoptosis. HeLa cells were blocked at G1/S by a thymidine block followed by a nocodazole block causing cells to stop at metaphase. (A) Protein extracts were made from untreated and treated cells, and blots were probed with the B92 antibody. (B) Extracts were made from HeLa cells treated with nocodazole for the indicated time periods. (C) The cells were then released from the mitotic block, and extracts were made after a few hours. (D) Mouse MPC-11 cells underwent the same treatments. (E) Mitotically blocked HeLa protein extracts were treated with λ protein phosphatase at 30°C for 1 h. (F) HL-60 apoptotic cells were separated from nonapoptotic cells with the use of a Percoll gradient. The shift in the apoptotic cells was compared with mitotic cells. All the shifts observed were reproducible.

    Article Snippet: MPC-11 cells were thymidine (G1/S)-blocked (5 mM, Sigma) for 24 h. The cells were then washed and incubated in medium without thymidine for 2 h before blocking at metaphase by 50 ng/ml nocodazole for 14 h. HeLa cells were thymidine-blocked (2 mM, 8 h) followed by nocodazole block (50 ng/ml, 14 h).

    Techniques: Blocking Assay

    A comparison between the performance of our chip and a Dynal MPC.

    Journal: Biomicrofluidics

    Article Title: Magnetophoretic-based microfluidic device for DNA isolation

    doi: 10.1063/1.4893772

    Figure Lengend Snippet: A comparison between the performance of our chip and a Dynal MPC.

    Article Snippet: Figure shows a comparison between the performance of our chip and a commercially available Dynal MPC.

    Techniques: Chromatin Immunoprecipitation

    Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% MPC-LCFV. Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P

    Journal: Scientific Reports

    Article Title: Novel anti-biofouling light-curable fluoride varnish containing 2-methacryloyloxyethyl phosphorylcholine to prevent enamel demineralization

    doi: 10.1038/s41598-018-38255-2

    Figure Lengend Snippet: Representative live/dead staining images of A. naeslundii ( A ) and S. sanguinis ( C ) cells attached on the surfaces of control and 3% MPC-LCFV. Scale bar is 100 µm. CFU counts derived from A. naeslundii ( B ) and S. sanguinis ( D ) cells attached on the surfaces of control and 3% MPC-LCFV. * P

    Article Snippet: Preparation of MPC-incorporated LCFV Commercially available MPC powder (Sigma-Aldrich, St. Louis, MO, USA) and LCFV (Clinpro XT Varnish; 3 M ESPE, St. Paul, MN, USA) were used in this study.

    Techniques: Staining, Derivative Assay

    Comparison of the optical density (OD) of adsorbed bovine serum albumin (BSA) ( A ) and protein adsorbed form brain heart infusion (BHI) medium ( B ) between LCFV with different concentrations of MPC. Different letters above bars indicate significant differences. *** P

    Journal: Scientific Reports

    Article Title: Novel anti-biofouling light-curable fluoride varnish containing 2-methacryloyloxyethyl phosphorylcholine to prevent enamel demineralization

    doi: 10.1038/s41598-018-38255-2

    Figure Lengend Snippet: Comparison of the optical density (OD) of adsorbed bovine serum albumin (BSA) ( A ) and protein adsorbed form brain heart infusion (BHI) medium ( B ) between LCFV with different concentrations of MPC. Different letters above bars indicate significant differences. *** P

    Article Snippet: Preparation of MPC-incorporated LCFV Commercially available MPC powder (Sigma-Aldrich, St. Louis, MO, USA) and LCFV (Clinpro XT Varnish; 3 M ESPE, St. Paul, MN, USA) were used in this study.

    Techniques:

    Qualitative scanning electron images of S. mutans cells attached to the surfaces of control and experimental groups at a magnification of 5,000× ( A ). Scale bar is 2 µm. Colony-forming unit (CFU) counts derived from S. mutans cells attached on the surfaces of control and MPC-LCFV ( B ). Different letters above bars indicate significant differences. *** P

    Journal: Scientific Reports

    Article Title: Novel anti-biofouling light-curable fluoride varnish containing 2-methacryloyloxyethyl phosphorylcholine to prevent enamel demineralization

    doi: 10.1038/s41598-018-38255-2

    Figure Lengend Snippet: Qualitative scanning electron images of S. mutans cells attached to the surfaces of control and experimental groups at a magnification of 5,000× ( A ). Scale bar is 2 µm. Colony-forming unit (CFU) counts derived from S. mutans cells attached on the surfaces of control and MPC-LCFV ( B ). Different letters above bars indicate significant differences. *** P

    Article Snippet: Preparation of MPC-incorporated LCFV Commercially available MPC powder (Sigma-Aldrich, St. Louis, MO, USA) and LCFV (Clinpro XT Varnish; 3 M ESPE, St. Paul, MN, USA) were used in this study.

    Techniques: Derivative Assay

    Representative photograph of a bovine tooth to which control and 3% MPC-LCFV was applied on each side and that was exposed to BHI culture medium supplemented with 2% sucrose and bacterial inoculum. Trace Disclosing Solution was applied to the sample ( A ). Representative scanning electron image of bacteria attached on the surfaces of the control and 3% MPC-LCFV at a magnification of 100× ( B ). Scale bar is 40 µm. Comparison of enamel surface microhardness loss (%) after exposure to a bacterial culture between the control and 3% MPC-LCFV ( C ). *** P

    Journal: Scientific Reports

    Article Title: Novel anti-biofouling light-curable fluoride varnish containing 2-methacryloyloxyethyl phosphorylcholine to prevent enamel demineralization

    doi: 10.1038/s41598-018-38255-2

    Figure Lengend Snippet: Representative photograph of a bovine tooth to which control and 3% MPC-LCFV was applied on each side and that was exposed to BHI culture medium supplemented with 2% sucrose and bacterial inoculum. Trace Disclosing Solution was applied to the sample ( A ). Representative scanning electron image of bacteria attached on the surfaces of the control and 3% MPC-LCFV at a magnification of 100× ( B ). Scale bar is 40 µm. Comparison of enamel surface microhardness loss (%) after exposure to a bacterial culture between the control and 3% MPC-LCFV ( C ). *** P

    Article Snippet: Preparation of MPC-incorporated LCFV Commercially available MPC powder (Sigma-Aldrich, St. Louis, MO, USA) and LCFV (Clinpro XT Varnish; 3 M ESPE, St. Paul, MN, USA) were used in this study.

    Techniques:

    Representative polarized light microscopy images of a bovine tooth before and 14 days after exposure to BHI culture medium supplemented with 2% sucrose and bacterial inoculum. Specimens were treated with 0% MPC-LCFV (control), 3% MPC-LCFV, or no varnish. Scale bar is 500 µm.

    Journal: Scientific Reports

    Article Title: Novel anti-biofouling light-curable fluoride varnish containing 2-methacryloyloxyethyl phosphorylcholine to prevent enamel demineralization

    doi: 10.1038/s41598-018-38255-2

    Figure Lengend Snippet: Representative polarized light microscopy images of a bovine tooth before and 14 days after exposure to BHI culture medium supplemented with 2% sucrose and bacterial inoculum. Specimens were treated with 0% MPC-LCFV (control), 3% MPC-LCFV, or no varnish. Scale bar is 500 µm.

    Article Snippet: Preparation of MPC-incorporated LCFV Commercially available MPC powder (Sigma-Aldrich, St. Louis, MO, USA) and LCFV (Clinpro XT Varnish; 3 M ESPE, St. Paul, MN, USA) were used in this study.

    Techniques: Light Microscopy