Journal: Journal of leukocyte biology
Article Title: TREM-1 regulates neutrophil chemotaxis by promoting NOX-dependent superoxide production
Figure Lengend Snippet: TREM-1 deficiency impairs neutrophil chemotaxis in a NOX2-dependent manner. Migration using the EZ-TAXIScan device was determined in WT, TREM-1-deficient, CYBB-deficient, and TREM-1/CYBB-deficient neutrophils stimulated with fMLP or ZAS in the presence or absence of 10 μ M DPI. Motility (A), CI (B and C), and instantaneous velocity (D) were measured. Data from 4–12 independent experiments were compiled and shown as Mean ± SEM; each data point represents 3–5 pooled mice. * P
Article Snippet: Genotypes were determined by PCR for both Trem1/3 and Cybb and confirmed by FACs analysis of blood for TREM-1, using anti-mouse TREM-1, clone 174031 (R & D Systems), and dihydrorhodamine (DHR; Thermofisher Scientific) for ROS production with PMA (Sigma) stimulation.
Techniques: Chemotaxis Assay, Migration, Mouse Assay