mouse tbx21 gene Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher gene exp tbx21 mm00450960 m1
    Factor XII deficiency alters T-cell differentiation. ( a ) <t>Tbx21</t> , Gata3 , Rorc and Foxp3 expression from LN cells at day 10 ( d 10 ) or d max as well as from brain-infiltrating leukocytes (BILs) at d max after MOG 35–55 immunization is determined by real-time reverse transcription–PCR using 18S rRNA for normalization. Data (mean±s.e.m. of five experiments) are given as fold change in normalized gene expression in animals relative to WT controls. ( b ) At d 10 after MOG 35–55 immunization, proliferation and cytokine production by CD4 + T cells purified from LN and restimulated with 10 μg ml −1 MOG 35–55 and irradiated (35 Gy) antigen-presenting cells in vitro for 48 h (upper panels), and by CD11c + DCs purified from spleens and incubated with 1 μg ml −1 LPS in vitro for 48 h (lower panels) are shown. ( c ) Mononuclear cells were isolated from the LN of WT and F12 −/− animals at d 10 post induction of EAE. Cells were polyclonal restimulated in vitro , stained with anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry for the percentage of IL-17A-producing CD4 + T cells. ( d ) Cytokine production by purified BILs from MOG 35–55 -immunized WT or F12 −/− mice at d max after restimulation with 10 μg ml −1 MOG 35–55 for 48 h. ( e , f ) BILs and LNs were isolated from WT and F12 −/− animals at d max post induction of EAE and polyclonal restimulated in vitro . For the detection of the percentage of IL-17A-producing lymphocytes (CD45 high CD11b neg cells or CD4 + CD3 + T cells), BILs or LNs were stained with anti-CD11b and anti-CD45, or anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry. In b – f , data are given as means±s.e.m. of three independent experiments, each performed in triplicate. For c , e and f , representative dot plots for IL-17A expression are shown. For a – f , non-parametric Mann–Whitney U -test. * P
    Gene Exp Tbx21 Mm00450960 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp tbx21 mm00450960 m1/product/Thermo Fisher
    Average 99 stars, based on 398 article reviews
    Price from $9.99 to $1999.99
    gene exp tbx21 mm00450960 m1 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    88
    The Jackson Laboratory formal gene name tbx21 mice
    Factor XII deficiency alters T-cell differentiation. ( a ) <t>Tbx21</t> , Gata3 , Rorc and Foxp3 expression from LN cells at day 10 ( d 10 ) or d max as well as from brain-infiltrating leukocytes (BILs) at d max after MOG 35–55 immunization is determined by real-time reverse transcription–PCR using 18S rRNA for normalization. Data (mean±s.e.m. of five experiments) are given as fold change in normalized gene expression in animals relative to WT controls. ( b ) At d 10 after MOG 35–55 immunization, proliferation and cytokine production by CD4 + T cells purified from LN and restimulated with 10 μg ml −1 MOG 35–55 and irradiated (35 Gy) antigen-presenting cells in vitro for 48 h (upper panels), and by CD11c + DCs purified from spleens and incubated with 1 μg ml −1 LPS in vitro for 48 h (lower panels) are shown. ( c ) Mononuclear cells were isolated from the LN of WT and F12 −/− animals at d 10 post induction of EAE. Cells were polyclonal restimulated in vitro , stained with anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry for the percentage of IL-17A-producing CD4 + T cells. ( d ) Cytokine production by purified BILs from MOG 35–55 -immunized WT or F12 −/− mice at d max after restimulation with 10 μg ml −1 MOG 35–55 for 48 h. ( e , f ) BILs and LNs were isolated from WT and F12 −/− animals at d max post induction of EAE and polyclonal restimulated in vitro . For the detection of the percentage of IL-17A-producing lymphocytes (CD45 high CD11b neg cells or CD4 + CD3 + T cells), BILs or LNs were stained with anti-CD11b and anti-CD45, or anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry. In b – f , data are given as means±s.e.m. of three independent experiments, each performed in triplicate. For c , e and f , representative dot plots for IL-17A expression are shown. For a – f , non-parametric Mann–Whitney U -test. * P
    Formal Gene Name Tbx21 Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/formal gene name tbx21 mice/product/The Jackson Laboratory
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    formal gene name tbx21 mice - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    99
    Thermo Fisher gene exp il23r mm00519943 m1
    Factor XII deficiency alters T-cell differentiation. ( a ) <t>Tbx21</t> , Gata3 , Rorc and Foxp3 expression from LN cells at day 10 ( d 10 ) or d max as well as from brain-infiltrating leukocytes (BILs) at d max after MOG 35–55 immunization is determined by real-time reverse transcription–PCR using 18S rRNA for normalization. Data (mean±s.e.m. of five experiments) are given as fold change in normalized gene expression in animals relative to WT controls. ( b ) At d 10 after MOG 35–55 immunization, proliferation and cytokine production by CD4 + T cells purified from LN and restimulated with 10 μg ml −1 MOG 35–55 and irradiated (35 Gy) antigen-presenting cells in vitro for 48 h (upper panels), and by CD11c + DCs purified from spleens and incubated with 1 μg ml −1 LPS in vitro for 48 h (lower panels) are shown. ( c ) Mononuclear cells were isolated from the LN of WT and F12 −/− animals at d 10 post induction of EAE. Cells were polyclonal restimulated in vitro , stained with anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry for the percentage of IL-17A-producing CD4 + T cells. ( d ) Cytokine production by purified BILs from MOG 35–55 -immunized WT or F12 −/− mice at d max after restimulation with 10 μg ml −1 MOG 35–55 for 48 h. ( e , f ) BILs and LNs were isolated from WT and F12 −/− animals at d max post induction of EAE and polyclonal restimulated in vitro . For the detection of the percentage of IL-17A-producing lymphocytes (CD45 high CD11b neg cells or CD4 + CD3 + T cells), BILs or LNs were stained with anti-CD11b and anti-CD45, or anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry. In b – f , data are given as means±s.e.m. of three independent experiments, each performed in triplicate. For c , e and f , representative dot plots for IL-17A expression are shown. For a – f , non-parametric Mann–Whitney U -test. * P
    Gene Exp Il23r Mm00519943 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp il23r mm00519943 m1/product/Thermo Fisher
    Average 99 stars, based on 124 article reviews
    Price from $9.99 to $1999.99
    gene exp il23r mm00519943 m1 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    98
    Thermo Fisher gene exp ahr mm00478932 m1
    Indoleamine 2,3 dioxygenase-1 (IDO1) expression controls the levels of aryl hydrocarbon receptor <t>(AhR)</t> present in CD11b + e CD11c + cells. The number of IDO1 and AhR expressing cells was determined in lung infiltrating leukocytes of wild-type and IDO1 −/− mice 96 h, 2, and 10 weeks after infection with 1 × 10 6 viable Paracoccidioides brasiliensis yeasts. The lung cells were obtained as described in Section “ Materials and Methods ” and labeled with antibodies conjugated to different fluorochromes. The lung infiltrating leukocytes were gated by FSC/SSC analysis. The cells were gated for CD11b + or CD11c + and then for IDO1 and AhR expression. One hundred thousand cells were acquired on FACS CANTO II and subsequently analyzed by FlowJo software. Data are expressed as means ± SEM and are representative of three independent experiments using five mice of each mouse strain per group (** p
    Gene Exp Ahr Mm00478932 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ahr mm00478932 m1/product/Thermo Fisher
    Average 98 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    gene exp ahr mm00478932 m1 - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    Image Search Results


    Factor XII deficiency alters T-cell differentiation. ( a ) Tbx21 , Gata3 , Rorc and Foxp3 expression from LN cells at day 10 ( d 10 ) or d max as well as from brain-infiltrating leukocytes (BILs) at d max after MOG 35–55 immunization is determined by real-time reverse transcription–PCR using 18S rRNA for normalization. Data (mean±s.e.m. of five experiments) are given as fold change in normalized gene expression in animals relative to WT controls. ( b ) At d 10 after MOG 35–55 immunization, proliferation and cytokine production by CD4 + T cells purified from LN and restimulated with 10 μg ml −1 MOG 35–55 and irradiated (35 Gy) antigen-presenting cells in vitro for 48 h (upper panels), and by CD11c + DCs purified from spleens and incubated with 1 μg ml −1 LPS in vitro for 48 h (lower panels) are shown. ( c ) Mononuclear cells were isolated from the LN of WT and F12 −/− animals at d 10 post induction of EAE. Cells were polyclonal restimulated in vitro , stained with anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry for the percentage of IL-17A-producing CD4 + T cells. ( d ) Cytokine production by purified BILs from MOG 35–55 -immunized WT or F12 −/− mice at d max after restimulation with 10 μg ml −1 MOG 35–55 for 48 h. ( e , f ) BILs and LNs were isolated from WT and F12 −/− animals at d max post induction of EAE and polyclonal restimulated in vitro . For the detection of the percentage of IL-17A-producing lymphocytes (CD45 high CD11b neg cells or CD4 + CD3 + T cells), BILs or LNs were stained with anti-CD11b and anti-CD45, or anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry. In b – f , data are given as means±s.e.m. of three independent experiments, each performed in triplicate. For c , e and f , representative dot plots for IL-17A expression are shown. For a – f , non-parametric Mann–Whitney U -test. * P

    Journal: Nature Communications

    Article Title: Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells

    doi: 10.1038/ncomms11626

    Figure Lengend Snippet: Factor XII deficiency alters T-cell differentiation. ( a ) Tbx21 , Gata3 , Rorc and Foxp3 expression from LN cells at day 10 ( d 10 ) or d max as well as from brain-infiltrating leukocytes (BILs) at d max after MOG 35–55 immunization is determined by real-time reverse transcription–PCR using 18S rRNA for normalization. Data (mean±s.e.m. of five experiments) are given as fold change in normalized gene expression in animals relative to WT controls. ( b ) At d 10 after MOG 35–55 immunization, proliferation and cytokine production by CD4 + T cells purified from LN and restimulated with 10 μg ml −1 MOG 35–55 and irradiated (35 Gy) antigen-presenting cells in vitro for 48 h (upper panels), and by CD11c + DCs purified from spleens and incubated with 1 μg ml −1 LPS in vitro for 48 h (lower panels) are shown. ( c ) Mononuclear cells were isolated from the LN of WT and F12 −/− animals at d 10 post induction of EAE. Cells were polyclonal restimulated in vitro , stained with anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry for the percentage of IL-17A-producing CD4 + T cells. ( d ) Cytokine production by purified BILs from MOG 35–55 -immunized WT or F12 −/− mice at d max after restimulation with 10 μg ml −1 MOG 35–55 for 48 h. ( e , f ) BILs and LNs were isolated from WT and F12 −/− animals at d max post induction of EAE and polyclonal restimulated in vitro . For the detection of the percentage of IL-17A-producing lymphocytes (CD45 high CD11b neg cells or CD4 + CD3 + T cells), BILs or LNs were stained with anti-CD11b and anti-CD45, or anti-CD3 and anti-CD4, fixed and permeabilized, stained intracellularly with anti-IL-17A and analysed by flow cytometry. In b – f , data are given as means±s.e.m. of three independent experiments, each performed in triplicate. For c , e and f , representative dot plots for IL-17A expression are shown. For a – f , non-parametric Mann–Whitney U -test. * P

    Article Snippet: rRT–PCR RNA isolation and RT–PCR were performed as previously described following TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA) : Tbx21 (Mm00450960_m1), Gata3 (Mm00484683_m1), Rorc (Mm01261622_m1), Foxp3 (Mm00475162_m1), Brdkb1 (B1R, Mm00432059_s1), Brdkb2 (B2R, Mm00437788_s1), Cd87 (Mm00440911_m1), Par1 (Mm00438851_m1), Par2 (Mm00433160_m1), Par3 (Mm00473929_m1), Par4 (Mm01228147_m1), Il-6 (Mm00446190_m1) and eukaryotic 18S ribosomal RNA (Hs99999901_s1).

    Techniques: Cell Differentiation, Expressing, Polymerase Chain Reaction, Purification, Irradiation, In Vitro, Incubation, Isolation, Staining, Flow Cytometry, Cytometry, Mouse Assay, MANN-WHITNEY

    Indoleamine 2,3 dioxygenase-1 (IDO1) expression controls the levels of aryl hydrocarbon receptor (AhR) present in CD11b + e CD11c + cells. The number of IDO1 and AhR expressing cells was determined in lung infiltrating leukocytes of wild-type and IDO1 −/− mice 96 h, 2, and 10 weeks after infection with 1 × 10 6 viable Paracoccidioides brasiliensis yeasts. The lung cells were obtained as described in Section “ Materials and Methods ” and labeled with antibodies conjugated to different fluorochromes. The lung infiltrating leukocytes were gated by FSC/SSC analysis. The cells were gated for CD11b + or CD11c + and then for IDO1 and AhR expression. One hundred thousand cells were acquired on FACS CANTO II and subsequently analyzed by FlowJo software. Data are expressed as means ± SEM and are representative of three independent experiments using five mice of each mouse strain per group (** p

    Journal: Frontiers in Immunology

    Article Title: The IDO–AhR Axis Controls Th17/Treg Immunity in a Pulmonary Model of Fungal Infection

    doi: 10.3389/fimmu.2017.00880

    Figure Lengend Snippet: Indoleamine 2,3 dioxygenase-1 (IDO1) expression controls the levels of aryl hydrocarbon receptor (AhR) present in CD11b + e CD11c + cells. The number of IDO1 and AhR expressing cells was determined in lung infiltrating leukocytes of wild-type and IDO1 −/− mice 96 h, 2, and 10 weeks after infection with 1 × 10 6 viable Paracoccidioides brasiliensis yeasts. The lung cells were obtained as described in Section “ Materials and Methods ” and labeled with antibodies conjugated to different fluorochromes. The lung infiltrating leukocytes were gated by FSC/SSC analysis. The cells were gated for CD11b + or CD11c + and then for IDO1 and AhR expression. One hundred thousand cells were acquired on FACS CANTO II and subsequently analyzed by FlowJo software. Data are expressed as means ± SEM and are representative of three independent experiments using five mice of each mouse strain per group (** p

    Article Snippet: Real-time Quantitative Polymerase Chain Reaction (RT-PCR) The cDNA was amplified using TaqMan Universal PCR Master Mix (Applied Biosystems) and pre-developed TaqMan assay primers and probes (Ahr , Mm00478932_m1, Ifng , Mm001168134_m1, Tnf , Mm99999068_m1, Il-6 , Mm00446190_m1, Il-10 , Mm00439614_m1, Tgfb1 , Mm00117882_m1, Il-17 , Mm00439618_m1, Il-22 , Mm01226722_m1, Tbx21 , Mm00450960_m1; GATA3 , Mm00484683_m1; Rorc , Mm01261022_m1; Foxp3 , Mm00475162_m1; all from Applied Biosystems).

    Techniques: Expressing, Mouse Assay, Infection, Labeling, FACS, Software