mouse tail dna Search Results


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  • 99
    Millipore mouse tail dna
    TLR4 genotyping. A 415 bp <t>DNA</t> fragment was amplified by <t>PCR</t> using mouse tail DNA and two TLR4 -specific primers, TLR4sen193 and TLR4ant607. The mutation in the TLR4 gene in C3H/HeJ mice creates an Nla III site in the 415 bp DNA fragment. Nla III digestion of the amplified DNA fragment from a mouse with the mutation produces a 304 and 111 bp DNA fragments. The restriction enzyme polymorphism is shown in the picture for each TLR4 genotype.
    Mouse Tail Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    ATUM mouse tail dna
    GCS transgene integration and expression. (A) Xba I/Bgl II restriction mapping of the mouse Ugcg (GCS) gene. Ugcg gene spans 33.5 kb of length and contains 9 exons (solid blocks). The scale bar is 2 kb. (B) The mouse GCS cDNA transgene was cloned into the Hind III/Xba I sites of pBROAD vector containing the ubiquitously expressed ROSA26 promoter and β-globin poly(A) sequence. Arrows show the position of 0.6 kb cDNA probe (exons 6 - 9) for Southern blotting. (C) Fluorescence in situ hybridization (FISH) study. Fibroblasts from WT ( Ugcg+/+ ) (upper panels) and <t>GCStg</t> mice (lower panels) were processed for FISH analysis using both genomic BAC probe RP23 (green, for endogenous Ugcg loci) and GCS cDNA probe (red, for GCStg) as indicated. (D) Southern blot analysis. 20 µg of tail <t>DNA</t> from GCS transgene positive (+) or negative (−) mice were digested by restriction enzymes Xba I and Bgl II and processed for Southern blotting using 0.6 kb [ 32 P]-GCS cDNA probe (shown in B). Lanes 1 to 5 from GCStg lines, Lane 1, no Xba I/Bgl II digestion; lane 2 to 5 (GCStg line 1, 3, 9, 10), with Xba I/Bgl II digestion; lane 6 and 7, 1 x or 2 x copy number of 1.2 kb GCS cDNA fragment loaded on the gel. Images of 1.2 kb GCS cDNA bands on Southern blot were quantitated using Image J 1.47V software (NIH, USA). (E) RT-PCR analysis of GCS transgene expression. RNAs from mouse tissues of 2 transgenic mouse lines (GCStg1 and GCStg3) were conducted for RT-PCR using GCStg specific primers and endogenous Ugcg primers, respectively. Br, brain; Lv, liver; Lu, lung and Sp, spleen.
    Mouse Tail Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega mouse tail dna purification kit
    GCS transgene integration and expression. (A) Xba I/Bgl II restriction mapping of the mouse Ugcg (GCS) gene. Ugcg gene spans 33.5 kb of length and contains 9 exons (solid blocks). The scale bar is 2 kb. (B) The mouse GCS cDNA transgene was cloned into the Hind III/Xba I sites of pBROAD vector containing the ubiquitously expressed ROSA26 promoter and β-globin poly(A) sequence. Arrows show the position of 0.6 kb cDNA probe (exons 6 - 9) for Southern blotting. (C) Fluorescence in situ hybridization (FISH) study. Fibroblasts from WT ( Ugcg+/+ ) (upper panels) and <t>GCStg</t> mice (lower panels) were processed for FISH analysis using both genomic BAC probe RP23 (green, for endogenous Ugcg loci) and GCS cDNA probe (red, for GCStg) as indicated. (D) Southern blot analysis. 20 µg of tail <t>DNA</t> from GCS transgene positive (+) or negative (−) mice were digested by restriction enzymes Xba I and Bgl II and processed for Southern blotting using 0.6 kb [ 32 P]-GCS cDNA probe (shown in B). Lanes 1 to 5 from GCStg lines, Lane 1, no Xba I/Bgl II digestion; lane 2 to 5 (GCStg line 1, 3, 9, 10), with Xba I/Bgl II digestion; lane 6 and 7, 1 x or 2 x copy number of 1.2 kb GCS cDNA fragment loaded on the gel. Images of 1.2 kb GCS cDNA bands on Southern blot were quantitated using Image J 1.47V software (NIH, USA). (E) RT-PCR analysis of GCS transgene expression. RNAs from mouse tissues of 2 transgenic mouse lines (GCStg1 and GCStg3) were conducted for RT-PCR using GCStg specific primers and endogenous Ugcg primers, respectively. Br, brain; Lv, liver; Lu, lung and Sp, spleen.
    Mouse Tail Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    The Jackson Laboratory mouse tail dna
    GCS transgene integration and expression. (A) Xba I/Bgl II restriction mapping of the mouse Ugcg (GCS) gene. Ugcg gene spans 33.5 kb of length and contains 9 exons (solid blocks). The scale bar is 2 kb. (B) The mouse GCS cDNA transgene was cloned into the Hind III/Xba I sites of pBROAD vector containing the ubiquitously expressed ROSA26 promoter and β-globin poly(A) sequence. Arrows show the position of 0.6 kb cDNA probe (exons 6 - 9) for Southern blotting. (C) Fluorescence in situ hybridization (FISH) study. Fibroblasts from WT ( Ugcg+/+ ) (upper panels) and <t>GCStg</t> mice (lower panels) were processed for FISH analysis using both genomic BAC probe RP23 (green, for endogenous Ugcg loci) and GCS cDNA probe (red, for GCStg) as indicated. (D) Southern blot analysis. 20 µg of tail <t>DNA</t> from GCS transgene positive (+) or negative (−) mice were digested by restriction enzymes Xba I and Bgl II and processed for Southern blotting using 0.6 kb [ 32 P]-GCS cDNA probe (shown in B). Lanes 1 to 5 from GCStg lines, Lane 1, no Xba I/Bgl II digestion; lane 2 to 5 (GCStg line 1, 3, 9, 10), with Xba I/Bgl II digestion; lane 6 and 7, 1 x or 2 x copy number of 1.2 kb GCS cDNA fragment loaded on the gel. Images of 1.2 kb GCS cDNA bands on Southern blot were quantitated using Image J 1.47V software (NIH, USA). (E) RT-PCR analysis of GCS transgene expression. RNAs from mouse tissues of 2 transgenic mouse lines (GCStg1 and GCStg3) were conducted for RT-PCR using GCStg specific primers and endogenous Ugcg primers, respectively. Br, brain; Lv, liver; Lu, lung and Sp, spleen.
    Mouse Tail Dna, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega maxwell 16 mouse tail dna purification kit
    GCS transgene integration and expression. (A) Xba I/Bgl II restriction mapping of the mouse Ugcg (GCS) gene. Ugcg gene spans 33.5 kb of length and contains 9 exons (solid blocks). The scale bar is 2 kb. (B) The mouse GCS cDNA transgene was cloned into the Hind III/Xba I sites of pBROAD vector containing the ubiquitously expressed ROSA26 promoter and β-globin poly(A) sequence. Arrows show the position of 0.6 kb cDNA probe (exons 6 - 9) for Southern blotting. (C) Fluorescence in situ hybridization (FISH) study. Fibroblasts from WT ( Ugcg+/+ ) (upper panels) and <t>GCStg</t> mice (lower panels) were processed for FISH analysis using both genomic BAC probe RP23 (green, for endogenous Ugcg loci) and GCS cDNA probe (red, for GCStg) as indicated. (D) Southern blot analysis. 20 µg of tail <t>DNA</t> from GCS transgene positive (+) or negative (−) mice were digested by restriction enzymes Xba I and Bgl II and processed for Southern blotting using 0.6 kb [ 32 P]-GCS cDNA probe (shown in B). Lanes 1 to 5 from GCStg lines, Lane 1, no Xba I/Bgl II digestion; lane 2 to 5 (GCStg line 1, 3, 9, 10), with Xba I/Bgl II digestion; lane 6 and 7, 1 x or 2 x copy number of 1.2 kb GCS cDNA fragment loaded on the gel. Images of 1.2 kb GCS cDNA bands on Southern blot were quantitated using Image J 1.47V software (NIH, USA). (E) RT-PCR analysis of GCS transgene expression. RNAs from mouse tissues of 2 transgenic mouse lines (GCStg1 and GCStg3) were conducted for RT-PCR using GCStg specific primers and endogenous Ugcg primers, respectively. Br, brain; Lv, liver; Lu, lung and Sp, spleen.
    Maxwell 16 Mouse Tail Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    EZ Bioresearch ezhmw mouse tail dna isolation kit
    GCS transgene integration and expression. (A) Xba I/Bgl II restriction mapping of the mouse Ugcg (GCS) gene. Ugcg gene spans 33.5 kb of length and contains 9 exons (solid blocks). The scale bar is 2 kb. (B) The mouse GCS cDNA transgene was cloned into the Hind III/Xba I sites of pBROAD vector containing the ubiquitously expressed ROSA26 promoter and β-globin poly(A) sequence. Arrows show the position of 0.6 kb cDNA probe (exons 6 - 9) for Southern blotting. (C) Fluorescence in situ hybridization (FISH) study. Fibroblasts from WT ( Ugcg+/+ ) (upper panels) and <t>GCStg</t> mice (lower panels) were processed for FISH analysis using both genomic BAC probe RP23 (green, for endogenous Ugcg loci) and GCS cDNA probe (red, for GCStg) as indicated. (D) Southern blot analysis. 20 µg of tail <t>DNA</t> from GCS transgene positive (+) or negative (−) mice were digested by restriction enzymes Xba I and Bgl II and processed for Southern blotting using 0.6 kb [ 32 P]-GCS cDNA probe (shown in B). Lanes 1 to 5 from GCStg lines, Lane 1, no Xba I/Bgl II digestion; lane 2 to 5 (GCStg line 1, 3, 9, 10), with Xba I/Bgl II digestion; lane 6 and 7, 1 x or 2 x copy number of 1.2 kb GCS cDNA fragment loaded on the gel. Images of 1.2 kb GCS cDNA bands on Southern blot were quantitated using Image J 1.47V software (NIH, USA). (E) RT-PCR analysis of GCS transgene expression. RNAs from mouse tissues of 2 transgenic mouse lines (GCStg1 and GCStg3) were conducted for RT-PCR using GCStg specific primers and endogenous Ugcg primers, respectively. Br, brain; Lv, liver; Lu, lung and Sp, spleen.
    Ezhmw Mouse Tail Dna Isolation Kit, supplied by EZ Bioresearch, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Charles River Laboratories mouse tail dna
    GCS transgene integration and expression. (A) Xba I/Bgl II restriction mapping of the mouse Ugcg (GCS) gene. Ugcg gene spans 33.5 kb of length and contains 9 exons (solid blocks). The scale bar is 2 kb. (B) The mouse GCS cDNA transgene was cloned into the Hind III/Xba I sites of pBROAD vector containing the ubiquitously expressed ROSA26 promoter and β-globin poly(A) sequence. Arrows show the position of 0.6 kb cDNA probe (exons 6 - 9) for Southern blotting. (C) Fluorescence in situ hybridization (FISH) study. Fibroblasts from WT ( Ugcg+/+ ) (upper panels) and <t>GCStg</t> mice (lower panels) were processed for FISH analysis using both genomic BAC probe RP23 (green, for endogenous Ugcg loci) and GCS cDNA probe (red, for GCStg) as indicated. (D) Southern blot analysis. 20 µg of tail <t>DNA</t> from GCS transgene positive (+) or negative (−) mice were digested by restriction enzymes Xba I and Bgl II and processed for Southern blotting using 0.6 kb [ 32 P]-GCS cDNA probe (shown in B). Lanes 1 to 5 from GCStg lines, Lane 1, no Xba I/Bgl II digestion; lane 2 to 5 (GCStg line 1, 3, 9, 10), with Xba I/Bgl II digestion; lane 6 and 7, 1 x or 2 x copy number of 1.2 kb GCS cDNA fragment loaded on the gel. Images of 1.2 kb GCS cDNA bands on Southern blot were quantitated using Image J 1.47V software (NIH, USA). (E) RT-PCR analysis of GCS transgene expression. RNAs from mouse tissues of 2 transgenic mouse lines (GCStg1 and GCStg3) were conducted for RT-PCR using GCStg specific primers and endogenous Ugcg primers, respectively. Br, brain; Lv, liver; Lu, lung and Sp, spleen.
    Mouse Tail Dna, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Gen-Probe mouse tail genomic dna kit
    GCS transgene integration and expression. (A) Xba I/Bgl II restriction mapping of the mouse Ugcg (GCS) gene. Ugcg gene spans 33.5 kb of length and contains 9 exons (solid blocks). The scale bar is 2 kb. (B) The mouse GCS cDNA transgene was cloned into the Hind III/Xba I sites of pBROAD vector containing the ubiquitously expressed ROSA26 promoter and β-globin poly(A) sequence. Arrows show the position of 0.6 kb cDNA probe (exons 6 - 9) for Southern blotting. (C) Fluorescence in situ hybridization (FISH) study. Fibroblasts from WT ( Ugcg+/+ ) (upper panels) and <t>GCStg</t> mice (lower panels) were processed for FISH analysis using both genomic BAC probe RP23 (green, for endogenous Ugcg loci) and GCS cDNA probe (red, for GCStg) as indicated. (D) Southern blot analysis. 20 µg of tail <t>DNA</t> from GCS transgene positive (+) or negative (−) mice were digested by restriction enzymes Xba I and Bgl II and processed for Southern blotting using 0.6 kb [ 32 P]-GCS cDNA probe (shown in B). Lanes 1 to 5 from GCStg lines, Lane 1, no Xba I/Bgl II digestion; lane 2 to 5 (GCStg line 1, 3, 9, 10), with Xba I/Bgl II digestion; lane 6 and 7, 1 x or 2 x copy number of 1.2 kb GCS cDNA fragment loaded on the gel. Images of 1.2 kb GCS cDNA bands on Southern blot were quantitated using Image J 1.47V software (NIH, USA). (E) RT-PCR analysis of GCS transgene expression. RNAs from mouse tissues of 2 transgenic mouse lines (GCStg1 and GCStg3) were conducted for RT-PCR using GCStg specific primers and endogenous Ugcg primers, respectively. Br, brain; Lv, liver; Lu, lung and Sp, spleen.
    Mouse Tail Genomic Dna Kit, supplied by Gen-Probe, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    TaKaRa mouse tail genomic dna
    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
    Mouse Tail Genomic Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Qiagen puregene dna kit protocol
    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
    Puregene Dna Kit Protocol, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega mouse tail genomic dna
    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
    Mouse Tail Genomic Dna, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    5 PRIME archivepure dna mouse tail kit
    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
    Archivepure Dna Mouse Tail Kit, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Qiagen mouse tail genomic dna
    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
    Mouse Tail Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega maxwell 16 mouse tail dna purification kit cartridges
    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
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    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
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    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
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    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
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    Nrf2 transcriptional regulation of <t>Gbe1</t> and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). <t>Nrf2-DNA</t> complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P
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    Image Search Results


    TLR4 genotyping. A 415 bp DNA fragment was amplified by PCR using mouse tail DNA and two TLR4 -specific primers, TLR4sen193 and TLR4ant607. The mutation in the TLR4 gene in C3H/HeJ mice creates an Nla III site in the 415 bp DNA fragment. Nla III digestion of the amplified DNA fragment from a mouse with the mutation produces a 304 and 111 bp DNA fragments. The restriction enzyme polymorphism is shown in the picture for each TLR4 genotype.

    Journal: Brain : a journal of neurology

    Article Title: Role of toll-like receptor signalling in Aβ uptake and clearance

    doi: 10.1093/brain/awl249

    Figure Lengend Snippet: TLR4 genotyping. A 415 bp DNA fragment was amplified by PCR using mouse tail DNA and two TLR4 -specific primers, TLR4sen193 and TLR4ant607. The mutation in the TLR4 gene in C3H/HeJ mice creates an Nla III site in the 415 bp DNA fragment. Nla III digestion of the amplified DNA fragment from a mouse with the mutation produces a 304 and 111 bp DNA fragments. The restriction enzyme polymorphism is shown in the picture for each TLR4 genotype.

    Article Snippet: Mouse tail DNA was extracted using the Extract-N-Amp tissue PCR kit (Sigma-Aldrich, St Louis, MO) according to the manufacturer’s protocol.

    Techniques: Amplification, Polymerase Chain Reaction, Mutagenesis, Mouse Assay

    GCS transgene integration and expression. (A) Xba I/Bgl II restriction mapping of the mouse Ugcg (GCS) gene. Ugcg gene spans 33.5 kb of length and contains 9 exons (solid blocks). The scale bar is 2 kb. (B) The mouse GCS cDNA transgene was cloned into the Hind III/Xba I sites of pBROAD vector containing the ubiquitously expressed ROSA26 promoter and β-globin poly(A) sequence. Arrows show the position of 0.6 kb cDNA probe (exons 6 - 9) for Southern blotting. (C) Fluorescence in situ hybridization (FISH) study. Fibroblasts from WT ( Ugcg+/+ ) (upper panels) and GCStg mice (lower panels) were processed for FISH analysis using both genomic BAC probe RP23 (green, for endogenous Ugcg loci) and GCS cDNA probe (red, for GCStg) as indicated. (D) Southern blot analysis. 20 µg of tail DNA from GCS transgene positive (+) or negative (−) mice were digested by restriction enzymes Xba I and Bgl II and processed for Southern blotting using 0.6 kb [ 32 P]-GCS cDNA probe (shown in B). Lanes 1 to 5 from GCStg lines, Lane 1, no Xba I/Bgl II digestion; lane 2 to 5 (GCStg line 1, 3, 9, 10), with Xba I/Bgl II digestion; lane 6 and 7, 1 x or 2 x copy number of 1.2 kb GCS cDNA fragment loaded on the gel. Images of 1.2 kb GCS cDNA bands on Southern blot were quantitated using Image J 1.47V software (NIH, USA). (E) RT-PCR analysis of GCS transgene expression. RNAs from mouse tissues of 2 transgenic mouse lines (GCStg1 and GCStg3) were conducted for RT-PCR using GCStg specific primers and endogenous Ugcg primers, respectively. Br, brain; Lv, liver; Lu, lung and Sp, spleen.

    Journal: PLoS ONE

    Article Title: Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model

    doi: 10.1371/journal.pone.0116023

    Figure Lengend Snippet: GCS transgene integration and expression. (A) Xba I/Bgl II restriction mapping of the mouse Ugcg (GCS) gene. Ugcg gene spans 33.5 kb of length and contains 9 exons (solid blocks). The scale bar is 2 kb. (B) The mouse GCS cDNA transgene was cloned into the Hind III/Xba I sites of pBROAD vector containing the ubiquitously expressed ROSA26 promoter and β-globin poly(A) sequence. Arrows show the position of 0.6 kb cDNA probe (exons 6 - 9) for Southern blotting. (C) Fluorescence in situ hybridization (FISH) study. Fibroblasts from WT ( Ugcg+/+ ) (upper panels) and GCStg mice (lower panels) were processed for FISH analysis using both genomic BAC probe RP23 (green, for endogenous Ugcg loci) and GCS cDNA probe (red, for GCStg) as indicated. (D) Southern blot analysis. 20 µg of tail DNA from GCS transgene positive (+) or negative (−) mice were digested by restriction enzymes Xba I and Bgl II and processed for Southern blotting using 0.6 kb [ 32 P]-GCS cDNA probe (shown in B). Lanes 1 to 5 from GCStg lines, Lane 1, no Xba I/Bgl II digestion; lane 2 to 5 (GCStg line 1, 3, 9, 10), with Xba I/Bgl II digestion; lane 6 and 7, 1 x or 2 x copy number of 1.2 kb GCS cDNA fragment loaded on the gel. Images of 1.2 kb GCS cDNA bands on Southern blot were quantitated using Image J 1.47V software (NIH, USA). (E) RT-PCR analysis of GCS transgene expression. RNAs from mouse tissues of 2 transgenic mouse lines (GCStg1 and GCStg3) were conducted for RT-PCR using GCStg specific primers and endogenous Ugcg primers, respectively. Br, brain; Lv, liver; Lu, lung and Sp, spleen.

    Article Snippet: Briefly, mouse tail DNA (20 µg) from GCStg PCR-positive lines was digested using Hind III and Xba I, separated on 0.8% agarose gels, and transferred to Hybond N+ nylon membranes.

    Techniques: Expressing, Clone Assay, Plasmid Preparation, Sequencing, Southern Blot, Fluorescence, In Situ Hybridization, Fluorescence In Situ Hybridization, Mouse Assay, BAC Assay, Software, Reverse Transcription Polymerase Chain Reaction, Transgenic Assay

    Nrf2 transcriptional regulation of Gbe1 and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). Nrf2-DNA complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P

    Journal: Molecular and Cellular Biology

    Article Title: Nrf2-Mediated Regulation of Skeletal Muscle Glycogen Metabolism

    doi: 10.1128/MCB.01095-15

    Figure Lengend Snippet: Nrf2 transcriptional regulation of Gbe1 and Phka1 expression. (A) Screen shots of ChIP-seq profiles around the Gbe1 and Nqo1 TSSs from two independent experiments (Exp.) with CDDO-Im (100 nmol/liter, 3 h)-treated C2C12 myotubes and anti-Nrf2 antibody. (B) Manual ChIP analysis of Gbe1 (kb −0.4 and kb +15 from the TSS) and Nqo1 (a positive locus). Nrf2-DNA complexes were immunoprecipitated with either anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3) from nuclear extracts of C2C12 myotubes cultured with either 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. The data are percentages of the input DNA. (C) Immunoblot analysis of Nrf2 and lamin B from nuclear extracts of C2C12 myotubes cultured in 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h. (D) Schematic of the chimeric reporter containing the 5′-flanking region of the mouse Gbe1 gene and luciferase cDNA. SV40, simian virus 40. (E and F) Luciferase reporter analysis in C2C12 myoblasts. C2C12 myoblasts were transiently transfected with reporter vectors and cultured for 24 h with the concentrations of CDDO-Im indicated. Analysis of luciferase reporter expression in response to various doses of CDDO-Im (E) and mutational analysis of Gbe1 AREs (F). The transcriptional activities at dose 0 (E, 0.1% DMSO vehicle) and of the vehicle-treated wild-type reporter (F) were set as 1 ( n = 4). Data are relative firefly luciferase activity (test) normalized to Renilla luciferase activity (control). (G) Manual ChIP analysis of Phka1 (kb −1.6 from the TSS) in nuclear extracts from C2C12 myotubes cultured with 0.1% DMSO or 100 nmol/liter CDDO-Im for 3 h with anti-Nrf2 antibody ( n = 4) or control IgG ( n = 3). The data are the percentages of the input DNA. Error bars show the mean ± SEM. ***, P

    Article Snippet: Mouse Gbe1 genomic DNA was PCR amplified from mouse tail genomic DNA with PrimeSTAR (TaKaRa).

    Techniques: Expressing, Chromatin Immunoprecipitation, Immunoprecipitation, Cell Culture, Luciferase, Transfection, Activity Assay