Journal: Acta Pharmacologica Sinica

Article Title: A new recombinant MS-superoxide dismutase alleviates 5-fluorouracil-induced intestinal mucositis in mice

doi: 10.1038/s41401-019-0295-8

Figure Lengend Snippet: Effects of MS-SOD on tight junctions and cytokines in the small intestine after 5-FU treatment. qPCR analysis was performed to measure the transcription levels of occludin ( a ), IL-6, IL-17α, IL-22 ( c ), CXCL1 and CXCL2 ( d ). Western blotting was used to measure the translation level of occludin ( a ). An ELISA was used to measure the IL-6 concentration in serum ( c ). Both occludin mRNA and protein levels in the small intestine were decreased in mice after 5 days of 5-FU injection but were increased in the MS-SOD group. The mRNA expression levels of the main pro-inflammatory cytokines, IL-6, IL-17α and IL-22 as well as the chemokines CXCL1 ans CXCL2, were upregulated in the 5-FU group, but these cytokines were downregulated in MS-SOD-treated mice. The serum IL-6 concentration was in accordance with the mRNA level. ELISA method was used for MPO content measure. MPO content in intestinal tissue homogenates was significantly increased in the 5-FU group, but did not decrease in MS-SOD group ( b ). The data are shown as the means ± SEM ( n = 6). * P < 0.05, ** P < 0.01, *** P < 0.001. In a , the repeated blots in each group indicate three individuals

Article Snippet: Moreover, a mouse myeloperoxidase (MPO) ELISA kit (Cusabio, Wuhan, China) was used to detect MPO content.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Concentration Assay, Injection, Expressing