mouse monoclonal antibody Search Results


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  • 86
    Thermo Fisher mouse antihuman monoclonal antibodies mabs
    <t>CD209</t> + (DC-sign) cells and CD68 + , CD163 + monocyte/macrophage cell markers colocalize on the same cell subsets in normal sigmoid colon and rectum. Normal human sigmoid colon and rectum were labeled with <t>mAbs</t> for CD209, CD68, and CD163 and visualized using
    Mouse Antihuman Monoclonal Antibodies Mabs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mouse mabs
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Mabs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam mouse monoclonal moab antibody
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Moab Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse monoclonal antibody mab
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Covance mouse monoclonal antibody mab
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab, supplied by Covance, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Active Motif mouse monoclonal antibody mab
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab, supplied by Active Motif, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim mouse monoclonal antibody mab
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Oncogene Science Inc mouse monoclonal antibody mab pc10
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab Pc10, supplied by Oncogene Science Inc, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    VMRD Inc mouse monoclonal antibody mab dh59b
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab Dh59b, supplied by VMRD Inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa mouse monoclonal antibody mab
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc mouse monoclonal antibody mab
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology 211 mouse monoclonal antibody mab
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    211 Mouse Monoclonal Antibody Mab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse monoclonal antibody mab p4d1
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab P4d1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mouse monoclonal antibody mab v5
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab V5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Upstate Biotechnology Inc mouse monoclonal antibody mab 4g10
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab 4g10, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mouse monoclonal antibody mab
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad mouse monoclonal antibody mab
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Covance mouse monoclonal antibody mab 16b12
    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for <t>HCMV</t> IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL <t>mAb</t> 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p
    Mouse Monoclonal Antibody Mab 16b12, supplied by Covance, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad mouse monoclonal antibody mab 2g11
    Immunohistochemistry representative of ovine L-BSE. All figures illustrate labelling with mAb <t>2G11,</t> except B . Neuronal and particulate labelling is present in the DNV with mAb 2G11 ( A ), but absent with mAb P4 ( B ) (case 455/11). Particulate labelling and small aggregates are abundant in many areas, such as the thalamic nuclei ( C ) (case 1591/10). Perineuronal labelling in the putamen is a consistent and striking feature of ovine L-BSE ( D ) (case 1591/10), as is intracellular labelling of oligodendrocytes, seen here in the spinocerebellar tract (rostral medulla) ( E ) (case 58/11). Intraneuronal labelling is also present in the DRG ( F ) (case 4/12). Heavy labelling in muscle spindles is also visible ( G ) (case 267/11) and also in occasional myocytes ( H ) (case 98/11). Labelling was also present in the LRS ( I ) and ENS (J) of one VRQ/VRQ recipient (case 48/13).
    Mouse Monoclonal Antibody Mab 2g11, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Millipore mouse monoclonal antibody 355
    Immunohistochemistry representative of ovine L-BSE. All figures illustrate labelling with mAb <t>2G11,</t> except B . Neuronal and particulate labelling is present in the DNV with mAb 2G11 ( A ), but absent with mAb P4 ( B ) (case 455/11). Particulate labelling and small aggregates are abundant in many areas, such as the thalamic nuclei ( C ) (case 1591/10). Perineuronal labelling in the putamen is a consistent and striking feature of ovine L-BSE ( D ) (case 1591/10), as is intracellular labelling of oligodendrocytes, seen here in the spinocerebellar tract (rostral medulla) ( E ) (case 58/11). Intraneuronal labelling is also present in the DRG ( F ) (case 4/12). Heavy labelling in muscle spindles is also visible ( G ) (case 267/11) and also in occasional myocytes ( H ) (case 98/11). Labelling was also present in the LRS ( I ) and ENS (J) of one VRQ/VRQ recipient (case 48/13).
    Mouse Monoclonal Antibody 355, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA mouse monoclonal antibody mab 1922
    Immunohistochemistry representative of ovine L-BSE. All figures illustrate labelling with mAb <t>2G11,</t> except B . Neuronal and particulate labelling is present in the DNV with mAb 2G11 ( A ), but absent with mAb P4 ( B ) (case 455/11). Particulate labelling and small aggregates are abundant in many areas, such as the thalamic nuclei ( C ) (case 1591/10). Perineuronal labelling in the putamen is a consistent and striking feature of ovine L-BSE ( D ) (case 1591/10), as is intracellular labelling of oligodendrocytes, seen here in the spinocerebellar tract (rostral medulla) ( E ) (case 58/11). Intraneuronal labelling is also present in the DRG ( F ) (case 4/12). Heavy labelling in muscle spindles is also visible ( G ) (case 267/11) and also in occasional myocytes ( H ) (case 98/11). Labelling was also present in the LRS ( I ) and ENS (J) of one VRQ/VRQ recipient (case 48/13).
    Mouse Monoclonal Antibody Mab 1922, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CD209 + (DC-sign) cells and CD68 + , CD163 + monocyte/macrophage cell markers colocalize on the same cell subsets in normal sigmoid colon and rectum. Normal human sigmoid colon and rectum were labeled with mAbs for CD209, CD68, and CD163 and visualized using

    Journal: AIDS Research and Human Retroviruses

    Article Title: Antigen-Presenting Cell Candidates for HIV-1 Transmission in Human Distal Colonic Mucosa Defined by CD207 Dendritic Cells and CD209 Macrophages

    doi: 10.1089/aid.2013.0145

    Figure Lengend Snippet: CD209 + (DC-sign) cells and CD68 + , CD163 + monocyte/macrophage cell markers colocalize on the same cell subsets in normal sigmoid colon and rectum. Normal human sigmoid colon and rectum were labeled with mAbs for CD209, CD68, and CD163 and visualized using

    Article Snippet: Indirect immunofluorescence was performed by serially incubating cryostat-prepared tissue sections with mouse antihuman monoclonal antibodies (mAbs) to CD1a, CD207, CD209, CD68, and CD163 of different isotypes followed by incubation with isotype-specific fluorochrome- (Alexa 488 and Alexa 568, Molecular Probes, Eugene, OR) labeled goat antimouse Ig antibodies.

    Techniques: Labeling

    CD209 + (DC-SIGN) cells are expressed on different cells subsets than DC markers. Normal human rectum was labeled with mAbs for CD209, CD207, CD1a, CD1b, and CD1c and then visualized using confocal microscopy. CD209 + (red images) did not colocolize with

    Journal: AIDS Research and Human Retroviruses

    Article Title: Antigen-Presenting Cell Candidates for HIV-1 Transmission in Human Distal Colonic Mucosa Defined by CD207 Dendritic Cells and CD209 Macrophages

    doi: 10.1089/aid.2013.0145

    Figure Lengend Snippet: CD209 + (DC-SIGN) cells are expressed on different cells subsets than DC markers. Normal human rectum was labeled with mAbs for CD209, CD207, CD1a, CD1b, and CD1c and then visualized using confocal microscopy. CD209 + (red images) did not colocolize with

    Article Snippet: Indirect immunofluorescence was performed by serially incubating cryostat-prepared tissue sections with mouse antihuman monoclonal antibodies (mAbs) to CD1a, CD207, CD209, CD68, and CD163 of different isotypes followed by incubation with isotype-specific fluorochrome- (Alexa 488 and Alexa 568, Molecular Probes, Eugene, OR) labeled goat antimouse Ig antibodies.

    Techniques: Labeling, Confocal Microscopy

    Identification of Langerin + dendritic cells (DCs) in normal human sigmoid colon and rectum. Normal human sigmoid colon (A) and rectum (B) were labeled with monoclonal antibodies (mAbs) for CD207 (Langerin) and CD1a and visualized using the immunoperoxidase

    Journal: AIDS Research and Human Retroviruses

    Article Title: Antigen-Presenting Cell Candidates for HIV-1 Transmission in Human Distal Colonic Mucosa Defined by CD207 Dendritic Cells and CD209 Macrophages

    doi: 10.1089/aid.2013.0145

    Figure Lengend Snippet: Identification of Langerin + dendritic cells (DCs) in normal human sigmoid colon and rectum. Normal human sigmoid colon (A) and rectum (B) were labeled with monoclonal antibodies (mAbs) for CD207 (Langerin) and CD1a and visualized using the immunoperoxidase

    Article Snippet: Indirect immunofluorescence was performed by serially incubating cryostat-prepared tissue sections with mouse antihuman monoclonal antibodies (mAbs) to CD1a, CD207, CD209, CD68, and CD163 of different isotypes followed by incubation with isotype-specific fluorochrome- (Alexa 488 and Alexa 568, Molecular Probes, Eugene, OR) labeled goat antimouse Ig antibodies.

    Techniques: Labeling

    Morphology and distribution of monocyte/macrophage cell markers in normal human sigmoid colon and rectum. Normal human sigmoid colon and rectum were labeled with mAbs for CD14, CD68, CD163, and CD209, and were visualized using the immunoperoxidase method.

    Journal: AIDS Research and Human Retroviruses

    Article Title: Antigen-Presenting Cell Candidates for HIV-1 Transmission in Human Distal Colonic Mucosa Defined by CD207 Dendritic Cells and CD209 Macrophages

    doi: 10.1089/aid.2013.0145

    Figure Lengend Snippet: Morphology and distribution of monocyte/macrophage cell markers in normal human sigmoid colon and rectum. Normal human sigmoid colon and rectum were labeled with mAbs for CD14, CD68, CD163, and CD209, and were visualized using the immunoperoxidase method.

    Article Snippet: Indirect immunofluorescence was performed by serially incubating cryostat-prepared tissue sections with mouse antihuman monoclonal antibodies (mAbs) to CD1a, CD207, CD209, CD68, and CD163 of different isotypes followed by incubation with isotype-specific fluorochrome- (Alexa 488 and Alexa 568, Molecular Probes, Eugene, OR) labeled goat antimouse Ig antibodies.

    Techniques: Labeling

    Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for HCMV IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL mAb 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p

    Journal: Vaccines

    Article Title: Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas

    doi: 10.3390/vaccines7040135

    Figure Lengend Snippet: Antibody treatment of VR1814-infected anchoring villus explants postinfection reduces apoptosis in CTB cell columns. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunostaining for HCMV IE1 in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL mAb 2-18, and ( D ) 1.0 µg/mL mAb 3-25. Scale bar, panel C = 50 µm. Nuclei were stained with DAPI. ( E ) Graph showing the aggregate percentages of TUNEL-positive cell column CTBs (dark blue bars) and means of the percentages of TUNEL-positive CTBs in individual cell columns (light blue bars), along with standard deviations. * p

    Article Snippet: Antibodies and Reagents The following antibodies were purchased: mouse mAbs to HCMV IE (MilliporeSigma, Burlington, MA, USA), CD68 and CK7 (Dako, Carpinteria, CA, USA), and CD32A (Abcam, Cambridge, UK); a rabbit mAb to CD163 (Novus Biologicals, Centennial, CO, USA); and a rabbit polyclonal antibody to human cytokeratin 19 (ProteinTech, Rosemount, IL, USA).

    Techniques: Infection, CtB Assay, TUNEL Assay, Immunostaining, Staining

    Human monoclonal antibody (mAb) neutralization of VR1814 infection of primary human placental cells. Primary placental cells were infected with VR1814 preincubated with medium alone (no antibody control) or medium containing antibodies. Cells were fixed at 2 days postinfection and immunostained for human cytomegalovirus (HCMV) immediate-early protein 1 (IE1), and IE1+ cells were counted. Counts were normalized to the no antibody control and are expressed as percentages (mean ± SE). ( A ) Results from four independent experiments ( n = 2–8) on human placental fibroblasts (HPFs) from an 8-week gestation placenta. ( B ) Results from four independent experiments ( n = 8) on trophoblast progenitor cells (TBPCs) from a 15.6-week gestation placenta. ( C ) Results from three independent experiments ( n = 2–6) on amniotic epithelial cells (AmEpCs) from a 23.3-week gestation placenta. ( D ) Results from two independent experiments ( n = 2–4) on AmEpCs from a 38.6-week gestation placenta. Red boxes and arrows highlight the most potent neutralizing activities. n = total replicates counted across all experiments. GA, gestational age.

    Journal: Vaccines

    Article Title: Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas

    doi: 10.3390/vaccines7040135

    Figure Lengend Snippet: Human monoclonal antibody (mAb) neutralization of VR1814 infection of primary human placental cells. Primary placental cells were infected with VR1814 preincubated with medium alone (no antibody control) or medium containing antibodies. Cells were fixed at 2 days postinfection and immunostained for human cytomegalovirus (HCMV) immediate-early protein 1 (IE1), and IE1+ cells were counted. Counts were normalized to the no antibody control and are expressed as percentages (mean ± SE). ( A ) Results from four independent experiments ( n = 2–8) on human placental fibroblasts (HPFs) from an 8-week gestation placenta. ( B ) Results from four independent experiments ( n = 8) on trophoblast progenitor cells (TBPCs) from a 15.6-week gestation placenta. ( C ) Results from three independent experiments ( n = 2–6) on amniotic epithelial cells (AmEpCs) from a 23.3-week gestation placenta. ( D ) Results from two independent experiments ( n = 2–4) on AmEpCs from a 38.6-week gestation placenta. Red boxes and arrows highlight the most potent neutralizing activities. n = total replicates counted across all experiments. GA, gestational age.

    Article Snippet: Antibodies and Reagents The following antibodies were purchased: mouse mAbs to HCMV IE (MilliporeSigma, Burlington, MA, USA), CD68 and CK7 (Dako, Carpinteria, CA, USA), and CD32A (Abcam, Cambridge, UK); a rabbit mAb to CD163 (Novus Biologicals, Centennial, CO, USA); and a rabbit polyclonal antibody to human cytokeratin 19 (ProteinTech, Rosemount, IL, USA).

    Techniques: Neutralization, Infection

    mAbs specific to gB, gH/gL, and the pentamer complex neutralize infection of CTB cell columns. Quantification of antibody neutralizing activities on anchoring villus explants from four placentas of 8 ( A ), 11 ( B ), 9 ( C ), and 14 weeks ( D ) gestational age (GA) corresponding to explants shown in Figure 2 . Explants were infected with VR1814 preincubated with medium alone or with antibodies (i.e., immune complexes) at the concentrations indicated, and 341 cell columns were analyzed. Bars indicate aggregate percentage of cell column CTBs infected (HCMV IE1+) among all cell columns analyzed. Numbers of cell columns counted is indicated below each bar. Green boxes highlight the most potent neutralizing antibody.

    Journal: Vaccines

    Article Title: Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas

    doi: 10.3390/vaccines7040135

    Figure Lengend Snippet: mAbs specific to gB, gH/gL, and the pentamer complex neutralize infection of CTB cell columns. Quantification of antibody neutralizing activities on anchoring villus explants from four placentas of 8 ( A ), 11 ( B ), 9 ( C ), and 14 weeks ( D ) gestational age (GA) corresponding to explants shown in Figure 2 . Explants were infected with VR1814 preincubated with medium alone or with antibodies (i.e., immune complexes) at the concentrations indicated, and 341 cell columns were analyzed. Bars indicate aggregate percentage of cell column CTBs infected (HCMV IE1+) among all cell columns analyzed. Numbers of cell columns counted is indicated below each bar. Green boxes highlight the most potent neutralizing antibody.

    Article Snippet: Antibodies and Reagents The following antibodies were purchased: mouse mAbs to HCMV IE (MilliporeSigma, Burlington, MA, USA), CD68 and CK7 (Dako, Carpinteria, CA, USA), and CD32A (Abcam, Cambridge, UK); a rabbit mAb to CD163 (Novus Biologicals, Centennial, CO, USA); and a rabbit polyclonal antibody to human cytokeratin 19 (ProteinTech, Rosemount, IL, USA).

    Techniques: Infection, CtB Assay

    Hofbauer cells in villus cores phagocytose IgG-virion immune complexes transcytosed by syncytiotrophoblasts in anchoring villus explants. ( A – G ) Immunofluorescence staining for HCMV gB and CD68, a marker of Hofbauer cells, in sections of anchoring villus explants from an 8-week gestation placenta infected with VR1814 alone or mixed with antibodies (immune complexes). Cell column CTBs ( A ) and Hofbauer cells in the corresponding villus core ( B ) infected with VR1814 alone. Cell column CTBs ( C ) and Hofbauer cells in the corresponding villus core ( D , E ) infected with VR1814 mixed with 10 µg/mL Cytogam. Cell column CTBs ( F ) and Hofbauer cells in the corresponding villus core ( G ) infected with VR1814 mixed with 0.01 µg/mL mAb 2-18 (yellow frame). Immunofluorescence staining of villus explants from a 14-week gestation placenta for FcγRIIA and Hofbauer cell marker CD163 ( H ) and for FcγRIIA and FcRn ( I ). Scale bar for panels ( A – D ) and ( F – I ) in panel B = 20 µm. Scale bar, panel E = 100 µm. Nuclei were stained with DAPI. Similar results were seen in explants from 11 weeks’ gestation (data not shown).

    Journal: Vaccines

    Article Title: Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas

    doi: 10.3390/vaccines7040135

    Figure Lengend Snippet: Hofbauer cells in villus cores phagocytose IgG-virion immune complexes transcytosed by syncytiotrophoblasts in anchoring villus explants. ( A – G ) Immunofluorescence staining for HCMV gB and CD68, a marker of Hofbauer cells, in sections of anchoring villus explants from an 8-week gestation placenta infected with VR1814 alone or mixed with antibodies (immune complexes). Cell column CTBs ( A ) and Hofbauer cells in the corresponding villus core ( B ) infected with VR1814 alone. Cell column CTBs ( C ) and Hofbauer cells in the corresponding villus core ( D , E ) infected with VR1814 mixed with 10 µg/mL Cytogam. Cell column CTBs ( F ) and Hofbauer cells in the corresponding villus core ( G ) infected with VR1814 mixed with 0.01 µg/mL mAb 2-18 (yellow frame). Immunofluorescence staining of villus explants from a 14-week gestation placenta for FcγRIIA and Hofbauer cell marker CD163 ( H ) and for FcγRIIA and FcRn ( I ). Scale bar for panels ( A – D ) and ( F – I ) in panel B = 20 µm. Scale bar, panel E = 100 µm. Nuclei were stained with DAPI. Similar results were seen in explants from 11 weeks’ gestation (data not shown).

    Article Snippet: Antibodies and Reagents The following antibodies were purchased: mouse mAbs to HCMV IE (MilliporeSigma, Burlington, MA, USA), CD68 and CK7 (Dako, Carpinteria, CA, USA), and CD32A (Abcam, Cambridge, UK); a rabbit mAb to CD163 (Novus Biologicals, Centennial, CO, USA); and a rabbit polyclonal antibody to human cytokeratin 19 (ProteinTech, Rosemount, IL, USA).

    Techniques: Immunofluorescence, Staining, Marker, Infection

    Antibody treatment reduces gB expression and pUL112-113 in nuclear inclusions. Immunofluorescence staining for HCMV pUL112-113 and gB in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL mAb 2-18, and ( D ) 1.0 µg/mL mAb 3-25. ( E ) Percentages of gB-expressing cells with pUL112-113 nuclear inclusions versus punctate signal, including both aggregate percentages (dark blue bars) and means of the percentages in individual cell columns (light blue bars). Scale bar, panel C = 50 µm. Nuclei were stained with DAPI.

    Journal: Vaccines

    Article Title: Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas

    doi: 10.3390/vaccines7040135

    Figure Lengend Snippet: Antibody treatment reduces gB expression and pUL112-113 in nuclear inclusions. Immunofluorescence staining for HCMV pUL112-113 and gB in sections of anchoring villi described in Figure 5 . ( A ) Control untreated explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL mAb 2-18, and ( D ) 1.0 µg/mL mAb 3-25. ( E ) Percentages of gB-expressing cells with pUL112-113 nuclear inclusions versus punctate signal, including both aggregate percentages (dark blue bars) and means of the percentages in individual cell columns (light blue bars). Scale bar, panel C = 50 µm. Nuclei were stained with DAPI.

    Article Snippet: Antibodies and Reagents The following antibodies were purchased: mouse mAbs to HCMV IE (MilliporeSigma, Burlington, MA, USA), CD68 and CK7 (Dako, Carpinteria, CA, USA), and CD32A (Abcam, Cambridge, UK); a rabbit mAb to CD163 (Novus Biologicals, Centennial, CO, USA); and a rabbit polyclonal antibody to human cytokeratin 19 (ProteinTech, Rosemount, IL, USA).

    Techniques: Expressing, Immunofluorescence, Staining

    Neutralization of HCMV infection of cell column cytotrophoblasts (CTBs) in anchoring villus explants. Immunofluorescence staining for HCMV IE1 and cytokeratin (CK) (7D3) in sections of anchoring villus explants from four placentas of different gestational ages infected with VR1814 alone or mixed with antibodies (i.e., immune complexes) at the indicated concentrations. In two experiments (8 and 11 weeks’ gestation), single concentrations of mAbs were compared to single concentrations of Cytogam and the negative control antibody Synagis. In two experiments (9 and 14 weeks), multiple concentrations of mAbs 2-18, 3-16, and 3-25 were compared. Representative images of all conditions are shown for the 8-week placenta ( A – F ), whereas only results for VR1814 alone and VR1814 mixed with mAbs 2-18 and 3-25 are shown for explants from the 11-, 9-, and 14-week gestation placentas. ( G – O ) Quantitative results for all experimental conditions are shown in Figure 3 . ( A – F ) Explants from an 8-week gestation placenta showing infection with VR1814 alone ( A ) and infection with VR1814 mixed with negative control antibody Synagis (10 µg/mL; B ), Cytogam (10 µg/mL; C ), mAb 3-16 (1.0 µg/mL; D ), mAb 2-18 (0.01 µg/mL; E ), and mAb 3-25 (1.0 µg/mL; F ). ( G – O ) Selected images from neutralization studies in explants from three placentas showing infection alone ( G , J , M ) and infection with virus mixed with either mAb 2-18 (0.01 µg/mL; H , K , N ) or mAb 3-25 ( I , L , O ). Scale bar, panel C = 100 µm. Nuclei were stained with DAPI.

    Journal: Vaccines

    Article Title: Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas

    doi: 10.3390/vaccines7040135

    Figure Lengend Snippet: Neutralization of HCMV infection of cell column cytotrophoblasts (CTBs) in anchoring villus explants. Immunofluorescence staining for HCMV IE1 and cytokeratin (CK) (7D3) in sections of anchoring villus explants from four placentas of different gestational ages infected with VR1814 alone or mixed with antibodies (i.e., immune complexes) at the indicated concentrations. In two experiments (8 and 11 weeks’ gestation), single concentrations of mAbs were compared to single concentrations of Cytogam and the negative control antibody Synagis. In two experiments (9 and 14 weeks), multiple concentrations of mAbs 2-18, 3-16, and 3-25 were compared. Representative images of all conditions are shown for the 8-week placenta ( A – F ), whereas only results for VR1814 alone and VR1814 mixed with mAbs 2-18 and 3-25 are shown for explants from the 11-, 9-, and 14-week gestation placentas. ( G – O ) Quantitative results for all experimental conditions are shown in Figure 3 . ( A – F ) Explants from an 8-week gestation placenta showing infection with VR1814 alone ( A ) and infection with VR1814 mixed with negative control antibody Synagis (10 µg/mL; B ), Cytogam (10 µg/mL; C ), mAb 3-16 (1.0 µg/mL; D ), mAb 2-18 (0.01 µg/mL; E ), and mAb 3-25 (1.0 µg/mL; F ). ( G – O ) Selected images from neutralization studies in explants from three placentas showing infection alone ( G , J , M ) and infection with virus mixed with either mAb 2-18 (0.01 µg/mL; H , K , N ) or mAb 3-25 ( I , L , O ). Scale bar, panel C = 100 µm. Nuclei were stained with DAPI.

    Article Snippet: Antibodies and Reagents The following antibodies were purchased: mouse mAbs to HCMV IE (MilliporeSigma, Burlington, MA, USA), CD68 and CK7 (Dako, Carpinteria, CA, USA), and CD32A (Abcam, Cambridge, UK); a rabbit mAb to CD163 (Novus Biologicals, Centennial, CO, USA); and a rabbit polyclonal antibody to human cytokeratin 19 (ProteinTech, Rosemount, IL, USA).

    Techniques: Neutralization, Infection, Immunofluorescence, Staining, Negative Control

    Anti-pentamer mAb 2-18 treatment postinfection reduces cell-cell virus spread in CTB cell columns. Immunofluorescence staining for HCMV IE proteins and cytokeratin (CK) 19 in sections of villus explants from a 10-week gestation placenta infected with VR1814 and treated postinfection with antibodies for two days. ( A ) Untreated VR1814-infected explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL mAb 2-18, ( D ) 1.0 µg/mL mAb 3-25, and ( E ) 1.0 µg/mL mAb 3-16. Scale bar, panel E = 100 µm. Nuclei were stained with DAPI.

    Journal: Vaccines

    Article Title: Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas

    doi: 10.3390/vaccines7040135

    Figure Lengend Snippet: Anti-pentamer mAb 2-18 treatment postinfection reduces cell-cell virus spread in CTB cell columns. Immunofluorescence staining for HCMV IE proteins and cytokeratin (CK) 19 in sections of villus explants from a 10-week gestation placenta infected with VR1814 and treated postinfection with antibodies for two days. ( A ) Untreated VR1814-infected explant. ( B ) Explants treated with 100 µg/mL Cytogam, ( C ) 0.01 µg/mL mAb 2-18, ( D ) 1.0 µg/mL mAb 3-25, and ( E ) 1.0 µg/mL mAb 3-16. Scale bar, panel E = 100 µm. Nuclei were stained with DAPI.

    Article Snippet: Antibodies and Reagents The following antibodies were purchased: mouse mAbs to HCMV IE (MilliporeSigma, Burlington, MA, USA), CD68 and CK7 (Dako, Carpinteria, CA, USA), and CD32A (Abcam, Cambridge, UK); a rabbit mAb to CD163 (Novus Biologicals, Centennial, CO, USA); and a rabbit polyclonal antibody to human cytokeratin 19 (ProteinTech, Rosemount, IL, USA).

    Techniques: CtB Assay, Immunofluorescence, Staining, Infection

    Mutagenesis study of K1 ANK2. (A) The amino acid sequence of K1 ANK2 is shown in an alignment with the synthetic ANK sequence from 1MJ0, with the secondary structure of the synthetic ANK labeled. The amino acids that were substituted into each K1 mutant are shown below the alignment. The nonconservative and conservative amino acid changes are shown in regular type and italics, respectively. The phenotypes of the mutants in HeLa and RK13 cells are summarized on the right. The mutants were constructed by homologous recombination of vK1L − C7L − and a plasmid encoding C-terminal V5-taged K1 and the GFP. (B) Steady-state level of K1 proteins in Vero cells that had been infected with K1L mutants. Vero cells were infected at an MOI of 5 PFU/cell. The level of K1 proteins at 8 hpi were determined by Western blotting with a monoclonal V5 antibody. On the same blot, the levels of VACV early protein E3 and host cell protein β-actin were also determined. β-Actin serves as a control for gel loading, whereas E3 serves as an indicator of VACV early protein expression. The molecular mass marker is indicated on the left in kilodaltons.

    Journal: Journal of Virology

    Article Title: Structure Function Studies of Vaccinia Virus Host Range Protein K1 Reveal a Novel Functional Surface for Ankyrin Repeat Proteins ▿

    doi: 10.1128/JVI.02332-09

    Figure Lengend Snippet: Mutagenesis study of K1 ANK2. (A) The amino acid sequence of K1 ANK2 is shown in an alignment with the synthetic ANK sequence from 1MJ0, with the secondary structure of the synthetic ANK labeled. The amino acids that were substituted into each K1 mutant are shown below the alignment. The nonconservative and conservative amino acid changes are shown in regular type and italics, respectively. The phenotypes of the mutants in HeLa and RK13 cells are summarized on the right. The mutants were constructed by homologous recombination of vK1L − C7L − and a plasmid encoding C-terminal V5-taged K1 and the GFP. (B) Steady-state level of K1 proteins in Vero cells that had been infected with K1L mutants. Vero cells were infected at an MOI of 5 PFU/cell. The level of K1 proteins at 8 hpi were determined by Western blotting with a monoclonal V5 antibody. On the same blot, the levels of VACV early protein E3 and host cell protein β-actin were also determined. β-Actin serves as a control for gel loading, whereas E3 serves as an indicator of VACV early protein expression. The molecular mass marker is indicated on the left in kilodaltons.

    Article Snippet: The detection antibodies were mouse monoclonal antibodies (MAbs) to V5 (Sigma-Aldrich; clone V5-10), MAbs to β-actin (Sigma-Aldrich), and rabbit anti-E3L polyclonal antiserum (kindly provided by Bertram Jacobs).

    Techniques: Mutagenesis, Sequencing, Labeling, Construct, Homologous Recombination, Plasmid Preparation, Infection, Western Blot, Expressing, Marker

    Immunohistochemistry representative of ovine L-BSE. All figures illustrate labelling with mAb 2G11, except B . Neuronal and particulate labelling is present in the DNV with mAb 2G11 ( A ), but absent with mAb P4 ( B ) (case 455/11). Particulate labelling and small aggregates are abundant in many areas, such as the thalamic nuclei ( C ) (case 1591/10). Perineuronal labelling in the putamen is a consistent and striking feature of ovine L-BSE ( D ) (case 1591/10), as is intracellular labelling of oligodendrocytes, seen here in the spinocerebellar tract (rostral medulla) ( E ) (case 58/11). Intraneuronal labelling is also present in the DRG ( F ) (case 4/12). Heavy labelling in muscle spindles is also visible ( G ) (case 267/11) and also in occasional myocytes ( H ) (case 98/11). Labelling was also present in the LRS ( I ) and ENS (J) of one VRQ/VRQ recipient (case 48/13).

    Journal: Veterinary Research

    Article Title: L-BSE experimentally transmitted to sheep presents as a unique disease phenotype

    doi: 10.1186/s13567-016-0394-1

    Figure Lengend Snippet: Immunohistochemistry representative of ovine L-BSE. All figures illustrate labelling with mAb 2G11, except B . Neuronal and particulate labelling is present in the DNV with mAb 2G11 ( A ), but absent with mAb P4 ( B ) (case 455/11). Particulate labelling and small aggregates are abundant in many areas, such as the thalamic nuclei ( C ) (case 1591/10). Perineuronal labelling in the putamen is a consistent and striking feature of ovine L-BSE ( D ) (case 1591/10), as is intracellular labelling of oligodendrocytes, seen here in the spinocerebellar tract (rostral medulla) ( E ) (case 58/11). Intraneuronal labelling is also present in the DRG ( F ) (case 4/12). Heavy labelling in muscle spindles is also visible ( G ) (case 267/11) and also in occasional myocytes ( H ) (case 98/11). Labelling was also present in the LRS ( I ) and ENS (J) of one VRQ/VRQ recipient (case 48/13).

    Article Snippet: Immunohistochemical detection of PrPSc was performed on adjacent sections from the same brain blocks, and on the other tissues, using mouse monoclonal antibody (mAb) 2G11 (ABD Serotec), raised against the ovine PrP peptide sequence 146-R154 R171 -182, as described in detail elsewhere [ ].

    Techniques: Immunohistochemistry