mouse monoclonal anti ace2 ab Search Results


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    Santa Cruz Biotechnology mouse monoclonal anti ace2
    Distribution of <t>ACE2</t> receptor and attachment of SARS-CoV-2 and human coronavirus (HCoV-NL63) to tissue sections of human trachea. These images show formalin-fixed paraffin-embedded cross-sections of human tracheal sections stained with ImmPRESS VR anti-mouse/rabbit IgG horseradish peroxidase (HRP) polymer detection kit. Dark brown represents the presence of a protein interacting with a specific antibody and is considered a positive expression. Pale brown background, and the nucleus counterstained with hematoxylin is blue. ( a ) Expression of ACE2 in human trachea revealed by immunostaining with mouse anti-ACE2 monoclonal antibody (4 μg/mL); and ( d ) corresponding negative control tissue sections stained with secondary anti-mouse HRP antibody only. ( b , c , e , f ) The tissues sections presented in this panel show virus binding in formalin-fixed paraffin-embedded cross-sections after overnight incubation with 250 μL ( b ) heat-inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020 or ( c ) HCoV-NL63. ( e , f ) Virus-incubated sections that were stained with secondary anti-rabbit HRP antibody only. Scale bar—100 μm.
    Mouse Monoclonal Anti Ace2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti ace2/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti ace2 - by Bioz Stars, 2024-07
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    86
    Thermo Fisher mouse monoclonal anti ace2
    <t>ACE2</t> mRNA expression in the thyroid series. Analysis of ACE2 mRNA expression according to the ( a ) diagnosis; ( b ) histotype; ( c ) subtype. Results are shown as median ± IQR. ** p -value ≤ 0.01 and *** p -value ≤ 0.001. Abbreviations: AT (Adjacent Thyroid Tissues), FTA (Follicular Thyroid Adenomas), PTC (Papillary Thyroid Carcinomas), FTC (Follicular Thyroid Carcinomas), PDTC (Poorly Differentiated Thyroid Carcinomas), cPTC (classical Papillary Thyroid Carcinomas), FVPTC (Follicular Variant of Papillary Thyroid Carcinomas) and DSVPTC (Diffuse Sclerosing Papillary Thyroid Carcinomas).
    Mouse Monoclonal Anti Ace2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti ace2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti ace2 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher mouse monoclonal anti ace 2
    (A) <t>ACE-2</t> expression in a thyroid specimen with extensive staining, and (B) in the lung, with staining in <10% of the cells (arrows) (scale bar: 100 μm); (C) TMPRSS2 expression in the thyrocytes with strong cytoplasmatic expression (scale bar: 100 μm) and (D) in the lung with strong cytoplasmatic/nuclear staining (scale bar: 100 μm); (E) Furin moderates cytoplasmatic expression in the thyrocytes (scale bar: 100 μm) and (F) in the lung with low cytoplasmatic staining (arrows) (scale bar: 100 μm).
    Mouse Monoclonal Anti Ace 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti ace 2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti ace 2 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher mouse anti ace2 monoclonal antibody
    Docking score of the ten compounds selected from our in-house library, and ponatinib, for the <t> ACE2 </t> binding pocket (6M18) and the RBD of the spike protein (6M0J), calculated using Autodock Vina.
    Mouse Anti Ace2 Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ace2 monoclonal antibody/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti ace2 monoclonal antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Enzo Biochem mouse monoclonal anti ace2 antibody
    Docking score of the ten compounds selected from our in-house library, and ponatinib, for the <t> ACE2 </t> binding pocket (6M18) and the RBD of the spike protein (6M0J), calculated using Autodock Vina.
    Mouse Monoclonal Anti Ace2 Antibody, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti ace2 antibody/product/Enzo Biochem
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti ace2 antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Distribution of ACE2 receptor and attachment of SARS-CoV-2 and human coronavirus (HCoV-NL63) to tissue sections of human trachea. These images show formalin-fixed paraffin-embedded cross-sections of human tracheal sections stained with ImmPRESS VR anti-mouse/rabbit IgG horseradish peroxidase (HRP) polymer detection kit. Dark brown represents the presence of a protein interacting with a specific antibody and is considered a positive expression. Pale brown background, and the nucleus counterstained with hematoxylin is blue. ( a ) Expression of ACE2 in human trachea revealed by immunostaining with mouse anti-ACE2 monoclonal antibody (4 μg/mL); and ( d ) corresponding negative control tissue sections stained with secondary anti-mouse HRP antibody only. ( b , c , e , f ) The tissues sections presented in this panel show virus binding in formalin-fixed paraffin-embedded cross-sections after overnight incubation with 250 μL ( b ) heat-inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020 or ( c ) HCoV-NL63. ( e , f ) Virus-incubated sections that were stained with secondary anti-rabbit HRP antibody only. Scale bar—100 μm.

    Journal: Viruses

    Article Title: SARS-CoV-2 Is More Efficient than HCoV-NL63 in Infecting a Small Subpopulation of ACE2+ Human Respiratory Epithelial Cells

    doi: 10.3390/v15030736

    Figure Lengend Snippet: Distribution of ACE2 receptor and attachment of SARS-CoV-2 and human coronavirus (HCoV-NL63) to tissue sections of human trachea. These images show formalin-fixed paraffin-embedded cross-sections of human tracheal sections stained with ImmPRESS VR anti-mouse/rabbit IgG horseradish peroxidase (HRP) polymer detection kit. Dark brown represents the presence of a protein interacting with a specific antibody and is considered a positive expression. Pale brown background, and the nucleus counterstained with hematoxylin is blue. ( a ) Expression of ACE2 in human trachea revealed by immunostaining with mouse anti-ACE2 monoclonal antibody (4 μg/mL); and ( d ) corresponding negative control tissue sections stained with secondary anti-mouse HRP antibody only. ( b , c , e , f ) The tissues sections presented in this panel show virus binding in formalin-fixed paraffin-embedded cross-sections after overnight incubation with 250 μL ( b ) heat-inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020 or ( c ) HCoV-NL63. ( e , f ) Virus-incubated sections that were stained with secondary anti-rabbit HRP antibody only. Scale bar—100 μm.

    Article Snippet: The following primary antibodies were used in this study: mouse monoclonal anti-ACE2 (4 μg/mL; E-11 Santa Cruz Biotechnology, Dallas, TX, USA); mouse anti-pan-cytokeratin (0.5 μg/mL; AE1/AE3; Bio-Rad Laboratories, Hercules, CA, USA); anti-HCoV-NL63 nucleocapsid (N) protein monoclonal antibody (2D4; Ingenasa-Eurofins, Madrid, Spain) (0.25 µg/mL) and a recombinant anti-SARS-CoV-2 nucleocapsid (N) protein rabbit monoclonal antibody (0.75 μg/mL) (BEI Resources) [The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-SARS Coronavirus/SARS-Related Coronavirus 2 Nucleocapsid Protein (produced in vitro), NR-53791; SinoBio Cat: 40143-R001].

    Techniques: Formalin-fixed Paraffin-Embedded, Staining, Expressing, Immunostaining, Negative Control, Binding Assay, Incubation

    Characterization of human respiratory epithelial cells (HRECs) for pan-cytokeratin and angiotensin-converting enzyme-2 (ACE2) using target-specific markers. Primary HRECs stained for mouse monoclonal to anti-pan-cytokeratin (epithelial cell marker; 0.5 μg/mL) showed strong expression both in ( a ) IHC and ( c ) flow cytometry analysis. ( b ) HRECs incubated with a secondary antibody only displayed minimum background. ( c ) HRECs were also quantified for the ACE2 expression using flow cytometry. Flow cytometry data was collected using an Attune NxT flow cytometer. A representative of 10,000 events were acquired and analyzed for each sample. Cells were gated for singlet population using forward (FSA) and side-scatter (SSA) properties, and the mean of percent live cell population was used to quantify the levels of ( c-i ) pan-cytokeratin and ( c-ii ) ACE2 ( n = 4). The bar graph represents the mean with the standard error of the mean (SEM).

    Journal: Viruses

    Article Title: SARS-CoV-2 Is More Efficient than HCoV-NL63 in Infecting a Small Subpopulation of ACE2+ Human Respiratory Epithelial Cells

    doi: 10.3390/v15030736

    Figure Lengend Snippet: Characterization of human respiratory epithelial cells (HRECs) for pan-cytokeratin and angiotensin-converting enzyme-2 (ACE2) using target-specific markers. Primary HRECs stained for mouse monoclonal to anti-pan-cytokeratin (epithelial cell marker; 0.5 μg/mL) showed strong expression both in ( a ) IHC and ( c ) flow cytometry analysis. ( b ) HRECs incubated with a secondary antibody only displayed minimum background. ( c ) HRECs were also quantified for the ACE2 expression using flow cytometry. Flow cytometry data was collected using an Attune NxT flow cytometer. A representative of 10,000 events were acquired and analyzed for each sample. Cells were gated for singlet population using forward (FSA) and side-scatter (SSA) properties, and the mean of percent live cell population was used to quantify the levels of ( c-i ) pan-cytokeratin and ( c-ii ) ACE2 ( n = 4). The bar graph represents the mean with the standard error of the mean (SEM).

    Article Snippet: The following primary antibodies were used in this study: mouse monoclonal anti-ACE2 (4 μg/mL; E-11 Santa Cruz Biotechnology, Dallas, TX, USA); mouse anti-pan-cytokeratin (0.5 μg/mL; AE1/AE3; Bio-Rad Laboratories, Hercules, CA, USA); anti-HCoV-NL63 nucleocapsid (N) protein monoclonal antibody (2D4; Ingenasa-Eurofins, Madrid, Spain) (0.25 µg/mL) and a recombinant anti-SARS-CoV-2 nucleocapsid (N) protein rabbit monoclonal antibody (0.75 μg/mL) (BEI Resources) [The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-SARS Coronavirus/SARS-Related Coronavirus 2 Nucleocapsid Protein (produced in vitro), NR-53791; SinoBio Cat: 40143-R001].

    Techniques: Staining, Marker, Expressing, Flow Cytometry, Incubation

    ACE2 mRNA expression in the thyroid series. Analysis of ACE2 mRNA expression according to the ( a ) diagnosis; ( b ) histotype; ( c ) subtype. Results are shown as median ± IQR. ** p -value ≤ 0.01 and *** p -value ≤ 0.001. Abbreviations: AT (Adjacent Thyroid Tissues), FTA (Follicular Thyroid Adenomas), PTC (Papillary Thyroid Carcinomas), FTC (Follicular Thyroid Carcinomas), PDTC (Poorly Differentiated Thyroid Carcinomas), cPTC (classical Papillary Thyroid Carcinomas), FVPTC (Follicular Variant of Papillary Thyroid Carcinomas) and DSVPTC (Diffuse Sclerosing Papillary Thyroid Carcinomas).

    Journal: Cancers

    Article Title: Significance of Furin Expression in Thyroid Neoplastic Transformation

    doi: 10.3390/cancers15153909

    Figure Lengend Snippet: ACE2 mRNA expression in the thyroid series. Analysis of ACE2 mRNA expression according to the ( a ) diagnosis; ( b ) histotype; ( c ) subtype. Results are shown as median ± IQR. ** p -value ≤ 0.01 and *** p -value ≤ 0.001. Abbreviations: AT (Adjacent Thyroid Tissues), FTA (Follicular Thyroid Adenomas), PTC (Papillary Thyroid Carcinomas), FTC (Follicular Thyroid Carcinomas), PDTC (Poorly Differentiated Thyroid Carcinomas), cPTC (classical Papillary Thyroid Carcinomas), FVPTC (Follicular Variant of Papillary Thyroid Carcinomas) and DSVPTC (Diffuse Sclerosing Papillary Thyroid Carcinomas).

    Article Snippet: Mouse monoclonal anti-ACE2 (Invitrogen, Waltham, MA, USA, MA5-31395, 1:2500); rabbit monoclonal anti-TMPRSS2 antibody (Abcam, Boston, MA, USA, ab109131, clone EPR3862, 1:1000); and rabbit polyclonal anti-Furin (Invitrogen, PA5-96680; 1:250) were used.

    Techniques: Expressing, Variant Assay

    ACE2 mRNA expression correlation with clinicopathological data. Analysis of ACE2 mRNA expression in FTAs comparing ( a ) tumour size; ( b ) presence of lymphocytic infiltration; ( c ) presence of CLT and ( d ) TERT expression. Results are shown as median ± IQR. * p -value ≤ 0.05 and ** p -value ≤ 0.01. Abbreviations: CLT (Chronic lymphocytic thyroiditis).

    Journal: Cancers

    Article Title: Significance of Furin Expression in Thyroid Neoplastic Transformation

    doi: 10.3390/cancers15153909

    Figure Lengend Snippet: ACE2 mRNA expression correlation with clinicopathological data. Analysis of ACE2 mRNA expression in FTAs comparing ( a ) tumour size; ( b ) presence of lymphocytic infiltration; ( c ) presence of CLT and ( d ) TERT expression. Results are shown as median ± IQR. * p -value ≤ 0.05 and ** p -value ≤ 0.01. Abbreviations: CLT (Chronic lymphocytic thyroiditis).

    Article Snippet: Mouse monoclonal anti-ACE2 (Invitrogen, Waltham, MA, USA, MA5-31395, 1:2500); rabbit monoclonal anti-TMPRSS2 antibody (Abcam, Boston, MA, USA, ab109131, clone EPR3862, 1:1000); and rabbit polyclonal anti-Furin (Invitrogen, PA5-96680; 1:250) were used.

    Techniques: Expressing

    mRNA expression distribution in adjacent thyroid and thyroid neoplasms. Analysis of mRNA expression distribution of ACE2 ( Top Panel), TMPRSS2 ( Middle Panel), and Furin ( Lower Panel).

    Journal: Cancers

    Article Title: Significance of Furin Expression in Thyroid Neoplastic Transformation

    doi: 10.3390/cancers15153909

    Figure Lengend Snippet: mRNA expression distribution in adjacent thyroid and thyroid neoplasms. Analysis of mRNA expression distribution of ACE2 ( Top Panel), TMPRSS2 ( Middle Panel), and Furin ( Lower Panel).

    Article Snippet: Mouse monoclonal anti-ACE2 (Invitrogen, Waltham, MA, USA, MA5-31395, 1:2500); rabbit monoclonal anti-TMPRSS2 antibody (Abcam, Boston, MA, USA, ab109131, clone EPR3862, 1:1000); and rabbit polyclonal anti-Furin (Invitrogen, PA5-96680; 1:250) were used.

    Techniques: Expressing

    ROC curve analysis. ( a ) AUC representation for ACE2 , TMPRSS2 , and Furin mRNA expression in adjacent thyroid tissues and thyroid neoplasms; ( b ) Furin AUC when data were restricted to PTCs; ( c ) AUC values for ACE2 , TMPRSS2, and Furin mRNA expression in both analyses. Abbreviations: AUC (area under the curve).

    Journal: Cancers

    Article Title: Significance of Furin Expression in Thyroid Neoplastic Transformation

    doi: 10.3390/cancers15153909

    Figure Lengend Snippet: ROC curve analysis. ( a ) AUC representation for ACE2 , TMPRSS2 , and Furin mRNA expression in adjacent thyroid tissues and thyroid neoplasms; ( b ) Furin AUC when data were restricted to PTCs; ( c ) AUC values for ACE2 , TMPRSS2, and Furin mRNA expression in both analyses. Abbreviations: AUC (area under the curve).

    Article Snippet: Mouse monoclonal anti-ACE2 (Invitrogen, Waltham, MA, USA, MA5-31395, 1:2500); rabbit monoclonal anti-TMPRSS2 antibody (Abcam, Boston, MA, USA, ab109131, clone EPR3862, 1:1000); and rabbit polyclonal anti-Furin (Invitrogen, PA5-96680; 1:250) were used.

    Techniques: Expressing

    The immunohistochemical staining pattern of ACE2, TMPRSS2, and Furin. Representative images of ACE2 staining in ( a ) adjacent thyroid tissue and ( b ) follicular variant of PTC. The staining is mainly membranous, located in endothelial cells (arrowheads). Representative images of TMPRSS2 staining in ( c ) AT and ( d ) cPTC. The staining is mainly cytoplasmic (asterisks), and occasionally nuclear (arrows), located in follicular cells. Representative images of Furin staining in ( e ) AT, ( f ) FTA, and ( g ) cPTC. The staining is both cytoplasmic (asterisks) and nuclear (arrows), located in follicular cells. Scale bars: 50 μm. Abbreviations: AT (Adjacent Thyroid Tissue), FTA (Follicular Thyroid Adenoma), and cPTC (classical Papillary Thyroid Carcinoma).

    Journal: Cancers

    Article Title: Significance of Furin Expression in Thyroid Neoplastic Transformation

    doi: 10.3390/cancers15153909

    Figure Lengend Snippet: The immunohistochemical staining pattern of ACE2, TMPRSS2, and Furin. Representative images of ACE2 staining in ( a ) adjacent thyroid tissue and ( b ) follicular variant of PTC. The staining is mainly membranous, located in endothelial cells (arrowheads). Representative images of TMPRSS2 staining in ( c ) AT and ( d ) cPTC. The staining is mainly cytoplasmic (asterisks), and occasionally nuclear (arrows), located in follicular cells. Representative images of Furin staining in ( e ) AT, ( f ) FTA, and ( g ) cPTC. The staining is both cytoplasmic (asterisks) and nuclear (arrows), located in follicular cells. Scale bars: 50 μm. Abbreviations: AT (Adjacent Thyroid Tissue), FTA (Follicular Thyroid Adenoma), and cPTC (classical Papillary Thyroid Carcinoma).

    Article Snippet: Mouse monoclonal anti-ACE2 (Invitrogen, Waltham, MA, USA, MA5-31395, 1:2500); rabbit monoclonal anti-TMPRSS2 antibody (Abcam, Boston, MA, USA, ab109131, clone EPR3862, 1:1000); and rabbit polyclonal anti-Furin (Invitrogen, PA5-96680; 1:250) were used.

    Techniques: Immunohistochemical staining, Staining, Variant Assay

    (A) ACE-2 expression in a thyroid specimen with extensive staining, and (B) in the lung, with staining in <10% of the cells (arrows) (scale bar: 100 μm); (C) TMPRSS2 expression in the thyrocytes with strong cytoplasmatic expression (scale bar: 100 μm) and (D) in the lung with strong cytoplasmatic/nuclear staining (scale bar: 100 μm); (E) Furin moderates cytoplasmatic expression in the thyrocytes (scale bar: 100 μm) and (F) in the lung with low cytoplasmatic staining (arrows) (scale bar: 100 μm).

    Journal: European Thyroid Journal

    Article Title: Detection of SARS-CoV-2 infection in thyroid follicular cells from a COVID-19 autopsy series

    doi: 10.1530/ETJ-22-0074

    Figure Lengend Snippet: (A) ACE-2 expression in a thyroid specimen with extensive staining, and (B) in the lung, with staining in <10% of the cells (arrows) (scale bar: 100 μm); (C) TMPRSS2 expression in the thyrocytes with strong cytoplasmatic expression (scale bar: 100 μm) and (D) in the lung with strong cytoplasmatic/nuclear staining (scale bar: 100 μm); (E) Furin moderates cytoplasmatic expression in the thyrocytes (scale bar: 100 μm) and (F) in the lung with low cytoplasmatic staining (arrows) (scale bar: 100 μm).

    Article Snippet: The antibodies used were: mouse monoclonal anti-SARS/SARS-CoV-2 coronavirus nucleocapsid antibody (Invitrogen, MA1-7404, 1:100); mouse monoclonal SARS-CoV/SARS-CoV-2 spike antibody ((1A9), GTX632604, Genetex (Irvine, CA, USA), 1:200); mouse monoclonal anti-ACE-2 (Invitrogen, MA5-31395, 1:2500); rabbit monoclonal anti-TMPRSS2 antibody ((EPR3862), Abcam, ab109131, 1:1000); rabbit polyclonal anti-furin (Invitrogen, PA5-96680; 1:200); and rabbit monoclonal anti-cleaved caspase-3 (Asp175, Cell Signalling, 9664, 1:1000).

    Techniques: Expressing, Staining

    Docking score of the ten compounds selected from our in-house library, and ponatinib, for the  ACE2  binding pocket (6M18) and the RBD of the spike protein (6M0J), calculated using Autodock Vina.

    Journal: Molecules

    Article Title: Evaluation of the Cytotoxic and Antiviral Effects of Small Molecules Selected by In Silico Studies as Inhibitors of SARS-CoV-2 Cell Entry

    doi: 10.3390/molecules28207204

    Figure Lengend Snippet: Docking score of the ten compounds selected from our in-house library, and ponatinib, for the ACE2 binding pocket (6M18) and the RBD of the spike protein (6M0J), calculated using Autodock Vina.

    Article Snippet: Cell pellets were collected after 48 h incubation, and the following lysis, quantification, and Western Blot procedures were performed as previously described [ ], except for the following points: (i) no phosphatase inhibitor was used; (ii) a total of 15 μg of protein lysates obtained from MDA-MB-231 cells treatment or 50 μg of protein lysates obtained from Vero CCL-81 cells were loaded and separated in 10% SDS-Page gels; (iii) the transference to a nitrocellulose membrane (GE Healthcare Life science, Chalfont St Giles, UK; GE10600002) occurred for 1 h 45 min at 100 V; (iv) the primary antibodies (1:2000) used were: mouse anti-ACE2 monoclonal antibody (CL4035; Thermo Fischer Scientific; Waltham, MA, USA) and mouse anti-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Binding Assay

    In silico virtual screening. Top-ranked selected test compounds docked into ( A ) ACE2 and ( B ) the RBD of the spike protein. The compounds selected from the in silico studies are represented with sticks in different colors. Crystal structures were obtained from the Protein Data Bank (ACE2, PDB code 6M18 and the RBD of the spike protein, PDB code 6M0J ) and are represented as surface plots.

    Journal: Molecules

    Article Title: Evaluation of the Cytotoxic and Antiviral Effects of Small Molecules Selected by In Silico Studies as Inhibitors of SARS-CoV-2 Cell Entry

    doi: 10.3390/molecules28207204

    Figure Lengend Snippet: In silico virtual screening. Top-ranked selected test compounds docked into ( A ) ACE2 and ( B ) the RBD of the spike protein. The compounds selected from the in silico studies are represented with sticks in different colors. Crystal structures were obtained from the Protein Data Bank (ACE2, PDB code 6M18 and the RBD of the spike protein, PDB code 6M0J ) and are represented as surface plots.

    Article Snippet: Cell pellets were collected after 48 h incubation, and the following lysis, quantification, and Western Blot procedures were performed as previously described [ ], except for the following points: (i) no phosphatase inhibitor was used; (ii) a total of 15 μg of protein lysates obtained from MDA-MB-231 cells treatment or 50 μg of protein lysates obtained from Vero CCL-81 cells were loaded and separated in 10% SDS-Page gels; (iii) the transference to a nitrocellulose membrane (GE Healthcare Life science, Chalfont St Giles, UK; GE10600002) occurred for 1 h 45 min at 100 V; (iv) the primary antibodies (1:2000) used were: mouse anti-ACE2 monoclonal antibody (CL4035; Thermo Fischer Scientific; Waltham, MA, USA) and mouse anti-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: In Silico

    Molecular visualization of sulfonamide xanthenes 1 and 2 in ACE2. ( A ) General view of compounds 1 (green) and 2 (brown). ( B ) Interaction between compound 1 and ACE2. ( C ) Interaction between compound 2 and ACE2. Polar interactions are represented as yellow dashes. Residues involved on those interactions are labeled: Ala—alanine; Asn—asparagine; Asp—aspartic acid; His—histidine; Tyr—tyrosine.

    Journal: Molecules

    Article Title: Evaluation of the Cytotoxic and Antiviral Effects of Small Molecules Selected by In Silico Studies as Inhibitors of SARS-CoV-2 Cell Entry

    doi: 10.3390/molecules28207204

    Figure Lengend Snippet: Molecular visualization of sulfonamide xanthenes 1 and 2 in ACE2. ( A ) General view of compounds 1 (green) and 2 (brown). ( B ) Interaction between compound 1 and ACE2. ( C ) Interaction between compound 2 and ACE2. Polar interactions are represented as yellow dashes. Residues involved on those interactions are labeled: Ala—alanine; Asn—asparagine; Asp—aspartic acid; His—histidine; Tyr—tyrosine.

    Article Snippet: Cell pellets were collected after 48 h incubation, and the following lysis, quantification, and Western Blot procedures were performed as previously described [ ], except for the following points: (i) no phosphatase inhibitor was used; (ii) a total of 15 μg of protein lysates obtained from MDA-MB-231 cells treatment or 50 μg of protein lysates obtained from Vero CCL-81 cells were loaded and separated in 10% SDS-Page gels; (iii) the transference to a nitrocellulose membrane (GE Healthcare Life science, Chalfont St Giles, UK; GE10600002) occurred for 1 h 45 min at 100 V; (iv) the primary antibodies (1:2000) used were: mouse anti-ACE2 monoclonal antibody (CL4035; Thermo Fischer Scientific; Waltham, MA, USA) and mouse anti-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Labeling

    Molecular visualization of bile acid derivative 4 in ( A ) ACE2 and ( B ) the RBD of the spike protein. Polar interactions are represented as yellow dashes. Residues involved on those interactions are labeled: Asn—asparagine; Asp—aspartic acid; His—histidine; Tyr—tyrosine.

    Journal: Molecules

    Article Title: Evaluation of the Cytotoxic and Antiviral Effects of Small Molecules Selected by In Silico Studies as Inhibitors of SARS-CoV-2 Cell Entry

    doi: 10.3390/molecules28207204

    Figure Lengend Snippet: Molecular visualization of bile acid derivative 4 in ( A ) ACE2 and ( B ) the RBD of the spike protein. Polar interactions are represented as yellow dashes. Residues involved on those interactions are labeled: Asn—asparagine; Asp—aspartic acid; His—histidine; Tyr—tyrosine.

    Article Snippet: Cell pellets were collected after 48 h incubation, and the following lysis, quantification, and Western Blot procedures were performed as previously described [ ], except for the following points: (i) no phosphatase inhibitor was used; (ii) a total of 15 μg of protein lysates obtained from MDA-MB-231 cells treatment or 50 μg of protein lysates obtained from Vero CCL-81 cells were loaded and separated in 10% SDS-Page gels; (iii) the transference to a nitrocellulose membrane (GE Healthcare Life science, Chalfont St Giles, UK; GE10600002) occurred for 1 h 45 min at 100 V; (iv) the primary antibodies (1:2000) used were: mouse anti-ACE2 monoclonal antibody (CL4035; Thermo Fischer Scientific; Waltham, MA, USA) and mouse anti-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Labeling

    Molecular visualization of derivatives glucosulfated xanthone 7 and 9 in ACE2. ( A ) General view of compounds 7 (yellow) and 9 (blue). ( B ) Interaction between compound 7 and residues of ACE2. ( C ) Interaction between compound 9 and residues of ACE2. Polar interactions are represented as yellow dashes. Residues involved on those interactions are labeled: Ala—alanine; Arg—arginine; Asp—aspartic acid; Gln—glutamine; Gly—glycine; NAG—N-acetylglucosamine; Thr—threonine; Tyr—tyrosine.

    Journal: Molecules

    Article Title: Evaluation of the Cytotoxic and Antiviral Effects of Small Molecules Selected by In Silico Studies as Inhibitors of SARS-CoV-2 Cell Entry

    doi: 10.3390/molecules28207204

    Figure Lengend Snippet: Molecular visualization of derivatives glucosulfated xanthone 7 and 9 in ACE2. ( A ) General view of compounds 7 (yellow) and 9 (blue). ( B ) Interaction between compound 7 and residues of ACE2. ( C ) Interaction between compound 9 and residues of ACE2. Polar interactions are represented as yellow dashes. Residues involved on those interactions are labeled: Ala—alanine; Arg—arginine; Asp—aspartic acid; Gln—glutamine; Gly—glycine; NAG—N-acetylglucosamine; Thr—threonine; Tyr—tyrosine.

    Article Snippet: Cell pellets were collected after 48 h incubation, and the following lysis, quantification, and Western Blot procedures were performed as previously described [ ], except for the following points: (i) no phosphatase inhibitor was used; (ii) a total of 15 μg of protein lysates obtained from MDA-MB-231 cells treatment or 50 μg of protein lysates obtained from Vero CCL-81 cells were loaded and separated in 10% SDS-Page gels; (iii) the transference to a nitrocellulose membrane (GE Healthcare Life science, Chalfont St Giles, UK; GE10600002) occurred for 1 h 45 min at 100 V; (iv) the primary antibodies (1:2000) used were: mouse anti-ACE2 monoclonal antibody (CL4035; Thermo Fischer Scientific; Waltham, MA, USA) and mouse anti-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Labeling

    Levels of ( A ) spike and ( B ) ACE2 proteins in Vero CCL-81 cells treated with the IC 50 concentrations of the tested compounds (or 50 μM for compound 9 ) and infected with SARS-CoV-2 (MOI = 1), analyzed by Western blot. Actin was used as a loading control. Representative blots are shown. Data represents the mean ± SEM of at least three independent experiments. Analysis was performed by GraphPad using the Student t -test. * p < 0.05; *** p < 0.001 relative to the vehicle (DMSO for compounds 1 , 2 and 4 ; H 2 O for compounds 7 and 9 ).

    Journal: Molecules

    Article Title: Evaluation of the Cytotoxic and Antiviral Effects of Small Molecules Selected by In Silico Studies as Inhibitors of SARS-CoV-2 Cell Entry

    doi: 10.3390/molecules28207204

    Figure Lengend Snippet: Levels of ( A ) spike and ( B ) ACE2 proteins in Vero CCL-81 cells treated with the IC 50 concentrations of the tested compounds (or 50 μM for compound 9 ) and infected with SARS-CoV-2 (MOI = 1), analyzed by Western blot. Actin was used as a loading control. Representative blots are shown. Data represents the mean ± SEM of at least three independent experiments. Analysis was performed by GraphPad using the Student t -test. * p < 0.05; *** p < 0.001 relative to the vehicle (DMSO for compounds 1 , 2 and 4 ; H 2 O for compounds 7 and 9 ).

    Article Snippet: Cell pellets were collected after 48 h incubation, and the following lysis, quantification, and Western Blot procedures were performed as previously described [ ], except for the following points: (i) no phosphatase inhibitor was used; (ii) a total of 15 μg of protein lysates obtained from MDA-MB-231 cells treatment or 50 μg of protein lysates obtained from Vero CCL-81 cells were loaded and separated in 10% SDS-Page gels; (iii) the transference to a nitrocellulose membrane (GE Healthcare Life science, Chalfont St Giles, UK; GE10600002) occurred for 1 h 45 min at 100 V; (iv) the primary antibodies (1:2000) used were: mouse anti-ACE2 monoclonal antibody (CL4035; Thermo Fischer Scientific; Waltham, MA, USA) and mouse anti-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Infection, Western Blot

    Summary of the results obtained for compounds 1 , 2 , 4 , 7 , and 9 .

    Journal: Molecules

    Article Title: Evaluation of the Cytotoxic and Antiviral Effects of Small Molecules Selected by In Silico Studies as Inhibitors of SARS-CoV-2 Cell Entry

    doi: 10.3390/molecules28207204

    Figure Lengend Snippet: Summary of the results obtained for compounds 1 , 2 , 4 , 7 , and 9 .

    Article Snippet: Cell pellets were collected after 48 h incubation, and the following lysis, quantification, and Western Blot procedures were performed as previously described [ ], except for the following points: (i) no phosphatase inhibitor was used; (ii) a total of 15 μg of protein lysates obtained from MDA-MB-231 cells treatment or 50 μg of protein lysates obtained from Vero CCL-81 cells were loaded and separated in 10% SDS-Page gels; (iii) the transference to a nitrocellulose membrane (GE Healthcare Life science, Chalfont St Giles, UK; GE10600002) occurred for 1 h 45 min at 100 V; (iv) the primary antibodies (1:2000) used were: mouse anti-ACE2 monoclonal antibody (CL4035; Thermo Fischer Scientific; Waltham, MA, USA) and mouse anti-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: