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  • 81
    Agilent technologies mouse microarrays
    Mouse Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 81/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse agilent
    Mouse Agilent, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 22k mouse microarrays
    22k Mouse Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies a mouse microarray platform
    A Mouse Microarray Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse microrna microarray
    Mouse Microrna Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse 44k oligoarray
    Mouse 44k Oligoarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies whole mouse microarrays
    Whole Mouse Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse oligo microarray
    Mouse Oligo Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse microarray slide
    Mouse Microarray Slide, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse genomic probes
    Mouse Genomic Probes, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse cgi array
    Mouse Cgi Array, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mus musculus whole mouse genome array
    Mus Musculus Whole Mouse Genome Array, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse mirna microarray
    Mouse Mirna Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse anti ki67
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    Mouse Anti Ki67, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse development oligo microarray
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    Mouse Development Oligo Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse proximal promoter microarrays
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    Mouse Proximal Promoter Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies nia mouse 44k microarray
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    Nia Mouse 44k Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 1m mouse promoter microarrays
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    1m Mouse Promoter Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse expression arrays
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    Mouse Expression Arrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse ge 4x44k microarray
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    Mouse Ge 4x44k Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 4×44k mouse microarrays
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    4×44k Mouse Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse oligonucleotide microarrays
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    Mouse Oligonucleotide Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse mirna microarrays v2
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    Mouse Mirna Microarrays V2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 44k mouse development oligo microarrays
    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: <t>KI67</t> . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).
    44k Mouse Development Oligo Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mouse 60 mer oligo microarray
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    Genetically labeled HSCs obtain a new “inactivated” phenotype after 1 mo of recovery. ( A ) <t>Microarray</t> analysis. Vitamin A + HSCs were sort-purified from Col-α2(I) Cre-YFP mice that were untreated ( n = 6), fibrotic ( n = 6), after 7 d of recovery ( n = 3), and after 1 mo of recovery ( n = 6). YFP + and YFP − HSCs were then subjected to the Whole Mouse Genome Microarray. Representative cell number is shown for each HSC group. ( B ) YFP + iHSCs (1 mo recovery) down-regulate mRNAs of fibrogenic genes and up-regulate PPARγ and Bambi but not other qHSC genes (Adfp, Adipor1, GFAP). The results show the relative mRNA level (average of normalized values/multiple probes/gene) obtained using the Agilant microarray; * P
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    Genetically labeled HSCs obtain a new “inactivated” phenotype after 1 mo of recovery. ( A ) <t>Microarray</t> analysis. Vitamin A + HSCs were sort-purified from Col-α2(I) Cre-YFP mice that were untreated ( n = 6), fibrotic ( n = 6), after 7 d of recovery ( n = 3), and after 1 mo of recovery ( n = 6). YFP + and YFP − HSCs were then subjected to the Whole Mouse Genome Microarray. Representative cell number is shown for each HSC group. ( B ) YFP + iHSCs (1 mo recovery) down-regulate mRNAs of fibrogenic genes and up-regulate PPARγ and Bambi but not other qHSC genes (Adfp, Adipor1, GFAP). The results show the relative mRNA level (average of normalized values/multiple probes/gene) obtained using the Agilant microarray; * P
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    Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: KI67 . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).

    Journal: PLoS ONE

    Article Title: Expansion of Multipotent Stem Cells from the Adult Human Brain

    doi: 10.1371/journal.pone.0071334

    Figure Lengend Snippet: Characteristics of human brain stem cells. A. mRNA Phenotype of four groups of brain derived stem cells. mRNA levels in stem cell cultures (mean of three humans) comparing those derived from Subventricular Zone (SVZ), Hippocampus (HPC), White Matter (WM) and Grey Matter (GM). These are some common genes some of which were already assessed using immunohistochemical techniques as we have described above. Data are from the same microarray platform and were processed together. Ordinate axis units are mean probe strength (Log 2 ) recorded for the genes specified on the co-ordinate axis. Microarray data were ‘quantile normalised’. ▸ : Basal level of transcription. Markers: Housekeeping: Prolyl-4-hydroxylase, β-actin, γ-actin, GAPDH . Dividing cells: KI67 . Stem cells: OCT 4 , SOX2 , EGF receptor, integrin-β1, nestin. Neurons: β-tubulin3, MAP2 , neurofilament. Glia: GFAP, S100 . Oligodendrocytes: OMG , claudin11, GALC . Smooth muscle: α-actin smooth. Skeletal muscle: α-actin skeletal. Cardiac muscle: α-actin cardiac, cardiac troponin I. Dopaminergic cells: Tyrosine hydroxylase, dopamine transporter. B. Western blot confirmed robust expression of stem cell ‘pluripotency’ marker OCT 4 (POU5f1). 40 µg total protein was run on each lane. Lanes 1 and 2: normal samples: 1, HPC, 2, SVZ; Lanes 3–9: tumor stem cells. C. Cells (grown adherently) and then subsequently plated in neurosphere-forming conditions. D. From Table in D, it can be seen that though the sphere generating cells from the different sources varied in density, the cells generated from them had similar growth ability (number of cells per sphere).

    Article Snippet: Antibodies used for Immunohistochemical Labeling Rabbit anti-Gal C, 1/200 (Sigma); rabbit anti-GFAP, 1/200 (DakoCytomation, Denmark); rabbit anti-Integrin β1, 1/200 (Abcam); rabbit anti-Musashi, 1/200 (Abcam); rabbit anti-Dopamine transporter, 1/200 (Sigma); goat anti-Oct 4, 1/200 (R & D Systems); goat anti-human Sox2, 1/200 (R & D Systems); mouse anti-Tyrosine Hydroxylase, 1/50 (Sigma); mouse anti-O4, 1/400 (Millipore); mouse anti-S100, 1/200 (Abcam); mouse anti-Map2, 1/200 (Millipore); mouse anti-Neurofilament 200, 1/200 (Sigma); mouse anti-Nestin, 1/200 (Millipore); mouse anti-Prolyl-4-hydroxylase, 1/200 (BioSite, Sweden); mouse anti-Ki67, 1/200 (DakoCytomation, Denmark); mouse anti-EGFr (Epidermal growth factor receptor), 1/200 (Abcam); mouse anti-sarcomeric αActin, 1/200 (Sigma); mouse anti-cardiac Troponin I, 1/200 (Millipore); rabbit anti-smooth αActin, 1/200; and goat anti-Green Fluorescent Protein (GFP), 1/300 (Santa Cruz).

    Techniques: Derivative Assay, Immunohistochemistry, Microarray, Western Blot, Expressing, Marker, Generated

    Proliferation and phenotype of brain stem cells. A. Average doubling time was 6.5 days. 50 doublings is > 10 14 . This is a yield of 10 18 from a small biopsy. There are 10 14 cells in a human. B. Morphology of brain-derived stem cells growing adherently. C and D. Cultures derived from Hippocampus appeared identical to those from Subventricular zone. (Bars: +SD, n = 3). Markers of: +ve control, Prol4OHase; dividing cells, Ki67; stem cells, Oct 4, Sox2, Intβ1, EGFr, Mus, Nestin; glia, GFAP, S100; neurons, βTub3, Map2, NF; oligodendrocytes, O4, GalC; dopaminergic cells, TH, DT. E. Cultures maintained phenotype through many passages. (Bars: +SD, n = 3). F. Stratification of cultures (Grey Matter p9). Cultures could be arbitrarily divided into zones based on the appearance of the cells in these zones. Immunophenotype confirmed that the differences in shape also reflected differences in phenotype and suggest that this stratification in some way reflects the dynamics involved in tissue organization (See also Figure S1 . Phenotype of brain stem cell cultures ).

    Journal: PLoS ONE

    Article Title: Expansion of Multipotent Stem Cells from the Adult Human Brain

    doi: 10.1371/journal.pone.0071334

    Figure Lengend Snippet: Proliferation and phenotype of brain stem cells. A. Average doubling time was 6.5 days. 50 doublings is > 10 14 . This is a yield of 10 18 from a small biopsy. There are 10 14 cells in a human. B. Morphology of brain-derived stem cells growing adherently. C and D. Cultures derived from Hippocampus appeared identical to those from Subventricular zone. (Bars: +SD, n = 3). Markers of: +ve control, Prol4OHase; dividing cells, Ki67; stem cells, Oct 4, Sox2, Intβ1, EGFr, Mus, Nestin; glia, GFAP, S100; neurons, βTub3, Map2, NF; oligodendrocytes, O4, GalC; dopaminergic cells, TH, DT. E. Cultures maintained phenotype through many passages. (Bars: +SD, n = 3). F. Stratification of cultures (Grey Matter p9). Cultures could be arbitrarily divided into zones based on the appearance of the cells in these zones. Immunophenotype confirmed that the differences in shape also reflected differences in phenotype and suggest that this stratification in some way reflects the dynamics involved in tissue organization (See also Figure S1 . Phenotype of brain stem cell cultures ).

    Article Snippet: Antibodies used for Immunohistochemical Labeling Rabbit anti-Gal C, 1/200 (Sigma); rabbit anti-GFAP, 1/200 (DakoCytomation, Denmark); rabbit anti-Integrin β1, 1/200 (Abcam); rabbit anti-Musashi, 1/200 (Abcam); rabbit anti-Dopamine transporter, 1/200 (Sigma); goat anti-Oct 4, 1/200 (R & D Systems); goat anti-human Sox2, 1/200 (R & D Systems); mouse anti-Tyrosine Hydroxylase, 1/50 (Sigma); mouse anti-O4, 1/400 (Millipore); mouse anti-S100, 1/200 (Abcam); mouse anti-Map2, 1/200 (Millipore); mouse anti-Neurofilament 200, 1/200 (Sigma); mouse anti-Nestin, 1/200 (Millipore); mouse anti-Prolyl-4-hydroxylase, 1/200 (BioSite, Sweden); mouse anti-Ki67, 1/200 (DakoCytomation, Denmark); mouse anti-EGFr (Epidermal growth factor receptor), 1/200 (Abcam); mouse anti-sarcomeric αActin, 1/200 (Sigma); mouse anti-cardiac Troponin I, 1/200 (Millipore); rabbit anti-smooth αActin, 1/200; and goat anti-Green Fluorescent Protein (GFP), 1/300 (Santa Cruz).

    Techniques: Derivative Assay

    Genetically labeled HSCs obtain a new “inactivated” phenotype after 1 mo of recovery. ( A ) Microarray analysis. Vitamin A + HSCs were sort-purified from Col-α2(I) Cre-YFP mice that were untreated ( n = 6), fibrotic ( n = 6), after 7 d of recovery ( n = 3), and after 1 mo of recovery ( n = 6). YFP + and YFP − HSCs were then subjected to the Whole Mouse Genome Microarray. Representative cell number is shown for each HSC group. ( B ) YFP + iHSCs (1 mo recovery) down-regulate mRNAs of fibrogenic genes and up-regulate PPARγ and Bambi but not other qHSC genes (Adfp, Adipor1, GFAP). The results show the relative mRNA level (average of normalized values/multiple probes/gene) obtained using the Agilant microarray; * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Myofibroblasts revert to an inactive phenotype during regression of liver fibrosis

    doi: 10.1073/pnas.1201840109

    Figure Lengend Snippet: Genetically labeled HSCs obtain a new “inactivated” phenotype after 1 mo of recovery. ( A ) Microarray analysis. Vitamin A + HSCs were sort-purified from Col-α2(I) Cre-YFP mice that were untreated ( n = 6), fibrotic ( n = 6), after 7 d of recovery ( n = 3), and after 1 mo of recovery ( n = 6). YFP + and YFP − HSCs were then subjected to the Whole Mouse Genome Microarray. Representative cell number is shown for each HSC group. ( B ) YFP + iHSCs (1 mo recovery) down-regulate mRNAs of fibrogenic genes and up-regulate PPARγ and Bambi but not other qHSC genes (Adfp, Adipor1, GFAP). The results show the relative mRNA level (average of normalized values/multiple probes/gene) obtained using the Agilant microarray; * P

    Article Snippet: The gene expression profile of HSCs was studied using Whole Mouse Genome Microarray (Agilent).

    Techniques: Labeling, Microarray, Purification, Mouse Assay