Article Title: IL-23, but not IL-12, plays a critical role in inflammation-mediated bone disorders
Figure Lengend Snippet: IL-23 plays an essential role in inflammation-mediated inhibition of bone regeneration. ( A ) BMMSCs mixed with β-TCP were implanted into the dorsal surface of WT and nude mice for 8 weeks. A substantial amount of bone was formed in nude mice, as detected by H E staining. n = 4-5 per group. Scale bar, 50 µm. ( B , C ) IL-12p40 expression levels in implants after 7 days of implantation. Representative images ( B ) and quantification ( C ) of immunohistochemical staining of IL-12p40. n = 4-5 per group. Scale bar, 50 µm. ( D ) BMMSCs mixed with β-TCP plus IL-12 and IL-23 or no cytokine were implanted into the dorsal surface of WT and IL-12p40 -/- mice. New bone formation was detected with H E staining. n = 4-5 per group. Scale bar, 50 µm. ( E-G ) Bone histomorphometric measurements among each group, including ( E ) osteoblast number (N.Ob), ( F ) osteoblast number per bone surface (N.Ob/BS), and ( G ) osteocyte number (N.Ot). ( H ) Representative images and quantification of ectopic bone formation in WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4-5 per group. Scale bar, 50 µm. ( I-K ) Bone histomorphometric measurements, including ( I ) N.Ob, ( J ) N.Ob/BS, and ( K ) N.Ot. ( L ) Osteocalcin (OCN) expression was increased in IL-12p40 -/- mice. Representative images and quantification of immunohistochemical staining of OCN were shown in WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4-5 per group. Scale bar, 50 µm. B, bone; CT, connective tissue; WT, wild-type. Results are shown as mean ± S.D. * p
Article Snippet: To explore the effects of IL-12 and IL-23 on RANKL-induced osteoclastogenesis, we incubated RANKL-treated RAW264.7 cells with IL-12, IL-23, IFN-γ, or IL-17.
Techniques: Inhibition, Mouse Assay, Staining, Expressing, Immunohistochemistry