mouse il-23 Search Results


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  • 94
    Thermo Fisher recombinant mouse il 23
    Recombinant Mouse Il 23, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mouse il 23/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant mouse il 23 - by Bioz Stars, 2021-04
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    86
    R&D Systems il 23
    SR3335 modulates human memory T H 17 and T H 17.1 cells. a Naive human CD4 + T cells were differentiated under T H 17 polarizing conditions (anti-CD3 + <t>IL-23+IL-1β)</t> and treated with vehicle (DMSO) or SR3335. IL-17A and IFNγ expression were analyzed from live, CD45RO + cells by flow cytometry on Day 6. Graphs indicate percent IL-17A + cells and frequency of live cells in cultures with compound treatment. b FACS sorted memory cell (CD4 + CD25 − CD45RO + ) subsets from healthy adult donor peripheral blood were stimulated with PMA and ionomycin to determine cytokine production by flow cytometry after 6 days in culture in the presence of vehicle (V, DMSO) or SR3335 (SR, 5μM). Graphs depict percentage cytokine expression (IL-17A, IL-13, and IFNγ) in the cultures for each subset/sample. FACS analysis of T H 17 and T H 17.1 sorted memory cells to determine c IL-17A and IL-22 expression and d TNFα expression. e Graphs summarizing the percentage TNFα expression in sorted T H 1 and T H 2 memory cells treated with vehicle (V, DMSO) or SR3335 (SR, 5 μM) for 6 days from four different donors. f Nanostring data demonstrating the SR3335-mediated changes in gene expression in T H 17 and T H 17.1 cultures on day 6 from the combined data of three donors described in panel e . Data are presented as mean values ± s.e.m. Student’s two-tailed t tests were performed for statistical analysis. Source data are provided as a Source Data file.
    Il 23, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 23/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 23 - by Bioz Stars, 2021-04
    86/100 stars
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    98
    R&D Systems recombinant mouse il 23
    SR3335 modulates human memory T H 17 and T H 17.1 cells. a Naive human CD4 + T cells were differentiated under T H 17 polarizing conditions (anti-CD3 + <t>IL-23+IL-1β)</t> and treated with vehicle (DMSO) or SR3335. IL-17A and IFNγ expression were analyzed from live, CD45RO + cells by flow cytometry on Day 6. Graphs indicate percent IL-17A + cells and frequency of live cells in cultures with compound treatment. b FACS sorted memory cell (CD4 + CD25 − CD45RO + ) subsets from healthy adult donor peripheral blood were stimulated with PMA and ionomycin to determine cytokine production by flow cytometry after 6 days in culture in the presence of vehicle (V, DMSO) or SR3335 (SR, 5μM). Graphs depict percentage cytokine expression (IL-17A, IL-13, and IFNγ) in the cultures for each subset/sample. FACS analysis of T H 17 and T H 17.1 sorted memory cells to determine c IL-17A and IL-22 expression and d TNFα expression. e Graphs summarizing the percentage TNFα expression in sorted T H 1 and T H 2 memory cells treated with vehicle (V, DMSO) or SR3335 (SR, 5 μM) for 6 days from four different donors. f Nanostring data demonstrating the SR3335-mediated changes in gene expression in T H 17 and T H 17.1 cultures on day 6 from the combined data of three donors described in panel e . Data are presented as mean values ± s.e.m. Student’s two-tailed t tests were performed for statistical analysis. Source data are provided as a Source Data file.
    Recombinant Mouse Il 23, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mouse il 23/product/R&D Systems
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant mouse il 23 - by Bioz Stars, 2021-04
    98/100 stars
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    97
    PeproTech il 23
    <t>IL-23</t> plays a critical role in aging-mediated bone loss. ( A ) Micro-CT images of the trabecular bone in the distal femoral metaphysis of 12-month-old male WT mice and age-sex matched IL-12p35 -/- and IL-12p40 -/- mice. n = 4 per group. Scale bar, 1mm (upper and middle panel), 100 µm (lower panel). ( B ) Micro-CT measurements for the indicated parameters in distal femurs. Bone mineral density (BMD), bone volume/tissue volume (BV/TV), trabecular numbers (Tb.N), and trabecular thickness (Tb.Th) were determined by micro-CT analysis. n = 4 per group. ( C ) The H E staining of femur sections from 12-month-old WT, IL-12p35 -/- , and IL-12p40 -/- mice were shown. n = 4 per group. Scale bar, 50 µm. ( D ) Trabecular percentage (%) was quantified via H E images from the groups described in C . n = 4 per group. ( E , F ) The immunohistochemical analysis ( E ) and quantification ( F ) of ALP expression in the distal femoral metaphysis. n = 4 per group. Scale bar, 50 µm. ( G , H ) The immunohistochemical analysis ( G ) and quantification ( H ) of TRAP expression in the distal femoral metaphysis. n = 4 per group. Scale bar, 50 µm. ALP, alkaline phosphatase; TRAP, tartrate-resistant acid phosphatase; WT, wild-type. Results are shown as mean ± S.D. * p
    Il 23, supplied by PeproTech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 23/product/PeproTech
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 23 - by Bioz Stars, 2021-04
    97/100 stars
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    N/A
    Category Kits ELISA Kits Mouse IL 23 Interleukin 23 ELISA Kit Size 96T Price 609 Reactivity Mouse Uniprot Id Q9EQ14
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    N/A
    Category Protein Products Recombinant Proteins Recombinant Mouse Interleukin 23 IL 23 Protein Size 10μg Price 214 Synonyms SGRF IL 23p19 CLMF p40 IL 12 subunit p40 NKSF2 Purity 95 as
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    Image Search Results


    SR3335 modulates human memory T H 17 and T H 17.1 cells. a Naive human CD4 + T cells were differentiated under T H 17 polarizing conditions (anti-CD3 + IL-23+IL-1β) and treated with vehicle (DMSO) or SR3335. IL-17A and IFNγ expression were analyzed from live, CD45RO + cells by flow cytometry on Day 6. Graphs indicate percent IL-17A + cells and frequency of live cells in cultures with compound treatment. b FACS sorted memory cell (CD4 + CD25 − CD45RO + ) subsets from healthy adult donor peripheral blood were stimulated with PMA and ionomycin to determine cytokine production by flow cytometry after 6 days in culture in the presence of vehicle (V, DMSO) or SR3335 (SR, 5μM). Graphs depict percentage cytokine expression (IL-17A, IL-13, and IFNγ) in the cultures for each subset/sample. FACS analysis of T H 17 and T H 17.1 sorted memory cells to determine c IL-17A and IL-22 expression and d TNFα expression. e Graphs summarizing the percentage TNFα expression in sorted T H 1 and T H 2 memory cells treated with vehicle (V, DMSO) or SR3335 (SR, 5 μM) for 6 days from four different donors. f Nanostring data demonstrating the SR3335-mediated changes in gene expression in T H 17 and T H 17.1 cultures on day 6 from the combined data of three donors described in panel e . Data are presented as mean values ± s.e.m. Student’s two-tailed t tests were performed for statistical analysis. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Genetic and pharmacological inhibition of the nuclear receptor RORα regulates TH17 driven inflammatory disorders

    doi: 10.1038/s41467-020-20385-9

    Figure Lengend Snippet: SR3335 modulates human memory T H 17 and T H 17.1 cells. a Naive human CD4 + T cells were differentiated under T H 17 polarizing conditions (anti-CD3 + IL-23+IL-1β) and treated with vehicle (DMSO) or SR3335. IL-17A and IFNγ expression were analyzed from live, CD45RO + cells by flow cytometry on Day 6. Graphs indicate percent IL-17A + cells and frequency of live cells in cultures with compound treatment. b FACS sorted memory cell (CD4 + CD25 − CD45RO + ) subsets from healthy adult donor peripheral blood were stimulated with PMA and ionomycin to determine cytokine production by flow cytometry after 6 days in culture in the presence of vehicle (V, DMSO) or SR3335 (SR, 5μM). Graphs depict percentage cytokine expression (IL-17A, IL-13, and IFNγ) in the cultures for each subset/sample. FACS analysis of T H 17 and T H 17.1 sorted memory cells to determine c IL-17A and IL-22 expression and d TNFα expression. e Graphs summarizing the percentage TNFα expression in sorted T H 1 and T H 2 memory cells treated with vehicle (V, DMSO) or SR3335 (SR, 5 μM) for 6 days from four different donors. f Nanostring data demonstrating the SR3335-mediated changes in gene expression in T H 17 and T H 17.1 cultures on day 6 from the combined data of three donors described in panel e . Data are presented as mean values ± s.e.m. Student’s two-tailed t tests were performed for statistical analysis. Source data are provided as a Source Data file.

    Article Snippet: Other cytokines used for various TH 17 conditions: IL-1β (10 ng/ml, R & D Systems) and IL-23 (20 ng/ml, R & D Systems).

    Techniques: Expressing, Flow Cytometry, FACS, Two Tailed Test

    IL-23 plays a critical role in aging-mediated bone loss. ( A ) Micro-CT images of the trabecular bone in the distal femoral metaphysis of 12-month-old male WT mice and age-sex matched IL-12p35 -/- and IL-12p40 -/- mice. n = 4 per group. Scale bar, 1mm (upper and middle panel), 100 µm (lower panel). ( B ) Micro-CT measurements for the indicated parameters in distal femurs. Bone mineral density (BMD), bone volume/tissue volume (BV/TV), trabecular numbers (Tb.N), and trabecular thickness (Tb.Th) were determined by micro-CT analysis. n = 4 per group. ( C ) The H E staining of femur sections from 12-month-old WT, IL-12p35 -/- , and IL-12p40 -/- mice were shown. n = 4 per group. Scale bar, 50 µm. ( D ) Trabecular percentage (%) was quantified via H E images from the groups described in C . n = 4 per group. ( E , F ) The immunohistochemical analysis ( E ) and quantification ( F ) of ALP expression in the distal femoral metaphysis. n = 4 per group. Scale bar, 50 µm. ( G , H ) The immunohistochemical analysis ( G ) and quantification ( H ) of TRAP expression in the distal femoral metaphysis. n = 4 per group. Scale bar, 50 µm. ALP, alkaline phosphatase; TRAP, tartrate-resistant acid phosphatase; WT, wild-type. Results are shown as mean ± S.D. * p

    Journal: Theranostics

    Article Title: IL-23, but not IL-12, plays a critical role in inflammation-mediated bone disorders

    doi: 10.7150/thno.41378

    Figure Lengend Snippet: IL-23 plays a critical role in aging-mediated bone loss. ( A ) Micro-CT images of the trabecular bone in the distal femoral metaphysis of 12-month-old male WT mice and age-sex matched IL-12p35 -/- and IL-12p40 -/- mice. n = 4 per group. Scale bar, 1mm (upper and middle panel), 100 µm (lower panel). ( B ) Micro-CT measurements for the indicated parameters in distal femurs. Bone mineral density (BMD), bone volume/tissue volume (BV/TV), trabecular numbers (Tb.N), and trabecular thickness (Tb.Th) were determined by micro-CT analysis. n = 4 per group. ( C ) The H E staining of femur sections from 12-month-old WT, IL-12p35 -/- , and IL-12p40 -/- mice were shown. n = 4 per group. Scale bar, 50 µm. ( D ) Trabecular percentage (%) was quantified via H E images from the groups described in C . n = 4 per group. ( E , F ) The immunohistochemical analysis ( E ) and quantification ( F ) of ALP expression in the distal femoral metaphysis. n = 4 per group. Scale bar, 50 µm. ( G , H ) The immunohistochemical analysis ( G ) and quantification ( H ) of TRAP expression in the distal femoral metaphysis. n = 4 per group. Scale bar, 50 µm. ALP, alkaline phosphatase; TRAP, tartrate-resistant acid phosphatase; WT, wild-type. Results are shown as mean ± S.D. * p

    Article Snippet: To explore the effects of IL-12 and IL-23 on RANKL-induced osteoclastogenesis, we incubated RANKL-treated RAW264.7 cells with IL-12, IL-23, IFN-γ, or IL-17.

    Techniques: Micro-CT, Mouse Assay, Staining, Immunohistochemistry, Expressing

    IL-23 deficiency improves calvarial defect repair and enhances bone mass. ( A ) Microcomputed tomography (micro-CT) three-dimensional reconstruction of bone repair in mouse calvarial defects. Red dotted lines indicate the periphery of the defect site. n = 4-5 per group. Scale bar, 500 µm (upper panel), 1 mm (lower panel). ( B ) Defect area coverage (%) was calculated via micro-CT images from WT, IL-12p35 -/- , and IL-12p40 -/- mice. ( C-E ) Micro-CT analysis including ( C ) bone volume (BV), ( D ) bone volume/tissue volume (BV/TV), and ( E ) healing score for the extent of bony bridging and bone union. ( F ) The H E staining of calvarial bone defects. n = 4-5 per group. Scale bar, 100 µm. ( G ) Representative images showed trabecular architecture by micro-CT three-dimensional reconstruction in distal femurs of 2-month-old mice. n = 4-5 per group. Scale bar, 1mm (upper and middle panel), 500 µm (lower panel). ( H ) Micro-CT measurements for the indicated parameters in distal femurs. Bone mineral density (BMD), BV/TV, trabecular numbers (Tb.N), and trabecular thickness (Tb.Th) were determined by micro-CT analysis. n = 4-5 per group. ( I ) The H E staining of femur sections from 2-month-old WT, IL-12p35 -/- , and IL-12p40 -/- mice were shown. n = 4-5 per group. Scale bar, 50 µm. ( J ) Trabecular percentage (%) was quantified via H E images from the groups described in I . WT, wild-type. Results are shown as mean ± S.D. * p

    Journal: Theranostics

    Article Title: IL-23, but not IL-12, plays a critical role in inflammation-mediated bone disorders

    doi: 10.7150/thno.41378

    Figure Lengend Snippet: IL-23 deficiency improves calvarial defect repair and enhances bone mass. ( A ) Microcomputed tomography (micro-CT) three-dimensional reconstruction of bone repair in mouse calvarial defects. Red dotted lines indicate the periphery of the defect site. n = 4-5 per group. Scale bar, 500 µm (upper panel), 1 mm (lower panel). ( B ) Defect area coverage (%) was calculated via micro-CT images from WT, IL-12p35 -/- , and IL-12p40 -/- mice. ( C-E ) Micro-CT analysis including ( C ) bone volume (BV), ( D ) bone volume/tissue volume (BV/TV), and ( E ) healing score for the extent of bony bridging and bone union. ( F ) The H E staining of calvarial bone defects. n = 4-5 per group. Scale bar, 100 µm. ( G ) Representative images showed trabecular architecture by micro-CT three-dimensional reconstruction in distal femurs of 2-month-old mice. n = 4-5 per group. Scale bar, 1mm (upper and middle panel), 500 µm (lower panel). ( H ) Micro-CT measurements for the indicated parameters in distal femurs. Bone mineral density (BMD), BV/TV, trabecular numbers (Tb.N), and trabecular thickness (Tb.Th) were determined by micro-CT analysis. n = 4-5 per group. ( I ) The H E staining of femur sections from 2-month-old WT, IL-12p35 -/- , and IL-12p40 -/- mice were shown. n = 4-5 per group. Scale bar, 50 µm. ( J ) Trabecular percentage (%) was quantified via H E images from the groups described in I . WT, wild-type. Results are shown as mean ± S.D. * p

    Article Snippet: To explore the effects of IL-12 and IL-23 on RANKL-induced osteoclastogenesis, we incubated RANKL-treated RAW264.7 cells with IL-12, IL-23, IFN-γ, or IL-17.

    Techniques: Micro-CT, Mouse Assay, Staining

    IL-12 and IL-23 affect bone formation and resorption in vivo . ( A ) The expression levels of osteoblast-related genes ( ALP and OCN ) in cranium from WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 8 per group. ( B ) Serum levels of OCN. n = 8 per group. ( C ) ALP staining of femur sections from WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4-5 per group. Scale bar, 20 µm. ( D ) Quantification of ALP activity was shown via images from the groups described in C . ( E ) The expression levels of osteoclast-specific genes ( TRAP and RANKL ) in calvarial bone from WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 6 per group. ( F ) Serum levels of CTX-1. n = 6 per group. ( G ) TRAP staining of femur sections from WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4 - 5 per group. Scale bar, 20 µm. ( H ) Quantification of TRAP activity was shown via images from the groups described in G . ALP, alkaline phosphatase; CTX-1, collagen type I cross-linked C-telopeptide; OCN, osteocalcin; RANKL, receptor activator of NF-κB ligand; TRAP, tartrate-resistant acid phosphatase; WT, wild-type. Results are shown as mean ± S.D. * p

    Journal: Theranostics

    Article Title: IL-23, but not IL-12, plays a critical role in inflammation-mediated bone disorders

    doi: 10.7150/thno.41378

    Figure Lengend Snippet: IL-12 and IL-23 affect bone formation and resorption in vivo . ( A ) The expression levels of osteoblast-related genes ( ALP and OCN ) in cranium from WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 8 per group. ( B ) Serum levels of OCN. n = 8 per group. ( C ) ALP staining of femur sections from WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4-5 per group. Scale bar, 20 µm. ( D ) Quantification of ALP activity was shown via images from the groups described in C . ( E ) The expression levels of osteoclast-specific genes ( TRAP and RANKL ) in calvarial bone from WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 6 per group. ( F ) Serum levels of CTX-1. n = 6 per group. ( G ) TRAP staining of femur sections from WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4 - 5 per group. Scale bar, 20 µm. ( H ) Quantification of TRAP activity was shown via images from the groups described in G . ALP, alkaline phosphatase; CTX-1, collagen type I cross-linked C-telopeptide; OCN, osteocalcin; RANKL, receptor activator of NF-κB ligand; TRAP, tartrate-resistant acid phosphatase; WT, wild-type. Results are shown as mean ± S.D. * p

    Article Snippet: To explore the effects of IL-12 and IL-23 on RANKL-induced osteoclastogenesis, we incubated RANKL-treated RAW264.7 cells with IL-12, IL-23, IFN-γ, or IL-17.

    Techniques: In Vivo, Expressing, Mouse Assay, Staining, Activity Assay

    IL-12 and IL-23 regulate BMMSC osteogenesis and osteoclast differentiation. ( A , B ) BMMSCs mixed with β-TCP plus IL-12 or IL-12+IL-23 were subcutaneously implanted into immunocompromised mice for 8 weeks. ( A ) New bone formation was detected with H E staining. n = 4-5 per group. Scale bar, 50 µm. ( B ) Quantitative analysis of new bone volume. n = 4-5 per group. ( C-E ) Bone histomorphometric measurements among each group, including ( C ) osteoblast number (N.Ob), ( D ) osteoblast number per bone surface (N.Ob/BS), and ( E ) osteocyte number (N.Ot). ( F ) Compared to untreated CD4 + T cell culture supernatants, IL-12- or/and IL-23-stimulated supernatants showed reduced capacities to form mineralized nodules of BMMSCs, as assessed by Alizarin Red staining. ( G ) Quantitative analysis of the calcium mineralization in F . ( H ) The blockade of IFN-γ or/and IL-17 in IL-12- and IL-23-stimulated supernatants rescued reduced capacities to form mineralized nodules in BMMSCs. ( I ) Quantitative analysis of the calcium mineralization in H . ( J ) RAW264.7 cell proliferation analysis treated with RANKL (75 ng/mL) and different cytokines. All: IL-12 + IL-23 + IFN-γ + IL-17. *: compared with control group; #: compared with IL-12 + IL-23 group; x: compared with All group. ( K ) Effects of different cytokines (IL-12, IL-23, IFN-γ, and IL-17) on the RANKL-induced osteoclast differentiation of RAW264.7 cells. ( L, M ) The number of TRAP + osteoclasts per field ( L ) and the average size of osteoclasts ( M ). All: IL-12 + IL-23 + IFN-γ + IL-17. *: compared with control group; #: compared with IL-12 + IL-23 group; x: compared with All group. B, bone; CT, connective tissue; Con, control; CM, control medium; IFN-γ, interferon γ; OM, osteogenic medium; RANKL, receptor activator of NF-κB ligand. Results are shown as mean ± S.D. * p

    Journal: Theranostics

    Article Title: IL-23, but not IL-12, plays a critical role in inflammation-mediated bone disorders

    doi: 10.7150/thno.41378

    Figure Lengend Snippet: IL-12 and IL-23 regulate BMMSC osteogenesis and osteoclast differentiation. ( A , B ) BMMSCs mixed with β-TCP plus IL-12 or IL-12+IL-23 were subcutaneously implanted into immunocompromised mice for 8 weeks. ( A ) New bone formation was detected with H E staining. n = 4-5 per group. Scale bar, 50 µm. ( B ) Quantitative analysis of new bone volume. n = 4-5 per group. ( C-E ) Bone histomorphometric measurements among each group, including ( C ) osteoblast number (N.Ob), ( D ) osteoblast number per bone surface (N.Ob/BS), and ( E ) osteocyte number (N.Ot). ( F ) Compared to untreated CD4 + T cell culture supernatants, IL-12- or/and IL-23-stimulated supernatants showed reduced capacities to form mineralized nodules of BMMSCs, as assessed by Alizarin Red staining. ( G ) Quantitative analysis of the calcium mineralization in F . ( H ) The blockade of IFN-γ or/and IL-17 in IL-12- and IL-23-stimulated supernatants rescued reduced capacities to form mineralized nodules in BMMSCs. ( I ) Quantitative analysis of the calcium mineralization in H . ( J ) RAW264.7 cell proliferation analysis treated with RANKL (75 ng/mL) and different cytokines. All: IL-12 + IL-23 + IFN-γ + IL-17. *: compared with control group; #: compared with IL-12 + IL-23 group; x: compared with All group. ( K ) Effects of different cytokines (IL-12, IL-23, IFN-γ, and IL-17) on the RANKL-induced osteoclast differentiation of RAW264.7 cells. ( L, M ) The number of TRAP + osteoclasts per field ( L ) and the average size of osteoclasts ( M ). All: IL-12 + IL-23 + IFN-γ + IL-17. *: compared with control group; #: compared with IL-12 + IL-23 group; x: compared with All group. B, bone; CT, connective tissue; Con, control; CM, control medium; IFN-γ, interferon γ; OM, osteogenic medium; RANKL, receptor activator of NF-κB ligand. Results are shown as mean ± S.D. * p

    Article Snippet: To explore the effects of IL-12 and IL-23 on RANKL-induced osteoclastogenesis, we incubated RANKL-treated RAW264.7 cells with IL-12, IL-23, IFN-γ, or IL-17.

    Techniques: Mouse Assay, Staining, Cell Culture

    IL-23 plays an essential role in inflammation-mediated inhibition of bone regeneration. ( A ) BMMSCs mixed with β-TCP were implanted into the dorsal surface of WT and nude mice for 8 weeks. A substantial amount of bone was formed in nude mice, as detected by H E staining. n = 4-5 per group. Scale bar, 50 µm. ( B , C ) IL-12p40 expression levels in implants after 7 days of implantation. Representative images ( B ) and quantification ( C ) of immunohistochemical staining of IL-12p40. n = 4-5 per group. Scale bar, 50 µm. ( D ) BMMSCs mixed with β-TCP plus IL-12 and IL-23 or no cytokine were implanted into the dorsal surface of WT and IL-12p40 -/- mice. New bone formation was detected with H E staining. n = 4-5 per group. Scale bar, 50 µm. ( E-G ) Bone histomorphometric measurements among each group, including ( E ) osteoblast number (N.Ob), ( F ) osteoblast number per bone surface (N.Ob/BS), and ( G ) osteocyte number (N.Ot). ( H ) Representative images and quantification of ectopic bone formation in WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4-5 per group. Scale bar, 50 µm. ( I-K ) Bone histomorphometric measurements, including ( I ) N.Ob, ( J ) N.Ob/BS, and ( K ) N.Ot. ( L ) Osteocalcin (OCN) expression was increased in IL-12p40 -/- mice. Representative images and quantification of immunohistochemical staining of OCN were shown in WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4-5 per group. Scale bar, 50 µm. B, bone; CT, connective tissue; WT, wild-type. Results are shown as mean ± S.D. * p

    Journal: Theranostics

    Article Title: IL-23, but not IL-12, plays a critical role in inflammation-mediated bone disorders

    doi: 10.7150/thno.41378

    Figure Lengend Snippet: IL-23 plays an essential role in inflammation-mediated inhibition of bone regeneration. ( A ) BMMSCs mixed with β-TCP were implanted into the dorsal surface of WT and nude mice for 8 weeks. A substantial amount of bone was formed in nude mice, as detected by H E staining. n = 4-5 per group. Scale bar, 50 µm. ( B , C ) IL-12p40 expression levels in implants after 7 days of implantation. Representative images ( B ) and quantification ( C ) of immunohistochemical staining of IL-12p40. n = 4-5 per group. Scale bar, 50 µm. ( D ) BMMSCs mixed with β-TCP plus IL-12 and IL-23 or no cytokine were implanted into the dorsal surface of WT and IL-12p40 -/- mice. New bone formation was detected with H E staining. n = 4-5 per group. Scale bar, 50 µm. ( E-G ) Bone histomorphometric measurements among each group, including ( E ) osteoblast number (N.Ob), ( F ) osteoblast number per bone surface (N.Ob/BS), and ( G ) osteocyte number (N.Ot). ( H ) Representative images and quantification of ectopic bone formation in WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4-5 per group. Scale bar, 50 µm. ( I-K ) Bone histomorphometric measurements, including ( I ) N.Ob, ( J ) N.Ob/BS, and ( K ) N.Ot. ( L ) Osteocalcin (OCN) expression was increased in IL-12p40 -/- mice. Representative images and quantification of immunohistochemical staining of OCN were shown in WT, IL-12p35 -/- , and IL-12p40 -/- mice. n = 4-5 per group. Scale bar, 50 µm. B, bone; CT, connective tissue; WT, wild-type. Results are shown as mean ± S.D. * p

    Article Snippet: To explore the effects of IL-12 and IL-23 on RANKL-induced osteoclastogenesis, we incubated RANKL-treated RAW264.7 cells with IL-12, IL-23, IFN-γ, or IL-17.

    Techniques: Inhibition, Mouse Assay, Staining, Expressing, Immunohistochemistry