mouse igg isotype control Search Results


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  • 99
    Thermo Fisher mouse igg2a isotype control
    T-dependent <t>IgG</t> responses in WT and mutant mice with germline, partial or conditional knockout of Fcgr2b. Mice were immunized with NP-CGG in complete Freund’s adjuvant on day 0 and boosted with NP-CGG in incomplete adjuvant on day 28 (indicated
    Mouse Igg2a Isotype Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    94
    Millipore isotype control mouse igg
    Protective effects of polyclonal anti-dgA <t>IgG</t> and anti-RTA 31RA aptamer on ricin-inhibited luciferase activity. A: Protective effects of anti-dgA IgG against ricin-inhibited luciferase activity, but not <t>anisomycin-inhibited</t> luciferase activity; B: Protective
    Isotype Control Mouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
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    96
    R&D Systems mouse igg2a isotype control
    Transmammary transfer of BMP9 and BMP10 blocking Abs leads to abnormal hypervascularization in neonatal retinas. ( A – C ”) Representative images of fluorescent isolectin B4-stained retinas either from P6 neonates fed for 3 days by dams injected on P3 with PBS ( A ), control <t>IgG2a/b</t> Abs ( B ), or BMP9/10 blocking Abs ( C – C ”). ( C’ , C” ) are higher magnification images of the relevant boxed areas in C. a, artery; v, vein; scale bars, 500 μm. ( D–L ) Higher magnification showing retinal vasculature fields between an artery and a vein (Plexus, D–F ), or at the front of an artery ( G–I ) or a vein ( J–L ) from neonates treated as in ( A–C ). Scale bars, 100 μm. ( M–O ) Scatter plots showing the vascular density on retinal ‘petals’ at the plexus ( M ), artery front ( N ), or vein front ( O ). Data represent mean ± s.e.m. (n = 6 pups per group from 2 dams); **** P
    Mouse Igg2a Isotype Control, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 241 article reviews
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    92
    Novus Biologicals mouse igg1 isotype control
    Transmammary transfer of BMP9 and BMP10 blocking Abs leads to abnormal hypervascularization in neonatal retinas. ( A – C ”) Representative images of fluorescent isolectin B4-stained retinas either from P6 neonates fed for 3 days by dams injected on P3 with PBS ( A ), control <t>IgG2a/b</t> Abs ( B ), or BMP9/10 blocking Abs ( C – C ”). ( C’ , C” ) are higher magnification images of the relevant boxed areas in C. a, artery; v, vein; scale bars, 500 μm. ( D–L ) Higher magnification showing retinal vasculature fields between an artery and a vein (Plexus, D–F ), or at the front of an artery ( G–I ) or a vein ( J–L ) from neonates treated as in ( A–C ). Scale bars, 100 μm. ( M–O ) Scatter plots showing the vascular density on retinal ‘petals’ at the plexus ( M ), artery front ( N ), or vein front ( O ). Data represent mean ± s.e.m. (n = 6 pups per group from 2 dams); **** P
    Mouse Igg1 Isotype Control, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    R&D Systems igg2b isotype control
    AGE-LDL increased cell surface expression of CCR2. Macrophages were incubated in the presence of either LDL or AGE-LDL for 48 hours, and CCR2 surface expression was determined by flow cytometry with anti-CCR2 <t>IgG</t> and expressed as specific mean fluorescence intensity (MFI). Data are shown as mean ± SEM (n=3). The experiments were done twice for each donor.
    Igg2b Isotype Control, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    R&D Systems mouse igg2a isotype controls
    The effect of type I IFNs to SOCS2 mRNA induction in human moDCs. moDCs were exposed to LPS (A) for indicated time periods for mRNA measurement of IFNα, IFNβ, IFNγ and IFNλ1 or incubated with IFNα (Bi), IFNβ (Bii), IFNγ (Biii) and IFNλ1 (Biv) for indicated time periods for SOCS2 mRNA measurement by qRT-PCR. (C) moDCs were pretreated for 30 min with or without 30 ug/ml neutralizing anti-IFNR2 antibodies or <t>IgG</t> 2a isotype control mAb and then incubated for 4 h with or without LPS for SOCS2 mRNA measurement by qRT-PCR. Data shown are representative of three independent experiments and expressed as the –fold induction of the gene of interest at different time points compared to time point 0 where the values were set to 1(A–B) or different treatment conditions compared with medium that values were set to 1. Statistical significance of results with anti-IFNAR2 mAb compared with control mAb is indicated (C) (* p
    Mouse Igg2a Isotype Controls, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher isotype control mouse igg1
    Reactivation of OM-specific T-cell responses by dual blockade of the PD-1 and LAG-3 pathways. PBMCs were isolated from the blood of A. marginale -challenged cattle at 34 dpi and cultured in triplicate for 7 days with 0.2 μg/ml OM antigen and no antibody or 10 μg/ml of one or two blocking antibodies, including anti-PD-L1 MAb, anti-LAG-3 MAb, and control rat <t>IgG,</t> to reach a total concentration of 20 μg/ml IgG in the presence of OM antigen. Medium and uRBC antigen were included as negative controls. (A) PBMC proliferation presented as Δcpm values for each animal after subtracting the mean counts per minute for cells cultured with uRBCs from the mean counts per minute for cells cultured with OMs. (B) Supernatants were harvested from the proliferation assay mixture on day 6 and pooled, and IFN-γ production for each animal was measured by an ELISA in duplicate. Bars indicate group mean responses. Asterisks indicate significant differences between control IgG and anti-LAG-3 MAb plus anti-PD-L1 MAb, where the P value was
    Isotype Control Mouse Igg1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    R&D Systems mouse igg1 isotype control apc
    Role of glycosylation and keratin binding in Srr1-mediated GBS cell attachment. (A) Adherence of GBS strains NCTC10/84 and the Δ srr-1 mutant to VK2/E6E7 vaginal epithelial cells preincubated with anti-cytokeratin peptide 4 antibody (KT4) or an <t>IgG1</t> isotope control. (B) Adherence of GBS strains NCTC10/84 and the Δ srr-1 mutant to VK2/E6E7 vaginal epithelial cells preincubated with wheat germ agglutinin (WGA). All experiments were repeated at least two times in triplicate; data from a representative experiment are shown. Error bars indicate 95% confidence intervals of mean values from three wells. *, P
    Mouse Igg1 Isotype Control Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore mouse igg3
    TE-7 stains cells below the epidermis in normal skin tissue. Human dermis was collected from breast tissue, fixed, and paraffin-embedded. Slides were cut and stained with an <t>IgG1</t> isotype control or the TE-7 antibody as described in Materials and Methods.
    Mouse Igg3, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    T-dependent IgG responses in WT and mutant mice with germline, partial or conditional knockout of Fcgr2b. Mice were immunized with NP-CGG in complete Freund’s adjuvant on day 0 and boosted with NP-CGG in incomplete adjuvant on day 28 (indicated

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Inhibitory Fcγ receptor is required for the maintenance of tolerance through distinct mechanisms

    doi: 10.4049/jimmunol.1302934

    Figure Lengend Snippet: T-dependent IgG responses in WT and mutant mice with germline, partial or conditional knockout of Fcgr2b. Mice were immunized with NP-CGG in complete Freund’s adjuvant on day 0 and boosted with NP-CGG in incomplete adjuvant on day 28 (indicated

    Article Snippet: In order to analyze the levels of FcγRIIB in WT and mutant mice with germline or conditional knockout of Fcgr2b, blood cells and splenic single-cell suspensions were prepared and depleted for erythrocytes, and stained with fluorescent-conjugated anti-CD19 (1D3), anti-NK1.1(PK136), anti-CD11b (M1/70), anti-CD11c (HL3), anti-Gr1 (RB6-8C5), and biotin-conjugated anti-FcγRIIB (Ly17.2) or mouse IgG2a isotype control (MG2a15, Invitrogen) antibodies in the first step and streptavidin-APC in the second step.

    Techniques: Mutagenesis, Mouse Assay, Knock-Out

    Spontaneous anti-nuclear antibodies in WT and mutant mice with germline or conditional knockout of Fcgr2b . (A) Levels of anti-nuclear antibodies of IgG classes in 10 month old WT and mutant mice with germline or conditional knockout of Fcgr2b (16~27 mice

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Inhibitory Fcγ receptor is required for the maintenance of tolerance through distinct mechanisms

    doi: 10.4049/jimmunol.1302934

    Figure Lengend Snippet: Spontaneous anti-nuclear antibodies in WT and mutant mice with germline or conditional knockout of Fcgr2b . (A) Levels of anti-nuclear antibodies of IgG classes in 10 month old WT and mutant mice with germline or conditional knockout of Fcgr2b (16~27 mice

    Article Snippet: In order to analyze the levels of FcγRIIB in WT and mutant mice with germline or conditional knockout of Fcgr2b, blood cells and splenic single-cell suspensions were prepared and depleted for erythrocytes, and stained with fluorescent-conjugated anti-CD19 (1D3), anti-NK1.1(PK136), anti-CD11b (M1/70), anti-CD11c (HL3), anti-Gr1 (RB6-8C5), and biotin-conjugated anti-FcγRIIB (Ly17.2) or mouse IgG2a isotype control (MG2a15, Invitrogen) antibodies in the first step and streptavidin-APC in the second step.

    Techniques: Mutagenesis, Mouse Assay, Knock-Out

    Protective effects of polyclonal anti-dgA IgG and anti-RTA 31RA aptamer on ricin-inhibited luciferase activity. A: Protective effects of anti-dgA IgG against ricin-inhibited luciferase activity, but not anisomycin-inhibited luciferase activity; B: Protective

    Journal:

    Article Title: Protective effects of anti-ricin A-chain RNA aptamer against ricin toxicity

    doi: 10.3748/wjg.14.6360

    Figure Lengend Snippet: Protective effects of polyclonal anti-dgA IgG and anti-RTA 31RA aptamer on ricin-inhibited luciferase activity. A: Protective effects of anti-dgA IgG against ricin-inhibited luciferase activity, but not anisomycin-inhibited luciferase activity; B: Protective

    Article Snippet: Isotype control mouse IgG, anisomycin and doxycycline were obtained from Sigma-Aldrich.

    Techniques: Luciferase, Activity Assay

    Protective effects of polyclonal anti-dgA IgG and anti-RTA 31RA aptamer on ricin-induced cell cytotoxicity as measured by MTS assay. A: Protective effects of anti-dgA IgG against ricin-induced cytotoxicity, but not anisomycin-induced cytotoxicity; B:

    Journal:

    Article Title: Protective effects of anti-ricin A-chain RNA aptamer against ricin toxicity

    doi: 10.3748/wjg.14.6360

    Figure Lengend Snippet: Protective effects of polyclonal anti-dgA IgG and anti-RTA 31RA aptamer on ricin-induced cell cytotoxicity as measured by MTS assay. A: Protective effects of anti-dgA IgG against ricin-induced cytotoxicity, but not anisomycin-induced cytotoxicity; B:

    Article Snippet: Isotype control mouse IgG, anisomycin and doxycycline were obtained from Sigma-Aldrich.

    Techniques: MTS Assay

    UT12 induces activation of NF-κB and production of proinflammatory cytokines in vitro. (A and B) NF-κB reporter Ba/F3 cells expressing mouse (A) or human (B) TLR4/MD-2 were stimulated with LPS, UT12, UT15, or Y5606. (C) Ba/F3/mTLR4f/mMD-2f/κBluc was stimulated with LPS or UT12 with or without serum. (D) Ba/F3/mTLR4f/mMD-2f/κBluc was preincubated for 30 min with 20 μg/ml anti-CD14 antibody or an isotype control with 10% mouse serum, followed by stimulation with LPS or UT12. (E) Ba/F3/mTLR4f/mMD-2f/κBluc was stimulated with heat-treated or nontreated UT12 or LPS. P, PBS. (F) Ba/F3/mTLR4f/mMD-2f/κBluc was stimulated with UT12, repurified UT12 (repurified by protein G) (pro G), or IgG-depleted UT12. The NF-κB activities relative to those of unstimulated cells are plotted. (G) PECs from C3H/HeN or C3H/HeJ mice were plated at 1 × 10 5 cells in 100 μl culture medium containing 5 ng/ml LPS or 100 ng/ml UT12 or Y5606. After 5 h, the supernatant was examined by ELISA for TNF-α and IL-6. The data represent the means ± standard deviations for three wells. L, LPS; U, UT12; Y, Y5606.

    Journal: Clinical and Vaccine Immunology

    Article Title: Induction of Long-Term Lipopolysaccharide Tolerance by an Agonistic Monoclonal Antibody to the Toll-Like Receptor 4/MD-2 Complex

    doi: 10.1128/CVI.00173-06

    Figure Lengend Snippet: UT12 induces activation of NF-κB and production of proinflammatory cytokines in vitro. (A and B) NF-κB reporter Ba/F3 cells expressing mouse (A) or human (B) TLR4/MD-2 were stimulated with LPS, UT12, UT15, or Y5606. (C) Ba/F3/mTLR4f/mMD-2f/κBluc was stimulated with LPS or UT12 with or without serum. (D) Ba/F3/mTLR4f/mMD-2f/κBluc was preincubated for 30 min with 20 μg/ml anti-CD14 antibody or an isotype control with 10% mouse serum, followed by stimulation with LPS or UT12. (E) Ba/F3/mTLR4f/mMD-2f/κBluc was stimulated with heat-treated or nontreated UT12 or LPS. P, PBS. (F) Ba/F3/mTLR4f/mMD-2f/κBluc was stimulated with UT12, repurified UT12 (repurified by protein G) (pro G), or IgG-depleted UT12. The NF-κB activities relative to those of unstimulated cells are plotted. (G) PECs from C3H/HeN or C3H/HeJ mice were plated at 1 × 10 5 cells in 100 μl culture medium containing 5 ng/ml LPS or 100 ng/ml UT12 or Y5606. After 5 h, the supernatant was examined by ELISA for TNF-α and IL-6. The data represent the means ± standard deviations for three wells. L, LPS; U, UT12; Y, Y5606.

    Article Snippet: Y5606, an isotype control IgG3, and anti-FLAG M2 antibody were from Sigma.

    Techniques: Activation Assay, In Vitro, Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Transmammary transfer of BMP9 and BMP10 blocking Abs leads to abnormal hypervascularization in neonatal retinas. ( A – C ”) Representative images of fluorescent isolectin B4-stained retinas either from P6 neonates fed for 3 days by dams injected on P3 with PBS ( A ), control IgG2a/b Abs ( B ), or BMP9/10 blocking Abs ( C – C ”). ( C’ , C” ) are higher magnification images of the relevant boxed areas in C. a, artery; v, vein; scale bars, 500 μm. ( D–L ) Higher magnification showing retinal vasculature fields between an artery and a vein (Plexus, D–F ), or at the front of an artery ( G–I ) or a vein ( J–L ) from neonates treated as in ( A–C ). Scale bars, 100 μm. ( M–O ) Scatter plots showing the vascular density on retinal ‘petals’ at the plexus ( M ), artery front ( N ), or vein front ( O ). Data represent mean ± s.e.m. (n = 6 pups per group from 2 dams); **** P

    Journal: Scientific Reports

    Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

    doi: 10.1038/srep37366

    Figure Lengend Snippet: Transmammary transfer of BMP9 and BMP10 blocking Abs leads to abnormal hypervascularization in neonatal retinas. ( A – C ”) Representative images of fluorescent isolectin B4-stained retinas either from P6 neonates fed for 3 days by dams injected on P3 with PBS ( A ), control IgG2a/b Abs ( B ), or BMP9/10 blocking Abs ( C – C ”). ( C’ , C” ) are higher magnification images of the relevant boxed areas in C. a, artery; v, vein; scale bars, 500 μm. ( D–L ) Higher magnification showing retinal vasculature fields between an artery and a vein (Plexus, D–F ), or at the front of an artery ( G–I ) or a vein ( J–L ) from neonates treated as in ( A–C ). Scale bars, 100 μm. ( M–O ) Scatter plots showing the vascular density on retinal ‘petals’ at the plexus ( M ), artery front ( N ), or vein front ( O ). Data represent mean ± s.e.m. (n = 6 pups per group from 2 dams); **** P

    Article Snippet: Ab injections and transmammary transfer of Abs via lactation Lactating dams were injected i.p. once on P3 with PBS, mouse monoclonal isotype control Abs (15 mg/kg, IgG2b, MAB004; 15 mg/kg, IgG2a, MAB003; R & D Systems), or mouse monoclonal anti-BMP9 and anti-BMP10 Abs (15 mg/kg, IgG2b, MAB3209; 15 mg/kg, IgG2a, MAB2926; R & D Systems, respectively).

    Techniques: Blocking Assay, Staining, Injection

    Transmammary transfer of BMP9 and BMP10 blocking Abs into the circulation of mouse neonates. ( A–D ) ELISAs were performed to measure IgG2a ( A , C ) and anti-BMP9 Ab ( B , D ) levels in the serum of P6 neonates treated at P3 with vehicle (PBS), isotype control IgGs (IgG2a/2b), or anti-BMP9/10 Abs. Neonates were treated during lactation either from dams injected i.p. with ( A , B ), or by direct i.p. injections of ( C , D ), the different Abs or vehicle. Data represent mean ± s.e.m. (n = 5–7 pups per group from 2 dams); **** P

    Journal: Scientific Reports

    Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

    doi: 10.1038/srep37366

    Figure Lengend Snippet: Transmammary transfer of BMP9 and BMP10 blocking Abs into the circulation of mouse neonates. ( A–D ) ELISAs were performed to measure IgG2a ( A , C ) and anti-BMP9 Ab ( B , D ) levels in the serum of P6 neonates treated at P3 with vehicle (PBS), isotype control IgGs (IgG2a/2b), or anti-BMP9/10 Abs. Neonates were treated during lactation either from dams injected i.p. with ( A , B ), or by direct i.p. injections of ( C , D ), the different Abs or vehicle. Data represent mean ± s.e.m. (n = 5–7 pups per group from 2 dams); **** P

    Article Snippet: Ab injections and transmammary transfer of Abs via lactation Lactating dams were injected i.p. once on P3 with PBS, mouse monoclonal isotype control Abs (15 mg/kg, IgG2b, MAB004; 15 mg/kg, IgG2a, MAB003; R & D Systems), or mouse monoclonal anti-BMP9 and anti-BMP10 Abs (15 mg/kg, IgG2b, MAB3209; 15 mg/kg, IgG2a, MAB2926; R & D Systems, respectively).

    Techniques: Blocking Assay, Injection

    Transmammary transfer of BMP9 and BMP10 blocking Abs induces AVMs in neonatal retinas. ( A,B ) Representative images of blue latex-perfused retinal vasculature of P6 neonates fed for 3 days by dams injected on P3 with control IgG2a/b Abs ( A ) or BMP9/10 blocking Abs ( B ). Arrows in B indicate AVMs. Scale bars, 500 μm. ( C ) Scheme depicting the method employed for the quantification of the number of latex dye-positive vessels. ( D ) Histogram showing the total number of vascular crosses. ( E ) Histogram showing the number of vascular crosses per concentric circle on circles 1 to 3. Data represent mean ± s.e.m. (n = 5–7 pups per group from 2 dams); **** P

    Journal: Scientific Reports

    Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

    doi: 10.1038/srep37366

    Figure Lengend Snippet: Transmammary transfer of BMP9 and BMP10 blocking Abs induces AVMs in neonatal retinas. ( A,B ) Representative images of blue latex-perfused retinal vasculature of P6 neonates fed for 3 days by dams injected on P3 with control IgG2a/b Abs ( A ) or BMP9/10 blocking Abs ( B ). Arrows in B indicate AVMs. Scale bars, 500 μm. ( C ) Scheme depicting the method employed for the quantification of the number of latex dye-positive vessels. ( D ) Histogram showing the total number of vascular crosses. ( E ) Histogram showing the number of vascular crosses per concentric circle on circles 1 to 3. Data represent mean ± s.e.m. (n = 5–7 pups per group from 2 dams); **** P

    Article Snippet: Ab injections and transmammary transfer of Abs via lactation Lactating dams were injected i.p. once on P3 with PBS, mouse monoclonal isotype control Abs (15 mg/kg, IgG2b, MAB004; 15 mg/kg, IgG2a, MAB003; R & D Systems), or mouse monoclonal anti-BMP9 and anti-BMP10 Abs (15 mg/kg, IgG2b, MAB3209; 15 mg/kg, IgG2a, MAB2926; R & D Systems, respectively).

    Techniques: Blocking Assay, Injection

    Gene expression changes in BMP9/10-immunoblocked retinas and ALK1-Fc-treated HUVECs. ( A ) RNA-Seq heat map displaying differently expressed genes in mouse whole retinas following transmammary transfer of anti-BMP9/10 or control IgG2a/2b Abs (n = 6 pups per group from 1 dam). ( B ) HUVECs were treated or not (Ctrl) with ALK1-Fc (1 μg/mL, 24 h). Cell extracts were then analyzed by WB using Abs directed against the indicated proteins. ( C ) Densitometric analyses and quantification of phospho-Smad1/5/8, ID1, and ANG2 relative levels in three independent experiments as in ( B ). ( D ) ECs isolated from retinas of pups fed for 3 days by dams injected on P3 with control IgG2a/b Abs (Ctrl) or BMP9/10 blocking Abs (anti-BMP9/10) were analyzed for Id1 and Angpt2 mRNA levels by RT-qPCR. The results are expressed as relative levels of the control condition (n = 3 determinations, n = 6 pups per group from 1 dam). Data in ( C , D ) are mean ± s.e.m.; *** P

    Journal: Scientific Reports

    Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

    doi: 10.1038/srep37366

    Figure Lengend Snippet: Gene expression changes in BMP9/10-immunoblocked retinas and ALK1-Fc-treated HUVECs. ( A ) RNA-Seq heat map displaying differently expressed genes in mouse whole retinas following transmammary transfer of anti-BMP9/10 or control IgG2a/2b Abs (n = 6 pups per group from 1 dam). ( B ) HUVECs were treated or not (Ctrl) with ALK1-Fc (1 μg/mL, 24 h). Cell extracts were then analyzed by WB using Abs directed against the indicated proteins. ( C ) Densitometric analyses and quantification of phospho-Smad1/5/8, ID1, and ANG2 relative levels in three independent experiments as in ( B ). ( D ) ECs isolated from retinas of pups fed for 3 days by dams injected on P3 with control IgG2a/b Abs (Ctrl) or BMP9/10 blocking Abs (anti-BMP9/10) were analyzed for Id1 and Angpt2 mRNA levels by RT-qPCR. The results are expressed as relative levels of the control condition (n = 3 determinations, n = 6 pups per group from 1 dam). Data in ( C , D ) are mean ± s.e.m.; *** P

    Article Snippet: Ab injections and transmammary transfer of Abs via lactation Lactating dams were injected i.p. once on P3 with PBS, mouse monoclonal isotype control Abs (15 mg/kg, IgG2b, MAB004; 15 mg/kg, IgG2a, MAB003; R & D Systems), or mouse monoclonal anti-BMP9 and anti-BMP10 Abs (15 mg/kg, IgG2b, MAB3209; 15 mg/kg, IgG2a, MAB2926; R & D Systems, respectively).

    Techniques: Expressing, RNA Sequencing Assay, Western Blot, Isolation, Injection, Blocking Assay, Quantitative RT-PCR

    Phototoxicity of Pab-IR700. (A) Microscopic observation of 3T3 and 3T3-MDR1 cells treated with Pab-IR700 followed by NIR irradiation. Scale bar, 50 μm. (B) Dose-dependent phototoxicity of Pab-IR700 and IgG-IR700 in 3T3 and 3T3-MDR1 cells after

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: P-glycoprotein Targeted and Near-infrared Light-guided Depletion of Chemoresistant Tumors

    doi: 10.1016/j.jconrel.2018.08.005

    Figure Lengend Snippet: Phototoxicity of Pab-IR700. (A) Microscopic observation of 3T3 and 3T3-MDR1 cells treated with Pab-IR700 followed by NIR irradiation. Scale bar, 50 μm. (B) Dose-dependent phototoxicity of Pab-IR700 and IgG-IR700 in 3T3 and 3T3-MDR1 cells after

    Article Snippet: Mouse IgG1 isotype control (IgG) was purchased from R & D Systems, Inc. (Minneapolis, MN, USA).

    Techniques: Irradiation

    Penetration and phototoxicity of antibody conjugates in tumor spheroids. (A) CLSM images of NCI-ADR Res spheroids after incubation with Pab-640R and IgG-640R overnight. Scale bar, 100 μm. (B) Quantitative distribution of Pab-640R and IgG-640R in

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: P-glycoprotein Targeted and Near-infrared Light-guided Depletion of Chemoresistant Tumors

    doi: 10.1016/j.jconrel.2018.08.005

    Figure Lengend Snippet: Penetration and phototoxicity of antibody conjugates in tumor spheroids. (A) CLSM images of NCI-ADR Res spheroids after incubation with Pab-640R and IgG-640R overnight. Scale bar, 100 μm. (B) Quantitative distribution of Pab-640R and IgG-640R in

    Article Snippet: Mouse IgG1 isotype control (IgG) was purchased from R & D Systems, Inc. (Minneapolis, MN, USA).

    Techniques: Confocal Laser Scanning Microscopy, Incubation

    IL-8 production in Caco-2 cells after treatment with TNF-α in the presence of antibodies to TNFR1 or TNFR2 in the basolateral chamber. α- TNFR1 antibody to TNF-α receptor 1 (15 μg/ml), α- TNFR2 antibody to TNF-α receptor 2 (15 μg/ml), IgG non-specific antibody (30 μg/ml). * p

    Journal: Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract

    Article Title: TNF-α Induces Vectorial Secretion of IL-8 in Caco-2 Cells

    doi: 10.1007/s11605-010-1321-9

    Figure Lengend Snippet: IL-8 production in Caco-2 cells after treatment with TNF-α in the presence of antibodies to TNFR1 or TNFR2 in the basolateral chamber. α- TNFR1 antibody to TNF-α receptor 1 (15 μg/ml), α- TNFR2 antibody to TNF-α receptor 2 (15 μg/ml), IgG non-specific antibody (30 μg/ml). * p

    Article Snippet: Recombinant human TNF-α, mouse monoclonal anti-human TNF receptor 1 (TNFR1) antibody, mouse monoclonal anti-human TNF receptor 2 (TNFR2) antibody, mouse IgG1 isotype control antibody and enzyme-linked immunosorbent assay (ELISA) kits were purchased from R & D Systems (Minneapolis, MN, USA).

    Techniques:

    IL-8 production in Caco-2 cells after treatment with TNF-α in the presence of antibodies to TNFR1 or TNFR2 in the apical chamber. α- TNFR1 antibody to TNF-α receptor 1 (15 μg/ml), α- TNFR2 antibody to TNF-α receptor 2 (15 μg/ml), IgG isotype control antibody (30 μg/ml). * p

    Journal: Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract

    Article Title: TNF-α Induces Vectorial Secretion of IL-8 in Caco-2 Cells

    doi: 10.1007/s11605-010-1321-9

    Figure Lengend Snippet: IL-8 production in Caco-2 cells after treatment with TNF-α in the presence of antibodies to TNFR1 or TNFR2 in the apical chamber. α- TNFR1 antibody to TNF-α receptor 1 (15 μg/ml), α- TNFR2 antibody to TNF-α receptor 2 (15 μg/ml), IgG isotype control antibody (30 μg/ml). * p

    Article Snippet: Recombinant human TNF-α, mouse monoclonal anti-human TNF receptor 1 (TNFR1) antibody, mouse monoclonal anti-human TNF receptor 2 (TNFR2) antibody, mouse IgG1 isotype control antibody and enzyme-linked immunosorbent assay (ELISA) kits were purchased from R & D Systems (Minneapolis, MN, USA).

    Techniques:

    Binding of immunized-goat antisera and MAbs to linear-synthetic biotinylated peptides by ELISA. (A and B) Human MAbs (HC33.4, HC84.26, and human IgG1 isotype control B6) at 5 μg/ml (A) or immunized-goat antisera from G757 diluted 1:50 (B) were

    Journal: Journal of Virology

    Article Title: Recombinant Hepatitis C Virus Envelope Glycoprotein Vaccine Elicits Antibodies Targeting Multiple Epitopes on the Envelope Glycoproteins Associated with Broad Cross-Neutralization

    doi: 10.1128/JVI.01911-14

    Figure Lengend Snippet: Binding of immunized-goat antisera and MAbs to linear-synthetic biotinylated peptides by ELISA. (A and B) Human MAbs (HC33.4, HC84.26, and human IgG1 isotype control B6) at 5 μg/ml (A) or immunized-goat antisera from G757 diluted 1:50 (B) were

    Article Snippet: Mouse anti-NS5A antibody (9E10) or mouse isotype control IgG1 (R & D Systems) was added at 1 μg/ml as a primary antibody, and an Alexa Fluor 647-conjugated goat anti-mouse secondary antibody was added (1:400; Molecular Probes).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

    Model of chitin-induced anti-inflammatory IL-1Ra and proinflammatory IL-1β responses. IgG-opsonized chitin is recognized by the Fc-γ receptor and uptake induced via the Syk/PI3K pathway, which results in isolated induction of IL-1Ra. In the presence of other Syk-independent PRR ligands, like Pam3Cys (P3Cys), LPS, and MDP, IgG-opsonized chitin induces IL-1β in a synergistic manner.

    Journal: mBio

    Article Title: Aspergillus Cell Wall Chitin Induces Anti- and Proinflammatory Cytokines in Human PBMCs via the Fc-γ Receptor/Syk/PI3K Pathway

    doi: 10.1128/mBio.01823-15

    Figure Lengend Snippet: Model of chitin-induced anti-inflammatory IL-1Ra and proinflammatory IL-1β responses. IgG-opsonized chitin is recognized by the Fc-γ receptor and uptake induced via the Syk/PI3K pathway, which results in isolated induction of IL-1Ra. In the presence of other Syk-independent PRR ligands, like Pam3Cys (P3Cys), LPS, and MDP, IgG-opsonized chitin induces IL-1β in a synergistic manner.

    Article Snippet: Isotype control goat IgG (10 µg/ml), anti-human β2 -integrin (anti-CR3 [10 µg/ml]), and isotype control mouse IgG2b (10 µg/ml) were obtained from R & D Systems Minneapolis, MN.

    Techniques: Isolation

    CXCL5 neutralization reduces the proliferation-promoting effect of ASCs. (A) Representative cytokine array analysis of the expression of 174 cytokines in the media from co-cultures of tumor cells with WI-38 cells (left columns) or ASCs (right columns). Arrow denotes the CXCL5 (also known as ENA-78) band. (B and C) Effects of neutralization of CXCL5 on the ASC-stimulated proliferation of (B) MCF-7 and (C) MDA-MB-231 cells. CXCL5 was neutralized with CXCL5 Nu-Ab, and a non-specific monoclonal IgG1 was used as the control antibody. WI-38 cells and HMECs were used as additional controls to highlight the specific effect of ASC-secreted CXCL5. All experiments were repeated 5 times. CXCL5, C-X-C motif ligand 5; CXCL5 Nu-Ab, CXCL5-specific neutralizing monoclonal antibody; Ig, immunoglobulin; ASC, adipose tissue-derived stem cells; HMEC, human mammary epithelial cell; ENA-78, epithelial cell-derived neutrophil-activating peptide-78.

    Journal: Oncology Letters

    Article Title: CXCL5 secreted from adipose tissue-derived stem cells promotes cancer cell proliferation

    doi: 10.3892/ol.2017.7522

    Figure Lengend Snippet: CXCL5 neutralization reduces the proliferation-promoting effect of ASCs. (A) Representative cytokine array analysis of the expression of 174 cytokines in the media from co-cultures of tumor cells with WI-38 cells (left columns) or ASCs (right columns). Arrow denotes the CXCL5 (also known as ENA-78) band. (B and C) Effects of neutralization of CXCL5 on the ASC-stimulated proliferation of (B) MCF-7 and (C) MDA-MB-231 cells. CXCL5 was neutralized with CXCL5 Nu-Ab, and a non-specific monoclonal IgG1 was used as the control antibody. WI-38 cells and HMECs were used as additional controls to highlight the specific effect of ASC-secreted CXCL5. All experiments were repeated 5 times. CXCL5, C-X-C motif ligand 5; CXCL5 Nu-Ab, CXCL5-specific neutralizing monoclonal antibody; Ig, immunoglobulin; ASC, adipose tissue-derived stem cells; HMEC, human mammary epithelial cell; ENA-78, epithelial cell-derived neutrophil-activating peptide-78.

    Article Snippet: Anti-CXCL5 treatment Co-cultured ASCs and tumor cells at the density of 1×104 /cm2 were incubated at 37°C with an anti-human CXCL5 monoclonal antibody (catalog no., MAB 254, R & D Systems) diluted to 2.5 µg/ml to neutralize CXCL5 or with a mouse monoclonal IgG1 isotype control diluted to 2.5 µg/ml (catalog no., MAB 002; R & D Systems, Minneapolis, MN, USA) for 4 days, which were placed in the upper and lower sides of the chamber to inhibit all possible functions of CXCL5.

    Techniques: Neutralization, Expressing, Multiple Displacement Amplification, Derivative Assay

    AGE-LDL increased cell surface expression of CCR2. Macrophages were incubated in the presence of either LDL or AGE-LDL for 48 hours, and CCR2 surface expression was determined by flow cytometry with anti-CCR2 IgG and expressed as specific mean fluorescence intensity (MFI). Data are shown as mean ± SEM (n=3). The experiments were done twice for each donor.

    Journal: Atherosclerosis

    Article Title: Glycated LDL increases monocyte CC chemokine receptor 2 expression and monocyte chemoattractant protein-1-mediated chemotaxis

    doi: 10.1016/j.atherosclerosis.2007.10.035

    Figure Lengend Snippet: AGE-LDL increased cell surface expression of CCR2. Macrophages were incubated in the presence of either LDL or AGE-LDL for 48 hours, and CCR2 surface expression was determined by flow cytometry with anti-CCR2 IgG and expressed as specific mean fluorescence intensity (MFI). Data are shown as mean ± SEM (n=3). The experiments were done twice for each donor.

    Article Snippet: An anti-human Receptor for AGE (RAGE) mouse monoclonal antibody and its IgG2b isotype control (R & D systems) served to test the involvement of RAGE by blocking receptor-ligand interactions.

    Techniques: Expressing, Incubation, Flow Cytometry, Cytometry, Fluorescence

    U2AF1 binds RNA in the cytoplasm. ( A ) Single molecule FISH analysis of IL8 mRNA expression in wt/wt, wt/S34F mutant cells plotted as frequency histogram of IL8 mRNA per cell. ( Inset ) Single molecule RNAFISH image (gray) showing heterogeneity in IL8 RNA expression and nuclei counter stained with DAPI (blue) in wt/wt cells. ( B ) Quantitative immunofluorescence detection of cytoplasmic U2AF1 (gray) in PFA fixed cells showing heterogeneity in U2AF1 distribution in the cytoplasm and nuclei counterstained with DAPI (blue). ( C ) Box plot (min, max, first and third quartiles, and median) showing the fraction of U2AF1 in the nucleus and cytoplasm of individual wt/wt and wt/S34F cells. ( D ) Western blot analysis showing presence of U2AF1 in the cytosolic fraction. RBM10 and RPA32 (increasing amounts) served as controls for any nuclear protein contamination in the cytoplasmic fraction. ( E ) U2AF1 inteacts with mature IL8 mRNA in the cytoplasm. RNA isolated from immunoprecipitates of cytoplasmic U2AF1 ( B ) was analyzed by RT-qPCR for association of IL8, IL1α, DNA polH, and RPB3 mRNA. Fraction of IL1α, IL8, DNA polH, and RPB3 mRNA bound to U2AF1 ( top ) and their relative enrichment to U2AF1 in mutant cells ( bottom ). ( F ) Transcriptome-wide analysis of WT U2AF1 bound cytoplasmic mature polyadenylated mRNA by RIP-seq. Scatter plot of enrichment of U2AF1 bound RNA (gray), intron-less Histone RNA (red) and mTOR regulated RNA (blue). ( G ) Box plot analysis of mRNA enriched in RIP over input or control IgG in the top decile of RIP, mTOR sensitive mRNA, EIF4A sensitive mRNA, or background mRNA. ( H ) 3′ splice site-like “AG” sequence and polypyrimidine-rich TOP motifs in 5′-UTRs of mTOR-regulated mRNAs bound by cytoplasmic U2AF1. Gene ontology terms of wild-type U2AF1-enriched cytoplasmic mRNA.

    Journal: Genes & Development

    Article Title: The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation

    doi: 10.1101/gad.319590.118

    Figure Lengend Snippet: U2AF1 binds RNA in the cytoplasm. ( A ) Single molecule FISH analysis of IL8 mRNA expression in wt/wt, wt/S34F mutant cells plotted as frequency histogram of IL8 mRNA per cell. ( Inset ) Single molecule RNAFISH image (gray) showing heterogeneity in IL8 RNA expression and nuclei counter stained with DAPI (blue) in wt/wt cells. ( B ) Quantitative immunofluorescence detection of cytoplasmic U2AF1 (gray) in PFA fixed cells showing heterogeneity in U2AF1 distribution in the cytoplasm and nuclei counterstained with DAPI (blue). ( C ) Box plot (min, max, first and third quartiles, and median) showing the fraction of U2AF1 in the nucleus and cytoplasm of individual wt/wt and wt/S34F cells. ( D ) Western blot analysis showing presence of U2AF1 in the cytosolic fraction. RBM10 and RPA32 (increasing amounts) served as controls for any nuclear protein contamination in the cytoplasmic fraction. ( E ) U2AF1 inteacts with mature IL8 mRNA in the cytoplasm. RNA isolated from immunoprecipitates of cytoplasmic U2AF1 ( B ) was analyzed by RT-qPCR for association of IL8, IL1α, DNA polH, and RPB3 mRNA. Fraction of IL1α, IL8, DNA polH, and RPB3 mRNA bound to U2AF1 ( top ) and their relative enrichment to U2AF1 in mutant cells ( bottom ). ( F ) Transcriptome-wide analysis of WT U2AF1 bound cytoplasmic mature polyadenylated mRNA by RIP-seq. Scatter plot of enrichment of U2AF1 bound RNA (gray), intron-less Histone RNA (red) and mTOR regulated RNA (blue). ( G ) Box plot analysis of mRNA enriched in RIP over input or control IgG in the top decile of RIP, mTOR sensitive mRNA, EIF4A sensitive mRNA, or background mRNA. ( H ) 3′ splice site-like “AG” sequence and polypyrimidine-rich TOP motifs in 5′-UTRs of mTOR-regulated mRNAs bound by cytoplasmic U2AF1. Gene ontology terms of wild-type U2AF1-enriched cytoplasmic mRNA.

    Article Snippet: Anti-IL8 and isotype control IgG1 (R & D Systems) were given to the mice via intraperitoneal injection three times a week (Monday, Wednesday, and Friday) starting 1 d after the tail vein injection for 10 wk.

    Techniques: Fluorescence In Situ Hybridization, Expressing, Mutagenesis, RNA Expression, Staining, Immunofluorescence, Western Blot, Isolation, Quantitative RT-PCR, Sequencing

    The effect of type I IFNs to SOCS2 mRNA induction in human moDCs. moDCs were exposed to LPS (A) for indicated time periods for mRNA measurement of IFNα, IFNβ, IFNγ and IFNλ1 or incubated with IFNα (Bi), IFNβ (Bii), IFNγ (Biii) and IFNλ1 (Biv) for indicated time periods for SOCS2 mRNA measurement by qRT-PCR. (C) moDCs were pretreated for 30 min with or without 30 ug/ml neutralizing anti-IFNR2 antibodies or IgG 2a isotype control mAb and then incubated for 4 h with or without LPS for SOCS2 mRNA measurement by qRT-PCR. Data shown are representative of three independent experiments and expressed as the –fold induction of the gene of interest at different time points compared to time point 0 where the values were set to 1(A–B) or different treatment conditions compared with medium that values were set to 1. Statistical significance of results with anti-IFNAR2 mAb compared with control mAb is indicated (C) (* p

    Journal: PLoS ONE

    Article Title: LPS Regulates SOCS2 Transcription in a Type I Interferon Dependent Autocrine-Paracrine Loop

    doi: 10.1371/journal.pone.0030166

    Figure Lengend Snippet: The effect of type I IFNs to SOCS2 mRNA induction in human moDCs. moDCs were exposed to LPS (A) for indicated time periods for mRNA measurement of IFNα, IFNβ, IFNγ and IFNλ1 or incubated with IFNα (Bi), IFNβ (Bii), IFNγ (Biii) and IFNλ1 (Biv) for indicated time periods for SOCS2 mRNA measurement by qRT-PCR. (C) moDCs were pretreated for 30 min with or without 30 ug/ml neutralizing anti-IFNR2 antibodies or IgG 2a isotype control mAb and then incubated for 4 h with or without LPS for SOCS2 mRNA measurement by qRT-PCR. Data shown are representative of three independent experiments and expressed as the –fold induction of the gene of interest at different time points compared to time point 0 where the values were set to 1(A–B) or different treatment conditions compared with medium that values were set to 1. Statistical significance of results with anti-IFNAR2 mAb compared with control mAb is indicated (C) (* p

    Article Snippet: Mouse anti-human IFNAR2 neutralizing antibodies (CD118; PBL Biomedical Laboratories, NJ, USA) and mouse IgG2a isotype controls (R & D systems, USA) were used at the concentration of 30 µg/ml.

    Techniques: Incubation, Quantitative RT-PCR

    Reactivation of OM-specific T-cell responses by dual blockade of the PD-1 and LAG-3 pathways. PBMCs were isolated from the blood of A. marginale -challenged cattle at 34 dpi and cultured in triplicate for 7 days with 0.2 μg/ml OM antigen and no antibody or 10 μg/ml of one or two blocking antibodies, including anti-PD-L1 MAb, anti-LAG-3 MAb, and control rat IgG, to reach a total concentration of 20 μg/ml IgG in the presence of OM antigen. Medium and uRBC antigen were included as negative controls. (A) PBMC proliferation presented as Δcpm values for each animal after subtracting the mean counts per minute for cells cultured with uRBCs from the mean counts per minute for cells cultured with OMs. (B) Supernatants were harvested from the proliferation assay mixture on day 6 and pooled, and IFN-γ production for each animal was measured by an ELISA in duplicate. Bars indicate group mean responses. Asterisks indicate significant differences between control IgG and anti-LAG-3 MAb plus anti-PD-L1 MAb, where the P value was

    Journal: Infection and Immunity

    Article Title: Cooperation of PD-1 and LAG-3 Contributes to T-Cell Exhaustion in Anaplasma marginale-Infected Cattle

    doi: 10.1128/IAI.00278-16

    Figure Lengend Snippet: Reactivation of OM-specific T-cell responses by dual blockade of the PD-1 and LAG-3 pathways. PBMCs were isolated from the blood of A. marginale -challenged cattle at 34 dpi and cultured in triplicate for 7 days with 0.2 μg/ml OM antigen and no antibody or 10 μg/ml of one or two blocking antibodies, including anti-PD-L1 MAb, anti-LAG-3 MAb, and control rat IgG, to reach a total concentration of 20 μg/ml IgG in the presence of OM antigen. Medium and uRBC antigen were included as negative controls. (A) PBMC proliferation presented as Δcpm values for each animal after subtracting the mean counts per minute for cells cultured with uRBCs from the mean counts per minute for cells cultured with OMs. (B) Supernatants were harvested from the proliferation assay mixture on day 6 and pooled, and IFN-γ production for each animal was measured by an ELISA in duplicate. Bars indicate group mean responses. Asterisks indicate significant differences between control IgG and anti-LAG-3 MAb plus anti-PD-L1 MAb, where the P value was

    Article Snippet: Isotype control mouse IgG1 was stained by using a goat anti-mouse IgG1-PE-Cy5.5 conjugate (catalog number ; Invitrogen).

    Techniques: Isolation, Cell Culture, Blocking Assay, Concentration Assay, Proliferation Assay, Enzyme-linked Immunosorbent Assay

    Role of glycosylation and keratin binding in Srr1-mediated GBS cell attachment. (A) Adherence of GBS strains NCTC10/84 and the Δ srr-1 mutant to VK2/E6E7 vaginal epithelial cells preincubated with anti-cytokeratin peptide 4 antibody (KT4) or an IgG1 isotope control. (B) Adherence of GBS strains NCTC10/84 and the Δ srr-1 mutant to VK2/E6E7 vaginal epithelial cells preincubated with wheat germ agglutinin (WGA). All experiments were repeated at least two times in triplicate; data from a representative experiment are shown. Error bars indicate 95% confidence intervals of mean values from three wells. *, P

    Journal: Journal of Bacteriology

    Article Title: Serine-Rich Repeat Proteins and Pili Promote Streptococcus agalactiae Colonization of the Vaginal Tract ▿

    doi: 10.1128/JB.00094-11

    Figure Lengend Snippet: Role of glycosylation and keratin binding in Srr1-mediated GBS cell attachment. (A) Adherence of GBS strains NCTC10/84 and the Δ srr-1 mutant to VK2/E6E7 vaginal epithelial cells preincubated with anti-cytokeratin peptide 4 antibody (KT4) or an IgG1 isotope control. (B) Adherence of GBS strains NCTC10/84 and the Δ srr-1 mutant to VK2/E6E7 vaginal epithelial cells preincubated with wheat germ agglutinin (WGA). All experiments were repeated at least two times in triplicate; data from a representative experiment are shown. Error bars indicate 95% confidence intervals of mean values from three wells. *, P

    Article Snippet: A mouse IgG1 isotope control (R & D Systems) was used as a negative control.

    Techniques: Binding Assay, Cell Attachment Assay, Mutagenesis, Whole Genome Amplification

    Effect of TNFα neutralization on pro-inflammatory cytokine release from lung tissue. Notes: The effect of pre-exposure to neutralizing TNFα IgG antibody (nTNFα IgG) and control IgG antibody (Isotype IgG) (both 1 μg/mL) on CCL5 ( A ) and IL-6 ( B ) release from whole tissue explants from COPD patients (n=6) that were either unstimulated (control) or stimulated with poly(I:C) (100 μg/mL) or R848 (10 μg/mL) for 24 hours. Data are presented as median with range (CCL5) or mean with SEM (IL-6). *, ** Refer to significantly below untreated levels ( P

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    Article Title: Characterization of TLR-induced inflammatory responses in COPD and control lung tissue explants

    doi: 10.2147/COPD.S105156

    Figure Lengend Snippet: Effect of TNFα neutralization on pro-inflammatory cytokine release from lung tissue. Notes: The effect of pre-exposure to neutralizing TNFα IgG antibody (nTNFα IgG) and control IgG antibody (Isotype IgG) (both 1 μg/mL) on CCL5 ( A ) and IL-6 ( B ) release from whole tissue explants from COPD patients (n=6) that were either unstimulated (control) or stimulated with poly(I:C) (100 μg/mL) or R848 (10 μg/mL) for 24 hours. Data are presented as median with range (CCL5) or mean with SEM (IL-6). *, ** Refer to significantly below untreated levels ( P

    Article Snippet: TNFα neutralization Human TNFα Antibody (Clone #28401) and Mouse IgG1 Isotype (Clone #11711) were purchased from R & D Systems Europe (Abingdon, UK), and the stock solutions were prepared according to the manufacturers’ instructions.

    Techniques: Neutralization

    TE-7 stains cells below the epidermis in normal skin tissue. Human dermis was collected from breast tissue, fixed, and paraffin-embedded. Slides were cut and stained with an IgG1 isotype control or the TE-7 antibody as described in Materials and Methods.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: An Immunohistochemical Method for Identifying Fibroblasts in Formalin-fixed, Paraffin-embedded Tissue

    doi: 10.1369/jhc.7A7287.2007

    Figure Lengend Snippet: TE-7 stains cells below the epidermis in normal skin tissue. Human dermis was collected from breast tissue, fixed, and paraffin-embedded. Slides were cut and stained with an IgG1 isotype control or the TE-7 antibody as described in Materials and Methods.

    Article Snippet: Isotype control antibodies were mouse IgG (I-2000; Vector Laboratories), mouse IgG1 (CBL600; Chemicon, Temecula, CA), mouse IgG2a (CBL601; Chemicon), mouse IgG3 (M9019; Sigma-Aldrich, St Louis, MO), mouse IgM (PP50; Chemicon), and rabbit IgG (011-000-003; Jackson Immunoresearch Laboratories).

    Techniques: Staining