mouse igg isotype control Search Results


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  • 99
    Thermo Fisher mouse igg isotype control
    Mouse Igg Isotype Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems mouse igg isotype control
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    Thermo Fisher normal mouse igg
    Normal Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher igg3
    Serum <t>IgG</t> immune response in mice inoculated with T1 C. pseudotuberculosis strain, as evaluated by ELISA. Graph represents means of Optical Density (OD) values found for each group ( n = 5 animals for group). Results are representative of the mean values obtained from two experiments. a . BALB/c mice were inoculated with increasing dosages: 5x10 5 , 1x10 6 , 5x10 6 and 1x10 7 CFU. Blood was collected 120 days after inoculation. Data were analyzed by ANOVA and Tukey post-hoc tests; *, †, ‡ and § indicate pairs with statistically significant differences. b . IgG subclass <t>(IgG1,</t> <t>IgG2a,</t> <t>IgG2b</t> and <t>IgG3)</t> production throughout the course of the experiment: control (before infection), 7, 30, 60, and 120 days after infection. Mice were inoculated with 10 7 CFU of T1 strain of C. pseudotuberculosis . Data were analyzed by ANOVA. * P
    Igg3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Purified Mouse IgG2a Isotype Control
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    FITC Conjugated Mouse IgG1 Kappa Light Chain Isotype Control antibody
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    MOUSE IgG2b isotype control 010 0142
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    MOUSE IgG3 Kappa κ isotype control 010 001 336
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    Serum IgG immune response in mice inoculated with T1 C. pseudotuberculosis strain, as evaluated by ELISA. Graph represents means of Optical Density (OD) values found for each group ( n = 5 animals for group). Results are representative of the mean values obtained from two experiments. a . BALB/c mice were inoculated with increasing dosages: 5x10 5 , 1x10 6 , 5x10 6 and 1x10 7 CFU. Blood was collected 120 days after inoculation. Data were analyzed by ANOVA and Tukey post-hoc tests; *, †, ‡ and § indicate pairs with statistically significant differences. b . IgG subclass (IgG1, IgG2a, IgG2b and IgG3) production throughout the course of the experiment: control (before infection), 7, 30, 60, and 120 days after infection. Mice were inoculated with 10 7 CFU of T1 strain of C. pseudotuberculosis . Data were analyzed by ANOVA. * P

    Journal: BMC Veterinary Research

    Article Title: Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

    doi: 10.1186/s12917-016-0811-8

    Figure Lengend Snippet: Serum IgG immune response in mice inoculated with T1 C. pseudotuberculosis strain, as evaluated by ELISA. Graph represents means of Optical Density (OD) values found for each group ( n = 5 animals for group). Results are representative of the mean values obtained from two experiments. a . BALB/c mice were inoculated with increasing dosages: 5x10 5 , 1x10 6 , 5x10 6 and 1x10 7 CFU. Blood was collected 120 days after inoculation. Data were analyzed by ANOVA and Tukey post-hoc tests; *, †, ‡ and § indicate pairs with statistically significant differences. b . IgG subclass (IgG1, IgG2a, IgG2b and IgG3) production throughout the course of the experiment: control (before infection), 7, 30, 60, and 120 days after infection. Mice were inoculated with 10 7 CFU of T1 strain of C. pseudotuberculosis . Data were analyzed by ANOVA. * P

    Article Snippet: To evaluate IgG1, IgG2a, IgG2b and IgG3, wells were filled with 50 μL/well of HRP conjugated rabbit Ig antimouse IgG1, IgG2a, IgG2b or IgG3 (Zymed, San Francisco, CA, USA), respectively, each diluted at 1:8.000 in 1 % skim milk/PBS-T. All plates were then incubated for 45 min at 37 o C. Each plate was washed five times in PBS-T and 50 μL/well of Citrate Phosphate Buffer pH 5.1, ortho-phenyl-diamine (Sigma, St Louis, MO, USA) and 30 % H2 O2 ] were added and left for 15 min at room temperature in a dark chamber.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Infection