Journal: Molecular Medicine Reports
Article Title: Role of the complement anaphylatoxin C5a-receptor pathway in atopic dermatitis in mice
Figure Lengend Snippet: Inhibitory mechanism of C5aRA on AD. Following DNCB application onto mouse dorsal skin for 2 weeks, C5aR expression in skin tissue was significantly increased and the infiltration of mast cells was significantly enhanced as well. The activated mast cells released polarizing cytokines to increase IL-4 production from Th2 and IFN-γ production from mixed Th1/Th2. In addition, the increased C5aR expression on mast cells enhanced IgE in B cells. At the same time, mast cells released histamine to generate pruritus symptoms. Intracutaneous injection of C5aRA directly prevented the binding of C5a to C5aR on mast cells, then decreased the skin-fold thickness, number of infiltrating leukocytes and mast cells as well as levels of IL-4, IFN-γ, histamine and IgE, and thereby inhibited the symptoms of AD. AD, atopic dermatitis; DNCB, 2,4-dinitrochlorobenzene; Th, T helper; IL, interleukin; IFN, interferon; IgE, immunoglobulin E; C5aRA, C5a receptor antagonist.
Article Snippet: Production of IL-4 and IFN-γ in skin tissue and IL-4, IFN-γ, histamine and IgE levels in serum were determined using ELISA kits (Mouse IL-4, Mouse IFN-γ, Porcine Histamine HIS and Mouse IgE ELISA kits; Sigma-Aldrich) according to the manufacturer’s instructions.
Techniques: Expressing, Injection, Binding Assay