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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Transcriptional Activators and Repressors for the Neuron-specific Expression of a Metabotropic Glutamate Receptor
doi: 10.1074/jbc.m700149200
Figure Lengend Snippet: FIGURE 1. Characterization of Grm1 TISs, genomic structure, and analysis of alternative first exon expression. A, annealed sequences of human and mouse Grm1 exon Ia, as obtained by 5-RACE and public sequence data bases. The digits associated with nucleotides indicate the number of 5-RACE clones starting at that position. The height of histograms is proportional to the number of clones. The start positions of known cDNAs or expressed sequence tags are indicated by their accession number or reference. Splice donors are indicated in boldface type. B, RNase protection assay. Multiple protected bands are generated by probes encoding exon Ia and II, whereas a single band is generated by the control -actin probe (filled arrowheads, lanes 1, 3, and 5). Undigested probes are shown (open arrowheads). A high molecular weight self-annealing product (asterisk, lane 2) was produced by the undigested exon Ia probe, which however did not produce nonspecific bands due to self-protection (lane 7). C, complete genomic structure of mouse and human Grm1, based on 5-RACE data and public genomic, cDNA, and expressed sequence tag sequences. Alignments were performed by BLAST 2.0. Protein coding regions and 5- or 3-untrans- lated regions are indicated by open and gray boxes, respectively. Constitutive and alternative splicing are indicated by filled and dashed lines, respectively. The size of exons is given in bp and in kbp (in italic type) for introns. D, regional distribution of Grm1 transcripts generated from alternative first exons. The experiments shown are representative of at least four replicates. OB, olfactory bulb; CTX, cortex; HIP, hippocampus; AMY, amygdala; STR, striatum; CRB, cerebellum.
Article Snippet: 5 -Rapid Amplification of cDNA Ends (RACE)—The transcription initiation sites (TISs) of the human andmousemGlu1 gene were determined by 5 -RACE using whole mouse brain and human hippocampal and
Techniques: Expressing, Sequencing, Clone Assay, Rnase Protection Assay, Generated, Control, High Molecular Weight, Produced, Alternative Splicing