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    Millipore antibodies mouse antineun
    Distribution of <t>IGFBP2‐positive</t> cells and total cell counts and morphology in the hippocampus of wild‐type and mutant mice. A–E) IGFBP2 neurons and astrocytes in the hippocampus of wild‐type mice ( igfbp2 +/+ ). Arrows in B, neurons expressing IGFBP2 and <t>NeuN;</t> open arrows in C, small neurons expressing GAD67 and IGFBP2; solid arrows, larger neurons expressing GAD67 without IGFBP2, D) Astrocytes expressing IGFBP2, E) Neurons expressing IGFBP2 in wild‐type and not in igfbp2 −/− mice. F) DAPI staining in the hippocampus of wild‐type and igfbp2 −/− mice. G,H) Staining with DAPI, NeuN, and GAD67 in CA1 from wild‐type and igfbp2 −/− mice at p15 and p45. I,J) Quantification of total cell number and optical density in the stratum oriens (SO), stratum pyramidale (SP), and stratum radiatum (SR) of CA1, CA3, and the DG ( n = 4 mice). K) Expression of IGFBP2, NR2B, and SPAR. L–O) Golgi‐stained neurons in cortex and hippocampus. K) * P
    Antibodies Mouse Antineun, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Distribution of IGFBP2‐positive cells and total cell counts and morphology in the hippocampus of wild‐type and mutant mice. A–E) IGFBP2 neurons and astrocytes in the hippocampus of wild‐type mice ( igfbp2 +/+ ). Arrows in B, neurons expressing IGFBP2 and NeuN; open arrows in C, small neurons expressing GAD67 and IGFBP2; solid arrows, larger neurons expressing GAD67 without IGFBP2, D) Astrocytes expressing IGFBP2, E) Neurons expressing IGFBP2 in wild‐type and not in igfbp2 −/− mice. F) DAPI staining in the hippocampus of wild‐type and igfbp2 −/− mice. G,H) Staining with DAPI, NeuN, and GAD67 in CA1 from wild‐type and igfbp2 −/− mice at p15 and p45. I,J) Quantification of total cell number and optical density in the stratum oriens (SO), stratum pyramidale (SP), and stratum radiatum (SR) of CA1, CA3, and the DG ( n = 4 mice). K) Expression of IGFBP2, NR2B, and SPAR. L–O) Golgi‐stained neurons in cortex and hippocampus. K) * P

    Journal: Advanced Science

    Article Title: IGFBP2 Plays an Essential Role in Cognitive Development during Early Life, IGFBP2 Plays an Essential Role in Cognitive Development during Early Life

    doi: 10.1002/advs.201901152

    Figure Lengend Snippet: Distribution of IGFBP2‐positive cells and total cell counts and morphology in the hippocampus of wild‐type and mutant mice. A–E) IGFBP2 neurons and astrocytes in the hippocampus of wild‐type mice ( igfbp2 +/+ ). Arrows in B, neurons expressing IGFBP2 and NeuN; open arrows in C, small neurons expressing GAD67 and IGFBP2; solid arrows, larger neurons expressing GAD67 without IGFBP2, D) Astrocytes expressing IGFBP2, E) Neurons expressing IGFBP2 in wild‐type and not in igfbp2 −/− mice. F) DAPI staining in the hippocampus of wild‐type and igfbp2 −/− mice. G,H) Staining with DAPI, NeuN, and GAD67 in CA1 from wild‐type and igfbp2 −/− mice at p15 and p45. I,J) Quantification of total cell number and optical density in the stratum oriens (SO), stratum pyramidale (SP), and stratum radiatum (SR) of CA1, CA3, and the DG ( n = 4 mice). K) Expression of IGFBP2, NR2B, and SPAR. L–O) Golgi‐stained neurons in cortex and hippocampus. K) * P

    Article Snippet: The following primary antibodies were used: goat anti‐IGFBP2 (1:200, Santa Cruz), mouse anti‐NeuN (1:1000; Millipore), mouse anti‐GAD67 (1:500, Millipore), and anti‐GFAP (1:500, Abcam).

    Techniques: Mutagenesis, Mouse Assay, Expressing, Staining

    Spatial distribution and cell type of CYLD ischemic cortical borders after 2 h of focal cerebral ischemia and 72 h of reperfusion, n = 6 (Scale bar = 100 μm). (A) Co-expression of CYLD (green) and NeuN (red, neurons). (B) Microglia co-expressing CYLD (green) and Iba1 (red, microglia). (C) Astrocytes co-expressing CYLD (green) and GFAP (red, astrocytes). 1–3 were magnified from a merged image and show CYLD. Yellow arrows indicate co-expression between CYLD and neurons (Scale bar = 50 μm).

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats

    doi: 10.3389/fnmol.2017.00363

    Figure Lengend Snippet: Spatial distribution and cell type of CYLD ischemic cortical borders after 2 h of focal cerebral ischemia and 72 h of reperfusion, n = 6 (Scale bar = 100 μm). (A) Co-expression of CYLD (green) and NeuN (red, neurons). (B) Microglia co-expressing CYLD (green) and Iba1 (red, microglia). (C) Astrocytes co-expressing CYLD (green) and GFAP (red, astrocytes). 1–3 were magnified from a merged image and show CYLD. Yellow arrows indicate co-expression between CYLD and neurons (Scale bar = 50 μm).

    Article Snippet: Sections were subsequently incubated with prepared primary antibodies at 4°C overnight as follows: Anti-NeuN mouse (labeled neurons, MAB377, Millipore, 1:100), anti-GFAP mouse (labeled astrocytes, BM0055, Boster, 1:100), anti-Iba1 goat (to label microglia, NB100-1028SS, Novus, 1:50), anti-CYLD rabbit (11110-1-AP, Proteintech, 1:100), anti-CYLD mouse (SC-74435, Santa Cruz, 1:100), anti-NF-κB p65 rabbit (#8242, Cell Signaling Technology, 1:50) and anti-CX3CL1 rabbit (ab25088, Abcam, 1:100).

    Techniques: Expressing

    The neuroprotective effects of minoxidil on the neurite outgrowth of dorsal root ganglion (DRG) neurons. To test neuroprotective effects of minoxidil, primary culture of DRG neurons from 7-weeks-old C57/B6J mice were pre-treated with minoxidil for 24 hours and then exposed to 0.1 or 0.01 μM paclitaxel for another 24 hours. Minoxidil exhibited a significant neuroprotective effect against paclitaxel with regard to neurite outgrowth in DRG neurons. ( a ) Representative images show that minoxidil is a potential neuroprotective drug in vitro DRG neuron model. Scale bar, 50 μm. Green, anti-β-III tubulin antibody. Red, anti-NeuN antibody. ( b ) Quantitative analyses of neurite outgrowth in DRG neurons. (c) Quantitative analyses of neural viability in DRG neurons. Each value represents the mean ± S.E.M. from at least 5 different experiments. ***P

    Journal: Scientific Reports

    Article Title: Minoxidil is a potential neuroprotective drug for paclitaxel-induced peripheral neuropathy

    doi: 10.1038/srep45366

    Figure Lengend Snippet: The neuroprotective effects of minoxidil on the neurite outgrowth of dorsal root ganglion (DRG) neurons. To test neuroprotective effects of minoxidil, primary culture of DRG neurons from 7-weeks-old C57/B6J mice were pre-treated with minoxidil for 24 hours and then exposed to 0.1 or 0.01 μM paclitaxel for another 24 hours. Minoxidil exhibited a significant neuroprotective effect against paclitaxel with regard to neurite outgrowth in DRG neurons. ( a ) Representative images show that minoxidil is a potential neuroprotective drug in vitro DRG neuron model. Scale bar, 50 μm. Green, anti-β-III tubulin antibody. Red, anti-NeuN antibody. ( b ) Quantitative analyses of neurite outgrowth in DRG neurons. (c) Quantitative analyses of neural viability in DRG neurons. Each value represents the mean ± S.E.M. from at least 5 different experiments. ***P

    Article Snippet: DRG tissue samples were co-stained with mouse anti-NeuN antibody (1:100; Millipore), mouse anti-NOS2 (1:100; Santa Cruz), mouse anti-Iba-1 (1:100; Santa Cruz), rabbit anti-Arginase-1 (1:100; Santa Cruz), rabbit anti-Iba-1 (1:100; Abcam) and rat anti-CD68 antibody (1:100; Novus, USA) at 4 °C overnight.

    Techniques: Mouse Assay, In Vitro

    Immunofluorescent labeling for glial cells (A, GFAP–green) and neuronal cells (B, NeuN–red) in CA1 (adjacent area of injection) of the control and NMDA groups 1 and 4 weeks after injection . Nuclei labeled with DAPI (blue).

    Journal: Frontiers in Neuroscience

    Article Title: Differential Expression of AMPA Subunits Induced by NMDA Intrahippocampal Injection in Rats

    doi: 10.3389/fnins.2016.00032

    Figure Lengend Snippet: Immunofluorescent labeling for glial cells (A, GFAP–green) and neuronal cells (B, NeuN–red) in CA1 (adjacent area of injection) of the control and NMDA groups 1 and 4 weeks after injection . Nuclei labeled with DAPI (blue).

    Article Snippet: Tissue sections were successively washed and incubated at room temperature with primary antibodies mouse anti-NeuN (1:1000, Millipore) and mouse anti-GFAP (1:1000, Sigma) to detect neurons and glial cells, respectively.

    Techniques: Labeling, Injection

    Fluorescent photomicrographs show both neurons and astrocytes express BDNF, but not microglia . (A–D) Colocalization of BDNF (green) and NeuN (red), NeuN is the marker for neurons. (E–H) Colocalization of BDNF (green) and GFAP (red), GFAP is the marker for astrocytes. (I–L) Expression of BDNF (green) and OX42 (red), OX42 is the marker for microglia. The framed areas in (C , G , K) are magnified in (D , H , L) respectively. Arrows indicate the colocalization of BDNF/NeuN (D) and BDNF/GFAP (H) ; arrowheads indicate the single expression of NeuN (D) or OX42 (L) . Scale bars = 20 μm in (K) (applies A–C , E–G , I–K ); 10 μm in (H) (applies D , H , L ).

    Journal: Frontiers in Neural Circuits

    Article Title: Neurochemical properties of BDNF-containing neurons projecting to rostral ventromedial medulla in the ventrolateral periaqueductal gray

    doi: 10.3389/fncir.2014.00137

    Figure Lengend Snippet: Fluorescent photomicrographs show both neurons and astrocytes express BDNF, but not microglia . (A–D) Colocalization of BDNF (green) and NeuN (red), NeuN is the marker for neurons. (E–H) Colocalization of BDNF (green) and GFAP (red), GFAP is the marker for astrocytes. (I–L) Expression of BDNF (green) and OX42 (red), OX42 is the marker for microglia. The framed areas in (C , G , K) are magnified in (D , H , L) respectively. Arrows indicate the colocalization of BDNF/NeuN (D) and BDNF/GFAP (H) ; arrowheads indicate the single expression of NeuN (D) or OX42 (L) . Scale bars = 20 μm in (K) (applies A–C , E–G , I–K ); 10 μm in (H) (applies D , H , L ).

    Article Snippet: The antibodies used in the current study were rabbit anti-BDNF antiserum (ab6201; Abcam, Cambridge, MA, USA); mouse anti-NeuN antiserum (MAB377; Millipore, Billerica, MA, USA); mouse anti-GFAP antiserum (MAB3402; Millipore); mouse anti-OX42 antiserum (CBL1512; Millipore); guinea pig anti-FG antiserum (NM-101; PROTOS BIOTECH CORP, New York, NY, USA); goat anti-5-HT antiserum (20079; ImmunoStar, Houston, Texas, USA); rat anti-NT antiserum (NP-103; PROTOS BIOTECH CORP); rat anti-SP antiserum (MAB356; Millipore); goat anti-CGRP antiserum (ab36001; Abcam); mouse anti-NOS antiserum (N-2280; Sigma, St. Louis, MO, USA); mouse anti-PV antiserum (P-3171; Sigma); mouse anti-TH antiserum (T-2928; Sigma); goat anti-TrkB antiserum (sc-20542; Santa Cruz Biotechnology, Santa Cruz, CA, USA); mouse anti-FOS antiserum (ab11959; Abcam); biotin-donkey anti-rabbit IgG (AP182F; Millipore); Alexa594-donkey anti-mouse IgG (A21203; Invitrogen, Carlsbad, CA, USA); Alexa594-goat anti-guinea pig IgG (A-11076; Invitrogen); Alexa594-donkey anti-goat IgG (A-11058; Invitrogen); Cy3-donkey anti-rat IgG (AP189c; Millipore); Alexa647-donkey anti-guinea pig IgG (AP193SA6; Millipore); FITC-Avidin (A-2001; Vector, Burlingame, CA, USA).

    Techniques: Marker, Expressing

    A-B Analysis of hippocampal neurogenesis in TNFR1 −/− mice 3 weeks after CCI. A , The % of BrdU/NeuN double-positive cells was significantly higher in the GCL of TNFR1 −/− than wilt-type mice and similar to shams. Mean ±

    Journal: Brain, behavior, and immunity

    Article Title: NEUROPATHIC PAIN-INDUCED DEPRESSIVE-LIKE BEHAVIOR AND HIPPOCAMPAL NEUROGENESIS AND PLASTICITY ARE DEPENDENT ON TNFR1 SIGNALING

    doi: 10.1016/j.bbi.2014.04.003

    Figure Lengend Snippet: A-B Analysis of hippocampal neurogenesis in TNFR1 −/− mice 3 weeks after CCI. A , The % of BrdU/NeuN double-positive cells was significantly higher in the GCL of TNFR1 −/− than wilt-type mice and similar to shams. Mean ±

    Article Snippet: For NeuN and BrdU double-labeling, sections were incubated with mouse anti-NeuN primary antibody (1:100; Millipore), followed by anti-mouse Alexa 594-conjugated IgG (1:750, Invitrogen).

    Techniques: Mouse Assay

    Analysis of adult neurogenesis in the hippocampus and olfactory bulbs 3 and 12 weeks after injury. A , The number of BrdU + cells was similar between CCI and sham mice but the number of BrdU + /NeuN + cells were dramatically reduced in the GCL of CCI mice

    Journal: Brain, behavior, and immunity

    Article Title: NEUROPATHIC PAIN-INDUCED DEPRESSIVE-LIKE BEHAVIOR AND HIPPOCAMPAL NEUROGENESIS AND PLASTICITY ARE DEPENDENT ON TNFR1 SIGNALING

    doi: 10.1016/j.bbi.2014.04.003

    Figure Lengend Snippet: Analysis of adult neurogenesis in the hippocampus and olfactory bulbs 3 and 12 weeks after injury. A , The number of BrdU + cells was similar between CCI and sham mice but the number of BrdU + /NeuN + cells were dramatically reduced in the GCL of CCI mice

    Article Snippet: For NeuN and BrdU double-labeling, sections were incubated with mouse anti-NeuN primary antibody (1:100; Millipore), followed by anti-mouse Alexa 594-conjugated IgG (1:750, Invitrogen).

    Techniques: Mouse Assay

    Post-acute transplantation of neural progenitor cells (NPCs) yields high neuronal differentiation rates. NPCs were intravenously transplanted at the time points given (i.e., day 0, day 1, and day 28 post stroke) and animals were killed on day 56 or on day 84 followed by immunohistochemical analyses. ( a ) Assessment of GFP + NPCs within the ischemic hemisphere depending on cell delivery time points. ( b ) Differentiation analysis of GFP + NPCs from ( a ) regarding co-expression of Nestin, GFAP, CNPase, Dcx and NeuN. All data are given as mean±S.D. *Significantly different from controls, P

    Journal: Cell Death & Disease

    Article Title: Effects of acute versus post-acute systemic delivery of neural progenitor cells on neurological recovery and brain remodeling after focal cerebral ischemia in mice

    doi: 10.1038/cddis.2014.359

    Figure Lengend Snippet: Post-acute transplantation of neural progenitor cells (NPCs) yields high neuronal differentiation rates. NPCs were intravenously transplanted at the time points given (i.e., day 0, day 1, and day 28 post stroke) and animals were killed on day 56 or on day 84 followed by immunohistochemical analyses. ( a ) Assessment of GFP + NPCs within the ischemic hemisphere depending on cell delivery time points. ( b ) Differentiation analysis of GFP + NPCs from ( a ) regarding co-expression of Nestin, GFAP, CNPase, Dcx and NeuN. All data are given as mean±S.D. *Significantly different from controls, P

    Article Snippet: The following primary antibodies were used: monoclonal mouse anti-BrdU antibody (1 : 400; Roche, Basel, Switzerland), monoclonal rat anti-BrdU antibody (1 : 400; Abcam, Cambridge, UK), polyclonal rabbit anti-GFP antibody (1 : 2500; Abcam; used for intensification of the GFP signal due to quenching effects after tissue processing), goat polyclonal anti-doublecortin (1 : 50; Santa Cruz Biotechnology, Heidelberg, Germany), rat polyclonal anti-GFAP antibody (1 : 500; Zymed, Paisley, UK), mouse monoclonal anti-CNPase (1 : 400; Millipore, Nottingham, UK), a mouse monoclonal anti-NeuN (1 : 1000; Millipore), a rat biotin-conjugated anti-IB4 antibody (1 : 100; Vector Labs, Peterbourough, UK), or mouse monoclonal anti-nestin (1 : 500; Millipore).

    Techniques: Transplantation Assay, Immunohistochemistry, Expressing

    Some pubertally born cells acquire mature neuronal and glial phenotypes. Pie charts represent the percentage of BrdU-ir cells (± SEM) double-labeled with mature neuronal marker NeuN, astrocytic marker GFAP, or neither.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Pubertally born neurons and glia are functionally integrated into limbic and hypothalamic circuits of the male Syrian hamster

    doi: 10.1073/pnas.1219443110

    Figure Lengend Snippet: Some pubertally born cells acquire mature neuronal and glial phenotypes. Pie charts represent the percentage of BrdU-ir cells (± SEM) double-labeled with mature neuronal marker NeuN, astrocytic marker GFAP, or neither.

    Article Snippet: The primary antibody mixture solution contained monoclonal rat anti-BrdU (Serotec) at a working concentration of 1 μg/mL, monoclonal mouse anti-NeuN (MAB377; Chemicon International) at a working concentration of 1 μg/mL, and polyclonal anti-GFAP (catalog no. Z0334; Dako) at a working concentration of 0.58 μg/mL.

    Techniques: Labeling, Marker

    5-bromo-2′-deoxyuridine (BrdU)/neuron-specific nuclear protein (NeuN)-positive cells in the dentate gyrus (immunofluorescent double-label staining, × 10). BrdU-labeled apoptotic cells are red, and the fluorochrome is Texas Red; NeuN-labeled cells are blue, and the fluorochrome is Cy5. There are significantly more BrdU-positive cells in the dentate subgranular zone in the hyperoxia group (A) compared with the N-methyl-D-aspartate receptor 3A knock out group (B) and the control group (C). A few BrdU + /NeuN + cells are detected.

    Journal: Neural Regeneration Research

    Article Title: N-methyl-D-aspartate receptor subtype 3A promotes apoptosis in developing mouse brain exposed to hyperoxia

    doi: 10.3969/j.issn.1673-5374.2012.04.006

    Figure Lengend Snippet: 5-bromo-2′-deoxyuridine (BrdU)/neuron-specific nuclear protein (NeuN)-positive cells in the dentate gyrus (immunofluorescent double-label staining, × 10). BrdU-labeled apoptotic cells are red, and the fluorochrome is Texas Red; NeuN-labeled cells are blue, and the fluorochrome is Cy5. There are significantly more BrdU-positive cells in the dentate subgranular zone in the hyperoxia group (A) compared with the N-methyl-D-aspartate receptor 3A knock out group (B) and the control group (C). A few BrdU + /NeuN + cells are detected.

    Article Snippet: Prior to BrdU staining, the primary antibody used was the mouse anti-NeuN monoclonal antibody (Chemicon; 1: 400) and the secondary antibody was a Cy5 goat anti-mouse monoclonal antibody (Jackson ImmunoResearch; 1: 400).

    Techniques: Staining, Labeling, Knock-Out

    Double immunofluorescence staining with the indicated pairs of antibodies. Nuclei were counterstained with DAPI (blue). p62 (green) and SCMAS (red) immunopositivities were observed in the same cells of IDS-KO mice, confirming SCMAS as a marker of autophagy ( A : e – h ). Double immunofluorescence staining of SCMAS and NeuN (a neuronal marker) or of p62 and NeuN showed positive responses in the same cells ( B , C : e – h ). Double immunofluorescence staining of p62 and platelet-derived growth factor beta receptor (PDGFR-β) (a pericyte marker) showed positive responses in the same cells ( D : e – h ). Double immunofluorescence staining of p62 and iba 1 (a microglial marker) showed positive reactions for both in the same cells ( E : e – h ). Specimens from wild-type mice were immunonegative for p62 and SCMAS ( A – E : a – d ). Bar = 1 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Autophagy in the Central Nervous System and Effects of Chloroquine in Mucopolysaccharidosis Type II Mice

    doi: 10.3390/ijms20235829

    Figure Lengend Snippet: Double immunofluorescence staining with the indicated pairs of antibodies. Nuclei were counterstained with DAPI (blue). p62 (green) and SCMAS (red) immunopositivities were observed in the same cells of IDS-KO mice, confirming SCMAS as a marker of autophagy ( A : e – h ). Double immunofluorescence staining of SCMAS and NeuN (a neuronal marker) or of p62 and NeuN showed positive responses in the same cells ( B , C : e – h ). Double immunofluorescence staining of p62 and platelet-derived growth factor beta receptor (PDGFR-β) (a pericyte marker) showed positive responses in the same cells ( D : e – h ). Double immunofluorescence staining of p62 and iba 1 (a microglial marker) showed positive reactions for both in the same cells ( E : e – h ). Specimens from wild-type mice were immunonegative for p62 and SCMAS ( A – E : a – d ). Bar = 1 μm.

    Article Snippet: Sections were also stained with anti-p62 rat monoclonal antibody (1:200) in combination with anti-NeuN mouse monoclonal antibody (1:100, Millipore), anti-iba-1 rabbit polyclonal antibody (1:400, Wako, Richmond, VA, USA), or anti-PDGFR-β rabbit polyclonal antibody (1:100, Santa Cruz) overnight at 4 °C.

    Techniques: Double Immunofluorescence Staining, Mouse Assay, Marker, Derivative Assay