Journal: Frontiers in Neural Circuits
Article Title: Neurochemical properties of BDNF-containing neurons projecting to rostral ventromedial medulla in the ventrolateral periaqueductal gray
Figure Lengend Snippet: Fluorescent photomicrographs show both neurons and astrocytes express BDNF, but not microglia . (A–D) Colocalization of BDNF (green) and NeuN (red), NeuN is the marker for neurons. (E–H) Colocalization of BDNF (green) and GFAP (red), GFAP is the marker for astrocytes. (I–L) Expression of BDNF (green) and OX42 (red), OX42 is the marker for microglia. The framed areas in (C , G , K) are magnified in (D , H , L) respectively. Arrows indicate the colocalization of BDNF/NeuN (D) and BDNF/GFAP (H) ; arrowheads indicate the single expression of NeuN (D) or OX42 (L) . Scale bars = 20 μm in (K) (applies A–C , E–G , I–K ); 10 μm in (H) (applies D , H , L ).
Article Snippet: The antibodies used in the current study were rabbit anti-BDNF antiserum (ab6201; Abcam, Cambridge, MA, USA); mouse anti-NeuN antiserum (MAB377; Millipore, Billerica, MA, USA); mouse anti-GFAP antiserum (MAB3402; Millipore); mouse anti-OX42 antiserum (CBL1512; Millipore); guinea pig anti-FG antiserum (NM-101; PROTOS BIOTECH CORP, New York, NY, USA); goat anti-5-HT antiserum (20079; ImmunoStar, Houston, Texas, USA); rat anti-NT antiserum (NP-103; PROTOS BIOTECH CORP); rat anti-SP antiserum (MAB356; Millipore); goat anti-CGRP antiserum (ab36001; Abcam); mouse anti-NOS antiserum (N-2280; Sigma, St. Louis, MO, USA); mouse anti-PV antiserum (P-3171; Sigma); mouse anti-TH antiserum (T-2928; Sigma); goat anti-TrkB antiserum (sc-20542; Santa Cruz Biotechnology, Santa Cruz, CA, USA); mouse anti-FOS antiserum (ab11959; Abcam); biotin-donkey anti-rabbit IgG (AP182F; Millipore); Alexa594-donkey anti-mouse IgG (A21203; Invitrogen, Carlsbad, CA, USA); Alexa594-goat anti-guinea pig IgG (A-11076; Invitrogen); Alexa594-donkey anti-goat IgG (A-11058; Invitrogen); Cy3-donkey anti-rat IgG (AP189c; Millipore); Alexa647-donkey anti-guinea pig IgG (AP193SA6; Millipore); FITC-Avidin (A-2001; Vector, Burlingame, CA, USA).
Techniques: Marker, Expressing