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    Sino Biological mouse anti mers cov n iggs
    Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold <t>MERS-CoV</t> suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P
    Mouse Anti Mers Cov N Iggs, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    mouse anti mers cov n iggs - by Bioz Stars, 2021-07
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    97
    Sino Biological sars cov nucleoprotein np antibody rabbit pab
    Kinetics of virus-specific T cell responses in BALF and lung of <t>SARS-CoV-2–infected</t> BALB/c and C57BL/6 mice. (A–D) Lymphocytes from airway and lung of transduced/infected WT BALB/c mice were harvested at indicated time points after infection and stimulated with 5 µM N351 (A and B) and 1 µM S535 (C and D) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers of antigen-specific T cells (right) in BALF (A and C) and lung (B and D) are shown ( n = 3 or 4 mice; data are representative of three independent experiments). (E–H) Lymphocytes from airway and lung of transduced/infected C57BL/6 mice were harvested at indicated time points and stimulated with 5 µM ORF3a 266 (E and F) and 1 µM S538 (G and H) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers (right) of antigen-specific T cells are shown ( n = 3 or 4 mice; data are representative of three independent experiments). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide; p.i., post-infection.
    Sars Cov Nucleoprotein Np Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov nucleoprotein np antibody rabbit pab/product/Sino Biological
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov nucleoprotein np antibody rabbit pab - by Bioz Stars, 2021-07
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      Buy from Supplier

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    Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P

    Journal: Journal of Virology

    Article Title: HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV

    doi: 10.1128/JVI.01622-20

    Figure Lengend Snippet: Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P

    Article Snippet: To visualize MERS-CoV particles, cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (Sino Biological, China) (1:1,000 dilution), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (Thermo Fisher Scientific, Poland) (2.5 μg/ml).

    Techniques: Incubation, Confocal Microscopy, MANN-WHITNEY

    HTCC blocks the interaction between the virus and its entry receptor. Precooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or subjected to mock treatment in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red), and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Colocalization of DPP4 with MERS-CoV-N was determined by confocal microscopy, and the results are presented as Manders’ M2 coefficient values after excluding nonspecific nuclear signal. Colocalization analysis was carried out with ImageJ JACoP (Just Another Colocalization Plugin). The decrease in colocalization was statistically significant ( P

    Journal: Journal of Virology

    Article Title: HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV

    doi: 10.1128/JVI.01622-20

    Figure Lengend Snippet: HTCC blocks the interaction between the virus and its entry receptor. Precooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or subjected to mock treatment in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red), and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Colocalization of DPP4 with MERS-CoV-N was determined by confocal microscopy, and the results are presented as Manders’ M2 coefficient values after excluding nonspecific nuclear signal. Colocalization analysis was carried out with ImageJ JACoP (Just Another Colocalization Plugin). The decrease in colocalization was statistically significant ( P

    Article Snippet: To visualize MERS-CoV particles, cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (Sino Biological, China) (1:1,000 dilution), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (Thermo Fisher Scientific, Poland) (2.5 μg/ml).

    Techniques: Incubation, Confocal Microscopy

    Kinetics of virus-specific T cell responses in BALF and lung of SARS-CoV-2–infected BALB/c and C57BL/6 mice. (A–D) Lymphocytes from airway and lung of transduced/infected WT BALB/c mice were harvested at indicated time points after infection and stimulated with 5 µM N351 (A and B) and 1 µM S535 (C and D) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers of antigen-specific T cells (right) in BALF (A and C) and lung (B and D) are shown ( n = 3 or 4 mice; data are representative of three independent experiments). (E–H) Lymphocytes from airway and lung of transduced/infected C57BL/6 mice were harvested at indicated time points and stimulated with 5 µM ORF3a 266 (E and F) and 1 µM S538 (G and H) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers (right) of antigen-specific T cells are shown ( n = 3 or 4 mice; data are representative of three independent experiments). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide; p.i., post-infection.

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: Kinetics of virus-specific T cell responses in BALF and lung of SARS-CoV-2–infected BALB/c and C57BL/6 mice. (A–D) Lymphocytes from airway and lung of transduced/infected WT BALB/c mice were harvested at indicated time points after infection and stimulated with 5 µM N351 (A and B) and 1 µM S535 (C and D) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers of antigen-specific T cells (right) in BALF (A and C) and lung (B and D) are shown ( n = 3 or 4 mice; data are representative of three independent experiments). (E–H) Lymphocytes from airway and lung of transduced/infected C57BL/6 mice were harvested at indicated time points and stimulated with 5 µM ORF3a 266 (E and F) and 1 µM S538 (G and H) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers (right) of antigen-specific T cells are shown ( n = 3 or 4 mice; data are representative of three independent experiments). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide; p.i., post-infection.

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Infection, Mouse Assay

    Mapping SARS-CoV-2 T cell epitopes in BALB/c mice. (A) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) peptides. Antigen-specific CD4 + T cell responses were determined by intracellular IFN-γ staining. (B) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) peptides. Antigen-specific CD8 + T cell responses were determined. (C) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) and corresponding truncated 13–15-mer peptides for 5–6 h in the presence of brefeldin A. Antigen-specific CD4 + T cell responses were determined. (D) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) and corresponding truncated 8–9-mer peptides for 5–6 h in the presence of brefeldin A. Antigen-specific CD8 + T cell responses were determined. Candidate truncated epitopes are labeled with # ( n = 3; data are representative of at least two independent experiments). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide.

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: Mapping SARS-CoV-2 T cell epitopes in BALB/c mice. (A) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) peptides. Antigen-specific CD4 + T cell responses were determined by intracellular IFN-γ staining. (B) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) peptides. Antigen-specific CD8 + T cell responses were determined. (C) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) and corresponding truncated 13–15-mer peptides for 5–6 h in the presence of brefeldin A. Antigen-specific CD4 + T cell responses were determined. (D) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) and corresponding truncated 8–9-mer peptides for 5–6 h in the presence of brefeldin A. Antigen-specific CD8 + T cell responses were determined. Candidate truncated epitopes are labeled with # ( n = 3; data are representative of at least two independent experiments). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide.

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Mouse Assay, Staining, Labeling

    SARS-CoV-2–specific CD4 + T cells and CD8 + T cells were polyfunctional. (A and E) Phenotyping BALF-derived SARS-CoV-2-N351–specific CD4 + T cells (A) and SARS-CoV-2-S535–specific CD8 + T cells (E). (B and F) Cytokine expression of airway-derived N351-specific CD4 + T cells (B) and S535-specific CD8 + T cells (F) are shown ( n = 3 mice; data are representative of two independent experiments). (C and G) Functional avidity curves (left) of airway- and lung-derived N351-specific CD4 + T cells (C) and S535-specific CD8 + T cells (G) and the amount of peptide required for half-maximum response (EC 50 ) are shown (right; n = 3 mice; data are representative of two independent experiments; Student’s t tests; P value of C is 0.0004; P value of G is 0.0051). (D and H) Representative flow histograms (left) and killing rates (right) of in vivo cytotoxicity of N351-specific CD4 + T cells (D) and S535-specific CD8 + T cells (H) in SARS-CoV-2–infected mice and mock-infected mice are shown ( n = 5 mice per group; data are representative of two independent experiments; Student’s t tests; P value of D is 0.0062; P value of H is 0.0002). *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: SARS-CoV-2–specific CD4 + T cells and CD8 + T cells were polyfunctional. (A and E) Phenotyping BALF-derived SARS-CoV-2-N351–specific CD4 + T cells (A) and SARS-CoV-2-S535–specific CD8 + T cells (E). (B and F) Cytokine expression of airway-derived N351-specific CD4 + T cells (B) and S535-specific CD8 + T cells (F) are shown ( n = 3 mice; data are representative of two independent experiments). (C and G) Functional avidity curves (left) of airway- and lung-derived N351-specific CD4 + T cells (C) and S535-specific CD8 + T cells (G) and the amount of peptide required for half-maximum response (EC 50 ) are shown (right; n = 3 mice; data are representative of two independent experiments; Student’s t tests; P value of C is 0.0004; P value of G is 0.0051). (D and H) Representative flow histograms (left) and killing rates (right) of in vivo cytotoxicity of N351-specific CD4 + T cells (D) and S535-specific CD8 + T cells (H) in SARS-CoV-2–infected mice and mock-infected mice are shown ( n = 5 mice per group; data are representative of two independent experiments; Student’s t tests; P value of D is 0.0062; P value of H is 0.0002). *, P

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Derivative Assay, Expressing, Mouse Assay, Functional Assay, In Vivo, Infection

    Mapping SARS-CoV-2 T cell epitopes in C57BL/6 mice. (A) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) peptides. Antigen-specific CD4 + T cell responses were determined by intracellular IFN-γ staining. (B) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) peptides. Antigen-specific CD8 + T cell responses were determined. (C) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) and corresponding truncated 13–15-mer peptides. Antigen-specific CD4 + T cell responses were determined. (D) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) and corresponding truncated 8–9-mer peptides. Antigen-specific CD8 + T cell responses were determined. Candidate truncated epitopes are labeled with # ( n = 3; data are representative of at least two independent experiments). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide.

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: Mapping SARS-CoV-2 T cell epitopes in C57BL/6 mice. (A) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) peptides. Antigen-specific CD4 + T cell responses were determined by intracellular IFN-γ staining. (B) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) peptides. Antigen-specific CD8 + T cell responses were determined. (C) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) and corresponding truncated 13–15-mer peptides. Antigen-specific CD4 + T cell responses were determined. (D) Lymphocytes from vaccinated lungs were stimulated with 5 µM 20-mer (20 amino acids) and corresponding truncated 8–9-mer peptides. Antigen-specific CD8 + T cell responses were determined. Candidate truncated epitopes are labeled with # ( n = 3; data are representative of at least two independent experiments). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide.

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Mouse Assay, Staining, Labeling

    IFN-I signaling was critical for the generation of robust T cell responses against SARS-CoV-2 infection. (A and B) Frequencies (left) and cell numbers (right) of airway-derived N351-specific CD4 + T cells (A) and S535-specific CD8 + T cells (B) at indicated time points are shown ( n = 3 or 4 mice per time point; data are representative of three independent experiments). (C and D) Airway-derived N351-specific CD4 + T cell responses (C) and S535-specific CD8 + T cell responses (D) in WT and KO BALB/c mice are compared ( n = 3 or 4 mice; data are representative of two independent experiments; Student’s t tests; P values of C are 0.0006 and 0.0013; P values of D are 0.0005 and 0.0029). (E and F) Representative flow plots of N351-specific CD4 + T cells (E) and S535-specific CD8 + T cells (F) are shown (left). Bi-cytokine expression capability (right three panels) is statistically different between WT and KO mice ( n = 3 or 4 mice; data are representative of two indep endent experiments; Student’s t tests; P values of E are 0.0003, 0.0057, and 0.0004; P values of F are

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: IFN-I signaling was critical for the generation of robust T cell responses against SARS-CoV-2 infection. (A and B) Frequencies (left) and cell numbers (right) of airway-derived N351-specific CD4 + T cells (A) and S535-specific CD8 + T cells (B) at indicated time points are shown ( n = 3 or 4 mice per time point; data are representative of three independent experiments). (C and D) Airway-derived N351-specific CD4 + T cell responses (C) and S535-specific CD8 + T cell responses (D) in WT and KO BALB/c mice are compared ( n = 3 or 4 mice; data are representative of two independent experiments; Student’s t tests; P values of C are 0.0006 and 0.0013; P values of D are 0.0005 and 0.0029). (E and F) Representative flow plots of N351-specific CD4 + T cells (E) and S535-specific CD8 + T cells (F) are shown (left). Bi-cytokine expression capability (right three panels) is statistically different between WT and KO mice ( n = 3 or 4 mice; data are representative of two indep endent experiments; Student’s t tests; P values of E are 0.0003, 0.0057, and 0.0004; P values of F are

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Infection, Derivative Assay, Mouse Assay, Expressing

    Identification of CD4 + and CD8 + T cell epitopes in SARS-CoV-2–infected WT BALB/c and C57BL/6 mice. (A and B) Confirmation of CD4 + T cell epitopes (A) and CD8 + T cell epitopes (B) in infected BALB/c mice. Flow plots and summary columns are shown ( n = 3; data verified in two independent experiments). (C and D) Confirmation of CD4 + T cell epitopes (C) and CD8 + T cell epitopes (D) in infected C57BL/6 mice. Flow plots and summary columns are shown ( n = 3; data verified in two independent experiments). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide.

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: Identification of CD4 + and CD8 + T cell epitopes in SARS-CoV-2–infected WT BALB/c and C57BL/6 mice. (A and B) Confirmation of CD4 + T cell epitopes (A) and CD8 + T cell epitopes (B) in infected BALB/c mice. Flow plots and summary columns are shown ( n = 3; data verified in two independent experiments). (C and D) Confirmation of CD4 + T cell epitopes (C) and CD8 + T cell epitopes (D) in infected C57BL/6 mice. Flow plots and summary columns are shown ( n = 3; data verified in two independent experiments). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide.

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Infection, Mouse Assay

    Kinetics of virus-specific T cell responses in DLNs and spleens of SARS-CoV-2–infected BALB/c and C57BL/6 mice. (A–D) Lymphocytes from DLN and spleen of transduced/infected WT BALB/c mice were harvested at indicated time points after infection and stimulated with 5 µM N351 (A and B) and 1 µM S535 (C and D) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers of antigen-specific T cells (right) in DLN (A and C) and spleen (B and D) are shown ( n = 3 mice; data are representative of one experiment). (E–H) Lymphocytes from DLN and spleen of transduced/infected C57BL/6 mice were harvested at indicated time points and stimulated with 5 µM ORF3a 266 (E and F) and 1 µM S538 (G and H) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers (right) of antigen-specific T cells are shown ( n = 3; data are representative of one experiment). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide; p.i., post-infection.

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: Kinetics of virus-specific T cell responses in DLNs and spleens of SARS-CoV-2–infected BALB/c and C57BL/6 mice. (A–D) Lymphocytes from DLN and spleen of transduced/infected WT BALB/c mice were harvested at indicated time points after infection and stimulated with 5 µM N351 (A and B) and 1 µM S535 (C and D) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers of antigen-specific T cells (right) in DLN (A and C) and spleen (B and D) are shown ( n = 3 mice; data are representative of one experiment). (E–H) Lymphocytes from DLN and spleen of transduced/infected C57BL/6 mice were harvested at indicated time points and stimulated with 5 µM ORF3a 266 (E and F) and 1 µM S538 (G and H) for 6 h in the presence of brefeldin A. The frequencies (left) and cell numbers (right) of antigen-specific T cells are shown ( n = 3; data are representative of one experiment). All results are expressed as mean ± SEM. Ag, antigen; pep, peptide; p.i., post-infection.

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Infection, Mouse Assay

    Epitope-specific CD4 + and CD8 + T cells partially protected SARS-CoV-2–infected mice from severe disease. (A) Strategy of VRP vaccination and SARS-CoV-2 challenge. (B and C) Effects of N351-specific CD4 + T cells (B) and S535-specific CD8 + T cells (C) in BALB/c mice. Cell numbers of airway-derived antigen-specific T cells are shown ( n = 3 or 4 mice per group per time point; left). Viral titers in the lungs were measured at the indicated time points ( n = 4 mice per group per time point; middle; data are representative of at least two independent experiments; Student’s t tests; P values of B are 0.0051 and 0.0403; P values of C are 0.0026 and 0.0804). Sections of paraffin-embedded lungs from infected mice at 4 d.p.i. were stained with hematoxylin/eosin ( n = 3 mice per group per time point; right; data are representative of at least two independent experiments). Scale bar, 100 mm. (D) Effects of S538-specific CD8 + T cells in C57BL/6 mice. Cell numbers at indicated time points are shown ( n = 3 mice per group per time point; left). Viral titers in the lungs were measured at the indicated time points ( n = 4 mice per group per time point; middle; data are representative of at least two independent experiments; Student’s t tests; P values of D are 0.0372 and 0.0043). Sections of paraffin-embedded lungs from infected mice at 6 d after infection were stained with hematoxylin/eosin ( n = 3 mice per group per time point; right; data are representative of at least two independent experiments). Scale bar, 100 mm. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: Epitope-specific CD4 + and CD8 + T cells partially protected SARS-CoV-2–infected mice from severe disease. (A) Strategy of VRP vaccination and SARS-CoV-2 challenge. (B and C) Effects of N351-specific CD4 + T cells (B) and S535-specific CD8 + T cells (C) in BALB/c mice. Cell numbers of airway-derived antigen-specific T cells are shown ( n = 3 or 4 mice per group per time point; left). Viral titers in the lungs were measured at the indicated time points ( n = 4 mice per group per time point; middle; data are representative of at least two independent experiments; Student’s t tests; P values of B are 0.0051 and 0.0403; P values of C are 0.0026 and 0.0804). Sections of paraffin-embedded lungs from infected mice at 4 d.p.i. were stained with hematoxylin/eosin ( n = 3 mice per group per time point; right; data are representative of at least two independent experiments). Scale bar, 100 mm. (D) Effects of S538-specific CD8 + T cells in C57BL/6 mice. Cell numbers at indicated time points are shown ( n = 3 mice per group per time point; left). Viral titers in the lungs were measured at the indicated time points ( n = 4 mice per group per time point; middle; data are representative of at least two independent experiments; Student’s t tests; P values of D are 0.0372 and 0.0043). Sections of paraffin-embedded lungs from infected mice at 6 d after infection were stained with hematoxylin/eosin ( n = 3 mice per group per time point; right; data are representative of at least two independent experiments). Scale bar, 100 mm. *, P

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Infection, Mouse Assay, Derivative Assay, Staining

    SARS-CoV-2–specific CD8 + T cells were polyfunctional in infected C57BL/6 mice. (A) Cells from BALF were stained with antibodies against the indicated markers. Histograms shown here were gated on SARS-CoV-2-S538–specific CD8 + T cells. (B) Cytokine expression of airway derived SARS-CoV-2-S538–specific CD8 + T cells are shown ( n = 3 or 4 mice; data are representative of one experiment). (C) Functional avidity curves (left) of airway- and lung-derived SARS-CoV-2-S538–specific CD8 + T cells and the amount of peptide required for half-maximum response (EC 50 ) are shown (right; n = 3; data are representative of two independent experiments; Student’s t tests; P value of C is 0.011). (D) Representative flow histograms (left) and killing rates (right) of in vivo cytotoxicity of SARS-CoV-2-S538–specific CD8 + T cells in SARS-CoV-2–infected mice and mock-infected mice are shown ( n = 5; data are representative of one experiment; Student’s t tests; P value of C is

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: SARS-CoV-2–specific CD8 + T cells were polyfunctional in infected C57BL/6 mice. (A) Cells from BALF were stained with antibodies against the indicated markers. Histograms shown here were gated on SARS-CoV-2-S538–specific CD8 + T cells. (B) Cytokine expression of airway derived SARS-CoV-2-S538–specific CD8 + T cells are shown ( n = 3 or 4 mice; data are representative of one experiment). (C) Functional avidity curves (left) of airway- and lung-derived SARS-CoV-2-S538–specific CD8 + T cells and the amount of peptide required for half-maximum response (EC 50 ) are shown (right; n = 3; data are representative of two independent experiments; Student’s t tests; P value of C is 0.011). (D) Representative flow histograms (left) and killing rates (right) of in vivo cytotoxicity of SARS-CoV-2-S538–specific CD8 + T cells in SARS-CoV-2–infected mice and mock-infected mice are shown ( n = 5; data are representative of one experiment; Student’s t tests; P value of C is

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Infection, Mouse Assay, Staining, Expressing, Derivative Assay, Functional Assay, In Vivo

    Cross-reactive T cell responses were found between SARS-CoV-2 and SARS-CoV in SARS-CoV-2–infected mice. (A) Characteristics of conserved T cell epitopes in SARS-CoV-2, SARS-CoV, and MERS-CoV. (B and C) BALB/c mice were transduced and infected with SARS-CoV-2. Lymphocytes derived from airway were prepared at 8 d.p.i. and stimulated with conversed epitopes. CD4 + (B) and CD8 + T cell responses (C) were detected by IFN-γ expression. Flow plots (left) and cross-reactivity rate (right) are shown ( n = 3 or 4 mice per group; data are representative of two independent experiments). (D) Functional avidity curves (left) of S535-specific CD8 + T cells and S521–cross-reactive CD8 + T cells and the amount of peptide required for half-maximum response (EC 50 ) are shown (right; n = 3 mice; data are representative of two independent experiments; Student’s t tests; P value of D is 0.0182). *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Mapping and role of T cell response in SARS-CoV-2–infected mice

    doi: 10.1084/jem.20202187

    Figure Lengend Snippet: Cross-reactive T cell responses were found between SARS-CoV-2 and SARS-CoV in SARS-CoV-2–infected mice. (A) Characteristics of conserved T cell epitopes in SARS-CoV-2, SARS-CoV, and MERS-CoV. (B and C) BALB/c mice were transduced and infected with SARS-CoV-2. Lymphocytes derived from airway were prepared at 8 d.p.i. and stimulated with conversed epitopes. CD4 + (B) and CD8 + T cell responses (C) were detected by IFN-γ expression. Flow plots (left) and cross-reactivity rate (right) are shown ( n = 3 or 4 mice per group; data are representative of two independent experiments). (D) Functional avidity curves (left) of S535-specific CD8 + T cells and S521–cross-reactive CD8 + T cells and the amount of peptide required for half-maximum response (EC 50 ) are shown (right; n = 3 mice; data are representative of two independent experiments; Student’s t tests; P value of D is 0.0182). *, P

    Article Snippet: Cells were then incubated with a rabbit anti–SARS-CoV N protein polyclonal antibody (40143-T62; Sino Biological, Inc.) followed by an HRP-labeled goat anti-rabbit secondary antibody (109–035-088; Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Infection, Mouse Assay, Derivative Assay, Expressing, Functional Assay