mouse anti gapdh antibody Search Results


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    Cell Signaling Technology Inc anti gapdh
    Anti Gapdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gapdh/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    anti gapdh - by Bioz Stars, 2022-08
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    99
    Cell Signaling Technology Inc rabbit anti gapdh
    Rabbit Anti Gapdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gapdh/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    rabbit anti gapdh - by Bioz Stars, 2022-08
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    97
    Millipore mouse anti gapdh
    Mouse Anti Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti gapdh/product/Millipore
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    86
    Cell Signaling Technology Inc anti gapdh antibody
    <t>HDAC4</t> is the target gene of miRNA-200b-3p. a Identification the binding sites of hsa-miR-200b-3p in the HDAC4 3′-UTR (Red). b HUVECs were transfect with NC mimic and miR-200b mimic for 24 h, the mRNA level of HDAC4 in HUVECs were examined by qRT-PCR, and normalized with <t>GAPDH.</t> (n = 4 for each group). c The protein level HDAC4 in HUVECs was examined by western blot, and normalized with β-Actin (n = 3 for each group). Full-length blots/gels are presented in Additional file 4 : Figure S3. d HUVECs were transfect with NC/miR-200b mimic with control adenovirus (Con-Ad) or HDAC4 over-expression adenovirus (HDAC4-Ad) for 48 h, the expression of HDAC4 was accessed by qRT-PCR (n = 4 for each group). e Con-Ad or HDAC4-Ad transfected HUVECs were stimulated with ox-LDL for 6 h. The apoptosis rate of HUVECs was accessed by Annexin V and PI staining (n = 4 for each group). Unpaired t test was used for comparison between groups, * p
    Anti Gapdh Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gapdh antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gapdh antibody - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

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    HDAC4 is the target gene of miRNA-200b-3p. a Identification the binding sites of hsa-miR-200b-3p in the HDAC4 3′-UTR (Red). b HUVECs were transfect with NC mimic and miR-200b mimic for 24 h, the mRNA level of HDAC4 in HUVECs were examined by qRT-PCR, and normalized with GAPDH. (n = 4 for each group). c The protein level HDAC4 in HUVECs was examined by western blot, and normalized with β-Actin (n = 3 for each group). Full-length blots/gels are presented in Additional file 4 : Figure S3. d HUVECs were transfect with NC/miR-200b mimic with control adenovirus (Con-Ad) or HDAC4 over-expression adenovirus (HDAC4-Ad) for 48 h, the expression of HDAC4 was accessed by qRT-PCR (n = 4 for each group). e Con-Ad or HDAC4-Ad transfected HUVECs were stimulated with ox-LDL for 6 h. The apoptosis rate of HUVECs was accessed by Annexin V and PI staining (n = 4 for each group). Unpaired t test was used for comparison between groups, * p

    Journal: BMC Cardiovascular Disorders

    Article Title: MicroRNA-200b-3p promotes endothelial cell apoptosis by targeting HDAC4 in atherosclerosis

    doi: 10.1186/s12872-021-01980-0

    Figure Lengend Snippet: HDAC4 is the target gene of miRNA-200b-3p. a Identification the binding sites of hsa-miR-200b-3p in the HDAC4 3′-UTR (Red). b HUVECs were transfect with NC mimic and miR-200b mimic for 24 h, the mRNA level of HDAC4 in HUVECs were examined by qRT-PCR, and normalized with GAPDH. (n = 4 for each group). c The protein level HDAC4 in HUVECs was examined by western blot, and normalized with β-Actin (n = 3 for each group). Full-length blots/gels are presented in Additional file 4 : Figure S3. d HUVECs were transfect with NC/miR-200b mimic with control adenovirus (Con-Ad) or HDAC4 over-expression adenovirus (HDAC4-Ad) for 48 h, the expression of HDAC4 was accessed by qRT-PCR (n = 4 for each group). e Con-Ad or HDAC4-Ad transfected HUVECs were stimulated with ox-LDL for 6 h. The apoptosis rate of HUVECs was accessed by Annexin V and PI staining (n = 4 for each group). Unpaired t test was used for comparison between groups, * p

    Article Snippet: Membranes were incubated with specific primary antibodies [anti-BCL2 antibody (Rabbit, 1:1000 dilution), anti-BAX antibody (Rabbit, 1:1000 dilution), anti-HDAC4 antibody (Rabbit, 1:1000 dilution), anti-GAPDH antibody (mouse, 1:1000 dilution) and anti-β-Actin antibody (mouse, 1:1000 dilution), from Cell Signaling Technology] at 4 °C overnight.

    Techniques: Binding Assay, Quantitative RT-PCR, Western Blot, Over Expression, Expressing, Transfection, Staining

    Overexpression of hsa-miR-200b-3p exacerbates cell apoptosis induced by H 2 O 2 and ox-LDL. a HUVECs were transfected with negative control (NC) mimic (50 nM) and hsa-miR-200b-3p (miR-200b) mimic (50 nM) for 24 h, then qRT-PCR for hsa-miR-200b-3p was used to access the transfection efficiency (n = 4 for each group). b HUVECs were transfected with NC mimic and miR-200b mimic for 24 h, then cells were stimulated with H 2 O 2 (50 μM) or ox-LDL (100 μg/ml) for 6 h. The apoptosis of HUVECs was detected by Annexin V and PI staining. c The apoptosis ratio of HUVECs was calculated (n = 3 for each group). d The mRNA levels of anti-apoptosis gene BCL2 and pro-apoptosis gene BAX in H 2 O 2 stimulated HUVECs were examined by qRT-PCR (n = 4 for each group). e The mRNA levels of BCL2 in ox-LDL stimulated HUVECs were examined by qRT-PCR (n = 4 for each group). f The protein levels of BCL2 and BAX in ox-LDL stimulated HUVECs were examined by western blot, and normalized with GAPDH. The right histogram was the ratio of BCL2 and BAX (n = 3 for each group). Full-length blots/gels are presented in Additional files 2 and 3 : Figure S1 and S2. g The activity of Caspase3/7 in H 2 O 2 stimulated HUVECs was examined by Caspase-Glo® 3/7 Assay System (n = 6 for each group). h HUVECs were transfect with NC mimic and miR-200 mimic for 24 h, then the proliferation rate of HUVECs was accessed by EdU staining (Bar, 50 μm, n = 6 for each group). Unpaired t test was used for comparison between groups, * p

    Journal: BMC Cardiovascular Disorders

    Article Title: MicroRNA-200b-3p promotes endothelial cell apoptosis by targeting HDAC4 in atherosclerosis

    doi: 10.1186/s12872-021-01980-0

    Figure Lengend Snippet: Overexpression of hsa-miR-200b-3p exacerbates cell apoptosis induced by H 2 O 2 and ox-LDL. a HUVECs were transfected with negative control (NC) mimic (50 nM) and hsa-miR-200b-3p (miR-200b) mimic (50 nM) for 24 h, then qRT-PCR for hsa-miR-200b-3p was used to access the transfection efficiency (n = 4 for each group). b HUVECs were transfected with NC mimic and miR-200b mimic for 24 h, then cells were stimulated with H 2 O 2 (50 μM) or ox-LDL (100 μg/ml) for 6 h. The apoptosis of HUVECs was detected by Annexin V and PI staining. c The apoptosis ratio of HUVECs was calculated (n = 3 for each group). d The mRNA levels of anti-apoptosis gene BCL2 and pro-apoptosis gene BAX in H 2 O 2 stimulated HUVECs were examined by qRT-PCR (n = 4 for each group). e The mRNA levels of BCL2 in ox-LDL stimulated HUVECs were examined by qRT-PCR (n = 4 for each group). f The protein levels of BCL2 and BAX in ox-LDL stimulated HUVECs were examined by western blot, and normalized with GAPDH. The right histogram was the ratio of BCL2 and BAX (n = 3 for each group). Full-length blots/gels are presented in Additional files 2 and 3 : Figure S1 and S2. g The activity of Caspase3/7 in H 2 O 2 stimulated HUVECs was examined by Caspase-Glo® 3/7 Assay System (n = 6 for each group). h HUVECs were transfect with NC mimic and miR-200 mimic for 24 h, then the proliferation rate of HUVECs was accessed by EdU staining (Bar, 50 μm, n = 6 for each group). Unpaired t test was used for comparison between groups, * p

    Article Snippet: Membranes were incubated with specific primary antibodies [anti-BCL2 antibody (Rabbit, 1:1000 dilution), anti-BAX antibody (Rabbit, 1:1000 dilution), anti-HDAC4 antibody (Rabbit, 1:1000 dilution), anti-GAPDH antibody (mouse, 1:1000 dilution) and anti-β-Actin antibody (mouse, 1:1000 dilution), from Cell Signaling Technology] at 4 °C overnight.

    Techniques: Over Expression, Transfection, Negative Control, Quantitative RT-PCR, Staining, Western Blot, Activity Assay, Caspase-Glo Assay