mononuclear cells mncs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore mononuclear cells mncs
    Mononuclear Cells Mncs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/Millipore
    Average 99 stars, based on 470 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    91
    Baxter Healthcare mononuclear cells mncs
    CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing <t>CD34</t> + cells with <t>MNCs</t> and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).
    Mononuclear Cells Mncs, supplied by Baxter Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/Baxter Healthcare
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    Lonza mononuclear cells mncs
    CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing <t>CD34</t> + cells with <t>MNCs</t> and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).
    Mononuclear Cells Mncs, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/Lonza
    Average 92 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    AllCells LLC mononuclear cells mncs
    CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing <t>CD34</t> + cells with <t>MNCs</t> and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).
    Mononuclear Cells Mncs, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/AllCells LLC
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    99
    Millipore mononuclear cells mnc
    CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing <t>CD34</t> + cells with <t>MNCs</t> and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).
    Mononuclear Cells Mnc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mnc/product/Millipore
    Average 99 stars, based on 240 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mnc - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    93
    STEMCELL Technologies Inc viable mononuclear cells mncs
    Scheme for generating the humanized NSG mice. <t>MNCs</t> isolated from hUCB were enriched using a <t>RosetteSep</t> kit and human CD34 MicroBead Kit. NSG mice were conditioned by busulfan and CD34 + cells were injected via retro-orbital sinus.
    Viable Mononuclear Cells Mncs, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viable mononuclear cells mncs/product/STEMCELL Technologies Inc
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    viable mononuclear cells mncs - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    99
    Millipore characterization mononuclear cells mncs
    Scheme for generating the humanized NSG mice. <t>MNCs</t> isolated from hUCB were enriched using a <t>RosetteSep</t> kit and human CD34 MicroBead Kit. NSG mice were conditioned by busulfan and CD34 + cells were injected via retro-orbital sinus.
    Characterization Mononuclear Cells Mncs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/characterization mononuclear cells mncs/product/Millipore
    Average 99 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    characterization mononuclear cells mncs - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Biochrom mononuclear cells mononuclear cells mnc
    Scheme for generating the humanized NSG mice. <t>MNCs</t> isolated from hUCB were enriched using a <t>RosetteSep</t> kit and human CD34 MicroBead Kit. NSG mice were conditioned by busulfan and CD34 + cells were injected via retro-orbital sinus.
    Mononuclear Cells Mononuclear Cells Mnc, supplied by Biochrom, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mononuclear cells mnc/product/Biochrom
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mononuclear cells mnc - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    85
    R&D Systems mononuclear cells mnc cd45
    Scheme for generating the humanized NSG mice. <t>MNCs</t> isolated from hUCB were enriched using a <t>RosetteSep</t> kit and human CD34 MicroBead Kit. NSG mice were conditioned by busulfan and CD34 + cells were injected via retro-orbital sinus.
    Mononuclear Cells Mnc Cd45, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mnc cd45/product/R&D Systems
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mnc cd45 - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    88
    GE Healthcare mononuclear cells mnc mononuclear cells
    C7 augmented expansion of rare CD34 + CD90 + CD49 + hematopoietic stem and progenitor cells when cultures were initiated with non‐enriched umbilical cord blood <t>(UCB)–mononucleated</t> cells <t>(MNC).</t> (A): Representative flow cytometer dot plots depicting (a) CD90 + CD49f – (region depicted with *); (b) CD90 + CD49f + (region depicted with **) and (c) CD90 – CD49f + (region depicted with ***) population which are subsets of CD45 + CD34 + CD38 – CD45RA – cells of (i) thawed UCB MNC at 0 hours followed by culturing for 10 days in (ii) cytokine control and (iii) 5.0 µM of C7 supplemented with cytokines using serum free expansion media (SFEM). Media, cytokines, and C7 were replenished at day 7. (B): Expansion of viable (7AAD – ) HSC1 and HSC2 with phenotypic expression of CD45 + CD34 + CD38 – CD45RA – CD90 + CD49f – and CD45 + CD34 + CD38 – CD45RA – CD90 + CD49f + respectively when cultures were initiated with non‐selected UCB–MNC. The expansion cultures lasted for 10 days, with SFEM, cytokine, and 5.0 µM of C7 replenishment being done on day 7. Cytokines alone in media served as the blank control. *, p
    Mononuclear Cells Mnc Mononuclear Cells, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mnc mononuclear cells/product/GE Healthcare
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mnc mononuclear cells - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    92
    Axis-Shield Diagnostics mononuclear cells mncs
    C7 augmented expansion of rare CD34 + CD90 + CD49 + hematopoietic stem and progenitor cells when cultures were initiated with non‐enriched umbilical cord blood <t>(UCB)–mononucleated</t> cells <t>(MNC).</t> (A): Representative flow cytometer dot plots depicting (a) CD90 + CD49f – (region depicted with *); (b) CD90 + CD49f + (region depicted with **) and (c) CD90 – CD49f + (region depicted with ***) population which are subsets of CD45 + CD34 + CD38 – CD45RA – cells of (i) thawed UCB MNC at 0 hours followed by culturing for 10 days in (ii) cytokine control and (iii) 5.0 µM of C7 supplemented with cytokines using serum free expansion media (SFEM). Media, cytokines, and C7 were replenished at day 7. (B): Expansion of viable (7AAD – ) HSC1 and HSC2 with phenotypic expression of CD45 + CD34 + CD38 – CD45RA – CD90 + CD49f – and CD45 + CD34 + CD38 – CD45RA – CD90 + CD49f + respectively when cultures were initiated with non‐selected UCB–MNC. The expansion cultures lasted for 10 days, with SFEM, cytokine, and 5.0 µM of C7 replenishment being done on day 7. Cytokines alone in media served as the blank control. *, p
    Mononuclear Cells Mncs, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/Axis-Shield Diagnostics
    Average 92 stars, based on 180 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    91
    Nycomed mononuclear cells mnc
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mnc, supplied by Nycomed, used in various techniques. Bioz Stars score: 91/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mnc/product/Nycomed
    Average 91 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mnc - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    Ficoll-Paque Pharmacia mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mncs, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/Ficoll-Paque Pharmacia
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Merck KGaA mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mncs, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/Merck KGaA
    Average 92 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing CD34 + cells with MNCs and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).

    Journal: Circulation research

    Article Title: Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb

    doi: 10.1161/CIRCRESAHA.116.310557

    Figure Lengend Snippet: CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing CD34 + cells with MNCs and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).

    Article Snippet: CD34+ cells and the CD34+ -cell–depleted mononuclear cells (MNCs) were obtained from Baxter Healthcare Corporation (Deerfield, IL, USA).

    Techniques: Protein Array, Expressing

    Scheme for generating the humanized NSG mice. MNCs isolated from hUCB were enriched using a RosetteSep kit and human CD34 MicroBead Kit. NSG mice were conditioned by busulfan and CD34 + cells were injected via retro-orbital sinus.

    Journal: Blood research

    Article Title: Humanizing NOD/SCID/IL-2Rγnull (NSG) mice using busulfan and retro-orbital injection of umbilical cord blood-derived CD34+ cells

    doi: 10.5045/br.2016.51.1.31

    Figure Lengend Snippet: Scheme for generating the humanized NSG mice. MNCs isolated from hUCB were enriched using a RosetteSep kit and human CD34 MicroBead Kit. NSG mice were conditioned by busulfan and CD34 + cells were injected via retro-orbital sinus.

    Article Snippet: Mononuclear cells (MNCs) were enriched using a RosetteSep Human Progenitor Enrichment kit (StemCell Technologies, Canada) and isolated from hUCB using Ficoll-Paque PREMIUM (GE Healthcare Bio-Sciences AB, Sweden) density gradient centrifugation.

    Techniques: Mouse Assay, Isolation, Injection

    C7 augmented expansion of rare CD34 + CD90 + CD49 + hematopoietic stem and progenitor cells when cultures were initiated with non‐enriched umbilical cord blood (UCB)–mononucleated cells (MNC). (A): Representative flow cytometer dot plots depicting (a) CD90 + CD49f – (region depicted with *); (b) CD90 + CD49f + (region depicted with **) and (c) CD90 – CD49f + (region depicted with ***) population which are subsets of CD45 + CD34 + CD38 – CD45RA – cells of (i) thawed UCB MNC at 0 hours followed by culturing for 10 days in (ii) cytokine control and (iii) 5.0 µM of C7 supplemented with cytokines using serum free expansion media (SFEM). Media, cytokines, and C7 were replenished at day 7. (B): Expansion of viable (7AAD – ) HSC1 and HSC2 with phenotypic expression of CD45 + CD34 + CD38 – CD45RA – CD90 + CD49f – and CD45 + CD34 + CD38 – CD45RA – CD90 + CD49f + respectively when cultures were initiated with non‐selected UCB–MNC. The expansion cultures lasted for 10 days, with SFEM, cytokine, and 5.0 µM of C7 replenishment being done on day 7. Cytokines alone in media served as the blank control. *, p

    Journal: Stem Cells Translational Medicine

    Article Title: Ex Vivo Expansion of CD34+CD90+CD49f+ Hematopoietic Stem and Progenitor Cells from Non‐Enriched Umbilical Cord Blood with Azole Compounds

    doi: 10.1002/sctm.17-0251

    Figure Lengend Snippet: C7 augmented expansion of rare CD34 + CD90 + CD49 + hematopoietic stem and progenitor cells when cultures were initiated with non‐enriched umbilical cord blood (UCB)–mononucleated cells (MNC). (A): Representative flow cytometer dot plots depicting (a) CD90 + CD49f – (region depicted with *); (b) CD90 + CD49f + (region depicted with **) and (c) CD90 – CD49f + (region depicted with ***) population which are subsets of CD45 + CD34 + CD38 – CD45RA – cells of (i) thawed UCB MNC at 0 hours followed by culturing for 10 days in (ii) cytokine control and (iii) 5.0 µM of C7 supplemented with cytokines using serum free expansion media (SFEM). Media, cytokines, and C7 were replenished at day 7. (B): Expansion of viable (7AAD – ) HSC1 and HSC2 with phenotypic expression of CD45 + CD34 + CD38 – CD45RA – CD90 + CD49f – and CD45 + CD34 + CD38 – CD45RA – CD90 + CD49f + respectively when cultures were initiated with non‐selected UCB–MNC. The expansion cultures lasted for 10 days, with SFEM, cytokine, and 5.0 µM of C7 replenishment being done on day 7. Cytokines alone in media served as the blank control. *, p

    Article Snippet: As described previously, mononuclear cells (MNC) were isolated from the fresh UCB by density gradient centrifugation using Ficoll‐Histopaque Premium (GE Healthcare, U.K.) .

    Techniques: Flow Cytometry, Cytometry, Expressing

    Long‐term multilineage reconstitution of human cells in the bone marrow (BM) of NSG mice transplanted with UCB–MNC grafts expanded in presence of C7. (A): Human CD45 chimerism in BM of female NSG mice at week 19 post‐transplantation. The absolute cell dose of non‐expanded graft was either 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg while the expanded grafts (either fresh or frozen‐thawed) were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg. The scatter plot represents the human CD45 chimerism of individual animals and depicts the geometric mean with 95% CI of respective treatments. No statistical significance was observed between experimental groups. (B): The proportion of progenitor cells present among the total human cells in BM of male and female NSG mice at week 19 post‐transplantation. The absolute cell dose of non‐expanded graft was either 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg. The scatter plot represents the common progenitors (CD45 + CD34 + ), myeloid (CD13 + CD33 + ), and lymphoid (CD45 + CD7 + ) progenitors of individual animals and depicts the geometric mean with 95% CI of respective treatments. No statistical significance was observed between non‐expanded and C7 expanded grafts. (C): The proportion of myeloid cells present among the total human cells in BM of male and female NSG mice at week 19 post‐transplantation. The absolute cell dose of non‐expanded graft was either 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg. The scatter plot represents the monocytes (CD45 + CD33 + ), granulocytes (CD45 + CD13 + /CD15 + /CD66b + ), and megakaryocytes (CD45 + CD41a + ) of individual animals and depicts the geometric mean with 95% CI of respective treatments. No statistical significance was observed between non‐expanded and C7 expanded grafts. (D): The proportion of lymphoid cells present among the total human cells in BM of male and female NSG mice at week 19 post‐transplantation. The absolute cell dose of non‐expanded graft was either 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg. The scatter plot represents the T helper cells (CD45 + CD3 + CD4 + ), cytotoxic T cells (CD45 + CD3 + CD8 + ), B cells (CD45 + CD19 + ), and NK cells (CD45 + CD56 + ) progenitors of individual animals and depicts the geometric mean with 95% CI of respective treatments. No statistical significance was observed between non‐expanded and C7 expanded grafts. (E): Colony forming unit (GM) assay of purified human CD45 + cells from BM of NSG mice transplanted with (i) non‐expanded UCB–MNC; (ii) cytokine expanded UBC–MNC; and (iii) C7 and cytokine expanded UCB–MNC. *, p

    Journal: Stem Cells Translational Medicine

    Article Title: Ex Vivo Expansion of CD34+CD90+CD49f+ Hematopoietic Stem and Progenitor Cells from Non‐Enriched Umbilical Cord Blood with Azole Compounds

    doi: 10.1002/sctm.17-0251

    Figure Lengend Snippet: Long‐term multilineage reconstitution of human cells in the bone marrow (BM) of NSG mice transplanted with UCB–MNC grafts expanded in presence of C7. (A): Human CD45 chimerism in BM of female NSG mice at week 19 post‐transplantation. The absolute cell dose of non‐expanded graft was either 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg while the expanded grafts (either fresh or frozen‐thawed) were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg. The scatter plot represents the human CD45 chimerism of individual animals and depicts the geometric mean with 95% CI of respective treatments. No statistical significance was observed between experimental groups. (B): The proportion of progenitor cells present among the total human cells in BM of male and female NSG mice at week 19 post‐transplantation. The absolute cell dose of non‐expanded graft was either 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg. The scatter plot represents the common progenitors (CD45 + CD34 + ), myeloid (CD13 + CD33 + ), and lymphoid (CD45 + CD7 + ) progenitors of individual animals and depicts the geometric mean with 95% CI of respective treatments. No statistical significance was observed between non‐expanded and C7 expanded grafts. (C): The proportion of myeloid cells present among the total human cells in BM of male and female NSG mice at week 19 post‐transplantation. The absolute cell dose of non‐expanded graft was either 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg. The scatter plot represents the monocytes (CD45 + CD33 + ), granulocytes (CD45 + CD13 + /CD15 + /CD66b + ), and megakaryocytes (CD45 + CD41a + ) of individual animals and depicts the geometric mean with 95% CI of respective treatments. No statistical significance was observed between non‐expanded and C7 expanded grafts. (D): The proportion of lymphoid cells present among the total human cells in BM of male and female NSG mice at week 19 post‐transplantation. The absolute cell dose of non‐expanded graft was either 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg or 5.0 × 10 7 cells/kg. The scatter plot represents the T helper cells (CD45 + CD3 + CD4 + ), cytotoxic T cells (CD45 + CD3 + CD8 + ), B cells (CD45 + CD19 + ), and NK cells (CD45 + CD56 + ) progenitors of individual animals and depicts the geometric mean with 95% CI of respective treatments. No statistical significance was observed between non‐expanded and C7 expanded grafts. (E): Colony forming unit (GM) assay of purified human CD45 + cells from BM of NSG mice transplanted with (i) non‐expanded UCB–MNC; (ii) cytokine expanded UBC–MNC; and (iii) C7 and cytokine expanded UCB–MNC. *, p

    Article Snippet: As described previously, mononuclear cells (MNC) were isolated from the fresh UCB by density gradient centrifugation using Ficoll‐Histopaque Premium (GE Healthcare, U.K.) .

    Techniques: Mouse Assay, Transplantation Assay, Purification

    Identification of a novel small molecule, C7 that is a structural analog of the p38‐MAPK inhibitor SB203580, which could expand HPC from non‐enriched UCB–MNC. (A): Fold expansion of viable (7AAD – ) CD45 + CD34 + CD38 – CD45RA – HPC and TNC in cultures that lasted for 11 days with animal component free media (ACF) media, cytokine, and respective small molecule being replenished at day 7. SB203580, dimethyl sulfoxide, and cytokines alone in ACF media served as the reference compound, vehicle and blank control, respectively. The dashed black and blue line represents > 2.0‐fold expansion of HPC and TNC, respectively compared to cytokine control. Expansion data of HPC and TNC for lead compound C7 is highlighted with red circles. *, p

    Journal: Stem Cells Translational Medicine

    Article Title: Ex Vivo Expansion of CD34+CD90+CD49f+ Hematopoietic Stem and Progenitor Cells from Non‐Enriched Umbilical Cord Blood with Azole Compounds

    doi: 10.1002/sctm.17-0251

    Figure Lengend Snippet: Identification of a novel small molecule, C7 that is a structural analog of the p38‐MAPK inhibitor SB203580, which could expand HPC from non‐enriched UCB–MNC. (A): Fold expansion of viable (7AAD – ) CD45 + CD34 + CD38 – CD45RA – HPC and TNC in cultures that lasted for 11 days with animal component free media (ACF) media, cytokine, and respective small molecule being replenished at day 7. SB203580, dimethyl sulfoxide, and cytokines alone in ACF media served as the reference compound, vehicle and blank control, respectively. The dashed black and blue line represents > 2.0‐fold expansion of HPC and TNC, respectively compared to cytokine control. Expansion data of HPC and TNC for lead compound C7 is highlighted with red circles. *, p

    Article Snippet: As described previously, mononuclear cells (MNC) were isolated from the fresh UCB by density gradient centrifugation using Ficoll‐Histopaque Premium (GE Healthcare, U.K.) .

    Techniques:

    Grafts generated by expanding UCB–MNC in presence of C7 resulted in enhanced engraftment of human cells in the PB and bone marrow (BM) of NSG mice week 2–3 of post‐transplantation. (A): Human CD45 chimerism in PB of NSG mice at week 3 post‐transplantation with non‐expanded or expanded UCB. Expansion of the UCB grafts was carried out using the mononuclear fraction (i.e., without CD34 selection) in either serum free expansion media or animal component free media that were supplemented with cytokines. The absolute cell dose of non‐expanded graft was 2.5 × 10 7 cells/kg while the expanded grafts (either fresh or frozen‐thawed) were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg. The scatter plot represents the human CD45 chimerism of individual animals and depicts the geometric mean with 95% confidence interval (CI) of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (B): Human CD45 chimerism in PB of male NSG mice transplanted with cytokine control or C7 and cytokine expanded UCB at equivalent cell dosage of 2.5 × 10 7 cells/kg at week 6, 10, and 16 post‐transplantation. Each data point represents the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (C): Lineage commitment of the human CD45 cells those are present in the PB of NSG mice at week 3 post‐transplantation. The absolute cell dose of non‐expanded graft was 5.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 5.0 × 10 7 cells/kg. The scatter plot represents the proportion of monocytes (CD45 + CD33 + ), granulocytes (CD45 + CD15 + ), T cells (CD45 + CD3 + ), and B cells (CD45 + CD19 + ) present among the total human cells in each individual animals and depicts the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (D): Determination of severe combined immunodeficiency repopulating capacity (SRC) frequency by limiting dilution assay using L‐Calc software and Extreme Limiting Dilution Assay. A NSG mouse is considered to be positive if human CD45 chimerism is > 0.40% in PB at week 3. The data is calculated at two transplantation dosage of 2.5 × 10 7 and 5.0 × 10 7 cells/kg for both male and female recipients. (E): A scatter plot of human CD45 chimerism in PB of NSG mice at week 2 post‐transplantation. The absolute cell dose of non‐expanded graft was 10.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 10.0 × 10 7 cells/kg. The scatter plot represents the human CD45 chimerism of individual animals and depicts the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (F): A scatter plot of human CD45 + CD3 + T cell chimerism in PB of NSG mice at week 2 post‐transplantation. The absolute cell dose of non‐expanded graft was 10.0 × 10 7 or 5.0 × 10 7 cells/kg while the C7 expanded grafts were transplanted at equivalent cell dosage of 10.0 × 10 7 or 5.0 × 10 7 cells/kg. The scatter plot represents the human T cell chimerism of individual animals and depicts the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (G): Kaplan‐Meier survival curve of the NSG mice transplanted with C7 or cytokine expanded UCB–MNC and non‐expanded graft over 60‐days observation period. The absolute cell dose of non‐expanded graft was 10.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 10.0 × 10 7 cells/kg. The overall statistical comparisons for the experimental groups are also shown. (H): A scatter plot of human CD45 + cells, CD45 + CD34 + progenitors, CD45 + CD3 + T cells, CD45 + CD19 + B cells and CD45 + CD34 + CD33 + myeloid progenitor cells chimerism in BM of female NSG mice at week 2 post‐transplantation. The absolute cell dose of non‐expanded graft was 10.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 10.0 × 10 7 cells/kg. The scatter plot represents the human CD45 + cells, CD45 + CD34 + progenitors, CD45 + CD3 + T cells, CD45 + CD19 + B cells, and CD45 + CD34 + CD33 + myeloid progenitor cells chimerism of individual animals and depicts the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. Abbreviations: MNC, mononucleated cells; PB, peripheral blood; UCB, umbilical cord blood.

    Journal: Stem Cells Translational Medicine

    Article Title: Ex Vivo Expansion of CD34+CD90+CD49f+ Hematopoietic Stem and Progenitor Cells from Non‐Enriched Umbilical Cord Blood with Azole Compounds

    doi: 10.1002/sctm.17-0251

    Figure Lengend Snippet: Grafts generated by expanding UCB–MNC in presence of C7 resulted in enhanced engraftment of human cells in the PB and bone marrow (BM) of NSG mice week 2–3 of post‐transplantation. (A): Human CD45 chimerism in PB of NSG mice at week 3 post‐transplantation with non‐expanded or expanded UCB. Expansion of the UCB grafts was carried out using the mononuclear fraction (i.e., without CD34 selection) in either serum free expansion media or animal component free media that were supplemented with cytokines. The absolute cell dose of non‐expanded graft was 2.5 × 10 7 cells/kg while the expanded grafts (either fresh or frozen‐thawed) were transplanted at equivalent cell dosage of 2.5 × 10 7 cells/kg. The scatter plot represents the human CD45 chimerism of individual animals and depicts the geometric mean with 95% confidence interval (CI) of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (B): Human CD45 chimerism in PB of male NSG mice transplanted with cytokine control or C7 and cytokine expanded UCB at equivalent cell dosage of 2.5 × 10 7 cells/kg at week 6, 10, and 16 post‐transplantation. Each data point represents the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (C): Lineage commitment of the human CD45 cells those are present in the PB of NSG mice at week 3 post‐transplantation. The absolute cell dose of non‐expanded graft was 5.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 5.0 × 10 7 cells/kg. The scatter plot represents the proportion of monocytes (CD45 + CD33 + ), granulocytes (CD45 + CD15 + ), T cells (CD45 + CD3 + ), and B cells (CD45 + CD19 + ) present among the total human cells in each individual animals and depicts the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (D): Determination of severe combined immunodeficiency repopulating capacity (SRC) frequency by limiting dilution assay using L‐Calc software and Extreme Limiting Dilution Assay. A NSG mouse is considered to be positive if human CD45 chimerism is > 0.40% in PB at week 3. The data is calculated at two transplantation dosage of 2.5 × 10 7 and 5.0 × 10 7 cells/kg for both male and female recipients. (E): A scatter plot of human CD45 chimerism in PB of NSG mice at week 2 post‐transplantation. The absolute cell dose of non‐expanded graft was 10.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 10.0 × 10 7 cells/kg. The scatter plot represents the human CD45 chimerism of individual animals and depicts the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (F): A scatter plot of human CD45 + CD3 + T cell chimerism in PB of NSG mice at week 2 post‐transplantation. The absolute cell dose of non‐expanded graft was 10.0 × 10 7 or 5.0 × 10 7 cells/kg while the C7 expanded grafts were transplanted at equivalent cell dosage of 10.0 × 10 7 or 5.0 × 10 7 cells/kg. The scatter plot represents the human T cell chimerism of individual animals and depicts the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. (G): Kaplan‐Meier survival curve of the NSG mice transplanted with C7 or cytokine expanded UCB–MNC and non‐expanded graft over 60‐days observation period. The absolute cell dose of non‐expanded graft was 10.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 10.0 × 10 7 cells/kg. The overall statistical comparisons for the experimental groups are also shown. (H): A scatter plot of human CD45 + cells, CD45 + CD34 + progenitors, CD45 + CD3 + T cells, CD45 + CD19 + B cells and CD45 + CD34 + CD33 + myeloid progenitor cells chimerism in BM of female NSG mice at week 2 post‐transplantation. The absolute cell dose of non‐expanded graft was 10.0 × 10 7 cells/kg while the expanded grafts were transplanted at equivalent cell dosage of 10.0 × 10 7 cells/kg. The scatter plot represents the human CD45 + cells, CD45 + CD34 + progenitors, CD45 + CD3 + T cells, CD45 + CD19 + B cells, and CD45 + CD34 + CD33 + myeloid progenitor cells chimerism of individual animals and depicts the geometric mean with 95% CI of respective treatments. p values generated from Mann‐Whitney U test among indicated experimental groups are shown in the graph for the stated n values. Abbreviations: MNC, mononucleated cells; PB, peripheral blood; UCB, umbilical cord blood.

    Article Snippet: As described previously, mononuclear cells (MNC) were isolated from the fresh UCB by density gradient centrifugation using Ficoll‐Histopaque Premium (GE Healthcare, U.K.) .

    Techniques: Generated, Mouse Assay, Transplantation Assay, Selection, MANN-WHITNEY, Limiting Dilution Assay, Software

    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Centrifugation, Enzyme-linked Immunosorbent Assay

    Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Expressing, Centrifugation, Staining, Flow Cytometry, Cytometry

    Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Centrifugation, Staining, Flow Cytometry, Cytometry