monodansylcadaverine mdc Millipore Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Millipore monodansylcadaverine mdc
    <t>Monodansylcadaverine</t> <t>(MDC)</t> stains autophagic K562 cells with sensitivity equivalent to LC3B immunoblotting and GFP-LC3B fluorescence microscopy. ( A ) Fluorescence microscopy. K562 cells were treated with dimethyl sulfoxide (DMSO) or 1 μM imatinib (IM) overnight followed by MDC staining. Images of live cells were taken at different exposure times (milliseconds, ms) using an inverted fluorescence microscope. Scale bar: 25 μm. ( B ) MDC Fluorescence spectrophotometry. K562 cells treated with DMSO or IM were stained with MDC. MDC fluorescence of live K562 cells was quantified using a micro-plate reader at an excitation wavelength of 335 nm and an emission wavelength of 525 nm. The relative MDC levels were obtained by dividing MDC fluorescence of IM-treated cells to that of DMSO-treated cells. Error bars represent three independent experiments. ( C ) LC3B immunoblotting. Cropped images are shown and full images are included in supplemental materials. ACTB (β actin) is the loading control. The intensities of LC3B-II or ACTB were quantified using Image J. The fold changes of LC3B-II/ACTB were obtained by dividing the ratio of LC3B-II/ACTB in IM-treated cells with that of DMSO-treated cells. ( D ) MDC staining of K562 cells treated with IM and/or bafilomycin A1 (BFA1). K562 cells were treated with a combination of 1 μM IM (10 hours) and 5 nM of BFA1 (48 hours). Cells were imaged using a fluorescence confocal microscope. ( E ) Quantification of MDC intensities. Fluorescence intensities of MDC in 10 different cells from three images were quantified using Image J. ( F ) GFP-LC3B fluorescence microscopy. K562 cells were stably transfected with a construct encoding GFP-LC3B. Cells were treated with a combination of IM and BFA1 and then imaged. ( G ) Quantification of cells with GFP-LC3B puncta. Four to five images of more than thirty K562 cells from each treatment were randomly selected for the following quantification analyses. GFP-expressing K562 cells or K562 cells with GFP puncta (indicating autophagy) were counted by three persons. Percentages of K562 cells with puncta were obtained by dividing numbers of K562 cells with GFP puncta with those of GFP-expressing cells. Scale bar: 25 nm.
    Monodansylcadaverine Mdc, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monodansylcadaverine mdc/product/Millipore
    Average 95 stars, based on 492 article reviews
    Price from $9.99 to $1999.99
    monodansylcadaverine mdc - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    77
    Millipore tg2 substrate monodansylcadaverine mdc
    Endothelial extracellular transglutaminase 2 <t>(TG2)</t> activity analyses by <t>monodansylcadaverine</t> <t>(MDC)</t> incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value
    Tg2 Substrate Monodansylcadaverine Mdc, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tg2 substrate monodansylcadaverine mdc/product/Millipore
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tg2 substrate monodansylcadaverine mdc - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    78
    Millipore autophagy assay monodansylcadaverine
    Endothelial extracellular transglutaminase 2 <t>(TG2)</t> activity analyses by <t>monodansylcadaverine</t> <t>(MDC)</t> incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value
    Autophagy Assay Monodansylcadaverine, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/autophagy assay monodansylcadaverine/product/Millipore
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    autophagy assay monodansylcadaverine - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    75
    Millipore lysotracker red staining monodansylcadaverine
    Endothelial extracellular transglutaminase 2 <t>(TG2)</t> activity analyses by <t>monodansylcadaverine</t> <t>(MDC)</t> incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value
    Lysotracker Red Staining Monodansylcadaverine, supplied by Millipore, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysotracker red staining monodansylcadaverine/product/Millipore
    Average 75 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lysotracker red staining monodansylcadaverine - by Bioz Stars, 2020-02
    75/100 stars
      Buy from Supplier

    91
    Millipore monodansylcadaverin mdc
    Endothelial extracellular transglutaminase 2 <t>(TG2)</t> activity analyses by <t>monodansylcadaverine</t> <t>(MDC)</t> incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value
    Monodansylcadaverin Mdc, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monodansylcadaverin mdc/product/Millipore
    Average 91 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    monodansylcadaverin mdc - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    80
    Millipore monodansylcadverine mdc
    Endothelial extracellular transglutaminase 2 <t>(TG2)</t> activity analyses by <t>monodansylcadaverine</t> <t>(MDC)</t> incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value
    Monodansylcadverine Mdc, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monodansylcadverine mdc/product/Millipore
    Average 80 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    monodansylcadverine mdc - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    95
    Millipore 3 methyladenine 3 ma monodansylcadaverine
    Endothelial extracellular transglutaminase 2 <t>(TG2)</t> activity analyses by <t>monodansylcadaverine</t> <t>(MDC)</t> incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value
    3 Methyladenine 3 Ma Monodansylcadaverine, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 methyladenine 3 ma monodansylcadaverine/product/Millipore
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    3 methyladenine 3 ma monodansylcadaverine - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    81
    Millipore monodansylcadaverin
    Endothelial extracellular transglutaminase 2 <t>(TG2)</t> activity analyses by <t>monodansylcadaverine</t> <t>(MDC)</t> incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value
    Monodansylcadaverin, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monodansylcadaverin/product/Millipore
    Average 81 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    monodansylcadaverin - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    80
    Millipore monodancylcadaverin mdc
    Endothelial extracellular transglutaminase 2 <t>(TG2)</t> activity analyses by <t>monodansylcadaverine</t> <t>(MDC)</t> incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value
    Monodancylcadaverin Mdc, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monodancylcadaverin mdc/product/Millipore
    Average 80 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    monodancylcadaverin mdc - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    80
    Millipore monodansycadaverine mdc staining mdc
    Endothelial extracellular transglutaminase 2 <t>(TG2)</t> activity analyses by <t>monodansylcadaverine</t> <t>(MDC)</t> incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value
    Monodansycadaverine Mdc Staining Mdc, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monodansycadaverine mdc staining mdc/product/Millipore
    Average 80 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    monodansycadaverine mdc staining mdc - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    Image Search Results


    Monodansylcadaverine (MDC) stains autophagic K562 cells with sensitivity equivalent to LC3B immunoblotting and GFP-LC3B fluorescence microscopy. ( A ) Fluorescence microscopy. K562 cells were treated with dimethyl sulfoxide (DMSO) or 1 μM imatinib (IM) overnight followed by MDC staining. Images of live cells were taken at different exposure times (milliseconds, ms) using an inverted fluorescence microscope. Scale bar: 25 μm. ( B ) MDC Fluorescence spectrophotometry. K562 cells treated with DMSO or IM were stained with MDC. MDC fluorescence of live K562 cells was quantified using a micro-plate reader at an excitation wavelength of 335 nm and an emission wavelength of 525 nm. The relative MDC levels were obtained by dividing MDC fluorescence of IM-treated cells to that of DMSO-treated cells. Error bars represent three independent experiments. ( C ) LC3B immunoblotting. Cropped images are shown and full images are included in supplemental materials. ACTB (β actin) is the loading control. The intensities of LC3B-II or ACTB were quantified using Image J. The fold changes of LC3B-II/ACTB were obtained by dividing the ratio of LC3B-II/ACTB in IM-treated cells with that of DMSO-treated cells. ( D ) MDC staining of K562 cells treated with IM and/or bafilomycin A1 (BFA1). K562 cells were treated with a combination of 1 μM IM (10 hours) and 5 nM of BFA1 (48 hours). Cells were imaged using a fluorescence confocal microscope. ( E ) Quantification of MDC intensities. Fluorescence intensities of MDC in 10 different cells from three images were quantified using Image J. ( F ) GFP-LC3B fluorescence microscopy. K562 cells were stably transfected with a construct encoding GFP-LC3B. Cells were treated with a combination of IM and BFA1 and then imaged. ( G ) Quantification of cells with GFP-LC3B puncta. Four to five images of more than thirty K562 cells from each treatment were randomly selected for the following quantification analyses. GFP-expressing K562 cells or K562 cells with GFP puncta (indicating autophagy) were counted by three persons. Percentages of K562 cells with puncta were obtained by dividing numbers of K562 cells with GFP puncta with those of GFP-expressing cells. Scale bar: 25 nm.

    Journal: Scientific Reports

    Article Title: A large-scale RNA interference screen identifies genes that regulate autophagy at different stages

    doi: 10.1038/s41598-018-21106-5

    Figure Lengend Snippet: Monodansylcadaverine (MDC) stains autophagic K562 cells with sensitivity equivalent to LC3B immunoblotting and GFP-LC3B fluorescence microscopy. ( A ) Fluorescence microscopy. K562 cells were treated with dimethyl sulfoxide (DMSO) or 1 μM imatinib (IM) overnight followed by MDC staining. Images of live cells were taken at different exposure times (milliseconds, ms) using an inverted fluorescence microscope. Scale bar: 25 μm. ( B ) MDC Fluorescence spectrophotometry. K562 cells treated with DMSO or IM were stained with MDC. MDC fluorescence of live K562 cells was quantified using a micro-plate reader at an excitation wavelength of 335 nm and an emission wavelength of 525 nm. The relative MDC levels were obtained by dividing MDC fluorescence of IM-treated cells to that of DMSO-treated cells. Error bars represent three independent experiments. ( C ) LC3B immunoblotting. Cropped images are shown and full images are included in supplemental materials. ACTB (β actin) is the loading control. The intensities of LC3B-II or ACTB were quantified using Image J. The fold changes of LC3B-II/ACTB were obtained by dividing the ratio of LC3B-II/ACTB in IM-treated cells with that of DMSO-treated cells. ( D ) MDC staining of K562 cells treated with IM and/or bafilomycin A1 (BFA1). K562 cells were treated with a combination of 1 μM IM (10 hours) and 5 nM of BFA1 (48 hours). Cells were imaged using a fluorescence confocal microscope. ( E ) Quantification of MDC intensities. Fluorescence intensities of MDC in 10 different cells from three images were quantified using Image J. ( F ) GFP-LC3B fluorescence microscopy. K562 cells were stably transfected with a construct encoding GFP-LC3B. Cells were treated with a combination of IM and BFA1 and then imaged. ( G ) Quantification of cells with GFP-LC3B puncta. Four to five images of more than thirty K562 cells from each treatment were randomly selected for the following quantification analyses. GFP-expressing K562 cells or K562 cells with GFP puncta (indicating autophagy) were counted by three persons. Percentages of K562 cells with puncta were obtained by dividing numbers of K562 cells with GFP puncta with those of GFP-expressing cells. Scale bar: 25 nm.

    Article Snippet: PP242, chloroquine, bafilomycin A1 and monodansylcadaverine (MDC) were purchased from Sigma-Aldrich.

    Techniques: Fluorescence, Microscopy, Staining, Mass Spectrometry, Spectrophotometry, Stable Transfection, Transfection, Construct, Expressing

    Endothelial extracellular transglutaminase 2 (TG2) activity analyses by monodansylcadaverine (MDC) incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value

    Journal: PLoS ONE

    Article Title: Thioredoxin Is Involved in Endothelial Cell Extracellular Transglutaminase 2 Activation Mediated by Celiac Disease Patient IgA

    doi: 10.1371/journal.pone.0077277

    Figure Lengend Snippet: Endothelial extracellular transglutaminase 2 (TG2) activity analyses by monodansylcadaverine (MDC) incorporation. The relative extracellular TG2 activity ( A ) at different time points and with ( B ) increasing concentrations of class A immunoglobulins are shown. The dashed line indicates TG2 activity in untreated endothelial cells (HUVECs). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value

    Article Snippet: At indicated time-points the cells were washed with Hanks Balanced Salt Solution (HBSS; Sigma Aldrich, St. Louis, MI), followed by incubation with the TG2 substrate monodansylcadaverine (MDC) (0.2 mM; Sigma Aldrich) for 2h at 37°C.

    Techniques: Activity Assay

    Endothelial extracellular matrix (ECM) transglutaminase 2 (TG2) activity analyses by monodansylcadaverine (MDC) incorporation. ( A ) The relative ECM-TG2 activity at different time points by ELISA. The dashed line indicates TG2 activity in untreated endothelial cells (HUVEC). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value

    Journal: PLoS ONE

    Article Title: Thioredoxin Is Involved in Endothelial Cell Extracellular Transglutaminase 2 Activation Mediated by Celiac Disease Patient IgA

    doi: 10.1371/journal.pone.0077277

    Figure Lengend Snippet: Endothelial extracellular matrix (ECM) transglutaminase 2 (TG2) activity analyses by monodansylcadaverine (MDC) incorporation. ( A ) The relative ECM-TG2 activity at different time points by ELISA. The dashed line indicates TG2 activity in untreated endothelial cells (HUVEC). Bars represent mean MDC incorporation as fold of control and error bars indicate standard error of the mean. P-value

    Article Snippet: At indicated time-points the cells were washed with Hanks Balanced Salt Solution (HBSS; Sigma Aldrich, St. Louis, MI), followed by incubation with the TG2 substrate monodansylcadaverine (MDC) (0.2 mM; Sigma Aldrich) for 2h at 37°C.

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay