Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
Article Title: Preconception Exposure to Fine Particulate Matter Leads to Cardiac Dysfunction in Adult Male Offspring
Figure Lengend Snippet: Quantitative polymerase chain reaction expression of ( A ) IL ‐6, ( B ) IL ‐15, ( C ) TNF ‐α, ( D ) NF ‐κB, ( E ) CRP , ( F ) E‐selectin ( CD 26E), ( G ) P‐selectin ( CD 26P), ( H ) ICAM ‐1. I, Western blot analysis showing expression of MCP ‐1. Representative Western blot was shown on the right and quantification on the left. Data were obtained from 3‐month‐old mouse hearts (n=6 mice/group) that were born to preconception FA or PM 2.5 exposed parents and expressed as mean±SEM. CRP indicates C‐reactive protein; ICAM1, intercellular adhesion molecule 1; IL‐6, interleukin 6; IL‐15, interleukin 15; MCP‐1, monocyte chemoattractant protein‐1; NF ‐κB, nuclear factor kappa‐light‐chain‐enhancer of activated B cells; PC FA, preconception filtered air; PC PM 2.5 , preconception particulate matter (
Article Snippet: Membranes were probed with antibodies to sarco/endoplasmic reticulum Ca2+ ‐ATPase (SERCA2a) (Thermo Fisher Scientific, MA3‐919, Waltham, MA), phosphorylated phospholamban (p‐PLN) (Santa Cruz Biotechnology, sc‐12963, Dallas, TX), Na+ /Ca2+ exchanger (NCX) (Thermo Fisher Scientific, MA3‐926, Waltham, MA), Nitric oxide synthase 2 (NOS2) (Santa Cruz Biotechnology, sc‐651, Dallas, TX), DNA methyltransferase 1 (DNMT1) (Abcam, ab‐13537, Cambridge, MA), DNA methyltransferase 3A (DNMT3A) (Abcam, ab‐13888, Cambridge, MA), monocyte chemoattractant protein‐1 (MCP‐1) (Santa Cruz Biotechnology, sc‐136750, Dallas, TX), and collagen type III alpha 1 chain (Santa Cruz Biotechnology, sc‐8780‐R, Dallas, TX).
Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Mouse Assay