monocyte chemoattractant protein-1 Search Results


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  • 96
    Millipore monocyte chemotactic protein 1
    Mucosal–plasma chemokine gradients in women who remained uninfected (controls; n = 50) and in HIV- women who subsequently acquired HIV (cases; n = 57) during the CAPRISA 004 trial. Intercompartmental cytokine gradients were determined by quantifying the difference between standardized cytokine concentrations of the female genital tract and blood plasma for each participant. Four of the 5 gradients associated with HIV outcome in multivariate analysis are depicted here: (A) IP-10, (B) MIP-1β, (C) IL-8, and (D) monocyte chemotactic <t>protein-1</t> (MCP-1). “Positive” gradients are indicative of higher relative levels in the genital tract compared with the blood.
    Monocyte Chemotactic Protein 1, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson monocyte chemoattractant protein 1
    Plasminogen activator inhibitor‐1 ( PAI ‐1) regulates cytokine production during pneumonic plague. Forty‐eight hours after inoculation with phosphate‐buffered saline (mock), 10 4 colony‐forming units ( CFU s) of Yersinia pestis , or 10 4 CFU s of Y. pestis ∆ pla , the levels of the indicated cytokines present in the bronchoalveolar lavage fluid of C57 BL /6 or C57 BL /6 PAI ‐1 −/− mice were assessed by cytometric bead array. Data from two or three independent experiments are combined ( n = 10–15 for each group); error bars represent standard errors of the mean. One‐way anova with Bonferroni's multiple comparison test was used to determine significance (* P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001). IFN , interferon; IL , interleukin; MCP ‐1, monocyte <t>chemoattractant</t> <t>protein‐1;</t> NS, not significant; TNF , tumor necrosis factor.
    Monocyte Chemoattractant Protein 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 625 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad monocyte chemoattractant protein 1
    Association between overall radiology and inflammatory marker concentrations. These box and whisker plots demonstrate the median, interquartile range, and range of the highest inflammatory marker concentrations in lumbar and ventricular cerebrospinal fluid (CSF) for various radiologic features recorded across admission and in-hospital follow-up scans (overall computed tomography and magnetic resonance imaging). Statistically significant results are indicated by a square bracket and *. Each row represents the data for individual cytokines (interleukin [IL] 12p40, interferon-inducible protein 10 [IP-10], monocyte <t>chemoattractant</t> <t>protein</t> 1 [MCP-1], tumor necrosis factor alpha [TNF-α], macrophage inflammatory protein 1-alpha [MIP-1α], IL-6, and IL-8) in the presence of parenchymal tuberculomas (top panel) or infarcts (bottom panel).
    Monocyte Chemoattractant Protein 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology monocyte chemoattractant protein 1
    Pioglitazone (Pio) protects pancreatic β-cells and regulates blood glucose levels in high-fat (HF)-diet-induced diabetic mice. The pancreatic islet from KK-Ay mice, which were fed an HF diet with or without Pio, were examined by double-immunofluorescence for (A) insulin-activating transcription factor 6 and insulin-glucose-regulated protein 78 (GRP78), (B) insulin-monocyt e <t>chemoattractant</t> <t>protein-1</t> and insulin-glucagon (GLU), (C) insulin-glucose transporter 2 (GLUT2). (A-C) The nucleus was visualized by 4′,6-diamidino-2-phenylindole (DAPI). Bars=20 µm. (D) Blood glucose levels and body weight were measured every week. INS, insulin; ATF6, activating transcription factor 6; MCP1, monocyte chemoattractant protein-1. a P
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    Abcam monocyte chemoattractant protein 1
    Arginase inhibition or EC-A1 deletion prevents HFHS-induced increased in expression of cytokines, chemokines and adhesion molecules in isolated aortic endothelial cells. Effects of ABH and EC-A1 deletion on protein levels of the proinflammatory cytokine tumor necrosis factor-α (TNF-α; A ), the chemokine monocyte <t>chemoattractant</t> <t>protein-1</t> (MCP-1; B ), anti-inflammatory interleutin-10 (IL-10; C ), adhesion molecules vascular cell adhesion molecule-1 (VCAM-1; D ), and intercellular adhesion molecule 1 (ICAM; E ). Values are means ± SE; n = 6 mice. * P
    Monocyte Chemoattractant Protein 1, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson monocyte chemoattractant protein mcp 1
    Release of cytokines by purified natural killer (NK) cells upon stimulation with selected expansion protocols. Purified NK cells were cultured using different stimulation protocols. On day 6, release of tumor necrosis factor (TNF)-α, IFN-γ, macrophage inflammatory protein (MIP)-1α, monocyte <t>chemoattractant</t> protein <t>(MCP)-1,</t> granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-8, and IL-10 were assayed by cytometric bead array analyses. Graphs show mean values and SDs obtained from four independent donors, except GM-CSF, which was measured for three independent donors. Asterisks indicate significant differences (* p
    Monocyte Chemoattractant Protein Mcp 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher monocyte chemoattractant protein 1
    Mast cells do not influence the production of interleukin-6 (IL-6), monocyte <t>chemoattractant</t> protein 1 (MCP-1), IL-3 and IL-13 in the peritoneum after Staphylococcus aureus infection. Mcpt5-Cre + × R-DTA and Mcpt5-Cre − × R-DTA were infected intraperitoneally with S. aureus . As a control, TSB medium only (bacterial growth medium) was injected. After 4 hr or 1 day, peritoneal lavage was performed. Peritoneal lavage fluids were analysed for levels of IL-6 (a), MCP-1 (b), IL-3 (c) and IL-13 (d) by ELISA. Mean ± SEM ( n = 5 to n = 11). * P
    Monocyte Chemoattractant Protein 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 419 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pharmingen monocyte chemoattractant protein 1
    Mast cells do not influence the production of interleukin-6 (IL-6), monocyte <t>chemoattractant</t> protein 1 (MCP-1), IL-3 and IL-13 in the peritoneum after Staphylococcus aureus infection. Mcpt5-Cre + × R-DTA and Mcpt5-Cre − × R-DTA were infected intraperitoneally with S. aureus . As a control, TSB medium only (bacterial growth medium) was injected. After 4 hr or 1 day, peritoneal lavage was performed. Peritoneal lavage fluids were analysed for levels of IL-6 (a), MCP-1 (b), IL-3 (c) and IL-13 (d) by ELISA. Mean ± SEM ( n = 5 to n = 11). * P
    Monocyte Chemoattractant Protein 1, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam monocyte chemoattractant protein mcp 1
    Immunofluorescence staining of <t>MCP-1</t> and CCL22 in an area with a high degree of VSMC density. Fig. depict the same area as Fig. 1B- β . Immunofluorescence staining showed monocyte <t>chemoattractant</t> protein (MCP)-1 (clone 2.2-4H5-01A11, 10 µg/ml PeproTech Inc., Rocky Hill, NJ; red) positive cells were negative for CCL22 (green). Bars indicate 50 µm.
    Monocyte Chemoattractant Protein Mcp 1, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam monocyte chemoattractant protein 1 mcp 1
    Monocyte <t>chemoattractant</t> <t>protein</t> 1 (MCP1) and haptoglobin (Hp) are affected in their capacity to migrate by CCR2 antagonist (RS1028959 exposition). Wound-healing assays were performed on SKOV-3 cells for 0, 12, 24 and 48 h at the indicated doses of Hp (250 μg) and monocyte chemoattractant protein 1 (MCP1, 250 ng). In addition, the effect of pretreatment with the chemokine (C-C motif) receptor 2 (CCR2) synthetic antagonist RS102895 (5 μM) on the capacity of SKOV-3 cells to migrate toward MCP1 (250 ng/ml) and Hp (0.250 mg/ml) is shown. As a negative control, bovine serum albumin (BSA) (1 mg/ml) was used. Chemotaxis was measured by confocal microscopy; nuclei (blue) were stained with DAPI (1:50). ( B ) Quantitative analysis of migration by the software Zen 2011 (Blue edition, Carl Zeiss) considering an average area of 9 × 10 5 μm 2 . This program determines the mean fluorescence intensity of each condition, which is plotted as a histogram. The results correspond to the average of 3 independent experiments. Bar scale = 100 μm.
    Monocyte Chemoattractant Protein 1 Mcp 1, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher monocyte chemoattractant protein 1 mcp 1
    Circulating inflammatory markers. Animals were fed standard chow (ST) or cafeteria diet (CAF) and trained 5 days per week for 30 min on a treadmill at different intensities (CON: 0; TML: 12; TMH: 17 m/min). Diets and training sessions were continued for 8 weeks. The serum levels of the cytokines monocyte <t>chemoattractant</t> <t>protein-1</t> <t>(MCP-1)</t> and C-reactive protein (CRP) were determined at the end of the experiment. The data are given as the mean ± SEM (n = 9–12). D : the effect of diet; E : the effect of exercise (p
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    Abcam anti monocyte chemoattractant protein 1
    SsnB administration modulates Kupffer cell activation in early steatohepatitic injury. A : qRT-PCR analysis of mRNA expression of CD68 and monocyte <t>chemoattractant</t> <t>protein-1</t> (MCP1) from liver homogenates of S, SH, and SH+SsnB mice, normalized against S
    Anti Monocyte Chemoattractant Protein 1, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson monocyte chemoattractant protein 1 mcp 1
    Anti-inflammatory effect of A. scholaris extract (ASE) on HaCaT human keratinocytes treated with all-trans retinoic acid (ATRA), as assessed by monitoring the protein expression of (a) monocyte <t>chemoattractant</t> <t>protein-1</t> <t>(MCP-1)</t> and (b) interleukin-8 (IL-8). All results are expressed as average ± SD. * P
    Monocyte Chemoattractant Protein 1 Mcp 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems monocyte chemoattractant protein 1
    Anti-inflammatory effect of A. scholaris extract (ASE) on HaCaT human keratinocytes treated with all-trans retinoic acid (ATRA), as assessed by monitoring the protein expression of (a) monocyte <t>chemoattractant</t> <t>protein-1</t> <t>(MCP-1)</t> and (b) interleukin-8 (IL-8). All results are expressed as average ± SD. * P
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    Bio-Rad monocyte chemoattractant protein mcp 1
    Receiver operating characteristic curves of Acute Physiology and Chronic Health (APACHE) II score, monocyte <t>chemoattractant</t> protein <t>(MCP)-1</t> and a composite variable (APACHE II + MCP-1) predicting mortality. Shown are areas under receiver operating characteristic curves (AUROCs) for APACHE II score, monocyte MCP-1 and a composite variable (APACHE II + MCP-1), created according to the final model from multivariable analysis (Table 4).
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    Cell Signaling Technology Inc monocyte chemoattractant protein 1 mcp 1
    Serial changes of <t>MCP-1</t> expressions under IHC microscopic findings in kidney specimens. A. Illustrating the time intervals (i.e., 0, 6, 24 and 168 h) of cross section of IR kidney. B. Illustrating the immunohistochemical (IHC) stain (100x) for identification of monocyte <t>chemoattractant</t> protein (MCP)-1 expressions (gray color) in three different anatomical layers, including (1) inner stripe of outer medulla (ISOM), (2) outer stripe of outer medulla (OSOM) and (3) cortex in different time intervals (i.e., 0, 6, 24, 72 and 168 h) after acute kidney IR procedure. C. Analytical results of MCP-1 positively stained area in rat after acute kidney IR procedure: 1) ISOM layer, baseline vs. different time points (i.e., A, B, C), p
    Monocyte Chemoattractant Protein 1 Mcp 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 89/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meso Scale Diagnostics LLC monocyte chemotactic protein 1
    Serial changes of <t>MCP-1</t> expressions under IHC microscopic findings in kidney specimens. A. Illustrating the time intervals (i.e., 0, 6, 24 and 168 h) of cross section of IR kidney. B. Illustrating the immunohistochemical (IHC) stain (100x) for identification of monocyte <t>chemoattractant</t> protein (MCP)-1 expressions (gray color) in three different anatomical layers, including (1) inner stripe of outer medulla (ISOM), (2) outer stripe of outer medulla (OSOM) and (3) cortex in different time intervals (i.e., 0, 6, 24, 72 and 168 h) after acute kidney IR procedure. C. Analytical results of MCP-1 positively stained area in rat after acute kidney IR procedure: 1) ISOM layer, baseline vs. different time points (i.e., A, B, C), p
    Monocyte Chemotactic Protein 1, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti mcp1 antibody
    The expression of inflammatory cytokines and fibrotic factors are reduced in STZ-induced diabetic rats. ( A ) The kidney tissues were fixed in formalin and then subjected to immunofluorescence detection of TNF-α (arrow heads pointing to dark-brown dots indicating TNF-α expression). n = 5 per group, ( B ) IL6 protein level ( C ) <t>MCP1</t> protein level ( D ) TGF-β mRNA level, ( E ) TGF-β protein level with a representative blot, ( F ) Fibronectin (FN) mRNA level in kidney tissues. Data are shown as the means ± SEM *p
    Anti Mcp1 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore silu lite ccl2 c c motif chemokine 2 human
    The expression of inflammatory cytokines and fibrotic factors are reduced in STZ-induced diabetic rats. ( A ) The kidney tissues were fixed in formalin and then subjected to immunofluorescence detection of TNF-α (arrow heads pointing to dark-brown dots indicating TNF-α expression). n = 5 per group, ( B ) IL6 protein level ( C ) <t>MCP1</t> protein level ( D ) TGF-β mRNA level, ( E ) TGF-β protein level with a representative blot, ( F ) Fibronectin (FN) mRNA level in kidney tissues. Data are shown as the means ± SEM *p
    Silu Lite Ccl2 C C Motif Chemokine 2 Human, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti monocyte chemoattractant protein 1 mcp 1
    The expression of inflammatory cytokines and fibrotic factors are reduced in STZ-induced diabetic rats. ( A ) The kidney tissues were fixed in formalin and then subjected to immunofluorescence detection of TNF-α (arrow heads pointing to dark-brown dots indicating TNF-α expression). n = 5 per group, ( B ) IL6 protein level ( C ) <t>MCP1</t> protein level ( D ) TGF-β mRNA level, ( E ) TGF-β protein level with a representative blot, ( F ) Fibronectin (FN) mRNA level in kidney tissues. Data are shown as the means ± SEM *p
    Anti Monocyte Chemoattractant Protein 1 Mcp 1, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Trinity Biotech rabbit anti mouse monocyte chemoattractant protein 1
    The expression of inflammatory cytokines and fibrotic factors are reduced in STZ-induced diabetic rats. ( A ) The kidney tissues were fixed in formalin and then subjected to immunofluorescence detection of TNF-α (arrow heads pointing to dark-brown dots indicating TNF-α expression). n = 5 per group, ( B ) IL6 protein level ( C ) <t>MCP1</t> protein level ( D ) TGF-β mRNA level, ( E ) TGF-β protein level with a representative blot, ( F ) Fibronectin (FN) mRNA level in kidney tissues. Data are shown as the means ± SEM *p
    Rabbit Anti Mouse Monocyte Chemoattractant Protein 1, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Mucosal–plasma chemokine gradients in women who remained uninfected (controls; n = 50) and in HIV- women who subsequently acquired HIV (cases; n = 57) during the CAPRISA 004 trial. Intercompartmental cytokine gradients were determined by quantifying the difference between standardized cytokine concentrations of the female genital tract and blood plasma for each participant. Four of the 5 gradients associated with HIV outcome in multivariate analysis are depicted here: (A) IP-10, (B) MIP-1β, (C) IL-8, and (D) monocyte chemotactic protein-1 (MCP-1). “Positive” gradients are indicative of higher relative levels in the genital tract compared with the blood.

    Journal: Journal of Acquired Immune Deficiency Syndromes (1999)

    Article Title: Genital—Systemic Chemokine Gradients and the Risk of HIV Acquisition in Women

    doi: 10.1097/QAI.0000000000001218

    Figure Lengend Snippet: Mucosal–plasma chemokine gradients in women who remained uninfected (controls; n = 50) and in HIV- women who subsequently acquired HIV (cases; n = 57) during the CAPRISA 004 trial. Intercompartmental cytokine gradients were determined by quantifying the difference between standardized cytokine concentrations of the female genital tract and blood plasma for each participant. Four of the 5 gradients associated with HIV outcome in multivariate analysis are depicted here: (A) IP-10, (B) MIP-1β, (C) IL-8, and (D) monocyte chemotactic protein-1 (MCP-1). “Positive” gradients are indicative of higher relative levels in the genital tract compared with the blood.

    Article Snippet: In brief, the concentrations of IL-1α, IL-1β, IL-6, IL-7, IL-8, IL-10, granulocyte-macrophage colony-stimulating factor, IP-10, monocyte chemotactic protein-1, MIP-1α, MIP-1β, and TNF-α were measured preinfection (median of 20 weeks preinfection, range 2–71 weeks) using Milliplex High Sensitivity and Human Cytokine kits according to the manufacturer's protocol (Millipore, Billerica, MA).

    Techniques:

    The scattered plots of serum nerve growth factor, interleukin (IL) -1β, IL-6, IL-8, tumor necrosis factor (TNF) -α, and monocyte chemotactic protein (MCP)-1 among the controls, OAB-dry and OAB-wet patients.

    Journal: PLoS ONE

    Article Title: Increased Serum Adipokines Implicate Chronic Inflammation in the Pathogenesis of Overactive Bladder Syndrome Refractory to Antimuscarinic Therapy

    doi: 10.1371/journal.pone.0076706

    Figure Lengend Snippet: The scattered plots of serum nerve growth factor, interleukin (IL) -1β, IL-6, IL-8, tumor necrosis factor (TNF) -α, and monocyte chemotactic protein (MCP)-1 among the controls, OAB-dry and OAB-wet patients.

    Article Snippet: Concentrations of NGF and serum adipokines including interleukin (IL) IL-1β, IL-6, IL-8, tumor necrosis factor (TNF)- α, monocyte chemotactic protein (MCP)-1, insulin, and leptin were quantified using a bead-based human serum adipokine panel B kit (Millipore, Billerica, MA, USA).

    Techniques:

    Plasminogen activator inhibitor‐1 ( PAI ‐1) regulates cytokine production during pneumonic plague. Forty‐eight hours after inoculation with phosphate‐buffered saline (mock), 10 4 colony‐forming units ( CFU s) of Yersinia pestis , or 10 4 CFU s of Y. pestis ∆ pla , the levels of the indicated cytokines present in the bronchoalveolar lavage fluid of C57 BL /6 or C57 BL /6 PAI ‐1 −/− mice were assessed by cytometric bead array. Data from two or three independent experiments are combined ( n = 10–15 for each group); error bars represent standard errors of the mean. One‐way anova with Bonferroni's multiple comparison test was used to determine significance (* P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001). IFN , interferon; IL , interleukin; MCP ‐1, monocyte chemoattractant protein‐1; NS, not significant; TNF , tumor necrosis factor.

    Journal: Journal of Thrombosis and Haemostasis

    Article Title: Proteolysis of plasminogen activator inhibitor‐1 by Yersinia pestis remodulates the host environment to promote virulence. Proteolysis of plasminogen activator inhibitor‐1 by Yersinia pestis remodulates the host environment to promote virulence

    doi: 10.1111/jth.13408

    Figure Lengend Snippet: Plasminogen activator inhibitor‐1 ( PAI ‐1) regulates cytokine production during pneumonic plague. Forty‐eight hours after inoculation with phosphate‐buffered saline (mock), 10 4 colony‐forming units ( CFU s) of Yersinia pestis , or 10 4 CFU s of Y. pestis ∆ pla , the levels of the indicated cytokines present in the bronchoalveolar lavage fluid of C57 BL /6 or C57 BL /6 PAI ‐1 −/− mice were assessed by cytometric bead array. Data from two or three independent experiments are combined ( n = 10–15 for each group); error bars represent standard errors of the mean. One‐way anova with Bonferroni's multiple comparison test was used to determine significance (* P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001). IFN , interferon; IL , interleukin; MCP ‐1, monocyte chemoattractant protein‐1; NS, not significant; TNF , tumor necrosis factor.

    Article Snippet: Cytokine analysis At 48 h after inoculation with PBS or Y. pestis , the levels of interleukin (IL)‐12p70, tumor necrosis factor (TNF)‐α, interferon (IFN)‐γ, monocyte chemoattractant protein‐1 (MCP‐1) and IL‐6 were quantitatively established from the supernatant of the collected BALF by use of the cytometric bead array technique (BD Cytometric Bead Array Mouse Inflammation Kit; BD Biosciences, San Jose, CA, USA) as specified by the manufacturer.

    Techniques: Proximity Ligation Assay, Mouse Assay

    Association between overall radiology and inflammatory marker concentrations. These box and whisker plots demonstrate the median, interquartile range, and range of the highest inflammatory marker concentrations in lumbar and ventricular cerebrospinal fluid (CSF) for various radiologic features recorded across admission and in-hospital follow-up scans (overall computed tomography and magnetic resonance imaging). Statistically significant results are indicated by a square bracket and *. Each row represents the data for individual cytokines (interleukin [IL] 12p40, interferon-inducible protein 10 [IP-10], monocyte chemoattractant protein 1 [MCP-1], tumor necrosis factor alpha [TNF-α], macrophage inflammatory protein 1-alpha [MIP-1α], IL-6, and IL-8) in the presence of parenchymal tuberculomas (top panel) or infarcts (bottom panel).

    Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

    Article Title: Biomarkers of Cerebral Injury and Inflammation in Pediatric Tuberculous Meningitis

    doi: 10.1093/cid/cix540

    Figure Lengend Snippet: Association between overall radiology and inflammatory marker concentrations. These box and whisker plots demonstrate the median, interquartile range, and range of the highest inflammatory marker concentrations in lumbar and ventricular cerebrospinal fluid (CSF) for various radiologic features recorded across admission and in-hospital follow-up scans (overall computed tomography and magnetic resonance imaging). Statistically significant results are indicated by a square bracket and *. Each row represents the data for individual cytokines (interleukin [IL] 12p40, interferon-inducible protein 10 [IP-10], monocyte chemoattractant protein 1 [MCP-1], tumor necrosis factor alpha [TNF-α], macrophage inflammatory protein 1-alpha [MIP-1α], IL-6, and IL-8) in the presence of parenchymal tuberculomas (top panel) or infarcts (bottom panel).

    Article Snippet: Interleukin (IL) 1β, IL-1 receptor antagonist (RA), IL-6, IL-10, IL-12p40, tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), IL-8, growth-regulated oncogene (GRO), monocyte chemoattractant protein 1 (MCP-1), interferon-inducible protein 10 (IP-10), macrophage inflammatory protein 1α (MIP-1α), vascular endothelial growth factor (VEGF), and regulated upon activation, normal T-cell–expressed and secreted protein (RANTES) were analyzed on the Bio-Plex platform (Bio-Rad Laboratories, Hercules, California), using customized Milliplex kits (Millipore, St Charles, Missouri), according to manufacturer instructions.

    Techniques: Marker, Whisker Assay, Computed Tomography, Magnetic Resonance Imaging

    Pioglitazone (Pio) protects pancreatic β-cells and regulates blood glucose levels in high-fat (HF)-diet-induced diabetic mice. The pancreatic islet from KK-Ay mice, which were fed an HF diet with or without Pio, were examined by double-immunofluorescence for (A) insulin-activating transcription factor 6 and insulin-glucose-regulated protein 78 (GRP78), (B) insulin-monocyt e chemoattractant protein-1 and insulin-glucagon (GLU), (C) insulin-glucose transporter 2 (GLUT2). (A-C) The nucleus was visualized by 4′,6-diamidino-2-phenylindole (DAPI). Bars=20 µm. (D) Blood glucose levels and body weight were measured every week. INS, insulin; ATF6, activating transcription factor 6; MCP1, monocyte chemoattractant protein-1. a P

    Journal: Endocrinology and Metabolism

    Article Title: Pioglitazone Attenuates Palmitate-Induced Inflammation and Endoplasmic Reticulum Stress in Pancreatic β-Cells

    doi: 10.3803/EnM.2018.33.1.105

    Figure Lengend Snippet: Pioglitazone (Pio) protects pancreatic β-cells and regulates blood glucose levels in high-fat (HF)-diet-induced diabetic mice. The pancreatic islet from KK-Ay mice, which were fed an HF diet with or without Pio, were examined by double-immunofluorescence for (A) insulin-activating transcription factor 6 and insulin-glucose-regulated protein 78 (GRP78), (B) insulin-monocyt e chemoattractant protein-1 and insulin-glucagon (GLU), (C) insulin-glucose transporter 2 (GLUT2). (A-C) The nucleus was visualized by 4′,6-diamidino-2-phenylindole (DAPI). Bars=20 µm. (D) Blood glucose levels and body weight were measured every week. INS, insulin; ATF6, activating transcription factor 6; MCP1, monocyte chemoattractant protein-1. a P

    Article Snippet: After washing with phosphate-buffered saline, tissue samples were blocked using normal goat serum for 0.5 hours, and then incubated with primary antibodies for insulin (Millipore, Billerica, MA, USA), glucagon, Glut2, monocyte chemoattractant protein-1 (MCP1), ATF6α, and GRP78 (Santa Cruz Biotechnology) for 24 hours.

    Techniques: Mouse Assay, Immunofluorescence

    Quantitative polymerase chain reaction expression of ( A ) IL ‐6, ( B ) IL ‐15, ( C ) TNF ‐α, ( D ) NF ‐κB, ( E ) CRP , ( F ) E‐selectin ( CD 26E), ( G ) P‐selectin ( CD 26P), ( H ) ICAM ‐1. I, Western blot analysis showing expression of MCP ‐1. Representative Western blot was shown on the right and quantification on the left. Data were obtained from 3‐month‐old mouse hearts (n=6 mice/group) that were born to preconception FA or PM 2.5 exposed parents and expressed as mean±SEM. CRP indicates C‐reactive protein; ICAM1, intercellular adhesion molecule 1; IL‐6, interleukin 6; IL‐15, interleukin 15; MCP‐1, monocyte chemoattractant protein‐1; NF ‐κB, nuclear factor kappa‐light‐chain‐enhancer of activated B cells; PC FA, preconception filtered air; PC PM 2.5 , preconception particulate matter (

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Preconception Exposure to Fine Particulate Matter Leads to Cardiac Dysfunction in Adult Male Offspring

    doi: 10.1161/JAHA.118.010797

    Figure Lengend Snippet: Quantitative polymerase chain reaction expression of ( A ) IL ‐6, ( B ) IL ‐15, ( C ) TNF ‐α, ( D ) NF ‐κB, ( E ) CRP , ( F ) E‐selectin ( CD 26E), ( G ) P‐selectin ( CD 26P), ( H ) ICAM ‐1. I, Western blot analysis showing expression of MCP ‐1. Representative Western blot was shown on the right and quantification on the left. Data were obtained from 3‐month‐old mouse hearts (n=6 mice/group) that were born to preconception FA or PM 2.5 exposed parents and expressed as mean±SEM. CRP indicates C‐reactive protein; ICAM1, intercellular adhesion molecule 1; IL‐6, interleukin 6; IL‐15, interleukin 15; MCP‐1, monocyte chemoattractant protein‐1; NF ‐κB, nuclear factor kappa‐light‐chain‐enhancer of activated B cells; PC FA, preconception filtered air; PC PM 2.5 , preconception particulate matter (

    Article Snippet: Membranes were probed with antibodies to sarco/endoplasmic reticulum Ca2+ ‐ATPase (SERCA2a) (Thermo Fisher Scientific, MA3‐919, Waltham, MA), phosphorylated phospholamban (p‐PLN) (Santa Cruz Biotechnology, sc‐12963, Dallas, TX), Na+ /Ca2+ exchanger (NCX) (Thermo Fisher Scientific, MA3‐926, Waltham, MA), Nitric oxide synthase 2 (NOS2) (Santa Cruz Biotechnology, sc‐651, Dallas, TX), DNA methyltransferase 1 (DNMT1) (Abcam, ab‐13537, Cambridge, MA), DNA methyltransferase 3A (DNMT3A) (Abcam, ab‐13888, Cambridge, MA), monocyte chemoattractant protein‐1 (MCP‐1) (Santa Cruz Biotechnology, sc‐136750, Dallas, TX), and collagen type III alpha 1 chain (Santa Cruz Biotechnology, sc‐8780‐R, Dallas, TX).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Mouse Assay

    Arginase inhibition or EC-A1 deletion prevents HFHS-induced increased in expression of cytokines, chemokines and adhesion molecules in isolated aortic endothelial cells. Effects of ABH and EC-A1 deletion on protein levels of the proinflammatory cytokine tumor necrosis factor-α (TNF-α; A ), the chemokine monocyte chemoattractant protein-1 (MCP-1; B ), anti-inflammatory interleutin-10 (IL-10; C ), adhesion molecules vascular cell adhesion molecule-1 (VCAM-1; D ), and intercellular adhesion molecule 1 (ICAM; E ). Values are means ± SE; n = 6 mice. * P

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Obesity-induced vascular inflammation involves elevated arginase activity

    doi: 10.1152/ajpregu.00529.2016

    Figure Lengend Snippet: Arginase inhibition or EC-A1 deletion prevents HFHS-induced increased in expression of cytokines, chemokines and adhesion molecules in isolated aortic endothelial cells. Effects of ABH and EC-A1 deletion on protein levels of the proinflammatory cytokine tumor necrosis factor-α (TNF-α; A ), the chemokine monocyte chemoattractant protein-1 (MCP-1; B ), anti-inflammatory interleutin-10 (IL-10; C ), adhesion molecules vascular cell adhesion molecule-1 (VCAM-1; D ), and intercellular adhesion molecule 1 (ICAM; E ). Values are means ± SE; n = 6 mice. * P

    Article Snippet: Samples were assayed with the following ELISA kits: TNF-α (EMC102a; Neobioscience, Guangdong, China), monocyte chemoattractant protein-1 (MCP-1; EMC113; Neobioscience), IL-10 (EMC005; Neobioscience), ICAM1 (CD54; ab100688; Abcam), and VCAM1 (ab100750; Abcam).

    Techniques: Inhibition, Expressing, Isolation, Mouse Assay

    Release of cytokines by purified natural killer (NK) cells upon stimulation with selected expansion protocols. Purified NK cells were cultured using different stimulation protocols. On day 6, release of tumor necrosis factor (TNF)-α, IFN-γ, macrophage inflammatory protein (MIP)-1α, monocyte chemoattractant protein (MCP)-1, granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-8, and IL-10 were assayed by cytometric bead array analyses. Graphs show mean values and SDs obtained from four independent donors, except GM-CSF, which was measured for three independent donors. Asterisks indicate significant differences (* p

    Journal: Frontiers in Immunology

    Article Title: A Two-Phase Expansion Protocol Combining Interleukin (IL)-15 and IL-21 Improves Natural Killer Cell Proliferation and Cytotoxicity against Rhabdomyosarcoma

    doi: 10.3389/fimmu.2017.00676

    Figure Lengend Snippet: Release of cytokines by purified natural killer (NK) cells upon stimulation with selected expansion protocols. Purified NK cells were cultured using different stimulation protocols. On day 6, release of tumor necrosis factor (TNF)-α, IFN-γ, macrophage inflammatory protein (MIP)-1α, monocyte chemoattractant protein (MCP)-1, granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-8, and IL-10 were assayed by cytometric bead array analyses. Graphs show mean values and SDs obtained from four independent donors, except GM-CSF, which was measured for three independent donors. Asterisks indicate significant differences (* p

    Article Snippet: Cytometric Bead Array Cytokine secretion was examined by cytometric bead array analyses (CBA) on supernatants of stimulated NK cells on the sixth day of cultivation using BD CBA Flex Sets for IFN-γ, tumor necrosis factor (TNF)-α, macrophage inflammatory protein (MIP)-1α, monocyte chemoattractant protein (MCP)-1, IL-8, IL-10, and granulocyte-macrophage colony-stimulating factor (GM-CSF) (BD Biosciences).

    Techniques: Purification, Cell Culture

    Mast cells do not influence the production of interleukin-6 (IL-6), monocyte chemoattractant protein 1 (MCP-1), IL-3 and IL-13 in the peritoneum after Staphylococcus aureus infection. Mcpt5-Cre + × R-DTA and Mcpt5-Cre − × R-DTA were infected intraperitoneally with S. aureus . As a control, TSB medium only (bacterial growth medium) was injected. After 4 hr or 1 day, peritoneal lavage was performed. Peritoneal lavage fluids were analysed for levels of IL-6 (a), MCP-1 (b), IL-3 (c) and IL-13 (d) by ELISA. Mean ± SEM ( n = 5 to n = 11). * P

    Journal: Immunology

    Article Title: Mast cells are activated by Staphylococcus aureus in vitro but do not influence the outcome of intraperitoneal S. aureus infection in vivo

    doi: 10.1111/imm.12297

    Figure Lengend Snippet: Mast cells do not influence the production of interleukin-6 (IL-6), monocyte chemoattractant protein 1 (MCP-1), IL-3 and IL-13 in the peritoneum after Staphylococcus aureus infection. Mcpt5-Cre + × R-DTA and Mcpt5-Cre − × R-DTA were infected intraperitoneally with S. aureus . As a control, TSB medium only (bacterial growth medium) was injected. After 4 hr or 1 day, peritoneal lavage was performed. Peritoneal lavage fluids were analysed for levels of IL-6 (a), MCP-1 (b), IL-3 (c) and IL-13 (d) by ELISA. Mean ± SEM ( n = 5 to n = 11). * P

    Article Snippet: ELISAs for IL-3, IL-13, TNF- α (Peprotech, Rocky Hill, NJ), monocyte chemoattractant protein-1 (MCP-1) and IL-6 (eBioscience, San Diego, CA) were performed according to the manufacturer's instructions.

    Techniques: Infection, Injection, Enzyme-linked Immunosorbent Assay

    Immunofluorescence staining of MCP-1 and CCL22 in an area with a high degree of VSMC density. Fig. depict the same area as Fig. 1B- β . Immunofluorescence staining showed monocyte chemoattractant protein (MCP)-1 (clone 2.2-4H5-01A11, 10 µg/ml PeproTech Inc., Rocky Hill, NJ; red) positive cells were negative for CCL22 (green). Bars indicate 50 µm.

    Journal: Journal of Atherosclerosis and Thrombosis

    Article Title: Relationship between CCL22 Expression by Vascular Smooth Muscle Cells and Macrophage Histamine Receptors in Atherosclerosis

    doi: 10.5551/jat.44297

    Figure Lengend Snippet: Immunofluorescence staining of MCP-1 and CCL22 in an area with a high degree of VSMC density. Fig. depict the same area as Fig. 1B- β . Immunofluorescence staining showed monocyte chemoattractant protein (MCP)-1 (clone 2.2-4H5-01A11, 10 µg/ml PeproTech Inc., Rocky Hill, NJ; red) positive cells were negative for CCL22 (green). Bars indicate 50 µm.

    Article Snippet: Monoclonal anti-human CD68 (clone KP1, 1:100) and α SMA (clone 1A/4, 1:150) antibodies were obtained from Dako Japan, rabbit polyclonal antibodies to CCL22 (1:100) and monocyte chemoattractant protein (MCP)-1 (1:200) were obtained from Abcam (Tokyo, Japan), and monoclonal anti-human CD4 antibody (clone 4B12, undiluted) was obtained from Nichirei (Tokyo, Japan).

    Techniques: Immunofluorescence, Staining

    Cells surrounding macrophages in the intima Fig. A is the same area as Fig. 1B- β and Fig. B is the same area as 1B- γ . Fig. A MCP-1 shows monocyte chemoattractant protein (MCP)-1 immunohistochemical staining (green) and Fig. A MCP-1 and α -smooth muscle actin ( α SMA) shows immunofluorescence staining of both MCP-1 (green) and α SMA (red). MCP-1-positive cells and smooth muscle cells were present in close proximity. Fig. B CD4 and CD4 high-power magnification (high-power magnified image of the box in Fig. B CD4) show the immunohistochemical staining of CD4. Fig. B CCL22 and CD4 show immunofluorescence staining of both CCL22 (green) and CD4 (red). Fig. B CCR4 and interleukin (IL)-4 (high-power magnified image of Fig. B CCL22 and CD4) shows immunofluorescence staining of CCR4 (CCL22 receptor; green) and IL-4 (red). CD4-positive cells appeared around CCL22-positive cells whereas CD4-positive cells showed CCR4-staining and expressed IL-4. Blue staining (DAPI) indicates the nuclei of cells. Bars indicate 50 µm ( Fig. A), 100 µm ( Fig. B CD4) and 30 µm ( Fig. B CD4 [high-power magnification], CCL22 and CD4, CCR4, and IL-4).

    Journal: Journal of Atherosclerosis and Thrombosis

    Article Title: Relationship between CCL22 Expression by Vascular Smooth Muscle Cells and Macrophage Histamine Receptors in Atherosclerosis

    doi: 10.5551/jat.44297

    Figure Lengend Snippet: Cells surrounding macrophages in the intima Fig. A is the same area as Fig. 1B- β and Fig. B is the same area as 1B- γ . Fig. A MCP-1 shows monocyte chemoattractant protein (MCP)-1 immunohistochemical staining (green) and Fig. A MCP-1 and α -smooth muscle actin ( α SMA) shows immunofluorescence staining of both MCP-1 (green) and α SMA (red). MCP-1-positive cells and smooth muscle cells were present in close proximity. Fig. B CD4 and CD4 high-power magnification (high-power magnified image of the box in Fig. B CD4) show the immunohistochemical staining of CD4. Fig. B CCL22 and CD4 show immunofluorescence staining of both CCL22 (green) and CD4 (red). Fig. B CCR4 and interleukin (IL)-4 (high-power magnified image of Fig. B CCL22 and CD4) shows immunofluorescence staining of CCR4 (CCL22 receptor; green) and IL-4 (red). CD4-positive cells appeared around CCL22-positive cells whereas CD4-positive cells showed CCR4-staining and expressed IL-4. Blue staining (DAPI) indicates the nuclei of cells. Bars indicate 50 µm ( Fig. A), 100 µm ( Fig. B CD4) and 30 µm ( Fig. B CD4 [high-power magnification], CCL22 and CD4, CCR4, and IL-4).

    Article Snippet: Monoclonal anti-human CD68 (clone KP1, 1:100) and α SMA (clone 1A/4, 1:150) antibodies were obtained from Dako Japan, rabbit polyclonal antibodies to CCL22 (1:100) and monocyte chemoattractant protein (MCP)-1 (1:200) were obtained from Abcam (Tokyo, Japan), and monoclonal anti-human CD4 antibody (clone 4B12, undiluted) was obtained from Nichirei (Tokyo, Japan).

    Techniques: Immunohistochemistry, Staining, Immunofluorescence

    Monocyte chemoattractant protein 1 (MCP1) and haptoglobin (Hp) are affected in their capacity to migrate by CCR2 antagonist (RS1028959 exposition). Wound-healing assays were performed on SKOV-3 cells for 0, 12, 24 and 48 h at the indicated doses of Hp (250 μg) and monocyte chemoattractant protein 1 (MCP1, 250 ng). In addition, the effect of pretreatment with the chemokine (C-C motif) receptor 2 (CCR2) synthetic antagonist RS102895 (5 μM) on the capacity of SKOV-3 cells to migrate toward MCP1 (250 ng/ml) and Hp (0.250 mg/ml) is shown. As a negative control, bovine serum albumin (BSA) (1 mg/ml) was used. Chemotaxis was measured by confocal microscopy; nuclei (blue) were stained with DAPI (1:50). ( B ) Quantitative analysis of migration by the software Zen 2011 (Blue edition, Carl Zeiss) considering an average area of 9 × 10 5 μm 2 . This program determines the mean fluorescence intensity of each condition, which is plotted as a histogram. The results correspond to the average of 3 independent experiments. Bar scale = 100 μm.

    Journal: Cell Adhesion & Migration

    Article Title: Haptoglobin and CCR2 receptor expression in ovarian cancer cells that were exposed to ascitic fluid: Exploring a new role of haptoglobin in the tumoral microenvironment

    doi: 10.1080/19336918.2015.1035504

    Figure Lengend Snippet: Monocyte chemoattractant protein 1 (MCP1) and haptoglobin (Hp) are affected in their capacity to migrate by CCR2 antagonist (RS1028959 exposition). Wound-healing assays were performed on SKOV-3 cells for 0, 12, 24 and 48 h at the indicated doses of Hp (250 μg) and monocyte chemoattractant protein 1 (MCP1, 250 ng). In addition, the effect of pretreatment with the chemokine (C-C motif) receptor 2 (CCR2) synthetic antagonist RS102895 (5 μM) on the capacity of SKOV-3 cells to migrate toward MCP1 (250 ng/ml) and Hp (0.250 mg/ml) is shown. As a negative control, bovine serum albumin (BSA) (1 mg/ml) was used. Chemotaxis was measured by confocal microscopy; nuclei (blue) were stained with DAPI (1:50). ( B ) Quantitative analysis of migration by the software Zen 2011 (Blue edition, Carl Zeiss) considering an average area of 9 × 10 5 μm 2 . This program determines the mean fluorescence intensity of each condition, which is plotted as a histogram. The results correspond to the average of 3 independent experiments. Bar scale = 100 μm.

    Article Snippet: Finally, the medium was replaced with fresh complete medium, and the cells were incubated under different conditions: ascitic fluid from an ovarian cancer patient, 250 μg of Hp protein standard (Abcam, Burlingame, CA USA; Cat. No. ab77872) in culture media, 250 ng of monocyte chemoattractant protein 1 (MCP-1) (Abcam, Burlingame, CA USA; Cat. No. A9670) as a positive control, and 1 mg of bovine serum albumin (BSA) (Sigma Aldrich, St. Louis, MO; Cat. No. A9418) as a negative control.

    Techniques: Negative Control, Chemotaxis Assay, Confocal Microscopy, Staining, Migration, Software, Fluorescence

    Circulating inflammatory markers. Animals were fed standard chow (ST) or cafeteria diet (CAF) and trained 5 days per week for 30 min on a treadmill at different intensities (CON: 0; TML: 12; TMH: 17 m/min). Diets and training sessions were continued for 8 weeks. The serum levels of the cytokines monocyte chemoattractant protein-1 (MCP-1) and C-reactive protein (CRP) were determined at the end of the experiment. The data are given as the mean ± SEM (n = 9–12). D : the effect of diet; E : the effect of exercise (p

    Journal: PLoS ONE

    Article Title: Impact of a cafeteria diet and daily physical training on the rat serum metabolome

    doi: 10.1371/journal.pone.0171970

    Figure Lengend Snippet: Circulating inflammatory markers. Animals were fed standard chow (ST) or cafeteria diet (CAF) and trained 5 days per week for 30 min on a treadmill at different intensities (CON: 0; TML: 12; TMH: 17 m/min). Diets and training sessions were continued for 8 weeks. The serum levels of the cytokines monocyte chemoattractant protein-1 (MCP-1) and C-reactive protein (CRP) were determined at the end of the experiment. The data are given as the mean ± SEM (n = 9–12). D : the effect of diet; E : the effect of exercise (p

    Article Snippet: Leptin, adiponectin, C-reactive protein (CRP) (Millipore, Barcelona, Spain) and monocyte chemoattractant protein-1 (MCP-1) (Thermo Fisher Scientific, Pittsburgh, USA) were measured using rat ELISA kits.

    Techniques:

    SsnB administration modulates Kupffer cell activation in early steatohepatitic injury. A : qRT-PCR analysis of mRNA expression of CD68 and monocyte chemoattractant protein-1 (MCP1) from liver homogenates of S, SH, and SH+SsnB mice, normalized against S

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Sparstolonin B attenuates early liver inflammation in experimental NASH by modulating TLR4 trafficking in lipid rafts via NADPH oxidase activation

    doi: 10.1152/ajpgi.00259.2015

    Figure Lengend Snippet: SsnB administration modulates Kupffer cell activation in early steatohepatitic injury. A : qRT-PCR analysis of mRNA expression of CD68 and monocyte chemoattractant protein-1 (MCP1) from liver homogenates of S, SH, and SH+SsnB mice, normalized against S

    Article Snippet: Anti-mouse primary antibodies such as anti-IL-1β, anti-TNF-α, anti-monocyte chemoattractant protein-1 (MCP1), anti-F4/80, and anti-3-nitrotyrosine (Abcam, Cambridge, MA) were used in recommended dilutions.

    Techniques: Activation Assay, Quantitative RT-PCR, Expressing, Mouse Assay

    Anti-inflammatory effect of A. scholaris extract (ASE) on HaCaT human keratinocytes treated with all-trans retinoic acid (ATRA), as assessed by monitoring the protein expression of (a) monocyte chemoattractant protein-1 (MCP-1) and (b) interleukin-8 (IL-8). All results are expressed as average ± SD. * P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo

    doi: 10.1155/2012/190370

    Figure Lengend Snippet: Anti-inflammatory effect of A. scholaris extract (ASE) on HaCaT human keratinocytes treated with all-trans retinoic acid (ATRA), as assessed by monitoring the protein expression of (a) monocyte chemoattractant protein-1 (MCP-1) and (b) interleukin-8 (IL-8). All results are expressed as average ± SD. * P

    Article Snippet: Cells were treated with 50 μ L of diluted anti-irritants (madecassoside or hydrocortisone) or ASE for 10 min, then 50 μ L of 1 μ M ATRA in 1% FBS-containing media was added to the anti-irritant-treated cells, and incubation was continued for another 24 h. Supernatants were collected, and enzyme-linked immunosorbent assay (ELISA) was performed against interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) (BD OptEIATM; BD Biosciences, San Diego, Calif, USA) according to the manufacturer's protocol.

    Techniques: Expressing

    Receiver operating characteristic curves of Acute Physiology and Chronic Health (APACHE) II score, monocyte chemoattractant protein (MCP)-1 and a composite variable (APACHE II + MCP-1) predicting mortality. Shown are areas under receiver operating characteristic curves (AUROCs) for APACHE II score, monocyte MCP-1 and a composite variable (APACHE II + MCP-1), created according to the final model from multivariable analysis (Table 4).

    Journal: Critical Care

    Article Title: Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis

    doi: 10.1186/cc5783

    Figure Lengend Snippet: Receiver operating characteristic curves of Acute Physiology and Chronic Health (APACHE) II score, monocyte chemoattractant protein (MCP)-1 and a composite variable (APACHE II + MCP-1) predicting mortality. Shown are areas under receiver operating characteristic curves (AUROCs) for APACHE II score, monocyte MCP-1 and a composite variable (APACHE II + MCP-1), created according to the final model from multivariable analysis (Table 4).

    Article Snippet: A multiplex cytokine kit (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, IFN-γ, granulocyte colony-stimulating factor [G-CSF], granulocyte-macrophage colony-stimulating factor, monocyte chemoattractant protein [MCP]-1, macrophage inflammatory protein-1 and tumour necrosis factor [TNF]-α) was obtained and the assay performed in accordance with the manufacturer's instructions (Bio-Rad, Hercules, CA, USA).

    Techniques:

    Serial changes of MCP-1 expressions under IHC microscopic findings in kidney specimens. A. Illustrating the time intervals (i.e., 0, 6, 24 and 168 h) of cross section of IR kidney. B. Illustrating the immunohistochemical (IHC) stain (100x) for identification of monocyte chemoattractant protein (MCP)-1 expressions (gray color) in three different anatomical layers, including (1) inner stripe of outer medulla (ISOM), (2) outer stripe of outer medulla (OSOM) and (3) cortex in different time intervals (i.e., 0, 6, 24, 72 and 168 h) after acute kidney IR procedure. C. Analytical results of MCP-1 positively stained area in rat after acute kidney IR procedure: 1) ISOM layer, baseline vs. different time points (i.e., A, B, C), p

    Journal: American Journal of Translational Research

    Article Title: Extendin-4 protects kidney from acute ischemia-reperfusion injury through upregulation of NRF2 signaling

    doi:

    Figure Lengend Snippet: Serial changes of MCP-1 expressions under IHC microscopic findings in kidney specimens. A. Illustrating the time intervals (i.e., 0, 6, 24 and 168 h) of cross section of IR kidney. B. Illustrating the immunohistochemical (IHC) stain (100x) for identification of monocyte chemoattractant protein (MCP)-1 expressions (gray color) in three different anatomical layers, including (1) inner stripe of outer medulla (ISOM), (2) outer stripe of outer medulla (OSOM) and (3) cortex in different time intervals (i.e., 0, 6, 24, 72 and 168 h) after acute kidney IR procedure. C. Analytical results of MCP-1 positively stained area in rat after acute kidney IR procedure: 1) ISOM layer, baseline vs. different time points (i.e., A, B, C), p

    Article Snippet: Additionally, the paraffin kidney tissue block was sliced at 4 μm and immunohistochemically stained with the following antibody: monocyte chemoattractant protein-1 (MCP-1) (Cell Signaling).

    Techniques: Immunohistochemistry, Staining

    The expression of inflammatory cytokines and fibrotic factors are reduced in STZ-induced diabetic rats. ( A ) The kidney tissues were fixed in formalin and then subjected to immunofluorescence detection of TNF-α (arrow heads pointing to dark-brown dots indicating TNF-α expression). n = 5 per group, ( B ) IL6 protein level ( C ) MCP1 protein level ( D ) TGF-β mRNA level, ( E ) TGF-β protein level with a representative blot, ( F ) Fibronectin (FN) mRNA level in kidney tissues. Data are shown as the means ± SEM *p

    Journal: Aging (Albany NY)

    Article Title: Attenuation of diabetic kidney injury in DPP4-deficient rats; role of GLP-1 on the suppression of AGE formation by inducing glyoxalase 1

    doi: 10.18632/aging.102643

    Figure Lengend Snippet: The expression of inflammatory cytokines and fibrotic factors are reduced in STZ-induced diabetic rats. ( A ) The kidney tissues were fixed in formalin and then subjected to immunofluorescence detection of TNF-α (arrow heads pointing to dark-brown dots indicating TNF-α expression). n = 5 per group, ( B ) IL6 protein level ( C ) MCP1 protein level ( D ) TGF-β mRNA level, ( E ) TGF-β protein level with a representative blot, ( F ) Fibronectin (FN) mRNA level in kidney tissues. Data are shown as the means ± SEM *p

    Article Snippet: The antibodies used were as follows: anti-actin (#8457, Cell Signaling Technology, Danvers, MA, USA), anti-GAPDH (#MAB374, Millipore), anti-RAGE (sc-365154, Santa Cruz Biotechnology, Dallas, TX, USA), anti-DPP4/CD26 (5E8) (sc-8422, Santa Cruz), anti-glyoxalase-1 (sc-101537, Santa Cruz), anti-transforming growth factor (TGF)-β (#3711, Cell Signaling Technology), anti-Lamin A/C (#4777, Cell Signaling Technology, Danvers, MA, USA), anti-nuclear factor-erythroid 2 p45 subunit-related factor-2 (Nrf-2) (ab137550, Abcam), anti-monocyte chemoattractant protein 1 (MCP-1) (ab25124, Abcam), and anti-interleukin 6 (IL-6) (ab9324, Abcam).

    Techniques: Expressing, Immunofluorescence