monoclonal mouse anti mers cov nucleocapsid antibody Search Results


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  • 96
    Sino Biological mouse anti nucleocapsid
    Mouse Anti Nucleocapsid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti nucleocapsid/product/Sino Biological
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti nucleocapsid - by Bioz Stars, 2021-07
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    86
    Sino Biological monoclonal mouse anti mers cov nucleocapsid antibody
    Identification of an H2-d restricted T cell epitope in <t>MERS-CoV</t> N protein; ( a–d ) Groups of BALB/c mice ( n = 3 to 8) were vaccinated in a prime-boost regime with 10 8 PFU of MVA-MERS-N via i.p. ( a ) or i.m. ( b–d ) application. Mice immunized with non-recombinant MVA (MVA) and PBS served as negative controls. ( a-b ) Splenocytes were stimulated with individual 8-11-mer peptides and IFN-γ spot-forming CD8+ T cells (IFN-γ SFC) were measured by ELISPOT. ( c–d ) Splenocytes were stimulated with positive MERS-CoV N 10.2 peptide ( c ) or F2L 26-34 peptide ( d ) and IFN-γ producing CD8+ or CD4+ T cells were measured using intracellular cytokine staining assay and FACS analysis. The lines represent means. *
    Monoclonal Mouse Anti Mers Cov Nucleocapsid Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti mers cov nucleocapsid antibody/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse anti mers cov nucleocapsid antibody - by Bioz Stars, 2021-07
    86/100 stars
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    94
    BioVision sars cov 2 specific anti nucleocapsid mouse monoclonal antibody
    <t>SARS-CoV-2</t> RNA and antigen detection in bronchi. SARS-CoV-2 tropism in bronchi of mock (A and B) experimentally (C-H) infected cats determined by S-specific RNAscope ® in situ hybridization (Fast Red) and anti-N-specific immunohistochemistry (IHC; Fast Red). The viral tropism is limited to glandular and ductular epithelial cells of multifocal, scattered submucosal glands, as noted in the trachea. Viral RNA is detected within infected cells at 4 days post-challenge (DPC; C and D) and, to a lower degree at 7 DPC (E and F). Few scattered glandular epithelial cells are positive for SARS-CoV-2 N antigen by IHC (D and F, insets). No viral RNA or antigen is detected at 21 DPC (G and H). Total magnification: 100X (A, C, E and G) and 200X (B, D, F, H).
    Sars Cov 2 Specific Anti Nucleocapsid Mouse Monoclonal Antibody, supplied by BioVision, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 specific anti nucleocapsid mouse monoclonal antibody/product/BioVision
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 specific anti nucleocapsid mouse monoclonal antibody - by Bioz Stars, 2021-07
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    Identification of an H2-d restricted T cell epitope in MERS-CoV N protein; ( a–d ) Groups of BALB/c mice ( n = 3 to 8) were vaccinated in a prime-boost regime with 10 8 PFU of MVA-MERS-N via i.p. ( a ) or i.m. ( b–d ) application. Mice immunized with non-recombinant MVA (MVA) and PBS served as negative controls. ( a-b ) Splenocytes were stimulated with individual 8-11-mer peptides and IFN-γ spot-forming CD8+ T cells (IFN-γ SFC) were measured by ELISPOT. ( c–d ) Splenocytes were stimulated with positive MERS-CoV N 10.2 peptide ( c ) or F2L 26-34 peptide ( d ) and IFN-γ producing CD8+ or CD4+ T cells were measured using intracellular cytokine staining assay and FACS analysis. The lines represent means. *

    Journal: Viruses

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice

    doi: 10.3390/v10120718

    Figure Lengend Snippet: Identification of an H2-d restricted T cell epitope in MERS-CoV N protein; ( a–d ) Groups of BALB/c mice ( n = 3 to 8) were vaccinated in a prime-boost regime with 10 8 PFU of MVA-MERS-N via i.p. ( a ) or i.m. ( b–d ) application. Mice immunized with non-recombinant MVA (MVA) and PBS served as negative controls. ( a-b ) Splenocytes were stimulated with individual 8-11-mer peptides and IFN-γ spot-forming CD8+ T cells (IFN-γ SFC) were measured by ELISPOT. ( c–d ) Splenocytes were stimulated with positive MERS-CoV N 10.2 peptide ( c ) or F2L 26-34 peptide ( d ) and IFN-γ producing CD8+ or CD4+ T cells were measured using intracellular cytokine staining assay and FACS analysis. The lines represent means. *

    Article Snippet: After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies.

    Techniques: Mouse Assay, Recombinant, Enzyme-linked Immunospot, Staining, FACS

    Analysis of recombinant MVA-MERS proteins; ( a ) Western Blot analysis of MERS-CoV N protein produced in CEF or HaCat cells. Lysates from cells infected with recombinant MVA (MVA-MERS-N, MVA-MERS-S) or non-recombinant MVA (MVA) at a MOI of five, or from non-infected cells (mock) were prepared at eight, 12, or 24 hpi. Proteins were analyzed by immunoblotting with a monoclonal anti-MERS-N antibody; ( b – d ) Western Blot analysis of MERS-CoV N and S proteins produced in CEF. Total cell extracts from CEF infected with recombinant MVA (MVA-MERS-N, MVA-MERS-S) or non-recombinant MVA (MVA) at a MOI of five, or from non-infected cells (mock) were prepared at 24 hpi. Cell lysates and proteins were tested by immunoblotting using monoclonal anti MERS-N and anti MERS-S antibody ( b ) or polyclonal sera from MERS-CoV infected rabbits ( c ) or cynomolgus macaques ( d ). Arrows indicate the N- or S-specific protein bands.

    Journal: Viruses

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice

    doi: 10.3390/v10120718

    Figure Lengend Snippet: Analysis of recombinant MVA-MERS proteins; ( a ) Western Blot analysis of MERS-CoV N protein produced in CEF or HaCat cells. Lysates from cells infected with recombinant MVA (MVA-MERS-N, MVA-MERS-S) or non-recombinant MVA (MVA) at a MOI of five, or from non-infected cells (mock) were prepared at eight, 12, or 24 hpi. Proteins were analyzed by immunoblotting with a monoclonal anti-MERS-N antibody; ( b – d ) Western Blot analysis of MERS-CoV N and S proteins produced in CEF. Total cell extracts from CEF infected with recombinant MVA (MVA-MERS-N, MVA-MERS-S) or non-recombinant MVA (MVA) at a MOI of five, or from non-infected cells (mock) were prepared at 24 hpi. Cell lysates and proteins were tested by immunoblotting using monoclonal anti MERS-N and anti MERS-S antibody ( b ) or polyclonal sera from MERS-CoV infected rabbits ( c ) or cynomolgus macaques ( d ). Arrows indicate the N- or S-specific protein bands.

    Article Snippet: After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies.

    Techniques: Recombinant, Western Blot, Produced, Infection

    Screening for H2-d restricted T cell epitopes in MERS-CoV N protein using matrix peptide pools; ( a – b ) groups of BALB/c mice ( n = 2 to 6) were vaccinated twice (21-day interval) by i.p. ( a ) or i.m. ( b ) application with 10 8 plaque-forming-units (PFU) of recombinant MVA-MERS-N (MVA-N). Mice inoculated with non-recombinant MVA (MVA) or phosphate-buffered saline (PBS) were used as controls. Splenocytes were restimulated in vitro with pools of overlapping peptides corresponding to MERS-CoV N protein. IFN-γ spot-forming CD8+ T cells (IFN-γ SFC) were measured by ELISPOT. The lines represent means.

    Journal: Viruses

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice

    doi: 10.3390/v10120718

    Figure Lengend Snippet: Screening for H2-d restricted T cell epitopes in MERS-CoV N protein using matrix peptide pools; ( a – b ) groups of BALB/c mice ( n = 2 to 6) were vaccinated twice (21-day interval) by i.p. ( a ) or i.m. ( b ) application with 10 8 plaque-forming-units (PFU) of recombinant MVA-MERS-N (MVA-N). Mice inoculated with non-recombinant MVA (MVA) or phosphate-buffered saline (PBS) were used as controls. Splenocytes were restimulated in vitro with pools of overlapping peptides corresponding to MERS-CoV N protein. IFN-γ spot-forming CD8+ T cells (IFN-γ SFC) were measured by ELISPOT. The lines represent means.

    Article Snippet: After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies.

    Techniques: Mouse Assay, Recombinant, In Vitro, Enzyme-linked Immunospot

    SARS-CoV-2 RNA and antigen detection in bronchi. SARS-CoV-2 tropism in bronchi of mock (A and B) experimentally (C-H) infected cats determined by S-specific RNAscope ® in situ hybridization (Fast Red) and anti-N-specific immunohistochemistry (IHC; Fast Red). The viral tropism is limited to glandular and ductular epithelial cells of multifocal, scattered submucosal glands, as noted in the trachea. Viral RNA is detected within infected cells at 4 days post-challenge (DPC; C and D) and, to a lower degree at 7 DPC (E and F). Few scattered glandular epithelial cells are positive for SARS-CoV-2 N antigen by IHC (D and F, insets). No viral RNA or antigen is detected at 21 DPC (G and H). Total magnification: 100X (A, C, E and G) and 200X (B, D, F, H).

    Journal: bioRxiv

    Article Title: SARS-CoV-2 infection, disease and transmission in domestic cats

    doi: 10.1101/2020.08.04.235002

    Figure Lengend Snippet: SARS-CoV-2 RNA and antigen detection in bronchi. SARS-CoV-2 tropism in bronchi of mock (A and B) experimentally (C-H) infected cats determined by S-specific RNAscope ® in situ hybridization (Fast Red) and anti-N-specific immunohistochemistry (IHC; Fast Red). The viral tropism is limited to glandular and ductular epithelial cells of multifocal, scattered submucosal glands, as noted in the trachea. Viral RNA is detected within infected cells at 4 days post-challenge (DPC; C and D) and, to a lower degree at 7 DPC (E and F). Few scattered glandular epithelial cells are positive for SARS-CoV-2 N antigen by IHC (D and F, insets). No viral RNA or antigen is detected at 21 DPC (G and H). Total magnification: 100X (A, C, E and G) and 200X (B, D, F, H).

    Article Snippet: SARS-CoV-2-specific immunohistochemistry (IHC) For IHC, four-micron sections of formalin-fixed paraffin-embedded tissue were mounted on positively charged Superfrost® Plus slides and subjected to IHC using a SARS-CoV-2-specific anti-nucleocapsid mouse monoclonal antibody (clone 6F10, BioVision, Inc., Milpitas, CA, USA) as previously described ( ).

    Techniques: Infection, In Situ Hybridization, Immunohistochemistry

    Histopathology of bronchi. Histological findings in the main bronchi of mock (A) and SARS-CoV-2 experimentally infected (B-D) cats. Histologic changes and their progression are similar to those observed in the trachea, with multifocal, widespread, mild to moderate lymphocytic and neutrophilic adenitis noted at 4 days post-challenge (DPC; B) and 7 DPC (C). Necrotic debris within distorted submucosal glands are indicated with arrowheads (C), and few transmigrating lymphocytes are indicated with an arrow (B). No histologic changes are noted at 21 DPC (D). H E. Total magnification: 200X

    Journal: bioRxiv

    Article Title: SARS-CoV-2 infection, disease and transmission in domestic cats

    doi: 10.1101/2020.08.04.235002

    Figure Lengend Snippet: Histopathology of bronchi. Histological findings in the main bronchi of mock (A) and SARS-CoV-2 experimentally infected (B-D) cats. Histologic changes and their progression are similar to those observed in the trachea, with multifocal, widespread, mild to moderate lymphocytic and neutrophilic adenitis noted at 4 days post-challenge (DPC; B) and 7 DPC (C). Necrotic debris within distorted submucosal glands are indicated with arrowheads (C), and few transmigrating lymphocytes are indicated with an arrow (B). No histologic changes are noted at 21 DPC (D). H E. Total magnification: 200X

    Article Snippet: SARS-CoV-2-specific immunohistochemistry (IHC) For IHC, four-micron sections of formalin-fixed paraffin-embedded tissue were mounted on positively charged Superfrost® Plus slides and subjected to IHC using a SARS-CoV-2-specific anti-nucleocapsid mouse monoclonal antibody (clone 6F10, BioVision, Inc., Milpitas, CA, USA) as previously described ( ).

    Techniques: Histopathology, Infection

    Viral RNA in tissues. SARS-CoV-2 copy number (CN) per mg tissues based on N is shown. LN=lymph node; GI=gastrointestinal

    Journal: bioRxiv

    Article Title: SARS-CoV-2 infection, disease and transmission in domestic cats

    doi: 10.1101/2020.08.04.235002

    Figure Lengend Snippet: Viral RNA in tissues. SARS-CoV-2 copy number (CN) per mg tissues based on N is shown. LN=lymph node; GI=gastrointestinal

    Article Snippet: SARS-CoV-2-specific immunohistochemistry (IHC) For IHC, four-micron sections of formalin-fixed paraffin-embedded tissue were mounted on positively charged Superfrost® Plus slides and subjected to IHC using a SARS-CoV-2-specific anti-nucleocapsid mouse monoclonal antibody (clone 6F10, BioVision, Inc., Milpitas, CA, USA) as previously described ( ).

    Techniques:

    Study design. Ten cats were placed into three groups. Group 1 (principal infected animals) consisted of six cats (three cats/cage) and was inoculated via intranasal (IN) and oral (PO) routes simultaneously with a total dose of 1 × cephalic vein under anesthesia o 10 6 TCID 50 of SARS-CoV-2 in 2 ml DMEM. The cats in Group 2 (n=2; sentinel contact animals) and Group 3 (n=2; mock control animals) were housed in a separate room. At 1-day post challenge (DPC), the two cats in Group 2 were co-mingled with the principal infected animals in Group 1 (one cat per cage) and served as sentinel contact controls. The remaining two cats in Group 3 remained housed in a separate room and served as mock-infected negative controls. Principal infected animals were euthanized and necropsied at 4 (n=2), 7 (n=2) and 21 (n=1) DPC to evaluate the course of disease. The two negative control animals in Group 3 were euthanized and necropsied at 3 DPC. The remaining three animals from Group 1 (one principal infected animal) and Group 2 (two sentinel contact animals) were maintained for future re-infection studies.

    Journal: bioRxiv

    Article Title: SARS-CoV-2 infection, disease and transmission in domestic cats

    doi: 10.1101/2020.08.04.235002

    Figure Lengend Snippet: Study design. Ten cats were placed into three groups. Group 1 (principal infected animals) consisted of six cats (three cats/cage) and was inoculated via intranasal (IN) and oral (PO) routes simultaneously with a total dose of 1 × cephalic vein under anesthesia o 10 6 TCID 50 of SARS-CoV-2 in 2 ml DMEM. The cats in Group 2 (n=2; sentinel contact animals) and Group 3 (n=2; mock control animals) were housed in a separate room. At 1-day post challenge (DPC), the two cats in Group 2 were co-mingled with the principal infected animals in Group 1 (one cat per cage) and served as sentinel contact controls. The remaining two cats in Group 3 remained housed in a separate room and served as mock-infected negative controls. Principal infected animals were euthanized and necropsied at 4 (n=2), 7 (n=2) and 21 (n=1) DPC to evaluate the course of disease. The two negative control animals in Group 3 were euthanized and necropsied at 3 DPC. The remaining three animals from Group 1 (one principal infected animal) and Group 2 (two sentinel contact animals) were maintained for future re-infection studies.

    Article Snippet: SARS-CoV-2-specific immunohistochemistry (IHC) For IHC, four-micron sections of formalin-fixed paraffin-embedded tissue were mounted on positively charged Superfrost® Plus slides and subjected to IHC using a SARS-CoV-2-specific anti-nucleocapsid mouse monoclonal antibody (clone 6F10, BioVision, Inc., Milpitas, CA, USA) as previously described ( ).

    Techniques: Infection, Negative Control

    Felines shed SARS-CoV-2. RT-qPCR was performed on nasal (A), oropharyngeal (B) and rectal swabs (C) collected over the course of the 21-day study. Average viral copy number (CN) per mL with standard deviations are shown for each treatment group. Astrisks (*) indicate ½ of the sample RT-qPCR reactions were below the limit of detection.

    Journal: bioRxiv

    Article Title: SARS-CoV-2 infection, disease and transmission in domestic cats

    doi: 10.1101/2020.08.04.235002

    Figure Lengend Snippet: Felines shed SARS-CoV-2. RT-qPCR was performed on nasal (A), oropharyngeal (B) and rectal swabs (C) collected over the course of the 21-day study. Average viral copy number (CN) per mL with standard deviations are shown for each treatment group. Astrisks (*) indicate ½ of the sample RT-qPCR reactions were below the limit of detection.

    Article Snippet: SARS-CoV-2-specific immunohistochemistry (IHC) For IHC, four-micron sections of formalin-fixed paraffin-embedded tissue were mounted on positively charged Superfrost® Plus slides and subjected to IHC using a SARS-CoV-2-specific anti-nucleocapsid mouse monoclonal antibody (clone 6F10, BioVision, Inc., Milpitas, CA, USA) as previously described ( ).

    Techniques: Quantitative RT-PCR