Article Title: SARS-CoV-2 infection, disease and transmission in domestic cats
Figure Lengend Snippet: SARS-CoV-2 RNA and antigen detection in bronchi. SARS-CoV-2 tropism in bronchi of mock (A and B) experimentally (C-H) infected cats determined by S-specific RNAscope ® in situ hybridization (Fast Red) and anti-N-specific immunohistochemistry (IHC; Fast Red). The viral tropism is limited to glandular and ductular epithelial cells of multifocal, scattered submucosal glands, as noted in the trachea. Viral RNA is detected within infected cells at 4 days post-challenge (DPC; C and D) and, to a lower degree at 7 DPC (E and F). Few scattered glandular epithelial cells are positive for SARS-CoV-2 N antigen by IHC (D and F, insets). No viral RNA or antigen is detected at 21 DPC (G and H). Total magnification: 100X (A, C, E and G) and 200X (B, D, F, H).
Article Snippet: SARS-CoV-2-specific immunohistochemistry (IHC) For IHC, four-micron sections of formalin-fixed paraffin-embedded tissue were mounted on positively charged Superfrost® Plus slides and subjected to IHC using a SARS-CoV-2-specific anti-nucleocapsid mouse monoclonal antibody (clone 6F10, BioVision, Inc., Milpitas, CA, USA) as previously described ( ).
Techniques: Infection, In Situ Hybridization, Immunohistochemistry