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BioPorto Inc
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Quidel
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Image Search Results
Journal: The Journal of Immunology Author Choice
Article Title: Cholesterol Crystals Induce Coagulation Activation through Complement-Dependent Expression of Monocytic Tissue Factor
doi: 10.4049/jimmunol.1900503
Figure Lengend Snippet: Immunohistochemistry of frozen sections of human intracranial thrombus retracted from a patient with advanced carotid atherosclerosis and explored with polarization filter reflected light microscopy. (A and B) Staining against the monocyte marker CD14. (C) Staining against TF. (D) Staining against the complement neoepitopes C5b–9. (E) Staining against the complement neoepitope iC3b. The thicknesses of the sections are 4 μm. Pictures in the left column are taken by original magnification ×4 objective, and the CC-rich area (blue frame) is further augmented with an original magnification ×40 objective (middle and right columns). Examples of birefringent structures are shown by yellow arrows, whereas blue arrows point to the outer thrombi sections stained heavily for neoepitope iC3b. Scale bars, 200 μm for pictures in left column and 30 μm for pictures in middle and right columns.
Article Snippet: Abs for histology included
Techniques: Immunohistochemistry, Light Microscopy, Staining, Marker
Journal: The Journal of Immunology Author Choice
Article Title: Cholesterol Crystals Induce Coagulation Activation through Complement-Dependent Expression of Monocytic Tissue Factor
doi: 10.4049/jimmunol.1900503
Figure Lengend Snippet: Human vulnerable plaque and whole blood show corresponding patterns in monocyte TF/CD14 expression and complement activation to CC. (A) Immunohistochemistry of the vulnerable plaque with the monocyte marker CD14, TF, and activated complement neoepitope iC3b and neoepitopes C5b–9. Staining areas for CD14bright monocytes are marked with yellow arrows, and CD14low/TF+-expressing monocytes are marked by blue arrows; specified also are the CC-rich areas, lumen, and adventitia. Sections are 4 μm and are taken by original magnification ×4 objective and given for two adjacent areas in each staining. Scale bar, 200 μm. Whole blood following CC exposure showing effects on (B) monocyte CD14 expression, (C) correlation between CD14 and TF in monocytes, (D) the increase in monocyte granularity, (E) monocyte CD14 following inhibition of complement C3 (120 min), (F) monocyte CD14 following inhibition of complement C5 (120 min), (G) monocyte granularity following inhibition of C3 (120 min), and (H) monocyte granularity following inhibition of C5 (120 min). Data are given as mean fluorescence intensity (MFI; mean ± SEM, n = 6 donors). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Article Snippet: Abs for histology included
Techniques: Expressing, Activation Assay, Immunohistochemistry, Marker, Staining, Inhibition, Fluorescence