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  • 99
    Thermo Fisher monensin
    Monensin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monensin/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monensin - by Bioz Stars, 2021-03
    99/100 stars
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    86
    Becton Dickinson monensin
    PTEN is decreased in T cells from patients with SLE and correlates with SRSF1 levels. SRSF1 overexpression increases PTEN, suppresses mTORC1 activity, and reduces proinflammatory cytokine production. ( A and B ) Peripheral blood T cells were isolated from patients with SLE and normal (N) healthy control individuals. Total protein was immunoblotted for PTEN, SRSF1, and β-actin. ( A ) Data are from 1 representative of 10 independent experiments. Graph shows relative quantitation by densitometry ( n = 14 SRSF1 low and n = 12 SRSF1 normal patients). ( B ) Graph shows a linear correlation between relative PTEN and SRSF1 expression. ( C ) Peripheral blood T cells were isolated from patients with SLE and transfected with empty vector (EV) or SRSF1 overexpression plasmid (pSrsf1). At 16 to 18 hours after transfections, SLE T cells were stimulated with anti-CD3, anti-CD28, and crosslinker for 5 minutes. Total protein was immunoblotted for PTEN. Data are from 1 representative of 5 independent experiments ( n = 10). Graph shows relative quantitation by densitometry. ( D ) Total protein was immunoblotted for pS6 and total S6 in stimulated SLE T cells after transfection as described in C . Data are from 1 representative of 4 independent experiments ( n = 4). Graph shows relative quantitation by densitometry. ( E ) At 16 to 18 hours after transfection, cells were stimulated with PMA and ionomycin in the presence of <t>monensin.</t> After 4 hours, cytokine production was analyzed by flow cytometry. Graphs show quantitation of cytokine data ( n = 8). Paired t test, * P
    Monensin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monensin/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monensin - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    99
    Millipore monensin
    PTEN is decreased in T cells from patients with SLE and correlates with SRSF1 levels. SRSF1 overexpression increases PTEN, suppresses mTORC1 activity, and reduces proinflammatory cytokine production. ( A and B ) Peripheral blood T cells were isolated from patients with SLE and normal (N) healthy control individuals. Total protein was immunoblotted for PTEN, SRSF1, and β-actin. ( A ) Data are from 1 representative of 10 independent experiments. Graph shows relative quantitation by densitometry ( n = 14 SRSF1 low and n = 12 SRSF1 normal patients). ( B ) Graph shows a linear correlation between relative PTEN and SRSF1 expression. ( C ) Peripheral blood T cells were isolated from patients with SLE and transfected with empty vector (EV) or SRSF1 overexpression plasmid (pSrsf1). At 16 to 18 hours after transfections, SLE T cells were stimulated with anti-CD3, anti-CD28, and crosslinker for 5 minutes. Total protein was immunoblotted for PTEN. Data are from 1 representative of 5 independent experiments ( n = 10). Graph shows relative quantitation by densitometry. ( D ) Total protein was immunoblotted for pS6 and total S6 in stimulated SLE T cells after transfection as described in C . Data are from 1 representative of 4 independent experiments ( n = 4). Graph shows relative quantitation by densitometry. ( E ) At 16 to 18 hours after transfection, cells were stimulated with PMA and ionomycin in the presence of <t>monensin.</t> After 4 hours, cytokine production was analyzed by flow cytometry. Graphs show quantitation of cytokine data ( n = 8). Paired t test, * P
    Monensin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monensin/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monensin - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    99
    BioLegend monensin
    PTEN is decreased in T cells from patients with SLE and correlates with SRSF1 levels. SRSF1 overexpression increases PTEN, suppresses mTORC1 activity, and reduces proinflammatory cytokine production. ( A and B ) Peripheral blood T cells were isolated from patients with SLE and normal (N) healthy control individuals. Total protein was immunoblotted for PTEN, SRSF1, and β-actin. ( A ) Data are from 1 representative of 10 independent experiments. Graph shows relative quantitation by densitometry ( n = 14 SRSF1 low and n = 12 SRSF1 normal patients). ( B ) Graph shows a linear correlation between relative PTEN and SRSF1 expression. ( C ) Peripheral blood T cells were isolated from patients with SLE and transfected with empty vector (EV) or SRSF1 overexpression plasmid (pSrsf1). At 16 to 18 hours after transfections, SLE T cells were stimulated with anti-CD3, anti-CD28, and crosslinker for 5 minutes. Total protein was immunoblotted for PTEN. Data are from 1 representative of 5 independent experiments ( n = 10). Graph shows relative quantitation by densitometry. ( D ) Total protein was immunoblotted for pS6 and total S6 in stimulated SLE T cells after transfection as described in C . Data are from 1 representative of 4 independent experiments ( n = 4). Graph shows relative quantitation by densitometry. ( E ) At 16 to 18 hours after transfection, cells were stimulated with PMA and ionomycin in the presence of <t>monensin.</t> After 4 hours, cytokine production was analyzed by flow cytometry. Graphs show quantitation of cytokine data ( n = 8). Paired t test, * P
    Monensin, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monensin/product/BioLegend
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monensin - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    N/A
    Monensin is a naturally occuring ionophorous antibiotic that preferentially forms complexes with monovalent cations to enable transport across lipid membranes Through its ability to affect pH and the sodium potassium
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    N/A
    Monensin A is a polyether antibiotic first isolated from Streptomyces cinnamonensis in 1967 Monensin is a broad spectrum anticoccicidial antibiotic also exhibiting antifungal and antiviral activity Monensin A forms complexes
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    N/A
    Polyether ionophore autophagy inhibitor
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    N/A
    Monensin is a polyether ionophore antibiotic that blocks intracellular protein transport Monensin exhibits antibiotic antimalarial and other biological activities It is reported to inhibit the proliferation and induce apoptosis of
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    Image Search Results


    PTEN is decreased in T cells from patients with SLE and correlates with SRSF1 levels. SRSF1 overexpression increases PTEN, suppresses mTORC1 activity, and reduces proinflammatory cytokine production. ( A and B ) Peripheral blood T cells were isolated from patients with SLE and normal (N) healthy control individuals. Total protein was immunoblotted for PTEN, SRSF1, and β-actin. ( A ) Data are from 1 representative of 10 independent experiments. Graph shows relative quantitation by densitometry ( n = 14 SRSF1 low and n = 12 SRSF1 normal patients). ( B ) Graph shows a linear correlation between relative PTEN and SRSF1 expression. ( C ) Peripheral blood T cells were isolated from patients with SLE and transfected with empty vector (EV) or SRSF1 overexpression plasmid (pSrsf1). At 16 to 18 hours after transfections, SLE T cells were stimulated with anti-CD3, anti-CD28, and crosslinker for 5 minutes. Total protein was immunoblotted for PTEN. Data are from 1 representative of 5 independent experiments ( n = 10). Graph shows relative quantitation by densitometry. ( D ) Total protein was immunoblotted for pS6 and total S6 in stimulated SLE T cells after transfection as described in C . Data are from 1 representative of 4 independent experiments ( n = 4). Graph shows relative quantitation by densitometry. ( E ) At 16 to 18 hours after transfection, cells were stimulated with PMA and ionomycin in the presence of monensin. After 4 hours, cytokine production was analyzed by flow cytometry. Graphs show quantitation of cytokine data ( n = 8). Paired t test, * P

    Journal: The Journal of Clinical Investigation

    Article Title: Splicing factor SRSF1 controls T cell hyperactivity and systemic autoimmunity

    doi: 10.1172/JCI127949

    Figure Lengend Snippet: PTEN is decreased in T cells from patients with SLE and correlates with SRSF1 levels. SRSF1 overexpression increases PTEN, suppresses mTORC1 activity, and reduces proinflammatory cytokine production. ( A and B ) Peripheral blood T cells were isolated from patients with SLE and normal (N) healthy control individuals. Total protein was immunoblotted for PTEN, SRSF1, and β-actin. ( A ) Data are from 1 representative of 10 independent experiments. Graph shows relative quantitation by densitometry ( n = 14 SRSF1 low and n = 12 SRSF1 normal patients). ( B ) Graph shows a linear correlation between relative PTEN and SRSF1 expression. ( C ) Peripheral blood T cells were isolated from patients with SLE and transfected with empty vector (EV) or SRSF1 overexpression plasmid (pSrsf1). At 16 to 18 hours after transfections, SLE T cells were stimulated with anti-CD3, anti-CD28, and crosslinker for 5 minutes. Total protein was immunoblotted for PTEN. Data are from 1 representative of 5 independent experiments ( n = 10). Graph shows relative quantitation by densitometry. ( D ) Total protein was immunoblotted for pS6 and total S6 in stimulated SLE T cells after transfection as described in C . Data are from 1 representative of 4 independent experiments ( n = 4). Graph shows relative quantitation by densitometry. ( E ) At 16 to 18 hours after transfection, cells were stimulated with PMA and ionomycin in the presence of monensin. After 4 hours, cytokine production was analyzed by flow cytometry. Graphs show quantitation of cytokine data ( n = 8). Paired t test, * P

    Article Snippet: Anti–mouse Fas (clone Jo2) antibody, Cytofix/Cytoperm, Perm/Wash buffer, and monensin were from BD Biosciences.

    Techniques: Over Expression, Activity Assay, Isolation, Quantitation Assay, Expressing, Transfection, Plasmid Preparation, Flow Cytometry

    Rapamycin treatment reduces proinflammatory cytokine production by T cells and alleviates autoimmunity in Srsf1 -cKO mice. ( A – D ) Spleen cells from WT or Srsf1 -cKO mice were cultured for 4 hours with PMA plus ionomycin in the presence of monensin. Rapamycin (1 nM or 10 nM) was added for the duration of cultures. Cells were collected, surface stained, fixed, and permeabilized for intracellular cytokine staining. ( A and C ) Plots show IL-17 and IFN-γ intracellular staining gated on live CD4 + T cells. ( B ) Graphs show data from n = 8 mice in 7 independent experiments. ( D ) Graph shows data from n = 15 mice each in 10 independent experiments. ( E – H ) Rapamycin (2 mg/kg) or PBS was administered to WT and Srsf1 -cKO mice by intraperitoneal injection once every 2 days for 4 weeks ( F and G ) or every day for 1 week ( E and H ). ( E ) Spleen cells were cultured with anti-CD3 (2 μg/mL) and anti-CD28 (2 μg/mL) for 24 hours and IL-17A measured in the supernatants by ELISA. Data are shown as fold values normalized to controls ( n = 5 mice in 2 independent experiments). ( F ) Spleen cells were analyzed by flow cytometry, and graph shows the frequency of Tfh cells in spleen ( n = 5–6 mice in 2 independent experiments). ( G ) Serum was collected and autoantibodies measured by ELISA ( n = 5–6 mice in 2 independent experiments). ( H ) Cells from kidneys were analyzed by flow cytometry, and graph shows the frequency of T cells in kidneys ( n = 5 mice in 2 independent experiments). One-way ANOVA with Bonferroni correction ( B , D , E , and F ); 2-tailed unpaired t test ( G and H ); * P

    Journal: The Journal of Clinical Investigation

    Article Title: Splicing factor SRSF1 controls T cell hyperactivity and systemic autoimmunity

    doi: 10.1172/JCI127949

    Figure Lengend Snippet: Rapamycin treatment reduces proinflammatory cytokine production by T cells and alleviates autoimmunity in Srsf1 -cKO mice. ( A – D ) Spleen cells from WT or Srsf1 -cKO mice were cultured for 4 hours with PMA plus ionomycin in the presence of monensin. Rapamycin (1 nM or 10 nM) was added for the duration of cultures. Cells were collected, surface stained, fixed, and permeabilized for intracellular cytokine staining. ( A and C ) Plots show IL-17 and IFN-γ intracellular staining gated on live CD4 + T cells. ( B ) Graphs show data from n = 8 mice in 7 independent experiments. ( D ) Graph shows data from n = 15 mice each in 10 independent experiments. ( E – H ) Rapamycin (2 mg/kg) or PBS was administered to WT and Srsf1 -cKO mice by intraperitoneal injection once every 2 days for 4 weeks ( F and G ) or every day for 1 week ( E and H ). ( E ) Spleen cells were cultured with anti-CD3 (2 μg/mL) and anti-CD28 (2 μg/mL) for 24 hours and IL-17A measured in the supernatants by ELISA. Data are shown as fold values normalized to controls ( n = 5 mice in 2 independent experiments). ( F ) Spleen cells were analyzed by flow cytometry, and graph shows the frequency of Tfh cells in spleen ( n = 5–6 mice in 2 independent experiments). ( G ) Serum was collected and autoantibodies measured by ELISA ( n = 5–6 mice in 2 independent experiments). ( H ) Cells from kidneys were analyzed by flow cytometry, and graph shows the frequency of T cells in kidneys ( n = 5 mice in 2 independent experiments). One-way ANOVA with Bonferroni correction ( B , D , E , and F ); 2-tailed unpaired t test ( G and H ); * P

    Article Snippet: Anti–mouse Fas (clone Jo2) antibody, Cytofix/Cytoperm, Perm/Wash buffer, and monensin were from BD Biosciences.

    Techniques: Mouse Assay, Cell Culture, Staining, Injection, Enzyme-linked Immunosorbent Assay, Flow Cytometry

    PTEN overexpression reduces proinflammatory cytokine production by T cells from Srsf1 -cKO mice. ( A ) T cells were isolated from spleens of WT or Srsf1 -cKO mice and transfected by electroporation with empty vector (EV) or PTEN overexpression plasmid (pPTEN). Sixteen hours later, cells were stimulated with PMA plus ionomycin in the presence of monensin for 4 hours and cells were collected, surface stained, fixed, and permeabilized for intracellular cytokine staining. ( B ) Graphs show average data from n = 9 mice in 5 independent experiments. Two-tailed paired t test, * P

    Journal: The Journal of Clinical Investigation

    Article Title: Splicing factor SRSF1 controls T cell hyperactivity and systemic autoimmunity

    doi: 10.1172/JCI127949

    Figure Lengend Snippet: PTEN overexpression reduces proinflammatory cytokine production by T cells from Srsf1 -cKO mice. ( A ) T cells were isolated from spleens of WT or Srsf1 -cKO mice and transfected by electroporation with empty vector (EV) or PTEN overexpression plasmid (pPTEN). Sixteen hours later, cells were stimulated with PMA plus ionomycin in the presence of monensin for 4 hours and cells were collected, surface stained, fixed, and permeabilized for intracellular cytokine staining. ( B ) Graphs show average data from n = 9 mice in 5 independent experiments. Two-tailed paired t test, * P

    Article Snippet: Anti–mouse Fas (clone Jo2) antibody, Cytofix/Cytoperm, Perm/Wash buffer, and monensin were from BD Biosciences.

    Techniques: Over Expression, Mouse Assay, Isolation, Transfection, Electroporation, Plasmid Preparation, Staining, Two Tailed Test