moloney murine leukemia virus reverse transcriptase system Search Results


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  • 99
    Thermo Fisher moloney murine leukemia virus reverse transcriptase
    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with <t>Moloney</t> murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14759 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher moloney murine leukemia virus
    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with <t>Moloney</t> murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.
    Moloney Murine Leukemia Virus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega murine moloney leukemia virus
    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with <t>Moloney</t> murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.
    Murine Moloney Leukemia Virus, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 10375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Valiant moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GE Healthcare moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Valiant moloney murine leukemia virus
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Toyobo moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega moloney murine leukemia virus rna reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Rna Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bioteke Corporation moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eurobio moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Eurobio, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bioneer Corporation moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 93/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Bioneer Corporation
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    Price from $9.99 to $1999.99
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    Vazyme Biotech Co moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Vazyme Biotech Co
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    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-07
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    92
    Progen Biotechnik moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Progen Biotechnik
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    Image Search Results


    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with Moloney murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.

    Journal: Infection and Immunity

    Article Title: Phagocytosis of the Malarial Pigment, Hemozoin, Impairs Expression of Major Histocompatibility Complex Class II Antigen, CD54, and CD11c in Human Monocytes

    doi:

    Figure Lengend Snippet: Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with Moloney murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.

    Article Snippet: The cDNA synthesis from 15 ng of total cellular RNA from each extract was performed with 25 ng of random primers (Gibco BRL), 200 U of Moloney murine leukemia virus reverse transcriptase (Gibco BRL), and 20 U of RNase inhibitor (Boehringer Mannheim).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency *

    doi: 10.1074/jbc.M112.403881

    Figure Lengend Snippet: Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p

    Article Snippet: About 1.5 μg of RNA was used to synthesize cDNA using Random Hexamer Primer and Moloney murine leukemia virus reverse transcriptase (Promega) according to the manufacturer's protocol.

    Techniques: Transduction, Concentration Assay, Clone Assay, Real-time Polymerase Chain Reaction, Expressing, Staining, FACS, Infection