moloney murine leukemia virus reverse transcriptase Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs m mulv reverse transcriptase
    Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C <t>(M−MuLV</t> reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart <t>RTx</t> Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p
    M Mulv Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mulv reverse transcriptase/product/New England Biolabs
    Average 99 stars, based on 5210 article reviews
    Price from $9.99 to $1999.99
    m mulv reverse transcriptase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher moloney murine leukemia virus reverse transcriptase
    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with <t>Moloney</t> murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 14939 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    94
    Promega moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 10370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Promega
    Average 94 stars, based on 10370 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    93
    Thermo Fisher moloney murine leukaemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukaemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukaemia virus reverse transcriptase/product/Thermo Fisher
    Average 93 stars, based on 1240 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukaemia virus reverse transcriptase - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    Promega moloney murine leukemia virus m mlv reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus M Mlv Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus m mlv reverse transcriptase/product/Promega
    Average 99 stars, based on 1870 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus m mlv reverse transcriptase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher revertaid m mulv reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Revertaid M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid m mulv reverse transcriptase/product/Thermo Fisher
    Average 92 stars, based on 1832 article reviews
    Price from $9.99 to $1999.99
    revertaid m mulv reverse transcriptase - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Promega moloney murine leukemia virus
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 1893 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus/product/Promega
    Average 93 stars, based on 1893 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    92
    Thermo Fisher moloney murine leukemia virus
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus/product/Thermo Fisher
    Average 92 stars, based on 2334 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Thermo Fisher revertaid h minus m mulv reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Revertaid H Minus M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid h minus m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 1495 article reviews
    Price from $9.99 to $1999.99
    revertaid h minus m mulv reverse transcriptase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Promega moloney murine leukaemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukaemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukaemia virus reverse transcriptase/product/Promega
    Average 92 stars, based on 687 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukaemia virus reverse transcriptase - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    TaKaRa moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 893 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/TaKaRa
    Average 99 stars, based on 893 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore m mlv reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    M Mlv Reverse Transcriptase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 717 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv reverse transcriptase/product/Millipore
    Average 99 stars, based on 717 article reviews
    Price from $9.99 to $1999.99
    m mlv reverse transcriptase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher moloney murine leukemia virus reverse transcriptase kit
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase kit/product/Thermo Fisher
    Average 90 stars, based on 480 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase kit - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    92
    Stratagene moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Stratagene
    Average 92 stars, based on 251 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    94
    Millipore avian rt first strand synthesis kit
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Avian Rt First Strand Synthesis Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian rt first strand synthesis kit/product/Millipore
    Average 94 stars, based on 280 article reviews
    Price from $9.99 to $1999.99
    avian rt first strand synthesis kit - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher revert aid m mulv reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Revert Aid M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revert aid m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 356 article reviews
    Price from $9.99 to $1999.99
    revert aid m mulv reverse transcriptase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C (M−MuLV reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart RTx Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p

    Journal: Journal of Advanced Research

    Article Title: A reverse transcriptase-mediated ribosomal RNA depletion (RTR2D) strategy for the cost-effective construction of RNA sequencing libraries

    doi: 10.1016/j.jare.2019.12.005

    Figure Lengend Snippet: Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C (M−MuLV reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart RTx Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p

    Article Snippet: M−MuLV reverse transcriptase, ProtoScript® II Reverse Transcriptase, WarmStart RTx Reverse Transcriptase, RNase H, DNase I, Exonuclease I, Murine RNase Inhibitor, NEBNext® rRNA Depletion Kit (Human/Mouse/Rat), and NEBNext Ultra Directional RNA Library Prep Kit for Illumina were purchased from New England Biolabs (NEB, Ipswich, MA).

    Techniques: Expressing

    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with Moloney murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.

    Journal: Infection and Immunity

    Article Title: Phagocytosis of the Malarial Pigment, Hemozoin, Impairs Expression of Major Histocompatibility Complex Class II Antigen, CD54, and CD11c in Human Monocytes

    doi:

    Figure Lengend Snippet: Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with Moloney murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.

    Article Snippet: The cDNA synthesis from 15 ng of total cellular RNA from each extract was performed with 25 ng of random primers (Gibco BRL), 200 U of Moloney murine leukemia virus reverse transcriptase (Gibco BRL), and 20 U of RNase inhibitor (Boehringer Mannheim).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency *

    doi: 10.1074/jbc.M112.403881

    Figure Lengend Snippet: Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p

    Article Snippet: About 1.5 μg of RNA was used to synthesize cDNA using Random Hexamer Primer and Moloney murine leukemia virus reverse transcriptase (Promega) according to the manufacturer's protocol.

    Techniques: Transduction, Concentration Assay, Clone Assay, Real-time Polymerase Chain Reaction, Expressing, Staining, FACS, Infection