modulation by pctx1 Search Results


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  • 86
    Abcam spider venompeptide pctx 1
    Inhibition of ASIC1a improves acid-induced activation of HSCs. (A) Western blotting was used to detect the expression of collagen-1, ASIC1a and α -SMA in HSCs stimulated by pH 6.0. (B) qRT-PCR was used to detect the mRNA levels of collagen-1, ASIC1a, and α -SMA in HSCs stimulated by pH 6.0. (C) The effect of <t>PcTx-1</t> on the viability of HSCs was detected by CCK8. (D) Western blotting was used to detect the expression of collagen-1, α -SMA, and ASIC1a in HSCs under the intervention of PcTx-1. (E) qRT-PCR to detect the expression of collagen-1, ASIC1a, and α -SMA under the intervention of PcTx-1. (F) Immunofluorescence to detect the effect of PcTx-1, a specific blocker of ASIC1a channel, on ASIC1a in HSCs. (Scale Bar, 50 μM). Data are expressed as the mean ± SD ( n ≥ 3). * p < .05, ** p < .01 vs. Control group; # p < .05, ## p < .01 vs. pH 6.0 group.
    Spider Venompeptide Pctx 1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Merck KGaA pctx 1
    ( A ) Time evolution of current noise of C6 glioma cells upon adding <t>PcTX-1,</t> up to a concentration of 100 nM in acidified cell culture medium. The black line represents the original state. The red line is recorded after adding PcTX-1 to a concentration of only 100 nM. The blue curve represents the recovery plot after thrice washing. ( B ) The corresponding current noise spectra of . ( C ) The mean of 300 spike amplitudes of acidified cells as depicted in . PcTX-1 data quantification recorded over two inhibitory experiments.
    Pctx 1, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pctx 1/product/Merck KGaA
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    86
    Peptide Institute pctx1
    ( a – d ) Pharmacological profiles of the light (530–550 nm filter)-induced ASIC-like currents in single HEK293T cells co-expressing ASIC1a-GFP and Arch-mCherry. Amiloride (Ami), 100 μM; N-methyl-D-glucamine (NMDG), 150 mM; <t>PcTx1,</t> 100 nM. ( a – c) representative traces. ( d ) Summary for various pharmacological effects. Data represent means ± SEM (*** p < 0.001 by paired t test, n = 8, 10, and 6 for Ami, NMDG, and PcTx1, respectively). ( e ) Light stimulation of single neurons that co-expressed ASIC1a-GFP and Arch-mCherry induced ASIC-like inward currents. Treatment with Ami (100 μM, red bar) during light stimulation effectively blocked the inward current component. The pH 6.0 (black bar)-induced current was used as a positive control. ( f ) Light failed to induce any detectable inward currents in single HEK293T cells co-expressing the nonconducting ASIC1a mutant 32 HIF 34–32 AAA 34 (HIF)-GFP and Arch-mCherry (0/8 cells). ( g ) ASIC1a mutant-GFP has a normal surface expression level compared with ASIC1a WT-GFP control. Surface proteins were determined by surface biotinylation assay. Data represent means ± SEM (N.S., not significant, paired t test, n = 3).
    Pctx1, supplied by Peptide Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore pctx1
    Comparison of putative functional dyads of <t>PcTx1</t> (A) and related ICK toxins J-atracotoxin Hv1c (PDB 1DL0; B), κ-conotoxin PVIIA (PDB 1KCP; C), and agitoxin 2 (PDB 1AGT; D). Basic residues side chains are colored in blue; aromatic residues side chains, in orange.
    Pctx1, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Spider Pharm Inc pctx1
    a , Current-voltage relationships of MitTx (300 nM)-evoked conductances from TG neurons (whole-cell configuration) demonstrate higher permeability for Na + over Cs + . The intracellular solution contained 150 mM Na + , and a leftward-shift in the reversal potential was observed when the major extracellular cation was changed from 150 mM Na + to 150 mM Cs + . b , Voltage clamp recordings show that ASIC1b-expressing oocytes respond to both extracellular acidification (H + , pH 4) and MitTx, but are insensitive to MitTxα (30 nM) or MitTxβ (300 nM) individually. Toxin-evoked responses were blocked by amiloride (Amil, 1mM). c , MitTx (75 nM)-evoked currents are comparable in magnitude to pH 4-evoked currents in ASIC1b-expressing oocytes. Toxin responses are non-desensitizing and persistent compared to transient proton-evoked currents. d , Dose-response analysis of toxin-evoked currents normalized to maximal pH 4-evoked response in ASIC-expressing oocytes. Data were fit to the Hill equation. e , MitTx (75 nM) is a poor ASIC2a agonist, but dramatically potentiates pH 5.5-evoked responses. f , pH dose-response of ASIC2a in the absence (dark green) or presence (light green) of 75 nM MitTx. Data were fit to the Hill equation. g , <t>PcTx1</t> (100 nM) inhibits both pH 6- and MitTx-evoked currents in ASIC1a-expressing oocytes. h , MitTx occludes PcTx1 inhibition. Vertical scale bars: 1μA; horizontal bars: 1 min; V h = -60 mV.
    Pctx1, supplied by Spider Pharm Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inhibition of ASIC1a improves acid-induced activation of HSCs. (A) Western blotting was used to detect the expression of collagen-1, ASIC1a and α -SMA in HSCs stimulated by pH 6.0. (B) qRT-PCR was used to detect the mRNA levels of collagen-1, ASIC1a, and α -SMA in HSCs stimulated by pH 6.0. (C) The effect of PcTx-1 on the viability of HSCs was detected by CCK8. (D) Western blotting was used to detect the expression of collagen-1, α -SMA, and ASIC1a in HSCs under the intervention of PcTx-1. (E) qRT-PCR to detect the expression of collagen-1, ASIC1a, and α -SMA under the intervention of PcTx-1. (F) Immunofluorescence to detect the effect of PcTx-1, a specific blocker of ASIC1a channel, on ASIC1a in HSCs. (Scale Bar, 50 μM). Data are expressed as the mean ± SD ( n ≥ 3). * p < .05, ** p < .01 vs. Control group; # p < .05, ## p < .01 vs. pH 6.0 group.

    Journal: Frontiers in Pharmacology

    Article Title: CaM/CaMKII mediates activation and proliferation of hepatic stellate cells regulated by ASIC1a

    doi: 10.3389/fphar.2022.996667

    Figure Lengend Snippet: Inhibition of ASIC1a improves acid-induced activation of HSCs. (A) Western blotting was used to detect the expression of collagen-1, ASIC1a and α -SMA in HSCs stimulated by pH 6.0. (B) qRT-PCR was used to detect the mRNA levels of collagen-1, ASIC1a, and α -SMA in HSCs stimulated by pH 6.0. (C) The effect of PcTx-1 on the viability of HSCs was detected by CCK8. (D) Western blotting was used to detect the expression of collagen-1, α -SMA, and ASIC1a in HSCs under the intervention of PcTx-1. (E) qRT-PCR to detect the expression of collagen-1, ASIC1a, and α -SMA under the intervention of PcTx-1. (F) Immunofluorescence to detect the effect of PcTx-1, a specific blocker of ASIC1a channel, on ASIC1a in HSCs. (Scale Bar, 50 μM). Data are expressed as the mean ± SD ( n ≥ 3). * p < .05, ** p < .01 vs. Control group; # p < .05, ## p < .01 vs. pH 6.0 group.

    Article Snippet: Spider-venompeptide (PcTx-1) was purchased from Abcam (Cambridge, United States).

    Techniques: Inhibition, Activation Assay, Western Blot, Expressing, Quantitative RT-PCR, Immunofluorescence

    Effect of ASIC1a-siRNA transfection and PcTx-1 on ASIC1a, α -SMA, and collagen-Ι as well as CaM and CaMKⅡ expression in HSC-T6 cells. (A) Western blotting was used to detect the expression of CaM and CaMKⅡ protein in HSCs stimulated by pH 6.0. (B) The mRNA levels of CaM and CaMKⅡ in HSCs stimulated by pH 6.0 were detected by qRT-PCR. (C) ASIC1a protein level in HSCs was detected by Western blotting after silencing ASIC1a. (D) ASIC1a mRNA level was detected by qRT-PCR after silencing ASIC1a. (E) Expression of CaM, CaMKII and fibrosis protein in HSCs after silencing ASIC1a. (F) mRNA level of CaM, CaMKII, and fibroprotein in HSCs after silencing ASIC1a. (G) CaM and CaMKⅡ protein levels in HSCs treated with PcTx-1 were detected by Western blotting. (H) The mRNA levels of CaM and CaMKⅡ in HSCs treated with PcTx-1 were detected by qRT-PCR. Statistical analyses were performed using t -test. Data are expressed as the mean ± SD ( n ≥ 3). * p < .05, ** p < .01 vs. Control group; # p < .05, ## p < .01 vs. pH 6.0 group.

    Journal: Frontiers in Pharmacology

    Article Title: CaM/CaMKII mediates activation and proliferation of hepatic stellate cells regulated by ASIC1a

    doi: 10.3389/fphar.2022.996667

    Figure Lengend Snippet: Effect of ASIC1a-siRNA transfection and PcTx-1 on ASIC1a, α -SMA, and collagen-Ι as well as CaM and CaMKⅡ expression in HSC-T6 cells. (A) Western blotting was used to detect the expression of CaM and CaMKⅡ protein in HSCs stimulated by pH 6.0. (B) The mRNA levels of CaM and CaMKⅡ in HSCs stimulated by pH 6.0 were detected by qRT-PCR. (C) ASIC1a protein level in HSCs was detected by Western blotting after silencing ASIC1a. (D) ASIC1a mRNA level was detected by qRT-PCR after silencing ASIC1a. (E) Expression of CaM, CaMKII and fibrosis protein in HSCs after silencing ASIC1a. (F) mRNA level of CaM, CaMKII, and fibroprotein in HSCs after silencing ASIC1a. (G) CaM and CaMKⅡ protein levels in HSCs treated with PcTx-1 were detected by Western blotting. (H) The mRNA levels of CaM and CaMKⅡ in HSCs treated with PcTx-1 were detected by qRT-PCR. Statistical analyses were performed using t -test. Data are expressed as the mean ± SD ( n ≥ 3). * p < .05, ** p < .01 vs. Control group; # p < .05, ## p < .01 vs. pH 6.0 group.

    Article Snippet: Spider-venompeptide (PcTx-1) was purchased from Abcam (Cambridge, United States).

    Techniques: Transfection, Expressing, Western Blot, Quantitative RT-PCR

    ( A ) Time evolution of current noise of C6 glioma cells upon adding PcTX-1, up to a concentration of 100 nM in acidified cell culture medium. The black line represents the original state. The red line is recorded after adding PcTX-1 to a concentration of only 100 nM. The blue curve represents the recovery plot after thrice washing. ( B ) The corresponding current noise spectra of . ( C ) The mean of 300 spike amplitudes of acidified cells as depicted in . PcTX-1 data quantification recorded over two inhibitory experiments.

    Journal: Science Advances

    Article Title: Extracellular electrical recording of pH-triggered bursts in C6 glioma cell populations

    doi: 10.1126/sciadv.1600516

    Figure Lengend Snippet: ( A ) Time evolution of current noise of C6 glioma cells upon adding PcTX-1, up to a concentration of 100 nM in acidified cell culture medium. The black line represents the original state. The red line is recorded after adding PcTX-1 to a concentration of only 100 nM. The blue curve represents the recovery plot after thrice washing. ( B ) The corresponding current noise spectra of . ( C ) The mean of 300 spike amplitudes of acidified cells as depicted in . PcTX-1 data quantification recorded over two inhibitory experiments.

    Article Snippet: PcTX-1 (Merck Chemicals GmbH) was directly dissolved in cell culture medium (F-12K) to a working concentration of 0.1 M. Final dilutions of 1 μM TTX and 100 nM PcTX-1 were obtained by diluting the working solution in the cell culture medium.

    Techniques: Concentration Assay, Cell Culture

    ( a – d ) Pharmacological profiles of the light (530–550 nm filter)-induced ASIC-like currents in single HEK293T cells co-expressing ASIC1a-GFP and Arch-mCherry. Amiloride (Ami), 100 μM; N-methyl-D-glucamine (NMDG), 150 mM; PcTx1, 100 nM. ( a – c) representative traces. ( d ) Summary for various pharmacological effects. Data represent means ± SEM (*** p < 0.001 by paired t test, n = 8, 10, and 6 for Ami, NMDG, and PcTx1, respectively). ( e ) Light stimulation of single neurons that co-expressed ASIC1a-GFP and Arch-mCherry induced ASIC-like inward currents. Treatment with Ami (100 μM, red bar) during light stimulation effectively blocked the inward current component. The pH 6.0 (black bar)-induced current was used as a positive control. ( f ) Light failed to induce any detectable inward currents in single HEK293T cells co-expressing the nonconducting ASIC1a mutant 32 HIF 34–32 AAA 34 (HIF)-GFP and Arch-mCherry (0/8 cells). ( g ) ASIC1a mutant-GFP has a normal surface expression level compared with ASIC1a WT-GFP control. Surface proteins were determined by surface biotinylation assay. Data represent means ± SEM (N.S., not significant, paired t test, n = 3).

    Journal: Scientific Reports

    Article Title: Activation of acid-sensing ion channels by localized proton transient reveals their role in proton signaling

    doi: 10.1038/srep14125

    Figure Lengend Snippet: ( a – d ) Pharmacological profiles of the light (530–550 nm filter)-induced ASIC-like currents in single HEK293T cells co-expressing ASIC1a-GFP and Arch-mCherry. Amiloride (Ami), 100 μM; N-methyl-D-glucamine (NMDG), 150 mM; PcTx1, 100 nM. ( a – c) representative traces. ( d ) Summary for various pharmacological effects. Data represent means ± SEM (*** p < 0.001 by paired t test, n = 8, 10, and 6 for Ami, NMDG, and PcTx1, respectively). ( e ) Light stimulation of single neurons that co-expressed ASIC1a-GFP and Arch-mCherry induced ASIC-like inward currents. Treatment with Ami (100 μM, red bar) during light stimulation effectively blocked the inward current component. The pH 6.0 (black bar)-induced current was used as a positive control. ( f ) Light failed to induce any detectable inward currents in single HEK293T cells co-expressing the nonconducting ASIC1a mutant 32 HIF 34–32 AAA 34 (HIF)-GFP and Arch-mCherry (0/8 cells). ( g ) ASIC1a mutant-GFP has a normal surface expression level compared with ASIC1a WT-GFP control. Surface proteins were determined by surface biotinylation assay. Data represent means ± SEM (N.S., not significant, paired t test, n = 3).

    Article Snippet: PcTX1 was purchased from the Peptide Institute.

    Techniques: Expressing, Positive Control, Mutagenesis, Surface Biotinylation Assay

    Comparison of putative functional dyads of PcTx1 (A) and related ICK toxins J-atracotoxin Hv1c (PDB 1DL0; B), κ-conotoxin PVIIA (PDB 1KCP; C), and agitoxin 2 (PDB 1AGT; D). Basic residues side chains are colored in blue; aromatic residues side chains, in orange.

    Journal:

    Article Title: Recombinant production and solution structure of PcTx1, the specific peptide inhibitor of ASIC1a proton-gated cation channels

    doi:

    Figure Lengend Snippet: Comparison of putative functional dyads of PcTx1 (A) and related ICK toxins J-atracotoxin Hv1c (PDB 1DL0; B), κ-conotoxin PVIIA (PDB 1KCP; C), and agitoxin 2 (PDB 1AGT; D). Basic residues side chains are colored in blue; aromatic residues side chains, in orange.

    Article Snippet: For these assays, cells were plated at 3 × 10 6 cells/mL in 24-well plates and induced 1 d later with 500 μM CuSO 4 for 3 to 7 d. Fifty microliters of cell medium were loaded on a C18 ZipTip (Millipore) to remove salts, and the presence of PcTx1 was determined by MALDI-TOF mass spectrometry.

    Techniques: Functional Assay

    Purification of recombinant PcTx1. (A) Purification scheme. (B) Cation-exchange HPLC of PcTx1r after two steps of batch purification (HPLC step 1). Linear gradient of ammonium acetate, on a Tosoh SP5PW cation-exchange semipreparative column (70 × 4.6 mm). (C) Reversed-phase HPLC final purifcation of PcTx1r (HPLC step 2). Linear gradient of acetonitrile on a semipreparative C18 column. (D) Reflector MALDI-TOF spectrum of purified PcTx1r, internal calibration. Calculated monoisotopic m/z 4687.1824, measured 4687.1722 (accuracy 2.1 ppm).

    Journal:

    Article Title: Recombinant production and solution structure of PcTx1, the specific peptide inhibitor of ASIC1a proton-gated cation channels

    doi:

    Figure Lengend Snippet: Purification of recombinant PcTx1. (A) Purification scheme. (B) Cation-exchange HPLC of PcTx1r after two steps of batch purification (HPLC step 1). Linear gradient of ammonium acetate, on a Tosoh SP5PW cation-exchange semipreparative column (70 × 4.6 mm). (C) Reversed-phase HPLC final purifcation of PcTx1r (HPLC step 2). Linear gradient of acetonitrile on a semipreparative C18 column. (D) Reflector MALDI-TOF spectrum of purified PcTx1r, internal calibration. Calculated monoisotopic m/z 4687.1824, measured 4687.1722 (accuracy 2.1 ppm).

    Article Snippet: For these assays, cells were plated at 3 × 10 6 cells/mL in 24-well plates and induced 1 d later with 500 μM CuSO 4 for 3 to 7 d. Fifty microliters of cell medium were loaded on a C18 ZipTip (Millipore) to remove salts, and the presence of PcTx1 was determined by MALDI-TOF mass spectrometry.

    Techniques: Purification, Recombinant

    Sequence of PcTx1 and sequential assignment. Collected sequential NOEs are classified into strong and weak NOEs and are indicated by thick and thin bars, respectively. The secondary elements (β-strands) are indicated by arrows.

    Journal:

    Article Title: Recombinant production and solution structure of PcTx1, the specific peptide inhibitor of ASIC1a proton-gated cation channels

    doi:

    Figure Lengend Snippet: Sequence of PcTx1 and sequential assignment. Collected sequential NOEs are classified into strong and weak NOEs and are indicated by thick and thin bars, respectively. The secondary elements (β-strands) are indicated by arrows.

    Article Snippet: For these assays, cells were plated at 3 × 10 6 cells/mL in 24-well plates and induced 1 d later with 500 μM CuSO 4 for 3 to 7 d. Fifty microliters of cell medium were loaded on a C18 ZipTip (Millipore) to remove salts, and the presence of PcTx1 was determined by MALDI-TOF mass spectrometry.

    Techniques: Sequencing

    (A) Stereoview of the best fit of 20 solution structures of PcTx1. Cα are shown. The N and C termini are labeled

    Journal:

    Article Title: Recombinant production and solution structure of PcTx1, the specific peptide inhibitor of ASIC1a proton-gated cation channels

    doi:

    Figure Lengend Snippet: (A) Stereoview of the best fit of 20 solution structures of PcTx1. Cα are shown. The N and C termini are labeled "N" and "C". (B) Molecular surface colored according to the electrostatic charge (red for acidic and blue for basic) and the resulting dipolar moment (red arrow). The aromatic residues are indicated and colored in purple. (C) CPK representation of the proposed functional surface of PcTx1. The residues suspected to be important for the interaction of the toxin with the channel are labeled. Residues are colored as follows: green for polar uncharged residues, blue for basic residues, red for acidic residues, purple for aromatic residues, and yellow for aliphatic residues.

    Article Snippet: For these assays, cells were plated at 3 × 10 6 cells/mL in 24-well plates and induced 1 d later with 500 μM CuSO 4 for 3 to 7 d. Fifty microliters of cell medium were loaded on a C18 ZipTip (Millipore) to remove salts, and the presence of PcTx1 was determined by MALDI-TOF mass spectrometry.

    Techniques: Labeling, Functional Assay

    Comparison of three-dimensional structures of structurally related toxins blocking Na+ channels with PcTx1, in Molscript representation.

    Journal:

    Article Title: Recombinant production and solution structure of PcTx1, the specific peptide inhibitor of ASIC1a proton-gated cation channels

    doi:

    Figure Lengend Snippet: Comparison of three-dimensional structures of structurally related toxins blocking Na+ channels with PcTx1, in Molscript representation.

    Article Snippet: For these assays, cells were plated at 3 × 10 6 cells/mL in 24-well plates and induced 1 d later with 500 μM CuSO 4 for 3 to 7 d. Fifty microliters of cell medium were loaded on a C18 ZipTip (Millipore) to remove salts, and the presence of PcTx1 was determined by MALDI-TOF mass spectrometry.

    Techniques: Blocking Assay

    Structural statistics of the  PcTx1  20 best structures obtained by distance geometry and minimization

    Journal:

    Article Title: Recombinant production and solution structure of PcTx1, the specific peptide inhibitor of ASIC1a proton-gated cation channels

    doi:

    Figure Lengend Snippet: Structural statistics of the PcTx1 20 best structures obtained by distance geometry and minimization

    Article Snippet: For these assays, cells were plated at 3 × 10 6 cells/mL in 24-well plates and induced 1 d later with 500 μM CuSO 4 for 3 to 7 d. Fifty microliters of cell medium were loaded on a C18 ZipTip (Millipore) to remove salts, and the presence of PcTx1 was determined by MALDI-TOF mass spectrometry.

    Techniques:

    a , Current-voltage relationships of MitTx (300 nM)-evoked conductances from TG neurons (whole-cell configuration) demonstrate higher permeability for Na + over Cs + . The intracellular solution contained 150 mM Na + , and a leftward-shift in the reversal potential was observed when the major extracellular cation was changed from 150 mM Na + to 150 mM Cs + . b , Voltage clamp recordings show that ASIC1b-expressing oocytes respond to both extracellular acidification (H + , pH 4) and MitTx, but are insensitive to MitTxα (30 nM) or MitTxβ (300 nM) individually. Toxin-evoked responses were blocked by amiloride (Amil, 1mM). c , MitTx (75 nM)-evoked currents are comparable in magnitude to pH 4-evoked currents in ASIC1b-expressing oocytes. Toxin responses are non-desensitizing and persistent compared to transient proton-evoked currents. d , Dose-response analysis of toxin-evoked currents normalized to maximal pH 4-evoked response in ASIC-expressing oocytes. Data were fit to the Hill equation. e , MitTx (75 nM) is a poor ASIC2a agonist, but dramatically potentiates pH 5.5-evoked responses. f , pH dose-response of ASIC2a in the absence (dark green) or presence (light green) of 75 nM MitTx. Data were fit to the Hill equation. g , PcTx1 (100 nM) inhibits both pH 6- and MitTx-evoked currents in ASIC1a-expressing oocytes. h , MitTx occludes PcTx1 inhibition. Vertical scale bars: 1μA; horizontal bars: 1 min; V h = -60 mV.

    Journal: Nature

    Article Title: A heteromeric Texas coral snake toxin targets acid-sensing ion channels to produce pain

    doi: 10.1038/nature10607

    Figure Lengend Snippet: a , Current-voltage relationships of MitTx (300 nM)-evoked conductances from TG neurons (whole-cell configuration) demonstrate higher permeability for Na + over Cs + . The intracellular solution contained 150 mM Na + , and a leftward-shift in the reversal potential was observed when the major extracellular cation was changed from 150 mM Na + to 150 mM Cs + . b , Voltage clamp recordings show that ASIC1b-expressing oocytes respond to both extracellular acidification (H + , pH 4) and MitTx, but are insensitive to MitTxα (30 nM) or MitTxβ (300 nM) individually. Toxin-evoked responses were blocked by amiloride (Amil, 1mM). c , MitTx (75 nM)-evoked currents are comparable in magnitude to pH 4-evoked currents in ASIC1b-expressing oocytes. Toxin responses are non-desensitizing and persistent compared to transient proton-evoked currents. d , Dose-response analysis of toxin-evoked currents normalized to maximal pH 4-evoked response in ASIC-expressing oocytes. Data were fit to the Hill equation. e , MitTx (75 nM) is a poor ASIC2a agonist, but dramatically potentiates pH 5.5-evoked responses. f , pH dose-response of ASIC2a in the absence (dark green) or presence (light green) of 75 nM MitTx. Data were fit to the Hill equation. g , PcTx1 (100 nM) inhibits both pH 6- and MitTx-evoked currents in ASIC1a-expressing oocytes. h , MitTx occludes PcTx1 inhibition. Vertical scale bars: 1μA; horizontal bars: 1 min; V h = -60 mV.

    Article Snippet: PcTx1 was purified from Psalmopoeus cambridgei venom (SpiderPharm Inc.) using two sequential HPLC steps consisting of 114 minute linear gradient (0-54% acetonitrile on a semi-preparative C18 column) followed by the same gradient on an analytical C18 column (as above).

    Techniques: Permeability, Expressing, Inhibition