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  • 93
    New England Biolabs mnl i
    Assessment of <t>RNF168</t> mutation (A) and RNF168 protein level (B) . (A) Direct sequencing of RNF168 exon 12 reveals homozygosity for the novel frameshift mutation c.295delG in genomic DNA from either of both patients (HA591, HA592). (B) RFLP analysis of PCR products on 2% agarose gel electrophoresis. S, size marker; 1, undigested PCR product; 2–6, PCR products cleaved with Mnl I: 2, wild-type control, 3, paternal sample, 4. maternal sample, 5, patient HA591, 6, patient HA592. (C) Western blot analysis reveals strongly reduced immunoreactivity for RNF168 protein in lymphoblastoid cells from either of both patients (HA591, HA592). Lymphoblastoid cell lines (LCLs) from a healthy individual were used as an RNF168-proficient control (HA325), and LCLs from a patient with classical ataxia–telangiectasia were used for comparison (HA56). β-actin served as the loading control (ACTB).
    Mnl I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher mnl i
    Change of left and right HHb with methylphenidate. Effect of SNAP-25 <t>Mnl</t> I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
    Mnl I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Boehringer Mannheim mnl i
    Change of left and right HHb with methylphenidate. Effect of SNAP-25 <t>Mnl</t> I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
    Mnl I, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Stratagene mnl i
    Change of left and right HHb with methylphenidate. Effect of SNAP-25 <t>Mnl</t> I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
    Mnl I, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnl i/product/Stratagene
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    88
    Thermo Fisher fastdigest mnl i
    Change of left and right HHb with methylphenidate. Effect of SNAP-25 <t>Mnl</t> I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
    Fastdigest Mnl I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher mnl i enzyme
    Change of left and right HHb with methylphenidate. Effect of SNAP-25 <t>Mnl</t> I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
    Mnl I Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher mnl i restriction enzyme
    Change of left and right HHb with methylphenidate. Effect of SNAP-25 <t>Mnl</t> I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
    Mnl I Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore mnl i restriction enzyme
    Change of left and right HHb with methylphenidate. Effect of SNAP-25 <t>Mnl</t> I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
    Mnl I Restriction Enzyme, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega mnl i
    Change of left and right HHb with methylphenidate. Effect of SNAP-25 <t>Mnl</t> I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
    Mnl I, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Fisher Scientific mnl i
    Change of left and right HHb with methylphenidate. Effect of SNAP-25 <t>Mnl</t> I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
    Mnl I, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    GE Healthcare restriction endonuclease mnl i
    C. cayetanensis and Eimeria amplified products from nested PCR (lanes 2 to 5) and <t>RFLP</t> fragments from <t>Mnl</t> I digestion of those PCR amplified products (lanes 7 to 10). Lanes 1, 6, and 11, molecular size standards; lanes 2 and 7, negative control; lanes 3 and 8, Universidad Peruana Cayetano Heredia C. cayetanensis positive control; lanes 4 and 9, C. cayetanensis from wastewater; lanes 5 and 10, Eimeria spp. (U of A) positive control. Primers migrated to point P.
    Restriction Endonuclease Mnl I, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs mnl i endonuclease
    C. cayetanensis and Eimeria amplified products from nested PCR (lanes 2 to 5) and <t>RFLP</t> fragments from <t>Mnl</t> I digestion of those PCR amplified products (lanes 7 to 10). Lanes 1, 6, and 11, molecular size standards; lanes 2 and 7, negative control; lanes 3 and 8, Universidad Peruana Cayetano Heredia C. cayetanensis positive control; lanes 4 and 9, C. cayetanensis from wastewater; lanes 5 and 10, Eimeria spp. (U of A) positive control. Primers migrated to point P.
    Mnl I Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 7 article reviews
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    Image Search Results


    Assessment of RNF168 mutation (A) and RNF168 protein level (B) . (A) Direct sequencing of RNF168 exon 12 reveals homozygosity for the novel frameshift mutation c.295delG in genomic DNA from either of both patients (HA591, HA592). (B) RFLP analysis of PCR products on 2% agarose gel electrophoresis. S, size marker; 1, undigested PCR product; 2–6, PCR products cleaved with Mnl I: 2, wild-type control, 3, paternal sample, 4. maternal sample, 5, patient HA591, 6, patient HA592. (C) Western blot analysis reveals strongly reduced immunoreactivity for RNF168 protein in lymphoblastoid cells from either of both patients (HA591, HA592). Lymphoblastoid cell lines (LCLs) from a healthy individual were used as an RNF168-proficient control (HA325), and LCLs from a patient with classical ataxia–telangiectasia were used for comparison (HA56). β-actin served as the loading control (ACTB).

    Journal: Frontiers in Immunology

    Article Title: Clinical and Biological Manifestation of RNF168 Deficiency in Two Polish Siblings

    doi: 10.3389/fimmu.2017.01683

    Figure Lengend Snippet: Assessment of RNF168 mutation (A) and RNF168 protein level (B) . (A) Direct sequencing of RNF168 exon 12 reveals homozygosity for the novel frameshift mutation c.295delG in genomic DNA from either of both patients (HA591, HA592). (B) RFLP analysis of PCR products on 2% agarose gel electrophoresis. S, size marker; 1, undigested PCR product; 2–6, PCR products cleaved with Mnl I: 2, wild-type control, 3, paternal sample, 4. maternal sample, 5, patient HA591, 6, patient HA592. (C) Western blot analysis reveals strongly reduced immunoreactivity for RNF168 protein in lymphoblastoid cells from either of both patients (HA591, HA592). Lymphoblastoid cell lines (LCLs) from a healthy individual were used as an RNF168-proficient control (HA325), and LCLs from a patient with classical ataxia–telangiectasia were used for comparison (HA56). β-actin served as the loading control (ACTB).

    Article Snippet: For this assay, 10 µL genomic PCR products were generated with the RNF168 primer pair listed above and were incubated overnight with 1.5 U Mnl I (New England Biolabs).

    Techniques: Mutagenesis, Sequencing, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker, Western Blot

    Change of left and right HHb with methylphenidate. Effect of SNAP-25 Mnl I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.

    Journal: Journal of attention disorders

    Article Title: Association Among SNAP-25 Gene DdeI and MnlI Polymorphisms and Hemodynamic Changes During Methylphenidate Use: A Functional Near-Infrared Spectroscopy Study

    doi: 10.1177/1087054710374597

    Figure Lengend Snippet: Change of left and right HHb with methylphenidate. Effect of SNAP-25 Mnl I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.

    Article Snippet: After amplification PCR products were digested by restriction endonuclease 10 U Dde I (Fermentas, Vilnius, Lithuania) or 10 U Mnl I (Fermentas, Vilnius, Lithuania) for 14 h at 37°C.

    Techniques:

    Change of left and right HHb with methylphenidate. Effect of SNAP-25 Mnl I and Dde I polymorphisms combined. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.

    Journal: Journal of attention disorders

    Article Title: Association Among SNAP-25 Gene DdeI and MnlI Polymorphisms and Hemodynamic Changes During Methylphenidate Use: A Functional Near-Infrared Spectroscopy Study

    doi: 10.1177/1087054710374597

    Figure Lengend Snippet: Change of left and right HHb with methylphenidate. Effect of SNAP-25 Mnl I and Dde I polymorphisms combined. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.

    Article Snippet: After amplification PCR products were digested by restriction endonuclease 10 U Dde I (Fermentas, Vilnius, Lithuania) or 10 U Mnl I (Fermentas, Vilnius, Lithuania) for 14 h at 37°C.

    Techniques:

    C. cayetanensis and Eimeria amplified products from nested PCR (lanes 2 to 5) and RFLP fragments from Mnl I digestion of those PCR amplified products (lanes 7 to 10). Lanes 1, 6, and 11, molecular size standards; lanes 2 and 7, negative control; lanes 3 and 8, Universidad Peruana Cayetano Heredia C. cayetanensis positive control; lanes 4 and 9, C. cayetanensis from wastewater; lanes 5 and 10, Eimeria spp. (U of A) positive control. Primers migrated to point P.

    Journal: Applied and Environmental Microbiology

    Article Title: Detection of Cyclospora cayetanensis in Wastewater

    doi:

    Figure Lengend Snippet: C. cayetanensis and Eimeria amplified products from nested PCR (lanes 2 to 5) and RFLP fragments from Mnl I digestion of those PCR amplified products (lanes 7 to 10). Lanes 1, 6, and 11, molecular size standards; lanes 2 and 7, negative control; lanes 3 and 8, Universidad Peruana Cayetano Heredia C. cayetanensis positive control; lanes 4 and 9, C. cayetanensis from wastewater; lanes 5 and 10, Eimeria spp. (U of A) positive control. Primers migrated to point P.

    Article Snippet: Amplification products were confirmed by restriction fragment length polymorphisms (RFLP) with one unit of the restriction endonuclease Mnl I (Amersham Life Sciences Inc., Arlington Heights, Ill.) ( ).

    Techniques: Amplification, Nested PCR, Polymerase Chain Reaction, Negative Control, Positive Control