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  • 99
    Worthington Biochemical nuclease micrococcal
    Nuclease Micrococcal, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs micrococcal nuclease
    Micrococcal Nuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2706 article reviews
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    99
    Millipore micrococcal nuclease
    Bioanalyzer examination of <t>MNase</t> digested nucleosomes and protein VII-nucleosome complexes a , 195bp Nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, <t>DNA</t> extracted and analyzed. As in Fig 1g , nucleosomes are shown in black and protein VII-nucleosome complexes in orange. The presence of protein VII pauses digestion at 165bp, suggesting that protein VII is blocking access to the DNA. b , 147bp nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. Graphs show nucleosomes in grey and protein VII-nucleosome complexes in orange. The presence of protein VII completely blocks digestion even after nucleosomes alone have been digested well beyond the core particle. In contrast to what would be expected for linker histones, protein VII protects the core nucleosome particle from digestion. These data indicate that protein VII may be masking the substrate for MNase through complex formation. This represents a unique mechanism of nucleosome binding and suggests a model for blocking DNA access in cellular chromatin during infection.
    Micrococcal Nuclease, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1897 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher subcellular protein fractionation kit
    Bioanalyzer examination of <t>MNase</t> digested nucleosomes and protein VII-nucleosome complexes a , 195bp Nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, <t>DNA</t> extracted and analyzed. As in Fig 1g , nucleosomes are shown in black and protein VII-nucleosome complexes in orange. The presence of protein VII pauses digestion at 165bp, suggesting that protein VII is blocking access to the DNA. b , 147bp nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. Graphs show nucleosomes in grey and protein VII-nucleosome complexes in orange. The presence of protein VII completely blocks digestion even after nucleosomes alone have been digested well beyond the core particle. In contrast to what would be expected for linker histones, protein VII protects the core nucleosome particle from digestion. These data indicate that protein VII may be masking the substrate for MNase through complex formation. This represents a unique mechanism of nucleosome binding and suggests a model for blocking DNA access in cellular chromatin during infection.
    Subcellular Protein Fractionation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher cell lysis
    Bioanalyzer examination of <t>MNase</t> digested nucleosomes and protein VII-nucleosome complexes a , 195bp Nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, <t>DNA</t> extracted and analyzed. As in Fig 1g , nucleosomes are shown in black and protein VII-nucleosome complexes in orange. The presence of protein VII pauses digestion at 165bp, suggesting that protein VII is blocking access to the DNA. b , 147bp nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. Graphs show nucleosomes in grey and protein VII-nucleosome complexes in orange. The presence of protein VII completely blocks digestion even after nucleosomes alone have been digested well beyond the core particle. In contrast to what would be expected for linker histones, protein VII protects the core nucleosome particle from digestion. These data indicate that protein VII may be masking the substrate for MNase through complex formation. This represents a unique mechanism of nucleosome binding and suggests a model for blocking DNA access in cellular chromatin during infection.
    Cell Lysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher mnase
    Bioanalyzer examination of <t>MNase</t> digested nucleosomes and protein VII-nucleosome complexes a , 195bp Nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, <t>DNA</t> extracted and analyzed. As in Fig 1g , nucleosomes are shown in black and protein VII-nucleosome complexes in orange. The presence of protein VII pauses digestion at 165bp, suggesting that protein VII is blocking access to the DNA. b , 147bp nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. Graphs show nucleosomes in grey and protein VII-nucleosome complexes in orange. The presence of protein VII completely blocks digestion even after nucleosomes alone have been digested well beyond the core particle. In contrast to what would be expected for linker histones, protein VII protects the core nucleosome particle from digestion. These data indicate that protein VII may be masking the substrate for MNase through complex formation. This represents a unique mechanism of nucleosome binding and suggests a model for blocking DNA access in cellular chromatin during infection.
    Mnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 514 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Worthington Biochemical spleen phosphodiesterase
    Bioanalyzer examination of <t>MNase</t> digested nucleosomes and protein VII-nucleosome complexes a , 195bp Nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, <t>DNA</t> extracted and analyzed. As in Fig 1g , nucleosomes are shown in black and protein VII-nucleosome complexes in orange. The presence of protein VII pauses digestion at 165bp, suggesting that protein VII is blocking access to the DNA. b , 147bp nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. Graphs show nucleosomes in grey and protein VII-nucleosome complexes in orange. The presence of protein VII completely blocks digestion even after nucleosomes alone have been digested well beyond the core particle. In contrast to what would be expected for linker histones, protein VII protects the core nucleosome particle from digestion. These data indicate that protein VII may be masking the substrate for MNase through complex formation. This represents a unique mechanism of nucleosome binding and suggests a model for blocking DNA access in cellular chromatin during infection.
    Spleen Phosphodiesterase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher micrococcal nuclease
    Bioanalyzer examination of <t>MNase</t> digested nucleosomes and protein VII-nucleosome complexes a , 195bp Nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, <t>DNA</t> extracted and analyzed. As in Fig 1g , nucleosomes are shown in black and protein VII-nucleosome complexes in orange. The presence of protein VII pauses digestion at 165bp, suggesting that protein VII is blocking access to the DNA. b , 147bp nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. Graphs show nucleosomes in grey and protein VII-nucleosome complexes in orange. The presence of protein VII completely blocks digestion even after nucleosomes alone have been digested well beyond the core particle. In contrast to what would be expected for linker histones, protein VII protects the core nucleosome particle from digestion. These data indicate that protein VII may be masking the substrate for MNase through complex formation. This represents a unique mechanism of nucleosome binding and suggests a model for blocking DNA access in cellular chromatin during infection.
    Micrococcal Nuclease, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 999 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore rnase a
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Rnase A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 29486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher edta
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore pmsf
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Pmsf, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 32146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore benzonase
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Benzonase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    TaKaRa micrococcal nuclease
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Micrococcal Nuclease, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher tris hcl
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Tris Hcl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Worthington Biochemical calf thymus dna
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Calf Thymus Dna, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore lysis buffer
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Lysis Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 95438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tris
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Tris, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore phosphatase inhibitor cocktail
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Phosphatase Inhibitor Cocktail, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega rnasin
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 22066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Diagenode bioruptor pico
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Bioruptor Pico, supplied by Diagenode, used in various techniques. Bioz Stars score: 92/100, based on 1600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Branson Ultrasonics branson sonifier 250 d
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Branson Sonifier 250 D, supplied by Branson Ultrasonics, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore igepal ca 630
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Igepal Ca 630, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore spleen phosphodiesterase
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Spleen Phosphodiesterase, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Bioanalyzer examination of MNase digested nucleosomes and protein VII-nucleosome complexes a , 195bp Nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. As in Fig 1g , nucleosomes are shown in black and protein VII-nucleosome complexes in orange. The presence of protein VII pauses digestion at 165bp, suggesting that protein VII is blocking access to the DNA. b , 147bp nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. Graphs show nucleosomes in grey and protein VII-nucleosome complexes in orange. The presence of protein VII completely blocks digestion even after nucleosomes alone have been digested well beyond the core particle. In contrast to what would be expected for linker histones, protein VII protects the core nucleosome particle from digestion. These data indicate that protein VII may be masking the substrate for MNase through complex formation. This represents a unique mechanism of nucleosome binding and suggests a model for blocking DNA access in cellular chromatin during infection.

    Journal: Nature

    Article Title: A core viral protein binds host nucleosomes to sequester immune danger signals

    doi: 10.1038/nature18317

    Figure Lengend Snippet: Bioanalyzer examination of MNase digested nucleosomes and protein VII-nucleosome complexes a , 195bp Nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. As in Fig 1g , nucleosomes are shown in black and protein VII-nucleosome complexes in orange. The presence of protein VII pauses digestion at 165bp, suggesting that protein VII is blocking access to the DNA. b , 147bp nucleosomes or protein VII-nucleosome complexes were incubated with MNase for the indicated times, the reaction was stopped, DNA extracted and analyzed. Graphs show nucleosomes in grey and protein VII-nucleosome complexes in orange. The presence of protein VII completely blocks digestion even after nucleosomes alone have been digested well beyond the core particle. In contrast to what would be expected for linker histones, protein VII protects the core nucleosome particle from digestion. These data indicate that protein VII may be masking the substrate for MNase through complex formation. This represents a unique mechanism of nucleosome binding and suggests a model for blocking DNA access in cellular chromatin during infection.

    Article Snippet: The pelleted nuclei were resuspended in 400 µL buffer III (10 mM Tris pH 7.4, 2 mM MgCl2 , 0.1 mM PMSF) supplemented with 5mM CaCl2 and the DNA was digested to mononucleosomes by addition of 1 unit of MNase (Sigma-Aldrich, N3755).

    Techniques: Incubation, Blocking Assay, Binding Assay, Infection

    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p

    Journal: Nature

    Article Title: Induced ncRNAs Allosterically Modify RNA Binding Proteins in cis to Inhibit Transcription

    doi: 10.1038/nature06992

    Figure Lengend Snippet: Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p

    Article Snippet: RNase A, micrococcal nuclease (MNase), DNase I, RNase H and RNase T1 treatment Whole cell extracts of GST proteins were treated with RNase A (25 μg/50 μl, Sigma), and incubated on ice for 20 min. GST-TLS in whole cell extracts was sequentially treated with 10 μg of micrococcal nuclease (Roche) in 100 mM sodium glycine (pH 8.6) and 10 mM CaCl2 at 37 °C for 4 min, 0 °C for 1 min, and room temperature for 20 min, and terminated by addition of 10 mM EGTA, followed with or without incubation of 100 pmol/20 μl of RNA oligonucleotides.

    Techniques: HAT Assay, Immunoprecipitation, Activity Assay