mmp 2 Santa Cruz Biotechnology Search Results


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  • 96
    Santa Cruz Biotechnology mmp 2
    VEGF trapping up-regulates <t>MMP-2</t> expression in glioma cell lines. (A) Western blot of U87, U118, A172 and U251 glioma cells after treatment with VEGF-Ab for 5 days. Before collection, cell secretion was blocked for 6 hours with Golgi-Plug. Human β-Actin was used as a protein loading control. (B) Quantitative real-time PCR for MMP-2 mRNA levels in glioma cell lines was evaluated using the Δ C T method. Before plotted in the graph, the MMP-2 expression for each cell line was normalized to their Ig-Control treated condition, to which was given an arbitrary value of 1 (dotted, thin black line: Control). The relative expression levels of MMP-2 for all four cell lines upon VEGF-Ab treatment were then presented as fold of their Control treated cells at the indicated time points. (C) Immunocytochemistry (ICC) of glioma cell lines for MMP-2 and collagen IV after treatment with VEGF-Ab. Cells treated in vitro with VEGF-Ab for 5 days were blocked with Golgi-Plug for 6 hours before fixation for ICC. Nuclei are stained blue with DAPI; collagen IV is presented in yellow and MMP-2 in red. Bars equal 50 μ m .
    Mmp 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Santa Cruz Biotechnology anti mmp 2
    VEGF trapping up-regulates <t>MMP-2</t> expression in glioma cell lines. (A) Western blot of U87, U118, A172 and U251 glioma cells after treatment with VEGF-Ab for 5 days. Before collection, cell secretion was blocked for 6 hours with Golgi-Plug. Human β-Actin was used as a protein loading control. (B) Quantitative real-time PCR for MMP-2 mRNA levels in glioma cell lines was evaluated using the Δ C T method. Before plotted in the graph, the MMP-2 expression for each cell line was normalized to their Ig-Control treated condition, to which was given an arbitrary value of 1 (dotted, thin black line: Control). The relative expression levels of MMP-2 for all four cell lines upon VEGF-Ab treatment were then presented as fold of their Control treated cells at the indicated time points. (C) Immunocytochemistry (ICC) of glioma cell lines for MMP-2 and collagen IV after treatment with VEGF-Ab. Cells treated in vitro with VEGF-Ab for 5 days were blocked with Golgi-Plug for 6 hours before fixation for ICC. Nuclei are stained blue with DAPI; collagen IV is presented in yellow and MMP-2 in red. Bars equal 50 μ m .
    Anti Mmp 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Santa Cruz Biotechnology matrix metalloproteinase mmp 2
    VEGF trapping up-regulates <t>MMP-2</t> expression in glioma cell lines. (A) Western blot of U87, U118, A172 and U251 glioma cells after treatment with VEGF-Ab for 5 days. Before collection, cell secretion was blocked for 6 hours with Golgi-Plug. Human β-Actin was used as a protein loading control. (B) Quantitative real-time PCR for MMP-2 mRNA levels in glioma cell lines was evaluated using the Δ C T method. Before plotted in the graph, the MMP-2 expression for each cell line was normalized to their Ig-Control treated condition, to which was given an arbitrary value of 1 (dotted, thin black line: Control). The relative expression levels of MMP-2 for all four cell lines upon VEGF-Ab treatment were then presented as fold of their Control treated cells at the indicated time points. (C) Immunocytochemistry (ICC) of glioma cell lines for MMP-2 and collagen IV after treatment with VEGF-Ab. Cells treated in vitro with VEGF-Ab for 5 days were blocked with Golgi-Plug for 6 hours before fixation for ICC. Nuclei are stained blue with DAPI; collagen IV is presented in yellow and MMP-2 in red. Bars equal 50 μ m .
    Matrix Metalloproteinase Mmp 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    Santa Cruz Biotechnology reagents antibody against mmp 2
    VEGF trapping up-regulates <t>MMP-2</t> expression in glioma cell lines. (A) Western blot of U87, U118, A172 and U251 glioma cells after treatment with VEGF-Ab for 5 days. Before collection, cell secretion was blocked for 6 hours with Golgi-Plug. Human β-Actin was used as a protein loading control. (B) Quantitative real-time PCR for MMP-2 mRNA levels in glioma cell lines was evaluated using the Δ C T method. Before plotted in the graph, the MMP-2 expression for each cell line was normalized to their Ig-Control treated condition, to which was given an arbitrary value of 1 (dotted, thin black line: Control). The relative expression levels of MMP-2 for all four cell lines upon VEGF-Ab treatment were then presented as fold of their Control treated cells at the indicated time points. (C) Immunocytochemistry (ICC) of glioma cell lines for MMP-2 and collagen IV after treatment with VEGF-Ab. Cells treated in vitro with VEGF-Ab for 5 days were blocked with Golgi-Plug for 6 hours before fixation for ICC. Nuclei are stained blue with DAPI; collagen IV is presented in yellow and MMP-2 in red. Bars equal 50 μ m .
    Reagents Antibody Against Mmp 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Santa Cruz Biotechnology rabbit anti mmp 2
    VEGF trapping up-regulates <t>MMP-2</t> expression in glioma cell lines. (A) Western blot of U87, U118, A172 and U251 glioma cells after treatment with VEGF-Ab for 5 days. Before collection, cell secretion was blocked for 6 hours with Golgi-Plug. Human β-Actin was used as a protein loading control. (B) Quantitative real-time PCR for MMP-2 mRNA levels in glioma cell lines was evaluated using the Δ C T method. Before plotted in the graph, the MMP-2 expression for each cell line was normalized to their Ig-Control treated condition, to which was given an arbitrary value of 1 (dotted, thin black line: Control). The relative expression levels of MMP-2 for all four cell lines upon VEGF-Ab treatment were then presented as fold of their Control treated cells at the indicated time points. (C) Immunocytochemistry (ICC) of glioma cell lines for MMP-2 and collagen IV after treatment with VEGF-Ab. Cells treated in vitro with VEGF-Ab for 5 days were blocked with Golgi-Plug for 6 hours before fixation for ICC. Nuclei are stained blue with DAPI; collagen IV is presented in yellow and MMP-2 in red. Bars equal 50 μ m .
    Rabbit Anti Mmp 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology polyclonal rabbit anti mmp 2
    VEGF trapping up-regulates <t>MMP-2</t> expression in glioma cell lines. (A) Western blot of U87, U118, A172 and U251 glioma cells after treatment with VEGF-Ab for 5 days. Before collection, cell secretion was blocked for 6 hours with Golgi-Plug. Human β-Actin was used as a protein loading control. (B) Quantitative real-time PCR for MMP-2 mRNA levels in glioma cell lines was evaluated using the Δ C T method. Before plotted in the graph, the MMP-2 expression for each cell line was normalized to their Ig-Control treated condition, to which was given an arbitrary value of 1 (dotted, thin black line: Control). The relative expression levels of MMP-2 for all four cell lines upon VEGF-Ab treatment were then presented as fold of their Control treated cells at the indicated time points. (C) Immunocytochemistry (ICC) of glioma cell lines for MMP-2 and collagen IV after treatment with VEGF-Ab. Cells treated in vitro with VEGF-Ab for 5 days were blocked with Golgi-Plug for 6 hours before fixation for ICC. Nuclei are stained blue with DAPI; collagen IV is presented in yellow and MMP-2 in red. Bars equal 50 μ m .
    Polyclonal Rabbit Anti Mmp 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Santa Cruz Biotechnology mouse monoclonal antibody against mmp 2
    Graphical representation of mRNA expression of a <t>MMP-2</t> b MMP-9 c HOXA-11 d HOXA-10 in the endometrium of women with endometriosis and controls. e Endometrial expression of MMP-2, MMP-9, HOXA-11, HOXA-10 and β-actin in women with endometriosis
    Mouse Monoclonal Antibody Against Mmp 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 82/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Santa Cruz Biotechnology mmp2 inhibitor
    Angiogenin regulates <t>MMP2</t> expression. ( a ) Quantitative RT–PCR was performed on the test cell line panel (UROtsa-Empty, UROtsa- ANG clones 3 and 7, T24 NC siRNA, T24 ANG si-1 and si-2 and HeLa NC siRNA, HeLa ANG si-1 and si-2) to confirm ANG-induced
    Mmp2 Inhibitor, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Santa Cruz Biotechnology mmp2 goat polyclonal
    Angiogenin regulates <t>MMP2</t> expression. ( a ) Quantitative RT–PCR was performed on the test cell line panel (UROtsa-Empty, UROtsa- ANG clones 3 and 7, T24 NC siRNA, T24 ANG si-1 and si-2 and HeLa NC siRNA, HeLa ANG si-1 and si-2) to confirm ANG-induced
    Mmp2 Goat Polyclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Santa Cruz Biotechnology rabbit polyclonal antibodies against mmp2
    Angiogenin regulates <t>MMP2</t> expression. ( a ) Quantitative RT–PCR was performed on the test cell line panel (UROtsa-Empty, UROtsa- ANG clones 3 and 7, T24 NC siRNA, T24 ANG si-1 and si-2 and HeLa NC siRNA, HeLa ANG si-1 and si-2) to confirm ANG-induced
    Rabbit Polyclonal Antibodies Against Mmp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Santa Cruz Biotechnology mouse mmp2
    In vivo interaction between Mmp14a and <t>Mmp2.</t> A, B Quantitative analysis of eye size ( A ) and tectal area innervated by RGC axons ( B ) after single or combined suboptimal knockdown of Mmp14a and Mmp2 in 5 dpf embryos reveals that suboptimal knockdown of Mmp2 does not affect eye size nor OT innervation. However, combined knockdown of Mmp14a and Mmp2 results in embryos with normal eye size but a significantly reduced RGC axon innervation area in the OT, as compared to the Mmp14a suboptimal knockdown embryos ( n = 75 from 3 independent experiments). C Western blot for Mmp2 on extracts of control and Mmp14a morphant embryos at 30 hpf shows reduced levels of active Mmp2 protein (64 kDa) in Mmp14a morphant embryos. Total protein coomassie blue staining was used for loading control. Data are represented as mean ± SEM (* p
    Mouse Mmp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology mmp2 sirna
    Effect of high glucose on mitochondrial <t>MMP2</t> and its regulation in retinal endothelial cells. BRECs were transfected with <t>MMP2-siRNA</t> (si) or scrambled siRNA (SC) and were incubated in 5 mM or 20 mM glucose for 4 days. ( A ) To determine transfection efficiency, gene expression of MMP2 was quantified by semiquantitative PCR. Shown is an agarose gel image from two sets of normal (untrans) and MMP2-si–transfected (siRNA) cells. Mitochondria were prepared from the MMP2-siRNA transfected and untransfected cells incubated in normal or high glucose. ( B ) To evaluate the purity of mitochondria, protein expressions of Cox IV, H2B, and β-actin were quantified, and the Western blot is from two sets of normal untransfected cells. The activity of MMP2 was quantified in the mitochondrial fraction by ( C ) in situ zymography and ( D ) ELISA. Each measurement was made in duplicate in three to four different cell preparations, and the values obtained from the untransfected cells incubated in 5 mM glucose are considered as 100%. 5 mM, 5 mM glucose, 20 mM, 20 mM glucose; 20+si, cells transfected with MMP2-siRNA followed by 20 mM glucose treatment; 20+SC, cells transfected with scrambled RNA and treated with 20 mM glucose; mannit, 20 mM mannitol. * P
    Mmp2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Santa Cruz Biotechnology mouse monoclonal mmp2
    Immunohistochemical detection of <t>MMP2</t> staining (arrows), in liver sections in control and experimental groups (Bar: 50 μm) and semiquantitave evaluation (H-score) in liver (L) of all groups. Immunostaining intensity was assessed by semiquantation
    Mouse Monoclonal Mmp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Santa Cruz Biotechnology goat anti human mmp2 polyclonal antibody
    Immunohistochemical detection of <t>MMP2</t> staining (arrows), in liver sections in control and experimental groups (Bar: 50 μm) and semiquantitave evaluation (H-score) in liver (L) of all groups. Immunostaining intensity was assessed by semiquantation
    Goat Anti Human Mmp2 Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    VEGF trapping up-regulates MMP-2 expression in glioma cell lines. (A) Western blot of U87, U118, A172 and U251 glioma cells after treatment with VEGF-Ab for 5 days. Before collection, cell secretion was blocked for 6 hours with Golgi-Plug. Human β-Actin was used as a protein loading control. (B) Quantitative real-time PCR for MMP-2 mRNA levels in glioma cell lines was evaluated using the Δ C T method. Before plotted in the graph, the MMP-2 expression for each cell line was normalized to their Ig-Control treated condition, to which was given an arbitrary value of 1 (dotted, thin black line: Control). The relative expression levels of MMP-2 for all four cell lines upon VEGF-Ab treatment were then presented as fold of their Control treated cells at the indicated time points. (C) Immunocytochemistry (ICC) of glioma cell lines for MMP-2 and collagen IV after treatment with VEGF-Ab. Cells treated in vitro with VEGF-Ab for 5 days were blocked with Golgi-Plug for 6 hours before fixation for ICC. Nuclei are stained blue with DAPI; collagen IV is presented in yellow and MMP-2 in red. Bars equal 50 μ m .

    Journal: Gene therapy

    Article Title: Anti-angiogenic therapy increases intratumoral adenovirus distribution by inducing collagen degradation

    doi: 10.1038/gt.2012.42

    Figure Lengend Snippet: VEGF trapping up-regulates MMP-2 expression in glioma cell lines. (A) Western blot of U87, U118, A172 and U251 glioma cells after treatment with VEGF-Ab for 5 days. Before collection, cell secretion was blocked for 6 hours with Golgi-Plug. Human β-Actin was used as a protein loading control. (B) Quantitative real-time PCR for MMP-2 mRNA levels in glioma cell lines was evaluated using the Δ C T method. Before plotted in the graph, the MMP-2 expression for each cell line was normalized to their Ig-Control treated condition, to which was given an arbitrary value of 1 (dotted, thin black line: Control). The relative expression levels of MMP-2 for all four cell lines upon VEGF-Ab treatment were then presented as fold of their Control treated cells at the indicated time points. (C) Immunocytochemistry (ICC) of glioma cell lines for MMP-2 and collagen IV after treatment with VEGF-Ab. Cells treated in vitro with VEGF-Ab for 5 days were blocked with Golgi-Plug for 6 hours before fixation for ICC. Nuclei are stained blue with DAPI; collagen IV is presented in yellow and MMP-2 in red. Bars equal 50 μ m .

    Article Snippet: Antibodies and reagents The antibodies anti-human ανβ3 and anti-human ανβ5 were purchased from Chemicon/Millipore (Billerica, MA, USA); the Ig controls and anti-human CD138 from Ebioscience (San Diego, CA, USA); coxsackie-adenovirus receptor (CAR), collagen IV, adenovirus-biotin and rabbit anti-human HIF-1α from Abcam (Cambridge, MA, USA); Laminin from ABR/Thermo-Fisher Scientific (Newington, NH, USA); MMP-9 from Cell Signaling (Danvers, MA, USA) and MMP-2 from Santa Cruz (Santa Cruz, CA, USA).

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Immunocytochemistry, In Vitro, Staining

    Anti-angiogenic treatment alters the extracellular matrix (ECM) architecture of human glioma xenograft in nude mice. (A) Immunohistochemistry staining for MMP-2, Collagen IV, CD31 and Laminin. (B–E) Quantification of staining intensity was done through a computer based scoring for each of the corresponding IHC slides (n = 5 animals for each group) and mean values ± standard error of measurement (SEM) are presented in bar diagrams. Bars equal 50 μ m; *p

    Journal: Gene therapy

    Article Title: Anti-angiogenic therapy increases intratumoral adenovirus distribution by inducing collagen degradation

    doi: 10.1038/gt.2012.42

    Figure Lengend Snippet: Anti-angiogenic treatment alters the extracellular matrix (ECM) architecture of human glioma xenograft in nude mice. (A) Immunohistochemistry staining for MMP-2, Collagen IV, CD31 and Laminin. (B–E) Quantification of staining intensity was done through a computer based scoring for each of the corresponding IHC slides (n = 5 animals for each group) and mean values ± standard error of measurement (SEM) are presented in bar diagrams. Bars equal 50 μ m; *p

    Article Snippet: Antibodies and reagents The antibodies anti-human ανβ3 and anti-human ανβ5 were purchased from Chemicon/Millipore (Billerica, MA, USA); the Ig controls and anti-human CD138 from Ebioscience (San Diego, CA, USA); coxsackie-adenovirus receptor (CAR), collagen IV, adenovirus-biotin and rabbit anti-human HIF-1α from Abcam (Cambridge, MA, USA); Laminin from ABR/Thermo-Fisher Scientific (Newington, NH, USA); MMP-9 from Cell Signaling (Danvers, MA, USA) and MMP-2 from Santa Cruz (Santa Cruz, CA, USA).

    Techniques: Mouse Assay, Immunohistochemistry, Staining

    Adenovirus infection does not increase MMP-2 expression. U251 xenografts sections (A) were stained for MMP-2 with HRP and counterstained with hematoxylin; then scanned for intensity of HRP staining, presented as percent of areas with similar intensity (B). Bars represent mean intensity values of all xenografts. ns, not significant ; *** p

    Journal: Gene therapy

    Article Title: Anti-angiogenic therapy increases intratumoral adenovirus distribution by inducing collagen degradation

    doi: 10.1038/gt.2012.42

    Figure Lengend Snippet: Adenovirus infection does not increase MMP-2 expression. U251 xenografts sections (A) were stained for MMP-2 with HRP and counterstained with hematoxylin; then scanned for intensity of HRP staining, presented as percent of areas with similar intensity (B). Bars represent mean intensity values of all xenografts. ns, not significant ; *** p

    Article Snippet: Antibodies and reagents The antibodies anti-human ανβ3 and anti-human ανβ5 were purchased from Chemicon/Millipore (Billerica, MA, USA); the Ig controls and anti-human CD138 from Ebioscience (San Diego, CA, USA); coxsackie-adenovirus receptor (CAR), collagen IV, adenovirus-biotin and rabbit anti-human HIF-1α from Abcam (Cambridge, MA, USA); Laminin from ABR/Thermo-Fisher Scientific (Newington, NH, USA); MMP-9 from Cell Signaling (Danvers, MA, USA) and MMP-2 from Santa Cruz (Santa Cruz, CA, USA).

    Techniques: Infection, Expressing, Staining

    Embelin decreased vascularity and MMP2 expression in late carcinoma stage ( A ) Vascular density in colonic tumors from mice treated with or without embelin at day 85 was evaluated by immunostaining with anti-CD31 antibody. Scale bar: 50 μm. Data were quantified by blind counting of blood vessels per microscopic field ( B ). Relative mRNA expression of MMP2 in colonic tumor was analyzed by qPCR ( C ), and representative immunostaining of MMP2 in colonic tumor at day 85 after AOM/DSS exposure was shown ( D ). Scale bar: 50 μm. E. The number of MMP2 + cells per field was quantified ( n > 10 fields/group). Data are expressed as mean ± SD ( n = 6–8/group; * p

    Journal: Oncotarget

    Article Title: Macrophage targeting contributes to the inhibitory effects of embelin on colitis-associated cancer

    doi: 10.18632/oncotarget.6969

    Figure Lengend Snippet: Embelin decreased vascularity and MMP2 expression in late carcinoma stage ( A ) Vascular density in colonic tumors from mice treated with or without embelin at day 85 was evaluated by immunostaining with anti-CD31 antibody. Scale bar: 50 μm. Data were quantified by blind counting of blood vessels per microscopic field ( B ). Relative mRNA expression of MMP2 in colonic tumor was analyzed by qPCR ( C ), and representative immunostaining of MMP2 in colonic tumor at day 85 after AOM/DSS exposure was shown ( D ). Scale bar: 50 μm. E. The number of MMP2 + cells per field was quantified ( n > 10 fields/group). Data are expressed as mean ± SD ( n = 6–8/group; * p

    Article Snippet: Immunohistochemical analysis was performed as we described [ ], using the following primary antibodies: anti-CD68, anti-MR (all from Abcam, Cambridge, UK), anti-CD31 and anti-MMP2 (Santa Cruz, Dallas, USA).

    Techniques: Expressing, Mouse Assay, Immunostaining, Real-time Polymerase Chain Reaction

    Graphical representation of mRNA expression of a MMP-2 b MMP-9 c HOXA-11 d HOXA-10 in the endometrium of women with endometriosis and controls. e Endometrial expression of MMP-2, MMP-9, HOXA-11, HOXA-10 and β-actin in women with endometriosis

    Journal: Journal of Assisted Reproduction and Genetics

    Article Title: HOXA-11 mediated dysregulation of matrix remodeling during implantation window in women with endometriosis

    doi: 10.1007/s10815-013-0088-9

    Figure Lengend Snippet: Graphical representation of mRNA expression of a MMP-2 b MMP-9 c HOXA-11 d HOXA-10 in the endometrium of women with endometriosis and controls. e Endometrial expression of MMP-2, MMP-9, HOXA-11, HOXA-10 and β-actin in women with endometriosis

    Article Snippet: 30 μg of homogenate protein was separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and the separated proteins electroblotted onto a Hybond nitrocellulose membrane (GE Healthcare) at 30 V for 13 h. After blocking the non-specific binding sites with non-fat dry milk in TBST buffer for 1 h at room temperature, the blots were incubated overnight at 4 °C with mouse monoclonal antibody against MMP-2, -9 (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA) and rabbit polyclonal antibody against HOXA-10 and −11 (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA).

    Techniques: Expressing

    Angiogenin regulates MMP2 expression. ( a ) Quantitative RT–PCR was performed on the test cell line panel (UROtsa-Empty, UROtsa- ANG clones 3 and 7, T24 NC siRNA, T24 ANG si-1 and si-2 and HeLa NC siRNA, HeLa ANG si-1 and si-2) to confirm ANG-induced

    Journal: Oncogene

    Article Title: Angiogenin promotes tumoral growth and angiogenesis by regulating matrix metallopeptidase-2 expression via the ERK1/2 pathway

    doi: 10.1038/onc.2014.2

    Figure Lengend Snippet: Angiogenin regulates MMP2 expression. ( a ) Quantitative RT–PCR was performed on the test cell line panel (UROtsa-Empty, UROtsa- ANG clones 3 and 7, T24 NC siRNA, T24 ANG si-1 and si-2 and HeLa NC siRNA, HeLa ANG si-1 and si-2) to confirm ANG-induced

    Article Snippet: GSK1120212, an MAPK/ERK1/2 inhibitor (Selleck Chemicals, Houston, TX, USA), SB60015, an MAPK/JNK inhibitor (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and ARP100, an MMP2 inhibitor (Santa Cruz Biotechnology) were used in the described experiments.

    Techniques: Expressing, Quantitative RT-PCR, Clone Assay

    Myh10 deficiency leads to disrupted ECM remodeling. a Immunostaining and quantification for Fibronectin, type I collagen (COL I), and Tropoelastin on Myh10 +/+ ( n = 10) and Myh10 -/- ( n = 10) E18.5 lung sections. b Representative TEM images comparing elastic fibers in Myh10 +/+ ( n = 3) and Myh10 -/- ( n = 3) E18.5 lungs. Asterisks and arrowheads point to bundles and fragmented bundles of elastic fibers, respectively; c collagenous fibrils. c Representative western blot (from three individual sets of lung lysates) of Myh10 +/+ , Myh10 +/- , and Myh10 -/- lung lysates at E18.5. Values represent the densitometric ratio of Myh10 +/- and Myh10 -/- to Myh10 +/+ after normalization to GAPDH. d Immunostaining (from five individual sets of cultured cells) for Fibronectin in DMSO, GM6001, or MMP2 inhibitor I-treated Myh10 +/+ and Myh10 -/- embryonic fibroblasts. e Immunostaining and quantification for Fibronectin, COL I, and Tropoelastin on Myh10 -/- ( n = 5) and GM6001-injected Myh10 -/- ( n = 5) E18.5 lung sections. f Expression level of Mmp2 in EGFP siRNA and Mmp2 siRNA-treated Myh10 +/+ embryonic fibroblasts (from three replicates and individual sets of cultured cells) by RT-qPCR. g Immunostaining and quantification for Fibronectin in EGFP siRNA and Mmp2 siRNA-treated Myh10 +/+ and Myh10 -/- embryonic fibroblasts. Error bars are means ± s.e.m. ** P

    Journal: Nature Communications

    Article Title: Myh10 deficiency leads to defective extracellular matrix remodeling and pulmonary disease

    doi: 10.1038/s41467-018-06833-7

    Figure Lengend Snippet: Myh10 deficiency leads to disrupted ECM remodeling. a Immunostaining and quantification for Fibronectin, type I collagen (COL I), and Tropoelastin on Myh10 +/+ ( n = 10) and Myh10 -/- ( n = 10) E18.5 lung sections. b Representative TEM images comparing elastic fibers in Myh10 +/+ ( n = 3) and Myh10 -/- ( n = 3) E18.5 lungs. Asterisks and arrowheads point to bundles and fragmented bundles of elastic fibers, respectively; c collagenous fibrils. c Representative western blot (from three individual sets of lung lysates) of Myh10 +/+ , Myh10 +/- , and Myh10 -/- lung lysates at E18.5. Values represent the densitometric ratio of Myh10 +/- and Myh10 -/- to Myh10 +/+ after normalization to GAPDH. d Immunostaining (from five individual sets of cultured cells) for Fibronectin in DMSO, GM6001, or MMP2 inhibitor I-treated Myh10 +/+ and Myh10 -/- embryonic fibroblasts. e Immunostaining and quantification for Fibronectin, COL I, and Tropoelastin on Myh10 -/- ( n = 5) and GM6001-injected Myh10 -/- ( n = 5) E18.5 lung sections. f Expression level of Mmp2 in EGFP siRNA and Mmp2 siRNA-treated Myh10 +/+ embryonic fibroblasts (from three replicates and individual sets of cultured cells) by RT-qPCR. g Immunostaining and quantification for Fibronectin in EGFP siRNA and Mmp2 siRNA-treated Myh10 +/+ and Myh10 -/- embryonic fibroblasts. Error bars are means ± s.e.m. ** P

    Article Snippet: Cells were treated with DMSO, Blebbistatin (10 μM), GM6001 (10 μM), and MMP2 inhibitor I (Santa Cruz Biotechnology, SC-204092, 10 μM) at 37 °C in a 5% CO2 incubator for 24–48 h. Transfection was performed using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific) according to manufacturer’s instructions.

    Techniques: Immunostaining, Transmission Electron Microscopy, Western Blot, Cell Culture, Injection, Expressing, Quantitative RT-PCR

    In vivo interaction between Mmp14a and Mmp2. A, B Quantitative analysis of eye size ( A ) and tectal area innervated by RGC axons ( B ) after single or combined suboptimal knockdown of Mmp14a and Mmp2 in 5 dpf embryos reveals that suboptimal knockdown of Mmp2 does not affect eye size nor OT innervation. However, combined knockdown of Mmp14a and Mmp2 results in embryos with normal eye size but a significantly reduced RGC axon innervation area in the OT, as compared to the Mmp14a suboptimal knockdown embryos ( n = 75 from 3 independent experiments). C Western blot for Mmp2 on extracts of control and Mmp14a morphant embryos at 30 hpf shows reduced levels of active Mmp2 protein (64 kDa) in Mmp14a morphant embryos. Total protein coomassie blue staining was used for loading control. Data are represented as mean ± SEM (* p

    Journal: PLoS ONE

    Article Title: Matrix Metalloproteinase 14 in the Zebrafish: An Eye on Retinal and Retinotectal Development

    doi: 10.1371/journal.pone.0052915

    Figure Lengend Snippet: In vivo interaction between Mmp14a and Mmp2. A, B Quantitative analysis of eye size ( A ) and tectal area innervated by RGC axons ( B ) after single or combined suboptimal knockdown of Mmp14a and Mmp2 in 5 dpf embryos reveals that suboptimal knockdown of Mmp2 does not affect eye size nor OT innervation. However, combined knockdown of Mmp14a and Mmp2 results in embryos with normal eye size but a significantly reduced RGC axon innervation area in the OT, as compared to the Mmp14a suboptimal knockdown embryos ( n = 75 from 3 independent experiments). C Western blot for Mmp2 on extracts of control and Mmp14a morphant embryos at 30 hpf shows reduced levels of active Mmp2 protein (64 kDa) in Mmp14a morphant embryos. Total protein coomassie blue staining was used for loading control. Data are represented as mean ± SEM (* p

    Article Snippet: Immunostaining for Mmp14a and Mmp2 was performed using rabbit primary antibodies against zebrafish Mmp14a (Anaspec, 55115, 1/100), mouse MMP14 (Abcam, ab53712, 1/200), zebrafish Mmp2 (Anaspec, 55111, 1/100) or mouse MMP2 (Santa Cruz, sc-8835-R, 1/200), followed by a biotinylated goat anti-rabbit secondary antibody (Dako, 1/300).

    Techniques: In Vivo, Western Blot, Staining

    Effect of high glucose on mitochondrial MMP2 and its regulation in retinal endothelial cells. BRECs were transfected with MMP2-siRNA (si) or scrambled siRNA (SC) and were incubated in 5 mM or 20 mM glucose for 4 days. ( A ) To determine transfection efficiency, gene expression of MMP2 was quantified by semiquantitative PCR. Shown is an agarose gel image from two sets of normal (untrans) and MMP2-si–transfected (siRNA) cells. Mitochondria were prepared from the MMP2-siRNA transfected and untransfected cells incubated in normal or high glucose. ( B ) To evaluate the purity of mitochondria, protein expressions of Cox IV, H2B, and β-actin were quantified, and the Western blot is from two sets of normal untransfected cells. The activity of MMP2 was quantified in the mitochondrial fraction by ( C ) in situ zymography and ( D ) ELISA. Each measurement was made in duplicate in three to four different cell preparations, and the values obtained from the untransfected cells incubated in 5 mM glucose are considered as 100%. 5 mM, 5 mM glucose, 20 mM, 20 mM glucose; 20+si, cells transfected with MMP2-siRNA followed by 20 mM glucose treatment; 20+SC, cells transfected with scrambled RNA and treated with 20 mM glucose; mannit, 20 mM mannitol. * P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Novel Role of Mitochondrial Matrix Metalloproteinase-2 in the Development of Diabetic Retinopathy

    doi: 10.1167/iovs.10-6368

    Figure Lengend Snippet: Effect of high glucose on mitochondrial MMP2 and its regulation in retinal endothelial cells. BRECs were transfected with MMP2-siRNA (si) or scrambled siRNA (SC) and were incubated in 5 mM or 20 mM glucose for 4 days. ( A ) To determine transfection efficiency, gene expression of MMP2 was quantified by semiquantitative PCR. Shown is an agarose gel image from two sets of normal (untrans) and MMP2-si–transfected (siRNA) cells. Mitochondria were prepared from the MMP2-siRNA transfected and untransfected cells incubated in normal or high glucose. ( B ) To evaluate the purity of mitochondria, protein expressions of Cox IV, H2B, and β-actin were quantified, and the Western blot is from two sets of normal untransfected cells. The activity of MMP2 was quantified in the mitochondrial fraction by ( C ) in situ zymography and ( D ) ELISA. Each measurement was made in duplicate in three to four different cell preparations, and the values obtained from the untransfected cells incubated in 5 mM glucose are considered as 100%. 5 mM, 5 mM glucose, 20 mM, 20 mM glucose; 20+si, cells transfected with MMP2-siRNA followed by 20 mM glucose treatment; 20+SC, cells transfected with scrambled RNA and treated with 20 mM glucose; mannit, 20 mM mannitol. * P

    Article Snippet: Endothelial cells from passages 3 to 5 were transfected with MMP2 siRNA (Santa Cruz Biotechnology, Santa Cruz, CA) using the methods routinely used in our laboratory.

    Techniques: Transfection, Incubation, Expressing, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Western Blot, Activity Assay, In Situ, Zymography, Enzyme-linked Immunosorbent Assay

    Effect of MMP2-siRNA on mitochondrial function. Mitochondrial content of ( A ) Bax and Bcl-XL and ( B ) connexin 43 was quantified by Western blot analysis using Cox IV as a loading protein. Each measurement was performed in at least three different cell preparations. The values, represented as mean ± SD, obtained from the cells incubated in 5 mM glucose are considered as 100%. * P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Novel Role of Mitochondrial Matrix Metalloproteinase-2 in the Development of Diabetic Retinopathy

    doi: 10.1167/iovs.10-6368

    Figure Lengend Snippet: Effect of MMP2-siRNA on mitochondrial function. Mitochondrial content of ( A ) Bax and Bcl-XL and ( B ) connexin 43 was quantified by Western blot analysis using Cox IV as a loading protein. Each measurement was performed in at least three different cell preparations. The values, represented as mean ± SD, obtained from the cells incubated in 5 mM glucose are considered as 100%. * P

    Article Snippet: Endothelial cells from passages 3 to 5 were transfected with MMP2 siRNA (Santa Cruz Biotechnology, Santa Cruz, CA) using the methods routinely used in our laboratory.

    Techniques: Western Blot, Incubation

    Immunohistochemical detection of MMP2 staining (arrows), in liver sections in control and experimental groups (Bar: 50 μm) and semiquantitave evaluation (H-score) in liver (L) of all groups. Immunostaining intensity was assessed by semiquantation

    Journal: Balkan Medical Journal

    Article Title: Effects of Luteolin on Liver, Kidney and Brain in Pentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities

    doi: 10.5152/balkanmedj.2011.030

    Figure Lengend Snippet: Immunohistochemical detection of MMP2 staining (arrows), in liver sections in control and experimental groups (Bar: 50 μm) and semiquantitave evaluation (H-score) in liver (L) of all groups. Immunostaining intensity was assessed by semiquantation

    Article Snippet: The sections were incubated with rabbit polyclonal anti-eNOS (Neo Markers, dilution 1: 100), rabbit polyclonal anti-iNOS (inducible nitric oxide synthase, Neo Markers, dilution 1: 100) and mouse monoclonal MMP2 (Santa Cruz, dilution 1: 100) and goat polyclonal MMP-9 (1: 100) was applied and reacted with tissue specimens at room temperature for one hour.

    Techniques: Immunohistochemistry, Staining, Immunostaining

    Immunohistochemical detection of MMP2 staining (arrows), in kidney sections in control and experimental groups (Bar: 50 μm) and semiquantitative evaluation (H-score) in kidneys (K) of all groups. Immunostaining intensity was assessed by semiquantation

    Journal: Balkan Medical Journal

    Article Title: Effects of Luteolin on Liver, Kidney and Brain in Pentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities

    doi: 10.5152/balkanmedj.2011.030

    Figure Lengend Snippet: Immunohistochemical detection of MMP2 staining (arrows), in kidney sections in control and experimental groups (Bar: 50 μm) and semiquantitative evaluation (H-score) in kidneys (K) of all groups. Immunostaining intensity was assessed by semiquantation

    Article Snippet: The sections were incubated with rabbit polyclonal anti-eNOS (Neo Markers, dilution 1: 100), rabbit polyclonal anti-iNOS (inducible nitric oxide synthase, Neo Markers, dilution 1: 100) and mouse monoclonal MMP2 (Santa Cruz, dilution 1: 100) and goat polyclonal MMP-9 (1: 100) was applied and reacted with tissue specimens at room temperature for one hour.

    Techniques: Immunohistochemistry, Staining, Immunostaining