mmlv-rt Search Results


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  • 99
    Thermo Fisher mmlv rt
    Analysis of <t>MMLV</t> integrations in the Hoxa locus of E2a–PBX1 B-cell leukemia. ( A ) Schematic representation of MMLV integrations localized in the Hoxa locus in E2a–PBX1 transgenic mice as determined by I-PCR. Integrations are indicated by the vertical arrows above the representation of the Hoxa cluster. Large vertical bars indicate Hoxa gene exons, small vertical bars indicate the UTRs, and solid lines with arrows pointing to the left indicate the orientation of the gene and the introns. ( B ) Table showing Q-PCR results as fold difference in expression levels of Hoxa cluster genes in the Hoxa -locus-targeted tumors compared to a control non- Hoxa ), values of 8, representing three PCR cycles, and more are considered as substantial increases of expression (boxed numbers). ( C ) Semiquantitative RT–PCR analyses of Hoxa7, Hoxa9 ,and Hoxa10 expression in E2a–PBX1 B-cell leukemias that contain MMLV integration(s) in the Hoxa locus (lanes 5 – 10 ) or control B-cell leukemias (lanes 3,4 ). (S) <t>RNA</t> extracted from spleen cells of a healthy E2a–PBX1 transgenic mouse; (no RT) no reverse transcription before the PCR amplification.
    Mmlv Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1745 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1745 article reviews
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    99
    Millipore mmlv rt
    Analysis of <t>MMLV</t> integrations in the Hoxa locus of E2a–PBX1 B-cell leukemia. ( A ) Schematic representation of MMLV integrations localized in the Hoxa locus in E2a–PBX1 transgenic mice as determined by I-PCR. Integrations are indicated by the vertical arrows above the representation of the Hoxa cluster. Large vertical bars indicate Hoxa gene exons, small vertical bars indicate the UTRs, and solid lines with arrows pointing to the left indicate the orientation of the gene and the introns. ( B ) Table showing Q-PCR results as fold difference in expression levels of Hoxa cluster genes in the Hoxa -locus-targeted tumors compared to a control non- Hoxa ), values of 8, representing three PCR cycles, and more are considered as substantial increases of expression (boxed numbers). ( C ) Semiquantitative RT–PCR analyses of Hoxa7, Hoxa9 ,and Hoxa10 expression in E2a–PBX1 B-cell leukemias that contain MMLV integration(s) in the Hoxa locus (lanes 5 – 10 ) or control B-cell leukemias (lanes 3,4 ). (S) <t>RNA</t> extracted from spleen cells of a healthy E2a–PBX1 transgenic mouse; (no RT) no reverse transcription before the PCR amplification.
    Mmlv Rt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare mmlv rt
    Analysis of <t>MMLV</t> integrations in the Hoxa locus of E2a–PBX1 B-cell leukemia. ( A ) Schematic representation of MMLV integrations localized in the Hoxa locus in E2a–PBX1 transgenic mice as determined by I-PCR. Integrations are indicated by the vertical arrows above the representation of the Hoxa cluster. Large vertical bars indicate Hoxa gene exons, small vertical bars indicate the UTRs, and solid lines with arrows pointing to the left indicate the orientation of the gene and the introns. ( B ) Table showing Q-PCR results as fold difference in expression levels of Hoxa cluster genes in the Hoxa -locus-targeted tumors compared to a control non- Hoxa ), values of 8, representing three PCR cycles, and more are considered as substantial increases of expression (boxed numbers). ( C ) Semiquantitative RT–PCR analyses of Hoxa7, Hoxa9 ,and Hoxa10 expression in E2a–PBX1 B-cell leukemias that contain MMLV integration(s) in the Hoxa locus (lanes 5 – 10 ) or control B-cell leukemias (lanes 3,4 ). (S) <t>RNA</t> extracted from spleen cells of a healthy E2a–PBX1 transgenic mouse; (no RT) no reverse transcription before the PCR amplification.
    Mmlv Rt, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega mmlv rt
    Exogenous reverse transcription reactions with MN-treated pFOXC RT and <t>MMLV</t> RT. Reactions with mtRNP particles isolated from pFOXC3-containing strains digested with MN (lanes 1 to 5) or MMLV RT (lanes 7 to 10). Lane 1, no exogenous RNA. Lanes 2 to 5 and 7 to 10, reaction mixtures containing 93-nt C3-2R RNA that corresponds to the 3′ terminus of the pFOXC3 plasmid transcript. Reactions were carried out with (lanes 4, 5, 9, and 10) or without (lanes 2, 3, 7, and 8) a 34-nt oligonucleotide that is complementary to a 25-nt region at the 3′ end of the RNA template. Following <t>cDNA</t> synthesis, products were incubated with RNase A (lanes 3, 5, 8, and 10) or left untreated (lanes 1, 2, 4, 7, and 9), prior to electrophoresis in a 6% polyacrylamide gel containing 8 M urea. Numbers on the left indicate the sizes (nucleotides) of a 100-bp marker and Sau3AI fragments of pBS(−) molecular weight markers (M, lane 6). Numbers on the right indicate the sizes (in nucleotides) of the 32 P-labeled DNA products as well as a schematic drawing of the most favorable base-pairing interactions of snapped-back C3-2R RNAs that may correspond to the products observed (dashed line, C3-2R RNA; solid line, cDNA; open box, 2R oligonucleotide). Potential base-pairing interactions of the 2R oligonucleotide self-dimer that is extended by the pFOXC RT are shown at the bottom, with the incorporated nucleotides indicated in lowercase letters.
    Mmlv Rt, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche mmlv rt
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    iNtRON Biotechnology mmlv rt
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene mmlv rt
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Prospec mmlv rt
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt, supplied by Prospec, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mmlv rt
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega mmlv rt buffer
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega mmlv rt enzyme
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Evrogen mmlv rt kit
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt Kit, supplied by Evrogen, used in various techniques. Bioz Stars score: 92/100, based on 376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Toyobo variant mmlv rt
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Variant Mmlv Rt, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    iNtRON Biotechnology mmlv rt buffer
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt Buffer, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher mmlv rt kit
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    GE Healthcare mmlv rt buffer
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa mmlv rt buffer
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primescript mmlv rt
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Primescript Mmlv Rt, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher wt mmlv rt
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Wt Mmlv Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega mmlv rt kit
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega mmlv rt system
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa mmlv rt kit
    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For <t>QRT-PCR</t> analysis (D), total RNA (1 µg) was reverse-transcribed using <t>MMLV</t> RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P
    Mmlv Rt Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Analysis of MMLV integrations in the Hoxa locus of E2a–PBX1 B-cell leukemia. ( A ) Schematic representation of MMLV integrations localized in the Hoxa locus in E2a–PBX1 transgenic mice as determined by I-PCR. Integrations are indicated by the vertical arrows above the representation of the Hoxa cluster. Large vertical bars indicate Hoxa gene exons, small vertical bars indicate the UTRs, and solid lines with arrows pointing to the left indicate the orientation of the gene and the introns. ( B ) Table showing Q-PCR results as fold difference in expression levels of Hoxa cluster genes in the Hoxa -locus-targeted tumors compared to a control non- Hoxa ), values of 8, representing three PCR cycles, and more are considered as substantial increases of expression (boxed numbers). ( C ) Semiquantitative RT–PCR analyses of Hoxa7, Hoxa9 ,and Hoxa10 expression in E2a–PBX1 B-cell leukemias that contain MMLV integration(s) in the Hoxa locus (lanes 5 – 10 ) or control B-cell leukemias (lanes 3,4 ). (S) RNA extracted from spleen cells of a healthy E2a–PBX1 transgenic mouse; (no RT) no reverse transcription before the PCR amplification.

    Journal: Genes & Development

    Article Title: High incidence of proviral integrations in the Hoxa locus in a new model of E2a-PBX1-induced B-cell leukemia

    doi: 10.1101/gad.1268505

    Figure Lengend Snippet: Analysis of MMLV integrations in the Hoxa locus of E2a–PBX1 B-cell leukemia. ( A ) Schematic representation of MMLV integrations localized in the Hoxa locus in E2a–PBX1 transgenic mice as determined by I-PCR. Integrations are indicated by the vertical arrows above the representation of the Hoxa cluster. Large vertical bars indicate Hoxa gene exons, small vertical bars indicate the UTRs, and solid lines with arrows pointing to the left indicate the orientation of the gene and the introns. ( B ) Table showing Q-PCR results as fold difference in expression levels of Hoxa cluster genes in the Hoxa -locus-targeted tumors compared to a control non- Hoxa ), values of 8, representing three PCR cycles, and more are considered as substantial increases of expression (boxed numbers). ( C ) Semiquantitative RT–PCR analyses of Hoxa7, Hoxa9 ,and Hoxa10 expression in E2a–PBX1 B-cell leukemias that contain MMLV integration(s) in the Hoxa locus (lanes 5 – 10 ) or control B-cell leukemias (lanes 3,4 ). (S) RNA extracted from spleen cells of a healthy E2a–PBX1 transgenic mouse; (no RT) no reverse transcription before the PCR amplification.

    Article Snippet: Total RNA was isolated by Trizol, DNase-I-treated, and cDNA was prepared (MMLV-RT, random primers) according to the manufacturer's instructions (InVitrogen).

    Techniques: Transgenic Assay, Mouse Assay, Polymerase Chain Reaction, Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification

    Exogenous reverse transcription reactions with MN-treated pFOXC RT and MMLV RT. Reactions with mtRNP particles isolated from pFOXC3-containing strains digested with MN (lanes 1 to 5) or MMLV RT (lanes 7 to 10). Lane 1, no exogenous RNA. Lanes 2 to 5 and 7 to 10, reaction mixtures containing 93-nt C3-2R RNA that corresponds to the 3′ terminus of the pFOXC3 plasmid transcript. Reactions were carried out with (lanes 4, 5, 9, and 10) or without (lanes 2, 3, 7, and 8) a 34-nt oligonucleotide that is complementary to a 25-nt region at the 3′ end of the RNA template. Following cDNA synthesis, products were incubated with RNase A (lanes 3, 5, 8, and 10) or left untreated (lanes 1, 2, 4, 7, and 9), prior to electrophoresis in a 6% polyacrylamide gel containing 8 M urea. Numbers on the left indicate the sizes (nucleotides) of a 100-bp marker and Sau3AI fragments of pBS(−) molecular weight markers (M, lane 6). Numbers on the right indicate the sizes (in nucleotides) of the 32 P-labeled DNA products as well as a schematic drawing of the most favorable base-pairing interactions of snapped-back C3-2R RNAs that may correspond to the products observed (dashed line, C3-2R RNA; solid line, cDNA; open box, 2R oligonucleotide). Potential base-pairing interactions of the 2R oligonucleotide self-dimer that is extended by the pFOXC RT are shown at the bottom, with the incorporated nucleotides indicated in lowercase letters.

    Journal: Eukaryotic Cell

    Article Title: Relaxed Primer Specificity Associated with Reverse Transcriptases Encoded by the pFOXC Retroplasmids of Fusarium oxysporum

    doi: 10.1128/EC.3.6.1589-1600.2004

    Figure Lengend Snippet: Exogenous reverse transcription reactions with MN-treated pFOXC RT and MMLV RT. Reactions with mtRNP particles isolated from pFOXC3-containing strains digested with MN (lanes 1 to 5) or MMLV RT (lanes 7 to 10). Lane 1, no exogenous RNA. Lanes 2 to 5 and 7 to 10, reaction mixtures containing 93-nt C3-2R RNA that corresponds to the 3′ terminus of the pFOXC3 plasmid transcript. Reactions were carried out with (lanes 4, 5, 9, and 10) or without (lanes 2, 3, 7, and 8) a 34-nt oligonucleotide that is complementary to a 25-nt region at the 3′ end of the RNA template. Following cDNA synthesis, products were incubated with RNase A (lanes 3, 5, 8, and 10) or left untreated (lanes 1, 2, 4, 7, and 9), prior to electrophoresis in a 6% polyacrylamide gel containing 8 M urea. Numbers on the left indicate the sizes (nucleotides) of a 100-bp marker and Sau3AI fragments of pBS(−) molecular weight markers (M, lane 6). Numbers on the right indicate the sizes (in nucleotides) of the 32 P-labeled DNA products as well as a schematic drawing of the most favorable base-pairing interactions of snapped-back C3-2R RNAs that may correspond to the products observed (dashed line, C3-2R RNA; solid line, cDNA; open box, 2R oligonucleotide). Potential base-pairing interactions of the 2R oligonucleotide self-dimer that is extended by the pFOXC RT are shown at the bottom, with the incorporated nucleotides indicated in lowercase letters.

    Article Snippet: Interestingly, the major cDNA products obtained with MMLV RT were significantly shorter (73, 76, and 82 nt) than those obtained with the MN-treated pFOXC RT.

    Techniques: Isolation, Plasmid Preparation, Incubation, Electrophoresis, Marker, Molecular Weight, Labeling

    INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For QRT-PCR analysis (D), total RNA (1 µg) was reverse-transcribed using MMLV RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P

    Journal: PLoS ONE

    Article Title: Invading Basement Membrane Matrix Is Sufficient for MDA-MB-231 Breast Cancer Cells to Develop a Stable In Vivo Metastatic Phenotype

    doi: 10.1371/journal.pone.0023334

    Figure Lengend Snippet: INV and REF breast cancer cell invasion and migration. Cells (2.5×10 5 ) were added to the upper side of each 8 µm insert of Boyden chambers coated (A) or not (B) with Matrigel for invasion or migration assays respectively; cells were then counted as described in Materials and Methods . MMP-9 activity secreted by REF (lane 1) and INV (lane 2) cells (C) was assessed by subjecting aliquots of lyophilized conditioned media normalized to the number of cells to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. For QRT-PCR analysis (D), total RNA (1 µg) was reverse-transcribed using MMLV RT and subjected to qRT-PCR as described in Material and Methods . Representative data was normalised to PPIA is given. Each column represents a mean (± SD) of three independent experiments. * P

    Article Snippet: Real-time RT-PCR analysis Total RNA (1 µg) was reverse-transcribed using MMLV RT (Invitogen, Carlsbad, CA, USA) and random primers from Roche Applied Science (Roche, Indianapolis, IN, USA).

    Techniques: Migration, Activity Assay, Quantitative RT-PCR