mme i New England Biolabs Search Results


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  • 93
    New England Biolabs mme i
    Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the <t>Mme</t> I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.
    Mme I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs mme i enzyme
    Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the <t>Mme</t> I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.
    Mme I Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs mme i digestion
    Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the <t>Mme</t> I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.
    Mme I Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    New England Biolabs restriction enzyme mme i
    Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the <t>Mme</t> I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.
    Restriction Enzyme Mme I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs mme i restriction enzymes
    Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the <t>Mme</t> I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.
    Mme I Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    New England Biolabs type iis restriction enzyme mme i
    Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the <t>Mme</t> I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.
    Type Iis Restriction Enzyme Mme I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs type iii endonuclease mme i
    Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the <t>Mme</t> I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.
    Type Iii Endonuclease Mme I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs type iis restriction enzyme
    Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the <t>Mme</t> I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.
    Type Iis Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    New England Biolabs nla iii
    Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with <t>Nla</t> <t>III.</t> All subsequent steps in the protocol are identical.
    Nla Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs high fidelity polymerase
    Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with <t>Nla</t> <t>III.</t> All subsequent steps in the protocol are identical.
    High Fidelity Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs s adenosylmethionine
    Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with <t>Nla</t> <t>III.</t> All subsequent steps in the protocol are identical.
    S Adenosylmethionine, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs oligo d
    Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with <t>Nla</t> <t>III.</t> All subsequent steps in the protocol are identical.
    Oligo D, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs antarctic phosphatase
    Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with <t>Nla</t> <t>III.</t> All subsequent steps in the protocol are identical.
    Antarctic Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4986 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs neb buffer 4
    Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with <t>Nla</t> <t>III.</t> All subsequent steps in the protocol are identical.
    Neb Buffer 4, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bst dna polymerase
    Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with <t>Nla</t> <t>III.</t> All subsequent steps in the protocol are identical.
    Bst Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs phusion taq
    Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with <t>Nla</t> <t>III.</t> All subsequent steps in the protocol are identical.
    Phusion Taq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    New England Biolabs bsg i
    ChlaMmeSeq Is an <t>Mme</t> I-Based Strategy for Mapping Insertion Sites. (A) The cassette used to transform C. reinhardtii cells is 2660 bp long and is composed of a PSAD promoter, AphVIII gene (conferring paromomycin resistance), CYTc6 intron, and PSAD and RPL12 terminators in opposite orientations. Restriction enzyme sites ( Mme I and Bsg I) are shown. Blue lines represent genomic DNA. (B) Double digestion of mutant genomic DNA with Mme I and <t>Bsg</t> I yields a 1121-bp fragment from each side of the cassette, containing 20 to 21 bp of flanking genomic DNA with a two-nucleotide overhang. (C) An adaptor (orange) that contains both PCR and sequencing primer binding sites is ligated to the digestion products. (D) The flanking DNA sequences are amplified with PCR primers (black and orange arrows) binding to the cassette and adaptors. (E) The resulting PCR product is sequenced by Sanger or Illumina sequencing (for individual or pooled mutants, respectively). (F) The 20- to 21-bp flanking DNA sequences are mapped to the C. reinhardtii genome to identify the site of each insertion.
    Bsg I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t4 dna ligase
    ChlaMmeSeq Is an <t>Mme</t> I-Based Strategy for Mapping Insertion Sites. (A) The cassette used to transform C. reinhardtii cells is 2660 bp long and is composed of a PSAD promoter, AphVIII gene (conferring paromomycin resistance), CYTc6 intron, and PSAD and RPL12 terminators in opposite orientations. Restriction enzyme sites ( Mme I and Bsg I) are shown. Blue lines represent genomic DNA. (B) Double digestion of mutant genomic DNA with Mme I and <t>Bsg</t> I yields a 1121-bp fragment from each side of the cassette, containing 20 to 21 bp of flanking genomic DNA with a two-nucleotide overhang. (C) An adaptor (orange) that contains both PCR and sequencing primer binding sites is ligated to the digestion products. (D) The flanking DNA sequences are amplified with PCR primers (black and orange arrows) binding to the cassette and adaptors. (E) The resulting PCR product is sequenced by Sanger or Illumina sequencing (for individual or pooled mutants, respectively). (F) The 20- to 21-bp flanking DNA sequences are mapped to the C. reinhardtii genome to identify the site of each insertion.
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 38631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs rnase treatment
    ChlaMmeSeq Is an <t>Mme</t> I-Based Strategy for Mapping Insertion Sites. (A) The cassette used to transform C. reinhardtii cells is 2660 bp long and is composed of a PSAD promoter, AphVIII gene (conferring paromomycin resistance), CYTc6 intron, and PSAD and RPL12 terminators in opposite orientations. Restriction enzyme sites ( Mme I and Bsg I) are shown. Blue lines represent genomic DNA. (B) Double digestion of mutant genomic DNA with Mme I and <t>Bsg</t> I yields a 1121-bp fragment from each side of the cassette, containing 20 to 21 bp of flanking genomic DNA with a two-nucleotide overhang. (C) An adaptor (orange) that contains both PCR and sequencing primer binding sites is ligated to the digestion products. (D) The flanking DNA sequences are amplified with PCR primers (black and orange arrows) binding to the cassette and adaptors. (E) The resulting PCR product is sequenced by Sanger or Illumina sequencing (for individual or pooled mutants, respectively). (F) The 20- to 21-bp flanking DNA sequences are mapped to the C. reinhardtii genome to identify the site of each insertion.
    Rnase Treatment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    New England Biolabs restriction enzyme hpa ii
    ChlaMmeSeq Is an <t>Mme</t> I-Based Strategy for Mapping Insertion Sites. (A) The cassette used to transform C. reinhardtii cells is 2660 bp long and is composed of a PSAD promoter, AphVIII gene (conferring paromomycin resistance), CYTc6 intron, and PSAD and RPL12 terminators in opposite orientations. Restriction enzyme sites ( Mme I and Bsg I) are shown. Blue lines represent genomic DNA. (B) Double digestion of mutant genomic DNA with Mme I and <t>Bsg</t> I yields a 1121-bp fragment from each side of the cassette, containing 20 to 21 bp of flanking genomic DNA with a two-nucleotide overhang. (C) An adaptor (orange) that contains both PCR and sequencing primer binding sites is ligated to the digestion products. (D) The flanking DNA sequences are amplified with PCR primers (black and orange arrows) binding to the cassette and adaptors. (E) The resulting PCR product is sequenced by Sanger or Illumina sequencing (for individual or pooled mutants, respectively). (F) The 20- to 21-bp flanking DNA sequences are mapped to the C. reinhardtii genome to identify the site of each insertion.
    Restriction Enzyme Hpa Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the Mme I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.

    Journal: BMC Genomics

    Article Title: Distributed probing of chromatin structure in vivo reveals pervasive chromatin accessibility for expressed and non-expressed genes during tissue differentiation in C. elegans

    doi: 10.1186/1471-2164-11-465

    Figure Lengend Snippet: Schematic of DALEC . Brown and green represent priming regions for Solexa bridge amplification primers. Blue indicates the primer binding region for the Solexa sequencing reaction. TCCGAC is the Mme I recognition sequence and the sequence immediately upstream of it (black) is the variable region. The inset gives detailed information for linkers A and B.

    Article Snippet: Ligation to Linker B Linker B was purchased as two separate oligonucleotides (5'P-AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGTGGTCGCCGTATCATT-OH3', 5'OH-TCATCTTTCCCTACACGACGCTCTTCCGATCTNN-OH3') and hybridized using the same procedure as described for Linker A. Mme I products were ligated to Linker B using the following 50 μl reaction mix: 10.0 μl Mme I product, 1.0 μl of 0.05 mM Linker B, 5.0 μl 10× ligase buffer, 3.0 μl T4 DNA ligase (2,000 U/μl; NEB #M0202), 31.0 μl dH2 O. Ligations were performed overnight using a PCR machine.

    Techniques: Amplification, Binding Assay, Sequencing

    Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with Nla III. All subsequent steps in the protocol are identical.

    Journal: Genome Research

    Article Title: Genomic Signature Tags (GSTs): A System for Profiling Genomic DNA

    doi: 10.1101/gr.306102

    Figure Lengend Snippet: Schematic for GST preparation. In this method, DNA is first fragmented with a rare cutter such as Not I or a more frequent cutter such as Bam HI. Specific complementary biotinylated linkers are ligated to the free ends, and the DNA is then digested with Nla III. All subsequent steps in the protocol are identical.

    Article Snippet: A second incubation with Nla III was performed on the bound fragments by resuspending the beads in 200 μL Nla III digestion buffer containing 25 U of enzyme and incubating for 2 h at 37°C, after which an additional 25 U of enzyme was added and incubation continued for 2 h. The beads were washed three times with 200 μL TEsl, to remove nonbound DNA fragments, and one time with 200 μL 1× T4 ligase buffer.

    Techniques:

    ChlaMmeSeq Is an Mme I-Based Strategy for Mapping Insertion Sites. (A) The cassette used to transform C. reinhardtii cells is 2660 bp long and is composed of a PSAD promoter, AphVIII gene (conferring paromomycin resistance), CYTc6 intron, and PSAD and RPL12 terminators in opposite orientations. Restriction enzyme sites ( Mme I and Bsg I) are shown. Blue lines represent genomic DNA. (B) Double digestion of mutant genomic DNA with Mme I and Bsg I yields a 1121-bp fragment from each side of the cassette, containing 20 to 21 bp of flanking genomic DNA with a two-nucleotide overhang. (C) An adaptor (orange) that contains both PCR and sequencing primer binding sites is ligated to the digestion products. (D) The flanking DNA sequences are amplified with PCR primers (black and orange arrows) binding to the cassette and adaptors. (E) The resulting PCR product is sequenced by Sanger or Illumina sequencing (for individual or pooled mutants, respectively). (F) The 20- to 21-bp flanking DNA sequences are mapped to the C. reinhardtii genome to identify the site of each insertion.

    Journal: The Plant Cell

    Article Title: High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA [W]High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA [W] [OPEN]

    doi: 10.1105/tpc.114.124099

    Figure Lengend Snippet: ChlaMmeSeq Is an Mme I-Based Strategy for Mapping Insertion Sites. (A) The cassette used to transform C. reinhardtii cells is 2660 bp long and is composed of a PSAD promoter, AphVIII gene (conferring paromomycin resistance), CYTc6 intron, and PSAD and RPL12 terminators in opposite orientations. Restriction enzyme sites ( Mme I and Bsg I) are shown. Blue lines represent genomic DNA. (B) Double digestion of mutant genomic DNA with Mme I and Bsg I yields a 1121-bp fragment from each side of the cassette, containing 20 to 21 bp of flanking genomic DNA with a two-nucleotide overhang. (C) An adaptor (orange) that contains both PCR and sequencing primer binding sites is ligated to the digestion products. (D) The flanking DNA sequences are amplified with PCR primers (black and orange arrows) binding to the cassette and adaptors. (E) The resulting PCR product is sequenced by Sanger or Illumina sequencing (for individual or pooled mutants, respectively). (F) The 20- to 21-bp flanking DNA sequences are mapped to the C. reinhardtii genome to identify the site of each insertion.

    Article Snippet: The 500-μL reactions were assembled with 3.5 μg DNA, 50 μL 10× NEB4 buffer, 1.25 μL 32 mM S -adenosyl methionine, 5 μL 10 mg/mL BSA, 40 μL 2 units/μL Mme I, and 1.25 μL 5 units/μL Bsg I (NEB).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Sequencing, Binding Assay, Amplification