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    New England Biolabs mlui
    Digestion patterns of type A (ATCC 3502) and type B (FT 243) proteolytic C. botulinum strains using the rare-cutting restriction enzymes <t>ApaI,</t> AscI, <t>MluI,</t> NruI, PmeI, and RsrII. The pulse time ramp was 1 to 22 s, and the running time was 20 h. The outermost
    Mlui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    New England Biolabs mlui hf
    Digestion patterns of type A (ATCC 3502) and type B (FT 243) proteolytic C. botulinum strains using the rare-cutting restriction enzymes <t>ApaI,</t> AscI, <t>MluI,</t> NruI, PmeI, and RsrII. The pulse time ramp was 1 to 22 s, and the running time was 20 h. The outermost
    Mlui Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs mlui aatii
    Schematic representation of MV-GFP and human λ light immunoglobulin chain displaying MV-λ genomes. ( a ) Human λ chain gene was inserted by exchanging the GFP using a <t>MluI/AatII</t> cleavage site upstream of the N protein. Growth kinetics
    Mlui Aatii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs csf1r rtta
    Schematic representation of MV-GFP and human λ light immunoglobulin chain displaying MV-λ genomes. ( a ) Human λ chain gene was inserted by exchanging the GFP using a <t>MluI/AatII</t> cleavage site upstream of the N protein. Growth kinetics
    Csf1r Rtta, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs mlui ndei restriction enzymes
    Schematic representation of MV-GFP and human λ light immunoglobulin chain displaying MV-λ genomes. ( a ) Human λ chain gene was inserted by exchanging the GFP using a <t>MluI/AatII</t> cleavage site upstream of the N protein. Growth kinetics
    Mlui Ndei Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bamhi hf
    Schematic representation of MV-GFP and human λ light immunoglobulin chain displaying MV-λ genomes. ( a ) Human λ chain gene was inserted by exchanging the GFP using a <t>MluI/AatII</t> cleavage site upstream of the N protein. Growth kinetics
    Bamhi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Digestion patterns of type A (ATCC 3502) and type B (FT 243) proteolytic C. botulinum strains using the rare-cutting restriction enzymes ApaI, AscI, MluI, NruI, PmeI, and RsrII. The pulse time ramp was 1 to 22 s, and the running time was 20 h. The outermost

    Journal:

    Article Title: Diversity of Proteolytic Clostridium botulinum Strains, Determined by a Pulsed-Field Gel Electrophoresis Approach

    doi: 10.1128/AEM.71.3.1311-1317.2005

    Figure Lengend Snippet: Digestion patterns of type A (ATCC 3502) and type B (FT 243) proteolytic C. botulinum strains using the rare-cutting restriction enzymes ApaI, AscI, MluI, NruI, PmeI, and RsrII. The pulse time ramp was 1 to 22 s, and the running time was 20 h. The outermost

    Article Snippet: Nine rare-cutting restriction enzymes, ApaI, AscI, MluI, NruI, PmeI, RsrII, SacII, SmaI, and XhoI (New England Biolabs), were chosen for testing the cleavage of DNA of proteolytic C. botulinum .

    Techniques:

    Generation and identification of multi-transgenic PFFs and minipigs. (A) Schematic structure of tissue-specific polycistronic system (8,840 bp). The head-to-head arrows represent the primers for transgenic recognition, copy number measurement and gene expression analysis. The fragment between the two restriction sites comprises two cassettes isolated by an insulator: ( 1 ) 11β-HSD1 driven by the liver-specific PapoE; ( 2 ) hIAPP and CHOP linked to the F-2A peptide driven by the PIP. (B) PCR screening of multi-transgenic PFFs. Amplification of the PapoE-11b, PIP-CHOP, CHOP-IAPP and IAPP-pA fragments is shown, respectively. Lanes 42–44, three representative PFFs transfected by the vector. (C) Multi-transgenic piglets produced by somatic cell nuclear transfer. (D) Genomic DNA PCR identification of piglet 1 # , 2 # and negative control. F1–F3 indicate the three anticipated bands corresponding to PapoE-11b, PIP-CHOP and CHOP-IAPP, respectively. Tg, transgenic; 11β-HSD1, 11-β-hydroxysteroid dehydrogenase 1; PapoE; hIAPP; human islet amyloid polypeptide; PIP, porcine pancreas-specific insulin promoter; CHOP; C/EBP homologous protein; PCR, polymerase chain reaction; V, positive vector; N, negative control; M, 100 bp DNA ladder; W, ddH2O. MluI, Mlu I restriction enzyme site; NotI, Not I restriction enzyme site; PFFs, porcine fetal fibroblasts.

    Journal: Molecular Medicine Reports

    Article Title: Multi-transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis

    doi: 10.3892/mmr.2015.4582

    Figure Lengend Snippet: Generation and identification of multi-transgenic PFFs and minipigs. (A) Schematic structure of tissue-specific polycistronic system (8,840 bp). The head-to-head arrows represent the primers for transgenic recognition, copy number measurement and gene expression analysis. The fragment between the two restriction sites comprises two cassettes isolated by an insulator: ( 1 ) 11β-HSD1 driven by the liver-specific PapoE; ( 2 ) hIAPP and CHOP linked to the F-2A peptide driven by the PIP. (B) PCR screening of multi-transgenic PFFs. Amplification of the PapoE-11b, PIP-CHOP, CHOP-IAPP and IAPP-pA fragments is shown, respectively. Lanes 42–44, three representative PFFs transfected by the vector. (C) Multi-transgenic piglets produced by somatic cell nuclear transfer. (D) Genomic DNA PCR identification of piglet 1 # , 2 # and negative control. F1–F3 indicate the three anticipated bands corresponding to PapoE-11b, PIP-CHOP and CHOP-IAPP, respectively. Tg, transgenic; 11β-HSD1, 11-β-hydroxysteroid dehydrogenase 1; PapoE; hIAPP; human islet amyloid polypeptide; PIP, porcine pancreas-specific insulin promoter; CHOP; C/EBP homologous protein; PCR, polymerase chain reaction; V, positive vector; N, negative control; M, 100 bp DNA ladder; W, ddH2O. MluI, Mlu I restriction enzyme site; NotI, Not I restriction enzyme site; PFFs, porcine fetal fibroblasts.

    Article Snippet: The multiple cloning site of pcDNA3.1 (+) was digested using the Mlu I and Not I enzymes (New England Biolabs, Beijing, China), and the cytomegalovirus (CMV) promoter fragment was replaced with this synthesized fragment.

    Techniques: Transgenic Assay, Expressing, Isolation, Polymerase Chain Reaction, Amplification, Transfection, Plasmid Preparation, Produced, Negative Control

    Construction and characterization of MV-expressing hGCSF . ( a ) Schematic showing cloning of DNA encoding human cytokine granulocyte colony-stimulating factor (hGCSF) p (+) MV-NSe upstream of M, using AatII and MluI restriction enzymes. ( b ) One step growth

    Journal: Molecular Therapy

    Article Title: The Role of Neutrophils in Measles Virus–mediated Oncolysis Differs Between B-cell Malignancies and Is Not Always Enhanced by GCSF

    doi: 10.1038/mt.2015.149

    Figure Lengend Snippet: Construction and characterization of MV-expressing hGCSF . ( a ) Schematic showing cloning of DNA encoding human cytokine granulocyte colony-stimulating factor (hGCSF) p (+) MV-NSe upstream of M, using AatII and MluI restriction enzymes. ( b ) One step growth

    Article Snippet: It was then PCR amplified with the following primers, forward primer MluI+hGCSF: 5′- agtattac acgcgt atggctggacctgccacccagagc-3′ and reverse primer: AatII_hGCSF: 5′-tacagtcg gacgtc attcagggctgggcaaggtggcg-3′, and the PCR product was cloned into the MVNSe backbone using AatII and MluI restriction endonucleases (New England Biolab, UK), replacing GFP, upstream of MV M gene.

    Techniques: Expressing, Clone Assay

    Validation of the URMAC method by insertion (I), substitution (S), or deletion (D) of some restriction sites in pUC18 plasmid. (A) Illustration of the Modification Target (NdeI restriction site) relative to the flanking restriction sites and location of the Starter Primers SP1 and SP2. After the first PCR, the Starter DNA migrated as expected, 532 bp on a 1% agarose gel (photo, arrow at right). A 100 bp DNA size ladder is shown in left lane for comparison. (B) Diagram of the strategy for I, S, or D using the Closed Starter DNA circularized from the PCR product in (A) as template and the Opener/Mutagenic Primers . The top photo shows the PCR product, Intermediate DNA , which contained the mutations. The bottom photo shows the Modified DNA after enrichment PCR step using SP1 and SP2. (C) Validation of URMAC mutagenesis for the three different types of mutations by restriction analysis. Fig 2C shows bands of expected DNA fragment size after digestion with respective restriction enzymes. In the control Starter PCR lane, only DNA treated with NdeI enzyme, cut the DNA into two fragments of 382 150 bp. Untreated DNA or DNA treated with MluI remained at the full size of 532 bp. In the Insertion lane, both NdeI and MluI cut the DNA at the expected sizes of 382 150 for NdeI and 383 149 for MluI. In the Substitution lane, only MluI cut the DNA producing the expected 383 149 bp bands. In the Deletion lane, none of the enzymes cut the DNA, leaving the bands at their original Modified DNA size.

    Journal: PLoS ONE

    Article Title: Efficient method for site-directed mutagenesis in large plasmids without subcloning

    doi: 10.1371/journal.pone.0177788

    Figure Lengend Snippet: Validation of the URMAC method by insertion (I), substitution (S), or deletion (D) of some restriction sites in pUC18 plasmid. (A) Illustration of the Modification Target (NdeI restriction site) relative to the flanking restriction sites and location of the Starter Primers SP1 and SP2. After the first PCR, the Starter DNA migrated as expected, 532 bp on a 1% agarose gel (photo, arrow at right). A 100 bp DNA size ladder is shown in left lane for comparison. (B) Diagram of the strategy for I, S, or D using the Closed Starter DNA circularized from the PCR product in (A) as template and the Opener/Mutagenic Primers . The top photo shows the PCR product, Intermediate DNA , which contained the mutations. The bottom photo shows the Modified DNA after enrichment PCR step using SP1 and SP2. (C) Validation of URMAC mutagenesis for the three different types of mutations by restriction analysis. Fig 2C shows bands of expected DNA fragment size after digestion with respective restriction enzymes. In the control Starter PCR lane, only DNA treated with NdeI enzyme, cut the DNA into two fragments of 382 150 bp. Untreated DNA or DNA treated with MluI remained at the full size of 532 bp. In the Insertion lane, both NdeI and MluI cut the DNA at the expected sizes of 382 150 for NdeI and 383 149 for MluI. In the Substitution lane, only MluI cut the DNA producing the expected 383 149 bp bands. In the Deletion lane, none of the enzymes cut the DNA, leaving the bands at their original Modified DNA size.

    Article Snippet: Successful mutagenesis was confirmed by restriction analysis using NdeI and MluI restriction enzymes (New England Biolabs, Ipswich, MA): 2 μl of Linear Modified DNA for each mutation type was incubated with or without 5 units of restriction enzymes for 30 min at 37°C.

    Techniques: Plasmid Preparation, Modification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Mutagenesis

    Schematic representation of MV-GFP and human λ light immunoglobulin chain displaying MV-λ genomes. ( a ) Human λ chain gene was inserted by exchanging the GFP using a MluI/AatII cleavage site upstream of the N protein. Growth kinetics

    Journal: Molecular Therapy: the Journal of the American Society of Gene Therapy

    Article Title: Converting Tumor-specific Markers Into Reporters of Oncolytic Virus Infection

    doi: 10.1038/mt.2009.92

    Figure Lengend Snippet: Schematic representation of MV-GFP and human λ light immunoglobulin chain displaying MV-λ genomes. ( a ) Human λ chain gene was inserted by exchanging the GFP using a MluI/AatII cleavage site upstream of the N protein. Growth kinetics

    Article Snippet: MluI/AatII (New England BioLabs, Ipswich, MA) digested fragment was subcloned into the full-length infectious clone MV Edmonston vaccine strain p(+)MVeGFP plasmid (kindly provided by Cattaneo, Mayo Clinic, Rochester, MN), replacing the GFP gene.

    Techniques: