Journal: Cancer Cell International
Article Title: Silencing lncRNA ZFAS1 or elevated microRNA-135a represses proliferation, migration, invasion and resistance to apoptosis of osteosarcoma cells
Figure Lengend Snippet: Down-regulated ZFAS1 or up-regulated miR-135a restrains colony formation ability and proliferation of MG63 cells. A The colony formation ability of MG63 cells in each group. B Quantification results in A . C MG63 cell proliferation tested by MTT assay. D EdU assay for detecting proliferation of MG63 cells. E Quantification results in D . F Ki-67 and CyclinD1 protein band and expression in each group of MG63 cells. The data in the figure were all measurement data, in the form of mean ± standard deviation; a vs the sh-NC group, P
Article Snippet: The protein was transferred onto polyvinylidene difluoride membrane and the membrane was sealed with 5% bovine serum albumin for 1 h. The membrane was added with primary antibody Ki-67 (1:1000), APEX1 (1:5000), MMP9 (1:1000) (Abcam, Cambridge, UK), CyclinD1 (1:1000), Bax (1:1000), Bcl-2 (1:1000), MMP2 (1:500) (Santa Cruz Biotechnology, Santa Cruz, California, USA), and GAPDH (1:2000) (Jackson Immuno Research, Grove, Pennsylvania, USA).
Techniques: MTT Assay, EdU Assay, Expressing, Standard Deviation