mirvana mirna isolation kit Thermo Fisher Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher mirvana mirna isolation kit
    ]. (D) The relative expression of <t>miRNA-23a,</t> miR-218 and miR-708 during breast cancer metastasis. Samples were prepared by either mirRICH or <t>mirVana</t> kit and the quantification of each miRNAs were measured by qPCR analysis.
    Mirvana Mirna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28053 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirvana mirna isolation kit/product/Thermo Fisher
    Average 99 stars, based on 28053 article reviews
    Price from $9.99 to $1999.99
    mirvana mirna isolation kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher mirvanap mirna isolation kit
    ]. (D) The relative expression of <t>miRNA-23a,</t> miR-218 and miR-708 during breast cancer metastasis. Samples were prepared by either mirRICH or <t>mirVana</t> kit and the quantification of each miRNAs were measured by qPCR analysis.
    Mirvanap Mirna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirvanap mirna isolation kit/product/Thermo Fisher
    Average 99 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    mirvanap mirna isolation kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher mirvana mirna mimic
    ]. (D) The relative expression of <t>miRNA-23a,</t> miR-218 and miR-708 during breast cancer metastasis. Samples were prepared by either mirRICH or <t>mirVana</t> kit and the quantification of each miRNAs were measured by qPCR analysis.
    Mirvana Mirna Mimic, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirvana mirna mimic/product/Thermo Fisher
    Average 99 stars, based on 1286 article reviews
    Price from $9.99 to $1999.99
    mirvana mirna mimic - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher mirnas
    Drosophila <t>miRNA</t> phenotypes in viability and external morphology. ( a ) Viability defects following ubiquitous expression of SP library under the tubulin-Gal4 driver. Data are displayed as average per cent viability relative to control; at least six independent replicate batches were analysed for each genotype (analysis of variance, post hoc analysis with Tukey–Kramer multiple comparisons test P ≤0.01; error bars, s.d). ( b ) Benchmark comparison of viability phenotypes with Drosophila miRNA null mutants 13 . ‘Confirmed' indicates same viability phenotype shared with SP and null. False negative (‘False –ve') indicates <t>miRNAs</t> where null demonstrated viability impaired phenotypes, but miR-SP lines were viable. False positive (‘False +ve') indicates miRNAs where a phenotype was observed with miR-SP but not in the null animal. ‘miR family' represents miRNAs for which a similar seed sequence is shared and miR-SP lines display a viability phenotype that is only confirmed by an individual family member. The denominator for this chart was 99; we excluded all lines for which there was no null available, lines that were not tested by Chen et al. 13 , mutants removing entire clusters of multiple miRs, or mutants for which complementation was inconclusive ( Supplementary Data 3 ). ( c–h ) Eye morphology defects (arrowheads) following inhibition of miR-92 activity. Genotypes: +/tubulin-Gal4 ( c ), +/Scramble-SP;tubulin-Gal4/Scramble-SP ( d , g ), +/miR-92bSP;tubulin-Gal4/miR-92bSP ( e , h ) +/miR-92bSP;tubulin-Gal4/miR-310SP ( f ). ( i – k ) Expression of miR-2aSP results in wing vein patterning abnormalities ( j, arrowheads) and an overall reduction in wing blade size ( j ; scale bar, 200 μm). Average wing size area was quantified in triplicate samples ( P =0.004; error bars, s.e.m.) from +/Scramble-SP;tubulin-Gal4/Scramble-SP and +/miR-2aSP;tubulin-Gal4/miR-2aSP animals ( k ).
    Mirnas, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22900 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirnas/product/Thermo Fisher
    Average 99 stars, based on 22900 article reviews
    Price from $9.99 to $1999.99
    mirnas - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    ]. (D) The relative expression of miRNA-23a, miR-218 and miR-708 during breast cancer metastasis. Samples were prepared by either mirRICH or mirVana kit and the quantification of each miRNAs were measured by qPCR analysis.

    Journal: RNA Biology

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

    doi: 10.1080/15476286.2018.1451723

    Figure Lengend Snippet: ]. (D) The relative expression of miRNA-23a, miR-218 and miR-708 during breast cancer metastasis. Samples were prepared by either mirRICH or mirVana kit and the quantification of each miRNAs were measured by qPCR analysis.

    Article Snippet: Thus, after the isolation of total RNAs, the following approaches can be used: (1) separation of total RNAs by gel electrophoresis and elution of the miRNA fraction from the gel, (2) removal of large RNA molecules by polyethylene glycol to leave behind miRNA, (3) filtration of RNA molecules based on molecular weight, or (4) the use of a commercial kit (e.g., the mirVana miRNA isolation kit, Invitrogen, MA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    LPS-induced IFNγ in mouse splenic lymphocytes is regulated by the estrogen-regulated miRNA, miR-146a . (A) Total RNA was isolated from freshly isolated splenic lymphocytes using mirVana miRNA isolation kits. Relative expression levels of miR-146a

    Journal:

    Article Title: Suppression of LPS-induced Interferon-? and nitric oxide in splenic lymphocytes by select estrogen-regulated microRNAs: a novel mechanism of immune modulation

    doi: 10.1182/blood-2008-04-152488

    Figure Lengend Snippet: LPS-induced IFNγ in mouse splenic lymphocytes is regulated by the estrogen-regulated miRNA, miR-146a . (A) Total RNA was isolated from freshly isolated splenic lymphocytes using mirVana miRNA isolation kits. Relative expression levels of miR-146a

    Article Snippet: Total RNA, containing miRNAs, was isolated from freshly isolated splenic lymphocytes using mirVana miRNA isolation kits (Ambion, Austin, TX).

    Techniques: Isolation, Expressing

    Polymicrobial infection with periodontal pathogens P. gingivalis, T. denticola , and T. forsythia alters miRNA expression levels in lacrimal glands and pancreas following 12 weeks of infection in rats a) lacrimal glands, and b) pancreas were evaluated

    Journal: Anaerobe

    Article Title: Polymicrobial infection alter inflammatory microRNA in rat salivary glands during periodontal disease

    doi: 10.1016/j.anaerobe.2015.10.005

    Figure Lengend Snippet: Polymicrobial infection with periodontal pathogens P. gingivalis, T. denticola , and T. forsythia alters miRNA expression levels in lacrimal glands and pancreas following 12 weeks of infection in rats a) lacrimal glands, and b) pancreas were evaluated

    Article Snippet: Total RNA including miRNAs were isolated from each tissue and individually processed using a miRNA isolation kit (Ambion mirVana miRNA Isolation Kit, Life Technologies, Carlsbad, CA, USA) following the manufacturer’s protocol.

    Techniques: Infection, Expressing

    Polymicrobial infection with periodontal pathogens P. gingivalis, T. denticola , and T. forsythia alters miRNA expression levels in the gingiva and submandibular salivary glands following 12 weeks of infection in rats. Total RNAs from a) gingiva, b) submandibular

    Journal: Anaerobe

    Article Title: Polymicrobial infection alter inflammatory microRNA in rat salivary glands during periodontal disease

    doi: 10.1016/j.anaerobe.2015.10.005

    Figure Lengend Snippet: Polymicrobial infection with periodontal pathogens P. gingivalis, T. denticola , and T. forsythia alters miRNA expression levels in the gingiva and submandibular salivary glands following 12 weeks of infection in rats. Total RNAs from a) gingiva, b) submandibular

    Article Snippet: Total RNA including miRNAs were isolated from each tissue and individually processed using a miRNA isolation kit (Ambion mirVana miRNA Isolation Kit, Life Technologies, Carlsbad, CA, USA) following the manufacturer’s protocol.

    Techniques: Infection, Expressing

    miRNA expression in NCI-H441 cells and RRL small RNA fractions. A : PAGE analysis of the small RNA fractions purified by the mir VANA miRNA Isolation Kit (Ambion) from NCI-H441 cells ( lane 1 ) and RRL ( lane 2 ). B : the presence of the human microRNAs hsa-mir-183,

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Human SP-A1 (SFTPA1) variant-specific 3? UTRs and poly(A) tail differentially affect the in vitro translation of a reporter gene

    doi: 10.1152/ajplung.00113.2010

    Figure Lengend Snippet: miRNA expression in NCI-H441 cells and RRL small RNA fractions. A : PAGE analysis of the small RNA fractions purified by the mir VANA miRNA Isolation Kit (Ambion) from NCI-H441 cells ( lane 1 ) and RRL ( lane 2 ). B : the presence of the human microRNAs hsa-mir-183,

    Article Snippet: Approximately 1 × 105 cells were recovered by trypsinization, pelleted, and washed with 1× PBS before miRNA preparation with the mir VANA miRNA Isolation Kit (Ambion).

    Techniques: Expressing, Polyacrylamide Gel Electrophoresis, Purification, Isolation

    Drosophila miRNA phenotypes in viability and external morphology. ( a ) Viability defects following ubiquitous expression of SP library under the tubulin-Gal4 driver. Data are displayed as average per cent viability relative to control; at least six independent replicate batches were analysed for each genotype (analysis of variance, post hoc analysis with Tukey–Kramer multiple comparisons test P ≤0.01; error bars, s.d). ( b ) Benchmark comparison of viability phenotypes with Drosophila miRNA null mutants 13 . ‘Confirmed' indicates same viability phenotype shared with SP and null. False negative (‘False –ve') indicates miRNAs where null demonstrated viability impaired phenotypes, but miR-SP lines were viable. False positive (‘False +ve') indicates miRNAs where a phenotype was observed with miR-SP but not in the null animal. ‘miR family' represents miRNAs for which a similar seed sequence is shared and miR-SP lines display a viability phenotype that is only confirmed by an individual family member. The denominator for this chart was 99; we excluded all lines for which there was no null available, lines that were not tested by Chen et al. 13 , mutants removing entire clusters of multiple miRs, or mutants for which complementation was inconclusive ( Supplementary Data 3 ). ( c–h ) Eye morphology defects (arrowheads) following inhibition of miR-92 activity. Genotypes: +/tubulin-Gal4 ( c ), +/Scramble-SP;tubulin-Gal4/Scramble-SP ( d , g ), +/miR-92bSP;tubulin-Gal4/miR-92bSP ( e , h ) +/miR-92bSP;tubulin-Gal4/miR-310SP ( f ). ( i – k ) Expression of miR-2aSP results in wing vein patterning abnormalities ( j, arrowheads) and an overall reduction in wing blade size ( j ; scale bar, 200 μm). Average wing size area was quantified in triplicate samples ( P =0.004; error bars, s.e.m.) from +/Scramble-SP;tubulin-Gal4/Scramble-SP and +/miR-2aSP;tubulin-Gal4/miR-2aSP animals ( k ).

    Journal: Nature Communications

    Article Title: A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs

    doi: 10.1038/ncomms8279

    Figure Lengend Snippet: Drosophila miRNA phenotypes in viability and external morphology. ( a ) Viability defects following ubiquitous expression of SP library under the tubulin-Gal4 driver. Data are displayed as average per cent viability relative to control; at least six independent replicate batches were analysed for each genotype (analysis of variance, post hoc analysis with Tukey–Kramer multiple comparisons test P ≤0.01; error bars, s.d). ( b ) Benchmark comparison of viability phenotypes with Drosophila miRNA null mutants 13 . ‘Confirmed' indicates same viability phenotype shared with SP and null. False negative (‘False –ve') indicates miRNAs where null demonstrated viability impaired phenotypes, but miR-SP lines were viable. False positive (‘False +ve') indicates miRNAs where a phenotype was observed with miR-SP but not in the null animal. ‘miR family' represents miRNAs for which a similar seed sequence is shared and miR-SP lines display a viability phenotype that is only confirmed by an individual family member. The denominator for this chart was 99; we excluded all lines for which there was no null available, lines that were not tested by Chen et al. 13 , mutants removing entire clusters of multiple miRs, or mutants for which complementation was inconclusive ( Supplementary Data 3 ). ( c–h ) Eye morphology defects (arrowheads) following inhibition of miR-92 activity. Genotypes: +/tubulin-Gal4 ( c ), +/Scramble-SP;tubulin-Gal4/Scramble-SP ( d , g ), +/miR-92bSP;tubulin-Gal4/miR-92bSP ( e , h ) +/miR-92bSP;tubulin-Gal4/miR-310SP ( f ). ( i – k ) Expression of miR-2aSP results in wing vein patterning abnormalities ( j, arrowheads) and an overall reduction in wing blade size ( j ; scale bar, 200 μm). Average wing size area was quantified in triplicate samples ( P =0.004; error bars, s.e.m.) from +/Scramble-SP;tubulin-Gal4/Scramble-SP and +/miR-2aSP;tubulin-Gal4/miR-2aSP animals ( k ).

    Article Snippet: Total RNA was isolated according to the miRVana miRNA kit protocol without enrichment for miRNAs (Invitrogen) from ubiquitously expressing miR-SP or Scramble wandering third instar larvae with intestines removed.

    Techniques: Expressing, Sequencing, Inhibition, Activity Assay

    A transgenic library of conditional miRNA competitive inhibitors. ( a ) Second-generation SP elements consist of 20 miRNA binding sites with mismatches at positions 9–12 placed in the 3′-untranslated region of mCherry under the control of 10 tunable Gal4 UAS binding sites. The entire cassette was cloned in an attB vector containing gypsy insulators. phiC31 -mediated positional integration was used to generate a library of 282 inducible lines covering 141 high-confidence Drosophila miRNAs, at defined landing sites on the second (attP40) and third (attP2) autosomes. ( b ) Quantification of endogenous miR-8, miR-9b and miR-13b mature miRNA levels using Taqman quantitative PCR in third instar larvae following ubiquitous expression ( tubulin-Gal4 ) of corresponding miR-SP constructs compared with Scramble controls (Student's t -test: 13b, P =0.02; error bars, s.e.m., n =3 biological replicates with 10 animals per sample). ( c – h ) Targeted expression of miR-8SP ( c ; scale bars, 50 μm), miR-9bSP ( e ) and miR-13bSP ( g ) with ptc-Gal4 in wing imaginal discs ubiquitously expressing tubulinEGFP-miR-8, tubulinEGFP-nerfin1 and tubulinEGFP-K-box sensors respectively. Tissue-specific upregulation of sensor levels was observed in cells along the anterior–posterior boundary of the disc. No change was apparent following expression of a Scramble-SP control ( d , f , h ).

    Journal: Nature Communications

    Article Title: A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs

    doi: 10.1038/ncomms8279

    Figure Lengend Snippet: A transgenic library of conditional miRNA competitive inhibitors. ( a ) Second-generation SP elements consist of 20 miRNA binding sites with mismatches at positions 9–12 placed in the 3′-untranslated region of mCherry under the control of 10 tunable Gal4 UAS binding sites. The entire cassette was cloned in an attB vector containing gypsy insulators. phiC31 -mediated positional integration was used to generate a library of 282 inducible lines covering 141 high-confidence Drosophila miRNAs, at defined landing sites on the second (attP40) and third (attP2) autosomes. ( b ) Quantification of endogenous miR-8, miR-9b and miR-13b mature miRNA levels using Taqman quantitative PCR in third instar larvae following ubiquitous expression ( tubulin-Gal4 ) of corresponding miR-SP constructs compared with Scramble controls (Student's t -test: 13b, P =0.02; error bars, s.e.m., n =3 biological replicates with 10 animals per sample). ( c – h ) Targeted expression of miR-8SP ( c ; scale bars, 50 μm), miR-9bSP ( e ) and miR-13bSP ( g ) with ptc-Gal4 in wing imaginal discs ubiquitously expressing tubulinEGFP-miR-8, tubulinEGFP-nerfin1 and tubulinEGFP-K-box sensors respectively. Tissue-specific upregulation of sensor levels was observed in cells along the anterior–posterior boundary of the disc. No change was apparent following expression of a Scramble-SP control ( d , f , h ).

    Article Snippet: Total RNA was isolated according to the miRVana miRNA kit protocol without enrichment for miRNAs (Invitrogen) from ubiquitously expressing miR-SP or Scramble wandering third instar larvae with intestines removed.

    Techniques: Transgenic Assay, Binding Assay, Clone Assay, Plasmid Preparation, Real-time Polymerase Chain Reaction, Expressing, Construct

    Tissue-specific in vivo screen for miRNAs regulating muscle function and maintenance. ( a ) Screen strategy diagram. Muscle-expressed miRNAs were profiled in the adult thoracic muscle tissue, and silenced by driving corresponding miR-SPs with the dMef2-Gal4 driver. Flight behaviour and IFM morphology was assessed at 10- and 30-day-old animals. ( b ) miRNA microarray profiling of the thoracic muscle tissue. For simplicity, only miRNAs with detectable expression are shown. Red bars denote positive hits in the primary muscle screen. ( c ) Positive hits from the flight screen. ‘Flightless phenotype' was defined at a value above twice the s.d. of Scramble-SP controls in 30-day-old animals (analysis of variance, post hoc analysis with Tukey–Kramer multiple comparisons test P ≤0.001; error bars, s.d., n =3 replicates of 20 animals). Flight behaviour is shown for 10-day-old animals in grey bars and for 30-day-old animals in black bars. *indicates less abundant strand from the mentioned hairpin structure.

    Journal: Nature Communications

    Article Title: A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs

    doi: 10.1038/ncomms8279

    Figure Lengend Snippet: Tissue-specific in vivo screen for miRNAs regulating muscle function and maintenance. ( a ) Screen strategy diagram. Muscle-expressed miRNAs were profiled in the adult thoracic muscle tissue, and silenced by driving corresponding miR-SPs with the dMef2-Gal4 driver. Flight behaviour and IFM morphology was assessed at 10- and 30-day-old animals. ( b ) miRNA microarray profiling of the thoracic muscle tissue. For simplicity, only miRNAs with detectable expression are shown. Red bars denote positive hits in the primary muscle screen. ( c ) Positive hits from the flight screen. ‘Flightless phenotype' was defined at a value above twice the s.d. of Scramble-SP controls in 30-day-old animals (analysis of variance, post hoc analysis with Tukey–Kramer multiple comparisons test P ≤0.001; error bars, s.d., n =3 replicates of 20 animals). Flight behaviour is shown for 10-day-old animals in grey bars and for 30-day-old animals in black bars. *indicates less abundant strand from the mentioned hairpin structure.

    Article Snippet: Total RNA was isolated according to the miRVana miRNA kit protocol without enrichment for miRNAs (Invitrogen) from ubiquitously expressing miR-SP or Scramble wandering third instar larvae with intestines removed.

    Techniques: In Vivo, Microarray, Expressing

    Twelve miRNAs are required to maintain flight muscle structure. Fifty-eight lines were assayed for flight at 10 and 30 days post eclosion, and all lines that displayed significant flight deficits were then assayed for IFM morphology ( a – h ). Sagittal bisections of the adult thorax stained for actin and myosin heavy chain (Mhc) shown at low (top panel) and high magnification (bottom panel). Normal IFM and sarcomere morphology in 10- and 30-day-old Scramble-SP controls ( a , b ; scale bars, 200 μm), late-onset IFM phenotype following miR-1SP expression ( c , d ) or miR-34SP expression ( e , f ), and early-onset IFM defects in miR-987SP animals ( g , h ). A summary of the lines that display flight and IFM phenotypes at 10 days post eclosion ( i ) is shown for comparison with the 30-day results shown in j ; red represents all SP lines that display both flight and IFM defects, whereas orange and yellow represent animals with no detectable IFM morphology defect that were flight impaired or flightless, respectively. ( k–l ) Comparison of miR-34SP and miR-34 Δ / Δ null mutants. Null mutant adults (orange bars) display a stronger flightless phenotype at 10 days but are comparable to miR-34SP (green bars) at 30 days ( k ); error bars, s.e.m., n =3 replicates of 20 animals. IFM sarcomere morphology and Mhc distribution and pattern are comparable in miR-34SP and miR-34 Δ / Δ null mutants at 30 days (displaying 15.7% penetrance ( n =19), compared with 25% in miR-34SP ; l ; scale bar,5 μm). ( m ) NanoString nCounter profiling of adult thoracic muscle. All miRNAs expressed above background values are represented. Only the levels of mature miR-34–5p were substantially reduced in the null mutant. Statistical significance was established in this case by comparing the expression values of miR-34 Δ / Δ to the wild-type control using the NanoStringNorm package in R ( t -test, ** P

    Journal: Nature Communications

    Article Title: A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs

    doi: 10.1038/ncomms8279

    Figure Lengend Snippet: Twelve miRNAs are required to maintain flight muscle structure. Fifty-eight lines were assayed for flight at 10 and 30 days post eclosion, and all lines that displayed significant flight deficits were then assayed for IFM morphology ( a – h ). Sagittal bisections of the adult thorax stained for actin and myosin heavy chain (Mhc) shown at low (top panel) and high magnification (bottom panel). Normal IFM and sarcomere morphology in 10- and 30-day-old Scramble-SP controls ( a , b ; scale bars, 200 μm), late-onset IFM phenotype following miR-1SP expression ( c , d ) or miR-34SP expression ( e , f ), and early-onset IFM defects in miR-987SP animals ( g , h ). A summary of the lines that display flight and IFM phenotypes at 10 days post eclosion ( i ) is shown for comparison with the 30-day results shown in j ; red represents all SP lines that display both flight and IFM defects, whereas orange and yellow represent animals with no detectable IFM morphology defect that were flight impaired or flightless, respectively. ( k–l ) Comparison of miR-34SP and miR-34 Δ / Δ null mutants. Null mutant adults (orange bars) display a stronger flightless phenotype at 10 days but are comparable to miR-34SP (green bars) at 30 days ( k ); error bars, s.e.m., n =3 replicates of 20 animals. IFM sarcomere morphology and Mhc distribution and pattern are comparable in miR-34SP and miR-34 Δ / Δ null mutants at 30 days (displaying 15.7% penetrance ( n =19), compared with 25% in miR-34SP ; l ; scale bar,5 μm). ( m ) NanoString nCounter profiling of adult thoracic muscle. All miRNAs expressed above background values are represented. Only the levels of mature miR-34–5p were substantially reduced in the null mutant. Statistical significance was established in this case by comparing the expression values of miR-34 Δ / Δ to the wild-type control using the NanoStringNorm package in R ( t -test, ** P

    Article Snippet: Total RNA was isolated according to the miRVana miRNA kit protocol without enrichment for miRNAs (Invitrogen) from ubiquitously expressing miR-SP or Scramble wandering third instar larvae with intestines removed.

    Techniques: Staining, Expressing, Mutagenesis