mirneasy mini kit Search Results


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  • 99
    Qiagen mirneasy mini
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 11531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini/product/Qiagen
    Average 99 stars, based on 11531 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini - by Bioz Stars, 2020-04
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    94
    Thermo Fisher mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/Thermo Fisher
    Average 94 stars, based on 108 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-04
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    94
    SABiosciences mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit, supplied by SABiosciences, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/SABiosciences
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-04
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    99
    Qiagen mirneasy ffpe mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Ffpe Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy ffpe mini kit/product/Qiagen
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    mirneasy ffpe mini kit - by Bioz Stars, 2020-04
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    95
    Agilent technologies standard mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Standard Mirneasy Mini Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard mirneasy mini kit/product/Agilent technologies
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    Price from $9.99 to $1999.99
    standard mirneasy mini kit - by Bioz Stars, 2020-04
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    95
    Thermo Fisher mirneasy mini kit mirneasy serum kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit Mirneasy Serum Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit mirneasy serum kit/product/Thermo Fisher
    Average 95 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit mirneasy serum kit - by Bioz Stars, 2020-04
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    91
    Vertis Biotechnologie mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit, supplied by Vertis Biotechnologie, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/Vertis Biotechnologie
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-04
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    95
    Agilent technologies mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/Agilent technologies
    Average 95 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-04
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    91
    tiangen biotech co mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/tiangen biotech co
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-04
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    92
    Transgene Biotek mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit, supplied by Transgene Biotek, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/Transgene Biotek
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-04
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    94
    Millipore mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/Millipore
    Average 94 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-04
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    94
    RAN biotechnologies mirneasy mini kits
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kits, supplied by RAN biotechnologies, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kits/product/RAN biotechnologies
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kits - by Bioz Stars, 2020-04
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    99
    Qiagen mirneasy plasma serum mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Plasma Serum Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy plasma serum mini kit/product/Qiagen
    Average 99 stars, based on 368 article reviews
    Price from $9.99 to $1999.99
    mirneasy plasma serum mini kit - by Bioz Stars, 2020-04
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    Toyobo mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/Toyobo
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-04
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    97
    Qiagen mirnaeasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirnaeasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirnaeasy mini kit/product/Qiagen
    Average 97 stars, based on 585 article reviews
    Price from $9.99 to $1999.99
    mirnaeasy mini kit - by Bioz Stars, 2020-04
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    88
    Thermo Fisher mirneasy mini kit reagent
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Mini Kit Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit reagent/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit reagent - by Bioz Stars, 2020-04
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    99
    Qiagen rneasy lipid tissue mini kit mirneasy mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Rneasy Lipid Tissue Mini Kit Mirneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy lipid tissue mini kit mirneasy mini kit/product/Qiagen
    Average 99 stars, based on 7 article reviews
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    rneasy lipid tissue mini kit mirneasy mini kit - by Bioz Stars, 2020-04
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    Qiagen mirneasy fibrous tissue mini kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Fibrous Tissue Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy fibrous tissue mini kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
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    mirneasy fibrous tissue mini kit - by Bioz Stars, 2020-04
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    99
    Qiagen mirneasy micro kit
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Mirneasy Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy micro kit/product/Qiagen
    Average 99 stars, based on 2576 article reviews
    Price from $9.99 to $1999.99
    mirneasy micro kit - by Bioz Stars, 2020-04
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    99
    Qiagen rneasy
    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or <t>miRNeasy</t> kits. Median RIN values are shown, whiskers indicate interquartile range
    Rneasy, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 15661 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy/product/Qiagen
    Average 99 stars, based on 15661 article reviews
    Price from $9.99 to $1999.99
    rneasy - by Bioz Stars, 2020-04
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    Image Search Results


    RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or miRNeasy kits. Median RIN values are shown, whiskers indicate interquartile range

    Journal: BMC Research Notes

    Article Title: Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue

    doi: 10.1186/s13104-015-1813-5

    Figure Lengend Snippet: RNA integrity values for material isolated from LCM samples, whole tissue slices and cell lines. RNA quality (RIN values) for LCM derived samples, whole tissue slices and cell lines isolated with the RNeasy or miRNeasy kits. Median RIN values are shown, whiskers indicate interquartile range

    Article Snippet: RNA extraction and measurement The following RNA extraction kits were used to isolate total RNA from LCM acquired material: RNeasy® Micro (Qiagen, Germany), miRNeasy Mini (Qiagen, Germany), Arcturus® Picopure® RNA isolation kit (Arcturus, Applied Biosystems, USA.), mirVana™ miRNA isolation kit (Ambion, USA.) and RNAqueous® -Micro (Ambion, USA.).

    Techniques: Isolation, Laser Capture Microdissection, Derivative Assay

    Comparison of RNA extraction kits for microdissected samples. RNA quality for benign and PCa LCM samples are shown for all RNA extraction kits. RIN values are shown as the average of a duplicate experiment. RNeasy and miRNeasy consistently delivered high quality RNA from LCM samples ( a ). Median RIN values were compared between the groups, which were expected to be similar. However, RNeasy generated higher quality RNA than Picopure and RNAqueous (p = 0.01 and 0.08 respectively). Additionally, RNA quality between benign glands and PCa cells within the same patient was variable ( b )

    Journal: BMC Research Notes

    Article Title: Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue

    doi: 10.1186/s13104-015-1813-5

    Figure Lengend Snippet: Comparison of RNA extraction kits for microdissected samples. RNA quality for benign and PCa LCM samples are shown for all RNA extraction kits. RIN values are shown as the average of a duplicate experiment. RNeasy and miRNeasy consistently delivered high quality RNA from LCM samples ( a ). Median RIN values were compared between the groups, which were expected to be similar. However, RNeasy generated higher quality RNA than Picopure and RNAqueous (p = 0.01 and 0.08 respectively). Additionally, RNA quality between benign glands and PCa cells within the same patient was variable ( b )

    Article Snippet: RNA extraction and measurement The following RNA extraction kits were used to isolate total RNA from LCM acquired material: RNeasy® Micro (Qiagen, Germany), miRNeasy Mini (Qiagen, Germany), Arcturus® Picopure® RNA isolation kit (Arcturus, Applied Biosystems, USA.), mirVana™ miRNA isolation kit (Ambion, USA.) and RNAqueous® -Micro (Ambion, USA.).

    Techniques: RNA Extraction, Laser Capture Microdissection, Generated

    Specificity of isolation of exosomes and Ago2 protein complex from follicular fluid and blood plasma. Exosome and Ago2 proteins were isolated from organic-phenol fraction during total RNA isolation using miRNeasy kit and resolved in 8M urea. Protein concentrations were quantified using Bradford assay and total of twenty microgram protein from each group were separated in 12 % SDS-PAGE, transferred nitrocellulose membrane and incubated with specific antibody (CD63 and Ago2). Followed by HRP-conjugated secondary antibody and detected using chemiluminescent substrate. Western-blot results indicate the specificity of exosome and Ago2 protein isolation from follicular fluid (FF) and blood plasma (BP) as indicated in the corresponding figures

    Journal: Journal of Ovarian Research

    Article Title: Controlled ovarian hyperstimulation induced changes in the expression of circulatory miRNA in bovine follicular fluid and blood plasma

    doi: 10.1186/s13048-015-0208-5

    Figure Lengend Snippet: Specificity of isolation of exosomes and Ago2 protein complex from follicular fluid and blood plasma. Exosome and Ago2 proteins were isolated from organic-phenol fraction during total RNA isolation using miRNeasy kit and resolved in 8M urea. Protein concentrations were quantified using Bradford assay and total of twenty microgram protein from each group were separated in 12 % SDS-PAGE, transferred nitrocellulose membrane and incubated with specific antibody (CD63 and Ago2). Followed by HRP-conjugated secondary antibody and detected using chemiluminescent substrate. Western-blot results indicate the specificity of exosome and Ago2 protein isolation from follicular fluid (FF) and blood plasma (BP) as indicated in the corresponding figures

    Article Snippet: Western blot analysis Exosomal and Ago2 immunoprecipitate proteins were isolated from organic phenol part during total RNA isolation using miRNeasy mini kit (Qiagen, Hilden, Germany) and resuspended in 8 M Urea.

    Techniques: Isolation, Bradford Assay, SDS Page, Incubation, Western Blot

    Correlations between mean “fold recovery” (FR) values of miRNAs and base 10 logarithm of miRNA relative abundance in sample (SRA), using yE protocol (A) and yQ protocol (B). yE, miRCURY RNA isolation kit Biofluids modified protocol using yeast RNA carrier; yQ, miRNeasy Mini kit modified protocol using yeast RNA carrier; FR, the fold recovery of each miRNA vs the same protocol without carrier; SRA, the mean relative abundance of each miRNA on samples isolated without carrier.

    Journal: PLoS ONE

    Article Title: Comparison of protocols and RNA carriers for plasma miRNA isolation. Unraveling RNA carrier influence on miRNA isolation

    doi: 10.1371/journal.pone.0187005

    Figure Lengend Snippet: Correlations between mean “fold recovery” (FR) values of miRNAs and base 10 logarithm of miRNA relative abundance in sample (SRA), using yE protocol (A) and yQ protocol (B). yE, miRCURY RNA isolation kit Biofluids modified protocol using yeast RNA carrier; yQ, miRNeasy Mini kit modified protocol using yeast RNA carrier; FR, the fold recovery of each miRNA vs the same protocol without carrier; SRA, the mean relative abundance of each miRNA on samples isolated without carrier.

    Article Snippet: Results In this work we compared different modified isolation protocols (miRCURY RNA isolation kit Biofluids [Exiqon] and, miRNeasy Mini protocol [Qiagen]) named here as E and Q protocols.

    Techniques: Isolation, Modification

    Raw Cq values. Evaluation of Cq values of UniSp2, miR-103a-3p and miR-451a in four samples using different isolation protocols and RNA carriers. y, yeast RNA carrier; m, MS2 RNA carrier; w, without carrier; Q, miRNeasy Mini kit modified protocol; E, miRCURY RNA isolation kit Biofluids modified protocol. Mean and standard deviation values are indicated under each box plot diagram.

    Journal: PLoS ONE

    Article Title: Comparison of protocols and RNA carriers for plasma miRNA isolation. Unraveling RNA carrier influence on miRNA isolation

    doi: 10.1371/journal.pone.0187005

    Figure Lengend Snippet: Raw Cq values. Evaluation of Cq values of UniSp2, miR-103a-3p and miR-451a in four samples using different isolation protocols and RNA carriers. y, yeast RNA carrier; m, MS2 RNA carrier; w, without carrier; Q, miRNeasy Mini kit modified protocol; E, miRCURY RNA isolation kit Biofluids modified protocol. Mean and standard deviation values are indicated under each box plot diagram.

    Article Snippet: Results In this work we compared different modified isolation protocols (miRCURY RNA isolation kit Biofluids [Exiqon] and, miRNeasy Mini protocol [Qiagen]) named here as E and Q protocols.

    Techniques: Isolation, Modification, Standard Deviation

    Correlations between mean “fold recovery” (FR) values of miRNAs and ΔG of each miRNA, using yE protocol (A) and yQ protocol (B). To avoid the influence of GC content in the analysis of ΔG, for these correlations we used only miRNAs with a GC content between 25% and 75% percentile. yE, miRCURY RNA isolation kit Biofluids modified protocol using yeast RNA carrier; yQ, miRNeasy Mini kit modified protocol using yeast RNA carrier; FR, the fold recovery of each miRNA vs the same protocol without carrier; ΔG, the free energy of the most stable secondary structure of each miRNA.

    Journal: PLoS ONE

    Article Title: Comparison of protocols and RNA carriers for plasma miRNA isolation. Unraveling RNA carrier influence on miRNA isolation

    doi: 10.1371/journal.pone.0187005

    Figure Lengend Snippet: Correlations between mean “fold recovery” (FR) values of miRNAs and ΔG of each miRNA, using yE protocol (A) and yQ protocol (B). To avoid the influence of GC content in the analysis of ΔG, for these correlations we used only miRNAs with a GC content between 25% and 75% percentile. yE, miRCURY RNA isolation kit Biofluids modified protocol using yeast RNA carrier; yQ, miRNeasy Mini kit modified protocol using yeast RNA carrier; FR, the fold recovery of each miRNA vs the same protocol without carrier; ΔG, the free energy of the most stable secondary structure of each miRNA.

    Article Snippet: Results In this work we compared different modified isolation protocols (miRCURY RNA isolation kit Biofluids [Exiqon] and, miRNeasy Mini protocol [Qiagen]) named here as E and Q protocols.

    Techniques: Isolation, Modification

    Correlations between mean “fold recovery” (FR) values of miRNAs and GC content of each miRNA, using yE protocol (A) and yQ protocol (B). yE, miRCURY RNA isolation kit Biofluids modified protocol using yeast RNA carrier; yQ, miRNeasy Mini kit modified protocol using yeast RNA carrier; FR, the fold recovery of each miRNA vs the same protocol without carrier.

    Journal: PLoS ONE

    Article Title: Comparison of protocols and RNA carriers for plasma miRNA isolation. Unraveling RNA carrier influence on miRNA isolation

    doi: 10.1371/journal.pone.0187005

    Figure Lengend Snippet: Correlations between mean “fold recovery” (FR) values of miRNAs and GC content of each miRNA, using yE protocol (A) and yQ protocol (B). yE, miRCURY RNA isolation kit Biofluids modified protocol using yeast RNA carrier; yQ, miRNeasy Mini kit modified protocol using yeast RNA carrier; FR, the fold recovery of each miRNA vs the same protocol without carrier.

    Article Snippet: Results In this work we compared different modified isolation protocols (miRCURY RNA isolation kit Biofluids [Exiqon] and, miRNeasy Mini protocol [Qiagen]) named here as E and Q protocols.

    Techniques: Isolation, Modification

    miRNA levels normalized by plasma volume and by an endogenous miRNA. Fold abundance of miRNAs normalized by plasma volume (FR of miRNAs, left column) and normalized by hsa-miR-103a-3p (apFC of miRNAs, right column) and referred to control plasma samples isolated with the E protocol without carrier (in gray). Data were determined in plasma RNA-derived samples isolated with Q and E modified protocols with yeast RNA as carrier (y) or without RNA carrier (w). E, miRCURY RNA isolation kit Biofluids modified protocol; Q, miRNeasy Mini kit modified protocol; FR, fold recovery of miRNAs vs wE protocol; apFC, apparent fold change of miRNAs vs wE protocol. P -values were calculated using Wilcoxon Signed Rank Test, Exact Signification two-tailed, with the IBM SPSS Statistics 20 software. ϕ P

    Journal: PLoS ONE

    Article Title: Comparison of protocols and RNA carriers for plasma miRNA isolation. Unraveling RNA carrier influence on miRNA isolation

    doi: 10.1371/journal.pone.0187005

    Figure Lengend Snippet: miRNA levels normalized by plasma volume and by an endogenous miRNA. Fold abundance of miRNAs normalized by plasma volume (FR of miRNAs, left column) and normalized by hsa-miR-103a-3p (apFC of miRNAs, right column) and referred to control plasma samples isolated with the E protocol without carrier (in gray). Data were determined in plasma RNA-derived samples isolated with Q and E modified protocols with yeast RNA as carrier (y) or without RNA carrier (w). E, miRCURY RNA isolation kit Biofluids modified protocol; Q, miRNeasy Mini kit modified protocol; FR, fold recovery of miRNAs vs wE protocol; apFC, apparent fold change of miRNAs vs wE protocol. P -values were calculated using Wilcoxon Signed Rank Test, Exact Signification two-tailed, with the IBM SPSS Statistics 20 software. ϕ P

    Article Snippet: Results In this work we compared different modified isolation protocols (miRCURY RNA isolation kit Biofluids [Exiqon] and, miRNeasy Mini protocol [Qiagen]) named here as E and Q protocols.

    Techniques: Isolation, Derivative Assay, Modification, Two Tailed Test, Software

    The combination of TLR 3 and 7/8 agonists, and RNA extracted from RV-induced conditioned medium or RV stock caused PGE 2 induction and β 2 adrenoceptor desensitization on ASMCs, but not RNA extracted from HBECs. Control and RV-induced conditioned medium was generated from HBEC (n = 2) and pooled. ASMCs (n = 6) were treated with this pooled control or RV-induced conditioned medium; or untreated (BEGM), poly I:C (50 µg/mL), imiquimod (30 µg/mL) and poly I:C imiquimod (50 µg/mL, 30 µg/mL respectively) in BEGM or in the presence of the control conditioned medium for 3 days (A, B). (Figure C-F) Control and RV-induced conditioned medium was generated from HBEC (n = 3) and pooled. Total RNA was extracted from: control- (53.91 ng/µL), RV-induced conditioned medium (300.25 ng/µL), RV stock (567.35 ng/µL) and cell lysate collected from a sub-confluent 75 cm 2 flask of HBEC (300 ng/µL) using a miRNeasy Mini purification kit and amount of RNA quantified using a spectrophotometer. ASMCs (n = 6) were treated with pooled control conditioned medium (control), or total extracted RNA collected from those sources in the presence of control conditioned medium for 3 days. PGE 2 was measured using an ELISA (A, C) and isoprenaline induced cAMP was measured using a cAMP functional assay (B, D-F). Data represent mean ± SEM. Statistical differences were detected using 1-way ANOVA with Bonferroni post test comparisons to respective BEGM, control conditioned medium only (Control) or Poly I:C *p

    Journal: PLoS ONE

    Article Title: Characterising the Mechanism of Airway Smooth Muscle ?2 Adrenoceptor Desensitization by Rhinovirus Infected Bronchial Epithelial Cells

    doi: 10.1371/journal.pone.0056058

    Figure Lengend Snippet: The combination of TLR 3 and 7/8 agonists, and RNA extracted from RV-induced conditioned medium or RV stock caused PGE 2 induction and β 2 adrenoceptor desensitization on ASMCs, but not RNA extracted from HBECs. Control and RV-induced conditioned medium was generated from HBEC (n = 2) and pooled. ASMCs (n = 6) were treated with this pooled control or RV-induced conditioned medium; or untreated (BEGM), poly I:C (50 µg/mL), imiquimod (30 µg/mL) and poly I:C imiquimod (50 µg/mL, 30 µg/mL respectively) in BEGM or in the presence of the control conditioned medium for 3 days (A, B). (Figure C-F) Control and RV-induced conditioned medium was generated from HBEC (n = 3) and pooled. Total RNA was extracted from: control- (53.91 ng/µL), RV-induced conditioned medium (300.25 ng/µL), RV stock (567.35 ng/µL) and cell lysate collected from a sub-confluent 75 cm 2 flask of HBEC (300 ng/µL) using a miRNeasy Mini purification kit and amount of RNA quantified using a spectrophotometer. ASMCs (n = 6) were treated with pooled control conditioned medium (control), or total extracted RNA collected from those sources in the presence of control conditioned medium for 3 days. PGE 2 was measured using an ELISA (A, C) and isoprenaline induced cAMP was measured using a cAMP functional assay (B, D-F). Data represent mean ± SEM. Statistical differences were detected using 1-way ANOVA with Bonferroni post test comparisons to respective BEGM, control conditioned medium only (Control) or Poly I:C *p

    Article Snippet: RNA Purification Total RNA including micro RNA was purified using the miRNeasy Mini purification kit (Qiagen, Victoria, Australia) as per the manufacturer’s instructions.

    Techniques: Generated, Purification, Spectrophotometry, Enzyme-linked Immunosorbent Assay, Functional Assay

    MEG3 physically interacts with SMO protein. a The overall interaction propensity of MEG3 and SMO protein was predicted by catRAPID. b Predicted interaction between MEG3 (nucleotide positions 0–1200 nt) and SMO protein (amino acid residues 0–720). c RIP experiments were performed using SMO antibody in primary HSCs at Day 0. qRT-PCR was performed to detect pulled-down MEG3. hnRNP-K antibody and IgG were used as positive and negative controls, respectively. d SMO asscociated MEG3 was detected by regular RT-PCR. e Mapping the SMO interaction region of MEG3. Biotinylated RNAs corresponding to different fragments of MEG3 or its antisense sequences (red line) were co-incubated with cell lysates and associated SMO proteins were detected by immunoblotting

    Journal: Cell Death & Disease

    Article Title: LncRNA-MEG3 inhibits activation of hepatic stellate cells through SMO protein and miR-212

    doi: 10.1038/s41419-018-1068-x

    Figure Lengend Snippet: MEG3 physically interacts with SMO protein. a The overall interaction propensity of MEG3 and SMO protein was predicted by catRAPID. b Predicted interaction between MEG3 (nucleotide positions 0–1200 nt) and SMO protein (amino acid residues 0–720). c RIP experiments were performed using SMO antibody in primary HSCs at Day 0. qRT-PCR was performed to detect pulled-down MEG3. hnRNP-K antibody and IgG were used as positive and negative controls, respectively. d SMO asscociated MEG3 was detected by regular RT-PCR. e Mapping the SMO interaction region of MEG3. Biotinylated RNAs corresponding to different fragments of MEG3 or its antisense sequences (red line) were co-incubated with cell lysates and associated SMO proteins were detected by immunoblotting

    Article Snippet: qRT-PCR miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) was used to extract the total RNA from primary cells as well as liver tissues.

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Incubation

    Downregulation of MEG3 in liver fibrosis. a Collagen and α-SMA were analyzed in CCl 4 mice by Masson staining and immunohistochemistry, respectively. Scale bar, 100 μm. b MEG3-1, MEG3-2, and MEG3-3 expressions were detected by qRT-PCR in CCl 4 mice. c Expressions of MEG3-1, MEG3-2, and MEG3-3 were analyzed in primary HSCs at Day 0 and Day 4. Primary HSCs were isolated from the livers of healthy mice. d MEG3 was analyzed in primary HSCs isolated from oil- or CCl 4 -treated mice. e MEG3 was analyzed in primary HSCs and primary hepatocytes from the livers of healthy mice. * P

    Journal: Cell Death & Disease

    Article Title: LncRNA-MEG3 inhibits activation of hepatic stellate cells through SMO protein and miR-212

    doi: 10.1038/s41419-018-1068-x

    Figure Lengend Snippet: Downregulation of MEG3 in liver fibrosis. a Collagen and α-SMA were analyzed in CCl 4 mice by Masson staining and immunohistochemistry, respectively. Scale bar, 100 μm. b MEG3-1, MEG3-2, and MEG3-3 expressions were detected by qRT-PCR in CCl 4 mice. c Expressions of MEG3-1, MEG3-2, and MEG3-3 were analyzed in primary HSCs at Day 0 and Day 4. Primary HSCs were isolated from the livers of healthy mice. d MEG3 was analyzed in primary HSCs isolated from oil- or CCl 4 -treated mice. e MEG3 was analyzed in primary HSCs and primary hepatocytes from the livers of healthy mice. * P

    Article Snippet: qRT-PCR miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) was used to extract the total RNA from primary cells as well as liver tissues.

    Techniques: Mouse Assay, Staining, Immunohistochemistry, Quantitative RT-PCR, Isolation

    Quantification of a model RNA (synthetic Neisseria gonorrhoeae 16S rRNA). Plot shows the percentage of the positive droplets as a function of 16S rRNA concentrations in 100 μL of testing samples. The empty squares are results from clean synthetic sample, and the solid squares are results from samples with 150 ng of human total RNA background.

    Journal: Scientific Reports

    Article Title: Droplet Digital Enzyme-Linked Oligonucleotide Hybridization Assay for Absolute RNA Quantification

    doi: 10.1038/srep13795

    Figure Lengend Snippet: Quantification of a model RNA (synthetic Neisseria gonorrhoeae 16S rRNA). Plot shows the percentage of the positive droplets as a function of 16S rRNA concentrations in 100 μL of testing samples. The empty squares are results from clean synthetic sample, and the solid squares are results from samples with 150 ng of human total RNA background.

    Article Snippet: Clinical Neisseria gonorrhoeae 16S rRNA from Isolated Cell Culture A commercially available RNA extraction kit (miRNeasy Mini Kit, Qiagen) was utilized to extract RNA from bacterial cells, which were cultured from an isolation collected from a patient (Division of Infectious Diseases—Johns Hopkins Medicine).

    Techniques:

    Heatmap of differentially expressed miRs between metastatic and non-metastatic esophageal carcinoma. Sample numbers at the top: 1 and 6–8, non-metastatic group; 2–5 and 9–10, metatatic group. miR, microRNA.

    Journal: Molecular Medicine Reports

    Article Title: miR612 is associated with esophageal squamous cell carcinoma development and metastasis, mediated through TP53

    doi: 10.3892/mmr.2017.6808

    Figure Lengend Snippet: Heatmap of differentially expressed miRs between metastatic and non-metastatic esophageal carcinoma. Sample numbers at the top: 1 and 6–8, non-metastatic group; 2–5 and 9–10, metatatic group. miR, microRNA.

    Article Snippet: The MicroRNA extracting kit (miRNeasy Mini Kit) was from Qiagen.

    Techniques: